LOCUS       DACCQP010000001       108323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCQP010000001 DACCQP010000000
VERSION     DACCQP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 108323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.001"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(219..2111)
                     /locus_tag="IVO87_000001"
     CDS             complement(219..2111)
                     /locus_tag="IVO87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448234.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2108..3835)
                     /locus_tag="IVO87_000002"
     CDS             complement(2108..3835)
                     /locus_tag="IVO87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448235.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3851..4501)
                     /locus_tag="IVO87_000003"
     CDS             complement(3851..4501)
                     /locus_tag="IVO87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7448236.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4705..5001
                     /locus_tag="IVO87_000004"
     CDS             4705..5001
                     /locus_tag="IVO87_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7448237.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSLTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5173..5793
                     /locus_tag="IVO87_000005"
     CDS             5173..5793
                     /locus_tag="IVO87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7448238.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5885..7051)
                     /locus_tag="IVO87_000006"
     CDS             complement(5885..7051)
                     /locus_tag="IVO87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7448239.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7063..7416)
                     /locus_tag="IVO87_000007"
     CDS             complement(7063..7416)
                     /locus_tag="IVO87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7448240.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7544..8566
                     /locus_tag="IVO87_000008"
     CDS             7544..8566
                     /locus_tag="IVO87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7448241.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9005..9439)
                     /locus_tag="IVO87_000009"
     CDS             complement(9005..9439)
                     /locus_tag="IVO87_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448242.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9549..11408)
                     /locus_tag="IVO87_000010"
     CDS             complement(9549..11408)
                     /locus_tag="IVO87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7448243.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11526..12686)
                     /locus_tag="IVO87_000011"
     CDS             complement(11526..12686)
                     /locus_tag="IVO87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7448244.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12993..14417)
                     /gene="araA"
                     /locus_tag="IVO87_000012"
     CDS             complement(12993..14417)
                     /gene="araA"
                     /locus_tag="IVO87_000012"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7448245.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14433..15131)
                     /locus_tag="IVO87_000013"
     CDS             complement(14433..15131)
                     /locus_tag="IVO87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7448246.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15088..16749)
                     /locus_tag="IVO87_000014"
     CDS             complement(15088..16749)
                     /locus_tag="IVO87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7448247.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16967..18367
                     /locus_tag="IVO87_000015"
     CDS             16967..18367
                     /locus_tag="IVO87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7448248.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18619..19698)
                     /locus_tag="IVO87_000016"
     CDS             complement(18619..19698)
                     /locus_tag="IVO87_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7448249.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19784..20743)
                     /locus_tag="IVO87_000017"
     CDS             complement(19784..20743)
                     /locus_tag="IVO87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7448250.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20755..22278)
                     /locus_tag="IVO87_000018"
     CDS             complement(20755..22278)
                     /locus_tag="IVO87_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7448251.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22288..22851)
                     /locus_tag="IVO87_000019"
     CDS             complement(22288..22851)
                     /locus_tag="IVO87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7448252.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22857..23720)
                     /locus_tag="IVO87_000020"
     CDS             complement(22857..23720)
                     /locus_tag="IVO87_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7448253.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23734..24630)
                     /locus_tag="IVO87_000021"
     CDS             complement(23734..24630)
                     /locus_tag="IVO87_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448254.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24805..26076)
                     /locus_tag="IVO87_000022"
     CDS             complement(24805..26076)
                     /locus_tag="IVO87_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7448255.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26066..27583)
                     /locus_tag="IVO87_000023"
     CDS             complement(26066..27583)
                     /locus_tag="IVO87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7448256.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27781..28671
                     /locus_tag="IVO87_000024"
     CDS             27781..28671
                     /locus_tag="IVO87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7448257.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28744..29568)
                     /gene="nadE"
                     /locus_tag="IVO87_000025"
     CDS             complement(28744..29568)
                     /gene="nadE"
                     /locus_tag="IVO87_000025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7448258.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29580..31052)
                     /locus_tag="IVO87_000026"
     CDS             complement(29580..31052)
                     /locus_tag="IVO87_000026"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7448259.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31689..32051)
                     /locus_tag="IVO87_000027"
     CDS             complement(31689..32051)
                     /locus_tag="IVO87_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7448260.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32274..33026
                     /locus_tag="IVO87_000028"
     CDS             32274..33026
                     /locus_tag="IVO87_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7448261.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33166..34089)
                     /locus_tag="IVO87_000029"
     CDS             complement(33166..34089)
                     /locus_tag="IVO87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7448262.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34104..34376)
                     /locus_tag="IVO87_000030"
     CDS             complement(34104..34376)
                     /locus_tag="IVO87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448263.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34846..36471)
                     /gene="groL"
                     /locus_tag="IVO87_000031"
     CDS             complement(34846..36471)
                     /gene="groL"
                     /locus_tag="IVO87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7448264.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36522..36806)
                     /locus_tag="IVO87_000032"
     CDS             complement(36522..36806)
                     /locus_tag="IVO87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7448265.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37031..37693
                     /locus_tag="IVO87_000033"
     CDS             37031..37693
                     /locus_tag="IVO87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7448266.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37769..39061)
                     /locus_tag="IVO87_000034"
     CDS             complement(37769..39061)
                     /locus_tag="IVO87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7448267.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39231..39860
                     /locus_tag="IVO87_000035"
     CDS             39231..39860
                     /locus_tag="IVO87_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP7448268.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39963..40772)
                     /locus_tag="IVO87_000036"
     CDS             complement(39963..40772)
                     /locus_tag="IVO87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7448269.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40827..41696)
                     /locus_tag="IVO87_000037"
     CDS             complement(40827..41696)
                     /locus_tag="IVO87_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448270.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41697..43013)
                     /locus_tag="IVO87_000038"
     CDS             complement(41697..43013)
                     /locus_tag="IVO87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448271.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43010..44845)
                     /gene="walK"
                     /locus_tag="IVO87_000039"
     CDS             complement(43010..44845)
                     /gene="walK"
                     /locus_tag="IVO87_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7448272.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44850..45554)
                     /locus_tag="IVO87_000040"
     CDS             complement(44850..45554)
                     /locus_tag="IVO87_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7448273.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45744..46604)
                     /locus_tag="IVO87_000041"
     CDS             complement(45744..46604)
                     /locus_tag="IVO87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7448274.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46591..47160)
                     /locus_tag="IVO87_000042"
     CDS             complement(46591..47160)
                     /locus_tag="IVO87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7448275.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKIDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47541..47613
                     /locus_tag="IVO87_000043"
     tRNA            47541..47613
                     /locus_tag="IVO87_000043"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47574..47576,aa:Lys,seq:ctt)
     gene            complement(48109..48495)
                     /locus_tag="IVO87_000044"
     CDS             complement(48109..48495)
                     /locus_tag="IVO87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7448276.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48492..49013)
                     /locus_tag="IVO87_000045"
     CDS             complement(48492..49013)
                     /locus_tag="IVO87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7448277.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49015..50049)
                     /gene="sppA"
                     /locus_tag="IVO87_000046"
     CDS             complement(49015..50049)
                     /gene="sppA"
                     /locus_tag="IVO87_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7448278.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50082..51497)
                     /locus_tag="IVO87_000047"
     CDS             complement(50082..51497)
                     /locus_tag="IVO87_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP7448279.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51466..53619)
                     /locus_tag="IVO87_000048"
     CDS             complement(51466..53619)
                     /locus_tag="IVO87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP7448280.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53877..54113
                     /locus_tag="IVO87_000049"
     CDS             53877..54113
                     /locus_tag="IVO87_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7448281.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54132..54347
                     /locus_tag="IVO87_000050"
     CDS             54132..54347
                     /locus_tag="IVO87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP7448282.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55050..55802)
                     /locus_tag="IVO87_000051"
     CDS             complement(55050..55802)
                     /locus_tag="IVO87_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7448283.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(55817..57064)
                     /locus_tag="IVO87_000052"
     CDS             complement(55817..57064)
                     /locus_tag="IVO87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7448284.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(57140..57286)
                     /locus_tag="IVO87_000053"
     CDS             complement(57140..57286)
                     /locus_tag="IVO87_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7448285.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(57390..57701)
                     /locus_tag="IVO87_000054"
     CDS             complement(57390..57701)
                     /locus_tag="IVO87_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7448286.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(57703..57900)
                     /locus_tag="IVO87_000055"
     CDS             complement(57703..57900)
                     /locus_tag="IVO87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7448287.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(58294..60021)
                     /locus_tag="IVO87_000056"
     CDS             complement(58294..60021)
                     /locus_tag="IVO87_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7448288.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(60014..61207)
                     /locus_tag="IVO87_000057"
     CDS             complement(60014..61207)
                     /locus_tag="IVO87_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7448289.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(61394..62038)
                     /locus_tag="IVO87_000058"
     CDS             complement(61394..62038)
                     /locus_tag="IVO87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7448290.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(62132..62266)
                     /locus_tag="IVO87_000059"
     CDS             complement(62132..62266)
                     /locus_tag="IVO87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7448291.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(62268..64400)
                     /gene="feoB"
                     /locus_tag="IVO87_000060"
     CDS             complement(62268..64400)
                     /gene="feoB"
                     /locus_tag="IVO87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7448292.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(64397..64870)
                     /locus_tag="IVO87_000061"
     CDS             complement(64397..64870)
                     /locus_tag="IVO87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7448293.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            65271..65495
                     /gene="nrdH"
                     /locus_tag="IVO87_000062"
     CDS             65271..65495
                     /gene="nrdH"
                     /locus_tag="IVO87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7448294.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            65654..67813
                     /gene="nrdE"
                     /locus_tag="IVO87_000063"
     CDS             65654..67813
                     /gene="nrdE"
                     /locus_tag="IVO87_000063"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7448295.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            67863..68828
                     /gene="nrdF"
                     /locus_tag="IVO87_000064"
     CDS             67863..68828
                     /gene="nrdF"
                     /locus_tag="IVO87_000064"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7448296.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(68917..70056)
                     /locus_tag="IVO87_000065"
     CDS             complement(68917..70056)
                     /locus_tag="IVO87_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7448297.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(70365..71078)
                     /locus_tag="IVO87_000066"
     CDS             complement(70365..71078)
                     /locus_tag="IVO87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7448298.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(71332..72033)
                     /gene="nagB"
                     /locus_tag="IVO87_000067"
     CDS             complement(71332..72033)
                     /gene="nagB"
                     /locus_tag="IVO87_000067"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7448299.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            72267..73799
                     /locus_tag="IVO87_000068"
     CDS             72267..73799
                     /locus_tag="IVO87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7448300.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(73990..75192)
                     /locus_tag="IVO87_000069"
     CDS             complement(73990..75192)
                     /locus_tag="IVO87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7448301.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(75274..75963)
                     /locus_tag="IVO87_000070"
     CDS             complement(75274..75963)
                     /locus_tag="IVO87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7448302.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(76069..76437)
                     /gene="rplL"
                     /locus_tag="IVO87_000071"
     CDS             complement(76069..76437)
                     /gene="rplL"
                     /locus_tag="IVO87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7448303.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(76490..76993)
                     /locus_tag="IVO87_000072"
     CDS             complement(76490..76993)
                     /locus_tag="IVO87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7448304.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(77403..78092)
                     /gene="rplA"
                     /locus_tag="IVO87_000073"
     CDS             complement(77403..78092)
                     /gene="rplA"
                     /locus_tag="IVO87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7448305.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(78196..78618)
                     /gene="rplK"
                     /locus_tag="IVO87_000074"
     CDS             complement(78196..78618)
                     /gene="rplK"
                     /locus_tag="IVO87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7448306.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            78961..79629
                     /gene="sdaAB"
                     /locus_tag="IVO87_000075"
     CDS             78961..79629
                     /gene="sdaAB"
                     /locus_tag="IVO87_000075"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7448307.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            79642..80514
                     /gene="sdaAA"
                     /locus_tag="IVO87_000076"
     CDS             79642..80514
                     /gene="sdaAA"
                     /locus_tag="IVO87_000076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7448308.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(80605..81624)
                     /locus_tag="IVO87_000077"
     CDS             complement(80605..81624)
                     /locus_tag="IVO87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7448309.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(81797..82684)
                     /locus_tag="IVO87_000078"
     CDS             complement(81797..82684)
                     /locus_tag="IVO87_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7448310.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(82761..83306)
                     /gene="nusG"
                     /locus_tag="IVO87_000079"
     CDS             complement(82761..83306)
                     /gene="nusG"
                     /locus_tag="IVO87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7448311.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(83407..83577)
                     /gene="secE"
                     /locus_tag="IVO87_000080"
     CDS             complement(83407..83577)
                     /gene="secE"
                     /locus_tag="IVO87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7448312.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(83601..83753)
                     /gene="rpmG"
                     /locus_tag="IVO87_000081"
     CDS             complement(83601..83753)
                     /gene="rpmG"
                     /locus_tag="IVO87_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7448313.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            83915..84559
                     /locus_tag="IVO87_000082"
     CDS             83915..84559
                     /locus_tag="IVO87_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7448314.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(84615..85118)
                     /locus_tag="IVO87_000083"
     CDS             complement(84615..85118)
                     /locus_tag="IVO87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7448315.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(85131..85177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            85715..86680
                     /locus_tag="IVO87_000084"
     CDS             85715..86680
                     /locus_tag="IVO87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7448316.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(86779..87951)
                     /locus_tag="IVO87_000085"
     CDS             complement(86779..87951)
                     /locus_tag="IVO87_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448317.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(87938..89083)
                     /locus_tag="IVO87_000086"
     CDS             complement(87938..89083)
                     /locus_tag="IVO87_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448318.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(89097..90029)
                     /locus_tag="IVO87_000087"
     CDS             complement(89097..90029)
                     /locus_tag="IVO87_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448319.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            90146..91225
                     /locus_tag="IVO87_000088"
     CDS             90146..91225
                     /locus_tag="IVO87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7448320.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            91222..91851
                     /locus_tag="IVO87_000089"
     CDS             91222..91851
                     /locus_tag="IVO87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7448321.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(91961..92188)
                     /locus_tag="IVO87_000090"
     CDS             complement(91961..92188)
                     /locus_tag="IVO87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7448322.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(92291..94879)
                     /locus_tag="IVO87_000091"
     CDS             complement(92291..94879)
                     /locus_tag="IVO87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7448323.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(94899..95558)
                     /locus_tag="IVO87_000092"
     CDS             complement(94899..95558)
                     /locus_tag="IVO87_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7448324.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(95607..96110)
                     /gene="trmL"
                     /locus_tag="IVO87_000093"
     CDS             complement(95607..96110)
                     /gene="trmL"
                     /locus_tag="IVO87_000093"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7448325.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(96331..97197)
                     /locus_tag="IVO87_000094"
     CDS             complement(96331..97197)
                     /locus_tag="IVO87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7448326.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            97306..97947
                     /locus_tag="IVO87_000095"
     CDS             97306..97947
                     /locus_tag="IVO87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7448327.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(98009..99370)
                     /gene="mgtE"
                     /locus_tag="IVO87_000096"
     CDS             complement(98009..99370)
                     /gene="mgtE"
                     /locus_tag="IVO87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7448328.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(99388..100287)
                     /locus_tag="IVO87_000097"
     CDS             complement(99388..100287)
                     /locus_tag="IVO87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7448329.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(100289..101086)
                     /locus_tag="IVO87_000098"
     CDS             complement(100289..101086)
                     /locus_tag="IVO87_000098"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7448330.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(101064..101768)
                     /locus_tag="IVO87_000099"
     CDS             complement(101064..101768)
                     /locus_tag="IVO87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7448331.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            101899..102474
                     /locus_tag="IVO87_000100"
     CDS             101899..102474
                     /locus_tag="IVO87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7448332.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            102592..103269
                     /locus_tag="IVO87_000101"
     CDS             102592..103269
                     /locus_tag="IVO87_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7448333.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(103348..105156)
                     /gene="pepF"
                     /locus_tag="IVO87_000102"
     CDS             complement(103348..105156)
                     /gene="pepF"
                     /locus_tag="IVO87_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7448334.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(105332..106330)
                     /locus_tag="IVO87_000103"
     CDS             complement(105332..106330)
                     /locus_tag="IVO87_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7448335.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(106556..107209)
                     /locus_tag="IVO87_000104"
     CDS             complement(106556..107209)
                     /locus_tag="IVO87_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7448336.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(107541..107939)
                     /gene="spxA"
                     /locus_tag="IVO87_000105"
     CDS             complement(107541..107939)
                     /gene="spxA"
                     /locus_tag="IVO87_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7448337.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        32327 a        22389 c        18453 g        35154 t
ORIGIN      
        1 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg
       61 agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat
      121 accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga cacgactttt
      181 gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc ttcttcagaa
      241 aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt
      301 cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc gcgtattgta
      361 gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt catcgctttt
      421 tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag gatcaggaca
      481 tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata
      541 ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg aacaaaatcg
      601 tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc gtcgttccca
      661 tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag taccattgaa
      721 aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc gtaacgaata
      781 ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt cttccctgca
      841 ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt aaaatcagtc
      901 attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac tttgtatggt
      961 gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc atccaacact
     1021 tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa acttgcaagt
     1081 tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac atcccctaat
     1141 gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac atagcctaga
     1201 tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc tttccaacca
     1261 gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc agtatgattg
     1321 aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt caataaacct
     1381 aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat catcacgatg
     1441 atcaagctta aagggatcat caataacgcg actaacgtca attgccagct gattgaaagc
     1501 atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt caaattttgc
     1561 tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat atccccattt
     1621 gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa atcatgacgt
     1681 aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa aaagttgaat
     1741 acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat tttatcaaag
     1801 tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc agccattaca
     1861 cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat ttggaagatc
     1921 acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt agacatatag
     1981 ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt tacaccgaga
     2041 tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg acgtgtgttt
     2101 gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct tgataagttt
     2161 cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct tcatctagca
     2221 caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa acggtgatac
     2281 tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg aaatccaacg
     2341 ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc cgtgcaattg
     2401 ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca tggctatcta
     2461 agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat gctttccaaa
     2521 tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc gttccagaga
     2581 acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat tgagtcatgt
     2641 cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat aatcgaggga
     2701 tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg acgatctcgc
     2761 ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca tgatatcgat
     2821 aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact gttttaggat
     2881 tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg tcagctgaag
     2941 cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg atcaagatct
     3001 gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata tctgctatgt
     3061 agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat gtcatcattg
     3121 gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa tctttattcg
     3181 ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc
     3241 ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc atacttttga
     3301 ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact gggatgacaa
     3361 ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc gcgccaatca
     3421 acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt tggatttgag
     3481 tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca aacgcatctt
     3541 ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg
     3601 attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc aaagaaaagc
     3661 cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct acccctttat
     3721 caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg atcaccatga
     3781 aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt agcatctagt
     3841 tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa ttgaattcac
     3901 tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac gaatgcgcaa
     3961 ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca tgatcatttt
     4021 ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt
     4081 ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact tttcttgagg
     4141 cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg ccttccaaaa
     4201 tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca ccatcggatt
     4261 ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca aagtaataaa
     4321 aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg atatccgcta
     4381 atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac tcgattctcg
     4441 cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca
     4501 tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt cattataaca
     4561 cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc gatacattaa
     4621 aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt aaaatagcca
     4681 catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga cttcaataaa
     4741 ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga ttacacaaaa
     4801 aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca attaaaaaat
     4861 ctagatccaa agaaacttta ttatattcta agccttacag gcaggcgatc agaagcatta
     4921 gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg gatggaagct
     4981 cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg tgacaaaaaa
     5041 tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt caaccttatc
     5101 aatatgactt gatagccttt tctttttctc atagttataa caatcatagc aataaaagga
     5161 gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac tgcagaagaa
     5221 tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa tcatccagat
     5281 gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga tgaagaatta
     5341 ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc agaaagtcaa
     5401 caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc tgataaaatc
     5461 gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc ttggttcgat
     5521 accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga aggattagtg
     5581 acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg acaagacttt
     5641 gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt agatcagcta
     5701 ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca aaatgcatta
     5761 gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca tacaaaagag
     5821 acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca tctgtctctt
     5881 tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag attgttgtgt
     5941 accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga atccccaaga
     6001 tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa atcgttactg
     6061 gatggattgc taactgattc cctagtacta atggagagat caagcgtcct tctattgtct
     6121 gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct acaatggcta
     6181 aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg ttcaaaaaac
     6241 ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa ccaatcataa
     6301 acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa gaaacttgac
     6361 tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga
     6421 caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata aacggcatcg
     6481 tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga ctctgaactg
     6541 tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta tcacctgctg
     6601 tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg gttaatttat
     6661 tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt tgttcctgaa
     6721 tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa acaaacagcc
     6781 caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact accggattca
     6841 tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct aaaaattgcc
     6901 agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc aatattaaca
     6961 atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa aaccacgata
     7021 ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat aaaaaatttg
     7081 gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat ttgcaatttg
     7141 tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct ctaaattccc
     7201 attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag caattgttcc
     7261 gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg aatattttcg
     7321 taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct tgtttattgc
     7381 ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt taattgcttt
     7441 caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg tgatagaata
     7501 aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg aaaagataat
     7561 tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc ttgatacaga
     7621 aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta cctatgtagc
     7681 aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag gtggtgccgg
     7741 tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg ctcctttgtg
     7801 ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc ataaaggtga
     7861 agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt caatattcca
     7921 tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact atacggatct
     7981 gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt atacttatga
     8041 tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc caggaacagg
     8101 ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg gtgaattaga
     8161 cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag aattacagaa
     8221 aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta tccgcatttc
     8281 aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg ctgtattcgc
     8341 agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag aaggggattt
     8401 tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca atcagaacac
     8461 cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat tactgaaaaa
     8521 agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa
     8581 tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata ttcctttctt
     8641 tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac tcaaagccga
     8701 tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag ctttccatac
     8761 tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct tttctacaaa
     8821 taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg attttgagta
     8881 agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt gatcactcta
     8941 tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt atcaaagatt
     9001 tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc aattggtctg
     9061 tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc cctgattcaa
     9121 gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa gctttttcaa
     9181 aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact ttcttaagca
     9241 ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca agagtcgatt
     9301 tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca tatttcggat
     9361 gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac tgcacataat
     9421 agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta ttatttattc
     9481 tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt ctatacagct
     9541 cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt ccctgcgaca
     9601 atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg attgaattgc
     9661 gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc cggaagttcc
     9721 tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt ctgttccatt
     9781 agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg cttttctaat
     9841 tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt gtcatttttt
     9901 gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac aactaaagtc
     9961 cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct gctataggaa
    10021 atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt ttttgcagga
    10081 tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt gactaaatga
    10141 atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta agctgactca
    10201 tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg
    10261 aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga agtattgtat
    10321 ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc
    10381 tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc
    10441 atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc atagttttga
    10501 gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc caatggctca
    10561 ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact catcacgaac
    10621 acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag cttctggtat
    10681 ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc cattggttct
    10741 accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt cattttctgg
    10801 ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt atcgggattg
    10861 ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt taatcggatc
    10921 aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa caatcctatc
    10981 acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat atctttcagc
    11041 aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg ccgataactc
    11101 attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt aagggaacct
    11161 aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa gacgagacag
    11221 gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag gtctacggat
    11281 agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa aaagatactg
    11341 atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct gcttatatat
    11401 cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc tcttatttta
    11461 gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc aaatattctc
    11521 tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc agaaattttt
    11581 tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat gtcaatcaat
    11641 atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag ctcgttctca
    11701 ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa tatagtgaat
    11761 cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc tctaaccgta
    11821 ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc tgccaaagct
    11881 tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc taagaactct
    11941 tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca
    12001 gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt
    12061 ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact
    12121 acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac
    12181 tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca acttagcgct
    12241 acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt
    12301 cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt
    12361 tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca
    12421 agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca
    12481 gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa
    12541 tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc
    12601 aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata
    12661 gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat
    12721 ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag
    12781 cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt
    12841 cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg
    12901 ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc
    12961 ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca
    13021 actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga
    13081 tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc
    13141 cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact
    13201 tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct
    13261 cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga
    13321 gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat
    13381 tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta
    13441 tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct
    13501 tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt
    13561 ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag
    13621 aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt
    13681 tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg
    13741 acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca
    13801 acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt
    13861 ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata ggctactgca
    13921 acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg
    13981 attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg
    14041 gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat
    14101 tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt
    14161 gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc
    14221 tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga
    14281 taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact
    14341 tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt
    14401 tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt
    14461 tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat
    14521 gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc
    14581 attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc
    14641 cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct
    14701 gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc
    14761 gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt
    14821 gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa
    14881 cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata
    14941 tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg
    15001 ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta
    15061 tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt
    15121 tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat
    15181 gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga
    15241 aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa
    15301 agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc
    15361 ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg
    15421 tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt
    15481 ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat
    15541 ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc
    15601 ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca
    15661 atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat
    15721 ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga
    15781 agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc
    15841 gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt
    15901 accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca taccggttcc
    15961 tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat
    16021 cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgatacta gaactttagg
    16081 caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt
    16141 tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc
    16201 tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt
    16261 caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc
    16321 gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc
    16381 ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa
    16441 tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc
    16501 gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca
    16561 aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc actcataact
    16621 accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc
    16681 aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac
    16741 ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag
    16801 tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt tatttacaag
    16861 aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa
    16921 aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa
    16981 aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata
    17041 tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg
    17101 taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg
    17161 ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc
    17221 acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt
    17281 tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc
    17341 tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca
    17401 agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagtgtt
    17461 gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt
    17521 atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt
    17581 ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt
    17641 cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt
    17701 gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca
    17761 atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg
    17821 gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg
    17881 ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg
    17941 cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg tactggatgc
    18001 tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg
    18061 cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag ctgtccgcgg
    18121 gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct
    18181 ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat
    18241 ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt
    18301 agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca
    18361 ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt
    18421 ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact
    18481 tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc
    18541 ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag
    18601 gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac
    18661 aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg
    18721 tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc
    18781 taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata
    18841 gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat
    18901 gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg
    18961 tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca
    19021 cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa
    19081 caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac
    19141 atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat
    19201 cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat actgattact
    19261 tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc
    19321 tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg
    19381 taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt
    19441 gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc
    19501 tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat
    19561 tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg
    19621 aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat
    19681 atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg
    19741 agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa
    19801 caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt
    19861 gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga
    19921 tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt
    19981 gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat gattctttta
    20041 ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg
    20101 cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat
    20161 ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc
    20221 cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt
    20281 caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt
    20341 ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca
    20401 ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta
    20461 tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt
    20521 caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc
    20581 ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc
    20641 ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg
    20701 cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat
    20761 tttctttatt ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg
    20821 tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt
    20881 tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa
    20941 atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag
    21001 ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca
    21061 atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa
    21121 gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat
    21181 taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg
    21241 taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca
    21301 atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt
    21361 ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc
    21421 acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt
    21481 cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc
    21541 aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg
    21601 atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat
    21661 tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac catattcata
    21721 agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac accataattt
    21781 aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt
    21841 tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt
    21901 caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc
    21961 aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt
    22021 cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat
    22081 gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc
    22141 agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac
    22201 aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac
    22261 tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga
    22321 tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag
    22381 cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac
    22441 tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt cagaaatcac
    22501 atatatccag gataaaagaa acatggaata aataagtaaa actacatatc cactaattga
    22561 aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa tgatcgttaa
    22621 cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga aaaaataaat
    22681 gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag cacatgtagc
    22741 aggcacgata gtaataacag gcaaacacac gattacccag agaataccta ataagatgta
    22801 atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca tcttcctcac
    22861 tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa aatataaacc
    22921 actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata aaaagaaatg
    22981 ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga actacttgtc
    23041 aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat ccctaatgat
    23101 aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa ctcacttaat
    23161 ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc
    23221 atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata agtatcaagc
    23281 atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa tgggatcatc
    23341 attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat acgagcaaat
    23401 gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc agcaaaaatc
    23461 actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat atagttatct
    23521 agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt ttcgtgttta
    23581 aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat aactagcata
    23641 ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac agtaactgta
    23701 atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt ctttccgtga
    23761 catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa ccgaagacag
    23821 tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa tatattgaac
    23881 agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt ggtcaaacac
    23941 ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa cgatctgagg
    24001 tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag cagattcata
    24061 caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa caccaaaatt
    24121 cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg
    24181 agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag
    24241 caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa tgtaaaatgt
    24301 cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg ctaataaacc
    24361 aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt
    24421 aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa aatttcttga
    24481 tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac
    24541 agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag cacgatcagg
    24601 acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca ttttgataaa
    24661 atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc tgtgattttt
    24721 gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat cttgccaaca
    24781 gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg catcatctgc
    24841 atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca gtggattgat
    24901 gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct ctgcatagtc
    24961 catttgtttc gataattcag atacaatagg atcatttttt acttcttcat cttttgctac
    25021 tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa tttcagttgt
    25081 attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg tacttgccgg
    25141 aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg
    25201 taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta accatggccc
    25261 attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg cgccagaaat
    25321 cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta acgttttaag
    25381 gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt taccgccatt
    25441 tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa aacctgatac
    25501 atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc aagtagtagg
    25561 tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat acatatacat
    25621 gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta attcaatagc
    25681 cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt ctaatggttg
    25741 taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa taaagtcagg
    25801 tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc aagtcatgat
    25861 atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa cgatttcttg
    25921 caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca cttctttatc
    25981 agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg ttgcagctac
    26041 caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct tcatctgata
    26101 aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt ttcccatctt
    26161 cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga agattagttg
    26221 cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat gataattcag
    26281 acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat atgacaagtt
    26341 ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa tcttccgtcg
    26401 catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac acagcagctt
    26461 gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct gtcctgattg
    26521 gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct gcatgtttca
    26581 ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag ttatcttcta
    26641 gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct gaatgatacc
    26701 aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc ttcgccttga
    26761 ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa
    26821 gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac aaataatcta
    26881 tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta ggcatttgcc
    26941 gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc atcgccttag
    27001 ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc tgccaaggac
    27061 cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct tgaccattcg
    27121 aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg actaaattac
    27181 gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg gcccccacat
    27241 gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt gtccgccaag
    27301 ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc caacgaaatc
    27361 ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct tttaccaagt
    27421 gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc tgataaatcc
    27481 cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc ggatcaactt
    27541 tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac ctcctttaat
    27601 ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg ttaaacttac
    27661 ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca aataatttaa
    27721 tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga ggtgatatat
    27781 atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc tagtaaaaca
    27841 aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga tttggcaaaa
    27901 gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga ggaagcaaac
    27961 atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt cgataaaatc
    28021 gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac atcgatacct
    28081 attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac gatccatgat
    28141 tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat ggatgcccgc
    28201 attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt aaatacagag
    28261 gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc tttttcaaat
    28321 gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg aacatggacg
    28381 agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta tccagataat
    28441 cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac gaatatgaat
    28501 ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga cacttggcat
    28561 ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt atttggtaaa
    28621 ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg atatttttct
    28681 tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt ttttgcttta
    28741 ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt
    28801 gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc cttctagata
    28861 atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca ttggtttccc
    28921 atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat tcaatgcttg
    28981 aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt cggctccacc
    29041 atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg tccctaatac
    29101 ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac gtgctttgat
    29161 atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt gtacttgtgc
    29221 atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa agtcaagagc
    29281 agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag cgataaattg
    29341 atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg ccaaacggcc
    29401 tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa gaaacggata
    29461 ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt ttggatcaat
    29521 tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt attctcctcc
    29581 tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat agccatttta
    29641 tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa gtctcgttta
    29701 tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc gtttagtgca
    29761 ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg gcgagctgta
    29821 aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa gatagcttct
    29881 tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt cccatcaaaa
    29941 tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc tgcattgctt
    30001 gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac tagcttgaat
    30061 actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac accccataca
    30121 tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa
    30181 gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac acgcttagat
    30241 atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt gtcacctaat
    30301 tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata agtgtcgact
    30361 aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata atcatttcca
    30421 taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa tatcttccca
    30481 gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt tccccagact
    30541 gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc ttctccgatg
    30601 acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac catattcaac
    30661 agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat caatggttca
    30721 tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt
    30781 tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta agcaatatcg
    30841 gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc
    30901 gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc atgtaaatca
    30961 gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc cgtatgcagc
    31021 gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg gcaagttttc
    31081 atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca ttttttctga
    31141 aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc taaaactatc
    31201 ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca atgtatccga
    31261 gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta gattttccta
    31321 ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat gtctctacaa
    31381 cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt gcttcttatc
    31441 cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc gaattatgac
    31501 aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt taaaaagata
    31561 caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc
    31621 tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt
    31681 agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt
    31741 ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg
    31801 acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg
    31861 atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc
    31921 agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag
    31981 aattccacta ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg
    32041 attagcttca tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg
    32101 ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat
    32161 ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac aattttttct
    32221 tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt
    32281 acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg
    32341 atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca
    32401 aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg
    32461 atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc
    32521 atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac
    32581 agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt
    32641 ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat
    32701 acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt
    32761 atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag
    32821 aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttatctt
    32881 acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg
    32941 atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa
    33001 atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa
    33061 aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt
    33121 ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa
    33181 cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc
    33241 tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa
    33301 attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc
    33361 tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc
    33421 tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga
    33481 tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact
    33541 tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc
    33601 tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc
    33661 acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg
    33721 agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc
    33781 tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc
    33841 ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac
    33901 tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc
    33961 gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc
    34021 attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat
    34081 gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca
    34141 gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc
    34201 ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact
    34261 atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca
    34321 aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca
    34381 agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg
    34441 ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt attttaaatt
    34501 gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga tatttacttt
    34561 taatagataa aatattataa ctattaaaaa taactatttt cattataatt acacacaaaa
    34621 aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacag
    34681 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
    34741 ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc tctttttatc
    34801 tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca tcataccgcc
    34861 catcattgat ggatccattg caggagctgc tggcgctgct ggttctggtt tatctgcaac
    34921 gactgcttct gtcgttaata acaaggcaga aacggaagcg gcattttgca aggcagaacg
    34981 tgttacttta gttgggtcta cgatacctgc ttcaaccatg tttacccatt ctccagtagc
    35041 agcattgaaa ccagtgccta gttccacgtt cttcaattta tcaacaatca ctgatccttc
    35101 ataaccagcg ttttcagcga tttgacgaat tggttcttct aaagcacgaa caacgatctt
    35161 gatacctgtt gccacgtctc cttctgcttc tacagcagaa actttgctga taacgttcac
    35221 tagagctgta ccaccacctg agaccattcc ttcttctact gcggcacgtg tagcattcaa
    35281 tgcatcttca atgcgcaatt ttaattcttt caattctgtt tcagtagctg caccaacttt
    35341 aacaacagct acaccacctg ctaatttagc taagcgttct tgtaattttt cgcggtcaaa
    35401 atcagaagtt gtttcagcaa tttggttttt gatcaattgc acacgagctt cgattgcttc
    35461 tttttctcca gaaccttcta cgatcgttgt attgtcttta tctacaacaa ctttagaagc
    35521 atttcctaag ttttcgattg ttgcatcttt caattctaaa cctagatcgt cagtaattac
    35581 tgtaccacct gtcaaaatag cgatatcttc aagcattgct ttacgacgat cgccgaaacc
    35641 aggagctttt acagctacta cgttgaatgt tccacggatt ttgttcaata caagagttgg
    35701 caatgcttca ccatctacat cgtcagcaat aatcaacaat ggacgtgatt gttgcaagat
    35761 ttgttctaac aatggcagaa tatcttggat gttagagatt tttttgtctg tgatcaagat
    35821 atatgggttt tctagaacag cttccatttt gtcgttatct gttaccatat attgtgataa
    35881 gtaaccacga tcaaactgca taccttctac aacatctagt tctgtttcga tcccttttga
    35941 ttcttcgatt gtgatgacac cgtcgtttcc aactttttcc attgcatcag caatcaaatg
    36001 tccaacttta tcagaacccg aagaaacagc tgctacttgt gcaatggctt cttttgaatc
    36061 aacaacagtt gagatattat gcaattcttc gactgctgct tttgttgcca attcgatccc
    36121 gcgacgaatg cctagaggat tagcacctgc agttacgttt tttaaacctt cgcgaacgat
    36181 tgcttgtgtc agaacagttg ctgtcgttgt accgtctcct gcaatatcat ttgtttttga
    36241 tgctacttct gatacaagtt ttgcacccat gttttcaaaa tgatcttcta attcgatttc
    36301 ttttgcgatc gtcacaccat catttgtgat caatggagag ccgtaagatt tttctagtac
    36361 gacatttcgg cctttaggtc ctaatgttac tttcactgta tctgctaatt tatctacccc
    36421 gcgtaacata gctgcgcgtg cgtcttctgc aaatttcaat tcttttgcca tgattgattc
    36481 acctcattga ttatttttga ttattttttt atcttccaaa tttactcaac aatcgccatg
    36541 atatcttttc ctgcgacgat cagatattct tttccttcgt attttacttc tgtaccggcg
    36601 tatttttcga acataacttg gtcgcctgct tttacagcgg caggaacttt ttcaccgttt
    36661 tctaacaaac ggccttcacc gactgcaaca actgtcccag tttggggttt ctctttagca
    36721 gcagaagcta aaacgattcc tccgacagtt ttttcttctt cttttgcgac ttcgataatg
    36781 acgcgatcac ctaatggttt taacacgata aatccctccg ataactcttt ttttaatctg
    36841 ttagcactct taaccttcga gtgctaacct acatttttaa taatactcat tctccctctt
    36901 ttttgcaagc ttttaaatca aagaaaagaa attttattct gacagctgct tattgaaatt
    36961 atgatataat tgaaatcaat cgatataagc ttgccttgca tgcttgttat cccttaaaag
    37021 gaggaattac atgtctttaa aaaaatatag cttcctcagc attttttgct atggtctaat
    37081 ttttatctca cccttgatcc tagcttcgat aggacttgta aaatctacgt ctgaattgat
    37141 cacagcaact gcagtcatgt atatactagg cgccgttgtc ctagctattt tttattttaa
    37201 gcaacgtgaa cccttagcga tcgaatcagc ggtaaaaaaa gcttctgctc caaagattgt
    37261 tatttatgga ttggttggaa tctttgtcgc attgattctg caaagcatcg ctgtcatgtt
    37321 agaatctgta ttgtttggca aagcaacccc ttccgagaat acacaaaata tcatccagat
    37381 gatcatggaa gcacccgctt ttattttggc gacaactgtt gctggaccca tcatggaaga
    37441 gttcgtattc cgccgcagta tactggggat catcagtcga tattcaaatt tttgggtcgg
    37501 agcagtgatc agctctttgc tgtttgcgtt tgctcataat gatggtcatc tgctaatcta
    37561 ctttttccta ggtttcttct tcagcctaga gtacaaagca actggtcgga tctggacatc
    37621 gatgatcaca cacgtaggaa tgaacacatt agttgttctt gttcaattag ctgtccaaaa
    37681 agggctagta taaacatgac tataaatact cctgtcatcc tagattggtg attttcccaa
    37741 tctaaagcga caggagtatt tttgtttttt agtcgttcat cttttctgct gctccatcat
    37801 tttttgtctg tctttccttt ttgcagtttg cagaatctat ttttttcaaa aatgggaaat
    37861 aaatgaaaaa actaattgac aacacgatac cttgtaaaat cgctgcaggc actccgccta
    37921 ctaagaaacc cgagaggatc ggcggtgttg tccaagggac ttggactgct gtaaataaag
    37981 gaacgaatcc aaaatataaa gcggaatagg taatcaatcc tgaaacgacc ggtgcgaaaa
    38041 taaaaggaac agccatcatc gggttcatca cgatcggtgt ggaaaaaata attgattcat
    38101 tgatgttgaa aaaggctggt aaaagagcaa gatttcctaa ttctctgtat ttcttcgatt
    38161 tcgcaaaaac cgtcataaaa aatacagccc ctaacgtcaa tccagcaccc gtaaccgtca
    38221 tgaactgatc gagaaattgc tgagtaacga tatgtccgcc atttgccacc gtcaactctt
    38281 gtcctgaggc taagatttct gcatttgcta atgaattcgc ttgcaagacg ggactaacca
    38341 acccattgac catcgcagat ccatgaagac caaataacca taaaaacgga acaaaaaaac
    38401 ctaataaaag cacaccaaac aacgtgtcta tgaacgattg aatgggaagc tggacatatg
    38461 tccatactcc ttgaacaaca gtcattctga acaaatgttg aaaaaccgct gaaccgataa
    38521 gcgtaaaagt gactataaca atgattggaa tcaatgaagt aaaagactgc caaacatttt
    38581 caggtatctt ctctctttgc ttccaccctt tcgcaagtct tactaaacgt gcatagcccc
    38641 atccgatgat gagcccagtt gtaactgctc ctagtatccc ttcagcacct gtccagacag
    38701 tatctatctg agtatttagt ggctgaataa gcagtagata agcagacaag gctgttatgc
    38761 cgactggtaa gcgatcgatg tcttcttctt ttgcataagc gtatgcaatg gcaaaaacga
    38821 tccaaaaggc tatcacatca aatgtagcct ggtatacttg tgtaaaatac atagaaaatc
    38881 ctgttttgtc tatccactga gcgatgtaat ccacaggaaa atgagcaaat aagaaaaaca
    38941 gcgagccaac aataattatt gggatcgtgt aagtcattcc attacgcaaa gcagttaagg
    39001 gtctaagatt cacaacagat gttatcattg gtaaaaaggc acgatctaac cactctttca
    39061 tttcttatct ccttcaactt ctatttttat caatcagttt tcttaattta actataccca
    39121 atgatcacga accatgaaag gaatgcgcac tgagcttttc aaaaatcgga ttctttgacg
    39181 aatattcagc aactgcttac aatggacagg aataaaaaag gaggtttttt atgaaaattg
    39241 cactcattgg agctactggc catgcaggtt catttatttt agacgaagca ttgtccagag
    39301 atttagacgt gacggcaatc gtgcgtcatc cagaaaaatt gccatcagat gttccgttta
    39361 tacaaaagga tttatttgag ttgacaacta ctgatcttgc atcttttgat gttgtcgttg
    39421 atgctttcaa cgctcccaaa ggaaaagaag aaatgcatca aacttctttg tcccatttga
    39481 ttgagatgct tgaaggaacc gatacacgct taattgtcgt tgggggtgcc tcttcgttat
    39541 tcttagatac agaaaagacg actcgtatga ttgaccaagt acctaaaaat gcacccttct
    39601 atccaacagc tttcaatatg tacgaaggat tgttaaaatt gaaacaatct aaaaatctac
    39661 attggactta tatcagtcca gcttctttat ttgatccaca tggcaaacgt acaggtactt
    39721 atttgttaag tgatgattat ttacaaagaa acgctgctag cgacagtacg atcagcatgg
    39781 cagattacgc gattgcatta gtagatgaga tactggatga gaaacatgaa aagcagcaca
    39841 tcagtgtagt cagtcgataa tacggttatt tttttaatat gctaaaaaaa taaaaataga
    39901 agataaaacc aaccctgatt gattttatct tctgcttttt atatttttat tgattgaact
    39961 gtttaaatcg aaaatagctc agttgcttca tctggatctg tatcatagat ttcaaaatca
    40021 tgtccaacgc caaaatcttt ttctttcaaa acattttcca ttgttaaatg agcaagttct
    40081 ttcatattgt tttccttgct aagatggcca agatagattc gtttggtccg attaccgatc
    40141 acatcagtca tgacaagtgc accgtcttcg ttggaaagat gtcctctatc tcctaaaata
    40201 cgctgtttta aattccacgg ataaggtccc atgcgtaaca tctcaagatc atgattactt
    40261 tccatcaaat aaccatcggc attttcaatg acacctcgca tataatcact gcaatatccg
    40321 gtgtccgtca acatgacgaa tgaccgattt tctttatgga aacgataaaa ttgcggagcc
    40381 gctgcgtcat gggagacgcc gaaactctca atatcgatat cgccgaatgt tctgactttc
    40441 cccatctcaa atagatgttt ttgttccact ggtacatttc caatcagtgg ttccatcgct
    40501 tcccaagtct gctgattggc aaaaacatcc aaatgatact tacgagccaa gacacccacg
    40561 ccatgtatat gatctcgatg ttcatgcgtc acaagtattc catctaattg gtccggtgtc
    40621 cgatcaattt gagccatcaa ggaagtgatt tttttaccgc taagtcctgc atcgaccaaa
    40681 agccgttttt tttcactttc gatataaagt gaattacccg aacttccact tgctaggata
    40741 ctgattttaa aagctggttt ttcttcagcc atttgactcc tacttccctt cttactttgt
    40801 aaactttttt cattatactc gcttttttat tgatcttcca cttttggtac agtattgttt
    40861 gtcacgatcg tattgctgaa agcgttgacg ctctcaattt ggaccacttt atcgcttgtt
    40921 tcgatcgcta catgccaaac aggaacatac acattttttt cccggacttt cagaatacag
    40981 gaataagcta gctgacgcca taaaattttt gatcctcttg gtattttgtt attgatgtac
    41041 aatgtattga ttgcttcctc ttccgtatac agttccgtct tttcgcgcag ctgctcgata
    41101 tcagataaat gcgtctgcat gtatttcgtg atgctataat cattatcgtt tttttctaaa
    41161 gaaagaacga tttttgctgt atcatcattg aatgggatgc cttcatactc ttggcttgct
    41221 tggatttcta gtggttcttc ttctaaatga gaaaagtttt tcgtgtacac ataatcatta
    41281 ccgaataaaa tattgtcttt gttagataaa aaatcttcca aggaactact aattttatct
    41341 ggatcgatca cgttcttttc agcatctgaa ctggataaag atttcgtcaa aacatcaccg
    41401 tctaaaaaca caccacttgc cagcaagccg cttttaccag ccttgcgata attcgctaat
    41461 gaagtgctaa gattggtctg ctccgcgcta agataatagc cttgcttgtt ctcagacgaa
    41521 atcttccccg tgtaagtgat atcgtcgcta gccagacgtt gttggattga ttcagtctga
    41581 ttagagcgaa tgacattgtt ttcttggtat accgcgtcat gataactgct gaataaaaac
    41641 acattcaacc ctaaaaaagc gacgaaaaat atccattcga tttttttgaa atccaattaa
    41701 ttcgcctcca atccaggtaa tttttccaga aggtcgttag ctgaatacca gttatcttta
    41761 tatttcacat accattcagg taagagatca acgacacgat tcgtttcttt gacattttgc
    41821 catgtatagc caacgatcac tgaaccaatc ttgttgctat ctgctccctt agcttttaac
    41881 tgctggatga tttcattcgt acttgcaagt tctacttctt cgtctgaagg tatcggcact
    41941 tgaatcgtat tcaaactggt ttggatattc acttgcacag aaccattcgt ttcaccgcca
    42001 acttccacac caatctgtcc tctgctgtct gtcccaaaaa ccgggaagcc ttccacgaat
    42061 atccgataat cgatcgtatt gtcactgcgg tcaaaatatc tgaggttccc taaagaactg
    42121 cctaatttgc taatataggg gaaactttca gaaaatacac tagcattttc ttttttctct
    42181 ggcagctctc cacgaaaatc aactacttgc gtttccgaat ggatagacat tgtttcattc
    42241 ccaccgtaaa aaatcaattc attgctttca tcattatttt tcacgctatc tggtttctgg
    42301 aaaaaagcat ttctaaaaag tgtatagggt tgctgtgaca ggatataact atacttcttc
    42361 agtttgatcg aatgtttcgt agcatattgc gtgtcgatcg tcgatggttc tgttgtcatt
    42421 tctgtccatt ttgtgtcttt tgctgttaat tctttattga tatctgtcca attcaacgaa
    42481 atatttgctt catatacgag atggttcgaa taattcaaaa atcggatttt tttatttcct
    42541 tgatcgaatt gtattctagt aaaatacata ttttcgcttt cttttgccaa gttcgacaca
    42601 tctaaatcta aatcaaatgc ttccttgtat tctcttagca aaaaaggcgc attataggcc
    42661 aactcaaaac cagagttcag atttttcacc tgttcaaact gtgatttgtc tcctgaaact
    42721 acttcagaaa gctttccaaa tcttgcttta tcgatgatca attgcaatcg gtacacgaga
    42781 ttttcactgt tggtttcttg gatcattccc gaatggtgcc atgtaacatg aagcggaaga
    42841 aagacttcgg aagtttttcg attgctttga gactcgatca tttgagtgtt tgtctcatct
    42901 acgttgattg aactttttcc tgctgggctc agccagattg cataagagaa gtatatgctt
    42961 aacgcaacca tcaaaatgag tcctatacga acgacttttt cagagattct cattcccacc
    43021 attcctcctc ataaggttca tatggcagcg agatataaaa agtcgaccct tttccttcct
    43081 tgctctctgc ccaaatagcg cctcgatgcg ccttaatcac ttctctagaa atagctaatc
    43141 caagtcctgt cccaccttgt gctcttgcac gtgctttatc tactcgatag aaccgctcga
    43201 acactcggtt cagatctttt ttaggaatcc ccaatccttg atcggtgata cttaaaatca
    43261 cgttattatg cgtttctaat aagcgacaag taatcgttcc cccatctggt gaatacttga
    43321 tcgcgttatt catgatatta tcgactactt ggatgatttt atctgtatct acttcgaccc
    43381 aaagatctct ttgcgtaaat tcacgacgga tcttataatt tttttcctga tttcccacca
    43441 tcatatcaaa ccggtcaaga acaaaattca ctaactcgtt gaaattgaca tattctaatt
    43501 gaagctgggt attacctgta tccatgcgag atagatttaa gaggtcattg atcattcgaa
    43561 tcattcggtc tgtttcatct agcgttacct tcaaaaaatt tggtgctact tcctcgtctt
    43621 tccacgcacc ttcgctcaat gcttcgatat aactacgcat acttgtcaac ggtgtacgaa
    43681 gttcatggga aacattagag acgaattcac gacgttcccg ttctgttttt tcttgttctg
    43741 tcacatcatg caagacagct actaatccgc tgataaaacc tgattcgcga cggatcatag
    43801 aaaagtctac tcgcaagatc atctgatcac tttccaatgt actggttgaa cggtcaagta
    43861 ccatttcgtt tggatcttct aataatttcc gcaacgtata ttcttcttcg atatctaaaa
    43921 gtgtcaaaat cgattgtccg attgcttcct cgtttttgat atctagcaag gaaacagcca
    43981 tatcattgat cgtaatgact ttacccctgc gatctgtcgc aatgacgccg tctgtcatat
    44041 gggatagcac gctatccaaa cgattgcgtt cagcttccat tgtatcttga gcttcttcaa
    44101 tacgttcagc taactgattg aaggtatcag ccagctgtcc aagttcatct tgtccatgca
    44161 ctttgacttt ccgactgtag tctcctctag cgattcgaat ggcttgttct ctcatttcac
    44221 cgatcggctg tgtaatcgaa cgagcaacta aaacagatac gatcattgaa ataaatgccg
    44281 caattagtga ggcagtaaag aaaatgactg ctgtgttatt gatttccgaa tatttttgtt
    44341 cgatattact tttgacgtaa agagccccga taacggcatc gccggtcggg gacaggatcg
    44401 gttgaacatt gatattcacc cgtttatcgt cgttatctaa agcaacataa cgtttagtcg
    44461 tgaaatcatt gatatcaaca taatcatttt tctttcccac agcgctttgt tcattgacat
    44521 cggttgttcc tcggatgatc cctttttcgt caacgacccg tatttcgatg atatcgctag
    44581 tcgcactgtt ttcaataaat cttttgatat tcgcatttac ctcttcatca tctgcattat
    44641 cttttcgatt caattccggg ctgattgttg ttgctaatga ttccaccgtt tgattcatat
    44701 ctttaataaa tgtattgatc gtggaacgtt ctaaaccacg tataaagtag gcaccaataa
    44761 tttcgatcga gatcaataaa atcagaataa aggtaatagc gatcttaaaa ttgactgatc
    44821 gaaaaaagcg gacttttctt ttcatccaat tactcctgtt ctgggttacg caaatagtaa
    44881 ccgacaccac gacgtgtcac taaataattt ggatggcttg gattatcttc gatcttttca
    44941 cgcaaacgac gtacagttac atccactgtt cgtacatctc caaaataatc ataaccccaa
    45001 accgtttgta ataaatgttc tcttgtcatc acttgtccaa ggtgcttcgc caagtaaaag
    45061 agtagttcga attcacgatg tgttaactcg attgtttctc ctcgtttcgt caccatgtaa
    45121 gcttctggat ggatcgtcaa atcaccaatc gtcaattccg caggtgctgc ctcttctggt
    45181 tccttcgcag ccgtggcacc gcgacgaaga tttgctttga cacgtgcaac taattctcgg
    45241 ttggaaaatg gcttggtcac gtaatcatcg gcgcctaact ctaagcctag gactttatca
    45301 atttcagaat ctttggctgt taccataatg atcggcatat cataagtttt gcgtacctca
    45361 cgagcaactt ctaagccgtc cattttaggt agcatcaaat caagtaaaat caaatcaggt
    45421 tctacctcag caactttttc caaggcttct tccccatcat atgcagtata aacgtcatag
    45481 ccttctttgg ctaaattgaa tttgacgata tccgaaatcg gcttctcatc atcgacaact
    45541 aaaacttttt tcataaagag ccacctctta tatattattg tcataccttc tcattatacc
    45601 gtatttctgc ttttgtttca tctttgccta taaaacaaaa gagaaaatta aaatgaaagt
    45661 gcaaaatact ttattatttt ttgttctaac ttcaataaaa aaggaaaaca ctgattttca
    45721 cctcagtgtt tccttttttt gatttatcct tctaaaactt gtacaccgac acccaataat
    45781 cccggaccag tgtggactac taatgccgga gaaatctggc caaagtagat ttggtttgct
    45841 tgtgggaact ctcttttaag tctcgcttcc atttcaattg cttcttcttt cgcatcccca
    45901 tgggcaacag ctaagttgaa gacctttgca ttaccgattt tttctttgac gtaagcaacc
    45961 gttttatcca aacttttttt acgtccccta gcttttccta ctgtgtaata aatcccttct
    46021 tcattacatg agatgattgg gttaagcttt aaagcggttc ctacaataga agcaactaaa
    46081 ccgatccgtc cacctttttg aagatattcc aatgtcgcaa cattaaagaa aacttttgtt
    46141 ctttcgacta actcattcag attactgatc agttgatccc attctactcc ttcttccagc
    46201 cattttgctg cttggatcgc agaaaaacct gcaccaatac caatattttt cgtatctagt
    46261 acatatgctt ccaattcttc ttgctgttgt gcaattaaac gaaccacatt atatgtcccg
    46321 ctcaatccac tggaaattgt taccgcaagt accttttcat aaccgtcttt tttgatttgc
    46381 tcaaagattt ctgtgatcga ctcaccatcc ggcaaagatg taccagggat ttcctctggc
    46441 aaacgttgat agatctcttc aggggtaatg tcaattttat ctgtatatac ctgatcttta
    46501 taaataattt tcaatgggat catatacatc tcgtattttt ttatcaattc ttctggaaca
    46561 tccgtacctg aatctactaa aattgcgatt ttattgtttt tcatttgttt tctcctcttt
    46621 taaatattcg gtttctagaa caatcgtttt ttccgcaaga agtttcattg caaacgataa
    46681 agttgccgct cttacagcaa tatattctac aggggttgcc tggtcgattt tttgcggatg
    46741 agatttacct tgtgctaatt ggctgaccat ccacacagct gcttcttgtt catcgcaaaa
    46801 tagattatag gcttcacgaa ttcctactgt ttttccttgg aacaatatcc cttctttgat
    46861 ttcaggaata gtcaatactt gtttcaacgt agtgattgca agtaaaaatg ctacgtgttc
    46921 tttgttatat ctttttttaa ctggtgctgg tatcaaacct agtttaacgt aattgtttac
    46981 catagaagag gtaagcaatg gatgcttttc tccttgtatc acaggtgaaa gataacgatc
    47041 aactaacgtt ctgacttggt ccatataaag ttctaagtca gggagttctt cccagtgagg
    47101 cagacggaat gtttctagtt ccttccccca ctccattaat tgctgacgca ttttttccat
    47161 cataaccgct ccttttttca tttcataaac atgatatcag acttatgata aacattcaat
    47221 ctagttgctt taactaggtg atttgattag cgggattcca tgaaaacaaa aaacaagaag
    47281 gctaagcctt cttgtctttt atcgttaaga acaggagacc cgccggggag acggcgggaa
    47341 aggagtaaaa aatgaaaaaa gtgttagggt ttgttctctt gcaagaactt tgtttgttgg
    47401 tatgcttata taatagcgag caaatgtgaa taatctatga atgatttcct ataaggaagt
    47461 aaagattttt ctgattttct ttaaatgtag tcttgcattt taaaattctt agtgctacta
    47521 ttattaagta ctttttgtac gggccgttag ctcagttggt agagcagctg actcttaatc
    47581 agcgggtcgc gggttcgagc ccctcacggc ccattgggtg ccaaacccac ggaacaggta
    47641 aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt ctttttttat ttttcaaaat
    47701 aactagggct ttttattttt aactcaaagt ccactgtagc cctttggact ttgagttaaa
    47761 aaagaagcat aattatgaac agtaaagttt ctaatataac aataattcga gaagctttgc
    47821 aaaatcatgc tgccaatgaa ctctctaaac atataggatt aaatctaagt actataaaag
    47881 gttaaaaagt gaagaatatt taatagaaaa actaaattta cttgctgctt tccccttttc
    47941 cattaataca acaatcagag agtgtctttt tttcgaaaaa attagaataa ataaaagaca
    48001 cgtagttttt ttatctacat gtcttccatc gactacaagt tatcatttct caaaacgtat
    48061 ttagctttta attgattaat cgtttcacgt tgtctacaca ttgcctaatt aagattttct
    48121 tattagaagc atcaatcccg caataatacc taaaactcca ggaattaccc acaataatgt
    48181 taaaagaaaa aatggaataa tagataaaat gagtaaacta ataccataga ccactgtttt
    48241 ttgatttttt cttactaaaa aacctattat gaaaatcatt atcgaaaaaa ctagggtaat
    48301 tcctccaaat aaaacaacat tcccgcttag attgactaac atatctatat ccttactggt
    48361 tagttctgta gtatttaaag tagttgaatc aatagaagat agaaacatac cacccatcaa
    48421 tgctaaaaga ctacataaaa tccctgttat tccactaatt aaaatcaaaa taaactctgt
    48481 ttttctctta ttcatttaat tctctccaga ttaagtgttt tagaattaga aattttttga
    48541 ccatagttaa tagcttctaa tgaacgaata acaaccactg acgtgttaga aaaataatcc
    48601 ccaatatgtt gtttcttggg cgtgaaagca gccaccaaat acccaatttg aaatacaaaa
    48661 ttttttaata taaacctaca tatacactca cgaataaaga tagtttgcca acttagctct
    48721 ttttcctcaa agctcactac tcttatccca aaaatcattt tcccaattgt ttgtcctgaa
    48781 ttcagcttag tcaaaagtac aaaataggtt aaataaatag ctaatgacgc tataccataa
    48841 aaagtaataa tggatttgtc agattccagt cccatgattt tgtaaaagag acctatagta
    48901 gcatgggtga tgatgctaat acaaattaaa tcaataagaa aagcaaaaaa tcgtaaccaa
    48961 aatcccgcaa atacataatt aggcaaagtt tcaatgccac tcttcttatt cattttatag
    49021 ccctccatag taatacattg gttttggagt atcagaaaat ccaagttttt caaaaactga
    49081 aattacacga tctgatgtag ttgcctttag cccttgccat tcagcaatct tattacctaa
    49141 ccaagaagaa gcaaagccag ttgtgtcatt tttatattct ataaccgtcg catcagttaa
    49201 tttcttctct ttttttaggg aatctaatgc atcttcagag taaccaattt gatccaccaa
    49261 cccattttct actgcttggt taccatcata aattcttcca tccgcaagtt ctttcactgc
    49321 attagtatcc atatttcttc cttttgctac aacattgaca aaacgatcat aagcactcat
    49381 cacaaattcc tgtataactt tattctcttc ctctgaaggt ttgtgttgag gtgacatcat
    49441 atcttttaat gcaccacttt tatacgtcgc atcagttacg ccaagttttt ctaataagcc
    49501 agaataattt aaaccagaaa taatgacacc aattgatcca gttgttgttt cttccgtagc
    49561 aaatatttta tctgaaccag cagaaatgta gtaccctcca cttgccgctg tatttttaaa
    49621 tgcagtatag ataggaatat ctagcttttt aatttttgcc atttctttag caatctcagc
    49681 actttcatat attcctcccc caggtgaatt tacttctaat aacactccct taaccgcttt
    49741 gtcttcttga attttcttta gctgtgttaa aaaattctga tggttatatt gttccctact
    49801 aaaaagattg ctttcacctg tatctgcaat aactccattg acggatagtt tgacaatctt
    49861 tttatttgat gcgccttctt ctaacgtttc ttctgtgatt tcgttagatc cgtaaaaaat
    49921 cttatttatt cctgttaggg ttgtatttga tgcttcctct ttatcttttc ctggaattag
    49981 cgcgacgata atggatagta acaataatcc gcacgctact cccacagcaa tccatcttct
    50041 tttattcata taaactcctc ctatattctt agtaaaaata attatttttt atttagaaac
    50101 acatcgacaa tttgttgcca atatgttttt tctcctacaa ttaaagacaa ctctccagtt
    50161 aatccataac gaaacggaaa cgattgagtt gtttttaacg ttcctttcac tgcataaaaa
    50221 acacctttct cagtggcagt agaattttct gaaatttcat ctaaagttcc gtcgatcgta
    50281 atcggagccc cacctttttg atccaattta aaatgaacct tcatcccttc tttaatatag
    50341 gtactttcat tcgcaggtaa aagtgctgta aatttcattt ttgatttttc gtggatcgga
    50401 taaatttctg caagcactgt tcctttggct agttctgctt gtccctttgt ttcttcattt
    50461 aaatgaaccg ttccagatac aggagctttt agtattcctt ttgaaagttg atcgtccaaa
    50521 cttttgataa ctgactggtt cttctcttgt ttttcattta gctctgtaac tgtttgtttt
    50581 acagttgtta ttgcttgttc tagctcttgc tttccttttt ccgtttgact aattctgtca
    50641 ttctcagaag tagttggttt aactaattta gctttttctg actgatactg ttctacttct
    50701 ttttttagtt gctcaatttg ttcatttatg cttgctgaaa ttgtcagttt cacttgattt
    50761 ttttgatcat cagaaacatt atttagttgt tcttgccaat tctcatagtt tgccatcatt
    50821 tcttttgaaa aatcttttaa tgattgatta ttactccaag ctacctgtac ttgttgccat
    50881 tcttgtaatt tactatctga actctcaatt tgtttttcta gttgtctttt tgtcttttcg
    50941 tatacttcta attgcttttg atcatttaat tcactttgct tcaaagcgta gcggagtgat
    51001 tctttttctg acaacatgct ctttaattga ttactgtagc caaaactgtc atccgttgaa
    51061 aacaaatttt ttcctttact tatactttca ataaatagct gagccatctt tatttgttga
    51121 gtaatacgtt ggttttcttg ttctatctga gccttttcat tctctattaa agaacaatcc
    51181 aatctaacca acacatcgtc cttatgaaca aattgatttt cttttaggaa gttttccatt
    51241 attttagctt ctaatggcac ttgtaacttc tctatttttt cggggttcaa ttgagccgtt
    51301 gtccgaataa caacctcttt tttagcaaag gttaaaaaca atccaataat acataaaaaa
    51361 agaattacag ggtataacac ccaacggtag aagctatgat gttgtttagt atagatacta
    51421 gaatgttcga accaattatt attctgcata aattgtttcc ctctcctaga tttcccataa
    51481 tttttgatag ataccatcgg caaaacgaag ttcatcgtgc gttccttttt ctaccaaatg
    51541 cccttcatgg agaactaaaa tttgatcaca tgcttttgct attggtaaat gatgcgcaat
    51601 aaaaataatc gttttttctt ttaaattgag gagataattc ataattgcat gttctaacaa
    51661 ggtatctaat ccactagttg cttcatctaa aattaatatg tccggatttt ttaataaagc
    51721 ccgtgctaag gctaatcgtt gtcgttgccc tcctgaaaga ttatttccac cttcttccaa
    51781 caatgtctcg taacgtaatg gctgttgatt aataaattca tttagttgga ctatctcaca
    51841 aacttctatt atttgttcga aacttggtgg gctatctaaa ccgaatataa gattctcaat
    51901 gattgttcca ctaaagaaaa atgactcttg aggcaaatat gtcactcgat cgcgtaatat
    51961 ctgaaaagga atgtctaagt gattaatgtt tccataatgg atcgatcctt ctgatgcttc
    52021 ataaaaacga acaaataact ttgcaagagt tgattttcca gaaccactaa ctccaactat
    52081 agccgtttta ctgtgcgaag gaattgtaca tgaaatttcg ttcaacgttg gggccttcat
    52141 gctatacgag aatgaaacgt tttttacagt gatatcttgt tgaaataatt gttcagaaag
    52201 catatattca aaagactggt tcgatttttc tgaatcaatc gcgaaaattt catttaaacg
    52261 attatttgca acttgagccg tttgcatttt cacttgtaaa ttaataatgt tttgtaaagg
    52321 atctgtcaaa aatactagta aagcattgta tgtgataagt tgacctagac taattctatt
    52381 atccataaca aaataagaac ctagccataa aattaatgca ctgctaatta attgaatcaa
    52441 atgtttaatt ccttgttgaa tattatcaag cgtaaccgta cgaaatgatt ttttcattaa
    52501 tttaacaaat tcatggtcta tacggttata tactttctct tcaccgctat atgctttaat
    52561 tgtttctatt cctttaagac tttctatgat actagaattt acagctgcac cggctgccat
    52621 ttcttccctg ttcgattttt catagctttt cacgaaagcg aatattgcaa ctacataaaa
    52681 aggcaaagag catagagtga ttaaaaacaa cgtactattc tgaatagcta acgtaacccc
    52741 aacaagcaac accataccaa tatctaaaaa tattgataac gtagcacttg ctaaagcatc
    52801 aataatctta ctggcatcaa gaaaacgaga aataatttcc cctgattttc tcgtagcaaa
    52861 aaaattcatt ggtaaagtta acacatgttt gaaatactga agcataattg ccatactcat
    52921 tcgctgtcct aaaataataa gaagataact acgactatat tcaaacaata cccgaaataa
    52981 atagacagcg atcaagccaa tggaaattat atttaaagtt gaacgtgctt gattagggat
    53041 gaaaaaatca agaatacctt gaaaataata tgagcttcct atgccaaaaa acgtaatgaa
    53101 aatcgatgct aaaacgatgt ggaatactaa tcctttttgt ttccaaatta taggcaaaaa
    53161 agaacttaat ccagctactt tttctttaat aggaatataa gtttctctag gcataggtaa
    53221 taaaagaata cctgtccatt ctttttgaaa cgcttcaatc gtttttctat tttttccatt
    53281 agcgggatct gcaatccata gtacgtttcc cttcgtttta taaattacaa cgtagtgcat
    53341 atagctgtta tcaaccaata tatgtgcaat taatggtaag ataagatctc tttctttcca
    53401 gatctcacta tctgcttgaa tagccataca atcaaaagac aatttttcaa atgtcttttt
    53461 caatccaaat gctgaagtac cctctaaatc agtcccagat aggtcccgta atttatgaat
    53521 cgggatatca gttttaaaat attttaaaat catcgaaagg caagcaacac cacaatcttt
    53581 ttcatcttgt tgctgcacaa aaggaaatga ttttatcact tatctaacac aactccttct
    53641 aaaaaatttt tatagaaaaa ttctcaatat agctttaata cagtgaaaat attgtctcat
    53701 aattccacaa aaactttgaa aacagttctt gaacactaac tttttcattc ctgaatcaaa
    53761 tttattttgg aaattttact attcaagaat aaacttgata agtttcagga agatttttac
    53821 tgttaatcaa atacgtatgt tacagtagca ccgtaaaaaa tattatttaa agaagcgtgt
    53881 ttaaacatgc aaaatgtaaa ggaactaagt attgtggaaa tgcacaaact gttggtggag
    53941 gcaataatgc ttggggaaaa ttggggcaag ttgtaggtgg acttaccaca ggggcagtag
    54001 gtggtgcagg tttaggaaca gcaatttgcg gaccagcttg cggtgttgtt ggagggttat
    54061 atggagctgt cgctggaggt gctgcagctg gctgggatgc tcgaaaaaaa taaaaaagga
    54121 gaataagtag tatgcatatt aaaaacacaa aaacaacatt tatattatct tcagaagagt
    54181 tgaaaaatat tcaaggtggg agtgccgtgg gagttttagg cactactttt tctggggcaa
    54241 cagcaggagt aaaactttgt tcagccggag gaccttatgc aatagctgct tgtggtgtag
    54301 gtggagcttt attaggtgct ggattcagca tgtggaccgg tgcgtaaaca attaaaaaat
    54361 atcaaacctc aagattggtt tgtgtcgatt acgaacatag gtatgttctt tatcttttca
    54421 aaagtttgga atggacagaa ctttttacta aagcttcttg tgctagtttt tataattata
    54481 ttaaattttt atcttttatt aaagtcagat aaaaattctt tggaaatgta atggtagaga
    54541 tatttttaca aaaggacttg cagcgctgta gttggtgctg caggaggacc tatttcggcc
    54601 gttacaggag gtattggtaa tgtacttgtc ggatgtgcat taaagcctgt tcctgcaagt
    54661 taaatttata aaaacaggaa agtaattatc tcaaatccta tcaagtacaa tcataggatt
    54721 gcacttgaca ggacaaattt tttaactaaa aaatgaaaaa cactttctaa aaattagtag
    54781 aatttgccaa atctttcgca tcttccacca tacatttaat tttaattccc ctttatggta
    54841 cttaataaaa acaagaaaag aactattcat ttggcccttt tttggttcca gaccatagaa
    54901 atattcctcc agtaactaat attgctgtta tgcacgtaac taataacagc tataaaaatt
    54961 ggtacaactt taataggatt cagaaaaatt tgcactagtg aaggaatcta ttttattaga
    55021 aaaatgcaat ctccctaaag aaaaaaccac taaacagtgc tttctttcaa cttctcctta
    55081 attttttgtg ctttccctaa agtaaagttt ctggccaact ctgaatcaaa atatagtgaa
    55141 cgttttttaa aatctatttc tttaacgtta aagatattta ctaaacacgc tcgactaatt
    55201 cttgttaaca taggatattt cttttcaagc tcactaattc ttccataaaa ttcgtactgc
    55261 ccatctttag tacacaatga taatctatgt ggtaattttg aagcttctac aaaataaatg
    55321 tcatttatat caaaagtaaa tgtttgagaa cctatggaaa aaacaaatgc ttgattcgta
    55381 acctgttttg ttgcatctat cctttcttgc gctaataaca ataactcaac aatctcgttt
    55441 cgatactcat ctagtgtttg atcttttgtt acaaatccta acgtttcaac tcttcttttt
    55501 attgttacgg gtaacatctc atcatgagta gttgtaaaaa tgatttttgc ttgaacatca
    55561 tattttctga ttacttctgc taattctata ccgttaactt catgatttaa atcaatatct
    55621 aagaaatata ttccttgctt tggtcggaat tgtttgaggt attttttaac ttctaacgga
    55681 ctttgtgttt tcaatactat cttaaatact ttatcgtgaa aaagaatgaa attatcaata
    55741 attcgttcta actgatttaa ttgtataatt tgatcttcac ataatattat tggataactc
    55801 ataaatcctc cttccctcaa aacaacaaaa taacttgagt agtaaattga tacggatcgt
    55861 tttgatagtt cacaaacata ttgggattct ttttattgat ttcctctata ctagataaac
    55921 ctaacccttg atgtcccttt tttgttgtta ttcctttttt cattagagta tctatagaga
    55981 gtagtatctc tctacaacta ttcgatatcg aaatttctat ctgtttatca tctcggtaaa
    56041 tagctagaga cagttctcta tcttgactct ctatagatgc ttcaattgca ttatcgagaa
    56101 taattccaag caccctaata cagtcaaata ttggaacagg aacttcaata atagtacttg
    56161 gacattcgaa tttacaacaa atcttattgt tatttgcttg gtatagcttc gctatcaaca
    56221 aacttttcaa atattcattt tttatatttt ttaaataacg atattcaaac ttattggtta
    56281 gataagaatt agaatagatc tctaattctt ttatttcttt ttccaatatc gaatcatggt
    56341 tttgattagc gatttctttt aaactcagca ataaattttt atagtcatgt ctaaatctgg
    56401 caaatttttc ttgatctttt tccagtgaat cagtatattt ttttagataa accaattctt
    56461 ttcttttcaa ttgctgttca tattgttctc gttgcctaat agttattcta ataaataaaa
    56521 atagaacaac aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa
    56581 atacttgata ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca
    56641 tgaatatagc agtaagttta gaactggact gttcaacaat cctatttata gctaattttc
    56701 tatataacca aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat
    56761 aaccataccc ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaataa
    56821 tagatctgaa atcatcaata agcatacata atagtaattt atttaaaagc actatattag
    56881 ttttcttctt ttcctttaat ataaaataac ttccgattat aggtatagct gcggaaacat
    56941 cagtaaataa ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa agatattttc
    57001 ttttgttaag atcagtatta gtcatataaa ataagagaaa aaagaaaaag tttcccacga
    57061 acattcttaa tagataagct tttgtatcga tagtaatcaa gagatcccct cctgatttta
    57121 ggatatcata aaaaaaattt tagttacact ttttaaataa tgaaaaaaca cattccacta
    57181 aatttgatgc cggttttcct tgaggttttg ttccagcacc tcctttaatt tttttaagct
    57241 cttgatctga acattgttta gcatttagtc tatttttttc ttccattacg attcccccta
    57301 caaactataa atacttttat gtcactgatt caatatacct atttttctaa tcaaaaaatt
    57361 ttgagacata tttaatatta taagtatcat taaaattgag atttatctcc ataatctgct
    57421 cgatataaac catttattcc agcagttctt ccaatttcac tcaaatttct tattaattcc
    57481 tcttgatatt cagtgaatct tattttatta gtataagcag taaaatatag ataattcacc
    57541 aatctattta ttaaaggacc gggatctaat tcatattttt gtttttctaa ttttaaatat
    57601 accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca
    57661 ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat
    57721 tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca
    57781 atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt ttccactatg
    57841 agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat
    57901 aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca
    57961 aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt
    58021 taagtatata aactatgtat attctttaat attttttgag aatgcacttc gtacataata
    58081 aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa
    58141 ttcataatag tttgtcggcg tctttggacg tttcgaagat gagctacgca ttctcttttt
    58201 ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat
    58261 tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc
    58321 ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc
    58381 caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc
    58441 ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc
    58501 cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg
    58561 tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt
    58621 cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat
    58681 cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc
    58741 tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc
    58801 aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata
    58861 ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga
    58921 aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg
    58981 tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga
    59041 taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa
    59101 gatcactact actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt
    59161 tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat
    59221 gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg
    59281 caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac
    59341 cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa
    59401 aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc
    59461 tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag
    59521 agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc
    59581 tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat
    59641 actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga
    59701 gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg
    59761 gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga
    59821 aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag
    59881 taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc
    59941 agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg
    60001 atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca
    60061 cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg
    60121 gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca
    60181 cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat
    60241 ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt
    60301 cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca
    60361 atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa
    60421 attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca
    60481 tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat
    60541 catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt
    60601 gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct
    60661 caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact
    60721 gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt
    60781 tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca
    60841 agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac
    60901 ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac
    60961 tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc
    61021 cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca
    61081 caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca
    61141 tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt
    61201 ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca
    61261 cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac
    61321 tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta
    61381 atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct
    61441 gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg
    61501 ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg
    61561 cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag
    61621 ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct
    61681 gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga
    61741 tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg
    61801 ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat
    61861 ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa
    61921 gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg
    61981 ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt
    62041 tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa
    62101 aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg
    62161 ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg
    62221 cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct
    62281 tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa
    62341 acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc
    62401 aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag
    62461 cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc
    62521 gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta
    62581 tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca
    62641 aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg
    62701 actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg
    62761 aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag
    62821 ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac
    62881 ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc
    62941 aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt
    63001 ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga
    63061 tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg
    63121 aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc
    63181 atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa
    63241 agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag
    63301 tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact
    63361 gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc
    63421 cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg
    63481 atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc
    63541 acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca
    63601 ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata
    63661 aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt
    63721 aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt
    63781 accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct
    63841 cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga
    63901 acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt
    63961 ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt
    64021 tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc
    64081 ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta
    64141 gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc
    64201 acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg
    64261 ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct
    64321 acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt
    64381 ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg
    64441 actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg
    64501 ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct
    64561 tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat
    64621 caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta
    64681 tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata
    64741 aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg
    64801 ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta
    64861 aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata
    64921 tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc
    64981 tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata
    65041 gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt
    65101 tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta
    65161 ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt
    65221 ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa
    65281 aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa
    65341 atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga
    65401 aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat
    65461 tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga
    65521 tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt
    65581 tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag
    65641 gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa
    65701 tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt
    65761 tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta ttatggaaaa
    65821 aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc
    65881 atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt
    65941 tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta
    66001 tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga
    66061 agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc
    66121 ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca
    66181 agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg
    66241 tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa
    66301 aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt
    66361 cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt
    66421 ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg
    66481 cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa
    66541 aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt
    66601 ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa
    66661 acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg
    66721 ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat
    66781 catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg
    66841 caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct cgacaaatat
    66901 cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt
    66961 gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa
    67021 gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca
    67081 aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa
    67141 ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa
    67201 ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt
    67261 ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga
    67321 agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt
    67381 ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc
    67441 tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc
    67501 gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg
    67561 taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca tgagtttgac
    67621 tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca
    67681 aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat
    67741 ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag
    67801 ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa aggagcattc
    67861 tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat atcattgata
    67921 aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa
    67981 atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat
    68041 ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta
    68101 aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg
    68161 ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag
    68221 aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg
    68281 aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat
    68341 tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta
    68401 acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg
    68461 gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag
    68521 actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat
    68581 tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca
    68641 aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc
    68701 caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg
    68761 gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat
    68821 tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac
    68881 agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac
    68941 ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg
    69001 gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa
    69061 tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt
    69121 cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa
    69181 aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc
    69241 tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac
    69301 cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc
    69361 aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc
    69421 ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc
    69481 aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa
    69541 aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa
    69601 tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt
    69661 gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc
    69721 atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt
    69781 catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac
    69841 tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa
    69901 accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg gactaaaaat
    69961 attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca
    70021 gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat
    70081 acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa
    70141 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
    70201 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
    70261 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
    70321 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
    70381 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
    70441 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
    70501 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
    70561 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
    70621 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
    70681 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
    70741 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
    70801 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
    70861 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
    70921 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
    70981 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
    71041 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
    71101 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
    71161 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
    71221 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
    71281 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
    71341 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
    71401 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
    71461 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
    71521 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
    71581 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
    71641 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
    71701 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
    71761 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
    71821 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
    71881 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
    71941 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
    72001 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
    72061 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
    72121 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
    72181 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
    72241 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
    72301 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
    72361 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
    72421 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
    72481 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
    72541 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
    72601 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
    72661 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
    72721 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
    72781 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
    72841 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
    72901 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
    72961 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
    73021 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
    73081 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
    73141 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
    73201 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
    73261 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
    73321 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
    73381 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
    73441 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
    73501 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
    73561 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
    73621 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
    73681 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
    73741 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
    73801 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
    73861 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    73921 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
    73981 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
    74041 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
    74101 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
    74161 aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccttgtactc gtgtttgatt
    74221 ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa gcaaaatggc
    74281 caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa caagtcccat
    74341 atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag gtgcagcaac
    74401 tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc aattgtaggc
    74461 gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc caaacatgaa
    74521 aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat gcggcaaact
    74581 tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac gcggcatttt
    74641 ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat agatcatccg
    74701 ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa tccccacgag
    74761 taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg ctagtaccgc
    74821 accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa aagaagcgca
    74881 tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg cagataacgc
    74941 tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa ctaaaaacag
    75001 taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg cacttgtgct
    75061 aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca tattggcaac
    75121 aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag gttttttttc
    75181 attactcttc attgatttcc cccatttcct atcttagttt acaacttatg gaaaagattg
    75241 gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt ttccttgttt
    75301 tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa cacttgcaac
    75361 caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac acgcatacaa
    75421 cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta gaggcaattt
    75481 catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca tatagcctaa
    75541 cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat gattcgtttc
    75601 ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga tgttaccaag
    75661 agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca aaccagcaat
    75721 gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg tcaatacgat
    75781 caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct ctgccttact
    75841 cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca accgatagcc
    75901 cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta ttttcttttt
    75961 catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat aaaaacaaaa
    76021 aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt atttaacagt
    76081 tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt ctttagatac
    76141 gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt tcaagcctaa
    76201 accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag ctgcagttaa
    76261 ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag cagctacagc
    76321 tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta ggtcgttaag
    76381 ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca atgccattat
    76441 aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat taagctgcat
    76501 ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg actggcgctt
    76561 gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt gccaatgctg
    76621 tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt tctaatgctt
    76681 ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct tcgttactga
    76741 atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca gcttttttag
    76801 ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca tcacgaagtt
    76861 gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg actactactg
    76921 atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt tttgcgataa
    76981 ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt ttttgcactg
    77041 aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc acctattcac
    77101 ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc tttgctcaac
    77161 cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa aactctatgc
    77221 caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt aggaaattaa
    77281 ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac tagcttagca
    77341 tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat tctaatattt
    77401 aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt gatcgttaca
    77461 tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac atcatggatt
    77521 gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat tggtacatgg
    77581 atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc gtttactgct
    77641 tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc ttttggtcct
    77701 aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac aactacatca
    77761 aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac gaaatctgca
    77821 ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac agtttgtgtt
    77881 ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc ttttttaggg
    77941 tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat gtttgtttct
    78001 tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac tttttttaat
    78061 gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt gtggttataa
    78121 cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag aagctgctgt
    78181 cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc cttcaaccat
    78241 gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt cagcgatttc
    78301 tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc ctgaaccttt
    78361 ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg taacgaatgt
    78421 gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg cttgatcagc
    78481 tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt gacctagtgc
    78541 aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt taacgatttt
    78601 ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt aattggagat
    78661 gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat aatgtgcaaa
    78721 acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt ttttctgcta
    78781 tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca atttttcgtt
    78841 ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga aagcaaacaa
    78901 taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga ggtggagaaa
    78961 atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat gattggtcca
    79021 agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag tatatttggc
    79081 gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac ctatcggggg
    79141 cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga tgatgaacgt
    79201 ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt tgttcctaag
    79261 agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg aacacgaaag
    79321 ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga attgaatgga
    79381 ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca agatgttcca
    79441 ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat cggcactatg
    79501 acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt agatgatcct
    79561 caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag cgtaaattat
    79621 ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga tcaagcaaaa
    79681 gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag tggccgttcc
    79741 cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca atcaatcaac
    79801 gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc aaaaaaatta
    79861 gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa agctgtacgc
    79921 aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc tacaccgact
    79981 gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg gcttgatttg
    80041 acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt agtcatcgca
    80101 aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg ttctgcaagt
    80161 gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca agcagcacaa
    80221 gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt tgcaggctta
    80281 gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt tatctctgct
    80341 gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt tgctgcaatg
    80401 taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg tggcttagct
    80461 gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc gtgataaagc
    80521 tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga agagaggact
    80581 aattgccctt ctcttcgttt tttattaatc catcaatttc gtatttttta cttcatcgat
    80641 cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt gttcaaaaac
    80701 agatgttact ccttctgccc cacctagatt caacccataa atcactggtc ggccaatcgc
    80761 aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac ggacaccaga
    80821 gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca atacttcaaa
    80881 ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga taccatctgc
    80941 ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta cgataactgg
    81001 aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt gttttgcagc
    81061 tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt gttcgctata
    81121 cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt cttcttcacg
    81181 gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg cttctactgc
    81241 tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt ggaaaaactg
    81301 cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat aagtgctgat
    81361 ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc ctttttcatg
    81421 tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt caattccgaa
    81481 aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca tgattttctt
    81541 tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac caccgcgaat
    81601 atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt aggcaatgtt
    81661 tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag atccttcttt
    81721 catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa acatttactc
    81781 tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga tctacttctt
    81841 ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt tcctctattt
    81901 catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac ggtactgttc
    81961 catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga gtggttatag
    82021 gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca gcaggcccct
    82081 ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc
    82141 caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac gttcctaaat
    82201 agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta tctatgccgt
    82261 agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc cactcgttat
    82321 ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca gacaaatctc
    82381 ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt actgcttgct
    82441 gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg ctgtagccgt
    82501 ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct gccgtttttt
    82561 ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca
    82621 aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg attggttctg
    82681 tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca atcatatgat
    82741 cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc aagttctgta
    82801 ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc tccgtcaact
    82861 tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac gacttcgcca
    82921 acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa gatatgattg
    82981 acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc aacgaatcct
    83041 gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat catttcaaca
    83101 agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt tttgacttca
    83161 gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt catactttgc
    83221 gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt atgcaagaca
    83281 taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa
    83341 cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta cgttgtggct
    83401 agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca ttacgtaaaa
    83461 catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac ggagttgttt
    83521 tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa atttcatgag
    83581 acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg attacaatat
    83641 ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac tgattttgtg
    83701 tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc catttacttc
    83761 tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac tttttacttg
    83821 tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga ttaatttgaa
    83881 attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac ttgtatataa
    83941 gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat taaaaatttt
    84001 agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata ttttccgtgg
    84061 aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata tgacgcttcc
    84121 tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt ctttcttcaa
    84181 agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg aaaatcattt
    84241 ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt ggagcgtaga
    84301 acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt tggctgctat
    84361 tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg atatcgaaaa
    84421 agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag cgaaagtatg
    84481 tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt
    84541 gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct gctttctttt
    84601 ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa ataatacatc
    84661 aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc ataggttgtc
    84721 caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat gctgatggta
    84781 aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt ttgcaataaa
    84841 gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc catgatacca
    84901 aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag caatttacgg
    84961 ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg ccctgaggat
    85021 acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc atttacaacg
    85081 attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct tttctcctag
    85141 tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa taattataac
    85201 agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa
    85261 tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg ggtcataaga
    85321 attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg aaacttaaat
    85381 aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc tagcaagcag
    85441 ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag taatggatca
    85501 tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt ttcctagact
    85561 tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa caagtttctt
    85621 tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca ggagtattaa
    85681 aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc atatattgat
    85741 caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt taccggtatt
    85801 aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac acgatgctga
    85861 gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag aacagaaaac
    85921 aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac atcaggtact
    85981 tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag caggttctgg
    86041 aaatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac ttcagctgat
    86101 tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa aaataagtga
    86161 aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta tcgtccatgc
    86221 gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat cattttcgta
    86281 tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc tggtagatgc
    86341 aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga agcatccttt
    86401 ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag tcattgattt
    86461 tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt tacttataca
    86521 gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat tacgtatcgt
    86581 ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac gaccttttga
    86641 tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa
    86701 gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt ttgtcacagt
    86761 ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat agtttctttc
    86821 tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta agacacaacc
    86881 attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac tgatcaagtt
    86941 attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa ccatttctgg
    87001 caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga taaaaatatc
    87061 tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag ccgaaaagct
    87121 aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg ttatatccca
    87181 cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca acagcagcga
    87241 gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa tgggcgcact
    87301 ctggtttcgt ttactgatct ctttgcgatt aaacgctaga ttgatgacgc tgaaaccgct
    87361 aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg tcaacagctt
    87421 tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg agagtgccat
    87481 ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca gaatcagtgt
    87541 ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat ctgggcgact
    87601 ctgtatcttg atcgtcgcca tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag
    87661 atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt tttgtgaggt
    87721 tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc catcgactac
    87781 aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg tattttcaac
    87841 atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac ctatcagtaa
    87901 attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca taacgcaccc
    87961 ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa
    88021 gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa ctttcgctga
    88081 tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc caatacttca
    88141 cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact gaaatcaaga
    88201 tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat gtacctaaca
    88261 aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa cgttcaccaa
    88321 agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct gtcaaaacaa
    88381 tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
    88441 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
    88501 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
    88561 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
    88621 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
    88681 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
    88741 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
    88801 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
    88861 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
    88921 caattccagt attaatcgtt tcaagattgc caccttgatc aatctcgcca aaggccgcca
    88981 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
    89041 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
    89101 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
    89161 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
    89221 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
    89281 gctattgttc gctgaatcgc tgacaacaca ttttctggga gtaatgggat gtcgatcgtc
    89341 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
    89401 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
    89461 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
    89521 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
    89581 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
    89641 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
    89701 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
    89761 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
    89821 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
    89881 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggacctaa cagacctaag
    89941 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
    90001 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
    90061 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
    90121 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
    90181 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
    90241 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
    90301 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
    90361 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
    90421 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
    90481 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
    90541 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    90601 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    90661 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    90721 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    90781 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    90841 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    90901 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    90961 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    91021 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    91081 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    91141 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    91201 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgatgatga tccatttgtg
    91261 acgaaatcgt tacaaaccat tttagaaagc acgaaagaga tccgtgtgtt aggcattggt
    91321 cattgtgcca aggatgcgct agtcttatac gaaacccatc gtcccgatgt tttgctgacg
    91381 gacatccgca tgcctaaaca aacgggcatc gatgctgcca aagaaatttt ggctaaattt
    91441 cccaaagcca tccttttgct tttgacgaca ttcaaagatg aagaatacat ccgcgaagca
    91501 ttttccatcg gtgtcaaagg gtatttaatc aaacagaact tgcaagcgat tattccttct
    91561 gtcaaatccg cttataatgg acaggccgta tttggtaatg aaatcatcga gactttcact
    91621 caaatgatga agaataaacc gacgattgat agtcatgctt atgcttcttt tagtgaacgt
    91681 gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca ataaagaaat tgcagatgcc
    91741 ttgtacttga gtgacggcac tgttcgcaat tacatcagcc aattactaga aaagctagat
    91801 ttacgggatc gcacgcaatt ggctatctat ttttatcagc atttgcaata agtcttatct
    91861 aacacaaaaa aacctgaatg atgctccttc gcatcattca ggtttttttc acatcttcta
    91921 ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa tcaaggattc gcagggctgc
    91981 caggattcgt tggattgttc ggcgtgttag gcggaaaaat aggcgccttg tctaacgttt
    92041 tttctagttt tgattcttca cgatcgtctc gttctacatc aggactttca ttcaatttga
    92101 attctttttc catctcatct aactttttaa agtcattgcc agattctttt gctaatttcg
    92161 ctaatttatc ttcggtttct ttagccataa agtaacgccc cccttttgat ttcagcttag
    92221 catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg atcattttta gctatttctc
    92281 atctttaaaa tcaagcattt tttgccggta taaaatcgta gggagaactg ttttccatac
    92341 caatcatcgg atctactaac tggttatcga tcacttctgc tgtaagaacg aaatctttag
    92401 aaacagcagg ttctacctct tctttctttt cttctccaac aaatgaagaa aacagatctg
    92461 gttccactgt ttccattact tggtttttcg tttcattcgt cccactgtca tcttctacag
    92521 gtagcttttc ttttacaacc cgtggctctt ttgtatagtt cccatcttca aatggatcat
    92581 ttccgaccaa agaatcttca taagctgcta gtttggtccg gagagtgata ttcattttat
    92641 cattatccat gatccttgat ttcaacgtag tcatccggct tgctgattca ttcgctacac
    92701 aagtgacata ggcttgctct tcccctaaca aaatcaacag ttccttactg cgagtcactg
    92761 ccgtgtagag caaattacgc tgaagcatcc gctgatattg atggaccatg ggcaaaataa
    92821 ccatcttgaa ttcactgcct tgtgatttat ggattgagca acaataagaa agagtgattt
    92881 tattccattc gtttctttta taagtgacct catttgcatc aaactggata actaattcat
    92941 ccactttatc ttcagaatct tttgccaaga tgatacccgt gatttctccc atatctccat
    93001 tgaatacatt caattcggga gtattgacta attggaggac tttgtctcct atccgataaa
    93061 cggtgtcgtt ccaatgtact tccttttttc caggtttccc tggattgaat atctcctgca
    93121 tcattttatt taacgcatcg atccctgcag gtccccgata cattggtgct aatacttgga
    93181 tatcttgtgc acggaatccc ttatttttcg ctttttccac aattttagag atcaagggtt
    93241 caatttgata agcatcactt cggaaatagg aacgatcttt ttgattcttt gtgaaatctt
    93301 ttggcagacg tccttctttg atctcatggg caagaggaat aatactagaa ccatcacctt
    93361 gccgataaat ctgattgagt tccattttgg gaatttgttc gatttccaaa agatcatgga
    93421 gtacttgacc aggaccgaca gagggaagct gatctttgtc tcctactaag ataacttgca
    93481 tattcgtcgg aatcgctttg aataatgtgt tagccagcca tgtatcgacc atagacatct
    93541 catcaacaat caaaagtccg ccttctaatt ctttcgtact gactgaaggt gttttttccc
    93601 gtccattcag ccccagaaga cgatggatcg tactggcagg cagacctgtc gtttcgttca
    93661 ttcgtttagc tgctcggcca gttggtgctg caagtagaat aggaaaaatt tcttgggtat
    93721 attttgaagg atccaattcc agtccgttca actctgcaaa aagattgacg attccgttga
    93781 tcacagtagt tttccccgta cctggtcctc ctgttaaaat aaatagaggg gaacggattg
    93841 cttcagcgat agcttgttct tgcgaaggtc catactgtat gccgaatatt ttttcgatac
    93901 gccgaatatt cttctgcact tctttttcag gataatggat ttctttttta cgactgagaa
    93961 ggcgctggat cgaattaccg atcccccatt cagaaaagta caaactgttt tcgtaaagat
    94021 tagtctcttc ttgttgtatc tttccttctt caacaagttg gataattaca gtagcaacct
    94081 gatcaggctg gatttcaatt gggcgacttg tttctaaaat acggatcgtt tgctgaagca
    94141 atgtctccgc cggtacgtat gtatctcctg tttgaactgc atgctgaaaa atctgatgca
    94201 agatagctgc tcgaattctt ctcggtgaat ctgctgcaat accgatttgt tcggctagat
    94261 tatctgcacg tttgaacccg attccttcta tgtcttcgac tagctggtac ggattttctt
    94321 cgatgatatc caaagactcg ttcttatagg cctggtagat actgaaagct aactgactac
    94381 cgaacccgta acgattcagt cccatgatca cttgatccat cccgtggttt agccgaatcg
    94441 tctcagcaag catttctctt tttggcttcg tcaaaccgga gatttgtttt aataccgaag
    94501 gatcagcaat gattttttcg atagcatctt ctcccagcaa ttctacaatt ttttctgctg
    94561 ttttcttccc gattcctggg aatttttcgc tggaaagata cgctacgatc ccatttgcag
    94621 ttgttggctt ttcttgctca tatctttcga gtttaagctg ccttccgtat ttaggatgat
    94681 ccactaattc tccataaaaa cgataagtct cattttcttg aatatcgccg aaacttccgg
    94741 tgactacaat ctctttttca cggtaatcag ctgtggtttc actgatgtgt accaaaagca
    94801 ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc aactgtacca acgaaataag
    94861 gcatttcttc tggctgcatc ggaagccgcc tccttcttct atagtaaagg ttctggtgct
    94921 gtttcagttc ctaaaaaatc tgtttggttc catactttca gttcatatgg caataatctg
    94981 caaggctctt cagtctctaa aatcgtcaaa ctactattga acaatccacc catttcacgc
    95041 aactcactta gctcttttcc tgtcatccat tgaatggctg cagttaaaga agctccatgt
    95101 cctacgaata aaagtgggcc atctttatgt cgggcaaccg catctgctac aacagtttcg
    95161 atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag gagaaggatc atacagatct
    95221 aaatgattac gcaaattcgg cagattatcc ggatatttct ggtacatttc atcgatcgac
    95281 tgaccttcta aagtacccag ccctagttct cttagcttat ctgtataaaa aatctctacc
    95341 ggacgagcaa gctctttgtt gataccttct gctgtatcac gtgctcgctt tgctgtactg
    95401 gaataaattg ctttaaaagg aacatatttt acctttttcc caaatgcttt gatttcttcg
    95461 taactttgtg gcaaaagcgg agaatcgcca ttgctccctt gaaaacgtct ttcctgattc
    95521 cattgtgtct tcccatgtcg ggtaaaatat agttccatac gcagcaaact cctgtctttc
    95581 ttttatttta ctctataata aactgtctaa tccagcttgt cattttcata gtgatgcgtc
    95641 aattctaaat tagggaatcc ttgctggcga agtgcttcat acactatcat tgcagctgta
    95701 ttcgataaat tcaatgagcg gacatgctca tcattcatag ggatgcgtaa acacttttct
    95761 tcgttttcac gcataaatgt ttctggcaaa ccggttgttt cttttccaaa taaaaagaag
    95821 tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa ttttgagatc
    95881 aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact gtggtaagta
    95941 atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc atcagttgaa
    96001 aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt acgggcaata
    96061 tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat caggatcatt
    96121 cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat aaaaaaaaac
    96181 gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt ctcactaact
    96241 tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc taatataaca
    96301 ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac gttttctctt
    96361 agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct tatataagtc
    96421 tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa gatctttttg
    96481 attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta atttcattgc
    96541 ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag gttgattcgg
    96601 ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct tcaacacttc
    96661 tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa agatatttaa
    96721 aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta cacaccaaaa
    96781 tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat caaatccaac
    96841 agcattttga atctttttca attttgaaag ttcattcaag aagctacctt ctccgcagcc
    96901 aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa taacgggctg
    96961 atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat cgtattctgt
    97021 tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa aacggtgatt
    97081 atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa ccggacagcg
    97141 gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt ttaacatagt
    97201 tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact gctaaaaata
    97261 tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat caaagaattt
    97321 tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga tcggatcgaa
    97381 ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat cgaagaaacg
    97441 ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc tagagggggc
    97501 gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat tgaagcaaag
    97561 aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg gctagacgaa
    97621 gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt ccatatggct
    97681 tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt agaacatggt
    97741 gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga aaatttccca
    97801 cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc tggtggtggt
    97861 gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga agtacacgga
    97921 accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg caacaggcat
    97981 aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat aaagaagcaa
    98041 tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt cgtgataaag ggaccgcttg
    98101 ctactgctgg atcaaatcct agacgatcca ttagcatcgg gattaaacta cctgcaagat
    98161 tcgccactgt gattgcaaat agcatagcca tccctatgac aaaccccaag ataaaattat
    98221 gtttccaaaa acctacgatt agaaaaatcg tgatccccgt aacagctcct gtgacaacgc
    98281 cggtcaagac ttcagccaaa acaagtcgaa caaatccatg atctttctca tcagaaatcg
    98341 caagtcgacg aacagccaca gctagcgact gggttccagc attaccggca gttcctgtaa
    98401 tcagcgaaat gaagactgct aaaatactcg cttcacttac caagtcttca tagtgactga
    98461 ttaaagaagc ggtcgacata cctaaaaaca acagagtgat cagccaaggc aaacgtcgag
    98521 acgctgcctt tactggattt tcattaattt cttccacatt gaccccggca agtcctgaat
    98581 aatcgctagc agcttcatca tcaataacgt cgatgatatc atcgactgtg acgattccta
    98641 acaaatgatc atcataatcg gtaacaggaa gagctaagaa gtcataatct ctaaatgttt
    98701 gtgccacatc ttcttggtcg tcgcctacat gaactgaaag cactcgttcg ctcatcagat
    98761 cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg cagagaaata acccctacta
    98821 gctgaccttc ttgattaacg acatagatgt aatagattgt ttcagctaca tctgcttcgt
    98881 tcttcagcac gtacatcgca gagcgaacag tttgattggc tacgatcgaa acaaattcag
    98941 ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag aagttccttg atttcactgg
    99001 catcgtctgc cgacatcaaa cttaaatatt tagcaatttg ctttttatcc agcgtattaa
    99061 gcaagtcaac agcattatct gtatacatct cagaaagcat atcagctgca tagctaggac
    99121 gcatttcatc taggtattct ttcatatgtt cgttgtcttc ttcaatcaca tcgaacatat
    99181 ccgctaattc tttaggcgac agataggaat agatcagctg cctgttctct tctgtaagag
    99241 attggtagaa ttgcccctgc tcatagatat gcatctctaa aaaactgttt ctgaatgcct
    99301 gaatattatt ttcccttaaa tcttgagtca actgggcaaa tctttcctct aattcttgat
    99361 tttcatccaa accaatctca cctcgcctta ttcttcaaac cgtctcacaa ctgcctccat
    99421 gtctgcggct aaaggctgct tgaactccag caactcttct gtaaatggat ggtaaaaacg
    99481 cagatcataa caatgaaggg cttgtctttt tattcccaga tccattcgcc cgccatacat
    99541 atcatcaccg agcagggcac agccaattgc ggaaaaatga actcttattt gatgtgtcct
    99601 tcctgtatgc agctggatat caactaacgc gatttctgtt agtcgcttct ctatccagta
    99661 ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc ttcaatagcg aagaataatc
    99721 tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa tctgaaagcc ttccactgac
    99781 taaggcctga tattttttca caaacttctt ttcacgaagt tgcatatcta attttgcgtg
    99841 cgcaaatcca tgctttgcaa aaagcatcaa gccagatgta tcccgatcta gtcgcgtcac
    99901 cacatggatt acttggtttt cgtaaccttg ttttttataa taagccttta cacgattagc
    99961 cattgtccca tttggatggt attgggcagg aatagacgaa acgcctgctg gtttatttac
   100021 taccagaacg tgttcatctt catacacgat atcgatcggt gtttcatcca aaagtacggt
   100081 ttcatgttct ccttcagcag gaatcacaat ggttacctta tcatccttag ctagagaaaa
   100141 caagacattt tctacttgat cattaacttt gatttgaccg ccttgaaatt ttattttcgc
   100201 taacagaccc tttgagatcc ctttttcttt taaaaaatat tttacttgct gagaatgatc
   100261 tttctcataa tcccatgtaa actgcatatc aatcctcact aataaaggca tctttcacac
   100321 gatgccaaaa atgcatatgt cgataagaag caaaatggat ccgttcttct gctattcgat
   100381 aataaattga gcggatattt gcttttgcga cattcaattg atcaaccgtt acaagataat
   100441 cttctgtatt ttccaatttt atttctagcc attcatcatg ggcaatcacg atcggagaac
   100501 ccaaagtacg aaacactcga ttattcagtg aagcaatctc tgccaactga aaggcattga
   100561 tactaggatg gatcacagca ccgccgacac ttttattata tgctgtcgaa ccagtaggcg
   100621 tagaaatcgt caatccatct cctctaaaac tttcaaacag ctcgtttttg atgtatacat
   100681 cggctaccat cgtacgattg gcccgtttaa tcgttgattc attcagtgct aaaaaatgct
   100741 ggtccggtgt ttcatccaaa taactgatcc gaacatccag caagggataa ctaacacttt
   100801 gttcccggtt cgttcgtaag ctgtctacta gttcttccag ctcgtaatcc cgccaatccg
   100861 tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg cacatcattc agacaatgac
   100921 tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc gactgagatg accaattctg
   100981 gctcatgatc atctcgttga atccctgctt gttctaaaag tgcgttcaaa cgttctgtca
   101041 cttctatagt ctttgcttct ttattatgaa cgatcgctac tttcatcccc tgcctcctct
   101101 ctttcgcttt ctttcttcaa aagcgttcga tagtagacat catctgattt tcctcttcca
   101161 tgggagaata accgctgtgc ttcttggatt tcttcacgga tactggacat ctcttcatcc
   101221 aactgatagg cagcttctgc cgcacgctga agtcgttcac tcatttcttc tggaaaaacg
   101281 ccttgatatt tataattcag cgaatgctca atcgttgccc agaaattcat cgcaagagtc
   101341 cgaatctgga tttccgccaa aattttcttc tcaccgctga tcagctgtac aggatactcg
   101401 atcaccagat gataagaacg gtagccactt gcttttttat ttgtgatgta atctcgttct
   101461 tggagaactt tcatgtcttt tcttttacgg atgatctcaa caacttgatg gatatcttct
   101521 acaaactgac acatgatcct taacccggca atatcagaca tttcttcttc taaacgtccc
   101581 attggaatat gccgtaattg tgattttgtt acaatactat cgactggctt gactctccca
   101641 gtgacaaatt caatcgggac atgtcgattt tgttcacgga actgtttgcg gatcccccgc
   101701 agtttcactt tcaattctga tacagtttgt tcatatggtg ctaaaaatgc ttcccaatct
   101761 cgttccatag ctgatacctc tacttcatta acattcattg tttattctac catatatgca
   101821 aagttttttc gtactcttga tttatgcatt gaccaaaaaa agaaaattag gcaaaataga
   101881 caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg aatataaatc tatgctgaca
   101941 aagcaagagt acgagatatt catcgctcac tatcaattaa cagataaaga tttccgtgta
   102001 cagactaata tttatttcga taccgttgat gaacagctga agaaacaaaa ttgtggccta
   102061 cggatccgtt tcgttggtca tcaagctgaa tatacattaa aaacacctgc tgaaaaaggg
   102121 cgtctcgaaa caacggatac ctttataaca gaagaagcag aagcatttat cttcgaaaaa
   102181 cgtctgcctc gttcaggcgc agtttttcaa aaactttctg atttgaatat cgatcctgct
   102241 tcattaatcc aaaccggaaa gcttacaaca aaacgggctg aattccctat tgaagaagga
   102301 cttttagcta ttgatgaaag ctggggagac aatctacacg attttgaact ggaactagag
   102361 gtaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg tttttcttta
   102421 ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa ttagaaacct
   102481 ttcattttca caaggcactt gagctcattt ttttactttt ttctgttaag ttataggatg
   102541 tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca tatgatcgaa
   102601 atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca acagttatta
   102661 caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt tcgctttctt
   102721 cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat ttctcagaac
   102781 aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt ggactgcaaa
   102841 gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca
   102901 gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt tgaagaaatc
   102961 ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa agaagtattc
   103021 ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt
   103081 tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg tacaagtagc
   103141 ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt ttttaatggt
   103201 gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt acacttgctt
   103261 tctaattaaa aaaatacgtc gactccctta caagagagcc gacgtatttt tttcgtttct
   103321 attcgagaaa atcaaaaata gaaagcctta taaacgatcg atcaattctt ctaattcatt
   103381 tagacgttgt tcaaacatcg acattgcatc ttcaatatat gcagcctgtg tcatatcaac
   103441 tcctgctttt ttcattactt ccacaggata atcgctgtta cctgctttta ggtaagccaa
   103501 gtagttctct agtgcctctg gttcctggtt tagtattttc ttcgccagtg ctgaagcagc
   103561 agaaaagcca gtcgagtatt ggaaaacata gtaattataa taaaaatgcg gtatccgtga
   103621 ccattcgaat ttaatttctg ggtcttcttc gactgctgga ccataatatt ttgcattcaa
   103681 tttaccataa ctatcactta ggtattcact agtcaatggc acaccttttt catcttccgt
   103741 atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc
   103801 atccaagtaa tggttgagta cataagcccg tacacgaggg tctttttctg tttccaataa
   103861 atactccgtt aggatatttt cattcgttgt cgaagcaatt tctgctaaaa agatggagta
   103921 gtcgccgtac acataaggct ggttcgaacg agtgaaataa ctatgaacac tatgtcccat
   103981 ttcgtggaca agcgtaaata gctgatccag cgtatcatgc caattcaata aaatatatgg
   104041 atttgtgtca tagcttcccg aagaataagc atcgcttcgt ttccctttat tttcgacaac
   104101 atcgatccaa cgttcagaga atgctttttc tacgatggcc atgtattctt cacccattgg
   104161 tttcagtgct tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc
   104221 ccctaagacc ggtgtataaa gatcatacat gtgcagtttt tccacttcta ataaacgttt
   104281 ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt
   104341 atcgtataca ctttcaggaa tatgattatt gctcaaagac gcttctctgg cagagctgta
   104401 attacggact ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa
   104461 tgtatttcta aattgttcgt aaacactgta caatccctta aatgctgctt cgcgcaccct
   104521 tcgatccgtg ctttctagca actgaccata cacgccatga gataattgga ctatttcacc
   104581 attttctcct tcaatcgttg gaaaaactag atctgcatta ttcaaaacag caaatgtatc
   104641 acttgaagca tcaaagattt cacctgctcc agcaagaaga gattcctgct cagcagataa
   104701 gacatgggct cgattatcta ctatttgctg gatataatgg cgatagactt ccaattttgg
   104761 ttcttctttg aaatattgcc aaatctggtc atctgacaat tgcaatattt ctggttcaaa
   104821 ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg
   104881 gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc gcatatacat aaatgacttc
   104941 agtttggcga taaaccctca atacgaattc aatcgcattt aaaaattgag aagcgccttg
   105001 atcaagtgtt cctttgtgtt tttcagattg ttttaactct tctgataatt ccaaatattt
   105061 ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta aattttcagg
   105121 caattcttct cgttttggca actgctttac ttccatgaaa tccactcctt ttcttccctt
   105181 agctctatct atcaaaatag atcatagtct aacatccaca tgtcacagca tctaactttt
   105241 ctactcattc atgtgtctgc ttccatcaat tttagcataa ctaaatcttg ttttacatag
   105301 aaaaccgatt cctagcctat ttttatactt actatttttt caaagaagac aagtgtatgc
   105361 attcgttaaa aaactgctga tagaccatct cttcatcaat cataggaaaa agccattccc
   105421 ttttatgttc ttttatttgg caaagccatg agttgaaatc aggaactttc tggttttcca
   105481 aggcttcttc gtacaacatt cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc
   105541 gacttttttg atagatccac gatgatagat gcaaaagatg tttatgacgc aagtagcatt
   105601 gttcttgtat ggatactgta ttcttttgct tgtgaataag ttggcgtgtc agccatttct
   105661 tcccatcttc actcactttt tctatttttt tcatttggtt cattttaatt tcgttactag
   105721 tgaatatttc tttaaggtcc ctactagaaa aagtccagca gctactgcaa aaggaaatat
   105781 gccctgatac agtctctgta atatgatgat acaaataaag cttttgtttc ttgagatcag
   105841 ctttccagca atgcactcct ctttttctat tatagtagca gcaacttttt tctatcaatg
   105901 agaattgcga ttgtccaaga aaagaattac caagtatcca ccatacttgg taactgttat
   105961 tcaaataatt ttgggttctt tcaataaatc tctggtgaga cagtctagaa cattggattt
   106021 ctattgctag attcgccaac aaaatatccg gcctttgccg caacctcggc aaataggctt
   106081 ccaaaaatac tggttctact gactgtccca gccaatccat gaagcattcc tttaactgta
   106141 agtgctcttt tgattctctt tcactaaaga ccgaacagtt atctgatttt tgatgggcaa
   106201 aatgagcaca ttttttcttt ccctttttca aaatcactct ttttcgacag ccacgacaat
   106261 aatatttctt tcctttttct gcatgtttag caaaacagca cttattatat tcattcattg
   106321 caagcagcat ttttctccct ctgaacttat ttacgtctaa gaatttattt acacgaaaaa
   106381 agagggcgtg aaaaagctat tctgcatcaa gtaataagaa cagctaaaca aaaatagttc
   106441 ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc aagcttttga acacatttat
   106501 acgaaaaaaa gactgcgaca caatttttgt cacagtctct tactataatg ctgatttata
   106561 caaaataaaa tttcgttcgc tcgatagcat tacgcgccat cagacattgt ccatattcat
   106621 tcaatgtttc tggtgtacga tcgctctttt cagcaaactc caaaattcga gcaatctgat
   106681 tctctacatc cgcttggttg agattttcca tccagaagtg aacgactagg taatagcgct
   106741 cttcgaataa atacaaatca gtccaagcag actctagttc gacatctgtt gcaagttgga
   106801 tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc
   106861 ttccagataa ttcttcctcc ggatcgctgc caataatctg ctgacgaaga aagtcactca
   106921 catcttctga atcaacagta gaatcttctg attgagaaat ttcttcatcc ggaagatttt
   106981 tactgataaa cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc
   107041 ttcctcggaa ctcctcttcg atatcaactt cttccaaaat actataaaag aaattttcga
   107101 tttctttgtg attgccaagt aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat
   107161 gaccaattag tacacggatc gtattttcat taatatgttc catttccatt ttgctacacc
   107221 tcactttgcc acaatgttta atatagattc attgtagcga aatcagctta aatgtaaaga
   107281 aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg gatttttcaa cctttcaagc
   107341 tatcttattt aactataaca tttttggcag acgaatacta tatagccgac ttttcagcta
   107401 attttccctc tctatttttc gtgcaatcat catcttctac taccttttta tacatctaat
   107461 caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta atagtctttt
   107521 ccaagtttcg tattatcctt ctataatcca gccatcaatt gtgcttgacg taattctaat
   107581 tgtcgtacat cgcgcggtag gaaccgacga atttcatctt cattaaaacc aacttgcaga
   107641 cgtttatcat caatcatgat tggacggcga agcaatccag gattgttttg cacaagttcc
   107701 aataattctt gtaacggcag atcatctaaa tccatgttca gtttttggaa tactttggaa
   107761 cgtgtcgaga tgatttcttc tgttccgtct tctgtcattt gtaaaatcgc ttttaattca
   107821 gaactgttca atggttctga aaaaatatta cgttcaacaa atggaatctg atgttcttgc
   107881 aaccacgcac gagcctttct gcaggaagta caacttgggg aagtatataa tgttaacaac
   107941 gtgcaccact ccttttcgtg ataagaatca cttttttgta acagaagatc ataataatct
   108001 cccatctgtt tctattatac ataatcaact tttaaatagc tacctttttt tcataatttt
   108061 tttaaaaaaa tgaaagttat ctaaggttaa tatacagtaa aaccagcatt ctcgcttttt
   108121 ttatcattta aaaattagag ttttcaaatc taattcaaat tttctaaaca tatgaaacag
   108181 cgtataaaac tgtactatta ttttatctca aaagctaaat gaaaccgatt tcttaagatt
   108241 ttttatgttt tttatttctt taatagtaat gttttctgtc attttaaata atactgttct
   108301 ttattataac gcttattttc tct
//
LOCUS       DACCQP010000002        94208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCQP010000002 DACCQP010000000
VERSION     DACCQP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.002"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            121..984
                     /locus_tag="IVO87_000106"
     CDS             121..984
                     /locus_tag="IVO87_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7448338.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1113..2735)
                     /locus_tag="IVO87_000107"
     CDS             complement(1113..2735)
                     /locus_tag="IVO87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7448339.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2984..3505
                     /locus_tag="IVO87_000108"
     CDS             2984..3505
                     /locus_tag="IVO87_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448340.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3691..5421)
                     /gene="spxB"
                     /locus_tag="IVO87_000109"
     CDS             complement(3691..5421)
                     /gene="spxB"
                     /locus_tag="IVO87_000109"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7448341.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5586..6404
                     /locus_tag="IVO87_000110"
     CDS             5586..6404
                     /locus_tag="IVO87_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7448342.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6507..6704)
                     /locus_tag="IVO87_000111"
     CDS             complement(6507..6704)
                     /locus_tag="IVO87_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448343.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6873..7451
                     /locus_tag="IVO87_000112"
     CDS             6873..7451
                     /locus_tag="IVO87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7448344.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8496..8999)
                     /locus_tag="IVO87_000113"
     CDS             complement(8496..8999)
                     /locus_tag="IVO87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7448345.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9358..10383
                     /locus_tag="IVO87_000114"
     CDS             9358..10383
                     /locus_tag="IVO87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7448346.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10706..11584
                     /locus_tag="IVO87_000115"
     CDS             10706..11584
                     /locus_tag="IVO87_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448347.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12060..12176
                     /locus_tag="IVO87_000116"
     CDS             12060..12176
                     /locus_tag="IVO87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7448348.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13104..13442
                     /locus_tag="IVO87_000117"
     CDS             13104..13442
                     /locus_tag="IVO87_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448349.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13536..13766
                     /locus_tag="IVO87_000118"
     CDS             13536..13766
                     /locus_tag="IVO87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448350.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14166..14537
                     /locus_tag="IVO87_000119"
     CDS             14166..14537
                     /locus_tag="IVO87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7448351.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14811..15503)
                     /locus_tag="IVO87_000120"
     CDS             complement(14811..15503)
                     /locus_tag="IVO87_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7448352.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15506..16291)
                     /locus_tag="IVO87_000121"
     CDS             complement(15506..16291)
                     /locus_tag="IVO87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7448353.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16528..17928
                     /gene="lacG"
                     /locus_tag="IVO87_000122"
     CDS             16528..17928
                     /gene="lacG"
                     /locus_tag="IVO87_000122"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7448354.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17953..18270
                     /locus_tag="IVO87_000123"
     CDS             17953..18270
                     /locus_tag="IVO87_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7448355.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18283..19956
                     /locus_tag="IVO87_000124"
     CDS             18283..19956
                     /locus_tag="IVO87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7448356.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19970..21601
                     /locus_tag="IVO87_000125"
     CDS             19970..21601
                     /locus_tag="IVO87_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7448357.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22092..23657)
                     /gene="guaA"
                     /locus_tag="IVO87_000126"
     CDS             complement(22092..23657)
                     /gene="guaA"
                     /locus_tag="IVO87_000126"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7448358.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23716..24639)
                     /locus_tag="IVO87_000127"
     CDS             complement(23716..24639)
                     /locus_tag="IVO87_000127"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7448359.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24835..25440
                     /locus_tag="IVO87_000128"
     CDS             24835..25440
                     /locus_tag="IVO87_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7448360.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25748..26764
                     /locus_tag="IVO87_000129"
     CDS             25748..26764
                     /locus_tag="IVO87_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7448361.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26905..28206
                     /locus_tag="IVO87_000130"
     CDS             26905..28206
                     /locus_tag="IVO87_000130"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7448362.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28252..28914
                     /gene="deoC"
                     /locus_tag="IVO87_000131"
     CDS             28252..28914
                     /gene="deoC"
                     /locus_tag="IVO87_000131"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7448363.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28935..29333
                     /locus_tag="IVO87_000132"
     CDS             28935..29333
                     /locus_tag="IVO87_000132"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7448364.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29399..30481
                     /locus_tag="IVO87_000133"
     CDS             29399..30481
                     /locus_tag="IVO87_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7448365.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30693..32255
                     /locus_tag="IVO87_000134"
     CDS             30693..32255
                     /locus_tag="IVO87_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448366.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32248..33381
                     /locus_tag="IVO87_000135"
     CDS             32248..33381
                     /locus_tag="IVO87_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448367.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33381..34337
                     /locus_tag="IVO87_000136"
     CDS             33381..34337
                     /locus_tag="IVO87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448368.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34436..35602
                     /gene="deoB"
                     /locus_tag="IVO87_000137"
     CDS             34436..35602
                     /gene="deoB"
                     /locus_tag="IVO87_000137"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7448369.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35626..36444
                     /locus_tag="IVO87_000138"
     CDS             35626..36444
                     /locus_tag="IVO87_000138"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7448370.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36461..37168
                     /gene="deoD"
                     /locus_tag="IVO87_000139"
     CDS             36461..37168
                     /gene="deoD"
                     /locus_tag="IVO87_000139"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7448371.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37466..38152)
                     /gene="gpmA"
                     /locus_tag="IVO87_000140"
     CDS             complement(37466..38152)
                     /gene="gpmA"
                     /locus_tag="IVO87_000140"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7448372.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38324..38905)
                     /locus_tag="IVO87_000141"
     CDS             complement(38324..38905)
                     /locus_tag="IVO87_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7448373.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39038..39721)
                     /gene="rpiA"
                     /locus_tag="IVO87_000142"
     CDS             complement(39038..39721)
                     /gene="rpiA"
                     /locus_tag="IVO87_000142"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7448374.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40040..40453
                     /gene="rpsL"
                     /locus_tag="IVO87_000143"
     CDS             40040..40453
                     /gene="rpsL"
                     /locus_tag="IVO87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7448375.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVRD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40537..41007
                     /gene="rpsG"
                     /locus_tag="IVO87_000144"
     CDS             40537..41007
                     /gene="rpsG"
                     /locus_tag="IVO87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7448376.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41101..43188
                     /gene="fusA"
                     /locus_tag="IVO87_000145"
     CDS             41101..43188
                     /gene="fusA"
                     /locus_tag="IVO87_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7448377.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43360..44547
                     /gene="tuf"
                     /locus_tag="IVO87_000146"
     CDS             43360..44547
                     /gene="tuf"
                     /locus_tag="IVO87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7448378.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44954..45262
                     /gene="rpsJ"
                     /locus_tag="IVO87_000147"
     CDS             44954..45262
                     /gene="rpsJ"
                     /locus_tag="IVO87_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7448379.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45293..45922
                     /gene="rplC"
                     /locus_tag="IVO87_000148"
     CDS             45293..45922
                     /gene="rplC"
                     /locus_tag="IVO87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7448380.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45950..46573
                     /gene="rplD"
                     /locus_tag="IVO87_000149"
     CDS             45950..46573
                     /gene="rplD"
                     /locus_tag="IVO87_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7448381.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46573..46863
                     /gene="rplW"
                     /locus_tag="IVO87_000150"
     CDS             46573..46863
                     /gene="rplW"
                     /locus_tag="IVO87_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7448382.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46902..47735
                     /gene="rplB"
                     /locus_tag="IVO87_000151"
     CDS             46902..47735
                     /gene="rplB"
                     /locus_tag="IVO87_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7448383.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47778..48056
                     /gene="rpsS"
                     /locus_tag="IVO87_000152"
     CDS             47778..48056
                     /gene="rpsS"
                     /locus_tag="IVO87_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7448384.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48079..48426
                     /gene="rplV"
                     /locus_tag="IVO87_000153"
     CDS             48079..48426
                     /gene="rplV"
                     /locus_tag="IVO87_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7448385.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48440..49096
                     /gene="rpsC"
                     /locus_tag="IVO87_000154"
     CDS             48440..49096
                     /gene="rpsC"
                     /locus_tag="IVO87_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7448386.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49099..49533
                     /gene="rplP"
                     /locus_tag="IVO87_000155"
     CDS             49099..49533
                     /gene="rplP"
                     /locus_tag="IVO87_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7448387.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49523..49711
                     /gene="rpmC"
                     /locus_tag="IVO87_000156"
     CDS             49523..49711
                     /gene="rpmC"
                     /locus_tag="IVO87_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7448388.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49736..50002
                     /gene="rpsQ"
                     /locus_tag="IVO87_000157"
     CDS             49736..50002
                     /gene="rpsQ"
                     /locus_tag="IVO87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7448389.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50060..50428
                     /gene="rplN"
                     /locus_tag="IVO87_000158"
     CDS             50060..50428
                     /gene="rplN"
                     /locus_tag="IVO87_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7448390.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50465..50773
                     /gene="rplX"
                     /locus_tag="IVO87_000159"
     CDS             50465..50773
                     /gene="rplX"
                     /locus_tag="IVO87_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7448391.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50801..51340
                     /gene="rplE"
                     /locus_tag="IVO87_000160"
     CDS             50801..51340
                     /gene="rplE"
                     /locus_tag="IVO87_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7448392.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51359..51544
                     /locus_tag="IVO87_000161"
     CDS             51359..51544
                     /locus_tag="IVO87_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7448393.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51580..51978
                     /gene="rpsH"
                     /locus_tag="IVO87_000162"
     CDS             51580..51978
                     /gene="rpsH"
                     /locus_tag="IVO87_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7448394.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52010..52546
                     /gene="rplF"
                     /locus_tag="IVO87_000163"
     CDS             52010..52546
                     /gene="rplF"
                     /locus_tag="IVO87_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7448395.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52707..53063
                     /gene="rplR"
                     /locus_tag="IVO87_000164"
     CDS             52707..53063
                     /gene="rplR"
                     /locus_tag="IVO87_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7448396.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53084..53584
                     /gene="rpsE"
                     /locus_tag="IVO87_000165"
     CDS             53084..53584
                     /gene="rpsE"
                     /locus_tag="IVO87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7448397.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53599..53778
                     /gene="rpmD"
                     /locus_tag="IVO87_000166"
     CDS             53599..53778
                     /gene="rpmD"
                     /locus_tag="IVO87_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7448398.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53822..54262
                     /gene="rplO"
                     /locus_tag="IVO87_000167"
     CDS             53822..54262
                     /gene="rplO"
                     /locus_tag="IVO87_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7448399.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54262..55557
                     /gene="secY"
                     /locus_tag="IVO87_000168"
     CDS             54262..55557
                     /gene="secY"
                     /locus_tag="IVO87_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7448400.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55619..56266
                     /locus_tag="IVO87_000169"
     CDS             55619..56266
                     /locus_tag="IVO87_000169"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7448401.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56457..56675
                     /gene="infA"
                     /locus_tag="IVO87_000170"
     CDS             56457..56675
                     /gene="infA"
                     /locus_tag="IVO87_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7448402.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56708..56824
                     /gene="rpmJ"
                     /locus_tag="IVO87_000171"
     CDS             56708..56824
                     /gene="rpmJ"
                     /locus_tag="IVO87_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7448403.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56842..57207
                     /gene="rpsM"
                     /locus_tag="IVO87_000172"
     CDS             56842..57207
                     /gene="rpsM"
                     /locus_tag="IVO87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7448404.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57235..57624
                     /gene="rpsK"
                     /locus_tag="IVO87_000173"
     CDS             57235..57624
                     /gene="rpsK"
                     /locus_tag="IVO87_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7448405.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57704..58642
                     /locus_tag="IVO87_000174"
     CDS             57704..58642
                     /locus_tag="IVO87_000174"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7448406.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58670..59050
                     /gene="rplQ"
                     /locus_tag="IVO87_000175"
     CDS             58670..59050
                     /gene="rplQ"
                     /locus_tag="IVO87_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7448407.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59322..60161
                     /locus_tag="IVO87_000176"
     CDS             59322..60161
                     /locus_tag="IVO87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7448408.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60137..61006
                     /locus_tag="IVO87_000177"
     CDS             60137..61006
                     /locus_tag="IVO87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7448409.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            60996..61793
                     /locus_tag="IVO87_000178"
     CDS             60996..61793
                     /locus_tag="IVO87_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7448410.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            61996..62742
                     /gene="truA"
                     /locus_tag="IVO87_000179"
     CDS             61996..62742
                     /gene="truA"
                     /locus_tag="IVO87_000179"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7448411.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(63197..65866)
                     /locus_tag="IVO87_000180"
     CDS             complement(63197..65866)
                     /locus_tag="IVO87_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7448412.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(66104..66292)
                     /locus_tag="IVO87_000181"
     CDS             complement(66104..66292)
                     /locus_tag="IVO87_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7448413.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            66562..66924
                     /locus_tag="IVO87_000182"
     CDS             66562..66924
                     /locus_tag="IVO87_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7448414.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            66976..68658
                     /locus_tag="IVO87_000183"
     CDS             66976..68658
                     /locus_tag="IVO87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7448415.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            68775..70811
                     /gene="recG"
                     /locus_tag="IVO87_000184"
     CDS             68775..70811
                     /gene="recG"
                     /locus_tag="IVO87_000184"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7448416.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            70860..71861
                     /gene="plsX"
                     /locus_tag="IVO87_000185"
     CDS             70860..71861
                     /gene="plsX"
                     /locus_tag="IVO87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7448417.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            71983..72225
                     /gene="acpP"
                     /locus_tag="IVO87_000186"
     CDS             71983..72225
                     /gene="acpP"
                     /locus_tag="IVO87_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7448418.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            72493..73497
                     /locus_tag="IVO87_000187"
     CDS             72493..73497
                     /locus_tag="IVO87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448419.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            73498..74442
                     /locus_tag="IVO87_000188"
     CDS             73498..74442
                     /locus_tag="IVO87_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448420.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            74442..75404
                     /locus_tag="IVO87_000189"
     CDS             74442..75404
                     /locus_tag="IVO87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448421.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            75431..76345
                     /locus_tag="IVO87_000190"
     CDS             75431..76345
                     /locus_tag="IVO87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448422.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            76371..78152
                     /locus_tag="IVO87_000191"
     CDS             76371..78152
                     /locus_tag="IVO87_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7448423.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            78500..79186
                     /locus_tag="IVO87_000192"
     CDS             78500..79186
                     /locus_tag="IVO87_000192"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7448424.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            79206..82787
                     /gene="smc"
                     /locus_tag="IVO87_000193"
     CDS             79206..82787
                     /gene="smc"
                     /locus_tag="IVO87_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7448425.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            82796..83605
                     /locus_tag="IVO87_000194"
     CDS             82796..83605
                     /locus_tag="IVO87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7448426.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            83616..84614
                     /gene="ftsY"
                     /locus_tag="IVO87_000195"
     CDS             83616..84614
                     /gene="ftsY"
                     /locus_tag="IVO87_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7448427.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            84801..87068
                     /gene="gshAB"
                     /locus_tag="IVO87_000196"
     CDS             84801..87068
                     /gene="gshAB"
                     /locus_tag="IVO87_000196"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7448428.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(87157..87609)
                     /locus_tag="IVO87_000197"
     CDS             complement(87157..87609)
                     /locus_tag="IVO87_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7448429.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            87759..88709
                     /locus_tag="IVO87_000198"
     CDS             87759..88709
                     /locus_tag="IVO87_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7448430.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            88702..89661
                     /locus_tag="IVO87_000199"
     CDS             88702..89661
                     /locus_tag="IVO87_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7448431.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            89658..90413
                     /locus_tag="IVO87_000200"
     CDS             89658..90413
                     /locus_tag="IVO87_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7448432.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            90436..91389
                     /locus_tag="IVO87_000201"
     CDS             90436..91389
                     /locus_tag="IVO87_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7448433.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(92033..92329)
                     /locus_tag="IVO87_000202"
     CDS             complement(92033..92329)
                     /locus_tag="IVO87_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7448434.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            92684..93040
                     /locus_tag="IVO87_000203"
     CDS             92684..93040
                     /locus_tag="IVO87_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7448435.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            93050..93949
                     /locus_tag="IVO87_000204"
     CDS             93050..93949
                     /locus_tag="IVO87_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7448436.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        30294 a        16803 c        20084 g        27027 t
ORIGIN      
        1 gtcacaacct cttattttga ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc
       61 gcaagagatt tgctataata gacgataagg gtaaaatgta acgagaaggg gttgaaaaat
      121 gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat accatgtatt agcaaaagaa
      181 aaagttgatt ttttatcaat gaatctccaa ttgaatgatc aagagttagt agatgacctc
      241 ggaaagacat tcaattatga ccgctacatg aatcagctga aaaatggtgc gatgcctacg
      301 acatcacaaa tcaatgttgg ccgatattta gaatttttcc gttcttatgc ggaaaaacag
      361 attcctgttt tatatgtggc tttttccagt ggattaagcg gctcttacag tagtgcgctt
      421 caagcggttg aattgctgaa ggaagaatat gaagaccctg agatctatgt ttttgataca
      481 aaagctgcaa gtattgggga agggctttta gtattggaag ctgtaagatt gaaaaaagaa
      541 ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta attatttgaa agtccattca
      601 tgggtaacag tagacgatct gaaatatcta gaacgaggcg gtaggatttc caaagcaagt
      661 gctactcttg gcagtctatt gagtgtaaag cctattatta cggtggataa agaaggaagg
      721 ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga tccagacaat cgtcaatgag
      781 acggtaaaaa caatcgttgc accacttgat caaatcgttt atgtggctta cgctggggat
      841 ctagagagcg cggagaaggc aaaacggctt ttggaagagc agataaagat gaaagatgtg
      901 aagctttatc ccttaggacc aacgatcgcc agtcacacag gctatggctg tatcgcgatt
      961 ttttcgatgg gaatcagtcg ataaaagcag taactcattc tgtaaaaaag cccagaaatt
     1021 gattgtaaca aaaacagcga ctcttaaaag atagatgagc atcctatctc ttagaggtcg
     1081 ctgttttttt attttttaag ctaaataggc cattatgccc ataactcgtt caattgtttt
     1141 tgaatatctg cattttccaa aaattcatca tatgtcgtat cagctcgatc gatcacacct
     1201 ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga taaattgatg gtcatgtgac
     1261 gcaaataaga ttgagccagt aaatgccatc aagccgtcat tcaatgctgt gattgattct
     1321 aagtctaaat ggttcgttgg atcatctaat acaagtacgt tagctttcga aagcatcatt
     1381 tttgaaagca tgcaacggac tttctctcca ccagataaaa cattcactgg tttcaatact
     1441 tcttcaccag aaaacaacat tcttcctaag aagcttctta agaaggtgtt atcatcttct
     1501 tctttacttg catattgacg caaccagtct aaaatcgtta gatcagtatc gaattctttc
     1561 gttgtatcct ttggtaaata agaattactt gttgtcacac cccaacgtac tgttccagta
     1621 tctggcgtaa tttcacccat gataactttg aatagcgttg ttgttgttat atcattttct
     1681 gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag atatatcatc taaaattttc
     1741 tttccatcaa tcgttacact tacattttct acctgcaata agtcgtttcc aatctcacgt
     1801 tcaggtttga atccaacgaa tggataacgt ctagaagaag gttggatatc gtctaaagta
     1861 attttttcaa gcattttttt acgtgaagtt gcttgttttg attttgatgc attcgcacta
     1921 aagcgggcaa taaattcttg gagttctttg atttgttctt cttttttggc atttgcgttt
     1981 gcttgaagtt tagccgctag ttgactagat tccaaccaaa aatcataatt tcctacataa
     2041 agtttgattt taccaaaatc tagatccgcc atatgtgtac atactttgtt caaaaagtga
     2101 cggtcatggg atacaacgat cacagtattt tcaaaattga tcaaaaattc ttccagccaa
     2161 ttgatcgatt ggatatccag accgtttgtt ggttcgtcta gcaaaagaac atcaggttta
     2221 ccaaatagtg tttgtgctaa taacactttt accttttgtc caccagttaa ttcactcatt
     2281 tgctgatggt gcaattcttc agggatgttc agaccttgca gtaaaacagc tgcttctggt
     2341 tcagcttccc atccattaag ctcagcaaat tctccttcta attcagctgc tcgaatacca
     2401 tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt tttctttcag cacttcatac
     2461 agacgcttgt gtcccatgat gactgtttca agtacagtat agtcttcata gtcgtaatgg
     2521 ttctgtttca atacggccat ccgttcatct ggtcccatag taacggaacc tgtggaaggc
     2581 tgcagatccc ctgataacac tttaaggaac gttgatttac ctgcaccatt tgcaccaatc
     2641 aagccatagc agtttccggg ggtaaattta atattcacat catcaaatag tttgcggtct
     2701 gaaaagtgca aactcacatc atttacagta atcaattttt ttcctcactt ttatcattat
     2761 tagttttgac aagtcttata gaattatata ggggaaaggt aaaagattca aggcaatcga
     2821 acattctttg tgtttcacgt gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa
     2881 atagctacta taggatttat tagtttgcaa cagcataatt tttgacgtta tttataagag
     2941 ggagtactat aaaactgtaa aagaaatgaa agaaggattg aaaatggatg aaaaaagaac
     3001 catcgactgg ggctcattga ttttgggaat tttatttgta cttgtctcat tgttatcatt
     3061 tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc tttgcgattt ttgcctttat
     3121 taaagggata tttgaactgt ttgtacggaa cagattaaaa gagctaacag gatataaagg
     3181 aaagatgccc ttaataatcg ggatcatcga tattctagtt ggcgtctttt tcttattcaa
     3241 tattggtgca ggagtcgcag cattgccttt tgtttttgca gtatggttta ttgctgattc
     3301 agttttagcg ttatttactg ctgacttggc aagaggagta agtgaaggat attattggtt
     3361 tacgatcatc gtcaatattt taggaatttt attaggaatt ttcttattgt ttaatccaat
     3421 ttcttctgcg ttgacattaa gctttttagt tggattctat tttatgttat ttggtatcac
     3481 acatatcgtc tatgcattta gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat
     3541 tacagtttta ctgttgtaat tggaagccgg cttttttatc atggacaaat atgcacgagg
     3601 tgatttttag gtgtcgagac aaatttatgc aaaaatgtga gaagctatat acacaaattt
     3661 tatcttaata ctcgaagcat agcgcagttg tcacaacctc ttttcatata attcattcaa
     3721 tactggcaaa tcatggactt catatttttc tttgaatgct tggatttctt cttcagaata
     3781 ttttcttgga tctaaatgca gatcttctac tggcaatggg cgcttgttat tgatcttcac
     3841 atcgattact acaggacgat ctgattttgc agctgcatca aaggcaaatc gaagttgcga
     3901 gtattctgtg actgtaaagc tatttgcccc gagcgcttct ccaactttac cgaaatctgc
     3961 atcttctaaa aatacaccaa atttcttttg ttccgtatct tcttgttctg cttcgatgaa
     4021 accaaaagaa tcattcgaaa agacaacatt aataattgga agctgatatt tgacttgtgt
     4081 aatgatatct tgcatgacca tagcaaaccc gccatcacca cttaaggtaa atacttgttt
     4141 ttctgggtag cttagttgtg cggcaattcc tcctggcaca ccattaccca tagttgcgaa
     4201 ccatccagat gtcgtatgtt tttgtttacc gttcatattc aataaacgaa tagaatggat
     4261 agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac gattgatttc
     4321 tttatagaca gcctctggac gaatcggttc ttcctctcga tcttcaaaag atttcaacca
     4381 atttacccat ttttctttat tttcttgatt tgctaggtac cagctatcag cagggcgagc
     4441 atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga
     4501 tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg atttggataa attcagcagt
     4561 tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg acaaataaga tcagatcggc
     4621 ttctgccaat gcttcatttc cgggtttagt agcgacacgt ccagcaaaac caagaaagtt
     4681 ttcataatca tcaggaataa ttccttttgc tagaacggaa gcgacaacag gaactgaaaa
     4741 atgttctgag aattctttga tttgcggaaa tccgtttcgc gttccttgac caacataaag
     4801 aactggtttt tttgcttttt caagataagg caaagcagct agcaaatctt tttcttctgg
     4861 taatatcaca cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc
     4921 aaaaccaaga tcaacaggga tagtcacgat agccacgcct ttatgctcat aggcggcttt
     4981 aatggcttca tctactacat gcggaagact ttgtggtgtc atgactgtac gattgtatac
     5041 gctaacatct gcgaaaatag gattttcatt tagttcctgg aaagagttat agttcatgga
     5101 tgtagaagca acttgtccta agaacgctac tagaggcaca tggtccatct gtgcatcata
     5161 caaaccattg atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag cgccgacttt
     5221 ccccgttaat tttgcatctg cagcggctgc gagtgcgccg acttcttcat gacgaacttg
     5281 aatgtagttg atttcggttt tttcttgata tagcgcatcc ataattgaat tgaaagatcc
     5341 tccgggaatg ccgtaaatat ggtcgatacc ccagctttca agaactttta ccattgcgat
     5401 tcctgcattg atttttgaca tggtgtttca ctcctttagc aatgatgata cataaatttt
     5461 ttgtatctag agtatagaac tttttatcga aaatcccaac taattcgtct tttcacaaaa
     5521 atgtagtcat gtttttggga atatgctaga gtagtacata gatagattca agagagggga
     5581 atggcatggt tgaatggcta ttgagacttg aaccttggca gcaagcactt gctgggacag
     5641 cttttactta ttttatgaca gctcttggtg ccgggttggt ttttttcttt aaagaaatca
     5701 aaaaagaagt gctaaacttg atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt
     5761 tttggtcttt gttagatccg gctattacaa aagcagaaga aaatggagat attgcttggc
     5821 tagttgtaag tattgggttt ggtttaggcg gtgtgttttt atatatggca gataaaacac
     5881 tgccccatat gcattttgga ccacaacatg aagcagaagg attgcctacc catttaaaac
     5941 ggacgatctt gcttgttttt tctattacat tgcataacat tcctgaaggt ttagccgtgg
     6001 gggtagcatt cggtgcagca gcaactgcgg ataatccgac agccgctata ttagccgctg
     6061 tttcggtggc gctaggtatt ggtatccaaa acttcccaga aggagcagct gtatcaatcc
     6121 cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa
     6181 tcgttgaacc aattgctgga gtgattggag cgctactagt aacgaaagtg gagctgctgc
     6241 ttccttatgc acttgctttt gccgcaggag caatgattta tgtcgtggtg gaagaattga
     6301 tccctgaagc acagcagaca ttgtccagca aacgtcattt tgcagttttt ggcgtcatga
     6361 gtggatttat cattatgatg attttggatg tggcattagg ataaataaaa aaaatatata
     6421 tgatatatca aatagggccg cgaacttata tcgcgaccct atttttttag taagttatat
     6481 cataatgaag tgaatggtat catacattaa taatagtttt ttctatgtgt atttcctttg
     6541 atccagcgaa caagccagat gataagtgcg attgggatta gaactttgac cgcaaaccat
     6601 aaaagcccgc ttaggacact gacaaaaaga ctgatgacga agctagctgc aattagcact
     6661 aaaagaacca tgaagatatt taagaacata tttttttgac gcatacaaaa ccctcctctt
     6721 gttcattctt tactatagaa aaagtataac agggattgtt taaaaacacc tccaactgaa
     6781 gactgatttt tacttcatct acttaaatga tcaaacaatt gctttttctc tggtttatca
     6841 tctaaaatag agcggaaaag gaggagaata tcatgcctaa aaatcgtctt gaagcattta
     6901 cagatgctgt aatcgcgatt atcatgacta ttttagtttt agaacttcat gaacctaacg
     6961 gagacacgtg ggaggcgctc gctgcattag gttaccgatt ttttatctat attgtcagtt
     7021 ttatcatttt agcgatttat tggaacaatc atcatcattt atttcaagtt gtacataaga
     7081 taaatggacg ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt
     7141 ttgcaacttc atggatcagt gagtacatca atagcctggc tccagaaatg acttatggtt
     7201 ttgttacctt gaccgctaat attgcgtatt atatcttggc aaaagaactt gtcaaagcaa
     7261 ataaaggcca cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat
     7321 cgattggttt gaatgtgtta ggaattgtct tagggtattt agtagcgcca gtagccgtgc
     7381 tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc aagtcgtacc atcgaatcac
     7441 aatatcggta aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa aaataaataa
     7501 acgatcaaat atccgagtga tactagtatt caaagaggga tgcagatgga tcaaaaaaga
     7561 attcatgtag gactgattac tagttttgtc acgatgctta tttatttgga tcaaggtcat
     7621 ttgacttgga cagtagttgg tgtcgtagga tttttgacat tattcacagc aataaaaaaa
     7681 taaacatctt tatgtaatct gatgttctat ctatttaaga aaagcatgtt tacagcagta
     7741 tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta atttttagaa aagtgtatcg
     7801 aaaaatagat agttagtttt tccacagtgg ataacttcgt aagagataaa aataaaaaat
     7861 tagtcaaaga agggcgtata gatttctttt gataggagat atacaacaga aatgaataaa
     7921 tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt tggaataaaa aaataacata
     7981 tctcatttta cacttttgtt attttatact taaaaaataa cgacacagaa aataccgtgt
     8041 ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa
     8101 agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa
     8161 aaaccataga aaatataaag taattactta taaaacaagc tttttataga aaaataatat
     8221 acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa
     8281 gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa
     8341 tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga
     8401 tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg
     8461 aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc
     8521 aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga
     8581 cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc
     8641 agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg
     8701 aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa
     8761 gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta
     8821 taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga
     8881 ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa
     8941 atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata
     9001 gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta
     9061 tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa
     9121 tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc
     9181 tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt
     9241 gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg
     9301 gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg
     9361 ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat catgaatctg
     9421 atgctactta tctttattgt attatggtta ggttacataa atcatgattt tttgatttca
     9481 agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat
     9541 ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc
     9601 ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc
     9661 agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt
     9721 tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat
     9781 ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca
     9841 cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta
     9901 gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac
     9961 ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca
    10021 gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg
    10081 ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg
    10141 atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat
    10201 atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt
    10261 tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt
    10321 gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa
    10381 tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa
    10441 ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa
    10501 aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac
    10561 tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct
    10621 tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt
    10681 gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat
    10741 gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt
    10801 tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag
    10861 tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta
    10921 tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa
    10981 aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt
    11041 tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt
    11101 ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat
    11161 gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct
    11221 tcatattcct tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc
    11281 cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac
    11341 ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa tctgtctttt
    11401 tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat
    11461 ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca
    11521 agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc
    11581 ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt
    11641 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
    11701 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
    11761 aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg
    11821 taaactagct ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc
    11881 taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga
    11941 ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa
    12001 ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata
    12061 tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac
    12121 gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag
    12181 aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc aataatcggt
    12241 ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca tatgataaat
    12301 tggtagaatc tttcttttca aattgatacc attccctaat tccagttata tagttaacta
    12361 cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt tcaattccct
    12421 ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac ctttgttcgt
    12481 ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg ttttgcgaaa
    12541 tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac cattatcgtt
    12601 taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc atttcttttc
    12661 ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa ctttaaaaag
    12721 taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag aataacatca
    12781 aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg gaattttaga
    12841 tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa gtatctttaa
    12901 taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt gtagtcagtc
    12961 gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga ttggaagata
    13021 aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt attttccctc
    13081 tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa catgataacc
    13141 tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg gagactccat
    13201 tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat atacagtacg
    13261 aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta gagctaacac
    13321 cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt gacgagactg
    13381 agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata ttgtggatgt
    13441 agacactagt gaaatacttt atgatataga aattgatgaa aatttactag atgtagcaca
    13501 agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag ggaaaagaaa
    13561 atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg aaagaattca
    13621 agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa ggatcgatag
    13681 aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta gaagagtttt
    13741 tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt tgacacatat
    13801 tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat gcagtataaa
    13861 gagtatccag agtattttat tttaactttt gtatatttag taagtagcta cacacttcta
    13921 tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag cttattgatt
    13981 ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac actcagagaa
    14041 cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct ggttgggaaa
    14101 acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag tatgccgata
    14161 ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa tcccagtcat
    14221 taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct tcagggatta
    14281 gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa attgtacgca
    14341 tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt aatgatactt
    14401 acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa acgtttattt
    14461 cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt ggagaggaac
    14521 tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa aaaagtactc
    14581 ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt aatgttaatt
    14641 atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag atacactgct
    14701 atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca gttttttata
    14761 aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt ttaagaatta
    14821 acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat ggttccatct
    14881 ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc aattgcaata
    14941 attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc ttcgataaca
    15001 agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat atccggttgc
    15061 ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa ccagcgggct
    15121 aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt ggcaatcgtt
    15181 aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt tgtcaactga
    15241 agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc ttctgacaat
    15301 acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat ctccgtattc
    15361 gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt tgtatgatca
    15421 tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat tgttccgttg
    15481 aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg gttgaatcag
    15541 caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag atctggcgat
    15601 ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca tctcgattga
    15661 attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg atctgctgtg
    15721 cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca tttgaactga
    15781 taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt ccgacaaatg
    15841 cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca tctgcttgag
    15901 caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg ttcagtaaat
    15961 gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct ccctcgtgaa
    16021 tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga gtggcttttt
    16081 cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct ccatggacac
    16141 gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc atatcagaaa
    16201 catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg accatttcta
    16261 cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta aggataatct
    16321 atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt agggtggttt
    16381 ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta tttgtgtttg
    16441 tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa gagcgaaaac
    16501 tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt tatttttggc
    16561 ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa aggcgctgtt
    16621 gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc aagtgatttt
    16681 tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa tggtctaaga
    16741 ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa tccagaagga
    16801 atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac gccgtttgtg
    16861 acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt tttgaatcga
    16921 gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt caaagaagtc
    16981 aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata tttgttaggt
    17041 gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct tcataatatg
    17101 atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc gggtgaaatt
    17161 ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga agataagcat
    17221 gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac ctatttaggc
    17281 tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa tcaggccagc
    17341 tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg taatgactac
    17401 ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga aaatgcaatc
    17461 catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg aatcggagaa
    17521 cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta tccagaaggt
    17581 ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa aatctatatc
    17641 actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat ggatcagccg
    17701 agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac agccggggta
    17761 aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac aaatgggtat
    17821 aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta cccaaaagaa
    17881 tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata agtgataatg
    17941 gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat cgttgcatat
    18001 tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca aggaaacttt
    18061 gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc acataattcg
    18121 caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg attcattttt
    18181 atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat tcaagacttt
    18241 attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg aatcattaaa
    18301 caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta tttaggagcc
    18361 gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat tttcattttg
    18421 attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc cacatggcta
    18481 tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc aaccactgct
    18541 cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat caacaccacc
    18601 tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc gattgaaggt
    18661 ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt cgctgcattt
    18721 ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa aatgcctaaa
    18781 gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc tttttcagta
    18841 tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc atttgcagaa
    18901 gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt aggaatcgct
    18961 attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc gtcgatcgtg
    19021 gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca attgttcaat
    19081 tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt aggaactatg
    19141 ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc taagtctaag
    19201 cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt caacgaacca
    19261 ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt tttgttaaca
    19321 cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat gaacagcttt
    19381 atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg aacgggcatg
    19441 ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct gatctatcta
    19501 ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa agcagaggtg
    19561 atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt ggcagcgagt
    19621 caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc tggcgcaggg
    19681 acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa agcaccgatc
    19741 agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt tgatatggtg
    19801 gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac cgatcgctta
    19861 ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag agatcctgaa
    19921 aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa tgaaaatggg
    19981 aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg gggctgtttc
    20041 aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt cgatcctgtc
    20101 agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag atgccttaga
    20161 gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg atccttataa
    20221 tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga ttgaccttgc
    20281 aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca gtgatttttg
    20341 ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct ttgaagaaat
    20401 gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc agctggacgt
    20461 ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct ggccttatgg
    20521 caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt tgaagtctgg
    20581 tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc acttagctga
    20641 tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc gaggtgtgtc
    20701 atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga atgaacttca
    20761 aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag ttgaaacagc
    20821 ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg aagccgaagc
    20881 agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc tggatcttgc
    20941 cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt gggaacctct
    21001 ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc tcagtgttca
    21061 atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg gaaacgcctt
    21121 gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta aaaatgcaag
    21181 tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt ttcaacaagg
    21241 aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact acaaactaac
    21301 tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg gcataagcaa
    21361 aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata ctaatcagtt
    21421 atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa caagtgaatg
    21481 gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa ttggggttta
    21541 tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg tgaaacaata
    21601 gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg ttagcgtaga
    21661 aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg atttccagtc
    21721 atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta tgctaaagag
    21781 agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca gataacataa
    21841 agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg agcacttaga
    21901 tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt ttatgataag
    21961 aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct tatttttaat
    22021 gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc cctccatatt
    22081 atgttggctc tttattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg
    22141 atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc
    22201 catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg
    22261 actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga
    22321 acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag
    22381 atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga
    22441 ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac
    22501 tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca
    22561 aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt
    22621 tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct
    22681 tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg
    22741 attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt
    22801 gcattgactt tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc
    22861 gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg gtcaccgatc
    22921 gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag
    22981 acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc
    23041 attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt
    23101 ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg
    23161 tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga
    23221 actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta
    23281 aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta
    23341 gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct
    23401 aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat
    23461 acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct
    23521 gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg
    23581 atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata
    23641 tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca
    23701 aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt
    23761 tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt
    23821 caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat
    23881 agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa
    23941 acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc
    24001 aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc
    24061 aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac
    24121 atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa
    24181 atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg
    24241 ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct
    24301 tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac
    24361 ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa
    24421 taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc
    24481 aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat
    24541 attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc
    24601 ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc
    24661 atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc
    24721 gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac
    24781 ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca
    24841 aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag
    24901 ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg
    24961 gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga
    25021 aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca
    25081 cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac
    25141 gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt
    25201 catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt
    25261 gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt
    25321 gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt
    25381 aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa
    25441 ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt
    25501 atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag
    25561 aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac agaacttgta
    25621 tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt
    25681 ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc
    25741 caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac
    25801 tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt
    25861 gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa
    25921 tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa
    25981 actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa
    26041 ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt
    26101 ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg
    26161 tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg
    26221 acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa
    26281 tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg
    26341 tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt
    26401 tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca
    26461 taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc
    26521 gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt
    26581 aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat
    26641 tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc
    26701 gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg
    26761 ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta
    26821 tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg
    26881 aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat
    26941 ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag
    27001 attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat
    27061 gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt
    27121 tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca
    27181 ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc
    27241 ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc
    27301 gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt
    27361 caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg
    27421 accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga
    27481 gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa
    27541 gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca
    27601 gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa
    27661 gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc
    27721 tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca
    27781 agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg
    27841 atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca
    27901 cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat
    27961 gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt
    28021 gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag
    28081 tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat
    28141 aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca
    28201 gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta
    28261 aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa
    28321 attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt
    28381 gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt
    28441 cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac
    28501 ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa
    28561 caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa
    28621 gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca
    28681 aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag
    28741 gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc
    28801 ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc
    28861 ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt
    28921 caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt
    28981 tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat
    29041 caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg
    29101 ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag
    29161 tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg
    29221 cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg
    29281 caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt
    29341 tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat
    29401 gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg
    29461 tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac
    29521 aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc
    29581 ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt
    29641 ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa
    29701 tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca
    29761 agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga
    29821 taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc
    29881 agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat
    29941 cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa
    30001 agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc
    30061 aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac
    30121 aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg
    30181 ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa
    30241 gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc
    30301 acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga
    30361 agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga
    30421 agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta
    30481 aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca
    30541 agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga
    30601 agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc
    30661 ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa
    30721 tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag
    30781 taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga
    30841 tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag
    30901 aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc
    30961 actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta
    31021 gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac
    31081 agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac
    31141 aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc
    31201 cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag
    31261 tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag
    31321 ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg
    31381 taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga
    31441 aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa
    31501 atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg
    31561 gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc
    31621 gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc
    31681 gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag
    31741 tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca
    31801 gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag
    31861 aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc
    31921 agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa
    31981 atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac
    32041 aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg
    32101 tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag
    32161 aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa
    32221 aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta
    32281 ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct
    32341 tttggttttc agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa
    32401 agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt
    32461 tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg
    32521 tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt
    32581 ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta
    32641 cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta
    32701 ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg
    32761 gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct
    32821 tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt
    32881 tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct
    32941 tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt
    33001 tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc
    33061 tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt
    33121 tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt
    33181 ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct
    33241 ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc
    33301 caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac
    33361 catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta
    33421 cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa
    33481 gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg
    33541 ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag
    33601 tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc
    33661 gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt
    33721 tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt
    33781 atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata
    33841 cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca
    33901 agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat
    33961 taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg
    34021 gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg
    34081 ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg
    34141 caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat
    34201 taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa
    34261 tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact
    34321 atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc
    34381 gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt
    34441 taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga
    34501 taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa
    34561 cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc
    34621 agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac
    34681 gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc
    34741 taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt
    34801 atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa
    34861 aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga
    34921 agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa
    34981 agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga
    35041 cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct
    35101 tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt
    35161 caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca
    35221 gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt
    35281 tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt
    35341 tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac
    35401 agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc
    35461 gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc
    35521 agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag
    35581 cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc
    35641 gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt
    35701 ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg
    35761 atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag
    35821 ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac
    35881 tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg
    35941 gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga
    36001 gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg
    36061 aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat
    36121 caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat
    36181 atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata
    36241 ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga
    36301 ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg
    36361 agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt
    36421 aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc
    36481 gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata
    36541 tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg
    36601 ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc
    36661 atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg
    36721 agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca
    36781 aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc
    36841 aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac
    36901 ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac
    36961 gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta
    37021 cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat
    37081 caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt
    37141 aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt
    37201 aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt
    37261 gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg tttaactctg
    37321 agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt gtgacttgtg
    37381 accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga caaatcgtgt
    37441 cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat
    37501 catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt
    37561 cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta
    37621 aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc
    37681 gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt
    37741 aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat
    37801 cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt
    37861 tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg
    37921 gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta
    37981 aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt
    38041 cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt
    38101 tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct
    38161 ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt
    38221 ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata
    38281 taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct
    38341 cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt
    38401 ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga
    38461 tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta
    38521 atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta
    38581 attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt
    38641 gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta
    38701 atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact
    38761 ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt
    38821 gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg
    38881 tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga
    38941 tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc
    39001 gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa
    39061 gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc
    39121 catgttctac taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga
    39181 tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt
    39241 tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt
    39301 gggagccata agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt
    39361 tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa
    39421 gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt
    39481 ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt cctaattttt
    39541 ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac
    39601 gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta
    39661 cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca
    39721 tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat
    39781 ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca
    39841 tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt
    39901 ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg
    39961 catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt
    40021 gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc
    40081 aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac
    40141 tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac
    40201 acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat
    40261 tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt
    40321 attacgcggt ggtcgtgtaa gagacttacc aggggtacgt taccatatcg ttcgcggtgc
    40381 tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa
    40441 acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa
    40501 ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca
    40561 aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac
    40621 cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac
    40681 ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac
    40741 gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca
    40801 gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct
    40861 cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca
    40921 aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac
    40981 cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg
    41041 acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga
    41101 atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat
    41161 gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt
    41221 ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt
    41281 attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc
    41341 gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat
    41401 ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg
    41461 cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc
    41521 ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac
    41581 ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca
    41641 atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct
    41701 gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa
    41761 actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta
    41821 aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca
    41881 gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca
    41941 ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt
    42001 cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc
    42061 gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta
    42121 caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac
    42181 tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa
    42241 gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg
    42301 gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat
    42361 aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca
    42421 aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc
    42481 gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct
    42541 taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt
    42601 ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt
    42661 ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt
    42721 gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat
    42781 atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc
    42841 cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt
    42901 gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt
    42961 cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa
    43021 gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct
    43081 gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa
    43141 tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttgaaacc
    43201 ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa
    43261 ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca
    43321 aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac
    43381 cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca
    43441 ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac
    43501 gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac
    43561 gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat
    43621 tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct
    43681 gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt
    43741 gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta
    43801 gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat
    43861 gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa
    43921 aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac
    43981 gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt
    44041 gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt
    44101 attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta
    44161 gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac
    44221 atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct
    44281 gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac
    44341 taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa
    44401 ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca
    44461 atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc
    44521 ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat
    44581 tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat
    44641 tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt
    44701 cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc
    44761 tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga
    44821 cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt
    44881 gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga
    44941 aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg
    45001 tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt
    45061 gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca
    45121 taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat
    45181 tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt
    45241 aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa
    45301 aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat
    45361 tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac
    45421 ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa
    45481 acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt
    45541 caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca
    45601 agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat
    45661 caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc
    45721 tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg
    45781 tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag
    45841 aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa
    45901 atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt
    45961 agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt
    46021 cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt
    46081 aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc
    46141 atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg
    46201 tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa
    46261 agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt
    46321 agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc
    46381 taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc
    46441 tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct
    46501 agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga
    46561 ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca
    46621 atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact
    46681 ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg
    46741 aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc
    46801 aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa
    46861 taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca
    46921 aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat
    46981 caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg
    47041 gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact
    47101 tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc
    47161 gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac
    47221 ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg
    47281 gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga
    47341 aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta
    47401 aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg
    47461 catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta
    47521 aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc
    47581 ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag
    47641 tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag
    47701 acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt
    47761 ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt
    47821 taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt
    47881 cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac
    47941 gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg
    48001 cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg
    48061 aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca
    48121 gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc
    48181 gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa
    48241 gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg
    48301 gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca
    48361 aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa
    48421 aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat
    48481 catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga
    48541 agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt
    48601 tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat
    48661 ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac
    48721 tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt
    48781 agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa
    48841 acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg
    48901 tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct
    48961 tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa
    49021 actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga
    49081 gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc
    49141 ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg
    49201 caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg
    49261 actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat
    49321 actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt
    49381 gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct
    49441 cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt
    49501 gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga
    49561 aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc
    49621 aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat
    49681 caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac
    49741 tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa
    49801 aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa
    49861 ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt
    49921 gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt
    49981 cgaagaagca gttattatct aataacacga gattttccta tatagattga atgcaaaatc
    50041 tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc
    50101 aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa
    50161 catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa
    50221 aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg
    50281 ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg
    50341 tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat
    50401 cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac
    50461 agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa
    50521 agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa
    50581 tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat
    50641 ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg
    50701 agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt
    50761 tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa
    50821 atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca
    50881 aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc
    50941 taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat
    51001 tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc
    51061 aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc
    51121 actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa
    51181 ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga
    51241 taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg
    51301 tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt
    51361 ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta
    51421 tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg
    51481 tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg
    51541 gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt
    51601 gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta
    51661 cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt
    51721 gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt
    51781 aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct
    51841 tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg
    51901 acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt
    51961 atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat
    52021 cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt
    52081 tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa
    52141 tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat
    52201 tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca
    52261 aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt
    52321 attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag taactgttga
    52381 agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt
    52441 agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta
    52501 tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat
    52561 tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac
    52621 atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt
    52681 acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc
    52741 aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga
    52801 acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa
    52861 cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag
    52921 cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag
    52981 tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc
    53041 gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc
    53101 aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa
    53161 aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt
    53221 aggatttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga
    53281 tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat
    53341 cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc
    53401 tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa
    53461 atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca
    53521 attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg
    53581 ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga
    53641 cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca
    53701 gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg
    53761 gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc
    53821 tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg
    53881 tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc
    53941 tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg
    54001 tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga
    54061 tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg
    54121 aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa
    54181 attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg
    54241 tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac
    54301 attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat
    54361 attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc
    54421 aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg
    54481 gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct
    54541 cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact
    54601 cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat
    54661 caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt
    54721 ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa
    54781 ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa
    54841 agtatcagag aaatttatga agattacttt ataaatattg atccttcaga catatggaaa
    54901 tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca
    54961 tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg
    55021 cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt
    55081 gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt
    55141 tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct
    55201 acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac
    55261 cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct
    55321 ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca
    55381 gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca
    55441 caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact
    55501 gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa
    55561 agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat
    55621 gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat
    55681 tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa
    55741 cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga
    55801 tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt
    55861 cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa
    55921 agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt
    55981 cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt
    56041 caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag
    56101 agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga
    56161 accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga
    56221 aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta
    56281 gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca
    56341 gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc
    56401 agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg
    56461 caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt
    56521 ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta
    56581 tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc
    56641 ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg
    56701 tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg
    56761 tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg
    56821 ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa
    56881 cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc
    56941 ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca
    57001 gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg
    57061 aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt
    57121 ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct
    57181 cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca
    57241 gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat
    57301 atccattcta cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta
    57361 gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc
    57421 gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt
    57481 gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct
    57541 acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc
    57601 cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc
    57661 attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa
    57721 accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct
    57781 tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt
    57841 gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat
    57901 caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa
    57961 aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac
    58021 tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg
    58081 tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta
    58141 tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc
    58201 aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg
    58261 tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga
    58321 agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac
    58381 tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat
    58441 gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg
    58501 tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt
    58561 ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt
    58621 aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg
    58681 taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt
    58741 attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc
    58801 agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc
    58861 tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc
    58921 agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata
    58981 cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga
    59041 acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag agtgttatga
    59101 tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg
    59161 ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata
    59221 tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt
    59281 gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat
    59341 tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct
    59401 cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat
    59461 cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg
    59521 gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt
    59581 ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc
    59641 ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga
    59701 aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac
    59761 aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag
    59821 caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca
    59881 aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg
    59941 ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa
    60001 ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac
    60061 tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa
    60121 ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa
    60181 tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta
    60241 tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc
    60301 gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac
    60361 ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga
    60421 agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg
    60481 tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga
    60541 tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc
    60601 aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg
    60661 acttgatccc cagggcagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca
    60721 gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta
    60781 tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca
    60841 aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga
    60901 agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc
    60961 agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc
    61021 gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca
    61081 gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg
    61141 catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag
    61201 gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa
    61261 gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga
    61321 acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc
    61381 ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag
    61441 ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc
    61501 ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt
    61561 ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat
    61621 ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca
    61681 aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg
    61741 ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttaagagga taaacgttca
    61801 gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg caagcagtgt
    61861 atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg
    61921 aaaagaaaga acgaaaaaat tttatagaaa aatcattatt ccagataacc ttttaggaac
    61981 aaaggagaga aaaacatggt acgttacaaa gcaatcattg cgtacgacgg aacaaatttt
    62041 aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta
    62101 caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga
    62161 gtacatgcga tcgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga
    62221 atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt
    62281 gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg
    62341 gatataggga aaccacgcag tccttttcga cgccattatg caagttattt tccttaccca
    62401 cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc
    62461 tctttttgtg catcaggaag ctcgatagag gataaaatac gtacgatcta cgaggctaag
    62521 atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg
    62581 tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct
    62641 gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca
    62701 caccctgaag gactttattt gtatgaagtg aaatatgaat aagaggttat gatagaagtg
    62761 gtcagcttca agaaataaga aggtatttac gaaaattact tctcaaaatt ttgcaaaatt
    62821 tcggcttatt ttcgaagaag ctacttctgg aacaccgttt aaggaagagg tcgtgaaatc
    62881 agcgttttga cctgagtatt agagaaacaa acgggaaaat agctcctcat attttgccgt
    62941 ttgttgagct aataccgaag gtcagctgat tgaacaccgt ttaagaaaga ggtcgtgagc
    63001 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    63061 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    63121 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    63181 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    63241 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    63301 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    63361 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    63421 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    63481 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    63541 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    63601 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    63661 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    63721 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    63781 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    63841 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    63901 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    63961 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    64021 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    64081 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    64141 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    64201 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    64261 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    64321 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    64381 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    64441 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    64501 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    64561 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    64621 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    64681 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacga tcgtcttgtt
    64741 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    64801 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    64861 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    64921 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    64981 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    65041 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    65101 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    65161 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    65221 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    65281 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    65341 gaatccccaa aatcactaat ggaagataaa aaaatgcatc cagccacatg atgatctccg
    65401 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    65461 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    65521 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    65581 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    65641 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    65701 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    65761 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    65821 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    65881 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    65941 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    66001 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    66061 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    66121 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    66181 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    66241 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    66301 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    66361 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    66421 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    66481 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    66541 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    66601 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    66661 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    66721 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    66781 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    66841 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    66901 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    66961 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    67021 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    67081 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    67141 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    67201 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    67261 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    67321 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    67381 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    67441 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    67501 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    67561 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    67621 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    67681 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    67741 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    67801 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    67861 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    67921 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    67981 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    68041 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    68101 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    68161 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    68221 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    68281 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    68341 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    68401 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    68461 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    68521 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    68581 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    68641 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    68701 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    68761 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    68821 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    68881 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    68941 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    69001 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    69061 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    69121 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    69181 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    69241 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    69301 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    69361 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    69421 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    69481 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    69541 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    69601 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    69661 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    69721 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    69781 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    69841 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    69901 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    69961 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    70021 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    70081 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    70141 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    70201 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    70261 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    70321 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    70381 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    70441 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    70501 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    70561 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    70621 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    70681 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    70741 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    70801 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    70861 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    70921 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    70981 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    71041 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    71101 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    71161 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    71221 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    71281 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    71341 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    71401 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    71461 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    71521 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    71581 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    71641 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    71701 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    71761 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    71821 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    71881 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    71941 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    72001 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    72061 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    72121 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    72181 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    72241 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    72301 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    72361 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    72421 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    72481 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    72541 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    72601 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    72661 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    72721 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    72781 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    72841 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    72901 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    72961 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    73021 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    73081 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    73141 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    73201 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    73261 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    73321 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    73381 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    73441 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    73501 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    73561 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    73621 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    73681 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    73741 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    73801 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    73861 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    73921 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    73981 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    74041 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    74101 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    74161 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    74221 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    74281 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    74341 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    74401 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    74461 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    74521 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    74581 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    74641 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    74701 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    74761 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    74821 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    74881 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    74941 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    75001 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    75061 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    75121 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    75181 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    75241 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    75301 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    75361 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    75421 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    75481 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    75541 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    75601 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    75661 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    75721 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    75781 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    75841 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    75901 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    75961 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    76021 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    76081 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    76141 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    76201 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    76261 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    76321 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    76381 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    76441 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    76501 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    76561 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    76621 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    76681 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    76741 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    76801 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    76861 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    76921 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    76981 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    77041 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    77101 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    77161 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    77221 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    77281 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    77341 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    77401 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    77461 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    77521 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    77581 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    77641 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    77701 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    77761 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    77821 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    77881 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    77941 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    78001 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    78061 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    78121 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    78181 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    78241 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    78301 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    78361 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    78421 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    78481 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    78541 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    78601 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    78661 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    78721 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    78781 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    78841 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    78901 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    78961 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    79021 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    79081 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    79141 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    79201 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    79261 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    79321 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    79381 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    79441 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    79501 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    79561 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    79621 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    79681 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    79741 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    79801 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    79861 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    79921 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    79981 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    80041 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    80101 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    80161 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    80221 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    80281 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    80341 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    80401 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    80461 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    80521 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    80581 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    80641 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    80701 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    80761 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    80821 aagcagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    80881 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    80941 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    81001 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    81061 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    81121 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    81181 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    81241 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    81301 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    81361 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    81421 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    81481 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    81541 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    81601 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    81661 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    81721 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    81781 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    81841 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    81901 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    81961 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    82021 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    82081 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    82141 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    82201 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    82261 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    82321 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    82381 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    82441 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    82501 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    82561 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    82621 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    82681 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    82741 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    82801 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    82861 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    82921 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    82981 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    83041 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    83101 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    83161 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    83221 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    83281 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    83341 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    83401 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    83461 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    83521 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    83581 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    83641 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    83701 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    83761 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    83821 gttttgaaac agcgatacga atcaccgaag cattacgtca agaagtgaaa ctcagaaatg
    83881 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    83941 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    84001 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    84061 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    84121 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    84181 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    84241 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    84301 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    84361 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    84421 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    84481 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    84541 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    84601 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    84661 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    84721 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    84781 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    84841 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    84901 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    84961 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    85021 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    85081 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    85141 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    85201 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    85261 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    85321 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    85381 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    85441 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    85501 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    85561 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    85621 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    85681 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    85741 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    85801 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    85861 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    85921 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    85981 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    86041 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    86101 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    86161 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    86221 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    86281 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    86341 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    86401 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    86461 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    86521 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    86581 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    86641 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    86701 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    86761 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    86821 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    86881 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    86941 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    87001 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    87061 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    87121 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    87181 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    87241 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    87301 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    87361 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    87421 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    87481 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    87541 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    87601 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    87661 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    87721 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    87781 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    87841 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    87901 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    87961 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    88021 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    88081 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    88141 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    88201 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    88261 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    88321 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    88381 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    88441 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    88501 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    88561 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    88621 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    88681 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    88741 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    88801 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    88861 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    88921 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    88981 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    89041 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    89101 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    89161 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    89221 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    89281 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    89341 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    89401 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    89461 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    89521 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    89581 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    89641 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    89701 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa gttgactgct tttatcggtc
    89761 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    89821 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    89881 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    89941 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    90001 tcgttcagga atcactggaa cacattgggt tagaagaaat agcggatcaa tcgattgaca
    90061 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    90121 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    90181 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    90241 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    90301 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    90361 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    90421 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    90481 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    90541 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    90601 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    90661 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    90721 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    90781 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    90841 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    90901 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    90961 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    91021 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    91081 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    91141 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    91201 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    91261 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    91321 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    91381 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    91441 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    91501 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    91561 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    91621 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    91681 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    91741 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    91801 ccagcgttct tattactcaa aacaaatcgc agttgtcaca atctcttcta aacgatgttc
    91861 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    91921 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    91981 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    92041 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    92101 gcttactcca ccgttttagt aattgataga aataactctc ttctgctttg attttatact
    92161 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    92221 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    92281 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    92341 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    92401 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    92461 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    92521 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    92581 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    92641 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    92701 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    92761 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    92821 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    92881 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    92941 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    93001 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    93061 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    93121 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    93181 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    93241 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    93301 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    93361 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    93421 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    93481 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    93541 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    93601 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    93661 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    93721 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    93781 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    93841 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    93901 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    93961 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    94021 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    94081 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    94141 aaaaaaaaat ataatataca ttttttattt aattataatt aatattgatt gaagtctagg
    94201 cagtcaag
//
LOCUS       DACCQP010000003        90186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCQP010000003 DACCQP010000000
VERSION     DACCQP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.003"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1156..1554)
                     /locus_tag="IVO87_000205"
     CDS             complement(1156..1554)
                     /locus_tag="IVO87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448437.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1770..2825
                     /locus_tag="IVO87_000206"
     CDS             1770..2825
                     /locus_tag="IVO87_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7448438.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVIEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2845..3198
                     /locus_tag="IVO87_000207"
     CDS             2845..3198
                     /locus_tag="IVO87_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448439.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3380..3676)
                     /locus_tag="IVO87_000208"
     CDS             complement(3380..3676)
                     /locus_tag="IVO87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448440.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3680..4498)
                     /locus_tag="IVO87_000209"
     CDS             complement(3680..4498)
                     /locus_tag="IVO87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7448441.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4724..4945
                     /locus_tag="IVO87_000210"
     CDS             4724..4945
                     /locus_tag="IVO87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7448442.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4947..5213
                     /locus_tag="IVO87_000211"
     CDS             4947..5213
                     /locus_tag="IVO87_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7448443.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5326..5865
                     /locus_tag="IVO87_000212"
     CDS             5326..5865
                     /locus_tag="IVO87_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448444.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="IVO87_000213"
     CDS             6356..6640
                     /locus_tag="IVO87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448445.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6647..6892
                     /locus_tag="IVO87_000214"
     CDS             6647..6892
                     /locus_tag="IVO87_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448446.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6914..7168
                     /locus_tag="IVO87_000215"
     CDS             6914..7168
                     /locus_tag="IVO87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448447.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="IVO87_000216"
     CDS             7250..7435
                     /locus_tag="IVO87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7448448.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="IVO87_000217"
     CDS             7502..7897
                     /locus_tag="IVO87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7448449.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7887..8264
                     /locus_tag="IVO87_000218"
     CDS             7887..8264
                     /locus_tag="IVO87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448450.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8660
                     /locus_tag="IVO87_000219"
     CDS             8265..8660
                     /locus_tag="IVO87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448451.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8966..9364)
                     /locus_tag="IVO87_000220"
     CDS             complement(8966..9364)
                     /locus_tag="IVO87_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7448452.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9560..10321)
                     /locus_tag="IVO87_000221"
     CDS             complement(9560..10321)
                     /locus_tag="IVO87_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7448453.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKN"
     gene            complement(10357..11580)
                     /locus_tag="IVO87_000222"
     CDS             complement(10357..11580)
                     /locus_tag="IVO87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7448454.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKMFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTIKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVSHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12522..13325
                     /locus_tag="IVO87_000223"
     CDS             12522..13325
                     /locus_tag="IVO87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7448455.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13932..14375
                     /locus_tag="IVO87_000224"
     CDS             13932..14375
                     /locus_tag="IVO87_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448456.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGR"
     gene            14494..15552
                     /locus_tag="IVO87_000225"
     CDS             14494..15552
                     /locus_tag="IVO87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7448457.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15594..17543
                     /locus_tag="IVO87_000226"
     CDS             15594..17543
                     /locus_tag="IVO87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7448458.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            17546..21235
                     /locus_tag="IVO87_000227"
     CDS             17546..21235
                     /locus_tag="IVO87_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448459.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVSGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21640..21978
                     /locus_tag="IVO87_000228"
     CDS             21640..21978
                     /locus_tag="IVO87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448460.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            22090..22326
                     /locus_tag="IVO87_000229"
     CDS             22090..22326
                     /locus_tag="IVO87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448461.1"
                     /translation="MDREKMLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKI
                     EKARLETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            22326..24491
                     /locus_tag="IVO87_000230"
     CDS             22326..24491
                     /locus_tag="IVO87_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448462.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGSNTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            24503..24733
                     /locus_tag="IVO87_000231"
     CDS             24503..24733
                     /locus_tag="IVO87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7448463.1"
                     /translation="MREENIVYPVIENQANTKSSQFLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24723..25589
                     /locus_tag="IVO87_000232"
     CDS             24723..25589
                     /locus_tag="IVO87_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448464.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25607..27565
                     /locus_tag="IVO87_000233"
     CDS             25607..27565
                     /locus_tag="IVO87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448465.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYREEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKTGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGEDKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPVDLTNNFVIGEFII"
     gene            27704..28039
                     /locus_tag="IVO87_000234"
     CDS             27704..28039
                     /locus_tag="IVO87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448466.1"
                     /translation="MKQYLNEWKVIEGSLVAQRIKGLPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDEKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            28072..28404
                     /locus_tag="IVO87_000235"
     CDS             28072..28404
                     /locus_tag="IVO87_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448467.1"
                     /translation="MDTAILYINIIPSISFIITLTIFIRRKKFFTEKKLVCFLMKSGV
                     VFLFSWLLLILSTSSFLIILFATVGLIDFGVISGAVMWFWLTRKPKKRNKKNGLENKR
                     LFRLMTNH"
     gene            complement(28565..30160)
                     /locus_tag="IVO87_000236"
     CDS             complement(28565..30160)
                     /locus_tag="IVO87_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448468.1"
                     /translation="MFILDDEINKVKNPRIVPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKSDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFKLLDALIFDNKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KEELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFRYSDKDMEILPWIYDKLNQDQFNKLLYNLNRNNQFYYNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEILNDLKLNLVNK
                     SEQYLGEKINSEEKLLDYIKQIK"
     gene            30281..30865
                     /locus_tag="IVO87_000237"
     CDS             30281..30865
                     /locus_tag="IVO87_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448469.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQIYTTFAGGDLEEKEVDVTHEIGLALSGIQDADVLGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     NTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            30876..31127
                     /locus_tag="IVO87_000238"
     CDS             30876..31127
                     /locus_tag="IVO87_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448470.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            31134..31856
                     /locus_tag="IVO87_000239"
     CDS             31134..31856
                     /locus_tag="IVO87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448471.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYNAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYEQSEPVMDDQSTIK"
     gene            31986..32729
                     /locus_tag="IVO87_000240"
     CDS             31986..32729
                     /locus_tag="IVO87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448472.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            32735..33100
                     /locus_tag="IVO87_000241"
     CDS             32735..33100
                     /locus_tag="IVO87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448473.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKQRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            33116..33646
                     /locus_tag="IVO87_000242"
     CDS             33116..33646
                     /locus_tag="IVO87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448474.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            33808..34173
                     /locus_tag="IVO87_000243"
     CDS             33808..34173
                     /locus_tag="IVO87_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448475.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYLEEKAGVIKDKE"
     gene            34190..34579
                     /locus_tag="IVO87_000244"
     CDS             34190..34579
                     /locus_tag="IVO87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448476.1"
                     /translation="MNEETELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQEFKSEKINLR"
     gene            34609..34833
                     /locus_tag="IVO87_000245"
     CDS             34609..34833
                     /locus_tag="IVO87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448477.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVLETWSDVLLEVTK"
     gene            34886..35167
                     /locus_tag="IVO87_000246"
     CDS             34886..35167
                     /locus_tag="IVO87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448478.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATNDVLVATT
                     EWFIRNNKKLIQFEADENGRHSLFYTNDQKKADRIIAILKENDDGGQRD"
     gene            35148..35390
                     /locus_tag="IVO87_000247"
     CDS             35148..35390
                     /locus_tag="IVO87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448479.1"
                     /translation="MVDKEIKGEIRLFQRENDDWGTEISKNLAEDIELRLSFISMIIE
                     SFAFELGIPQEKLAEILIKASNNSPQHFHIDLGTVN"
     gene            35512..35760
                     /locus_tag="IVO87_000248"
     CDS             35512..35760
                     /locus_tag="IVO87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448480.1"
                     /translation="MSENHLKRLSKGTASMFDANLLIFKSLLEFEKQRVEDIAKYGNY
                     ATVPSVYNDLMKWFKVQTGLEYSQQNLVVKIEEYENHG"
     gene            36035..36247
                     /locus_tag="IVO87_000249"
     CDS             36035..36247
                     /locus_tag="IVO87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448481.1"
                     /translation="MPVKTNTEWLDICDELFDTKDKKELAMRVAYAIVQKDKYFAVPS
                     TDYDIEVIVDPITGDKKLRVVIECNA"
     gene            36356..36532
                     /locus_tag="IVO87_000250"
     CDS             36356..36532
                     /locus_tag="IVO87_000250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448482.1"
                     /translation="MDIFEVKPDSGKFIVLQEKRVIAIERTEIEAYKKIHELAEQYIE
                     DLNNNFQEFLTQNE"
     gene            36643..36864
                     /locus_tag="IVO87_000251"
     CDS             36643..36864
                     /locus_tag="IVO87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448483.1"
                     /translation="MDIYEEVICDVEGKEEFLPCIKELETTVAITKVANAVSNETNEL
                     VGIHEAGNLVFSCLNSNKSALLALDGDLF"
     gene            37236..39476
                     /locus_tag="IVO87_000252"
     CDS             37236..39476
                     /locus_tag="IVO87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448484.1"
                     /translation="MSEFEFLDKSIGSAFLIDPDLFGAWAQSSGYNWAFSDDMVELLS
                     QTMNWEAYKIKQFLDVVAQPIYTSADDHWSGRELTTLKELAEYNKRYKIPFQVSYMSD
                     DFSVIYSIGKESRYLIPNRHLEIKMNSDYTDYTLSELKALRQSDSEEFSALKNEVSAD
                     NFRRSDIEELKKKQKQQLEKQKKLIEDIEYKRVEELAIQEAKIEKMRQELYKKQQSML
                     AEANKMRFELEKKVEDLNTKIFYLETEINAIEGYFGESTTFVQLRSGQKSLPEEPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENVYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELEYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            39614..40411
                     /locus_tag="IVO87_000253"
     CDS             39614..40411
                     /locus_tag="IVO87_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448485.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLGYSKEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            40451..40633
                     /locus_tag="IVO87_000254"
     CDS             40451..40633
                     /locus_tag="IVO87_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448486.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            40677..40964
                     /locus_tag="IVO87_000255"
     CDS             40677..40964
                     /locus_tag="IVO87_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448487.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            41001..41288
                     /locus_tag="IVO87_000256"
     CDS             41001..41288
                     /locus_tag="IVO87_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448488.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            41379..41618
                     /locus_tag="IVO87_000257"
     CDS             41379..41618
                     /locus_tag="IVO87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448489.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            41746..42075
                     /locus_tag="IVO87_000258"
     CDS             41746..42075
                     /locus_tag="IVO87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448490.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTYFYIGDRE
                     KYYDPSL"
     gene            42128..42976
                     /locus_tag="IVO87_000259"
     CDS             42128..42976
                     /locus_tag="IVO87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448491.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            42973..43290
                     /locus_tag="IVO87_000260"
     CDS             42973..43290
                     /locus_tag="IVO87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448492.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            43293..43526
                     /locus_tag="IVO87_000261"
     CDS             43293..43526
                     /locus_tag="IVO87_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448493.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIFQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            complement(43643..44122)
                     /locus_tag="IVO87_000262"
     CDS             complement(43643..44122)
                     /locus_tag="IVO87_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448494.1"
                     /translation="MTDKTSDLPKLAPKTSRVKRKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            44242..45579
                     /locus_tag="IVO87_000263"
     CDS             44242..45579
                     /locus_tag="IVO87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7448495.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNWKQESGNDPRTVEG
                     YYGDGQREVEFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGGAWWTTEAQLDFMF
                     KGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAVRGGNAKEIWEYMK
                     SKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTDDANSGSGSMGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            45602..46129
                     /locus_tag="IVO87_000264"
     CDS             45602..46129
                     /locus_tag="IVO87_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448496.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            46116..46679
                     /locus_tag="IVO87_000265"
     CDS             46116..46679
                     /locus_tag="IVO87_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448497.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLENLDSYVRAE"
     gene            47102..47839
                     /locus_tag="IVO87_000266"
     CDS             47102..47839
                     /locus_tag="IVO87_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448498.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(47856..48272)
                     /locus_tag="IVO87_000267"
     CDS             complement(47856..48272)
                     /locus_tag="IVO87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448499.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            48664..49911
                     /locus_tag="IVO87_000268"
     CDS             48664..49911
                     /locus_tag="IVO87_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP7448500.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            50181..52571
                     /locus_tag="IVO87_000269"
     CDS             50181..52571
                     /locus_tag="IVO87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7448501.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            52620..54455
                     /locus_tag="IVO87_000270"
     CDS             52620..54455
                     /locus_tag="IVO87_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7448502.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            54530..55387
                     /locus_tag="IVO87_000271"
     CDS             54530..55387
                     /locus_tag="IVO87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7448503.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            55377..56486
                     /locus_tag="IVO87_000272"
     CDS             55377..56486
                     /locus_tag="IVO87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7448504.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            56825..57625
                     /locus_tag="IVO87_000273"
     CDS             56825..57625
                     /locus_tag="IVO87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448505.1"
                     /translation="MVEISKTTLDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            57713..58519
                     /locus_tag="IVO87_000274"
     CDS             57713..58519
                     /locus_tag="IVO87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448506.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            58751..59620
                     /locus_tag="IVO87_000275"
     CDS             58751..59620
                     /locus_tag="IVO87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7448507.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            59640..59987
                     /locus_tag="IVO87_000276"
     CDS             59640..59987
                     /locus_tag="IVO87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448508.1"
                     /translation="MEIISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            60000..61262
                     /locus_tag="IVO87_000277"
     CDS             60000..61262
                     /locus_tag="IVO87_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448509.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            61342..62718
                     /locus_tag="IVO87_000278"
     CDS             61342..62718
                     /locus_tag="IVO87_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7448510.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            62715..63344
                     /locus_tag="IVO87_000279"
     CDS             62715..63344
                     /locus_tag="IVO87_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448511.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            63363..63842
                     /locus_tag="IVO87_000280"
     CDS             63363..63842
                     /locus_tag="IVO87_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448512.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            64344..64397
                     /locus_tag="IVO87_000281"
                     /pseudo
     CDS             64344..64397
                     /locus_tag="IVO87_000281"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            64401..65000
                     /locus_tag="IVO87_000282"
     CDS             64401..65000
                     /locus_tag="IVO87_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7448513.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIHFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            65016..65492
                     /locus_tag="IVO87_000283"
     CDS             65016..65492
                     /locus_tag="IVO87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448514.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            65509..66411
                     /locus_tag="IVO87_000284"
     CDS             65509..66411
                     /locus_tag="IVO87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448515.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            66484..66678
                     /locus_tag="IVO87_000285"
     CDS             66484..66678
                     /locus_tag="IVO87_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448516.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            66783..67211
                     /locus_tag="IVO87_000286"
     CDS             66783..67211
                     /locus_tag="IVO87_000286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448517.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNKETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            67446..68033
                     /locus_tag="IVO87_000287"
     CDS             67446..68033
                     /locus_tag="IVO87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448518.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            68033..68932
                     /locus_tag="IVO87_000288"
     CDS             68033..68932
                     /locus_tag="IVO87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7448519.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            68932..69846
                     /locus_tag="IVO87_000289"
     CDS             68932..69846
                     /locus_tag="IVO87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448520.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESGMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            69898..70113
                     /locus_tag="IVO87_000290"
     CDS             69898..70113
                     /locus_tag="IVO87_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448521.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANRLY"
     gene            70303..70557
                     /locus_tag="IVO87_000291"
     CDS             70303..70557
                     /locus_tag="IVO87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP7448522.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            70736..71785
                     /locus_tag="IVO87_000292"
     CDS             70736..71785
                     /locus_tag="IVO87_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7448523.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            71964..72194
                     /locus_tag="IVO87_000293"
     CDS             71964..72194
                     /locus_tag="IVO87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448524.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            72197..72454
                     /locus_tag="IVO87_000294"
     CDS             72197..72454
                     /locus_tag="IVO87_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448525.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            72671..73546
                     /locus_tag="IVO87_000295"
     CDS             72671..73546
                     /locus_tag="IVO87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448526.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQENLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSEKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            73556..74350
                     /locus_tag="IVO87_000296"
     CDS             73556..74350
                     /locus_tag="IVO87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448527.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            74379..75497
                     /locus_tag="IVO87_000297"
     CDS             74379..75497
                     /locus_tag="IVO87_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448528.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKVKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTVNEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFAKSKDEVKGVVIDDDFEFDFD"
     gene            75530..75850
                     /locus_tag="IVO87_000298"
     CDS             75530..75850
                     /locus_tag="IVO87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448529.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            76107..76796
                     /locus_tag="IVO87_000299"
                     /pseudo
     CDS             76107..76796
                     /locus_tag="IVO87_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
     gene            76797..77000
                     /locus_tag="IVO87_000300"
     CDS             76797..77000
                     /locus_tag="IVO87_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448530.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            77020..78648
                     /locus_tag="IVO87_000301"
     CDS             77020..78648
                     /locus_tag="IVO87_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448531.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            78699..79067
                     /locus_tag="IVO87_000302"
                     /pseudo
     CDS             78699..79067
                     /locus_tag="IVO87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            79167..79367
                     /locus_tag="IVO87_000303"
     CDS             79167..79367
                     /locus_tag="IVO87_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448532.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            79501..80727
                     /locus_tag="IVO87_000304"
     CDS             79501..80727
                     /locus_tag="IVO87_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448533.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            81577..82902
                     /locus_tag="IVO87_000305"
     CDS             81577..82902
                     /locus_tag="IVO87_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7448534.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            82895..83242
                     /locus_tag="IVO87_000306"
     CDS             82895..83242
                     /locus_tag="IVO87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448535.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            83593..84414
                     /locus_tag="IVO87_000307"
     CDS             83593..84414
                     /locus_tag="IVO87_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7448536.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            84434..84994
                     /locus_tag="IVO87_000308"
     CDS             84434..84994
                     /locus_tag="IVO87_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7448537.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            85960..86727
                     /locus_tag="IVO87_000309"
     CDS             85960..86727
                     /locus_tag="IVO87_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7448538.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            86753..87298
                     /locus_tag="IVO87_000310"
     CDS             86753..87298
                     /locus_tag="IVO87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448539.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFDGQQIGLKKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            87299..87865
                     /locus_tag="IVO87_000311"
     CDS             87299..87865
                     /locus_tag="IVO87_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448540.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            87880..88128
                     /locus_tag="IVO87_000312"
     CDS             87880..88128
                     /locus_tag="IVO87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448541.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            88148..88387
                     /locus_tag="IVO87_000313"
     CDS             88148..88387
                     /locus_tag="IVO87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448542.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEQLLRKAQQKGYPDKMLKQIGYYT
                     PEERIEKESCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            88404..88988
                     /locus_tag="IVO87_000314"
     CDS             88404..88988
                     /locus_tag="IVO87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448543.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQSILCSEQDSKRLYGLYDLQLNGLKAQYV
                     ADSFDDMVGQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSIVES"
     gene            89095..89376
                     /locus_tag="IVO87_000315"
     CDS             89095..89376
                     /locus_tag="IVO87_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7448544.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            89366..89707
                     /locus_tag="IVO87_000316"
     CDS             89366..89707
                     /locus_tag="IVO87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7448545.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWVDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT        33456 a        12592 c        17569 g        26569 t
ORIGIN      
        1 acttttgttt tgtgttaagt ttctttacat tcctacgccc tttgtgtgta aaatttcttt
       61 attcctccca cgctccgtgt tattttatat ttcctttttc atgttatttt tcgtcagaaa
      121 ccgatctagt ttttacctat tcccaaaatc catttattcg ttatttttta acctgattaa
      181 ggataattat ttgatgctta aattagctaa aaaggcgatt agttaccgat ataatcaagg
      241 tattaaagct gaattaggcg gaaaggcaaa atgttcgcaa aatcaaagta tcaattatat
      301 tgttttttta taaatttgta agtgaaaatc tgaaaaatcc tctttaaatt ggtataggca
      361 ttaaaattac atacaaccac tcttaaaagt atgcgctttc atcgttttat atgatattaa
      421 tatcaataaa taacgatata aaaaccattt atcataatgt tttttaagga tataaattga
      481 tctaatttaa aaaaataatt caaaatttaa aagttttaat attacaagta aaattatgtt
      541 atcgtaaaat ggtataaaat agcttaatat tctatttttg ttattttatt agcaggaaaa
      601 tctaaatatt tttacaagaa atcgtagaaa tatattggtt tcgccttcag attaatactg
      661 gatcatatta atctgaatac aatatatgaa ctaataaata gcttaacctc gctattttgt
      721 tttttctgat cattatatac acagcattaa caaatagaat tttaaaaata aaaaaagtaa
      781 gacaaaaaaa gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt
      841 tgtcctttaa aaagcactga tttttaaatt gcgttatttt ataacccgaa tacagtctgc
      901 ttgttaggca ttgtccttaa attacatttt tcataggtta tttaatcatt taatctgttg
      961 attattcttg atctaaaact tagcttaaca aatatataat cagccaaccg cctgataaaa
     1021 atgcgatcat tgctaatata taagcaaaga taaaaagatg ttcgatcagc ttaaaccctg
     1081 atgcgattaa gcagaccaag taaaatagaa attctcttac tgataatggt aattttctaa
     1141 ctagcatact cataattaac accccaaagt aatattgtat tttgaacatt ttacatccag
     1201 tacagcatca atcaaccatt gccaatcaaa aatacaaggc ttcattgaag tatgtttttc
     1261 agatgaaaag atataatata attcctttgg aataatattt tcagttgttg ggatcaagct
     1321 aaatctaatt ccatctaact catagctacc agctttctct ccattaaaaa tttccttagc
     1381 actttcttct aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga
     1441 agcatttccc ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc
     1501 agtaaaatct tctaaaataa tggtagtgta attcttattt cttctcaatc gcatcaggat
     1561 cactccttaa tataatttat gtttttatga tatcataata agataaattt gcaagtattt
     1621 gtttacacct ttttatttac actttattat aattaattat acttttatga ttgcattttt
     1681 ttcttataaa cagttgcaac taatagtaaa taggtgtaaa ctattttcgt accgaaatac
     1741 cataaacaca aacagaaaga ggtcatacca tgaaagaacc attattgttt tccgttgact
     1801 tagggaataa atcagtaaaa gaaatgagta cacacgtttc tccactctca cttccctctc
     1861 gtattttgaa caaaaatatc attaattcag gacgtttttc taatataggt tcctcattaa
     1921 aacgatcact aaacgtttct gaatatagcc tagtaaacac tactgaaagc tattattttg
     1981 ggaaggatat acacgagcta ggaaaagatg actacgtgat agaatcacta ggttttggga
     2041 ttagcagata caagcaggaa acctaccaaa atatcctatc tttctcttta gctaacctag
     2101 cacgtttaga agatcaatct gatgacgttt taaccgtcaa tctagtttta ggtctgcctt
     2161 catccgacta taccgaaaca atcgtcaaat acgtgatgca acaagccaca cgacaacatt
     2221 cagttgaagt tgacggtaag gcttataata taaacgtaga taccgtttgg gtactgccac
     2281 agccaatcgg aacgctatat aacctcctac ttgctgatga tggtagcgta caagatgaaa
     2341 ccttgatcag tcagaagatc ggttttgttg atattggtgg tggcacgaaa ctttttgatg
     2401 ttgtccaaaa ttttgaaatt gacgaaaaca atcgtgaaca aaaggatacc ggcattttta
     2461 ctttatataa tcgtatcgta aatatgcttg accttccgat cagtcctaat gctttccaac
     2521 ttgaaaagat tatccgagaa ggcaaagaaa aaggcgtata tctatttaaa ccaagcggaa
     2581 atgaaagttt tgatatttca gaagtaattg aaagagaaat aaaaacatac acaaatatgg
     2641 ttgttgcaga agttttagct acattcaaaa acttcacgac cttagataaa gttgtcttta
     2701 ctggtggtgg agcttctatt atcgacaaac aacttgtaaa agataaaatt ccgcatacct
     2761 tgtttgttga agacagtgaa ttttctaatg taaaaggctt ttacaagttt ggtttaactc
     2821 aataaaacag cgaaggagat caacatgaat aagggaaaat cagagcgtta tgtagttata
     2881 atcaatagag aaaaggttag cgagttacct ttagcagagt ttcttgaatc cgaatatgaa
     2941 acactaggcg ttaaatactc tcagacgatc aaaaaagctt tgaacctata tcggcgtgta
     3001 aagaacaatg aagttacaat tattgaagca ggcaacgata gaaacgatag caaagtagca
     3061 gaagtaaaga ataaaccaac tgatcataac cctattcaac aagagcatcc ttcaacggtt
     3121 acatccgatc attcagcaaa gaaagaaaag ccaatgctaa aagggttcaa tgcaccaagt
     3181 aacaaagact tactttaaaa tgaaaaaggc gaggttacaa tacctagcct ttttattatg
     3241 tacaaatata aacataaaca tatacgtata catgtatata aacatgtata cgtatatatc
     3301 cataaacacg tatatgaaat ttagttaagc tatacctaac cgcttattcc aataaaaaaa
     3361 gtgatcagct ttaaactgat cactcaccaa aaacttcatc atacaagtta tctatcattt
     3421 caccaagtaa agctgaatct gatcgaattc ctaacttttc gctgatttta gatagcttct
     3481 ttctttgcga tggttgaagc gtgaattgat aatttttctt cttttcagaa ctatcacttt
     3541 ttagtttagc taccaaagaa cttgttgttt ctttaggtgt ttgtttaggt tcttcaactg
     3601 cttcaaccac ttcaggaact tcttctactt cagcttcagc gaatttttta aatctatttt
     3661 ctttcacttc aatcactcct tattcttgtg ccgtatcaat tatcttgttg tatagcgtaa
     3721 acgtgctttc aaagaaatcc ttattcttac gatagatatt cttatcctta gcgaactcag
     3781 caatattctt accttctaag ctagttgcat taagcaattc cttctcttgt acatatccaa
     3841 gataattaga taacgttggt accaattcta ggaactcacg acttgaatta gtattatgtt
     3901 tgatcctgct accgatcgta gcgtatttag tttccatcag tgtttcacca gtcttaatat
     3961 caatcaactg ttttgctagt gcatcaatat ctttaccaag cttcagcaaa gcttcaaaac
     4021 catctttaga tggatcagaa atgcctacga caagatcaga tacagcccaa gcattttgag
     4081 ctagtagacc tgcatcatta tgagtatcaa tcagaattag atcatacttc tctttaagct
     4141 gttcatagtt aacaacgatc catctaaaca gtagggttct gttgttaggc atatcacgca
     4201 tttgcatctc aacttgtttt aaatcttcac ctgctactaa aatatccatg ttctcattta
     4261 tagctaaagg ttcagctgat ccatgaagat ataaatgatt gattgtattc tctcttttcg
     4321 ctttactgaa agcatcagga aagcgttttg ataggttttg cgacttatct tcatcaatat
     4381 acagcacctt catacctaaa ttttcgacag catatccacc tagattgtag attgttgttg
     4441 ttttacctgt tccacctttt tcaatgtttc cagtaatgat tgacaacttc ttttccatga
     4501 ttaaacctcc cttattaacc ttttgtaact ccattataca ctataaacac gtttatgtat
     4561 atgtttatat acataaacgt gtttatattt atatttgctt ttatattcta taacataaac
     4621 atatatatgt atatgtttat gtttatgtat atgttcatat atataactat tgcatctgtc
     4681 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
     4741 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
     4801 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
     4861 cacctacaaa gaactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
     4921 tatgctaaaa gagttaggct tataagatga gttaccaagt aagatatgaa cgtgaagctc
     4981 aaaaaacgct aaaaaaaatg gataagtttc aggcaaaggt tattctaaac tggatcgaga
     5041 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
     5101 caggctactg gcgatatagg gtaggtgcat accgtataat agctgatatt agcgagcatg
     5161 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
     5221 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
     5281 ttacctaaaa aagaacaata tcctcttgct attatacaca ccagtgtgta taatataagt
     5341 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgcttat
     5401 tcatcagcaa atgaagtaat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
     5461 gtagatacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
     5521 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
     5581 gacagtcctg cttactttaa gaaaattcca aaaaaatggt tgaaattctt tgtcggtgat
     5641 caagaaaaaa tggttaagcg acaactagat agaattaaag aagaaagcaa taaaccaaaa
     5701 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     5761 gtttatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     5821 cggttcactc gactatcagc aggagatgtt aaggaagtag cctaaaactt aaatacataa
     5881 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatatcaac gtattaggta
     5941 cgaacgcttt taaatgcttt tctgggcttt gattttaggg tagggctaaa acatcaccat
     6001 ctgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactaa taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 aacggactag ccgtttcatc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacattg
     6301 gtgtgtataa tgtaattata aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aaatttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tcgggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tggatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttggct tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg aggggagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgcttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt ttttataagt
     8461 cagattgtta taatcaacac ttagttttag ggagtttttt gcttatctac accattttga
     8521 tgcttaattt atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga
     8581 tcgagagaaa catcaaaagg gtatctttca agatcacgtt aaacaagcct accgataaaa
     8641 agctctcaga agcgttctga gagcttttga cattttcttg tataatttat ccaaacacat
     8701 tacaaagcac ctaattcgag cttatatcaa gcaaatttgc tatttattcc tcaatacata
     8761 tctaaaaatt acttgatgga aaactagata catggtaagc atgaaaagaa aatcaatttg
     8821 agcagatgca gtataataca tagcaattta aaagctctca gaacgcttct gagagctttt
     8881 aatatattct ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt
     8941 gcttaatatg atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag
     9001 ctttcgacag attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca
     9061 ctgattttcg atcgtaattt tgcttgtttc gagctttagc aactttcgta ttagctttta
     9121 cgacaaataa attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg
     9181 taggcgatgg aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt
     9241 cttgcgccat ctcaaaagca tcttctaaag tttcaccatg cgtatataag tctggaaaat
     9301 ctgggaattt aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt
     9361 tcatcgcatt gcacctctct aattaaaaaa taatttttgc tttttagttt caaaaaaaga
     9421 atcgcctacc aaaaaaattg ataggcgatc acaagctaat taacctctaa aggagattaa
     9481 taccttgctc ccaaggcact acatatctta gcatacgtct gaagcatctt caaacgagta
     9541 agatatattt tgcattaacc taattttttt ctctataagg tttttcatca aactcactag
     9601 aaatattaaa ctggacagca aaattgtaat ttctgccttt tctaacttta gcaaaatcaa
     9661 tcttcagcat tgatttttca ttaatttcct caactgattc ccttaaaaca cgtctatcaa
     9721 aattcgatat tgttttgtac tcattttctg ttttcgtaat ctcacgaatt ttaactattt
     9781 caatcagggg attcttatat atttcagctt tctcaaaatc tcctttattt ttggcgataa
     9841 gatatttttt ataatacatc gccaaatatt tgtataacac aatgctatat tttttttgaa
     9901 agcttgctaa atcttgaaaa acaaaatatg gatatttctt tttgattgaa agaattaggg
     9961 gaattacttc ttgcgagaaa ttgaaagtta taatttgttc ttctaagtcc caatgaattt
    10021 gatcaatagc agatactgta gcataagtct tatattcttt atctaagttc gatatttcat
    10081 aaactgcttg tctctgaagg gtgttaacta tattttttag ttctgtttca agattttgat
    10141 caacatccat gtatttgatt attgctttaa caggtaaaac tatttcccct gttttcttta
    10201 atttgttagg agagatataa ctgaacagca attgataaag ttcatacgac tttaaatcca
    10261 ttttcatgtc aattgttgct aagaactcct cgttgtactt tgtcagttca tcttttttca
    10321 aaagcttttc cttccttcac gcaagttttt cagttgttaa tcataaaggt tcacataagg
    10381 taaataactc gtaattgatt tttcgagata tttaacagta ttttttattg tgtaatcctc
    10441 cttatgagta gcaacgtaag atagatgact gacaactgat tcaaatcctc tatctttgga
    10501 taaacgatca taaagcggat aaactttatt ctgaagccga ctcataaatt tcagatcgca
    10561 taagtcggca gaactgatca aaaagttttt gattagtaat ttagtgtatt tgctattcat
    10621 agctttcaag tacgattgat tcattttctc tgttttatct tctgcacctt ttaaataagc
    10681 agggtctctt tgttcttgct tataaggtac cggtactgac atggtacgtg taagcaagaa
    10741 ctttatatct gtataagtat gacccttctt gataagctca tagtcaattt taaaatcagt
    10801 gtatttattc agttgggcaa tcggttcttt aattacacgt ctttcaaagt ctcgaaaagc
    10861 tttatatttt ttctcaatac tgaatttctt tcgtaactct gcaattgaaa aaacggggtt
    10921 ttttaatcta tcaagtgatt ttgtactcca tgttaagtta tctctatttg cttcatacag
    10981 attaaaatct ttagtgaata attgaaaaaa actgatcgca tatttgcttg tcaagttaac
    11041 tacttgatca aatgtatatt gtgtaaaatg attttctaag ttcgttaaca aaggcataat
    11101 tgaactacta aatttaatag atactgcatc agaaaaatca ttgaattcag taatttctag
    11161 cggtgagatg actttgtatt cagtcttacc gttaggttta gttctttgaa ttttaatgac
    11221 agattgctga tgtaattttt caataacttg ttttaatctt gaatgttttg aagaagatga
    11281 actagaagac atcaacgcaa ataaagcctt tttagatact ttgatggtat tattaaaagg
    11341 cggattttta gggtcaagat gtcctatagc gtaaagataa attttttgtg gcatagcatc
    11401 catttcaaca atgctatcca caagttgatt cgataaagta acagttaaat caaacatttt
    11461 tagattgctt tcggtaggtt caaatttcgg gtttacattc ttttctgtta ttttcatacg
    11521 attgcctctt ctttctaagc ttggtacgag cttaaaaact tggaactggt acgattccat
    11581 tgaataagta tagacctgtt ttcgatacga tacaataaga aatgtatcgt aaatttataa
    11641 cataactcaa tctcagtaaa acgcatttta tttttattaa tcaaatttca ttgataagac
    11701 agcagaaatc gctattttag aacctttaca aaataaaaat ccttttcaaa aaaaatacgt
    11761 aattataaaa attcacaaaa ttaaaaaaca aatatttttt tagtttttag aaataaattc
    11821 acatactttg tctttttttg tatatattcg gaataccaca tcgctttttg tccatgttcg
    11881 catcgcttta tgtccaccct aaaatctatt taagattgat ataaagcctt tttggagatt
    11941 gctaaagttt taaagattaa aatatatatt ataaatataa ataaaagggg aagctgaagc
    12001 ttcctccttt tccttttttt taattattca aaattacaca agcaaaataa actgctagat
    12061 agtgttagtt ttggttttaa ggtttttaat ttttgagatt ttgaaataag agaggaggaa
    12121 tcctccttta ttaaatatct gttaatatat ctgttaaata tctgttaata tatctgttag
    12181 gggggtctta accccttggg agagtaagta ttgaaggggt atgaaggtaa cacttttgga
    12241 cagtaaaggt aacaaaagtg gacagtaagg gtaacatttt tggacagtaa aggtaacaaa
    12301 agtggacatt tgaggtatca aatcaggata agacgttacc ttcggttttc ttgatttgaa
    12361 aatcgaaaaa gtagaaaact agattagtaa caaatctttg ttgactgtta cctttagttg
    12421 tgatttaatt taggtaacaa gcagaaaaaa agcgtttcct ttaatttagt accaaattaa
    12481 catgattaaa gggatgcaac aagaagattg gagagtaatc aatgtctcaa gaagttaccg
    12541 aaaacgttat tgataatgat gtagatttag tatcagatca aacaaatgca attgtgcgtt
    12601 ataacaatgg tttgaacaca acagctttgc gaggatttaa acctgtcgaa atggatgtgt
    12661 tttgggctat tttatcaaaa atgaaacgtc aaggaacagc agaagtaact tttgatttca
    12721 acgtctttcg ttatttggtc aaatacgaca gaagagaaaa ggacagtttt tattctgtgt
    12781 taaaagcttt aagtgacaaa ctaggtactt taacctaccg ctttgaagat tcagatgtct
    12841 atgaacagct gtggcttttt caaaaattcc gaatagataa acagcaagaa acagttacaa
    12901 tcaaggcaaa tgaggagttt gaatttatct taaattcaat tggcaaaaac tttactcgtt
    12961 ttgaacttga taactttaca aaactaagaa gtagctacac aaaagagctg tatcgtcatc
    13021 tcatgcaata tagaaataaa gagtggcgaa atggctattg ggtagtcaaa gttgaagatt
    13081 ttagaagagc gttaagtatt ccttcaagtt acagaatgac aaatattgac aaaagaatat
    13141 tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa tggtatacag gtatttgaat
    13201 cttttgatta tgaaaaaatc tatgcgaaga aaggaagaaa agttgatcgg ctcagattca
    13261 cttttagtga accagaatca aacttgccat ccatttcaat gcataattgg ttagaagaag
    13321 actgaaaaga agcaggttgt aattttaata caacctgctt cttttgaaaa aagtatgggt
    13381 aacacttttg gacaataaat gcaagaagtt tttttgtgac caaactttat attagattcc
    13441 tgtgattttg tgtgttagaa aagccctgat agaaaagcaa attcaaacac tttttaatat
    13501 gtatgggtaa cacttttgga catgtcaata ataaatatat tttctttcgt cttttgccta
    13561 actaaaggta acacttttgg acagtatagt tttgtaaact aattagatcg ggtttaatga
    13621 gatatatctt agcatttatg taagaatgaa ggtaacatta atggacatgt caactgtttg
    13681 aacaaaaaaa tatagactat ttggatctga tttttcattt ttccaaacgt taaaaaatag
    13741 ttagttattt tattaataga tagtcatcct taatgtttga aatagcagaa aataagatat
    13801 tgtttgataa atgttgttgt tataaggaaa acttgaattt gttaattgat ttatttatgt
    13861 tattatgata ctataataac ataaatgtgt tacgcccgaa cctcatttta caaaaatgaa
    13921 ggggtgagac aatggatgct attaattggc atgaatttgt taactatatg gagaaactac
    13981 tagatgatct gaaaagtttt agcattgctg atgccgtaaa acatgggttg ccagttagta
    14041 caacgatgaa aatgcgaggg ttgaaggttc cggatgaaat tatcaagctg aggactaaaa
    14101 caatcattga aactgcaaaa attgttgaca gtatgaacct tgatttgaaa ggcaaaaaaa
    14161 caacttatga gaaagacttt gtacgttttt taaaaaatgt tatcggaaaa tatctaggga
    14221 aaaagtcttt tactgaattg agagacgaag agattgtacc aactccaatg gttagcaaaa
    14281 ttcgcaaaaa caaagccgag ggaaattact atcttggcac aatagcaaaa atcgttgagt
    14341 atttggaaaa taaagagcaa aagaaaggaa gatagttcgt gtgataaata tgaataggga
    14401 agaatgtacc gaaatgataa aaaaacccga acctagaatt accgaaaaac tgactgattg
    14461 cttgctcttt tacgaaaggg ggcaggcaaa ctaatggaaa aagatgtcaa ttcagcatcc
    14521 actaaagatc aatacaatat cattggagga ttacttggtg ataaggataa gagaacacgt
    14581 catggattac gcattatgct tgcgacattt ctaggggtgt ttcttttatt aacaggcttg
    14641 ggtagctaca tttatcaatt aggaaactat aattattatc ttgtcaacaa tacaacacca
    14701 atcggctcat cattgacgtt tgctcgatca ggagcagaga tcactatctc agatgtttgg
    14761 acagataaaa ctagagatgt aacggtggtc aaacttggtt actcaggaaa cgcaagaaaa
    14821 ttgctatcta cacaaggtaa aaattataac ttgtatatgg caacaaaaga aaaagatcgt
    14881 cctaagaagc ttgaaatgag ttatgggatg ttaagtaccg atggtgatgc tttcttgttc
    14941 ctaaaaggta aattagatga aagagcttat caagttttta ttgctaacca agtatcacta
    15001 atttcaggtg attatacagc tgatgatgat accaaagtca atgtgaatga atcagattca
    15061 gtcaccaagg cactttctca gtattctatg aatgatgttg ataatgaagg agtaatttct
    15121 tttgggaaaa aagcaaaacc agttaatact cctgataata tcaatttcag aataaatcca
    15181 tactcagata caactaacgt atatcaagga agcttcctta ccaaagatgg cgatattgat
    15241 tactcaaaag ttgttgctgt gacaagtaag aataatgttg taaataagct agaagaccag
    15301 ttaaatgctt caaatactaa catcaagaaa atcgaagcca caaaagctga gtatcttgaa
    15361 cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt
    15421 cgaatggaga ccgaaattaa aacaatcaat gatacacaaa aattgattga tcagtacaaa
    15481 aatgctgact ttaacaaaga ttcttttgga gatatgaaga caacatttaa gtacctaacc
    15541 ctgactgact agaaacaaat tattcttatt ttagaggaag tgaacacaaa cagatggaaa
    15601 atagtaccga gttctttaaa aatattcaaa ccgaattcaa tcatgctctc acactaatga
    15661 aaccaccgaa acataagtta gtggggcgtg atgacgaact ccatgagttg tcactgatct
    15721 taaatagacc ggaaacacca caagctatct tgttaggtga agcaggaaca ggtaaaacag
    15781 cactggcaag tgaatgggct aaaagacagc gacaagaggg gaagaaagta caagtatata
    15841 aattatcaat cggacaattg cgagttggag gagatgacgt cctccaaaca cgtttggaaa
    15901 ctataattcc aagattgaag gaatacgaac gaattttaaa atccgaggac aaagataatg
    15961 aatttgtcct ctttatagat gaatttcata aagttgtcag ttcatttggg agcggtaata
    16021 aaattggggg agattcgctg aaggaacctt tagcaaatga atcacttcag ttgataaccg
    16081 ctacaactag aaaagaatat gaaaactata tcaaacgaga tgatgcttta ggtagacgac
    16141 tacaaccgtt agaactcaac gaattaaaaa gtgatgtcgt tgtcgatata ctaagacaat
    16201 ggttgaatac atcatctgtc aaagatgatc ctgaagcttc cttgaaatta tccaaaaaga
    16261 ttcctgatga attgcttgaa gaaatcgtac aagcaaacaa gctttatcgt gaggatttat
    16321 ccgaaccggc taaatcaatt gccgtaatgg aagatttgat tgcgaaggat cgtgtagatg
    16381 gcaatgcgta taatcgagaa agcctgttac aaatatttgg aaaatataat attcaactag
    16441 attctgattt tgatatagca aaattggaag aggagctaaa gtccattaaa gggcaaccag
    16501 tagcggtata tacggtcacg atgttgttta aacgaatgaa acttggattt gatcgtttta
    16561 aagggcgaaa gcaaccaaaa gcaacggctt tgtttacagg atcaacaggg gtcggtaaaa
    16621 ctgcgatggc taaagctgta gcaagagggc tatacggtga tgaaaataaa gtccttttag
    16681 taaatatgcc agactataaa actaaagatg atgctatgcg gtttagaaag cttatcggaa
    16741 cacatgtccg gcatgatcca tcagcaatca ttgtttgtga tgaatatgaa aaagcaggcg
    16801 tagaaacact tgatgccttt ttatctattc tagatgaagg aagaatcagt tttactgaaa
    16861 gaggatggga tgatcgagaa gttaatgata ccgtctcact ttcaaatacg attttatttg
    16921 cgacaacgaa cgcaggggct gagatgtacg attcgatgaa ccgatacagc tcactatctc
    16981 gtgttgctaa tcaggaaaat tcaaatgagt acagcactga gcttaaaaga gagtatcgaa
    17041 aggtagcatt agagattaaa caagctctaa ttggtgaagg tctaaaaccc gaagtcatca
    17101 accgatttgg acggattgtt ccattttggg ctttatcgga acaaactctt ttggaaattg
    17161 cacacaatga aatattgaaa agtgctgaat tggtaagaaa acacttaaac gtagacttac
    17221 gccttccacc acagaaaaaa catttctatg gtcaagaatt ttatgcagat gatctctcca
    17281 tgcacttagt ttttgataga gcttcacaag aagattctaa atcagggggt gcaagaaata
    17341 tcttgcatct ggtcgaatca gaatttgaag aagcaattac tgaagcaata attacctatc
    17401 ctaacgccaa aatatttgac gttaaattga gcaaagcagg agggtttgaa gggaaaggcg
    17461 atacggatgc agaaattatc gctaaacctt ccatcgctga aagtaaagaa aacaagtttg
    17521 tgttttcggt tggaggggtg tagctatgaa atttaaaaag tctttacttc aaaatcgtaa
    17581 aattaagcga tgggttgtaa gaggtttagt tatcgtgggg gcacttgcag ttactttagg
    17641 cgctccattt ggaatgaatg atccaacgtc tgatgatcaa ggaaataaac aagaaacagt
    17701 tgcaaatacg aatgtggctt atgctgaaag taaaccggca gaaaaagggg tatggacact
    17761 atatgctcgt atcctgatgg gagataaaga ggtcaatgat agtgccacta aaactggtca
    17821 aatgaattta cttggtcaag gaggtattag tgctgacttt ccttattctg atatttccaa
    17881 acaaggtaat gcaattgccg gtggtaacgc aggtacgcaa ttagcatcaa ccttagcgac
    17941 ttataattac tatgggtata tcgaaacagt ttcaggaaat gcgattgcaa gtcaggcatc
    18001 ctcttttatt ggtggtatcg gtagaacgct tggcagtttt atcggatcaa ttagcttgat
    18061 actttctaat attgctgaat ccatcaatga actccttgga aaatttttca tgagtatgaa
    18121 tatattttca cttatgggtt ggggtaaagg tggatcggat gtttctaatc cggtagctaa
    18181 ggcgttaact agcttactta ataatttggg attaaacaat caattattta aagcgatagc
    18241 aagcttatct atgctctttt ttacagcaat actaggctat caaggaatga aactgttgtc
    18301 gaaagagggt gttaactcaa agggattatc accagtactt gaaaaatgga ttgttcgtat
    18361 ttttgtattg tttgtttttc taccagttgc aggatcgtta ttgggagttt atatcaatgg
    18421 tattcagtca gcaaaagaag atggaaaatt accaacaaat gttgttggtg attacatcat
    18481 taatacaaga ctttgggctt ctactcaaaa tcttgctcca tcatccgata ctggtgggaa
    18541 tgtgccaaac ggaaaaccaa catctgaaat taaatttgtt gacactgatt atgaaccatc
    18601 taaaaaatca gaaatgatca gaatgatcaa tactagcagt tacgctagac aaaataatga
    18661 tctagattcc aattcagtcg gcatggcatt gttaaaagaa tggtcaagta atgatacgtt
    18721 taatgtcaat acttatgccg gtgatgtgaa tgtagctgaa aatgatgctt atatgggaag
    18781 agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa atgcaattaa
    18841 gaaacctacc aatttaaatg gttatatttg gtcagctaat caaaacgtta cagacgacaa
    18901 tcgcaagcct gatggaaaag gaacaacgaa taactttgat gctactcaac cttggggtgt
    18961 tgctaacaat gcaacgttta gcacacagtc ggtagcgttg atgctccaat cagcactgga
    19021 tacatcaggg gctaagtttt atgcgtataa tatcgctcca tcaggaattc aaggtagtgc
    19081 taagaatatg tcaacagtaa agacagaatg gcgtacagct tctttagttg gtcatggagc
    19141 tatgggtaaa ttcggctcat ggttagcgat gaacgctgaa tcaatcgtat tagcagtgct
    19201 ttatctagct tgtgcttggg cgatgtttaa cgttaatatt gtaaaagcct tcaaacgtga
    19261 gtttgtgcga gtttgggaag tagtcttaac agggtctctt gccgcaggag caggggttat
    19321 ggcaatctca ttaggagtcg taaccacatc tcttatcgga ttacaattac ctatttttct
    19381 gatcaccttt gttcgtgcat ttggagatac ggctgtagga ttcatcccta acacattact
    19441 tggtgatggt gtagcaaata ttgtacgatc cgctatggtc ttaggcgtag gataccttct
    19501 agtctttaag aaaggtgaaa aggttaataa taatttgatt aatatgctta ttggattacc
    19561 acttgaagta gcgtttggtt ttgacgaacg tgctaagtta ttatttggca atagaaatga
    19621 tgctgttcga gttgtggatg gcgtttctaa agcaatcaaa aaaagccaag gggctaaaaa
    19681 tccttattat gatggtggca gaggatcagt aaaagatatg gtcgcaggag gacttggagc
    19741 agtcggagcg caaggaattg ctgatcgttt gaacaatatg ggagacagca atgctgatgc
    19801 taagacaatc gctgataaag ttgttcctga aacgcctgct tcagtaccat ttaatggtgg
    19861 cggagttagt ggaacaatca ataatacctc tttcaaagat ttagttcgtc aagatcaatt
    19921 acgtaataac tcgcaacaat atagcgatca tctaggacgg atggttgatg aaaaaggaaa
    19981 ctttgtcgat gaaaatggtg aagtattaaa agacagtaaa tttggtaaag atgccaaagg
    20041 aaactttatt gatgacaaag ggcgtttggt cgatgaagat ggaaatttga tagatacaaa
    20101 cggtaaacca ttggaagaca atgaagatgg aaacatcggt atggatagcc aaggacgttt
    20161 aattgatgac aaagggcgct tggtcgatga cgaaggaaac cttgttgatg aaaacggacg
    20221 taaattactt aacggtgatt ttgggcagga tgtcaatggt cgtctagttg atgataaagg
    20281 acgattagtt gatgaagacg gaaatctggt cgatgagttt ggtaagaaac ttggaaaaca
    20341 cagtaacggt tataacgtaa atggcgatac agtcgatgaa gatggaaaca tcattgattc
    20401 aagagatgct cttgtagcag atcgaaataa agaactcgaa caacaacagc atacaccaat
    20461 tgcggttgca atccctgatg atgaaagcga tgaagttatt ttagatcaag cacatcaagg
    20521 gttaagagaa gaagcaaagg ataaagttgt ttcagatcaa gcacaagaag agctaaaaaa
    20581 taaatcgaat ggtgaagatg tttcaaacca atatcatcaa acggctggaa atgtacctgt
    20641 tgaaggtcag tctaatgagc ttcacaacaa ctttgataat gttgctgatc ggaatgatac
    20701 agtcttaaaa gaaaaagaat cattaagaca agaaacagat aaagaccaat caaaattgaa
    20761 gaaaacaact attcctactc ctattggttc aacagttccg aaggtcaatc aagctaacga
    20821 ttctgaaaca aacactgggt taggaatggc gaaagacaat caatccatta gagaacctaa
    20881 aaagacgact gaaagtagca agtcgaacaa gtctaaatct acttcaaata agacagggaa
    20941 atctaaggaa ccatctaaag ctaaatcagc agtagaatat ctatctaaaa ctggtaagaa
    21001 cgtgtatact tcagcagata aagctttagc aaaagcgcca aaaggaagct tagcaggaat
    21061 gacctatgaa gctaagaacg aagctatcaa attaggtaaa ggaactgcta aagttgccaa
    21121 agtaactgct aaaggagcaa ctaaaactgg taaagcagtg aagaaatcta cgatgtcggt
    21181 gaaagattat attgcaaaat atagcgaaca aaaaccaacc gataaaaagt cttaagaaga
    21241 ataaaaagag ggagcattaa tatgctcctt cttttttgtt cttctcttca aggggaatgt
    21301 caagaaaaac ttaattgtct tcaaaaattt cttttttatt tggtagtaaa tctatgaaat
    21361 atttgctttt ttagttatcg gagaataaga gggcttatgc tagttagtta aaatcaggta
    21421 actagtcatt aaatgctgat aaatcagcaa tttttacgaa aaaaaaaaat ttgagggaaa
    21481 atacctttca atctacaaaa atttatgtta ttatgataac ataaagatgt aaatatcgaa
    21541 gcatcttttc tgtattccga tcattgacaa gtgaacagag aaaagtttgc gatgatataa
    21601 ttatcttgtg aaaaataatc gaaaggaagg atttataata tgtttgattc tattaagaat
    21661 ttggtttccg gaatgacgcc agaaatgaaa acgttggttc aagctattct atatttaatg
    21721 gctacatttt ttgcattgaa accaggattc actgctatga aacatttcgg tgatgcaaaa
    21781 tggggagaag gcttaaaata tgttggagct gttgttgcta tttttattat ccctgtaatt
    21841 acaactgtta tgttgttaac gatgggtact aaaacaggta atgacttaaa ccaaaaagct
    21901 aatatgatct cagctcttat tcctgtggtt ggtatttatc tagttacaaa gtttccaaaa
    21961 gaagaacaat taggttaaaa ggtaaattga gcaaatttgg atgatatgcc gatttgttca
    22021 atttactttt tgctaaaaaa ctacactcga agcgtagttt ttgaagtgag ggaagacaat
    22081 agatgtaaga tggacagaga aaagatgttg gttcacgcaa acgaagtatt gacgagcggt
    22141 tttaaaggta ccgatttggc gaagataatg aatatgaacg taaaccagtt ctatgattat
    22201 cgaaacggca ataaaaagat tgaaaaagcc agacttgaaa cgctcatcaa atttgaaaaa
    22261 gcgtatctat acgtgttaga caaacagaaa agaactattg accgaaaaaa aggagtttta
    22321 caataatggc aaaattcaaa gatattaccg aatctattaa ttatgtagca gaaacagttc
    22381 ccgtagttgt actctctttg aagccacaat tactcgaagt gtaccaaaaa gaagaaaaag
    22441 aagcgaaggc aaaaaaagct aaagcaaagg tatggaaaga attcgatgcg ttgctgacca
    22501 aagatttacc aaagaagaat tacgtggtga ttgaggacgt acaaaagctc agcagtgagg
    22561 aatttccgga tgaagaagat gcgattctag attccgaaaa gtacgttgtt ttattaaaat
    22621 caagtctgaa ttatgttgtt gatttaccgg aaaaattcaa agaaaagcta ttagctggtg
    22681 tccgctttat ggatagtaat ggtcaattat tagttattcc tgatgatcct aatatgtttg
    22741 aaagagaagt tgaagaagct acagacttct acagtgcagt agatcaatta gaggtagcta
    22801 ctaaaggctt agaaagaatt gctgagacct tcaactcttc tgatgcggtt gaatttgaag
    22861 ctgtagcaga agccgaaaca gaagccgtac cagatgaaga agaagctctt tttgaggata
    22921 atgacgaagc aactgaagca ggaaaccaag aagcaagcat agaagaaaca caaacagaaa
    22981 cctaccaaca accaaaaatt gatgaaaaac ccgaatccca gagtgataaa gaaaattatg
    23041 tttatgaagc tgaaaaaatc gtatctgaaa gtgatgtacc gaaaaattta gatgctgatt
    23101 tgttagaaaa agattttgaa cagatcatag ccaataaatt tcaacctgta gttttagatg
    23161 aggttagtga tcctgttgat ttgaacgaag agatgcgatc cattgcagag ttaaaaccat
    23221 tgctgttttt agctaaagag agcatgaacg ctcgtatctc tattgctaat aataaaatca
    23281 acagtgaatt aaataaaacg aaagagcaat tgctactcaa aggaaagcaa atgttagcgg
    23341 atgagctttt gcggatagag gaagcaacat cactaagtgg tcaaaatcag tttacaaata
    23401 cgctatcggt cgctaaagaa agctacgaag cagaagcatc taaaattgat gatttagtga
    23461 tggaaaaatc cagtatcatt cgtaaagatt atgatatggc gaaacaggct tatttggaag
    23521 cagaactaaa gaaactagag caacggtacg atgctgaaca tctgccagaa gttaaacaac
    23581 aagaagatga atttagaaaa gcaactgaag aaagtcttga tcaacaatac caagaagcaa
    23641 ttgaatctat taaaaacgat gctttgaaac aaaatcaaga agcaaaagaa gttgtggtgg
    23701 aacggatcat caattcattg aaaccggaaa ttgaaaaatc tttaacagaa gtgcaagaaa
    23761 aaacgttaga gattgtcgaa aaaacaaagg cagaaaatga agctgagttc cacgatacaa
    23821 aagagaaaac aacagatgtg gttgtcaaat attttgcgaa caaagaagaa gttgaaaagc
    23881 tagtagctaa agatacgaat gagttagaat ccaaacttgt tgcacagcaa aacattattg
    23941 aagattataa aaatcgagta aaagagttca aggatttagc agatggctat aaattaaata
    24001 gccaaaaatt gactgaacaa agtgaagctt tacgagaaga tttatctcgt gcaagaaacg
    24061 acctatcaca aatggcggaa aaaacaaagt cactagtagt tactacagaa aatcaaactg
    24121 agtctaaacg tgattttaca agcaaaaaga tggcgactat catcgctgta tcagcaatgg
    24181 cttgtggaac gattttgggc tcatcctact tcattagtca taatcaccaa gaagcccaag
    24241 ctcaaacaga aacctcaatt tcaagagtgg aaaattcact aggatcaaat acagcttcta
    24301 cttcaacaac agacaacaac tatgaagcaa caccaacgac agaatttaaa gtcggagaag
    24361 atgttgtcat cagtattgat gggaaaacta ctaaggctga agtactttcc attgacggtg
    24421 ataaggcagt tgtgaaagct ccagatggga atcaatacaa aatacaagct cacttaactg
    24481 aaaagaatta aggagggcta aaatgcgaga agaaaatatt gtttatccag taattgaaaa
    24541 tcaagcaaat acaaaatctt ctcaatttct tcaagaagtg caagtcgttt atccggttaa
    24601 tgaactacca caaacgaatg aaacagtgag caagtcaata tcattaattg gcttgcttat
    24661 tgttgcaaca gcgctaactg ttggtgttta tcgtaagctg aaagtgaaag gaagtatttc
    24721 tgatggcaga taactcaaac tatggattta aacataattt gcatcaaaaa ccttctgaaa
    24781 caatagtcag agttgattgg cttgaaaaac atgtcaacag cacctattct gttacagcaa
    24841 aaagactaat tgtttcagct attgttgttg cagttgttgt tcttggttca atcgtttatt
    24901 tggtttccga tacgattaaa ttgagattta tttttattcc aacgctattg atgataattg
    24961 gttttcttat actgattagt cagtatatgt ttattgatcc atctagcgga tcactgatct
    25021 caggctctat tagactgttt aaatcagtag ttaacacagc aagagtaacg atggggaaaa
    25081 agaaaccatt tcgaggaacg gagattttga aagttagaca agatggaacg attatgtatc
    25141 gagatggtag tgtagcacta atgtttaatt tggatggtgc tacctcgtta accgccttcc
    25201 cagaagaaat catagcgcaa gaaggcaaat caattcaata ccaaaatgca agggatcgtt
    25261 ccttggctga aatcaaaatt acttcatcac aacctcagaa tacggaacgt caaatacgga
    25321 acatgaaagt acaaaaaaga aatgcaaaga gcaacaaagc tgtgtttgac atagtagatc
    25381 aacaagagag gtttgtatct gaatatattg atggggtcaa aactacggtg atccaatatc
    25441 ttattttgaa gtcacaatct cagatggtat tggatgaagg cgtagaacgt ttagaaaatt
    25501 acacgagtag agggctttac tatagcgtta ctcagttgtc agctaaacaa accaaagaaa
    25561 tattatcaga aatttatgca ctgaaataat agaggtgaga aatttaatgg aactaatgag
    25621 tagatcgaag ctacttaatt tcagaaaaaa gaaacacaaa aaagagcaaa atgctgatag
    25681 ttctaaagcg gtaaataata cttatgtcag tgatttaaca tcattggttg gttatcaatt
    25741 tacaccatct tacattagga caggtaatcg gtggggaacg attgttaaat tagtgaatac
    25801 gtatggaatg aacagaaatt atgaatttgg atggatggtt aaagctattc ctgaagtaaa
    25861 ggttgaagga gtaaaagctt acttaatcga agcggataag ccagtttcgc aaaaagatca
    25921 gcaagaaatc atgcacgaaa atgtcggtgg atcaacgaac agttatgaaa ataacttgaa
    25981 cgataaaaat tcagatgcag aaatcaataa aactgttatg cgaatcattg atttgaatac
    26041 agcatctctg cttgacagta cggcaaacaa aattattgat tggaagttag aaatcctctt
    26101 agtatccgat tctcctaaca agatcagcga acagttgatc aaactgcaac gcctgtatga
    26161 tgaaaaaatg tatggattaa aacttatgtc ttttggtggc gatcaacgtg atctgtttga
    26221 taggctttta agtgagccag tcggtagtgt ttacgaatac acatctatga gttctgattt
    26281 tgcaggaaat gatcatgcta ttcgtaaagg cttagatgat gcagatggat gggcgatcgg
    26341 tacgatgtct acaagctacg cttcaggtgt agcgtttatg gcaatggatc ggtcttttaa
    26401 gggtaaagtc ttagtagcat cccacaaaga tgcaacaatc aaaggctata gggaagaaat
    26461 ctcagcatca tcactttggg ggcaattgat tgctaataac gcaatggcaa atcataaacg
    26521 gacgtttcac atcgtattaa ataaatttga ctactttggt gatccgacag gggaaacatt
    26581 tgttgctaag ccatctttaa atagtgtgat agagaagatt aatctagctg aagaaggact
    26641 taattcttta gagctttttg gtgatcgaaa ggatattgga gatttatata gacgtaatct
    26701 tgataaacta actcagattt tctatctgtt agcggacaga gaattaaatc ctgcaacaag
    26761 agtgttgctg aagaaagagt tggggaactt ttatattagt cagaagcttt gggatattaa
    26821 ctatgaagag cggtcgaaac tgttagtaaa cagcagtgat acaatgccgg ttcttggaga
    26881 ttttatcagc tatttaacaa atgctgagac aaaagcgaaa aaaaccggta cagaaaaaga
    26941 aattgatcgg attattgtat taaaagatac attgcgaaca gcattggata gcttctctaa
    27001 gatgttcaat cagagaacta cattgaaaga aattggcaag agcgacaaat tgcagtttta
    27061 ctacaatttg agcgatttaa gaacagaaat caatgtcatg gaagcccaat ttttaaatac
    27121 ctttgacttc atcacgtcac aagctaaaaa aggcgatatc gttatgatcc atgggtttga
    27181 tagattatca gttgaaaccg ttggattaat taaaaagcgc ctaacaatgc ttgaagaaga
    27241 tggagttcgg cttgcgtttc tctttgatac tatcggatca ggagaagata aagacggtat
    27301 cgaaacagct gatattttca atacagatgg aaaaatttat caaagttttg aaagtgattt
    27361 taactacact atcatcggga aaatgaccag tgaagaattg atgaagtatg aaaagaaggt
    27421 tgctcctgca agaaaaggag aagatgatca aaaactatca gtggatttga gacagtattt
    27481 gacatcaggt cgaagcaata aatttcaaat cagaagacca gtcgatttga cgaacaattt
    27541 tgttattgga gagtttatta tttaagttct atccttatgg tatcggaaaa ttgtgtctag
    27601 aaggaagaaa tatccgtcaa aacaaaccat actttttttg gaaaagcgga gttttagaac
    27661 aatttcctag gagaaataga taaaacaaaa ggggcgaatg gttatgaaac aatatttaaa
    27721 tgaatggaaa gtgatagagg ggtcattagt agcacaaaga ataaagggat tacctgattg
    27781 cttagagaag gatcatttgt ttcaaatacg agaaatgtta aaaaaagaac agtttgatcc
    27841 agaccaattt ttagtagtcg agtatccaac tattggggtg tactgttgta accatatcaa
    27901 tgatgaaaaa tattttatta ttcaggaata tgaaggacaa ttaactcctt tctatactac
    27961 atgggaaatg agtgaggatg ggatcaataa ttttccatgt gaaagcattg aagagtcaat
    28021 atctcaaacc gaatgctaat tgattataac tataagaaag gtagtgagtt aatggataca
    28081 gctatattgt atattaacat aattccgtct atatccttta tcattactct tacaattttc
    28141 atacgtagaa aaaaattttt tacagagaag aagcttgtat gctttttgat gaaaagtgga
    28201 gtagtatttc ttttttcttg gttattgctt attttatcaa cttcaagttt tttaattata
    28261 ttatttgcga ctgtgggtct tatagacttt ggtgtaatat caggagcagt aatgtggttt
    28321 tggttaacta gaaaacctaa aaaaaggaac aagaaaaatg gtttggaaaa taaacgatta
    28381 tttagattaa tgacaaatca ttaaagctat aggcatatta agagataata tttaccaatt
    28441 tcctgattgt tttgaaaacg agcatctgta tcaaatcaaa aacatgatga attgattttg
    28501 ttatattgaa atagatatta agaaaaagca ttccttgtta aaacacaagg gatgcttttt
    28561 gtcttcactt aatctgttta atataatcta acaacttttc ttcggaatta attttttcac
    28621 ctaggtattg ttcagactta ttaacaagat tcaatttaag atcatttaat atttctggat
    28681 acgtatcact tttcgattca gctaattcat acagtttttt tagttcctta tcttttaact
    28741 gaaacttctg atccatagtg acagcatttg gatatagaag tccattataa tgtgtgcgta
    28801 aatccatgtg gaatttttgt ttataaaagt ctagcagatt agaaataaaa gaagaaaaat
    28861 aaccattata gtaaaactga ttatttctat ttaagttata caacaattta ttaaattgat
    28921 cctgatttaa tttgtcgtaa atccatggca atatttccat gtccttatct gagtatctaa
    28981 atgcaggaga atcggtcaga tgctcaatca taaaatctaa gaacatctct ttttcaccaa
    29041 aatatagata ttgattatat atcgtttcta aggtagaagt tgctagagaa gagactctca
    29101 tgttttgctg aagtattgga tcaagtagtc tatttaaatg ttcttgtaat tcttccttgg
    29161 tgtttcctag aactgcaagt gaacgtagac ggtagtaatc ttctatatgt gttgttattg
    29221 gctgttttgg ttcaaaatcc tttattgata tttttaagtt ttgaattatt tcttctgaaa
    29281 aatcacgctg taattctgga tgactagcta taaaggtaag caaaaagaag ccttttgtat
    29341 aagtaatctg ttgattttct ccaagtgatt tattaatttc ctctctcgat tcagctgtga
    29401 acctgtagtt tgagatttct ttttttagaa tttttaaata aaattctttg ttatcaaaaa
    29461 tcagggcatc tagtagtttg aaagtactta ctctatctct atctacttct tcattcccat
    29521 aaggtaattt aaaaacaaac ttaaaggctg ttttacaaag gttttctttg gctttctcac
    29581 ttagtttttt cagatagcta ttttttagtg cagagtggaa ttcttcaggt gtaacagaca
    29641 tagaatgtat tcgacttgca tattgtatga tttcgttcgt tacttttttt cctaatattg
    29701 gattttttag aaatacaatt tcataggatt tccgtatcac atcctgacaa gtttcctttg
    29761 ttatttctct taattcccaa gtgtcaccat tgtaagtgac tactggatga gctgaataat
    29821 ttctttgatc cttaatatat tttaaatcct catattcgta gctatcaatt agctcagtcg
    29881 aattataagt tttttctagt agctccattt cccatcttgg ggagtttgga tcactttttc
    29941 ttaatttagc tatttcttct aaaattttct tagaagtatc atcctcgtat atatttgcta
    30001 aatcagataa tttatcaatt aagtctaaaa caagcgcact gtaattgaca gctatcgctg
    30061 aacgataatt tccctgataa aaactacttc tgacttcttc taataaagga acaattctag
    30121 gatttttaac tttgtttatt tcatcatcta atataaacat ataactctcc ttttttgttg
    30181 atatgacaag tttaccacaa aaaataaatt aaaaaacgag acatatttat gttattatgg
    30241 tatcataata acataaatat atggaatgag atgatgcaaa atgggcttga aacaatcagt
    30301 ttacatctgg ttatatcctg ataatcccat gtcaataatg gctgatattt ttgtaggtag
    30361 cattttattg tgtttactgt atctaatttt cttccacaat ttgatcgggt acatgatcct
    30421 tgattggaaa ggaatgttcg atatagaaag aaaaccattt aataataagc aaatttatac
    30481 cacctttgca ggtggagatt tggaagaaaa agaagttgat gtgacacatg aaattggatt
    30541 agctttatca ggaattcaag atgctgatgt attaggatca atgataacgg ataccggata
    30601 taacgcctat gaagtatttt atctcatgta ttttatatgg cgagatagtc aagatattat
    30661 caacggtcgt gctgaaaaaa caatcatttc aaatactgaa atttctttct gtatgggtga
    30721 aggggaaatg attgtatata aagatggatc agcaaacatt aataccgcct ttggtgatat
    30781 tgaaactagt attaaagagc ttagggagta tgtacagaat ccctacaatt ttcaatattt
    30841 agcaggaaga ataaatttag tttaggagta agaatatgaa aaaaattatt gatcagccaa
    30901 caaatcgctt aatagtcgaa ggcgttattt tcgtaacaat gtatttcact gtgggaaatg
    30961 gtatttcaaa tacaatcatt caaaaagacc cacaatactt gttgtttagt ctttatgctt
    31021 ggattgtgca gatgctttgg gtgtctttat taatgctgtt gttttattgg atagtacggg
    31081 ttattctttt agtgttaaac aaagcaaaaa agaaaaagga taggtgagta aaaatgaaag
    31141 caacaaaatt aaaacttgta ctattttcaa cagcagtatt actaacttta tcagcatgtg
    31201 gtacatcaaa gagcgctgat aataccaaag acacaaaatc tagcagtaca gtggtgactg
    31261 aaagcagttc agcgcagaca tctacaacta tgtcttcaat gactgagaac aatgctgaaa
    31321 aatttcaaaa gtataaggac gtaaatcaag gtaaaaagcc ggaagaagtt tataacgagt
    31381 ttatctcttc agtaaaaaat gatgaaagtt atgggaatga tccattacaa aataaaaatc
    31441 tgactattgt tctttacaaa cctgattgtc ctgattgcca aaaagcagaa tccaaaatta
    31501 tgcaagaaat acaagaagga tttagttatg ctccaacatt aatgcacgat attaccatcg
    31561 gcaaacctgc ttacgaatat aatgcagttg cggtagatat aacagatggc gtacctgaat
    31621 gggtgcaaaa tatccctggt ctggctgatg agaacaacca atatcacacg ccaacattag
    31681 cagtagtaac accaaagaaa attgatggaa cactatactg ggatttaatg ggaatgacaa
    31741 caaatacatc cgatgaaatg attgaaagca ccagtaacta ctatatggat ttgggagcct
    31801 ataaatcata cgaacagtct gaacctgtta tggatgatca atctactatt aagtagtttt
    31861 tagggcgtaa gccctatttt tttaaaatta tgcgataaca aattaatgat ttctgaaaca
    31921 aacgttctta ctcaactact cgaaacacac aagctaatta actagattat aaaggagaat
    31981 gcgtaatgaa agaaatcagt gcaagtgaag cccatatttc tcaaaagaat caggttggta
    32041 tatataaggt gcttcatggt gaagggattt ataaaataca gttacttact ggttacaata
    32101 acggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga agtgaagtag
    32161 aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga aataattctt
    32221 ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa attgaagaat
    32281 actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca gaagaaaaat
    32341 ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag cccatgtata
    32401 actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa ttaaaaggca
    32461 ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg aaattgattc
    32521 aagagttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa gaaaatggga
    32581 aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt ggtttatttt
    32641 catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt ttggacagtc
    32701 cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag atgactatat
    32761 ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag tcgaattatt
    32821 tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa ctttagagga
    32881 atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg agcaggcaaa
    32941 gcaaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat gcttggataa
    33001 gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata gaaatgcact
    33061 aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa agcaaatgtc
    33121 acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca cagttcagag
    33181 attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt caagcaggaa
    33241 cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag aatatggtgt
    33301 tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa cacctaacga
    33361 agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag agctatcgca
    33421 aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta ttgtcggttt
    33481 tgatattgaa agaaaatcca aaatagattt tattactcaa ttaaaaaaga taaataattt
    33541 agatattcta acgcttagac cagatcatga acagttgttt atgagtttta aaaaacaatt
    33601 gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag ctcaaagaaa
    33661 tcagagtttt ttggataatt aacaaagaat ttatcatctt atatactctt ggatttctca
    33721 gcatgatctt gttttggtgg ttatatttaa aaaagtaatt ggagagggtc aagaggattt
    33781 ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac aggattgtta
    33841 ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga acaatcctat
    33901 tttgaagttt ctgctaacaa cgtaatagaa aaatgctatt acggtcaaca ttattggagt
    33961 gaggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg ggatgcatat
    34021 tctatcaaag attatcctaa aagtttaacg agtagattat tttacagcga aaaagataat
    34081 caaaaactct cagatttgat gatgaaaaaa gtaaggaaac ttgcacaaag ctatttggaa
    34141 gaaaaagcag gagtaataaa agataaggaa taaatggaga aatttgctat tgaatgaaga
    34201 aacggaatta tttttaaaaa aagtgaactt tcttgtagaa aaatattttt taacaaagca
    34261 caaaattatg tatctaataa aaggattgat cggtggaatt attttatgga tacttacttt
    34321 cttttcttta tttggtggat ttactaatta taaaaagtta gtagacgaaa atgtgaagca
    34381 aattagtgca tctgaatcat taagacatca agctgaagat ttagtaagta atccattatt
    34441 tataggaggt atggtagaag tgataataat tagcttcgtt attggttatt taggtatgtg
    34501 tatcttccag ataatctttg atctaataag aggaaaagtc tttcaagaat ttaaatctga
    34561 aaaaataaat ttacgttaga gataataggc tatgagagga gaagaatagt ggaacaaact
    34621 aatcatatta ctgaagaaac taggaaattc atttgtttag aatcgtttta ttcggaaggt
    34681 aggtattgta acaaaggtga aacttatacg gcatatccta ttgaaggggg atttaaatta
    34741 gtttttgaaa acggagacat gaattttacc actgagttgt ttgagtgtgt tcttgaaacg
    34801 tggtcagatg tgttgcttga agtcactaaa taatcaatgt tatctagtag aattaagcaa
    34861 ctaaaaaaga aagaaggaag aaaatatggc gtatgaaaat ttacaattag tagtagcagg
    34921 agttagtggc gatatatatc ttgcaaaagc aaaagatgga attatggaca cgaactatag
    34981 aagagttgct acaaatgacg ttttagtagc aacgacagaa tggttcatta gaaataacaa
    35041 aaagttaatc caatttgaag cagatgaaaa tggtagacat agcttgtttt atacaaacga
    35101 tcaaaaaaaa gctgatcgga tcatagcgat tctaaaggag aatgatgatg gtggacaaag
    35161 agattaaggg cgaaataaga ttatttcaaa gagaaaatga cgattggggt acggaaatat
    35221 caaagaatct tgctgaagat atagagttac gattgagctt tataagtatg ataattgaaa
    35281 gttttgcttt tgagcttggt ataccgcaag aaaagttagc agaaatactg attaaagcaa
    35341 gtaacaatag tcctcagcac tttcacattg atctaggtac agtaaattag tctttaatca
    35401 ttaatattaa tttttttgat aagctgatct gattaatatt tcttaatgta atcattccaa
    35461 tattgatctt cagccgttaa cggaatatat agaggaaagc ggtgaaatga aatgtctgaa
    35521 aatcatttga aacgattatc gaaaggaacg gcaagtatgt ttgatgccaa tctactaatc
    35581 tttaaaagcc tgctagaatt tgaaaaacaa cgtgtagaag atattgctaa gtatggtaac
    35641 tatgctactg ttccgagtgt ttacaatgat ttaatgaaat ggttcaaggt acaaacaggg
    35701 ttggaatata gccaacagaa tttagtggta aaaatagagg agtatgaaaa tcatggctaa
    35761 aagtttatca caagaattaa gagaatttgc ttttagagag tgatgatcct atagctgaac
    35821 tagatcaatt agcggaaaga gcaaaagaat tagaaatgat ttctggtgcg tatagaatta
    35881 tttctgaaga attaggtcaa gcggttgtgt cacaagcact tgaaaattat ttgaaaaaac
    35941 aagttgatta gtgacagaaa taacggaata ggatataaat gatggattca agatgatatt
    36001 agttaataaa aaatagtatg gagggcaact aattatgcca gtaaaaacaa acactgaatg
    36061 gttagatata tgtgatgaac tatttgatac taaagacaag aaagaactgg cgatgcgtgt
    36121 agcatatgct atagttcaaa aagataaata ttttgctgtt ccatctacag actatgacat
    36181 tgaagtgata gttgacccta ttactggaga taagaaatta cgagtagtta ttgagtgtaa
    36241 cgcctaatgt attctttaga acaataggag gaatcttatt tcttaaaagt gcaggaatca
    36301 tatagaagag tggttgctaa ccgtcttaag tatattagaa agagatgcaa atttaatgga
    36361 tatttttgaa gttaaacctg attcaggaaa atttattgtt ttgcaggaaa aaagagtgat
    36421 tgcaattgaa agaacagaaa tagaagcata taaaaagata catgaactag cagaacaata
    36481 tattgaagat ttgaacaata attttcaaga atttctaacg caaaatgaat aggcaggtat
    36541 agacagagat aacaaactag gaaaggaaga ccataaatga aaatttttga aattgatgac
    36601 ccgtattttg cacttatagt agctgaaaac gaagaaaaat gtatggatat atatgaagaa
    36661 gtaatttgtg atgtagaagg caaagaagaa tttttacctt gtataaaaga acttgaaacg
    36721 acagttgcga ttacaaaggt tgcaaatgca gtgagtaatg aaacaaacga gctagtagga
    36781 attcatgaag ctggtaatct tgtgtttagt tgtcttaata gcaataaatc tgcattattg
    36841 gctcttgatg gggatttatt ttaatccaac aaccaccaaa atcacctaaa atggtcgatt
    36901 tgaccagttt ggataaataa ttgtgtagat caattttgta aatgaagatg ttagataaac
    36961 tagacatcaa tctgtatagc taaagaaaag agaacggtaa attatatccg ctctcttttc
    37021 tttagctatt tatagaatat accttctcta aatccttcca tcaatgtgag cttgcttaat
    37081 tcatttgaaa ttaaaaaagt tattgctatt atacacatcg ttgtgtataa tataaagtgt
    37141 aaatataatt gttcctttga gctattcatt agagttgaag catagaatag tttcatagaa
    37201 tccatagtaa aaaatttgag agtaaaggag aagttatgag cgaatttgaa tttttagata
    37261 aaagtattgg ttctgctttt ttgattgatc ctgatctgtt tggagcatgg gctcaatcat
    37321 caggttataa ttgggctttt agtgatgata tggtcgagct tttaagtcaa acgatgaatt
    37381 gggaagctta taagatcaag caatttttag atgttgttgc acagccgatt tatacatctg
    37441 ctgatgatca ttggtcaggt agagagttga caacactcaa agaactagcg gagtacaata
    37501 aaagatacaa aattccgttt caagtttctt acatgtcaga tgatttttcc gttatttact
    37561 caataggtaa agaaagcaga tatttaatcc ctaataggca cttagagata aagatgaata
    37621 gcgattatac cgactatacg ctaagtgagt taaaagctct tagacagagc gacagtgagg
    37681 aatttagcgc actcaaaaat gaagtgagtg cagataattt tcgcagatca gatattgaag
    37741 aactgaaaaa aaagcaaaaa cagcaactag aaaagcaaaa aaaattaatt gaagacattg
    37801 aatataaacg agtagaagag cttgcaatac aagaagccaa aattgaaaaa atgcgacaag
    37861 aactctataa aaaacagcag tcaatgttag cagaagcaaa taaaatgcgg tttgaattag
    37921 agaaaaaagt agaagatttg aatactaaaa ttttttattt ggaaactgag attaatgcaa
    37981 tcgaaggcta ttttggtgag agtacaactt ttgttcagtt gcgttcaggt caaaagagct
    38041 tgccggaaga accagtggtg atgtttcaaa aagtacgata tctagatgaa gaattaggaa
    38101 gatatttcag tatgcgagaa gttgaaggta cagctgatac catgctgact tttgagcagg
    38161 cgctatcctc aagagatgat ttgtttgatt tattctcacc gaacaaaaag tcaatctcaa
    38221 tgatacgtat ttccaaatca ggatatggct acactaacgg tcaaatggaa agtacaaaca
    38281 tgctagaaaa gtataaaaaa tatcatggag aaactttagc aattttactg agaaacggtg
    38341 ataacttatg gatttgttgg ttggatgaaa ataagatcag tttcaaagat gatttgtttt
    38401 accaaaacaa agtaacagag caggcatctg aaaccaaaaa tatggtaaag gagatagatt
    38461 ggacacatac tcgtaattta gaagagcgat tgaaagatgc taaaaagcag aagaaggctg
    38521 ttagaggaga gtttgctagt cgttatattc ttttttcaat tttgcaaggt gttctagata
    38581 atggaacgat gatgacctta ccagaaaaag taaatgtgat ccaagcaatt aatcagaaaa
    38641 gcccatattt tattgcaagc gtagcggatg gatggttaga agacacacgc ttgccgtcat
    38701 ttagcgagtt gattgataaa tacagtttct ataaagaaac aggtgaagac agagtattag
    38761 aagcggaata tgagttgaat cccgataaat ttgaaaatat gagagattat ccacacaaac
    38821 aaaatgatga catttatgtg ttataccggt tacgtgggtg gtcgcctaca tcagcaaatt
    38881 acacttatta caacagtgat aaccgttcaa atagtgagag tggacgtgac ttagcatatt
    38941 cgattgactt gaaagacggc atttatcaga tcaatttagt aaaacaggga aaattccaaa
    39001 aacaaatgtt ttcccgaacg tttgttggag ttgattctac tacaggcgaa aatgtctatg
    39061 attactatta tgaaagagaa acagagtact atattcgagg acaaaaagcc tacaacaaaa
    39121 gtgactattg gggtgacaag gcgaagcgat taactgcaaa ttttagagtg tatgaatccg
    39181 aatttataaa cttaaccttt cttaatagtg tttttattga ttatgtcatt actacgaaac
    39241 gtattggaag ttattcaagt acctacgcat ccttagttcc aatgttaaac tcgatgagcc
    39301 gtttcttaaa agaaagagaa gagaaagagc tagagtatat caaagaaatt atgccagatt
    39361 ttgaaatgca cgaatggcaa attgctttat cagattggaa attgacgaat aaggttcgta
    39421 gaataacacc ttttcaagcc aagcgctttt tgaaagctat gaaatttgtt caatagaaaa
    39481 atccaccaga caagctgatg gagactaacc caattactaa cagttgtaat tggtgttagc
    39541 ttataaatta attatggtgt ttagtcaatc ccaaattgtg aaacggcacc aataattgga
    39601 aaggaaaaat gaaatgtctg aagatttacg agagctgaag ttacgaaaga agcagtatga
    39661 ccagtatttt tccggtttgt tgaataaaca tattgaagaa ggacttgatc atgattttgg
    39721 aaaagatgaa tattgtttgt attgcgagcg ttacaatgaa aaaatgtctg aatatctaca
    39781 gctgaagaaa gagatacaga aattagagaa aaaagtacat gcttggaagt ataaagattc
    39841 catgtccggt tatggtgaat ctcaaaataa gccaagaaaa aagaagcaag cgtatgttct
    39901 aaagatggta aatggggaag agttaattgt attagggtac agtaaagaag aggttcgaga
    39961 actcgttgaa acacctgtca ggaaaaaggt agaaacaata aatgagatag acgacagcca
    40021 atacaatagc atctatattg aaacaagtca tggtggatat aagaaattag caactttcct
    40081 tagaagttct aaagttggga taatttctaa aaaagacata agtgctgtta ccgatagttt
    40141 ttgtacttta tctgttccta aagcatattt tcaatacaac agtaatagat ttgatcgctt
    40201 ggagaaattc tgtagaccat atactgtatt gaaaactaaa aaattcaata aaactaaaag
    40261 agtttcaatc aagttaccga ctggtcaaat ctcgctaggc gaaaaagatt atctgataaa
    40321 agtaggaaaa gagttaataa tcaaagtgcc ggaacgctta tttaaagaaa attttgagat
    40381 acgtgaaacc ttggtatgga aagaagaata atagtttgga agacatagca gggtataagg
    40441 aggaattgaa atggcagtaa atagaaaatt gcaggtattg attacagacg actttggcgg
    40501 agcagagtgg tttgatgtgc agtctataga ttttgaagaa gaagtcgtag ttatagaaaa
    40561 tgtatacggc gaagaatact catattcgct tgaaggagag aatgttgagg atttacggtt
    40621 gattggtgag tagaaaatag cagaaatatc aaaatggaga tgaaatcagc gcgtatatga
    40681 ttaacaagaa aacaggaaaa cgtattttca gcagtaaaga cgaggtaatt cgggatttga
    40741 gtgaaaactt tgaaccaatt gaattatttt tccatcagtt aaatctagaa attgaaaaat
    40801 tactaaagga acctacagaa ggacgaatta ataaagtatt gaaattaata gcatgtaaag
    40861 atagtcatgc ttacatgctt tatccaaaat ggtatgcaga caaaaatata gctgaaataa
    40921 acgattatct agctagtata aaggaacgca atgcaattaa ttaacaatag acgaaaaatg
    40981 aaattaaagg agataggaaa atggcagaag ttaaatttgg atcaaaagaa tggtttgaag
    41041 agttaaactc aatgattcag tttccagaaa agttcacttt ttactacaac aaaagagaca
    41101 atatcattca agtagcttta cttgaaacta agggaagatt agaaaatact cgatggagta
    41161 tgataaaacc atcagcactc agatttcatc ctaaagactt gacagcggtt gtagcggtaa
    41221 aagaggttca agactcacaa gaatactatg aagtagtaaa aaatctgaag gatatctggt
    41281 gtaaataatt ctaaattgac cagttatttg gtcaaaaata gtatgtgatt atttcatcca
    41341 atatggcatc ataattggta tgaaagggga taaattattt gaaagaacta gaaggtatta
    41401 agaaaagaaa tcaggaaaga ttggaactgt taaatgaaaa tagaaatagg atgtttagtt
    41461 actatgaatc catgaggtgt ttaaataaat ctagaagtga aattatagaa tcatcaaagt
    41521 ataaacaatt gatggaagag agagtttctc tcattcaaga aatagaaaca gagtctattt
    41581 ttccagatca ttttcctaaa gtccgatttt ataattaaac attaacaaat gaaacgatgc
    41641 taaataggca tcgtttttta tttatttgtt aaatttattt atttacaaaa tgtgtatcag
    41701 tgtgtataat atatttgtag ataagttaag aaaggatgat ctgaaatgaa attagaaggt
    41761 agcttacaaa acagaatgat ggaaggcaga acaacaaata aggagattgt agttggtatg
    41821 ggagcaaccg agcttctata taccgacaga aatccttata cagtgattga agtaaaaagc
    41881 aaaaacagaa ttttggttca agcggatggg gcaatcaata aagctacttt tccagatcaa
    41941 gagtgggaat acagcagaaa tcctgaaggt caaatattgg aattaattaa gacaaaaaat
    42001 ggatggaaag tgcttaaaga agatacttat ttttatatag gtgatagaga aaaatactac
    42061 gatcctagtt tataaacaaa ttgaagatga gcgaggtttt atcctcgctc agaaaggaga
    42121 atgaaggatg ttcagtcaag aagaaatcat cagcaaatcg caaccggata aaaatggaac
    42181 tataaccatg aagaaaaaaa ttccttgcta tcgttgtagc ggatcaggtc agtacaaatt
    42241 tatgtctatg gggaaaatta cctatggcac atgctttaag tgcgatggtg acggatttct
    42301 tgttgtaact gaaaaatcat ttacgccaga atatagagct aagctaaatg ctttaaataa
    42361 aaaaagggct gatcgaaaaa taaggatcac aagagagaaa gaatctcaga aaaattttga
    42421 tcgttatggt tttgaaaagg gttatattta cgtggttata gaggaaaata gctatgcgat
    42481 caaagatgaa ctgcatcatc aaggagctac ttatgatcct ttattaggtt ggtttttcaa
    42541 agaaaaacaa gaacaataca aaactcaaaa agtttttgta gaagaatttt ccgtaatcaa
    42601 tgatgaagga attattgaat tgaagctaga agaatacagc gaaagacaac aggagatttt
    42661 aagggcgaag cgtagagaag agggtgagtt tattggtaca atcgctcaac gattggaatt
    42721 ggatttgaag cttaccaata ccgtcaccat agatacacaa tggggcaatg tttatctgca
    42781 tttatttgaa gatcaaaacc ataattcatt gatttggaag acaaataaat ttctttatta
    42841 tgatgaatgg gatgttggag aaataaggaa agttaaagcg acaataaaag cgcatgatta
    42901 ctacagagat accaagcaga cttgggttaa aaatgttaaa attcaaaaaa atgagtcaga
    42961 agggataaaa gtatgaaaag agaggaatac aaacaaagat taaacgaact gcttgaagag
    43021 gacgaaacat taactcatgg ttcgcctgat gaaattctat acatgattga taatatggtg
    43081 atctttggtg gatatgaact tggtaatagg tcagttgatc ataatatttt agaatttgat
    43141 gatgtatcat gggaagaaat tcttgattgg ggaattttgg cggtacctga aactaaaacc
    43201 tatattagtg acactatggt tccttttttt gaggaattgg attataaaag acttcccaaa
    43261 aatgaaaatc atatactggg aggaaactaa caatgttaag tgacttattt attttactga
    43321 tctatctagg tgtaagtgaa attattgcgt attttttgtt tgtcattgct aattatggta
    43381 tttccagtgc aatctttcaa aggtttagat taccagtaag tgaactaaaa aaaatgatga
    43441 gattatcagc atatctttta atgtttttga atgtggtcac gttaatcttt gtagtaatcg
    43501 gtgtgacttt caatttaatg gaatagaggg aaatctataa tggaaaaaag agaaaagaat
    43561 gaacatattt ttttgaatca atattgttga tggtttttgc gatcagcgct taccagaaat
    43621 ctaaaaaaag agtaatgatt gtttaatcat cactcttttt ttgtagggaa attgaaatat
    43681 gtggagcttg tattggttta gttaggattg cttggctata aggagctagt tctttatcaa
    43741 aaccatcaaa gtaggttttc agtagcgatt taactgatcg ctcatcttgt ttgatttgga
    43801 taatttcagc atccaaatcg gttgtgagat caggagcttt tgatgcgatt acctgatttt
    43861 ttttatcttc caactctctt aggctgttta acaagttgta aaaagaatca ttttgattgt
    43921 tcattccaac gtgagttgca attttaacag aagtttcgtc cgtattgtca tcaaaagaac
    43981 tcattggaat atcaagtagt aacgaggatg tttcttttac tgactttttc tttacagcat
    44041 tttttgtttt tccatctttt tttctcttta ctcgagatgt ttttggagct agtttaggta
    44101 aatcacttgt tttatcagtc atttattagc ttttcctttc tattaattta tgttattata
    44161 ttatcataat aacataaaaa agtacacttt ttgggggcgt agttattgaa aaaatctaag
    44221 agaaagattg ccacgttgct tatgttaatg gtgcaattta cagggattgc aatgagtgta
    44281 atcacaataa atccgcaagt gatacatgct gatgggatag ctacttatga tgcacaattt
    44341 aactgtacta ctgaaggtag tgggtcagga acagatagcg atggaaacga tactggaagt
    44401 ggcgctgtat caggtgatgt agcaaaaaat attgatgata tttacaaatt tatgaatggg
    44461 gaatatgggc tatcaggtgc tactatcgca ggtattctag gtaactggaa acaagaatca
    44521 ggtaatgatc ctcgaacggt agaaggttat tacggtgatg gacagagaga agttgaattt
    44581 atcaaacaat tcactgatgg tctgatgggt attggctatg gtcaatggac agcaggtcgc
    44641 catactgcat tagtaaactg ggcgaagaaa aactttggtg gcgcttggtg gacgacagaa
    44701 gcgcaattgg attttatgtt caaaggtgat ggtggatttg ttgctatatt aaaagattat
    44761 gctttaaacg cttctgatga tgttgtggca aatgccgtag cgtttcataa gaattgggaa
    44821 atttcagcag attccgaatc agctgtagtg gctgtacgtg gtggaaatgc aaaagaaatt
    44881 tgggaataca tgaaaagcaa aggcatggac ggagctaaag atgtaagcaa aatcaataaa
    44941 attgccggag gaacaagtac cggtggtgga tcaaataatt catcaagctc agcatcagga
    45001 acagtagaag atgcctgtgc aactacagat gatgctaatt ctggttctgg atcaatgggt
    45061 aaagaaggaa aaattaacgg taaaaaaggt caagtaatag cgaatatgac ttatgaagaa
    45121 gctttgaaaa aatatgggaa agcgatcacg ctaccaaaat tcaaagaacc agattggtca
    45181 acttcacctt ttaacggaat atatcaaggt cagtgtacag agttgacgtg ggcttatatg
    45241 agccaattat acgatgggaa tcaaccgact actggaaatg gtggctacgt atgggaaagc
    45301 tacaaatcgg caggagcgaa aataacgaac aaaccaatcg taggttatgg gtttagtgct
    45361 tcagatggat ggttatgggc tgttggcgat gcagggcata ctggtgtagt tatagcggtg
    45421 tttgaagatg ggtcatttct atgtgctaac tttaatgttc caccaaccca agctccaaca
    45481 aggggagttt cggtaacttt gattgatgga gtggacggat cagataatgt gcatttcttt
    45541 agcggtgtag gaaaagctaa atttgaaatc gaaaaataaa ttggaatgaa ggacgatata
    45601 gatgaatagt aaagtgaaag atacaattat catttctttg ctttctggta tgttattagc
    45661 actgcttgtt ggaatcctat tcttcaataa gcagaatcag gacatagaag ctgaaaaaca
    45721 cagtgtgcat attgaagata agctgacatt ctttaaagag gttaaaccaa atgaggtgga
    45781 tggattacgg aaagatttag aaaatgatat tcaagaaatg ttgaatggat cagttcaaaa
    45841 taagtttgca gatcaatcag gtgaagaatt tttgagaaaa ctatttattg tcacaggtaa
    45901 accaccaatt actgataaga gtagtgataa agaaaaaatc gagtactata agccttttac
    45961 ttttaagcta acaaactttg gaattaagac atctagtagt gggttgtatg aagcattgac
    46021 cgattttgaa gttcaataca atggaaaaaa agtgtatgat accacattaa ccatcacact
    46081 aaataatgat aagaaagtga ctgggggtgc tttttatgca aaatcatgaa atcactactg
    46141 ctaaaaaggc gattggaata ctttccataa ttgtgatcct ttgttgtgtg gtactatttc
    46201 aaaagaaaaa tgcttatgat aataatgtca aagaattgga agaccttaaa agtaaacaaa
    46261 tcgaagtagt caagaataac cagaaagtga aagctatgca ggaagcccaa tacaaagaat
    46321 tggggaatgc tgaaatcaag gaacaaagta ggaaattctt taatttattt tacaactgga
    46381 attcatggga aaaatatacg aagaatatga aggaaataca aaaagaattt cctcagatag
    46441 taaagtctag tcctgttgat atttctggca cgatggtagg cactggtaat agtccaatat
    46501 ctagctatac tgctgattac tatacaacaa atcaaaaagg taaaatggtt gaattgatta
    46561 cacagacaag aaatactcca acaacagcaa caacaacaat ttggcgtgga gtaacaagca
    46621 taggagaaaa tcaattgttt tacttggaga atttagatag ctatgtaaga gcggagtaaa
    46681 ataaaaaaag agctaacggt accaacgtta gctctttgtg ctgacgatga taacaaaacc
    46741 taccgaaaaa ggattgtttc atcacaaaca caaacagaaa agaattgata aaaaacccga
    46801 acctcagaat ttttattaat caattcattt agattataat aaattgagct gaacattaca
    46861 atacgtatat gttgtaaaac gtaaaaaaag tcaaaagtaa atactttctt aaaaagtcaa
    46921 ataaaactat taaagacaat atatatggtt tatttgacat aagaaaatgg tctagatgat
    46981 aattgtaata tagaattgta ggcttaaata aagcataaaa taccttttat gataaggtat
    47041 agctcttaat tttaagcagt ttttagatat aagagtgaaa gtaaagtggg ggagaataat
    47101 aatggcagat cgtaaattag aaaagttatt agaagaaacg tggaatccaa aagaatttag
    47161 tgaatttttt atggagaatt ttgaaactga cttagcagtg attgttaaag atgctcttag
    47221 agagcaaggg tatcctgaaa ctgcaaacta tataaacatt aattttactt tgtatactga
    47281 aaataaagga acttgggatt tttgggctac tttagcaaat aaggagttaa gtgataaatc
    47341 agacacagga attcgtaact tttttgaatc aaacagagat gattatatgt atgctaacca
    47401 tcaagataaa ttgaacttta gagtagagtt tgatgaaaca cctgaagaat ttatagaaag
    47461 acaaccgcct aaagaaaatg tagcaaaggt attggaagat cgttggaatt cagatgaaat
    47521 agtttcaaca attagcgaac taggcggaca atatgaaccg ctagtagaag cggttagaga
    47581 agagctaaga ctgaataagt ttcctgatgt tcaaaatatt gatgtttctc aaattgaaat
    47641 aaacgtcaag atcacgaata aacttgatta tggtagttgg gcagatatag cattagaaaa
    47701 atacatctac tcaacgttaa aagagtttat cgaaaataga atggatatca tgtatctaca
    47761 gcatccacag tatttaaact ttggtgttga aatagctaca cctcttgaag aatggaaaat
    47821 ggagcaaggg cttgactagc ctttgctcca tttttttatt ttacaaatga atacagttcg
    47881 tcgtttttac caagaatgat tgtttgccca actttgagat tgtcaggtga tccttcagcg
    47941 gtttgtaagg agaacgttcc gttgaactta tcggataccg ttataggtag ctttaattct
    48001 gatccttcct catctttgag aattgctgtt tttcgttcat caaaataaac agtctttatt
    48061 aaataatttt tcgtagtgat ctcagcagat ttgtcttttt ttgtagtagt aacagtttct
    48121 ttagaattag aggtattttc tacgactatt ccatctttgg catttgagct tgtgtttatt
    48181 tgattgaata ttttcagcga caagataccg ctaagaatag tcaatataga aacaagtgta
    48241 attaatactg ataatttcct ttttttgttc attttttctt aacttccttt ttgttttgat
    48301 tagttagata ttccttatct aacaagcctt tttaggctat taaatgagga taaaccccaa
    48361 aaaaatattg caattatgac agtgcaatgg tactattatt atacaataaa ttttgcgaaa
    48421 ggggtaggtc taaatcgcaa acaaaaagaa attgaaccta gacaaataca acttgtacac
    48481 cgcaagggaa gcaagtttga ttttgggtaa aggtagaaat tatgttacaa acataatcaa
    48541 ctattatcca aatcgtttgc ctaaaggttc gttcaagaaa gtgggtgaca aaacctatat
    48601 tataacggaa gaaggaatag aagcattgcg atcacaaaca gaaaataaaa acaaaaagaa
    48661 ggaatgaata tgaagaaaaa ttcgcttggt atggttttat ttgcatcagc tgtacttgcg
    48721 gggttatcag taccagtaac ctcacatgct gatgaatgga ctgcaaacac accggatcaa
    48781 atccaaatta caacaggaca atcaagttat gcgttcaaaa aaggagatac tatttggtca
    48841 actgtagaga agcttaaaag cttaggttac aagactactg ttgctgagtt tgccgaattg
    48901 aacaatgttg atttggcaaa aggagaacaa cggactatcc caattggacg aactttcaac
    48961 atcagtaaag atacattaac agttaaggaa gcagatggcg aagtagttag tcaaactgtt
    49021 caaaaagata aacctgatcc tgttgaaaat acggagcaag agaacaaaaa atccgagact
    49081 gcaaaagaat cggaaccaac agcaaacact tctgataaga cagaaacaag tgcttcaagt
    49141 gttgatggag caagttcatc taatacaaat aattctacaa gtgagaaaga agcatcacag
    49201 ggaacaagtg aaagtagcac tacggaaact gatactggaa catcaacaaa taactcacaa
    49261 tcaacatcaa atgatgcaaa tgaatcaacc agtacatctt ctagtgatca atcacaaaca
    49321 ggttcgagtg atgaaactga cagttctact tcaccaagta aacccgaaca accaactaat
    49381 gttgtgctag gcgttccatt tattagtcaa ggtaacacaa tgctttgcga aggaacatct
    49441 ttgctagaag cgttacatta taaaggtgtt gcaacaaatc aagatttaat gacttttgtt
    49501 aaatcaatgc cactatctcc tgataataat ccaaataatg gtttttctgg tgaatggaga
    49561 cacaatgtgg atggaactta tcaaggtatg aatccggctc ctgtagtttc atggggtgtt
    49621 gctaatggcg gaaatgttgt agatatttct ggttcatcgg tacaaggatt aaaagatgag
    49681 attgtaaatg gaaatcctgt agtagcgtgg gtaacttatg cctttgaacc tgctcaacat
    49741 aaatcaatgt tttggggcga tgctatttgg aatcgacatg tagtagccgt tgatggattt
    49801 aaagacggtt tttaccatgt tgtcgatcct gtgtttgggg caagttggat taatgcagga
    49861 tcatttgaaa cagcatataa cgtatctcaa ttagcggttt cggtacgtta ataaaagaga
    49921 ttgtttatat actaagtagt taagtatagt ctttatttgt ccttgcttaa ctacttagaa
    49981 aacttgttgc tcatggtggg tagaactaaa aggggtgatg ctatgaatac tagagcgttg
    50041 aaacagcttg ctttcaacga aaaaagtgat taataggtgt ggtgatttta acttaggaat
    50101 gggcgcgaag gtcattacca caccgacata tcgtggctca ttttcttcgc ctaactggta
    50161 taaagaagga gtaacgagag atgaagcatg aaaaaacgat aattttaaaa tcaaaaataa
    50221 tttattgttt tgcgacttta gtaatggtgt tatctacgct tataatgcct ttttcagcaa
    50281 taagagctga agcagatgtc acaaaaccga caagtgcaaa atttacagaa actggcgtaa
    50341 aaacagacgg ttatattagg gttaatatgc cgaatcatcc aaatgaatgg atgatttcaa
    50401 gtcaatttaa agattctcat ggaaatattg gatattgtat ggattcagaa ttgccatccc
    50461 caacaggaag tggagccgga agcttgaagt acaagggagc tggcagtgat gagttctatc
    50521 gtatgtttaa aggtggtttt ccatcaaaaa cagcaaagga actgggtgca ggaaatgata
    50581 cagaagcatg gtatgcaaca caattagttt catgggtttt agccggaaac tttaaagttt
    50641 cacagattgt atggtcacat ccaaaccata ccgccgcaga aacagctcgt gtaaaaaaag
    50701 catttgaaaa aatttatgac tatgctaaga atggaaaaga tacgcccaac actgagtttt
    50761 ctatcacagc ttctaagaca gctgacgaag gaaaatatca cactttcaca tataaaacag
    50821 catcaaataa gactggtaat gcgaagctca cttttacatc agcaaaacca gctggtatga
    50881 agatttatga tgcagatggt aaagaaatta ctaacaacac tgtgaaatta aattcaagtt
    50941 ttactattaa agtacctgtg actacaccaa gtggtacttt gtcatttaaa ggtactgcta
    51001 atgttagcac aaccaatcct tttacttttg atggacgagg cgtgtatcaa gatgccgtag
    51061 ttatgatcac tacttcagaa acaaaggact ctaaatcttt gagtgctaaa tggacaagag
    51121 ctgttggtca ggtaacagtt aagaaagttg atcaagatgg aaatgcttta aaaggagcgg
    51181 agttcactct aactgacaat aacaatgcta aatctgttaa gacaaccgga gatgatgggt
    51241 tagctacgtt tgatttgcaa caaggcttaa catacagctt agaagaaaca aaagttcctg
    51301 aaggttatca aggttcattt aaaaaggatg gtattactgt taataccgat ggacaaaaat
    51361 ttagttacac tgctacgaac acaattaaaa aaggtaaagt aaaagttaaa aaggttgacc
    51421 aagatggaaa accattagct aatgctgaat ttaccttgac ggatgattca tcccaaacac
    51481 aagcagaaaa aacagatgaa aacggacttg caagcttcga tattgtagca aacaagacct
    51541 acagccttga agagaccgga gtaccaactg gttatacagg cgcattcaag caagaaggaa
    51601 ttaccgttaa agatgatgga caaacttttg aatatacagc tacaaacact ttgaaaaaag
    51661 gtgtagtaaa agttcataaa gtcgatcaag atggaaaacc tttggcaaat gcagagttca
    51721 ctttaaccga tgaaaaaggt gcaacacaag tagaaaaaac agatgaaaat ggacttgcaa
    51781 gcttcaatat tgtagcaaac aagacgtata gtctagaaga aaccggagta ccagtaggtt
    51841 acacaggtgc atttaagcaa gagggtatca gtgttaaaga cgatgggcaa acttttgaat
    51901 atacagctac aaacactttg aaaaaaggtg tagtaaaagt tcataaagtc gatcaagatg
    51961 gaaaaccttt agcagatgca gaattcacct taacggatga aaaaggtgta aaagaagtta
    52021 aaaagacgga tgcaactggt ctggctacgt tcaacattat tgcgaacaaa acttacaact
    52081 tagaagaaac aaaagtacca acaggttaca caggcgcatt caaacaagaa ggaattaccg
    52141 tcaaagatga tggacaagtc tttgaatata cagctaaaaa cactttgaaa aaaggtgtga
    52201 tcaaagttca taaagtcgat gatcaaggga aagcattagc aaacgcagaa ttcactttga
    52261 cggacgacaa aggtgtaaaa gttgtacaaa aaacggacag aaatggcttg gcaacattta
    52321 atattgttgc caacaaaacg tacaaaatcg aagaaacaca aaaccctaat ggttatacag
    52381 ggaaatggtc taaagaaggc atcaccgttg ctgacgatgg acaaaccttt gaatatacag
    52441 taattaatca ccttgtacct gaaacagtaa gaacaggcgg aatcggtaca tcagccttcc
    52501 ttcttgtatt tctaattgca ggattgagta tggtgctact gtattatcga gaacagcaca
    52561 aagcaaaata aaaaagtgtt aatggaaaag aagtaaatat gaaaaaagaa ggctgacgta
    52621 tgaaatcaaa aaaaattttt tcaaatttaa tgctatttgg tactttgcta ggagtgggat
    52681 tgggcgcaag ctcaatctct tctagccttg gactaggggg gcaagtagca tatgcacaac
    52741 aaaactataa ttatcaagga actggaacga ttcatatcca taaagtaaca aatactaatg
    52801 gggatgggaa ccctgtctca gatattgata acaaagggaa agaaatagct ttaccaaatg
    52861 gtgtaaaacc tcttgaaggc gttgagttct cttatgtgaa acttactaaa gatgaagcta
    52921 aatccttggg atggacggat agtgatacta caaaaggtca acatattgat gatgctaaag
    52981 cacgattatt actaaacaaa aaatacagtg aagtaagtag aatgaagact gattcacaag
    53041 ggaatataga catacctata caagtaaata cagacgattt aacagataac ttgtatttga
    53101 ttgttgaaac aggaacagtc aaaggtgtag ttgaagaagc gtatcctatg attgttaacg
    53161 tacctgaact aaacggatca gatgatcaat actttaatta tgatgtacat gtctatccta
    53221 aaaactattt ccaattaaac tcagcagagc ttgataaaaa agacgaagat ggaaaggctc
    53281 ttgccggaac tgtttttgaa ttgcgaaaag atgatggatc tgatcaggtt gttggaactt
    53341 ataaaacaga cagcacaggt aaattaactg tggatggttt ggaattaggt gactattatt
    53401 tcctagaaac cgatttggga acagatgcta atagaacaac ctatttacta gatggtaaaa
    53461 aattcaaatt tagcgttaaa cgtggggaga ctgctaaagt agttcttgat gttaccaact
    53521 acaaacgacc tgtaattact aaggtaattg ataaagaaac cgttgatact ggcgaagatg
    53581 ttcaatatac attgaaacca tcaattcctg gtaatattgg taattacgaa aaatatgttg
    53641 tcaaagatgt tctaggtgca gagttaactt atgattcatc agaagtgatc gaaggaatga
    53701 ctttagatac tgattacagt atcgaacgtg acggacaaac gacaaactat gtctttactg
    53761 aatcaggtat ggataagctt cagaaacaat ttgatgcagg ggatcgcttc tttacaattt
    53821 tgatgaacgc tcatactaat agtaaagcta aaatggatac accacttaag aatgatgttt
    53881 caacgatcta caacaatgga tatgaacccg aagacgaaac accagttgat cacgcttata
    53941 caatcattgg tgatcaccaa ttcattaaaa cagatggaga tacaagcaaa gcattgagtg
    54001 gagcaaaatt tgtagtgaag aacaaaattg atccgaataa agttacttac ctaaaacaag
    54061 atccaacaac gaaagctgtt acttggattg cagataaatc taaagctact gaattcgttt
    54121 ccggatcaga cggtaagttc caaattgaag gtttgcaata tacctatgat gtagattttg
    54181 ataaacaagg taatccaaca atcacagata aaaaaatcaa tgattatgct cttgaagaag
    54241 ttgttgctcc ttcaggatat acaccaacta gaaacgatgt tccatttgtg gtatcgaatg
    54301 atgatgcttc tagcgaagta acagacatca agaaccttaa aacacctgaa acagttcgta
    54361 caggtggaat tggaacagta gtatttctag tggttgcagg tgtggcagga acagggctat
    54421 atgttgtcta caaacgagaa caacacaaag cttaatgaaa taaaattccc tagagtgttt
    54481 ttggctctag ggtttctttt taaaaagtgt gtgaaaagta ggtatgagca tgaaaaagaa
    54541 gaatatcaag ttgatcatct taaaaattgg gatgttgcta tgtttaggag taggtctata
    54601 tcctgtaatt actcaggctt atacctacta ccaaactcaa caaacactaa actcctataa
    54661 ggaaaaatat gatgaagctt ctgaaaagga gaatgaagaa gtcttcaaaa gatattcaga
    54721 agttaatagg aaacgaaaag acacagaaca aacctcagtc acgattcctg aagataataa
    54781 caatattcct tcaaatggaa atcttcagaa ggataaatta ggtgaattag tgggtgaaat
    54841 tcttatacca aagattaatg tggatttgcc aatttatgat ggcgtaaacg attttcaact
    54901 atcagcagga gcaggaatat taaccggaac tgattatcca actggtggga ttggaaacca
    54961 ttctgttata tcagctcatt caggcattcc taataaaaaa tattttacag atttagagtt
    55021 gctcaaactt ggtgataaat tttttattga tatttcaggg aaaaaacttg cttatcaagt
    55081 agatcggata aaaacaattc taccaaccga atttgaggat ttgcttcctg tggcaggaaa
    55141 agattatgta acacttatga catgtacacc tgttggagat aattctcata ggttactcgt
    55201 tagaggggtt agcatacctt accaaaaaag tattgttgag aaagaaaaga agaaagaaga
    55261 tttaaaggta aaggcgcaga tgaactggta tcctatcgta gttgtagggg ttttattaat
    55321 aatagctgtg atagtcatta ttaaaaaaat agtagcaaag agacgaggaa tgaaaaatga
    55381 agaataaaat actacttttt ttgactctta tttgcacttt ggttttaaca atttatcctg
    55441 caaatcagct ctatcatcaa ttcaaaacaa aagagaatgt ggcggaaatc caagagaaaa
    55501 ttgagaaaaa acaaactcct tcaaaagagg aagtggcaaa aaaagtcgaa caaataaaat
    55561 cgaacagcgg taaccaagga ttagacaata ctcagaaaac agataaagga gtagacaata
    55621 ctcaggaaac agacggtgga tcatttgata ttcgtgaagc tgaaggaaag gtcaatccat
    55681 acacaggaga tgttttttca aaaaatcaaa cgaaagattt tgaaccatct acctattcag
    55741 gagcggttgg ttatgtctat attccaaaaa taaatgtgct agagcttttg tttctaggcg
    55801 cttcacagga gcatttatat agaggtgtgg cacaaatgag tgctactagt ctacctttat
    55861 caggtaaagg tcatcaaaca gtaatagcag gtcatcgtgg cggttatacg tcaccacaat
    55921 ttctttatgt tgatcaatta gtagccggag accacattta tatagattat cttggtgaaa
    55981 aagcagttta tacggttttc cgaacagacg ttattagaaa ttacggtcag aattcaacac
    56041 tgactgatat tcctgattta gatatgttaa cactttatac ttgtcatcca tatcctcaga
    56101 actacgatcg tttattagtt agaagtctaa gagatgaaaa agcaaaatac atcacgccac
    56161 aagtaacagt tggcaatacc gcatacgctg atgccattga tagtgaatcc gtttcagata
    56221 agccagaaac tgttacatct tctaatgatt tatttgagga tgttgatcgt gccaacaagg
    56281 aattagatgg aaataatgat cttaatattc cggaacaagg tagcgtagag atgcaagaca
    56341 gaacgtttgc tgaaaaggca agttcaatat ttacaggaat taccaataaa catcagaaaa
    56401 tgcttttagt catttggact gtattaggag cattagcaag tctaaaggtt cttattcttt
    56461 taatcaaaga gtttagaaaa gcataatgaa gattataaaa attgatagag tacagtaaaa
    56521 agggattgat actattattg atctcttttt tattttgaat agagctagat tcacttttaa
    56581 ataaaatcaa aactgatctt aattccaaag aaaaagtcaa gaaaaaaata caacaaattt
    56641 ttttatcaga atatttatca gaatattatt agaaaagata aactcaaaag cttaatttaa
    56701 tagggttatt ggttgtattt agaaataatg tatggaaaag ctataaagca atagataaga
    56761 tgtagaaaat cttatttgtg aatgaaaaac aagtcatcgt aaaatattaa taggaggtgt
    56821 agtaatggtg gaaatatcaa aaacaacttt agactcatta gctaaactat ttagtagcag
    56881 gtttgatggt gcagaatttg ttgaatatct aagccaaaac gaaagtggaa aaactctgat
    56941 cgaaacactg aaaaaagact atttgaatga aaatgacttt ccgacatgga cgaatattga
    57001 agatgctaca tgggatgtag tgtgtacgat atattctcct tgggtaacga ttgctgattt
    57061 tgttttagat aaaattggag gggatacata ccctgatgcc aaatctcttg ctacaagtga
    57121 ctttaaaacg ttctatggtt atctaatggg tctaataggc aatggaatag atttgaaagt
    57181 tacgccaaaa caaacacctg aagaaattga aaaagaaaaa ggagaggttc aaatggctta
    57241 tgcagatgac agagaaaaag taaaattatt ggaaggtaaa ttatctgaag aagaacttgg
    57301 aaaaatggtg caagaaatca atgaagacag caactatttt cctgaattga aggtatatca
    57361 aaacgacgaa gatttcttta atgaaatgtt tagtaatagt ccatgggaag cagtgagtaa
    57421 aacaaattta ggagaatgga actttcaaga tgatctagtt gcatttagca atgatgggta
    57481 tttacgatca tattcagaaa gtgagtatag agctgaatta aaaacgtatg aaacggatat
    57541 tctgatgagc tatttaaatc tattagagag tggtcaggga gagttaagag ataaagttga
    57601 agaactatta aaagaagagc tatagggatt atttcaacat aaacacaagc agaaaactac
    57661 caaacaaaac gtaccatgca aaaagccggt taaaccggct ttttgctcta ttatgagagg
    57721 agatcaaacc atgagtgcaa caaccgatat ggctttagaa gcagtaataa aagataatac
    57781 aggaaatgtc gaaaatgtag aagactttag cgatattgca atcaatcttt cagcatggat
    57841 ttcagctgta ggagaagaaa atatcgaatt tgagatcaag aatatggatg acttaaaagc
    57901 aaaatttcct tttatcgcat cgaaactaag tcaatttgcc gaggagaaat cgtggaatga
    57961 attagttagt ttttccgaaa aaatttatga tattacgcaa gaagatgagt gtgtttcatg
    58021 ggaaatgaat aatcaataca agaaaattga tgctttcgtc aaatcacgta tagaaggaac
    58081 agcttttgag tttgaaggta tagataccgc agataattgg ttaaataaag aatcctttat
    58141 taaaagtata gcggatgatc ttgaaaaaca atggtctaag gaagactatg cagagtacct
    58201 aaaaacagaa agagatacag aggtgaaaga aaaactcact gaggtatgtt tggatgaaca
    58261 tattgatccc aatatcgtaa aacaagaaga ctatgaagtg gttattgata aagttcctac
    58321 tgattttgag gaagttgtaa agtatatagt tgaagaagca ttatcgcaat actcttcaga
    58381 tgaggaagtg gacgaagaaa ctgttcgtac cagagtttgg caactggttt ttgatgacaa
    58441 agattataaa tatttgagtg atcaaaactc ggttaattgg agtgtacgat tattaattga
    58501 tcaaaatgat attgaataaa atagagctga aaagctcttt tttttttgat aaaaaaacta
    58561 gctatttttt taaaaataat ttggttcaaa atttattttt ttaacttgat aattttttgc
    58621 catccttttt taaattggct atatatcaag gtttatagtg gggataaata aaaatacatt
    58681 tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac acaataaaag
    58741 gaatgatggt atgaaaaaga agaagtttag tcgatcgttg ttaggaattt tagttttagt
    58801 tgcggttggt cttagtggca agggttactt tcagaataac agcactcagg aacatgattt
    58861 tcagatcaaa agtagtgttc aggtaggtaa taaagcagac agtgaatcaa aagaattttt
    58921 agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta atgatgggaa
    58981 atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc aaaccttttg
    59041 ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag cgttgatcac
    59101 ctatgatgct gtaaattctc actcatctaa aaatgtggaa cgtccatctt tcaaaagttc
    59161 tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag atacttgggt
    59221 cggcaaagat tcaaacaatg aaatcactca gctagaagga tataaaggtt atctctataa
    59281 caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca atttaacact
    59341 tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg cttactcaga
    59401 aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt ataaagttga
    59461 tccaatttat aaagggaatg aattggttcc tcgtggttct catgtcgaag ctttctcagt
    59521 taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc aaaaaggaat
    59581 caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa ggtgaagaat
    59641 tggaaataat aagcatcaat ggattagata aatcagttgc aaatcgaata aaaaaaggaa
    59701 atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta gaaacaatga
    59761 gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt gatccagatt
    59821 ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa ggggcgatga
    59881 tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat acgcagatgt
    59941 gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga gatattcaga
    60001 tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa aaatattatc
    60061 ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta gttggtgcaa
    60121 ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta gataccttag
    60181 taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga ttaaaagaaa
    60241 tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca tataatgtac
    60301 aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta gaagcaggaa
    60361 cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat ggagaacaaa
    60421 gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta gaagctatgg
    60481 atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa aaaagaaaac
    60541 gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc aaaatttctg
    60601 ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc gtaaaaatcc
    60661 cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa gatttagggt
    60721 cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa gatgaagttt
    60781 caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt aaggaaagag
    60841 taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt tcaaattcac
    60901 aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa aaccaaaccg
    60961 aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat ctatcttctg
    61021 ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt gaatatttgt
    61081 ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa attgaaactt
    61141 acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa attactttac
    61201 cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat agcgtgtttt
    61261 aaaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac agaaaagtac
    61321 cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa aaagagagcc
    61381 gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa tggaaaattc
    61441 agtgtcaata tgttgaacac aaaggctaaa gacccgacag atgctatttt tggaattaag
    61501 cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt ggttgcagga
    61561 acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagc aatgtgtcac
    61621 tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga atttggaaga
    61681 tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga agcttatgat
    61741 ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga aaaagcaggg
    61801 ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga tagatttccc
    61861 tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa agaagtagaa
    61921 ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt tatcttagct
    61981 acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt tccgtcacgt
    62041 gtttgcctta tggtagcaag tgatttagaa agtaggatcg tattagatga aggcggtgga
    62101 gaaagcctaa gtccaaaggg agatatgatt attaagctcg ttaatggaag tatggttaga
    62161 gcgcagggag cttacatcag taacaaagaa attgaaacaa ttttcaatca ccttagaaat
    62221 actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaatc tcgtggtgag
    62281 ggtggagaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa atctgctaaa
    62341 agaaatttga acacaccttt agcaaattca tcatctaaag agccatcaat tgaaaagaag
    62401 agtacgtttg ctctaaaact tgaaaaaaac aaagaagaaa aagaaaaaca aggagctaaa
    62461 acactagaaa aacttcaagc cgaaaaatta gaaaaagttt taaaagcatt agataaagct
    62521 gattctgaac cgcaaacctc aaacaacacg aataatgaac agccgatttc atctaaaact
    62581 aaaccgaaaa ctactgatcc gaaagtttca gtaaacgtga ccatcacaac cgataagtct
    62641 gagaagaaac aagtaaacgg aacgacttca cctatcatgc aaaaaatgtt gaaagccaaa
    62701 aaacaggtgc ataaatgatc attgtcagtt cttttttgag gtggcttacg ccatataaat
    62761 atacagatga agtagttgtg aaacgaacga agaaaagaga taaaaaagcg gtactaactc
    62821 ctaaacagat gcttgatcat ggcattttag gggtgaaggt tttagaagtg aataaaccgt
    62881 ttgaatcaaa tagtgcaaga gaaaagttgc ttggagctga agccgatgtc gcatattttt
    62941 accatgacaa aagcaagcat caaatagcta ttaaattcaa tccaaaatcg gcatggcggt
    63001 atcaagcaag agatgacttg aaacgtagga aacttagacc aatgctctat ccagaaggaa
    63061 aagaagcatt tgatagaacc cacatcgttc ccattggttt tcatggtaca gaatcagata
    63121 accgcctatt agtaggttgg aacagcaatc aaaatagagt tcagatcaat aaatttgaga
    63181 agaaagtaag gaaattcaac gatcgaaaaa cgatcctatg gtttgcagat atttgtaaac
    63241 agccggatgg aacagcaaag tgggaagcgg tgatatttga cgaaaatggt aaaaaagtaa
    63301 tgtctgaaac atggcatgat aaagctaaat ttgtttggca gtagcagtag aggtgaaaaa
    63361 gattgctaga actttttgaa gcaaatagaa caggctttaa tcaaaaatta gaagtgggag
    63421 aaactataga atataaagat aaaaactata ttattatcgg aatctataac acgagaatta
    63481 acagaatagg tgatccacgt ataaccagtg acttggtttg tcaatctgtt aactattcac
    63541 ctgatctgac aagcaggtat aagaaatacg tgttattaga atatagacac aagattactg
    63601 atgaaattgg tgtaaacaac acacgaaaca tatttaagat cggaacagtg attccttata
    63661 gtaacaacga agaaaaaata ttttatcaga tttacggaat agaaaagttt gaatatgaac
    63721 atgtcgatct gctagtgacg taccaaatgc gtttaataga accttggtca caggcagaaa
    63781 ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga aatgcctttt
    63841 aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg taggtaagaa
    63901 catgacaata aataataata accctaaaaa atataatgct cttgtccaat gctatatcga
    63961 aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaga gcctagtatc
    64021 acacgatttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa aatttcaaaa
    64081 gagagtttat caagaaatag gtaagtggat tgatgaaggg aatgcaattg accctttaat
    64141 tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa aggttaattc gtcaaggcga
    64201 tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa atcttcataa
    64261 acaaattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa aaaaatacaa
    64321 tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat cggcagttac
    64381 taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga tacctagtag
    64441 cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag attggaaaaa
    64501 gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt ttgtatgaag
    64561 gacacttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg taccatttaa
    64621 tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcag tatgagtggt
    64681 taacattatt gatcgtttta atcatatcag gatttctagg aagcttagtt ccttttctgc
    64741 tacacagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg attgctttat
    64801 ttgcgattgc atacatccat tttgatagca agaaacattt attactaagt gtttttgtat
    64861 tattgataat cggcatttta ctggtatcta cttttgaatc aggcgagaca aacgtcttgt
    64921 cacatgtaat cggatttgtc tgtgggatga tcttgggatt tattctaaag aacgtttcat
    64981 tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc aaaaaaaaaa gaaaagaaaa
    65041 ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta agaaaaaaga
    65101 ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa cgtgaagcgc
    65161 taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa gcacgtacaa
    65221 atcacgaaga cgaaagcaaa ttaggattag ctcaacaacg aatatttgaa tggctgaata
    65281 cgaatactga gcaagaaccg ttaagagcaa ttagtaatct ttttgaattt ggaacgaagc
    65341 attggtttaa cgctgtagaa aaggatgctg acttacaaga agcgttcgat acactaagcg
    65401 aggatagcaa atccatgcga gacttattga tcacttatct aaatgagcat gaatttgaac
    65461 agacgaaagt taaaaagaaa aataaaaaat agaaagttgt gatgtatcat gctgtttacc
    65521 ttattaggtt atttatgttt agttcttgct ttttttggag caatagctat atttgtcggt
    65581 cagtttcaag caaataatcc gataatgcgt aacataggca ttggaattgt tatcatatcc
    65641 ttgattgtat ttggtgccac aaagattttt gggagtaatg atcagcagtt tggtatttct
    65701 ggagttgata atagttatgc tccagattca ttgggaagag tagtgataga tggaacgttt
    65761 gagaatgtaa cgagctacaa aaatgtttca ttgacagtga aagaagatga caagaaagca
    65821 tcagatgacg ttctcgctac gatcaagccg tctcaatcac agtttgaagt gttttataca
    65881 gtcaaagaga ataaccctgt acgcaagcta acattagttt ttaacgttga cggagaagaa
    65941 tactataaag atgtaaaaat caataaagca tcaacgaaaa agattgtcga atcagcgaaa
    66001 aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga agttgaacta
    66061 ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt ttctggttct
    66121 atggcagatc ggtttaacac tgaagttttc aaagctatca aaactgttaa attggatact
    66181 gaaaagcaaa ggattaatgt taccttattc aatgcagttg aatctatgga tcaggataaa
    66241 aaagttacgg ttgcaagaga actagggaaa agagtaattg ggttgtatcg aggtacggca
    66301 aaaccagatt cagacggttc agatttgcca gttcacgtag tttatgaaga cggtaaagtt
    66361 tttggagact ctacagcaaa tgaagcacta acgattaaat tagctaaata gccggaaaat
    66421 tataataaac aagtggtgtc acaccaccat cgcataggtg tgacaccttg gagatgatta
    66481 ctgatggtag taaataaaga aaaagtaaga aagtatgtta ctttaaaaat agcttctgtt
    66541 gaatatttag aaaagcgagc taaagaagaa gaaaaaacaa gaggtaagaa ggtaacagtt
    66601 tcagagttgt tagaagaatt aattgagagt gaccaaacaa taagacgtgc atttaccaat
    66661 aggtctgtta aaagttaggg tgatactatc tttaatttca accaaatttc taatatatga
    66721 tgaatagttt ccttgtacaa aatatctaat taagagataa aagttaggag gattcattca
    66781 aaatgattat ccaaaagttt gactggaaaa tgaaattagg aattagtctt gtaatattgg
    66841 gatttattat ttttttgggg ataaaagcac aaaaaaatca atctactttt gaaactatgt
    66901 cttctcaaat tattgaggaa tgttccaaaa tttattattg tgagaacaaa actgaagaat
    66961 ctaatggctt ttattatatc gatcaaccac atgatgttgt tataaaaatg gagtcacatg
    67021 aagtaaacga atggattcaa agaagtacta atgtatcaaa aaagttagaa agatattctc
    67081 aaacgttttt aagtaaagtc attttttcaa attgcaaaaa taaagaaacg tctcaaagag
    67141 tagctagttc tttagaaaat ttaaaatttt ctgtttcaaa aaaagagaaa tttgtagatt
    67201 tacgaaaata aactatcgaa agtagtatga ttatgatctt aaaatatcat cttaagagaa
    67261 gaattacgat tgtttatagg atttacaaaa tgaataagtt tgaagagtga tttggtcgca
    67321 gactagatca ctctttttgt ataaaaaaac tttacactta tttttgagtg tgttaagatt
    67381 ttttatatag agtgacggtt tcttgtcaga gtgaatgata accatcaata ttggaggaat
    67441 aataagtgga aaacaataat gatattagat attttagcat tggggaaaga gttacaacag
    67501 taagtgaatt atcaaaggat tatagagata aagaaggaac gattatttct cttaaaaaga
    67561 atcgaagagg tgtttggcaa tatgttgttg atttagggga tggcgttaca gttgagtgta
    67621 aagatagtca actgatcaga aatgatggca gtcattccat tgaaaaagca attcttgtat
    67681 tcccaaaagt atcaagcaag attatgaatg actggttgct tggaaaattt ggtgagtaca
    67741 ataaaaaaga aatcgcagaa gtgatcggca aatcttatcc aacagtatta gggtatctaa
    67801 aaaagataac gaacaaagaa gcgaaagttg aggatgtttt aacagtaaga gaacttcagt
    67861 cattaatcga atatcggaat aaacaaataa caaatcctga gtaccttaaa aaagaagatg
    67921 gtctattgaa acaattggaa caggtaaata aaatccggaa aatgcaaaag gtagctcctt
    67981 taagcctaga taactttaaa aaaatctatt tgaaagagat cgaaaaagac taatgtggag
    68041 tgtcacttta ccgtttcaac ctgtatctgc accaagacca agtgcaagaa ggaatgttgc
    68101 tgatgcaact aatgacgtag cagagaaaag gatttcaacc tattatgata aaaagtatct
    68161 cgattatttg aaaaaaataa aaagctttat cgaagatcaa ggtttgttag atgatgaatt
    68221 ttactcgatc gtaaatgatc cgatgggtgc aattatggaa gtggactttt actatcagat
    68281 accaaaaagc tataaaaaag tctataacat tatgaagacg acagcgccag atttagataa
    68341 tttagtaaaa agtgctatgg atggtatttt caacatctca gctatacgag atagccatat
    68401 cacagggtta attgctttta aatacaatgt ggcaaacgaa ggaaaaacag ttgtgaggat
    68461 caagcgatat tcagaatttg agtgggatac ttctgaagga ttgaacggag atatttggga
    68521 agccacattt gatttcaaac cagtagcaac gccaagacca aagtctaaat ttagaggaaa
    68581 tgggattatc acttactatc caaaagagta taacgactat ttggaaaata tcaagaatac
    68641 gcttaaagag aaagatttgg taaatgatca actgaaaaag gtaatgaatg ctcctatggg
    68701 ggttatagct cagatagatt atttttacca agtgagaaaa gataagaaaa agcttgattc
    68761 actgatgcgt acttcacagc cggatattga taacttagtc aaaggaacat tggacggaat
    68821 ttttaacaag gaaattggca tcaaagatag cagggtggta ggtttaatag cttttaaata
    68881 caatactttt gaaaagtcga aaacaaacgt gcgactacag gagatgggct gatgaataat
    68941 aacgaaaaaa ttcttcatgt acttgatagc tttgaaacaa ttcaagagga actaaagaaa
    69001 tatcgagatg ttttggaaca aagatatgat tttgtcaatc agcaaaagag taatcacatg
    69061 gatttcattc tcaatatgaa tgacttgaag aagaaattag ttgaacgaaa agaacaagaa
    69121 aagttgatca aagcttactt tgaactcggt gaaaaagaag ttaaaaaggc aatggaactg
    69181 aatgaagatc gcagggtttt agatcagctg ttagaacaat tattggtgat gtttcaaaaa
    69241 ggacgaatag atgaggattt aattgaagaa gggttgagaa agtatcctgc aaactcaggt
    69301 atcggaatag tgctgaaagc gattgatgaa gaagaaattg aagatttcat accggcagag
    69361 gattttgaat cgggaatgga atatataaaa tattacagcc aagggatcac tgcttttaga
    69421 gagtttgatc ctgaagatgt tattcatgac ttgaacaacc taaaagaatg gtgtgaaggt
    69481 tatgaagttg atgattccgg tttggactac ttgatcagta ttatggaaat cgaagaagag
    69541 atgccggata agcctgatcc aacagatatt ttagaattaa tccatgatgc tagaaatcca
    69601 attgcataca tttctagagg atatactgta ttggaatact ataagcctta tatatcagcg
    69661 atgaaccatt taagacgtgt attaagggaa aaacgtgaat accgctcagt gttaaatgca
    69721 tcgaatagat tagaaaaagc tgtttcagag ctagatgctt attatcgtga acattacctg
    69781 caagcaggtg gtatgccaag aaatacgaaa gcaaatatca gtaggtatat aaaagaagct
    69841 gagtaggcgg agttagccca taaaatggca aaaggtaacc tgaaaggggc ggtttgaatg
    69901 aaaagggatg tttttgaatt gtacgtatta gaacgtctag aagacggcat gatggcacaa
    69961 gagataattg atgaaattga tgatatgatc agcagatttg gtcaattgga attgaatgat
    70021 ggaaacaaaa attcggtagt gctaaaacaa ttttggttaa ataatttttc gtataaggat
    70081 tttatcaaag aaaaggcaaa tagattgtac tgactaaatt cacgaggatc agtgatgaga
    70141 gcttattgct aagagcagtt aggctctttt ttatttgtgt aaaattattt tattttacaa
    70201 tacacattta tacacaaaaa atgttgacgg atagtacaca aatgtggtag gatatatttg
    70261 taagcaagtt agattaacaa gttaaaggaa ggaagagcag ttatgaaaaa agaagaacta
    70321 atagatatgt ttcaaattgt tgaacgagca aataatatgg gaattatgtt ttttgatcgt
    70381 atttctttaa agatggattt aagcgttgcc caccaagaat ttaatctacg attaaaagct
    70441 ttattgattt ctgatgatgt taatttcgct catgatgttg taggtataca gaaccatata
    70501 gatagagaaa acaaacgtat gggtgatgga ttcttaccaa gatattcaag tttatgattt
    70561 ggcgaaagat tcaagagtat gtgtcattat tgataggtac tcttgaattg gaaaggagga
    70621 atgaatttaa gacaacaacg attatgatct taaaacaatc gtttatgtaa ttggcaatct
    70681 tacgaataat tttcatgcaa taatgacaat aaaaaatgaa aaggggtaaa atcaaatgaa
    70741 aaaattttta gtaggaattc tattcacaat ggggctagtg ctattatcac cttcagtttc
    70801 agcagaaagc tctatttcca tgtatcgtat gtataatccc aatagcggag agcatctata
    70861 cactagaagc gttggagaaa gagacaatct gaaacgttat ggatggcatt acgaaggtgt
    70921 cgcgtggcaa gctccaacaa gtggttcccc agtttatcgc ttgtacaaca gatataacgg
    70981 tgaacatttt tacaccgttg atgccggaga aagagataag atttcgcaca caggatggac
    71041 gtacgaaggt gttgcgttct attcctacaa cggagcaaat agagttcctg tgacacgctt
    71101 gtacaacaaa tacgtgaatt ggcaccatta cacgttagat caaaatgaaa agaacgtaat
    71161 tagtaaacaa ggttggaaga ttgaaggtac gggttggtat gcaatgccaa acagcgcacc
    71221 aaatcctaca cctgcacctg ctccaagtaa tgaaaaagtg ttaaatgttc ctgtcatttg
    71281 gcaaggtaat acgatgttgt gtgaaggtgc ttccctgtta gagggattgc actacaaagg
    71341 agtagcgaca aatcaaaact taaccagttt tgtaaactat atgccaagag ctaatgacaa
    71401 taatccatat catggctatt ctggcgaatg gcgctacaac gtaaatggta cgtatcaagg
    71461 catgatgcct gatccagtag ttaaatgggc aaaaaatgtt ggtggtaatg ccgttaatat
    71521 cactggttca ggaacgaatg ggatcaagaa tgaaattcgc aatggtaatc cggttgtaac
    71581 ttggattact tataactatg cttcacctca attcaaacaa atgccttggg ggcgtgcagt
    71641 ctggaatgga catgtggttc tcgtggatgg cttcaaaaat ggtgcatacc atgttgttga
    71701 ccctgtattt ggcgcaaaat ggatcaatgc aagcacgtat gagagagcat ttaatgtgac
    71761 tggaatggcg gttgctgtac gttaattgat agaattaata aataaatgaa ataagagctt
    71821 aattactgaa atggtaaatg ggctcttatt ttttagaaaa aaactgtttt tttgtaatac
    71881 gaatctataa caaaaaaacg ttgaatgtag tacacaaaag tggtatatta aaaccgtaga
    71941 gattatgcga agaggtgatt ttgatgaacc aaagaagaag aaaaaggcta gaagaaataa
    72001 gcgagcgact atccgagtta aaggatgagt tagaagtaat tcaagaagaa gaggaagagt
    72061 acaaagaaaa tatacctgaa aatctacagg gatcagaaag atatgaaaaa gcagaagaat
    72121 ccgttgatca acttgaagaa gctgttgatg gtttatccga catattagaa ttgattgaag
    72181 atatagctag ctagagatga ttaatgaaga tttcaggagt gagcaaaaaa tgattgatat
    72241 ttcactgata gagaagctac ttttttcaaa taagtacaca gccaaagagt tggctacaag
    72301 atggcaaata tccgaatcag ctattaaagc ctatcgtcaa aataagggaa gcgtaggatg
    72361 gcgtgattgg aaaggaatca gtattggaaa agcagaagac ataatgaaac gtgaaggatt
    72421 tcaagctgac aatgattcta aaaaagttga atagtttaat agtcgccaag aaaagtacca
    72481 ggacgagggg aaaggtagat tacaaaggaa aacactttta aataaacttc gcaaaagcat
    72541 caggttatga gtggctaggg ctatacgaat gtgatgaaaa caaagggctt taatgtcaaa
    72601 gaaaacgcaa taggggggga ataaaatttt taaaaaagat atttatgttt ggtttaatgg
    72661 aaaaaaatat atgactacta acaaaaggtg gtcagagcta gtacaaaata cagttaaaaa
    72721 agtcatgcca gatggatatc atttcaacta tatacaatgc tcacaaacaa ctgaaagtat
    72781 ttatttttca atattaaatg agaatagact ctattatttc agggtaagtt cacaccatag
    72841 gaaaaagaga gaagataatg ttacgacatt tttatcggga aaatacaatg gcttcaaaga
    72901 gttaagtgaa gcaattggtg agtatctaaa aagtaataga tttgtcagaa tttctgacaa
    72961 acatatattg atcttgttac tttatttgaa taatagcttc aaattatttt ggagagacga
    73021 cactttctac tatattgaga acggcatgga tatttattct ggtatcaacg gcatattaaa
    73081 acattatatt aaggcgttac ttcgatatgg ggttctttat caagaaaatt taagtaacca
    73141 aattaagatt agtaattctg gaaagtcatt actggcaaaa tataaaaaat tgctttctcc
    73201 tatttacaaa ggaatagata tggaagctgt aaattctgaa actttattta atttagttgt
    73261 tgatgaaaag tttgcgaatg aattttttaa aagtgaaaaa attgaaagac taaataacct
    73321 gtttcctgaa aatgcaataa aagcttatgt agataataat aaatcacgat tatacaactg
    73381 gtttattccc aaacggttgc gaggaaaaat caaaattggc gatcaagtga aagtaattaa
    73441 tctaaatcat actaagatcg ttaccgtaac ggaattgtat atagctgaaa aagaagagat
    73501 tacaaaaatg aaaccagtta ttaaaatact agataacaaa gagtgagtgg aatatatggt
    73561 agcaaaaaag aatactctaa aagaagcttc tgaaaaatat caagatatga aaagacgagg
    73621 attaatacca aaggacaaaa aaggtatatg gagagaattt gttaaggaag tcagtagtga
    73681 tcgaagtggc ggtgactttg ttggacgata tacagcaaaa ggttatagca ggcgcttaag
    73741 gatcactgtt gaatatggaa cgaatgaatc agctacagag ctgaagcaaa ggtacttaga
    73801 tacacgaaga gaagcttcaa aaaaatatga tgatttcaag agcagaggtc taattcctaa
    73861 gaagaaaaag gggatttggc gtgagttttt caaagaagaa gatagcaatc ggaataacgg
    73921 agacttcatt ggacgataca cagcaaaagg ctacactaag aatttaggga tcaaggttga
    73981 atacggaact ggtgaaagtt catgggaatt gagaaatcgc tatttaaata cattgttaga
    74041 gaaatataaa gcagaattat tggagttgtt cgatgttaat gattttatcg agatggctga
    74101 aaagtatggt catgaagaag gttttatgat caatgacatt atggttattg ataattttac
    74161 ggatttatta gcacttgcga aaaaagcaag ggatttaaga gaaacgcaga tgtatctatt
    74221 cagaaataaa aacggaagaa accggagaac tgaatcgagt gagattgaag attactacga
    74281 tgcctttaca cacgatctgg gagcctttgt agacgagata agtggtgtaa tgaacaaata
    74341 tagggtctag aataaaaaag ccggagatga gcagataaat gggaaacaat agcatataca
    74401 cacctgaaat gcagaataaa attaaaagac ttgtcggatt agggtggatg acaagaggaa
    74461 aattagattc ttttagtacg atcaaatatc ttttgatgga catttaccgt cagaacaagc
    74521 aaacaggaat acattttttg ggctatgata agccggaaga tgcacctttc tttgttccaa
    74581 gttcagccta tagagccagt acctccggta gcatgtataa cttggatttg ttcgaggaag
    74641 gagcaaagat gatcgaatat gatttcaacg aagcttattc gaggatcatg agaaattatc
    74701 ctttgccgtc aaacacttat ttgaaagtaa aatataatac agatcaagct attttgagag
    74761 cgataaatag accaaaagta agaccgtatg aaaatttaaa aacgtatatg ttctttttgg
    74821 ttaattttga aggaacagcc aaagaaggaa catatgctaa tccaaaatca cagtcgatgt
    74881 ttgcggatta tggcgaaaat cccaaaacag ttaatgagcc tatttggtta tcggagattg
    74941 agctaacact tatccttgat tattatgata ttaaagagat ggaagtccta gatagccaca
    75001 catttttgac gaaaaaagga ttactcaaag aatattttga aagaatcgag ccattaaaaa
    75061 ctgatcctga tacgttgaag ctttataaaa atcttagaac gaaagtattt ggtgctatcg
    75121 gacaaaaatc attaagagaa ggagaaacaa gcaccttcga taaatacagt atgtataatc
    75181 gagcgtattc ctccatggta gcaggcgtat ttagagatcg tatggttaga tatgaacaga
    75241 aatacgttca tagcgcttat gggctattaa aaattcgtac tgatggattg tatttcagat
    75301 ccgaagtacc agagtttgaa gagctgtata aacttggagt agttaagaaa aaagttcata
    75361 caatatccaa agaagaagtc gaaaaagctt tagaatttaa aaataaagga tttgaaaata
    75421 agggctcctt tgcgaaatct aaagatgaag ttaagggtgt tgtgatagac gatgatttcg
    75481 agtttgattt tgactaagta ctctttggaa aagatggtag gaggtaaata tgaagaagaa
    75541 aattgtggaa ggaaatgtag gtgttattat tctaaaagat tcaactgact atgctaaatc
    75601 aatggcacta aaagacgaaa aagttgcacg catggataaa gatttagttg aatttctgat
    75661 ggatgataca gaaagattaa aaggggatca gtttttgagt ttaatgaaag acaaaggctt
    75721 aaacacatat ggtttatttg attataagaa attagctata gagtgggtaa aagaaggaga
    75781 aatatttttt attgaagaat gggaaggtac tgagaactta gttcttcatc agtatctttt
    75841 gaaagcttaa tctaagtaaa acagttctga ctagttatgc ttgtcagaat ttcaaaggat
    75901 gttttccata accatcatta ttcaaaaata aaggatttga aaataagggt tcctttggga
    75961 aatctaaaga tgaagttaag agttttgtga tagacgatga tttcaagttt gattttgact
    76021 agatttcttt ttgcaacgtt taagagtaaa caaggtaaga attaagattg tgaagattga
    76081 gagtaaagaa aatagaggac gaatgtatga gaacacacta tatttattta gaagataaaa
    76141 ttgtagatga aattccggca aaaagtcgag aacaagctag aaaattattt attgaaaaat
    76201 acaaagagat caatggagat agtgaagttc cgaaagtggt cgtaacagtt agaaaaaggc
    76261 aaacaaaaaa gagaagtacg tttcatgtca gccctgatat atataatctt atagagcgac
    76321 atagaagtga agataatatt tctatctctc aatatattgc tacagttatt gatgaattga
    76381 tttcaggaaa gtatgactat gcttttgatt ttgaggatga aaaggattac aaaagtgttg
    76441 ctgtctttca atctgatttt gagaagctaa aaaactatgc tggcaggaga aatgttaatt
    76501 ttgcaagact actagaagag ttctactccg aagagttggg agtaggaaat gaatacaaga
    76561 gagaaactta tgcgaaattg tctcataagt tatcccaagc gcttaaagaa gtgacagaag
    76621 ttaaagcgga actagatgct aaacttgaag aagtgaaaaa atatcaaaca gagaggaaga
    76681 acaacatgaa gaatcaagtg gaatttgacg aatagaaaga atttgaaaaa gcaagaaaac
    76741 gctataacaa aattatgaat gaacttagct atgaacactt aacagttcat gaataattga
    76801 gtgagggtac agaaaaatat acgatcgcag atatggttaa tgaagtggaa actcttttag
    76861 cttctttctt tgaaaatggt cacgtaaacg atgaatggcg aaaagaagaa cctaaaaggg
    76921 ttaacgctta tattgctcga ttaaaaaggt ttattgataa atataaggag aatagtgaca
    76981 aattttctaa agataaataa ggagagagtg tgattacatt tgccagaaat cgaaggaata
    77041 ttttcgccaa aaataggtaa aaaacagtta gatttcagca gttttccgag cgtaaataga
    77101 gattataatc taggtgatga ggtacagata ctaaatattg atttcaaaga actagacttc
    77161 gctgttgctg tttccaacaa acccaaggca ttggaacgag tgaaaaaaat ataccaagag
    77221 aatgatcaaa atatcaaaaa tatgatcaaa aagtatagac gagataattc aaaagttgaa
    77281 gacgttttat tcaacggtac ggcaaaacat gatttgaatg tttatatggc attaggagtg
    77341 gctttgtact gtaattcaat taatcagaca gatattttgg ttaatatcgt gaggaatagc
    77401 tatcaaaaaa tattaaaaat aagtagcgaa acaaaccctt attatgcaat gaaatatctt
    77461 ggggataatt tattaaatga taaaaaattg atgcacaaca ctgaagagct gttatctaat
    77521 ttggctgcga ttaactttct attcaaaaca ggttctgaag attattttat agataacgta
    77581 ctaaaaggtg cttacggaac agcattttat ccgctttaca atatgaaaca gcataaagcc
    77641 gaacaaaagg ataacaaact tgaaattaaa aagatgaaag aactattagg tataggattt
    77701 gtaaaaccca attcaaaagt cactattgct tcgttagcaa ctactaacaa tgatgggaaa
    77761 atagtaaatg ggtttgacga ttcagatata gatatattgt caaaatcata tttttctttg
    77821 tatggagatt ttgcagattt gatgggtatt gatagttact tagtcaataa ttttgagatc
    77881 aagggtagcg agctgaatga tattgtgtat ttcgttttaa tctctctata tgcaattgat
    77941 cagaataccg agtaccgaca aattgactat ttgctagttt tttatagttt gttatatcag
    78001 ttcgttttaa ctcggttgtt taaaaatgct aaggaagaat tgatcctgaa ttctgattat
    78061 gaaatagcaa aacaaattgc aagtattaga aaagaaaaca aatctgaagt aatcaaatta
    78121 agaaaagaat taagtattgc gagtaaagaa aacggtcggt tagaatcttt agtcaaatcg
    78181 gcaaaaaaag aagaggaact tttgaagcaa gagattaaag aattaaaagg aatcagaaaa
    78241 gaacttgaac agctgaggtt tgaaaataaa gtattaaagc aattgcagga gcaagaacat
    78301 agacaatcat taaatggtaa aactgaagag ccttcactgt ctgaaatgat agcttatcta
    78361 aaacataaaa acattctttt agtgggagga catgtaaatt ttcaaaataa attaactgaa
    78421 ctattaccaa atattaaaat gtttgatgta agcgaaaata atggaaagat acttaaaaat
    78481 ttgaatacgt gtgatgcgat ttttctatat acagattact tgaatcatgg tctatattat
    78541 cgtgttatga gtgaaatttc taataaagat aagatatttt atttggataa tcatgtgaat
    78601 atgaacttaa caattaaacg tatttacgat cttttatcac ttatataaag agagaagtga
    78661 taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta actttatctt
    78721 ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct gattgtataa
    78781 tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa atttcttata
    78841 tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc gataagcttt
    78901 tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt aatgtgatat
    78961 tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt ttcatagttg
    79021 aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatatat gcattttatt
    79081 taataggttc aacaatttac tttaataact tgtatacacg tattcaaata aaaaagcagt
    79141 tattacatga aggattaagt ggtaatatgt taaaaagaat tgcatgggat aaagataata
    79201 aaacagatat tgagcttaaa gaatataaat acaattattt ctcgagatgt atagatagaa
    79261 agttactgtt taaaaaagaa ggattagaat ttagttttga tgaagatgaa tttgaaaaat
    79321 tagtgatgga ctggcgtaag aatttactgg atacggtaca attctaaaag caattgaggt
    79381 cgaaattccg atacttgaat catgatctat attatcgtgt tatgagtgaa atttctaata
    79441 aagataagat atttcatttg gacagtcatg tgaatatgag cttaacaatt aaacgtattt
    79501 atgatctttt atatgtttat aaacattaat gaggtgctaa aattgacaac aataactatg
    79561 acaaaaaaaa tagaagtata tgttgatgga tcacaagaag aaagggattc cttttttgat
    79621 tttttttgga aaaatagtag agaatatgta aaagcgttga atctggtata taaacagcta
    79681 atgtttgaag agttgataaa agatgcaatt aaagaaatgg atcaaggtta tgctgagaga
    79741 gaagaaagta ttcaaaatcg gattgaaaag ttagcatata ctgatccaat agatcaaaaa
    79801 aaacttgata aagcaaaagc tgaattatct aaattgagaa atgaaaaaaa taaagaagcg
    79861 agagaaatat taacgcaagc gatagggttg aagagtcaaa cgagaacacg agatgtagca
    79921 aaaaaattag actttgactt tggagatttt gttgattccg caaatatgaa agctagtaaa
    79981 gattttggca atgattttat cggaattgtg accggacaaa gaacgcctag aatatataaa
    80041 aatagaaaca aatatggatt attacctttt agaggtcgag atattaaaat ccataaagat
    80101 gataagtttt atatttattt acctaaaggc tttaggttta aagtgaattt aggtagcaag
    80161 ccaagaaaag caagagatgt tgcttcagtt ttacaacaca ttatggatgg cgattacaaa
    80221 ttaaatcaaa gtaatcttat ttttgatgga agaaaattat tttttaatct aacaatttct
    80281 tttgaaaaaa gctataatga aatggaattc aagaaaggta atgaattgat cgtaaacttt
    80341 gcagatttgt gtagcggtga atgtttaatt aatgataaat ctgtaatgtc ttttggtgat
    80401 agccaatata taagaaactt caaaagtaaa attttagcaa tgaaatcaaa agaacaaagc
    80461 cgaggagtct tttctaaggg cggtcatggt cgaaaaagaa aattagatca agatcgatgg
    80521 gaagcaataa agaaaagaga aagtaatttt gttaaaactt ataataatca atgttctgca
    80581 aaaatcgtta aaactgctat tcgcaataaa gctcaaatta ttcgtatcat tgaaccagat
    80641 aaggcttatg gcgaatgggc ttactatcaa ttgaaagaac aactagtaaa caaaggaaaa
    80701 agatatggga tagaagtcag aagttagtaa attaaacaat cgttgatttg gcaacgaaat
    80761 agtgagggaa aatccactca ctgaagggta aactccgtca agttgtgtga ccagacggaa
    80821 ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atgtttctca aatttttagg
    80881 ataagggaaa ttgctagcag tttgttttag aaccatataa aacagcatgt ttctcaaatg
    80941 agtactttaa attaattgat ggcgttcctg ctttagaacc atataaaaca acatggattt
    81001 aaaacaagaa gtaagtcagt tctcctttta ctttagtaag gaaaaataaa gtattctcaa
    81061 taaaacttaa aataagcaaa cctttgtttt aatagctaat cgaagtaaaa cccaaagcca
    81121 tatgaacccc tgaatcagcg aaagtttatg atgatttgga ttactaatag tttaggaatt
    81181 agatggtaaa tctaaattaa acaatcgttg atttggcaac gaaatagtga gggaaaatcc
    81241 actcactgaa gggtaaactc cgtcaagtta tgtgaccaga cggaaggacg aatgttgttg
    81301 aggttttaga accatataaa acagcatagt tctcaaattg tttaaaatga ttgtcaagtt
    81361 aaaaaaatct gggatcatat aaaatagtat agttctcaaa tatgattaga aaccctatta
    81421 aagagcaata ttaagccaac ttacttaaat cgtttaatta agaacaataa tctcttttag
    81481 tcagactttt agtctgactt ttttgtattt ccaaaattta ttctttacaa aaacgaacat
    81541 acgtttgtac aattacttgt gaatggggga ataaaaatgt ttgattacag tagagaaccg
    81601 gtgaatgact attttttaat cgacatgaag tctttctatg ctagcgtgga atgtattgaa
    81661 cgaaatttag acccaatgac tacaccgtta attgtcatga gtagatcgga caacactggt
    81721 tcaggtctga ctctagcttc ttcacctact gcaaagaaaa aatatcatat tactaatgtt
    81781 agtagagcta ctgatttacc ttatccatta ccaaaagaac ttcatattgt tccaccacga
    81841 atgaacttat atataaaaaa aaatatggaa gttaatcaga tttttagaaa gtatgtcgca
    81901 gatgaagatt tattgattta ttcgattgat gaatcaattt taaaagtgac aaaatcctta
    81961 aatttattta ctagttcagg aacaagaagc gagagaagaa gagaattagc taaaataatc
    82021 aaagatgaga ttcagaaaga aacaggtcta atagcaacag tcggaatagg agataaccca
    82081 ttattagcta aacttgcact ggacaatgaa gcgaagagta gtaaagaatt tatagctgag
    82141 tggacttatg agactgtacc tgaaaaaata tggaaaataa acgagatgac tgacttttgg
    82201 ggcattggat cacgcatgaa aaaaagattg aatcatatgg gaattatgag tatagaagat
    82261 ttagcaaatt ggaatcccta cacaattaaa agtcagttag gtgtaatcgg actccagtta
    82321 tatttccata ccaatggaat tgatcgtacg gatattgcta ttccaccaga accaaccaaa
    82381 gaaaagtctt atgggaattc acaagttcta ccaaaagatt atacgaggag agaagaagta
    82441 gaactagtag tcaaggagat ggcagagcaa gtagccattc gaattagaca acacaattgc
    82501 aaaactggtt gtgtacgctt agcaattggt acgtcaattt tggaagaaaa atcaggtttc
    82561 tcgcaccaga tgaaaatacc aattacagat aatacgaaag aattacaaaa ttattgtctt
    82621 tttttattca ataagtttta tgaggggcag gaagtacgac atataggagt aacttatagc
    82681 agattagtat acacagattc cctacaatta gatttattta gtgatccaag aaaaaagatt
    82741 gctgaagaaa atttggatag gattattgat aagattagac aaaaatatgg atttacagct
    82801 attatacatg cttcatctat gttagaaggt gcaagaagta ttacgagaag cactttaata
    82861 ggaggacacg caggaggatt aggaggtttg cataatgatt gaatataacg atagaggacg
    82921 tttaaaatgg tcgggatttt atttgtctga tcatactgaa agaatcaatg cagacaatgt
    82981 acaacgtgct aatcataatt tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat
    83041 actgagaact gctgtcataa aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc
    83101 tgaaggttac tatccagatg atgtagtagg aaaaattatt ggatatgacg agcttggtgt
    83161 atttgtggaa gaagaaaaag ttcactatga tgaaattagg aatgtttctt tctcggaatt
    83221 attaaaatgg agtcagatat gaacattatt aaaaaatacg aattcggtta tattacgtat
    83281 gaacagctaa tagaggaatt atggggatat ggtcaacaat taattaatga agtggggatt
    83341 gattgctttt gtttctatat tgaatccgga gcaggatacc ataagaacag atactatata
    83401 tttccatatc caccaaaata gtctcttaga tatttttaaa ttttctccaa atactttcaa
    83461 ttattgtgaa atatttccct ttaattaatc aaatttttta taaaagtaat atataaaata
    83521 aattgcttga agtattatat ttagatatca taatttatgg aagaaaaatt aattaaaaag
    83581 gatgagatta taatggggtt actggttccg agagctaata cttatactat acctttaacg
    83641 aaacgccaat taattgctaa aagatatcaa aggattacta gagccatcaa tagagagttt
    83701 tggaattctg aaagtgatac tgctcacagt ttatacgtag gttcttatgg gagaggaacg
    83761 gcaattagta ctagtgatat agatataata gttgaattac cgatggcaga atttgacaga
    83821 ttcaaaaatt atttatcaaa tggcccgtct aagttattac aagttataaa aaacgcattt
    83881 caggaaatac taccaaatag tgatattaga gctgatggac aagttgttaa aataaatttc
    83941 catgatggaa tcaaatttga aatagttcca gcttttaatg aaaaagatta ttggggtgag
    84001 tcaaaagggt ttatttatcc agattcaaac atgggaggga actggaaagc tacaaatccc
    84061 aaaaaagagc aagaagccat gaaattaaag aatacaaaaa gtaataattt attatatgct
    84121 acgtgtaaac attttcgaca tgtacgagat acagaattta cgagttatca tctttcagga
    84181 atagtaattg acagtttcgt atatgaggct atgggaaatt ggaaattcgt tgaaaataac
    84241 agtgggggtc aaaacattag ttctgtatct tatgagacag ctttattaga atactataac
    84301 tcacataaag ttatgggcgg tttaaattta tactctcctg gaagcaatca atttgttaat
    84361 tctgatagta gtatcatatg tttagaaaaa gtattaaaaa aaatagcttt ataaagtttt
    84421 atggaggaac aacatgggag acgaaaaaaa gcgcttggaa gttcaattac gtgaagcata
    84481 tggacgagta acgtatactt acaccactca tttaaagcaa gccgataata ttatttctga
    84541 caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc cttactactg gaggggtact
    84601 tagtaccatt attactgata atagtgagtt aacctttgtt gctggattat gttcaacaat
    84661 ttctcttgga ttaaacttat actttaagaa ttttgatctt gctgaagaag ctaaaaatca
    84721 tcaaattgtt gccgataaat tatggttgat cagagaagaa tacgtaagct tactaacaga
    84781 tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga gatcaattaa tgaacgaaac
    84841 tcatgaagca tataaaaatg cccctaaaac aaatagtaaa agctatgaat cagctcagaa
    84901 agcattaaaa tctgaagaag agcaattttt tacagaagag gaattggata agatgttacc
    84961 agagcatctt agaaaaaaga gttctaaata ttgaactctt tttttatgct taaaattctc
    85021 ttgtaatttt tctattcatc tatatttaag aagaaatatg atgatagaaa catctcctaa
    85081 agccaccgca agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca
    85141 ccatgaacgt ttcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc
    85201 tagatatgct catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagccatt
    85261 gcctagtccg acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc
    85321 aagccgttct aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg
    85381 tttcctaaag ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt
    85441 tccatttctg tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa
    85501 gattactagg tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc
    85561 aagctacgcc tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt
    85621 ccttacgccc attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct
    85681 catctttgga ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata
    85741 atttacttaa acacttgtat taatccggca gataagaacg cattctaatc actcccgaca
    85801 aggcaagaca ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa
    85861 ccaaatagcg atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt
    85921 tgaaaaacca aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa
    85981 gttaatcaag aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat
    86041 ggtactttac ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt
    86101 aaaccgagag cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat
    86161 gaaatggtta tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt
    86221 tctgatatgg gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa
    86281 ggtgaaggat tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga
    86341 agtttttata gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga
    86401 aacaatgcta gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct
    86461 tatagcgaag ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt
    86521 caactagctt tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga
    86581 tatgaatttc caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa
    86641 acaattggta ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac
    86701 tcatttcaag aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggtga
    86761 aagaacacaa gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc
    86821 aattcattat cagtggggat atggtcgata catgttgatg gacgttttga atcaaggaat
    86881 atatttaatc aataaagcat atgacttacg agatgaatca agagaagaat tagcaaaagg
    86941 aatctttgat agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc
    87001 ttatgatgaa acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt
    87061 gatgaagttt tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa
    87121 tggatttagt ctagatgata aaaccagatt gcgattgttt gattttgatg gtcaacagat
    87181 aggactaaaa aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg
    87241 atacagatta ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt
    87301 gtttgaaaaa agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga
    87361 tggtgaaatg attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg
    87421 taaatactac tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga
    87481 attggcaaag aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac
    87541 cgttagaaca acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca
    87601 tgaatttgta gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca
    87661 gagttgccac gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga
    87721 ttatgagatg gaaaaagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa
    87781 gagaagcgat gatgaaattg aagattggtg tgtgtggtta gtaggggcag atgattgcgt
    87841 tgcatattac acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttcta
    87901 gatcatgaag atttggttga ttttggttat acatggaaag gaatggtagg tattagcaga
    87961 agtcttgcta atgcctttta cgaaagaaat tatgcagtgt atgttcttta tgatgacgat
    88021 acagaaagcc ttgttgacga ggaatataaa ttggatttag agaatgtttt atatggaata
    88081 gaaaaggaag atttagcaaa atatattttt tcatggttgg gtcaataagt gaaaggaaga
    88141 tagaaacatg acaaaattta aaagtaaggg tatgcaatgg tgttcttatt attatgttgg
    88201 gaatgatgaa cagcttttga gaaaagccca acaaaaaggt tatccagata agatgttaaa
    88261 acagattggc tattacacgc cagaagagag gatagaaaaa gaatcttgtg aggtactttt
    88321 tgatagtgaa cagctattag ttttgttaac tcaaaataaa ttaatttgtc aatttgagaa
    88381 taattaatat ggggtgaata accatgaatg agaataagat ggtattaagt gaatacgcaa
    88441 gattgagaac cattaattgg ttgaatggta tgcaaagtag tggcgctttt gatggatttg
    88501 atgaagagaa tatggacttt gaaagtttat cgaatgagga tattagatat tattttatcg
    88561 aatggtattt tacagaacca gatgaagagg tggaagatga acaagaatta attttagaac
    88621 gtcaaagtat tttatgcagt gaacaagata gtaagaggtt gtatggttta tatgacttgc
    88681 aactgaatgg attaaaagcg cagtacgttg ctgatagttt cgatgatatg gtaggacagg
    88741 gggcagatag ggttgtcact tattcagaag atgatgaaga ggaatgggaa aactattcag
    88801 atgatgagat atgctttaat gatgggtata tgcctattga aatcaccgaa gaagaatatg
    88861 ataaagtgtc cgatagtgat gagattggta tgtgtacggt tgtaagtgga ttggatatag
    88921 atgtccaaag agtagaggtt gcataccaga agatgaaaga acgtataaaa agtatagtag
    88981 aatcttgatt taattggatt gtttgcaaag tatctaatta agagtataat ataatcataa
    89041 atgtagttaa tggatacaat aatgagtata ttatacttaa aagaggtgaa cgaaatgaag
    89101 gtaaaaaatc caactgaaaa aagcagaatc caagtcggaa tagacaagaa tttaaaagag
    89161 aacgcagaaa tgatattgga agagctaggt ttaaatccta caacagcaat cacaatcctt
    89221 tataagcaag tggtcgctag aggtgagttt ccagtagaga taaagctaag tgaagaggaa
    89281 aagcaaggaa tcagattgca acagctaact aaggatatgc cagttgatgt tctagataca
    89341 gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag atagtaacgg tctatgttgc
    89401 atttacagat aaaaatggtg gtaaaagaag acctattttg gtggttagtg ataaagaaga
    89461 tagagtagaa ttttttggaa tcacaagtca atatgagaag aaatcagacc aaataaagag
    89521 agtgtatttt cctattgcag aatgggaaaa agcaggactt aaaaagcaat catgggttga
    89581 tgtagggtct ttaaaggcta ttcctaaaag ccgagagaac atatcattta aaaaggttgg
    89641 tatgctatca gctagtgata cgaaaaagct aaataggttt atcgagagat tacaaagaaa
    89701 agtctaacaa ggcttttctt tttttgatta tatgtatatt aagatatacg tttgtataaa
    89761 tgtatttatg tatataaaaa taaacgtaaa tatatacgtg attataaata ttaagaaaac
    89821 attaagaatt gtttaatagt aataattagt aatatacgaa ttataagatg taatttatat
    89881 tgaatgtatg taaataaata aaatggctga cggatattga atagcaataa ataacaaaaa
    89941 gccagacgga tacagtgagt gaagtagctt gctacgaacg aacagacaga atgaaacggc
    90001 agtttcatta gagcaagtcg cttgcgacgt agcgttaccg ctgatgtaac tggtaagtta
    90061 catagagaga gtttgcttgc aaacgaacgt tacctacttg aatagaaata aaaaaataac
    90121 atattagtga attttgaaca ggagccacaa ctcacccaac cgcttgcggt gggtgagtcc
    90181 cacgga
//
LOCUS       DACCQP010000004        83467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCQP010000004 DACCQP010000000
VERSION     DACCQP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.004"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..459
                     /locus_tag="IVO87_000317"
     CDS             <1..459
                     /locus_tag="IVO87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7448546.1"
                     /translation="IAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQA
                     EQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNG
                     RYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(543..1265)
                     /locus_tag="IVO87_000318"
     CDS             complement(543..1265)
                     /locus_tag="IVO87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7448547.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1255..1992)
                     /locus_tag="IVO87_000319"
     CDS             complement(1255..1992)
                     /locus_tag="IVO87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448548.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(2004..2996)
                     /locus_tag="IVO87_000320"
     CDS             complement(2004..2996)
                     /locus_tag="IVO87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7448549.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(3011..3754)
                     /locus_tag="IVO87_000321"
     CDS             complement(3011..3754)
                     /locus_tag="IVO87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448550.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3828..3932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3995..5317)
                     /locus_tag="IVO87_000322"
     CDS             complement(3995..5317)
                     /locus_tag="IVO87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7448551.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5304..6269)
                     /locus_tag="IVO87_000323"
     CDS             complement(5304..6269)
                     /locus_tag="IVO87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448552.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6266..7804)
                     /locus_tag="IVO87_000324"
     CDS             complement(6266..7804)
                     /locus_tag="IVO87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7448553.1"
                     /translation="MNSFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8220..8507)
                     /locus_tag="IVO87_000325"
     CDS             complement(8220..8507)
                     /locus_tag="IVO87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448554.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8641..9309
                     /locus_tag="IVO87_000326"
     CDS             8641..9309
                     /locus_tag="IVO87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7448555.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9600..10340)
                     /locus_tag="IVO87_000327"
     CDS             complement(9600..10340)
                     /locus_tag="IVO87_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448556.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10470..11417)
                     /locus_tag="IVO87_000328"
     CDS             complement(10470..11417)
                     /locus_tag="IVO87_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7448557.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11546..12310)
                     /locus_tag="IVO87_000329"
     CDS             complement(11546..12310)
                     /locus_tag="IVO87_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7448558.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12314..12703)
                     /locus_tag="IVO87_000330"
     CDS             complement(12314..12703)
                     /locus_tag="IVO87_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448559.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12838..13380
                     /locus_tag="IVO87_000331"
     CDS             12838..13380
                     /locus_tag="IVO87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7448560.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13528..14160
                     /locus_tag="IVO87_000332"
     CDS             13528..14160
                     /locus_tag="IVO87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448561.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14294..14419)
                     /locus_tag="IVO87_000333"
     CDS             complement(14294..14419)
                     /locus_tag="IVO87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7448562.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14553..15446)
                     /locus_tag="IVO87_000334"
     CDS             complement(14553..15446)
                     /locus_tag="IVO87_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7448563.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15628..16554)
                     /locus_tag="IVO87_000335"
     CDS             complement(15628..16554)
                     /locus_tag="IVO87_000335"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7448564.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16639..17400)
                     /gene="pflA"
                     /locus_tag="IVO87_000336"
     CDS             complement(16639..17400)
                     /gene="pflA"
                     /locus_tag="IVO87_000336"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7448565.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17644..19875)
                     /gene="pflB"
                     /locus_tag="IVO87_000337"
     CDS             complement(17644..19875)
                     /gene="pflB"
                     /locus_tag="IVO87_000337"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7448566.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20152..22602)
                     /gene="parC"
                     /locus_tag="IVO87_000338"
     CDS             complement(20152..22602)
                     /gene="parC"
                     /locus_tag="IVO87_000338"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7448567.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22614..24659)
                     /gene="parE"
                     /locus_tag="IVO87_000339"
     CDS             complement(22614..24659)
                     /gene="parE"
                     /locus_tag="IVO87_000339"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7448568.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24825..25259)
                     /locus_tag="IVO87_000340"
     CDS             complement(24825..25259)
                     /locus_tag="IVO87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7448569.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25386..26024
                     /gene="plsY"
                     /locus_tag="IVO87_000341"
     CDS             25386..26024
                     /gene="plsY"
                     /locus_tag="IVO87_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7448570.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26156..27031)
                     /locus_tag="IVO87_000342"
     CDS             complement(26156..27031)
                     /locus_tag="IVO87_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7448571.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27113..27904)
                     /gene="codY"
                     /locus_tag="IVO87_000343"
     CDS             complement(27113..27904)
                     /gene="codY"
                     /locus_tag="IVO87_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7448572.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27922..29322)
                     /gene="hslU"
                     /locus_tag="IVO87_000344"
     CDS             complement(27922..29322)
                     /gene="hslU"
                     /locus_tag="IVO87_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7448573.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29336..29884)
                     /gene="hslV"
                     /locus_tag="IVO87_000345"
     CDS             complement(29336..29884)
                     /gene="hslV"
                     /locus_tag="IVO87_000345"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7448574.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29921..30826)
                     /gene="xerC"
                     /locus_tag="IVO87_000346"
     CDS             complement(29921..30826)
                     /gene="xerC"
                     /locus_tag="IVO87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7448575.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(31024..32400)
                     /gene="trmFO"
                     /locus_tag="IVO87_000347"
     CDS             complement(31024..32400)
                     /gene="trmFO"
                     /locus_tag="IVO87_000347"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7448576.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32369..34447)
                     /gene="topA"
                     /locus_tag="IVO87_000348"
     CDS             complement(32369..34447)
                     /gene="topA"
                     /locus_tag="IVO87_000348"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7448577.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34569..35426)
                     /gene="dprA"
                     /locus_tag="IVO87_000349"
     CDS             complement(34569..35426)
                     /gene="dprA"
                     /locus_tag="IVO87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7448578.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35482..36249)
                     /locus_tag="IVO87_000350"
     CDS             complement(35482..36249)
                     /locus_tag="IVO87_000350"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7448579.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36242..37102)
                     /gene="ylqF"
                     /locus_tag="IVO87_000351"
     CDS             complement(36242..37102)
                     /gene="ylqF"
                     /locus_tag="IVO87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7448580.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37362..37760
                     /locus_tag="IVO87_000352"
     CDS             37362..37760
                     /locus_tag="IVO87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7448581.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37997..38539
                     /locus_tag="IVO87_000353"
     CDS             37997..38539
                     /locus_tag="IVO87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7448582.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38622..39173)
                     /gene="lepB"
                     /locus_tag="IVO87_000354"
     CDS             complement(38622..39173)
                     /gene="lepB"
                     /locus_tag="IVO87_000354"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7448583.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39325..39552)
                     /locus_tag="IVO87_000355"
     CDS             complement(39325..39552)
                     /locus_tag="IVO87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7448584.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39549..40067)
                     /gene="msrA"
                     /locus_tag="IVO87_000356"
     CDS             complement(39549..40067)
                     /gene="msrA"
                     /locus_tag="IVO87_000356"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7448585.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40087..40800)
                     /locus_tag="IVO87_000357"
     CDS             complement(40087..40800)
                     /locus_tag="IVO87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7448586.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40803..41699)
                     /locus_tag="IVO87_000358"
     CDS             complement(40803..41699)
                     /locus_tag="IVO87_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7448587.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41857..42699)
                     /locus_tag="IVO87_000359"
     CDS             complement(41857..42699)
                     /locus_tag="IVO87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7448588.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42831..43484
                     /locus_tag="IVO87_000360"
     CDS             42831..43484
                     /locus_tag="IVO87_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7448589.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43547..44074)
                     /locus_tag="IVO87_000361"
     CDS             complement(43547..44074)
                     /locus_tag="IVO87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7448590.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44094..45041)
                     /locus_tag="IVO87_000362"
     CDS             complement(44094..45041)
                     /locus_tag="IVO87_000362"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7448591.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45053..46939)
                     /locus_tag="IVO87_000363"
     CDS             complement(45053..46939)
                     /locus_tag="IVO87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7448592.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47099..49177)
                     /locus_tag="IVO87_000364"
     CDS             complement(47099..49177)
                     /locus_tag="IVO87_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7448593.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49190..49465)
                     /locus_tag="IVO87_000365"
     CDS             complement(49190..49465)
                     /locus_tag="IVO87_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448594.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49827..50282)
                     /locus_tag="IVO87_000366"
     CDS             complement(49827..50282)
                     /locus_tag="IVO87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448595.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50436..51644)
                     /locus_tag="IVO87_000367"
     CDS             complement(50436..51644)
                     /locus_tag="IVO87_000367"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7448596.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51756..52088)
                     /locus_tag="IVO87_000368"
     CDS             complement(51756..52088)
                     /locus_tag="IVO87_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7448597.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52236..53111
                     /locus_tag="IVO87_000369"
     CDS             52236..53111
                     /locus_tag="IVO87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7448598.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53193..53774)
                     /locus_tag="IVO87_000370"
     CDS             complement(53193..53774)
                     /locus_tag="IVO87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7448599.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53780..55039)
                     /locus_tag="IVO87_000371"
     CDS             complement(53780..55039)
                     /locus_tag="IVO87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7448600.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55041..56087)
                     /locus_tag="IVO87_000372"
     CDS             complement(55041..56087)
                     /locus_tag="IVO87_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448601.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56322..56597)
                     /locus_tag="IVO87_000373"
     CDS             complement(56322..56597)
                     /locus_tag="IVO87_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7448602.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56840..58150)
                     /locus_tag="IVO87_000374"
     CDS             complement(56840..58150)
                     /locus_tag="IVO87_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7448603.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58332..59561)
                     /gene="rpsA"
                     /locus_tag="IVO87_000375"
     CDS             complement(58332..59561)
                     /gene="rpsA"
                     /locus_tag="IVO87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7448604.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59696..60376)
                     /locus_tag="IVO87_000376"
     CDS             complement(59696..60376)
                     /locus_tag="IVO87_000376"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7448605.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60425..61060)
                     /locus_tag="IVO87_000377"
     CDS             complement(60425..61060)
                     /locus_tag="IVO87_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7448606.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61126..62529)
                     /locus_tag="IVO87_000378"
     CDS             complement(61126..62529)
                     /locus_tag="IVO87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7448607.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62516..63547)
                     /locus_tag="IVO87_000379"
     CDS             complement(62516..63547)
                     /locus_tag="IVO87_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448608.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63578..63799
                     /locus_tag="IVO87_000380"
     CDS             63578..63799
                     /locus_tag="IVO87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7448609.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63964..64701)
                     /locus_tag="IVO87_000381"
     CDS             complement(63964..64701)
                     /locus_tag="IVO87_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7448610.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64854..66194)
                     /locus_tag="IVO87_000382"
     CDS             complement(64854..66194)
                     /locus_tag="IVO87_000382"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7448611.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66431..67954)
                     /locus_tag="IVO87_000383"
     CDS             complement(66431..67954)
                     /locus_tag="IVO87_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7448612.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68332..68943)
                     /locus_tag="IVO87_000384"
     CDS             complement(68332..68943)
                     /locus_tag="IVO87_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7448613.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69066..69191)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69273..69989)
                     /locus_tag="IVO87_000385"
     CDS             complement(69273..69989)
                     /locus_tag="IVO87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7448614.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69995..70564)
                     /gene="scpB"
                     /locus_tag="IVO87_000386"
     CDS             complement(69995..70564)
                     /gene="scpB"
                     /locus_tag="IVO87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7448615.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70548..71345)
                     /locus_tag="IVO87_000387"
     CDS             complement(70548..71345)
                     /locus_tag="IVO87_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7448616.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71315..71686)
                     /locus_tag="IVO87_000388"
     CDS             complement(71315..71686)
                     /locus_tag="IVO87_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7448617.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71705..72592)
                     /gene="xerD"
                     /locus_tag="IVO87_000389"
     CDS             complement(71705..72592)
                     /gene="xerD"
                     /locus_tag="IVO87_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7448618.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72688..73161)
                     /locus_tag="IVO87_000390"
     CDS             complement(72688..73161)
                     /locus_tag="IVO87_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7448619.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73266..74129)
                     /locus_tag="IVO87_000391"
     CDS             complement(73266..74129)
                     /locus_tag="IVO87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7448620.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74325..76424)
                     /locus_tag="IVO87_000392"
     CDS             complement(74325..76424)
                     /locus_tag="IVO87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7448621.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76644..77753)
                     /gene="rpoD"
                     /locus_tag="IVO87_000393"
     CDS             complement(76644..77753)
                     /gene="rpoD"
                     /locus_tag="IVO87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7448622.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77777..79618)
                     /locus_tag="IVO87_000394"
     CDS             complement(77777..79618)
                     /locus_tag="IVO87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7448623.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79800..82193)
                     /locus_tag="IVO87_000395"
     CDS             complement(79800..82193)
                     /locus_tag="IVO87_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7448624.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82300..83160)
                     /locus_tag="IVO87_000396"
     CDS             complement(82300..83160)
                     /locus_tag="IVO87_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7448625.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83219..>83467)
                     /locus_tag="IVO87_000397"
     CDS             complement(83219..>83467)
                     /locus_tag="IVO87_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HAP7448626.1"
                     /translation="KDYIAYVKNHGGENTVELAEAYSKDVLSPLLGNEEGKQYRYWRI
                     QSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
BASE COUNT        22944 a        17672 c        14213 g        28638 t
ORIGIN      
        1 attgcagaat caaattcaat ttctgatatt aacctgattt actcaggaca acaattaaca
       61 atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca
      121 ccagtccaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaaa acaagcagcg
      181 cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac tccagcaagc
      241 acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta cacagcaacg
      301 aacggacgtt acatcggacg ttaccaatta gattcttctt acttaaatgg tgactactct
      361 gctgcaaacc aagaaagagt agcagaacaa tatgttactt cacgttatgg ttcttgggaa
      421 gcggcgaaag cattctggga agcaaacggt tggtactaag attcatttat taatcaaaca
      481 gtttactttt atacaaagaa agccaaaaag aggtgcgaca ctagttgtgt cacatctctt
      541 ttttatttac ctaatctaat gaaacgttcc aagttttgat aagtattttt tttaacttct
      601 tcaactgata gctctttgat ctcagcgatt gtctttaact gttcttcaag cagtgagaca
      661 taatcttttt ctgaaaaaat cccccctaaa gcccactctg ctgcatgtag tccatccgtt
      721 tcaataagta agtggtcgat tgggactgct ttagctagtt gccagactgc tggattcttt
      781 ttcaaatcaa gaccaatgct gaaataacag cctaaatcaa tatagtcttg ttgatagtcc
      841 aaagaagaat accaatggat caaatagtga ttagggtaag cttggatata atttaagatc
      901 tctttttcgc atccttttgt gtgcagtaca acaggtttct catttatggc tgcaaaagca
      961 agctgtcgct caaatacttt tttttgtgta gtcatcggta cattcgtcca aatattatct
     1021 aaccctattt caccgatgat ccttgctttt ttcaaaaaag gctcaacttg ttcaaacgta
     1081 tagctatctg ctttccaagg atggataccg taacttaagg cttgagtctt acttgctgca
     1141 agctgtctgt tttccttcca ttcttctggc gaatcacaat tgatgatcgt caaaatcgtg
     1201 tgttcctgct gaaggagtgc taaactctga ctcccatcta aatgacaatg agcatcactt
     1261 aacatctaat tcacctgcca ataactgata aatcgtttgc ttcaactctg attgctgcag
     1321 tagccattca cgtgaccgtc gtttttcagg cacttgaatc tcagcaatca catgtgccgg
     1381 acgggcggaa agcacaagaa tcctgtctga taaataaatt gcttcttcga tatcatgcgt
     1441 cacaagtaaa gtagtccgct ttgctttttt aggcagcttc aacatccagt cttgcatctg
     1501 ccctctggtc atagcatcta acgctccaaa tggttcatca agaagcaagt aagaagaagg
     1561 attcatgatt gttcgtaaca aggccgttcg ctgcttcatc ccgccagata attgttccgg
     1621 caaaaaatgt tcatagccgt ttaacccaaa gatatccaga taatccctta cttgttcttc
     1681 attgattgtt agatgattga ttttttgata taacgtaacg ttttgataga cgctcagcca
     1741 aggaagtaac atatcttctt gcggcatata actaaatgct gtatggagac cagttacttt
     1801 ttgatcgtct actttgatcg ttccagaagt cataggctgg actcctgtta aagttttcag
     1861 caatgttgac ttaccggctc cgcttggcgc gacaatcgat gtaaaggtat ctttagggat
     1921 agtgaaatgg atgtcttcta atattttcag gttttcaatg actacattca agtggctgat
     1981 ttggattgac atatctatca ctcctatttt ataaactgat tagtgaatag ttcttgcgaa
     2041 ggtacatctt gcttgatcaa tccgttttcc tgcataaaag ctgtataatt gcgccaaact
     2101 ttttcttcca tcaatcccca attctcagga tcatccgtat aattttttga caagaatgct
     2161 tgtgattctt ctaaaaacgt ccgatcattt tcttttgcat atttttgcaa cagtttagca
     2221 ctgtcatttg gatccttaat agcttcctga tagccttttt tcgtggcatc cataaaagac
     2281 tgtaccaatt ctggatcact tttgatcagt tgatcattcg taataattac cggagtatag
     2341 taatctaacc gttcatctaa ttctttcaat gggatatagt ttagttcaat acccgcttct
     2401 tttgctttga tcatatccca accttcaaag taccattgga tatctgagga ttttcctaag
     2461 ctttctggcc cttctccatt gcttcctacc attgtcagtt ttgaaaagtc cccgccgtct
     2521 ttttccatca cagcttttaa gacagcttct tcacttggac tttgccaacc tgcatatgtt
     2581 tttccttcaa aatcttttgg tgaatggatg ttactgtctg atagggtggc gaatccagaa
     2641 gtattatgtt tgatcactgt agcaattgct ttgactggta tattctgtcc atctgcgtga
     2701 gcataagtga catcttcttg ataacttaca ccaaattgtg ctttgtccgc acctactaga
     2761 ccaatacttg tcgaattatc tcctggttcg atgatttgga catttaaacc agcctcttta
     2821 taatagcctt tttcttttgc taggtagatg ccagtatgat tagtattcgg tacataatcc
     2881 aaaacaagtg tcactttttt caaatccttt atttcttgtt tgctacttgt ttcttttgca
     2941 gtgccacaag cggataaaag caacagtagc ggtaataaaa gccataattt tttcatttga
     3001 tccccttctc ttaaaaaata taaagcatac gataataccc ttttttcaat aatgaacaag
     3061 cgccattcag caataaactc aatagaatga cacaaatgat cgcagcaaaa actttatcga
     3121 tctggtagct gttttttacg cgtatcatgt aataccctaa gcctgcttga gcgcttagcc
     3181 attctccaac gactgctccg ctgacacaat aagtagcagc cacttttaaa ccggaaaaga
     3241 agccggataa acccgctggt attttatata ttcgataaat cttccctgta tctgccccca
     3301 tcgttttcaa aaagatgatt tgctcttttg atactttcct caatgtatcc gaaaaagaaa
     3361 ctacgatcgg aaaatagctt atcaagacaa caaggatcac ttttggaagc ataccgaaac
     3421 caaaccatag ggtaagtaat ggccctaaca ccatgattgg caacatttgt gaaatcacta
     3481 gtaacggata aaaagacatt tctaaaaact tccaataatc cattgccaag gcagtaataa
     3541 aagaaagaac gctagctatt accaaaccaa gcactgcttc atttaatgtc gtgacagaat
     3601 gggaaagtaa tacttgtgca tctgccgcta atgcagaaaa tacagcagca ggtccgggta
     3661 aaataaaggc aggtattttc caaactatga caacaatctg ccaaataatt aaaatgaatg
     3721 ccatactgac tgctggataa agatttttct tcattttgtt acctcctttc ttctaatatg
     3781 aataacaaaa aacgcttgaa atgggcacga catagtaacc cgcttcaagc gtcggctatg
     3841 tttctccctt caccagtatt aactgacagg ttcaatgggt ctaagttcac aacttactct
     3901 catgtcgtct acgacatccc cagaaacagg tattcatttt ttaaagtgta gcacaagaag
     3961 gaaaaagcaa caagcgcttt agttatcatg ctgattattt caataaatag gagagatcac
     4021 ttgccaatgt cggatacccc atgatatagc tatccaactc ttttttagtc aatttttgat
     4081 tgatgacgag tgtcaaatca tcgatcaatt cgtctgcttg ctcgctaatc actgcagcac
     4141 caatcaaata attttgctgg tcaaaggtca attcagcctt agcaactggt tcattgacac
     4201 gatgataggt gaaccaactg gtaagatcca tctctaccac ttgatctgac gaagaggcgt
     4261 gactttttgt aactcctact tcagcaagtt taggactggc gtagacgatc gtaggtataa
     4321 tcggatattt gataggttcg cttgttgcat ccgttatccg cttagctaca taatttcctt
     4381 caaaagtggc gactggtgtc aattttgggg ttttccttga aacaatatct ccacacgcaa
     4441 aaatttttgg attgctcgtt tgcagataat cattgactgc aattccgtgt ttatcaaaga
     4501 cgatattcgc ttgctcaagt gctaaagatt ctgtattcgg ctgtctccca gtcgcacaaa
     4561 aaatcatatc tgctactaat tccgtctctt ttccgactaa acgatagcga gtaccttctg
     4621 aaatgatctt ttgggtttca acaccaaaag caaactgaat gccagatgct tccatttgat
     4681 ggacggcttc ctctaccaaa gacgcctcaa attcttttaa tggccgttga ttatgatgga
     4741 caatggttac tttgctccct gcagcattgg caatcgtagc tagctcaaaa gcaatataac
     4801 caccaccgat aaaaatgatt tctttaggca gtacaggcaa tgataaaaag tctgcactag
     4861 ttttcaagta ttcctttccc tccactggta agatcgtagg tctttgcccg gttgccaaaa
     4921 ctaagtaatc cgcatggaag acttcttcgt tgacttcaat agatgaatca tccaaaaaac
     4981 gagcggttcc tgaaagatga tcgatttcag cttctgctaa ttgttttttt cggctttctg
     5041 ggactggatc cgtaaatgtt cgtttgaatt tttgaagttc ttcccagttg gcagttggga
     5101 tctcgttaaa accttttcca ctcaattgtt ttacccgatt acgtgcctct actgcactta
     5161 acaagacttt tttaggatca catccacggt taggacaagt cccgccccat agatcttctt
     5221 cgaccactag tactgtttta cctgcttctt tcaatccata agcagcactt aagccgctca
     5281 cacctgaacc gatgatgatt gcatcatatg ttttcatttc cttcctcctc tcgacttaaa
     5341 aaagcaaaga tttctttact tgtcagcgac ccaatacttc tttgcttttc tactatcaca
     5401 atgcttggat acaccgaaag aagcgaatat acttctttca taggcgtatc aaagcaaact
     5461 tgttgacgag cattttctgg tatttcattt aagaaatatc cattgacgac taaatcttct
     5521 actcgccact ctttcgtctg tttcggttca tcaaagaaat gccgaacgta gtcgttttta
     5581 ggattcatca ataactgctc tggtgtgtca cattggataa tttctccatc cttcataaat
     5641 gcaatacgat cacctagctt gattgcttct tccatattgt gcgtaacgaa aacgatggtc
     5701 gtgcccaatt cttcgtgtaa ggacaaaacc aattcttgta atgagtttct tgaaatcgga
     5761 tctaatgcgc taaatggctc gtccatcaaa atcacatctg ggcttgcagc aatagctcgg
     5821 atgatcccga tccgttgctg ttctcctcca gacaactctc taggcatacg atctcgataa
     5881 atatcaggag ataagccaac tttttgaaga agttcatcta cacggtctgc tcttttctgc
     5941 ttttcccaac ctaacatttc agggatgact tcaatatttt gcttgactgt catggtcggg
     6001 aacaaagcga tttgctgcag cacatatccc atattccacc gcattttctg cagattatat
     6061 tcatttaacg attttccttt gaagtagatg tctcctgctg atggtttaga taatccattg
     6121 atcattttca aagtagttgt ttttccacta ccggaaggac caaccaaaac aaagatttct
     6181 ccttttttga ttgccagatt taggtcatcg atgacatact ttccatcata ctttttttgc
     6241 acatgtttga actcgattaa tttagtcact gatcttcctc ctttactaat ccttcatttt
     6301 tcaggaagat tcgtgctact tctgtcggct ttttcttttc gacattgact tggtaattca
     6361 tatcaatcat ttgatcctct gttatttttc cgcttaattt attcagtgct ttaacgattt
     6421 caggatgttc tttagcaaaa tcttttttca tcagcggtgc gccttgataa gctgggaata
     6481 accctcgatc atcttctaga gtcactagat cgtactcctt gagctggcta tctgtggaat
     6541 aggcatccac aatattgaca tctccattat tgattgcttg gtaacgcaaa cttggctcca
     6601 tgctttgtac acttgaaaaa ttcaagccat atgcttcttg gattccttta tatccatctg
     6661 aacgatcaat aaattccagt gtgaatcccg ctttgatctg atcagagata gctgccaaat
     6721 cggaaatctt tttaagttga tgttcgttcg caaaagatct cttgactgcc aaggcatacg
     6781 tattttggta agccattggt tttaataatg tcatatcgaa ttgcttattc aataattctt
     6841 ctgcttcgtc atatgttttt tcttttgtca aatcagcact tttcattgct tcaggtacct
     6901 tcaccaagct ttctaggaca gtacctgtga actcaggata gatatcgatt tgaccttgat
     6961 ccagtgcatt gaacaaaaaa ctcgtcttgc caaaattcgg ttttaattca acttctgttt
     7021 ttgtgttgtc ttcaatcaat tctttataca tttggatcag gatttcaggt tccgctccta
     7081 attttccagc aatcgtgatg gtttcatttt taaacaaggc agactgatat aaagaaacgc
     7141 cgccaaataa acacaccact gctgctaaag taatgatgga atatcgaggt ttcttatgct
     7201 gcaaccagcg aatcagtccg ctgaatataa ttgcaagcaa ggcagaactg atcgcaccaa
     7261 tcaatgtcaa aacaggtgta tttcggtcaa tacctaacaa aataaatgtc cctaatccac
     7321 ctgctccgat caatgctgcc aaagtagccg taccgatgat taaaactaag gcagttcgga
     7381 tacctgagat aatgacaggt aatgcgatgg gaagctctac tttcagcaag cgtctcattc
     7441 tggacatacc aaatgctacc gctgcttctt caatagacgg gtcaatttca gctagaccaa
     7501 tatacgtgtt ttggaaaatc ggaagcaaag cgtaaataac aagtgcaatc aatgctggaa
     7561 cagttccgat ccctacaaat gggataagta aacctaataa ggcaagtgaa ggaatcgttt
     7621 ggagcacacc cgtgatctgc aataagactt ctgccatttt tttatgattg gctgcccaaa
     7681 tcgctaaagg aatagcaatc agtgctgcaa tcagcaaaga aatgagagaa atggacagat
     7741 gttgaaatgc agcttccagc aactcgcctt ttctttcact taatgtttgt ataaaactat
     7801 tcatgcgacc ttcctttctg ttattcttta agtataaaca tttctttcct ttttcccaac
     7861 taacttgtct tgccttaacc ttacagcacg aacaaaaaaa agaaaaaaat acctctaaat
     7921 atgctgacaa aaaaagaatc caagcaacaa agtcggatca actttttggt tctcgttttt
     7981 tcactacgaa taccataaaa atctatacga aattgtcact tggaatcagc tatatctttt
     8041 ttacacacac aacttttatt ttaataaaaa gaaacagcca ataaagtaga aatctcgtgg
     8101 gggagaacac ctttattggc ctgttcaacg aatattatac tgtatcatga cacttttttg
     8161 ccaaaaatgt aaaaaatggt tgttttgaca tcataaacgt cttgttttct tatttttact
     8221 tatatcacca ctttattttc tatctctttc actccataaa gaggaatgag ttcttctttt
     8281 gactgataat aaacagcttt ctcacggttc ttcatcaaaa aaagaaagat gtctttcata
     8341 gcaagtggtg ctttgccgtg ttcaaataaa aagataaatt gatttgcttt atttatctgg
     8401 accattgtca caggcaccag tttttgttct tgagccaaaa aaactccttg atactctgtt
     8461 tttttgggtg attttgtaag aattgtcaat aagtctgctg ctcgcatttt tccactcctt
     8521 atttgataaa taaaaagaaa cagccaaaaa taattttttt tcattttcct cttgaagtaa
     8581 gagtataagt cagtataata aagaagttgc tgaaaatttt caaaagaaaa gaggctagaa
     8641 atgaaaaaat taaatttatt tacagaattg tgcggaatca ttttaggcgc aggaatctac
     8701 ggattagctg tgacaggaat caatattcca tctaagctgg cagatggtgg ggttactggt
     8761 attgctttgc ttttgcataa tctttttgga ttcgctcctt ctgtcacttc actgcttttt
     8821 aatttacctt tattgcttat ctcattgttt ctttttggaa aacacgcttt tctccggacg
     8881 atcgtcggaa catttgcgct agttttcttt ttacatttat gggagagcct tcatatacat
     8941 tttgctgtag atagtctact gatcaatagt ttgatgacag gtattctttc tggcattggt
     9001 tgcggtctcg tctttcgatt cggcggaagc acaggcggaa cagatatcat ttatcaagcc
     9061 attgaaaaat attttcatat taagattggc aaaagtcttt tcttgattac ttttggtatt
     9121 ttagttgtct ccttgcttta tttggatctc acacattttc tgtatacatt acttagctgt
     9181 tctattctat cttatacatt gaataaagtg aagtatctgg aaattggatc tgttttgccc
     9241 caattggaag caaaacagac tacagaaaca tttgaaccac ttgatcctct tgaacagtta
     9301 gagcactgat tcttattttt cactacttta tttaatttac agaaatatca ttatgtaaac
     9361 taaaatgata ttattcagta aaatcttctt tataaaaaaa taaactattt ttgcaaacga
     9421 tctgcgaaaa tagtttgttt ttttattttg ccattatttt cttacttatt gggagaaaaa
     9481 attcgcttaa actcaaaaaa atttgaggat acgatataaa ctttcggata gagtataaaa
     9541 taaggaaacg ccgcagcgtt tcctttccaa ctgtagccga atataaattt gtatatcact
     9601 tatttaaaat agctattttc tcccaaagag tttccttgat catcgatcaa tccttgtaat
     9661 gagcgttcta agataccatg gctttttttc gatttttttt cttctgactg ctttgtttcc
     9721 tctaacgttt ttgttcgatt gaatgttcgt acagacggct caccatttct tttttcttga
     9781 tacggcgtcg gttctgtatc gaacaacaga taagagtcat ccgttttttt catcgattcg
     9841 attaaatgat cctcagaaat tttgctttct ctagggtctg ggataatcga tgcgggtaca
     9901 tatttaggaa caaaataaga acgctcccgc accttcgtgt caacagatgc gtttcgtgta
     9961 gtatgcttgg actctgtcct tgtttcactt tgtctgacag aacgagagga atcaccaaaa
    10021 agtttttttc gtcctgtcga tgtgatttct gattggtaat ggccggtata atccggtaat
    10081 tttcctttat gcttttccaa attttcctta cgcggagctg acttcaatcc atttgttgca
    10141 cgacgcttgc gacggatttt ctcggtgttc gtgttcgtga aatgaaattc tttcatctgt
    10201 tcaaacgggg actgattctg ttcagtcaaa agatcttctt gtccgtcaaa taatactcgc
    10261 ttcttgtcgt tggttaattt tacgcctaac tcatcatcgt acgcaggaaa acggaaatgt
    10321 tttcgtctga tttctttcat tcaaaccatc cttctcaaaa atggaacttt tctcaataat
    10381 agcacagatt cattgttttc agtagcaatg cgctgattat tttaagaaaa tagcgatgaa
    10441 tttcataaat atatatatag taataagact taattcgttg tctctctgat aactagttca
    10501 ggaaaaaaac gacgataacc ttgactcgcc cctttttctt ctattttttt taacaacatt
    10561 tctccacata cttcacccat acgtataata tcttgcttga tcgttgtcaa tttaggtgaa
    10621 ctgatttggt ctaaaaatac cccatcaaat ccaatcacac caaattcttc gggcaagtgt
    10681 cctccagcat ttataatccc tcttactgct cctagtgcta gccgatctga actacataca
    10741 aaacaagttt tatctgtaaa atctgcccag ttttctgtca tatatctttc tacttctgtc
    10801 gaatgatttg gaaaccgcaa gattttacta ggaagatcaa cttccttcat cacagataaa
    10861 taaccagttt cccttgaaat ttcaaaaggc tcgtccactt cgataccgat ataaataatc
    10921 gtttgataat ctctttctaa ggcatattgc gttgcttttg ctgtaccata ttcattatcg
    10981 ctgtctacat aatcatatcc ttcagaattt tccccaaaca agacaactgg tttttccaaa
    11041 cggtcgatcc aatcgaagtc tttttttctg acacctgtga tgatgtatcc atcacaagaa
    11101 ccgacgtcga aggcatcatt tgtaactaat tggagagaat aatgcgcttt accgatccgt
    11161 ttagcgattc ccatcagcaa attcatgtaa tagggctcag ttgtgtcgat ttcttccagt
    11221 ataaataatt tgatgatctg cgaccgattc gtgacaagtg cttttgcagc aatatttgga
    11281 cggtaatcca gttcagccat tgctgagaaa actaactctt tcaattcgtc agtcacctgt
    11341 tccggatgat tgatgactcg agagaccgtc atttttgaaa cattggcacg ttttgcaaca
    11401 tctgctaaag tgggcatgct ctcttccttt ctaacatgtt gtcattctta ctatcaatta
    11461 taccaaattt tcctaacaga gtttattata gcccaaaata aaaaacggaa aatcctctat
    11521 cgtcatgagt gattttccgt ttcaattatt tatacttttt tatcaaattt tttgctaaaa
    11581 aagtggaagc ttcttccatt agtggtattt gtaccatatg tcgctgtgtt tttgaggaac
    11641 gaaaaacaat ttcttttccg taagacttat gccgattttt ttctacaaat cgttccgttt
    11701 gtatataagg gattttttcg tcagcttgtc catgccaaaa gaacagagga cgtccagcta
    11761 acttttccgg ctgcaatgac aaatcatagc tcttaaccca tgaaatcaaa tcaaaatagt
    11821 cttttggtac gacataatta tattccgatg cgcgccttac gatttctttt ccatattcca
    11881 aaggtgcagg ggaacccatc aaacacacgc ccgctactat atgaggatgt ttagccaaca
    11941 aggcgcatgt tgtcattccc cccatcgaaa ccccgccaac tgcaagctga tcatgtagaa
    12001 gacctctttt ttctaaatgc ttgataagtg tgtcaaattc aaacagattt ccgtagatac
    12061 tatcccaaaa tgtataggat ggaatagaag aaaccggctg tttgcgctcc ccatgattca
    12121 tcgcatctgg caggatcaca cgaatgccct gctgcgcaat ttttcttcct tgcgtcaaaa
    12181 ctaactcctt acttgtctgc catccgtgat aatagatgac aacgggaata cgttcctctc
    12241 ttaattcttt cagaacaact tctaacactg gaatatgatg gacagaacgt ttcaatataa
    12301 ttggattcaa ttgttactca cctattttca ttaaatttcc tgatcgccat agcaaccatg
    12361 ctccaacagt tgtctggaac aggttcagtg taccaaataa tataaatgca ggactgaatg
    12421 gctcgggaaa taagcacaga gaacaaagtc ccaccactcc taatgctagc gaaaggagtg
    12481 gataaataga aaaattcaaa aaactggcaa cgctaccgaa aatattacca ataaacagta
    12541 tcaaaaagaa aaaaacgctt aaaattaaac catctgtcag cgacatctga aaaacaatgt
    12601 tctctacctc aattggctgt tgaatcgaat agacacctaa gtatatactg agaaggagac
    12661 ttagcacgtg gctggcacaa aacacacgat tgaccgtttt cattttaatc acctaaccta
    12721 tctttataat tctattataa tcattttatc atttttttgt atgtataaag tttcaatttt
    12781 tcttcactta cgattttttt tgatattgtt gttatataaa tgaaaggagt tttacaaatg
    12841 attgatccaa aaatgttggg gatgatcttc ctcattaatt ttgcttatat cactctgaac
    12901 acgttacgtt tcatgttgac aatgaaaggc tatcgactga ttgctccact tgtcagtatg
    12961 gtcgagatca cgatttatat cgtcggctta tcccttgttt tagatcgttt agataatcca
    13021 ctgaatttat ttgtctatgc gttaggttac gcagtaggta tcagtgtcgg aattttgatt
    13081 gaagacaaat tggcattagg atacatcatg gtcacaacga ttttgccagc aaatagcgca
    13141 gaagataaaa gtttgccggg tatactgaga gaacacggtt atggtgtgac acagacttct
    13201 gcaatggggc tggaaggtga gcggctagtt ctcgaaatac tttctcctag aaaaagcgaa
    13261 cgtgaactgt atcgcctgat caaagaaatc gaagagcgtg cttttatcat ttcttatgag
    13321 ccaaaatata tttcaggtgg tttctggact aaaaaagtga gaaaacgaca aggaaaataa
    13381 ctcacaaact taaaaactgt ctaatatcta aattaaattt taaatgttag acagttttta
    13441 tttattattt tgattaaaaa acatcgacat atactcatca atttgttatt atactttata
    13501 tacttatcaa tttaatttgg agatattatg gaaattcaac gtagaaccac taatttttta
    13561 catttaggct ctttttttgc aactttgacg atcttatttt ttctatgtta ttcatacctt
    13621 aagacctcac atgcgccttc tagtgcatta ggctggttgc ttttggatca ttctgatatc
    13681 acagaagccg aagaaaatcc gttttttttg attttgtctt ctctttgcgg cttactgttt
    13741 tctgtttatt tcggtgccta tttttttcat cgacatcctg gtacgttaaa cgaagcttca
    13801 agcgtattga ttgcatttaa aacgttttct tttttagcaa ttctttttta ttcgtttaat
    13861 cacaatacgc ttttcttctt tatcatgaat ggtttagtcg tgattttatc catttttact
    13921 tattttattt tttcactcat ttttagccaa ttgaaatgtc cgctcttctt tcgattagtt
    13981 cctgtcacct gttttattta cagcttgttt tccttttttg tccttgcttt aatgccactg
    14041 accaaccctt cgacgatcca agtcgcagga aatctgttgg atatgctctt catcgtcagt
    14101 ctaggtgtat ttatgtggca aagaaaaaac aaacaaaata aaaagcattt tgaagcctga
    14161 ctattacgac ttcataaatg cttttttagt tttaaacact tcaatctaaa agaataaccc
    14221 aagattggat aaattattaa tgactcgcca ttcgtttctt agtgattctg gattatcaaa
    14281 tggacttcct aatctaatca gctagttctt cccataaaat gccatacttg actgaatcat
    14341 aatattgatt atttaaaaat ctaacttttc ttatcactac ttcctttgtc attccacatt
    14401 tctcaccatt tttttgcatt cctttgtttc cagaccatgt agtgaatcta ataggtggta
    14461 gataggcaaa atactcaaat aattctttta accataaagg aagagaaagg ctcctcctgc
    14521 aagagtcagt ataatttttg cttactgctc tcttaggttt tactcactaa aactgttcca
    14581 tattctttga tataagaaaa tgcatgcggt ataaatttct tgctcagctc agaaaagatt
    14641 tgtcgtttaa gaaaggccat ttcctccatt gtcaatgaag gatccaatgt tggtaattca
    14701 atctcaaaat tgtttttttc gacaataggt aattctaatg tgaatgattc ttcttccttt
    14761 atagtattcg tcacaagtag agactgatgg aaaatactag gccggcgatt tttatattta
    14821 ttcttacgta tcctgtcaac gataacttct atttgatcaa aaaagtacga ttcatttacg
    14881 tctccaataa gttcggctaa ttctttgaat gccttcgtta caggaagctg attcttctgt
    14941 acaaaaaagt aattagccaa agtaagcggg aagtgttcta tagaggaaag tgtgataaaa
    15001 cggtacttct catttcctgc agttgtcctc agcaattctg tctctcggct gtttcttact
    15061 ccataaaaat acgcttcttt ccgttcatct tcgaaacaat acgcccaaat aacttctccc
    15121 atcgctaatt tttgttcatc aacagagagt ctcttcaatt gttcagctat ggtttcagca
    15181 gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt caaatgatat
    15241 cttcgttctt ccctcttgat taatcctgca tcgatcaata aatcaagaaa atgggaaaat
    15301 ttattttgcg aaaattcttt tttcaattct cttagaatcg tttcttgttc tcgatgttca
    15361 tatagataat cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt
    15421 tccttatttt ttgaataata gttcatatat gttctctttt ctatccgttt tattttactt
    15481 aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat aattgactgt tattctcctc
    15541 catttcttcg gcctttagta tgttctatgt ttctttttat atcaatcgct tatccattca
    15601 ttttgaatgc ttcatactac catttgatta gctaaataca gccgtcaatt gaggtacgac
    15661 ttgtttttta cgtgaaacga cacctttaag caaagcacgg ttgttggtca aagttgtttg
    15721 gaaagcttct tctacttttt cttttccttt accagcaaca agtaattctg aatcactgtc
    15781 taagatgttt gtgacaacta atacgaacag atcgtagcca tttgctttca tttcatcttc
    15841 cattgctgct tctagctctg cttgtcgatc catcacttca ttaaggtcca ctgtattgac
    15901 ttgagcgata cgaacgttgg aactgcccat tgggaaactt tttgcatcca aatcaagaag
    15961 aaccgcagct gatttatcac ttagatttgt tcctgctttc aacatttcaa gtccataagt
    16021 gtttagatcg acttcagcga ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt
    16081 acatgttggt gatttgaaca acaatgtatc tgaaatgata gctgagagca tgattcctgc
    16141 gatttcttta ggaattgtta cttggttttc tttatatagt tttaaaataa tcgtacttgt
    16201 acatccaact ggttctgcac gatagtacaa tggattagct gtttcaaagt ttgcaatacg
    16261 atggtggtct actacagcta aaatatttac ttcgctgata tctgaaatac tttgttggaa
    16321 ttcattatga tccacaagca tgacatctgc attttctgct ttagctgact ctactactcg
    16381 tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt tcttcattag gtgtacctag
    16441 agcaactgct tctgtttctt tacctaattc attttgcaag tgtgaaaacg caatggctgc
    16501 cccgattgca tctgtatctg gattttggtg accaaaaact aaaatttttg acatgtcatt
    16561 cgctcctttg tgatttctcc tatttaataa tagcaaaaaa acggaaagaa gcaatactta
    16621 tcttctttcc gatgcgacct atcgcgttaa atattttgta tactcttctg tatgaagcaa
    16681 tcgtttagca ttttcgacac gttcttttgt aggtggttcg atcccttcta atggatattt
    16741 caaaccaagt gtttcccact tgtattttcc catcgtatga taaggaagaa tctctactct
    16801 gtctacattt gacaaggttt gaataaagtt gtctagtcgg atcaaatatt catcataatc
    16861 acttcgctca ggaactagta catggcgaat ccaaaccggt ttgttgattt ttgacaaata
    16921 ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt agttttttat gctcttcatt
    16981 atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt cttgaaaacg
    17041 tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat gtatccaatg tagtatgcat
    17101 cttccgctct tttgcttttt taaatagttc gatcaaaaaa tcgatctgca atagaggttc
    17161 tccaccacta acagtaatac cacctttatc tccccagtaa ggtctgaagc gttctgcttt
    17221 atctaaaagt tcatctgctg tgtattcttt ccctccgcca atattccaag tatctggatt
    17281 gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac acgacaaagc gcagtccagg
    17341 tccgtctact gaaccgaaag tttcgataga atgaacatag ccgattgttt tttcttccat
    17401 ttaagttcac atcttttctt tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc
    17461 tttctgaaca aaatttttcg caattgccgc ttcgttatgt gtaaaattta cagggacgga
    17521 acatacaagt cctgcccctg taaatcgttt ctttcaaata agcatactga ttattcgatt
    17581 ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac
    17641 tttttacatt ctgtcgtgtg aagttctcat caagacatct aattgttgtt ctcttgtcaa
    17701 gtcacggaat ttaactgcat aaccagatac ccggatcgtc aagttcggat atttcattgg
    17761 attttccatt gcatcacgca ataaatcatt actgaataca ttgacattca aatgataagc
    17821 tccttttgag aagtatccat ctaagacatt acgcaagttt tcgattcttg tatcttgttc
    17881 ttttccaaga cctgaaggat tgattgtttg ggtatttgaa attccatcca atgcacagcc
    17941 gtaattcatt ttagctgttg agttcaggct agctaataac ccactttgtt ccgcattgta
    18001 acttggattt gctcctggcg caagtggttt tccagcttca cggccatcag gagtatttcc
    18061 tgtattttta ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg ttggttttga
    18121 gttgcggtaa gttttatgac gtttgatttg tcccatgaag tattccaaga tccatgaaac
    18181 taattcatct gcacggtcat catcatttcc gtatttaggg aagtcgccat ccacttcaaa
    18241 atcaactgct attccttttt cattacggat tgcttttact tttgcatatt tgatcgctga
    18301 caagctgtct gccgcatgag agatcccagc aatacctgta gcaaatgtgc gttgcaagtc
    18361 agaatccatc aaagccagct gagctgcttc ataagaatat ttatcatgca tataatgaat
    18421 gatgttcaac gtattgacat atagttctgc taaccaatcc atcatattca tgaagttatg
    18481 tttcacttct tcaaagtcta gtatttctga tgtgattgga cgatattctg gtccaacttg
    18541 ggcgccagtc acttcatcga ctccgccatt gatggcatat aatagtgttt tagccaagtt
    18601 agcacgagcg ccaaagaatt gcatgtcttt tccgacaact gttcctgata cacagcaagc
    18661 aatcgcacaa tcatcagaac cccattgttc acgaagctga ttatcatttt cgaattgaat
    18721 cgaagaactt tcgatagcga ttctgctagc atatgttctg aatccttctg gaaggtgttc
    18781 agaatacaaa acagtcaagt ttggttctgg tgaaggtccc atatttgtta atgtatgaag
    18841 cagacggaaa tcattctttg taactaatga acgaccatct aatcccatac ctgcaatgga
    18901 taaagtagcc cagataggga atcctgagaa taattggtta tattcaggtg tacgcgcgaa
    18961 tttgaccata cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt
    19021 gattactcca ttatccaaat cacgttggat atagatatca aggaatgctg agatacgtcc
    19081 gattgacatc gcagcaccat tttgtgattt gattgctgct aaatatccga agtatagcca
    19141 ttggacagct tcttttgcat ttgcagctgg tttagaaata tcatagccat atttcgcagc
    19201 catttctttc atttgcgcta atgcacgata ttgttcagtg atttcttcac gtaaacgaat
    19261 cacttcttca ctcattgtat gataaccagt catatcatga tcttttttct tttgttccat
    19321 caaatagtcg attccataca atgccacacg acgatagtca ccgatgatac gtccgcgacc
    19381 gtaagcgtct ggaagccctg taatgatttt gtttttacga gcagcacgca tttcagcagt
    19441 ataagcgtca aatacacctt gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa
    19501 ttcatcgttt ggtgtatagc cattgctttt tagcgcattg ttagccatat tgattccgcc
    19561 gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga ttttttccaa
    19621 atctttatcc aaatagcctg catcgtgtga agtaatagtt gatacgattt ctgtgtccat
    19681 atcatagaca ccgttacgct cgaattggat atcatttaac ttttgcaatt catcccataa
    19741 tttttctgtt gcttctgttg ggccttccaa aaagctgtca tcaccgcgat attcagtgta
    19801 gttattttga ataaagtctc tagtgtctac gccttctttc catttggtac ctttaaatcc
    19861 attccattgc tccattgtat ggacctccta aatttctgtt ttatgtaaca gtttttacgt
    19921 gattagtata acacagcacc ctatctttgc aagggttttc ttgtttttta tttaacaatt
    19981 tttatgtttt atttacatat atttaaatca acgttgacat accaacattt aagcattgtg
    20041 aacactttca tctttttttg caaacgtata aggatttatt aaaaatgaat gaaaaagaga
    20101 cgccacctgt attaatacag atggcgtctc ttcattaaaa tactacaaca gttacgactc
    20161 gatgaaaatc tcaggagcat cgataacttc gatgacttct ccaccttttt tctcatccat
    20221 cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa tcagttgggt caatcgttaa
    20281 ctgctgccct ttttgattca tcaaggttat ttcttttgat cttccttccg acatatagat
    20341 aatacggtgc ggattttttt tcagctcacg catgaccgtc actccccgct tcgcacgtga
    20401 agtttggctg atttcttgag caagcatgcg tttaacagct cctcgctgag taacgattac
    20461 gatcggcgta tcaccttctg ctaacacaag cagcccgttg ataatcctat ctccatcttt
    20521 caaattcata gacttcactc cagctgcctt tgctccaaca acgggaacat ctgttaaagg
    20581 atagcgcaaa cccattcctt gatgcgttac taagaaaaca tctaagcctg gctgatcttc
    20641 tttcagatac acagcaatca gttcatcttc ttccgccttt agcttcatac agctgagcgg
    20701 acggctctta taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc
    20761 attcttgctg atgaatacaa atgttttctc tgcttgaatt ttttgatatg ggaaaacagc
    20821 taaaatcgat tcgtctacag acagattgga gattgtttga gaaatgtgct caccagcatc
    20881 tttccatttc aaatcgggta gttcatggac agggcggtag atcacatttg ctttatttgt
    20941 gatcagaaga acatggtcca atgtactcaa ttcacctgag tatagcaagt agtcgccttc
    21001 tcgcatcccg atttcttctg gtttggaagc actataagaa cgaaggcttg ttcgcttcac
    21061 atagccttct cgcgtcacac tgacaattac gtcttcttgt gccactaaga cttgcgtatc
    21121 aattttgatt tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag aataattttt
    21181 tttcacttct cgcaattctt ttttcataac agagaaaagc tctttgtcat ttgaaaggat
    21241 tttattcaac tctgtgattt gcgcaatcaa gctttcggat tccttttgaa gctctgtgat
    21301 atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt gttcttctgt
    21361 aaattgaaag acgtcgacta aattcttttt ggcgtctttt ttgtctttac tttcacgaat
    21421 cgttgcgatt acttcatcca gaatcgataa agctttcatc aatccttcta cgatatgctg
    21481 tcttttttga gctttggcta aatcgaattg actacgtttt gttatgactt gctttcgatg
    21541 ctcgatataa cttcttaaaa tgtctttcag tccaacttga tgcggagtca tatggtcgat
    21601 agcgaccata ttgaaattgt aattgatctg tagctcagtg tttttgaaca aataattcaa
    21661 aattccttga gcattggcgt cttttttcaa ttcgacgaca atttgcagac ctgttctatc
    21721 actttcatct cgtacttcag ctattccatc gattttctta ttcaaacgga tctcatccat
    21781 ttttttcacc aaggtcgctt tatttacttc atatgggatt tctgtgatca cgatttgctg
    21841 tttattaccc ttcattgcct ctatctttgt tttcgaacga agaataactt ttcctcgtcc
    21901 tgtttcataa gcttttttga tttcatcttt tccttgcaag atccctcctg ttggaaagtc
    21961 aggtccagga atatattcca tcaatttttc aaggctcgct tgtggatgat cgatcatata
    22021 gattgtgcca tcgatcactt cagctaagtt atgagtcggg atttctgttg catatccagc
    22081 agatatacct gtcgatccat tgacgagaag gtttgggtat cgagctggaa ggactgttgg
    22141 ttctttttct gtatcatcaa aattccacac gagatcgact gtttctttct cgatatctgc
    22201 tagcatctct ccacttaact tggataatct ggcttcagta taacgcatcg cagcaggtgg
    22261 atcaccgtcc atactcccgt tattcccgtg catttcgatc agtacttccc gcaacttcca
    22321 gtcttggctc atgcggacca tcgcttcata aatactgatg tcaccatgag gatgataatt
    22381 ccccatgata tttccgacag attttgctga tttacggaat cctttgtcat acgtattgcc
    22441 atctttgttc atggcaaata atatccggcg ttgcactggc ttcaatccat cacggatatc
    22501 aggcagtgca cgttcttgga taatatattt ggaataacgt ccgaagcggt cacccatcac
    22561 ttcttctaaa gttaattctt gtacttcttg tcgattttcc atcaggtgac ctcctattct
    22621 acttcaaata actgattgtt ttcattttta tctgctcgtt cttcatctaa taaatcatta
    22681 gaaactgaag gagagatttc agtatcctct tttttatcta agatactgcc atcttcttcg
    22741 agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag gttctacttt gtcacccatc
    22801 agtgtggtta ctcgacgttc agcttgcgca gcatcatcga tacggacgcg gatcaacgta
    22861 cgggaagtcg gatccatcgt tgtttcccac aattgttccg cattcatctc tccaagtcct
    22921 ttatatcgct gcagcatata tcctttgccg acttttttaa tcattgctgc caattcgtca
    22981 tctgtccacg catattcaat gacttgcttc tttccttgtc ctttggatac tttatataaa
    23041 ggcggtaacg cgatatatac tttcccagct tcaatcagcg gtttcatata acgataaaag
    23101 aaagtaagca gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt catgatgatt
    23161 actttgtcgt aattacagtc ttcgatagaa aactcaggac cgactcctgc accaatcgta
    23221 tagatcatcg tattgatttc ttcatttttc aagatatctt gcattttagc tttttcggta
    23281 ttgatgactt ttcctcgtaa aggtagaatt gcttggaact ttctgtctct tccttgttta
    23341 gcagatccgc cagcagaatc cccttcgact aaatacaatt catttttctt agggtttcgg
    23401 gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc cttttttacg cttcttccca
    23461 tttcggcttt cttctctggc tttacgtgct gcttctctcg cttctctggc tttgattgct
    23521 ttacggacca acatctggct catttcgcta ttttcctgca aataaaagcc catttgttca
    23581 ctgatgacgt tatctactac ggtacgagca actggcgtac caagtttttc ttttgtttgc
    23641 ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa gtacagcagc taacccttct
    23701 ctgaaatcac taccttccag attcttgtcc cgttctttca gcaaaccaac ttttctcgca
    23761 tattcattat aagatttcgt catcgccgct ttcataccag cttcatgggt tcccccatct
    23821 tttgtccgaa cgttattgac aaaagataaa acattttctg aataaccgtc attgtattga
    23881 taagcgacct ctacttcgat accttctttt tctcctgaaa aataaacaac tggtgtcaat
    23941 gtatcttttt cttcattcag ataatcaaca aattctttga ttccttcttc gtaatgaaaa
    24001 atttccttga ctggttcttc tccacgaaga tcagaaagag agattttaac gccttttaga
    24061 agaaaggcag attctcttaa ccgttcggct aaaatttcat atgaaaattt cgttgtactg
    24121 aatattgtat catctggtaa aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct
    24181 atctttttca aagtaccgtc aggtttaccg ccgtttttaa atttctgctg atactctacg
    24241 ccatctctaa cgatcgttac aattagccat ttcgataagg catttaccac actggctcct
    24301 acaccgtgca gtccaccaga agttttgtac cctccttgac caaactttcc gccagcatgc
    24361 agcacagtaa aaatcacttc aactgttggt attccggaag catgcattcc tacaggcatc
    24421 ccacgtccgc tatctgcaac agttatacta ttgtcttcat ggatggtcac atcaatctca
    24481 ctgccataac ctgataatgc ttcgtcaact gcattatcga cgatttcata gactaaatga
    24541 tgcaagcctc ggctgtcagt ggatccaatg tacatccctg gtctttttct aactgcctct
    24601 aatccttcca atacttggat ggacgagtca ttgtattcgt tgtttacttt ttttgccaag
    24661 gcaaacgctc cttcaataat actttgcggc aaaaccgcac cacgattatc atactagaaa
    24721 aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt aattatctcc taaccgcttt
    24781 ttcgacattc ccctctatcg atcatctctt ctttgtggtc atttttattt ttggttttct
    24841 ttcaacttac caagttcaat cttaatacaa cgatccatta cgatatctgt acgtcctgct
    24901 tttcttagca tctcagctgc ttctttgttt tcaatcccta attgtgccca gaacacttta
    24961 gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa attcactccg tctaaataca
    25021 tctacgatat cgattggatc atcgatttct gtcaaattcg catagacttt ctcgcctaga
    25081 atttcttcac cagccaataa tggatttact ggtatgattt tatatccatt gtcttgcaat
    25141 acctgtgcaa ccatgtaact tgttcgttct ggtttattgc tcaagcctac gacggcaata
    25201 ttttttgctt cttccaaata gttgaaaatc gtttcttgtg ttggattttt tacagacatt
    25261 tctgccaccc cttgcttttc tttttattcc atctattata cttgtatctt tcattttttc
    25321 aattgttcct gttacctatt catgctaaaa taaaggatag ttttcaactt gaaagagggt
    25381 aaaaaatgaa attgatctta ttacttattt tagcatatct tttaggttct attccttccg
    25441 gtgtctggat tggcaaattg tttttcaaaa aagatatccg acaacacggg agcggtaata
    25501 ctggaacgac caatacattt cgtgtattag gcaaaacagc cgggattgtt gtcctgctta
    25561 tggatatttt aaaaggtaca ttggctactt gtttgccgat gatcttccac ctttctggga
    25621 ttaaccctct ttggtttggg gtatgcgcca ttcttggcca tacatttccg atttttgctg
    25681 ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat gcttttagga tttaatcctg
    25741 tattttttgt ttattcttgt atcatcttta tcgttagttt attttgtaca agtatggtga
    25801 gtttgagcag tatgatcagt gcagtattga tcactttatc tacgatcgtc cttccttatg
    25861 tagctcctgt gattttggct aagcccaatt ggttactgac gatcattgcc tttttagttt
    25921 cttcctttat cttttatcgg cataaagaca atatcaagcg tatcagaaac catactgaaa
    25981 gtcgaatccc gtttggtttg aatagcagca agaaaaaatc ctgatacttt ttcattccaa
    26041 agagataaaa cgggagtgtg acaaatcgat gtcacactcc cttattactt acaggatagc
    26101 ttttttacac accttggtaa acggtgttcc ctgctaactt ctcggtcaca acctcttact
    26161 tcactgtgat ggaatatttt gtcttgaaga tttcatgggc acctagttga ttgatcccta
    26221 ctttttccat caagtttcca ctactgttca ccgtgtcagc gattccgcac cacggttcga
    26281 tacaaacgaa aggcgcttct tttggataag gagaccagat ccctacataa ggcatatctt
    26341 tatagcttaa agttacgcta tgggaagatt tttcacttcg aatggcgtac gcatttagac
    26401 cccttgtttc aaaaaccaag gcatcttgct caaaaagttc acgattcaaa gccagattcg
    26461 tatttgtctg acctaacgtc ttttgatcca tatcgatgaa tgggcctttc aatggtaggc
    26521 gtaatcttga tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac aatccttctt
    26581 caagaggtac attgaaagca ggatgtccac cgatagaaaa gaacatttct ttttcaccgg
    26641 tatttgccac ttgataatga accgtgatgc cgtctcctcc taattcatag ctgatcacta
    26701 attcaaaatc aaatggatac ttttctaatg tctccggcgt actccttaag atgaaactag
    26761 ccatttcttg tccatgttca atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc
    26821 ccatttgata tgttttccct tgataaacat actgatcatc ttttaaacgt ccaacaaaag
    26881 ggaataaaac cggtgcatgt ctgccccaat acgctggatc accttgccag atatactcta
    26941 aaccatgttc ttttgatttc agactgatca attccgcacc gtgttcagcg atttcggcga
    27001 tcagctggtc attttgaata ataacagtca ttgccattcc tccaacttcg acttactttt
    27061 taaaatggga aggctggaac aaacatttct atctgcccca gtccccttac ttctaataac
    27121 tgtgttttgc taactcgtct ttaaaacggg aattcaatac ttttaaataa gttcccttca
    27181 tgcctaacga acgagattca atgattccag ctgattccaa ttttcgtaaa gcattgacga
    27241 tcacagaacg agtgatgccg atactatcgg caatattaga agcagtcaga cgaccttcat
    27301 ctccatccaa tgcttcaaag atagcttgta cagccttcaa ttcgctataa gataatgtgt
    27361 tgattgccat ttggacagcc gttgcactcc gtacatctgc ttcgatgctt cgagacttct
    27421 gataaaggat ctgcataccg acaaccgtag ctccatattc tgctaagact agatcgtcat
    27481 ctgcaaacga ttgttccata cgagataaga taatcgtacc caaacgttcg ccagctccaa
    27541 agatcggaac gacagtcgtc agaccaaatg gatatttctc tcttgactct accggaaatg
    27601 cagtaatatc actagtaatt gggatattag cttcagtttt catcaactgt gccactgtat
    27661 cagtataaga ttcaggaaat tgtttctcaa caaacattgt tttgattcgt tcattgttca
    27721 catcgtgacg ttcattataa cctagtaata ctccgtcact tcctatgata taggtgttgc
    27781 tgtcaagaat accacctagt gtgacagcca ttttatcgta aggcaaatct gcttgcatgt
    27841 caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg
    27901 tcattgtcgt cctccgattt tttataaaat atagcgactt aaatcttcat ttgccgcaat
    27961 tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt atttctccca tttgcatatc
    28021 tggtgcttca aataatagat cttctagtag tttttccaaa atggtatgca gtctgcgtgc
    28081 gccgatatta tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc
    28141 ttcttttgta aacgtaactg acacattttc tgtaccgatc aacgcgacgt attgttttac
    28201 taaagcatta tttggttcgg tcagaatctt cacaaaatct tcagctgtca aatcatctaa
    28261 ttctacccgt atcgggaagc gtccttgaag ttctggaatt agatcacttg gtttagacaa
    28321 atggaaagct cctgaagcaa tgaataggac gtgatctgtt tggatgatcc cgtattttgt
    28381 gtttacttgt gaaccttcga caattggcaa tatgtctctt tggacgcctt ctctggatac
    28441 ttctccagat tgctgtgatt tagaagtgat cttatcgatt tcatcgataa aaataattcc
    28501 actttcttcc gctaatttga ttgcctcact atggatatca gcatccttga ctaattttgc
    28561 tgattcttcc ttgatcaata actctaaagc ttctttgact gttaccgtcc gttccacttt
    28621 tttcttcggt gtcaaagcac ctagagtttc atttaggtcg atccccattt gctccaatcc
    28681 attgttcatc attggtgcag gtttcttttg gtcttcaact tcaatggtta cttcacgact
    28741 atccagtaaa cccttttgta gttgttccaa gactgtacgg cgattgattc gaatatcttc
    28801 tgtcacttct tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa gattcatcat
    28861 ttgttcaaat tgattagtgg attgtttctg ttcttttttg ataccaggta ccaagatttt
    28921 taccaaacgt ttttctgcat tttttttagc acttgtgtat acacggctgt actgctcttt
    28981 ttctacgatt tggatcgcat tttctactaa atcccgaacc attgattcta catctcttcc
    29041 gacataaccg acttcagtaa attttgttgc ttcgactttg acaaatggtg cattgacgat
    29101 ttttgccaaa cgtcgtgcga tttctgtttt accgacccct gtaggtccga tcatcagcat
    29161 attttttggt gtaatatctt gttgcatttt ttcatctaac tgcaaacggc gatagcggtt
    29221 acgcaaagca acggcaacag atttttttgc tgcgtgctgt cctacgatat attcatctaa
    29281 ttcacttacg atttgtcttg gtgtttgttt taattcatac atatctactc acctcttaca
    29341 gttcttctac gataatatta tgattagtaa agatacagat atctgcggca atatttaacg
    29401 cattttctgc aatttctttc gcactcatgt cgttttgagc atagtttttc attgctcggg
    29461 ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa gataccatca tctggtgtga
    29521 taacttctcc tgtacctgaa acaagaagca tttctttatc attcattacg atcagcatcg
    29581 cttcaagttt ttgcatagat tgctgtgtcc gccattcttg agctaattcg acagctgcgc
    29641 gttgtagatt cccgttatat tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg
    29701 catctgcaac acttccggcg aaacctacaa caacttctcc attgtagata cgtcgtactt
    29761 tctttgcagt gcctttcatg acaacagatt cacccattgt tacttggcca tctccagcca
    29821 tagcaaattt cccgtctttt tcgacggcac agatagtggt tgaatgaaat ggtgattcga
    29881 ccatgtttgt ttcctcctaa atttgttctt ttccttattt ttacgctctt ggatggaaag
    29941 aacgatagtt tttttgtaaa ctttcttttg ttacatgggc atagatctgg gtagtcgata
    30001 aattggcatg tcctagcaat tcttggactg tccgcaaatc tgccccatta tttaataaat
    30061 gagtagcaaa tgtatggcgc agcatgtgag gatggatatc actatccaaa ctgctttttt
    30121 tgatcatttg attcaagaca tattcaattc cagtaggcgt gattggtttt ccatgatgat
    30181 tcacaaaaac gatatcatgc tcttcgtgat attttgtcat caatacctgg cggccttccc
    30241 tgaaatattc ttgcagagca tcttgagcaa aagaacctag aggagcatat ctctctttgt
    30301 tccctttacc atggatcaac atgacctctg aatcaaaatc aatgtctgat accgacaaac
    30361 tgctacactc acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc
    30421 tcaaatccaa aggactatct cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat
    30481 aaaaaaaacg tggcagtctc aaatttttct ttttgagatg aacgtaagaa aatggatttt
    30541 cttttatcac ttcttctttg agaagatact gataaaacga acgtaggctg gctatcttgc
    30601 gactgataga atttctgcta tagctaggac gatgatcagc taatgcactc aaatagacac
    30661 gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt
    30721 tgaaatggag aatgtcttct tcatatgctt ctttcgtttt ttctgaatat cctctttcga
    30781 caatcaaata acgcaaaaat ttttgttccc acgtggcttc tgtcattttc tcaccttcct
    30841 tttgtcccag agaataattt agcacagcgt ttataaaaag acaaataaat tgtcagaatt
    30901 taaccaatat ttctaagttt gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa
    30961 ttcttaagaa aaacgcaaga aaaagagcga gactatccgt ctcaccctct tcatggagtt
    31021 tccttactca tttgggattg cagttttttc taattctgtt tttattgcct cattagcttt
    31081 taaagctcgt tgtgcaattg cttcatatct tgcttttttg tctcgaatgc gctctggcag
    31141 ttcaggaaac agaccatagt tggcattcat cggttggaaa tgttttcctt ccgcatgagt
    31201 aatgtagtaa gccatacttc caatagctgt ttcttgtgga aactcgactg gttccatttc
    31261 ttttgctaag cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac tttcaacata
    31321 gccttctaca ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg ttgattggta
    31381 agtcggtttc agcaattctg gcgaattcat aaagctgttt cggtgcatca caccatagcg
    31441 aacgaattca gcattttcta acccaggaat catccggaaa acacgttttt gctcgcccca
    31501 tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg
    31561 cagctggata acagcataag gacgtttgcc agttttagga tcttccaatc caactggttt
    31621 cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa ttggcataca
    31681 tccttcaaaa tatttttctt tctcaaaaga tttaagttct gccacttcgg cattgattag
    31741 tgcctcatgg aaagctttga attcctcttc tgtcatcgga cagttcaagt aagctgcttc
    31801 tcctttgtca tagcgggact tcaaatatac tttatccata tcgatcgtag ctttgtctac
    31861 gattggtgca gctgcatcat agaaataaaa accatctgaa ccattgaatg ctttgatgct
    31921 ttctgctaat ggatgagaag tcaaaggtcc tgttgctact acggtgattc cttccggtat
    31981 ttcagtaatt tcttcgttta tcactgttac taatggatgc tctttgactt ttcgggtaat
    32041 tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca ccagctggca ctgctgtttc
    32101 atctgccgaa gtgattacta ccgaattcaa tcggcgcatt tcttctttca ataaccctac
    32161 tgcattagcc aaactgtttc ctcttaatga attggaacat actagttcag caaaattact
    32221 tgtgtgatgc gcctcagtcg atttgactgg acgcatttca tataaacgga cagggactcc
    32281 tgcttgtgcg atctgccaag cagcttcact tccggcaagc cctgctccaa caacatttac
    32341 cattttcaat gaaagaacct cttttctatt atttttggat gtcttcttcg taatctccat
    32401 ttacacagac tacttgttta ccgcctttga ctttcttctc gaccagatat ccgtcacatt
    32461 ttggacaatt tctgcccact ggtttatccc aagaagtgaa ttcacattct gggaaacggg
    32521 aacaaccata gaatagtctg tttttcttag attttcgttc aatgacttgt ccttcatgac
    32581 atactgggca agtcacaccg atttctttta cgattggttt cgtattccga caatcaggaa
    32641 aattactgca agcataaaac ttcccatatt tccctaattt tatcaccatc ggatgtccac
    32701 agacatcaca atcgaatcct gctggttcat ctttgatttg aattttttcg attttttctt
    32761 ccgcattcgt cagttctttt tcaaacggtt tatagaaacg atcaaccact ttgacccacg
    32821 cttccgtgcc ttcttcaatt ttatccaaat ctccttccat ttctgctgtg aaatggatat
    32881 cgacgatttg agggaagaat tcacagatta atgaattgac aatttctcct agttcagtcg
    32941 gctcaaatct tttttgtgtc aattttacat agtagcgtct ttggatcgta tcaagtgttg
    33001 gtgcataagt ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc aacgtagcct
    33061 cactaaaacg tgctggtggt tgagtaaaat gttgttttgg ttcaatatca acagattgga
    33121 ctacatcgcc ttcttcaagt tctggaagaa tattttcttt ctcttctttg ccgtcatctc
    33181 taccttccac atacacttgc atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga
    33241 agatgacacc attttgttct aatgtcactt tcattgtatc taaaatagct ggtgtcatct
    33301 gactagccac aaaacgtgac cagatcaatg tataaagttt caattgatct ttatctaaat
    33361 attgtttgag ttcatctggt gtacgaagca cactcgttgg acgcacagct tcatgggcat
    33421 cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg tctagaaaat tcttttccat
    33481 acgtatcttc aataaatgct gcggcctctg cttttgctgt atcggcaatc cgggtagaat
    33541 cagtacgcat gtatgtaatc aagccgactg ttccttgttt acccaaagtg attccttcat
    33601 ataattgctg tgctaccatc atggtttttc ttgttcgaaa gtttaactta cgggcagctt
    33661 cttgttgcag gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt
    33721 ctactttttt aacttcgaag tctgttccat caattctagc agtgacttct tttacacttt
    33781 gtgcatctgg taatttcttt ttcttaccgt cgattcccca gaaatttgct ttgaatttct
    33841 tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc ttctggaaca aattctcgga
    33901 tctctttttc acggtcaata ataattttta aagcgacaga ttggacacgt cctgcactta
    33961 accctttctt gaccttacgc cagagaatag gactgatcga ataaccaacg attctgtcta
    34021 aggtacgacg agcttgctgt gcatctacta aatctacatc gatcgttctt ggttccttaa
    34081 aagctgcttt gactgcgtct tttgtgattt cattgaagac cacccgattt ttttctttca
    34141 gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc ttctccttct ctatccgggt
    34201 cacttgccaa gtaaactttt tgagcttttt ttgctgcggc tttcaggctt ttgatgacat
    34261 cgccttttcc gcgtatagaa atataatggg gttcataatt gttttcaaaa tcgataccca
    34321 ttttactttt cggtaaatct cttatatggc caacactggc cattacttta tagtttcgtc
    34381 caagatattt ctcaattgtc ttggcttttg caggtgattc tacaatcact aaatatttat
    34441 acgccattac tgaatggact cctttccatt gtcgtgttcc tactattaaa agaggattca
    34501 tacaaatcgt agctaatcat atccattcag aaaacgtttg tcaagaaaag caaatgaaaa
    34561 gtatggtgtt attctttaaa ttctgccaag atatcttcac ccgaaaccgt gcaaactgca
    34621 ccgtcttgga tcaaatgatg acatccggta gaatgttctg ttaaaatatt tcctggaaca
    34681 gaaaaaactg ttcttccaag atccaatgct tgctgtgctg ttatcagaga gccgcttttt
    34741 ttcttggctt caaagacaca tgttccttta gacaaagctg cgataatccg atttctcatt
    34801 ggaaaacaat gctttttcgg gccggattga ggaggatatt cactcaaaag aagatgatca
    34861 tttgcaatca cctcttgcaa gtcacgattt tcagcaggat agacttgctc tatccctgtt
    34921 ccaatgactg cgattgtccg tccaccatga gagattgctg cttggtgacc gtatgtatct
    34981 atccctttcg cgcagccact gacgatcaca tatccagctt ctatcactgg ttttagtaaa
    35041 taataaactg tttttttggc taaagcagaa gtgtgtcttc caccaacaac gcttaacatg
    35101 ttacaagaaa gtaaagatag atcaccatta taaaacagta caattggcgg ataggtcaag
    35161 tgaagcagct gagggggata tttgggatca aagcaagtga taaacgactg tttcgattgg
    35221 attttgcccc agttagcgtg aatttcctgc caacttttgc gaaacagctc ccggtgtttg
    35281 gttattttac ctatcttaat gatgtcttca aatccgattt ctttataagg atactgcatc
    35341 atccagttag caagtgccca ttttccttga ctactgattc cttttgtata agcaagtttc
    35401 aataaatttt cttctatttg atacatcata aaaccgcctt tcgtctctct ttctaataag
    35461 atacgtaaaa gcggctgttt tttattgtaa atattttttt acaggtgcaa aacttttacg
    35521 atggattggc gtaattcctt gtcgttcaag tccttctaag tgttcctttg ttccataacc
    35581 tgcattatgc gagaaaccat atccaggata aagcacgtca tattctttca tcaattggtc
    35641 tcgatatact ttagcaatga tacttgcagc agcaatcgac acagatcgtg catccccttt
    35701 gattattttt tcctgctgaa taggcaaatc caaggtcatc gcatcgatca acaaatagct
    35761 tggtacaatg gacaattttt ctactgcttg gatcatcgct aatttacttg cttcataaat
    35821 attcacatca tcgattgtct gctgatcaac gatcccgatc ccgattgcca gggctttttc
    35881 ttgaacttgt tgataaagaa gttctcgctt tttctctgat aattgtttgg agtcattcag
    35941 ccctaatatg atctcatttt tcggcaagat aactgcagct gccacgacag gtccagctaa
    36001 tggtcctctt cctacttcat caatgccggc aatccattgg tgaccttgtg cgtacccttg
    36061 cttttcaaag caaaacattt cttcatattt ttccttaatt aatgcttcct ttttcaatcg
    36121 tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt ggatcatttc ttaatgattc
    36181 tagtgtagga tcatctattc tctgtatgct tttcaatcgt tctttgatac tttggatgga
    36241 ttcagtcatc acttttttgc tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt
    36301 ttccttgacg aatatcatgc acgactaatt cgctgccacg atcatagtct tctttatacc
    36361 cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa tttttcttct tgctcgattg
    36421 ataaaccata acgatcgctg attcttcctg aataatatcg ggcaaaaaag tctaatccgt
    36481 aaatcgctaa atcatctaaa tggacaagct ggtctttgat cgcgcctgtc agcgctagtt
    36541 ttttgccgat ttcttcatct tcaaatttgg gccataaaat ccctggtgtg tctaacaatt
    36601 ccaactcatt acctaaacga agccactgct gaccttttgt tacgccaggc ttatttcctg
    36661 tctgtgcaat tttcttgcct actagtcgat tcatcaacgt agatttccca acatttggaa
    36721 tacctaagac cattgcccga atagcgcgag gcttgatccc ccgagcagca tctcttgccc
    36781 gtttttcttt caatgcttct ttagccgcag gtactagacc tttaattcct ttactttctt
    36841 gtgccgtgat agctaatgca agatggcctt gtttacgaaa atattcttgc cacagctttg
    36901 tctgttgtgg atctgccaga tccgctttat tcaataaaac aaggcgaggt ttttgctgaa
    36961 tgatctgatc tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa
    37021 atacgatgtc cacatatttc attttttcag aaacttctcg tcgggctttt gccatatgtc
    37081 ccggaaacca ttggatggtc attgttctct ccattccttt cataccggaa tattccccgg
    37141 ctttacttgt ttttttcttt ttcaatataa attcactttt ttattataaa gaaattccat
    37201 ctattagtta agtgaaagtg aaccagtcct cattctatca catcttcact tttttgatga
    37261 tgcaaatttt tattttggtt tctatatatg aatctaaatt tttgacagcg tttcctaaat
    37321 aagagatgat agacgaacaa aacagaaagg agacgaaaca aatgaatagt atggtttttg
    37381 ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta
    37441 aaaaaaatga agaattttct actgaagaag ctggttcaat ggtatgggat gacacttttt
    37501 ggataatgct tctctctcat gatttttata aaaaatttat aaaagataaa gaaatagctg
    37561 ataataaaaa aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa
    37621 aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt ccacgaagta gatatgggaa
    37681 tgcctgaaga agaaatgttt tcattagaag tccaagatct agacggaaat acattagaac
    37741 ccgtatggat gaagatgtaa gcagataact aatctgacgt tttttataac aaaaaaacaa
    37801 aggcggatga tacgataagt attctccgcc tctatttttt ttattcgtac aatctctttt
    37861 ttacagttta taaaagcagt cttctcaccc tttttttaat tgttagatgc cagtgtatgt
    37921 aaatcaccaa tgtgtcatga cacattggtg attttatggt atccttttat tattaactga
    37981 caaaggagag aatattttga acactttgat tgcaaaagac gtttttagat tgcccgaaat
    38041 gaatgcaaga ttgttgtcga cctctgctct tttattagcc cttgatgtca tcttatacaa
    38101 gatagcgatt ggtcctgctt attttcaaat cacattcgga tttttaagta tggcgcttat
    38161 gggttatcta tatggaccga tctgggctgg tttgcttgaa atgttgtcga acactttaaa
    38221 tttcacgata tttggttcag gaacgtttca agctgctttt cttatcacgg ctttccttgg
    38281 tggatttatt aatggcttat ttttatatca taaaaagata aacctgcctt ccgtcatcgc
    38341 tgcccaatta ttgattatga tcccagtcag cctttttatc aattcttggc tgatgtctgt
    38401 tttgtttggt gcagatttca aagtaatttt tctaggtaga ttcatcagga atatgatcca
    38461 aatccctatt caaataatgg ttgtttattc atttatcaaa actttagagg ctagaaaagt
    38521 gtttaaacga gctgtttgat tttacgtact aaggtagtaa actgattttg gattaaacaa
    38581 taaagcgtgc agtctcaaca gactgcacgc tttattgttt ctcactggat ccattttatt
    38641 tcatcaagtg gataatagac aaatcttgct ttacctagaa tatcagtttt attgatcgtg
    38701 ccaaacgaac gactatcttt tgaaactctt cgattatctc caagtacaaa ataattatct
    38761 tccggaagct tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt ataggatgca
    38821 tttgcatgat ctgatttcag gtttttagtt agataaggtt ccttaactac tttcccattg
    38881 atcttcaatt gatcattttg gtagctgaca ttttctcctg gaagaccgat tactcgtttg
    38941 atataaatcg tgccatctgc taattgaaaa acaacaatat caaatcgtct gatctccgaa
    39001 aatttttcca tcacaaccat atcaccttgc ttcaacacgt tttccattga attgccttcc
    39061 actggtaccg ggatgaataa aaatccccgc aacatgaacg caaggaatac agaaacaagt
    39121 aagtatttga atacaagcca gaagcgatta atatagcgtt gtttttttgt caatctctat
    39181 caatccatcc ctcttttttt cattctttct ttattataag aaaaatatca aaaaaatgcg
    39241 actagatagc tgaaataaaa ataattcttt caattaggct attggacaaa aaactttttt
    39301 gatcataaag tacaaaacta tctcttatat gtcatgatta ttttctaaat atttttccca
    39361 tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca gataattaga
    39421 aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg cttctgtagc
    39481 gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa
    39541 gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg gcgtgcatgg
    39601 tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc ttctgctaga
    39661 taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg tccactttca
    39721 gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc
    39781 accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt tggatcagtt
    39841 tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc atagataatc
    39901 tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt cggatctggt
    39961 acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt gtcgaaaggc
    40021 tggatcatac accagaagca gcctcctgca aatattgctc gttccattat gattccccct
    40081 ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag tactgctggt
    40141 gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg gcaaatatag
    40201 actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat acatcccgtt
    40261 ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat tgacttcgat
    40321 ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga ttggcaaact
    40381 caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt ccatcacata
    40441 cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag taccattgag
    40501 tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt gatactcact
    40561 aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg ttaagacagg
    40621 atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc gattagcaaa
    40681 aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga taaggaaggc
    40741 gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc ttgttttcat
    40801 gtttattgat catcctcctt ctctaaccat tggttttgtg tctcgatcag ctgatctctc
    40861 actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc ttcttcatag
    40921 agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg atctgttcct
    40981 tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt gattgggata
    41041 aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca attgttaaca
    41101 attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta gatgcctaaa
    41161 atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc tgtgacattt
    41221 tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaagccgaa taaattattt
    41281 tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc agctgttttg
    41341 atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc acttcgttca
    41401 ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc ttttagatca
    41461 tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc ttcagtcagc
    41521 gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc tctcacagta
    41581 gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc tgcatagatg
    41641 atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt
    41701 ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca aaaaggtagt
    41761 ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga gaataagctg
    41821 caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat acatgattgc
    41881 aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta cagggatatg
    41941 catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt cttttgcatc
    42001 cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat ttttcaaatt
    42061 attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga ttaattcaga
    42121 atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac cagttgttcg
    42181 gctgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt ataattttgt
    42241 attgtcttgt acttttttga cttcttcaag gattttctct actgttttac cttcttttgc
    42301 taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat cactgtcgat
    42361 aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta ctgttccact
    42421 caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta gctcatcata
    42481 catcgagatg aattctccaa ttggtggttg gcttgttttg ggcagatttt ttgctttcgc
    42541 catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata ctgttccgtc
    42601 gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat ttttttccat
    42661 cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc tctctttctt
    42721 taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt ttagtataac
    42781 agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc atggaaaaaa
    42841 cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa
    42901 ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc
    42961 attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt
    43021 taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat
    43081 tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga
    43141 gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg
    43201 gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga
    43261 tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc
    43321 ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat
    43381 tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt
    43441 taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa aaataaagct
    43501 atggatggga cagctctaaa taacagctag tctatccata gcttttttat atcgtcaatt
    43561 cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat tgatgcggat
    43621 aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa tcagaccagt
    43681 caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta cggtataata
    43741 tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg aaaaagatac
    43801 cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca tgcacgcctt
    43861 ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc aacggacggc
    43921 tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct gtcatttgtt
    43981 tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc
    44041 cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg attctaaaca
    44101 gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat atcttccatc
    44161 tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt
    44221 tctcggctca attgttcttt catttgttcg atatgattga tataaagatg agcatctcct
    44281 aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc
    44341 aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact gcgttgatac
    44401 aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg acaaggaggc
    44461 aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct tctagaatca
    44521 ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc accgtctttt
    44581 gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta tgtttcagca
    44641 aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt atattcttca
    44701 ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg tccattatag
    44761 tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt tttattttgt
    44821 aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct tttgatcaat
    44881 ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt
    44941 tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg atgcccttct
    45001 tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc tcctaatctt
    45061 ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt gcttctgttt
    45121 cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga tgattcatct
    45181 cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca atcgtttccc
    45241 attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg ctctgtattt
    45301 ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa taactggaca
    45361 tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac ttatctgcta
    45421 ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga aaacttctaa
    45481 aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt tcatccaata
    45541 ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc
    45601 ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat cgttcaagta
    45661 attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca gcttcttgta
    45721 agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta taatacgcaa
    45781 gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg ccagctagaa
    45841 tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt ctttcccgag
    45901 attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg taactgccat
    45961 ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc tctaaatcat
    46021 cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg atccgtgctt
    46081 gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc tgtttataaa
    46141 gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct ttttctaaga
    46201 cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt tcgaagattc
    46261 ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt gcgccacggt
    46321 attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg tttgttggtt
    46381 cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta actcgttttt
    46441 tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct aatttttgca
    46501 aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt tgttcttctg
    46561 ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt tcgatcaatg
    46621 cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag actgcttgga
    46681 gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata cggtaatcag
    46741 ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca ataatattta
    46801 agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga
    46861 ccaaaaaaga aatctggtca aacaaatctt tttcaccata agtttttttg aggtcagtta
    46921 cttttaattc tttcaacatg ttccctcctt atcttggata ttgtatcata aaaatagtga
    46981 acttcaggaa aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc
    47041 gattcgatcc tgttctgata tgttttgttt tttataaaac ttgaaaattt ttaccttttt
    47101 attttattgg actcgttttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat
    47161 cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg
    47221 cagaaaacgt cgtgattttt tcagcaactt cgtattcatc aatgattttt ctttcaagat
    47281 aataacctct tagcattttt ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa
    47341 tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa
    47401 ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat
    47461 ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat
    47521 cagcatatct tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga
    47581 caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc
    47641 gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac
    47701 tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt
    47761 gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt
    47821 ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt
    47881 ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg
    47941 cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga
    48001 tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg
    48061 aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta
    48121 agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa
    48181 aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg
    48241 ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca
    48301 attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg
    48361 tactgtggga caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag
    48421 cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca
    48481 atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg
    48541 ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga
    48601 tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag
    48661 atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca
    48721 aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa
    48781 gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag
    48841 caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa
    48901 tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat
    48961 ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag
    49021 gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt
    49081 ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg
    49141 ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat
    49201 tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt
    49261 caacctgtgt ttggttcccc tctaattcac actgtacacg ttttgactcc aaacgaagca
    49321 gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac
    49381 aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt
    49441 tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta
    49501 tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga
    49561 aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa
    49621 acttaagaaa gataaaagtt tctgacaaaa ggatgtttac tttcccattc ctgcctttct
    49681 tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat
    49741 tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac
    49801 agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca
    49861 cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat
    49921 tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca
    49981 ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga
    50041 ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct
    50101 tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca
    50161 ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg
    50221 ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc
    50281 attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt
    50341 tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta
    50401 gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa
    50461 aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct
    50521 aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt
    50581 ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct
    50641 acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca
    50701 tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt
    50761 agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc
    50821 aaaaaggaac gaatctcatt ttcttttaga tccagttggc tgatcaataa tgcccatgct
    50881 tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct
    50941 tcttctttca aaccgggaca gcaaatgtag cactctgttt caataaatgc atggattccc
    51001 gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat
    51061 attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg
    51121 aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac
    51181 cgctcatgag gattgcctac agctcgcaag atctgttctt ctagatcttg cagaccatcg
    51241 aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt
    51301 cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct acggtagtcc
    51361 tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc
    51421 aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct
    51481 tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca
    51541 cgaacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact
    51601 ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct
    51661 gcgtgtttct tgtcctgaga taaagactca gttgcatggt gactgtttga caagtccgtt
    51721 tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga
    51781 ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata
    51841 ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc
    51901 gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt
    51961 gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc
    52021 accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt
    52081 ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa
    52141 gagcaattga gaaaatcgaa tttccatgct acatttagga aggaatttct aaatggaaat
    52201 tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag
    52261 aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca
    52321 tcgctaatca tttagcagaa gggggagtct cagggatcac cttgatctta agagctttat
    52381 ttggtattga tccagcttat acgactttat taatcaacat cccattgatt ttactaggag
    52441 gaaagatcct cggtaaacgt tccttggcct atactgttct aggtacactt tctttgtcat
    52501 tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga
    52561 tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg
    52621 gtggaacaac aggcggcagt gatattatcg ctcggatttt agaacaaaat tacggcatca
    52681 ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata
    52741 tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg
    52801 attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg
    52861 aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttc ctttctggag
    52921 aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat
    52981 taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca
    53041 atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga
    53101 aaaaactata agaaaaaaga caaagatagg ctatgaggaa agattccaca tccagctgac
    53161 ctttgtcttt tttagtacta tactattttt aactaattgt tatgttcact tacccggtta
    53221 cttttctttt gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa
    53281 gcatccctat ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt
    53341 gcctcttctt ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact
    53401 gaagaatccc aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc
    53461 caagcatttt cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca
    53521 tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc
    53581 ccttcaacag atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca
    53641 aagtggacat tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact
    53701 tctcttcggc taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt
    53761 acatcaatta gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca
    53821 tatctcctgg ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc
    53881 cttcttctct taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat
    53941 gataagcctg ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat
    54001 gggctaggtt ccattctgca taaggattag cagtttcttc catctgattg atcacttgta
    54061 tcacatcttc atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat
    54121 cttgtttgtc tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat
    54181 gcagacggaa agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt
    54241 tgattccttc ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt
    54301 gtcctagata tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat
    54361 agttgattgc ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc
    54421 gatcatctgt cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt
    54481 ctccttgcat acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat
    54541 ttatcccgct tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg
    54601 aaaaatacaa ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg
    54661 cttctttcaa ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta
    54721 tcgccaataa gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa
    54781 aagctaagtc gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt
    54841 cattttctgg gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc
    54901 caatagccag caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg
    54961 ctttttctaa aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt
    55021 tttcgctatt tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc
    55081 gtcattgttc tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt
    55141 ttttctaaaa caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac
    55201 tccatctttg aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca
    55261 aaaaagtttt ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac
    55321 atcattccta aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa
    55381 aaagcttgtt caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa
    55441 tagatcattt cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga
    55501 tagtttttat acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa
    55561 gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg
    55621 atgcgttctg gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc
    55681 cgctgcggtg ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat
    55741 gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca
    55801 aatccgatga taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc
    55861 tttaattctg ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct
    55921 tgatttgcaa ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt
    55981 aaaaataaga ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg
    56041 taaaacactt tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct
    56101 cttcttattt ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca
    56161 cctcataact tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca
    56221 tacggattcg gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa
    56281 gcacttgtag ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt
    56341 aatgctttac ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca
    56401 gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg
    56461 attaattgaa ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct
    56521 actgctgcag ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat
    56581 tctgctttgt ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca
    56641 tttagttaaa actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat
    56701 attctgttat cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca
    56761 tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa
    56821 cgtcgatatg gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt
    56881 caaaagtaaa tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca
    56941 attcctcttc attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat
    57001 aaaaaatctt caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca
    57061 taatgatatc attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt
    57121 cgatcatttc aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg
    57181 gagcgtaatc aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat
    57241 tggtttcttt ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt
    57301 catgggcgta acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta
    57361 ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt
    57421 cagtattttc gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt
    57481 tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat
    57541 ccgaaacaat tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat
    57601 ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag
    57661 agaaatactt cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa
    57721 caggataagg atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg
    57781 gattatctac tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg
    57841 caaccatctc atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat
    57901 ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc
    57961 caagatcaat tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg
    58021 cataaatacg atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag
    58081 cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg
    58141 gatttgccat attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat
    58201 tgccaacttt caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata
    58261 ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga
    58321 tacaaaatca attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac
    58381 gcatcaccta aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac
    58441 tcaacatttt ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat
    58501 gcaatacgat gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca
    58561 tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc
    58621 ccttctactc cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt
    58681 ccgtctaata ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt
    58741 gtgtctttga ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca
    58801 tttacatcat cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc
    58861 tctgagacat ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta
    58921 agacgtgcaa cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt
    58981 ttcttttctt ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct
    59041 gatggttcga tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct
    59101 gcaacaaaat gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg
    59161 actaaaccgc ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa
    59221 tctctttcga tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag
    59281 ctgccgtttt ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg
    59341 acttttacta cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg
    59401 actccttcaa ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa
    59461 acttcgccct taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc
    59521 attgactggt tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc
    59581 aacattctgc cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa
    59641 gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa
    59701 aaagaagata accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg
    59761 atttgccagt cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc
    59821 ttgttgaatc ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg
    59881 gaattccttt ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta
    59941 ctaaaaagat tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca
    60001 tgacaacgcc accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt
    60061 ttccatgtgc agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag
    60121 tcacatcttt tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag
    60181 gatacttttt gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa
    60241 tagctaaata agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt
    60301 cttttgctaa tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa
    60361 tactgatttg tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct
    60421 attattattt aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc
    60481 cattcaattc aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac
    60541 cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt
    60601 ctgctgcatt gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg
    60661 ttgtctctgc tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt
    60721 cttccgcgat tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac
    60781 ttgtagaact ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt
    60841 tgcttgatcc atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca
    60901 gtaaaatcaa aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac
    60961 ttcttgatga aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt
    61021 cgttattttc tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct
    61081 atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt
    61141 ttttttaaat aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag
    61201 cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt
    61261 ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt
    61321 aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg
    61381 ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag
    61441 ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg
    61501 ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag
    61561 aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc
    61621 tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat
    61681 accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag
    61741 ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct
    61801 gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc
    61861 ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa
    61921 aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag
    61981 tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac
    62041 cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac
    62101 tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt
    62161 cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata
    62221 ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc
    62281 agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag
    62341 cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag
    62401 gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg
    62461 aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt
    62521 tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc
    62581 cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct
    62641 tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat
    62701 caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga
    62761 ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc
    62821 tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc
    62881 gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg
    62941 actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata
    63001 attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac
    63061 tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt
    63121 ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg
    63181 ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc
    63241 tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag
    63301 cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa
    63361 ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa
    63421 acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga
    63481 gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa
    63541 caatcatatt attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt
    63601 acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta
    63661 tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat
    63721 gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt
    63781 attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa
    63841 cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag
    63901 attcatttgc tatctatagt taattgacta gttttttcac ggaccctata ggacccattt
    63961 atattatttc aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc
    64021 tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc
    64081 atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc
    64141 ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac
    64201 agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt
    64261 taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga
    64321 atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt
    64381 tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt
    64441 tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa
    64501 aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc
    64561 tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg
    64621 ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag
    64681 tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt
    64741 aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa
    64801 taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta
    64861 gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat actacttaat
    64921 tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat
    64981 gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc
    65041 atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt
    65101 ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct
    65161 gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct
    65221 acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg
    65281 ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact
    65341 aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga
    65401 cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg
    65461 acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact
    65521 tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca
    65581 gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt
    65641 tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct
    65701 ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa
    65761 cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc
    65821 gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata
    65881 gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata
    65941 tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct
    66001 tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa
    66061 gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg
    66121 gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc
    66181 acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc
    66241 cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa
    66301 tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac
    66361 gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat
    66421 ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata
    66481 tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta
    66541 cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt
    66601 gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga
    66661 ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat
    66721 agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc
    66781 cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag
    66841 caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga
    66901 caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata
    66961 acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg
    67021 tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg
    67081 cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca
    67141 atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat
    67201 cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt
    67261 cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca
    67321 tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc
    67381 ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga
    67441 ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat
    67501 agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca
    67561 tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc
    67621 cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga
    67681 agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta
    67741 gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc
    67801 gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta
    67861 taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag
    67921 gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag
    67981 agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata
    68041 acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt
    68101 ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa
    68161 cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat
    68221 gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt
    68281 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
    68341 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
    68401 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
    68461 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
    68521 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
    68581 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
    68641 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
    68701 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
    68761 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
    68821 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
    68881 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
    68941 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
    69001 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
    69061 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
    69121 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
    69181 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
    69241 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
    69301 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
    69361 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
    69421 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
    69481 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
    69541 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
    69601 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
    69661 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
    69721 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
    69781 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
    69841 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
    69901 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
    69961 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
    70021 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
    70081 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
    70141 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
    70201 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
    70261 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
    70321 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
    70381 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
    70441 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
    70501 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
    70561 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
    70621 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
    70681 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
    70741 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
    70801 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
    70861 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
    70921 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
    70981 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
    71041 tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt
    71101 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
    71161 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
    71221 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
    71281 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
    71341 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
    71401 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
    71461 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
    71521 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
    71581 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
    71641 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
    71701 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
    71761 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
    71821 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
    71881 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
    71941 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
    72001 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
    72061 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
    72121 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
    72181 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
    72241 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
    72301 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
    72361 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
    72421 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
    72481 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
    72541 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
    72601 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
    72661 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata
    72721 gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg
    72781 ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc
    72841 gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata
    72901 acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc
    72961 aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa
    73021 gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc
    73081 tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc
    73141 cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa
    73201 aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg
    73261 gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct
    73321 tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc
    73381 atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct
    73441 aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat
    73501 gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct
    73561 agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt
    73621 aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc
    73681 atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc
    73741 caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc
    73801 catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga
    73861 ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa
    73921 tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg
    73981 taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca
    74041 aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct
    74101 gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct
    74161 taatacattt ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt
    74221 tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa
    74281 cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt
    74341 cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct
    74401 ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc
    74461 ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg
    74521 atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat
    74581 gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag
    74641 gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg
    74701 gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc
    74761 gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta
    74821 tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct
    74881 actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct
    74941 ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct
    75001 tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg
    75061 atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt
    75121 gctatgaacc actcattcac tttttggata tctcggatct tagattggat atcttggtaa
    75181 gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc attttttagt
    75241 tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa
    75301 aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct
    75361 acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga
    75421 agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct
    75481 ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt
    75541 attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct
    75601 tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc actatttatt
    75661 tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc
    75721 ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct
    75781 gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta
    75841 tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt
    75901 ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt
    75961 gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt
    76021 tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct
    76081 tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga
    76141 tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat
    76201 tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga
    76261 aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt
    76321 gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta
    76381 ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct
    76441 cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct
    76501 tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt
    76561 gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt
    76621 ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa
    76681 ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca
    76741 ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt
    76801 aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct
    76861 tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa
    76921 tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc
    76981 gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc accgatttct
    77041 tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta
    77101 ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct
    77161 cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg
    77221 tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg
    77281 aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct
    77341 tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact
    77401 tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg
    77461 tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt
    77521 ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata
    77581 cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt
    77641 gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg
    77701 atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa
    77761 ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa
    77821 tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt
    77881 tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga
    77941 tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc
    78001 ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg
    78061 agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt
    78121 ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct
    78181 gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa
    78241 cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat
    78301 cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata
    78361 gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga
    78421 ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt
    78481 taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat
    78541 acttacggag atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta
    78601 atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt
    78661 ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag
    78721 ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa
    78781 tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg
    78841 ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca
    78901 agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac
    78961 cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag
    79021 acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa
    79081 ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt
    79141 tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct aaagctggct
    79201 gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag
    79261 caatcagttt ccccgtagca gattcaggat tttggacagc tatagtacgc tgacgccatt
    79321 gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta
    79381 gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat
    79441 gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac
    79501 acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa
    79561 cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt
    79621 tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa
    79681 ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt
    79741 ttgattgtat catatttcat tgctttttca agtataacac atttctatct cattaagcat
    79801 caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga
    79861 agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag
    79921 tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt
    79981 actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa
    80041 ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat
    80101 gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa
    80161 gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa
    80221 gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg
    80281 ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc
    80341 gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca
    80401 aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga
    80461 cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa
    80521 acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt
    80581 accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct
    80641 tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt
    80701 aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc
    80761 agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga
    80821 tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc
    80881 tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga
    80941 ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc
    81001 agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc
    81061 acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta
    81121 tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga
    81181 tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc
    81241 aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt
    81301 cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa
    81361 ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa
    81421 gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa
    81481 ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga
    81541 gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca
    81601 gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg
    81661 acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata
    81721 acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc
    81781 agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg
    81841 gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata
    81901 acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac
    81961 agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat
    82021 atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt
    82081 ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa
    82141 ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt
    82201 ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt
    82261 ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa
    82321 acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac
    82381 cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac
    82441 tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat
    82501 ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag
    82561 tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt
    82621 agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca
    82681 tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa
    82741 ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt
    82801 ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa
    82861 tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc
    82921 tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt
    82981 aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc
    83041 accgctgact cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa
    83101 catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac
    83161 tatttgcgca ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt
    83221 acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat
    83281 aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc
    83341 gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag
    83401 aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat
    83461 aatcttt
//
LOCUS       DACCQP010000005        67949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCQP010000005 DACCQP010000000
VERSION     DACCQP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.005"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..109
                     /locus_tag="IVO87_000398"
     CDS             <1..109
                     /locus_tag="IVO87_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7448627.1"
                     /translation="YIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            237..1451
                     /locus_tag="IVO87_000399"
     CDS             237..1451
                     /locus_tag="IVO87_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7448628.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2161..2349)
                     /locus_tag="IVO87_000400"
     CDS             complement(2161..2349)
                     /locus_tag="IVO87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448629.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2440..2649
                     /locus_tag="IVO87_000401"
     CDS             2440..2649
                     /locus_tag="IVO87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP7448630.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2761..4374
                     /locus_tag="IVO87_000402"
     CDS             2761..4374
                     /locus_tag="IVO87_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP7448631.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4409..5620
                     /locus_tag="IVO87_000403"
                     /pseudo
     CDS             4409..5620
                     /locus_tag="IVO87_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5643..6569
                     /gene="glsA"
                     /locus_tag="IVO87_000404"
     CDS             5643..6569
                     /gene="glsA"
                     /locus_tag="IVO87_000404"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7448632.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6777..8237
                     /locus_tag="IVO87_000405"
     CDS             6777..8237
                     /locus_tag="IVO87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448633.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8458..10221
                     /locus_tag="IVO87_000406"
     CDS             8458..10221
                     /locus_tag="IVO87_000406"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7448634.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10305..10763
                     /locus_tag="IVO87_000407"
     CDS             10305..10763
                     /locus_tag="IVO87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7448635.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10872..11555
                     /locus_tag="IVO87_000408"
     CDS             10872..11555
                     /locus_tag="IVO87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7448636.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11702..12316
                     /locus_tag="IVO87_000409"
     CDS             11702..12316
                     /locus_tag="IVO87_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7448637.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12332..12538
                     /locus_tag="IVO87_000410"
     CDS             12332..12538
                     /locus_tag="IVO87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448638.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13079..14209
                     /locus_tag="IVO87_000411"
     CDS             13079..14209
                     /locus_tag="IVO87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7448639.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14301..15056
                     /locus_tag="IVO87_000412"
     CDS             14301..15056
                     /locus_tag="IVO87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7448640.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15114..17153
                     /locus_tag="IVO87_000413"
     CDS             15114..17153
                     /locus_tag="IVO87_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7448641.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17262..18413)
                     /locus_tag="IVO87_000414"
     CDS             complement(17262..18413)
                     /locus_tag="IVO87_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7448642.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18567..19097)
                     /locus_tag="IVO87_000415"
     CDS             complement(18567..19097)
                     /locus_tag="IVO87_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448643.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19196..19546)
                     /locus_tag="IVO87_000416"
     CDS             complement(19196..19546)
                     /locus_tag="IVO87_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7448644.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19703..21232)
                     /gene="ahpF"
                     /locus_tag="IVO87_000417"
     CDS             complement(19703..21232)
                     /gene="ahpF"
                     /locus_tag="IVO87_000417"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7448645.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21242..21805)
                     /gene="ahpC"
                     /locus_tag="IVO87_000418"
     CDS             complement(21242..21805)
                     /gene="ahpC"
                     /locus_tag="IVO87_000418"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7448646.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21949..22611)
                     /locus_tag="IVO87_000419"
     CDS             complement(21949..22611)
                     /locus_tag="IVO87_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7448647.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22795..24441
                     /locus_tag="IVO87_000420"
     CDS             22795..24441
                     /locus_tag="IVO87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7448648.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24432..25514
                     /gene="uxuA"
                     /locus_tag="IVO87_000421"
     CDS             24432..25514
                     /gene="uxuA"
                     /locus_tag="IVO87_000421"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7448649.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25622..27175)
                     /locus_tag="IVO87_000422"
                     /pseudo
     CDS             complement(25622..27175)
                     /locus_tag="IVO87_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            27323..28464
                     /locus_tag="IVO87_000423"
                     /pseudo
     CDS             27323..28464
                     /locus_tag="IVO87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(28590..29669)
                     /locus_tag="IVO87_000424"
     CDS             complement(28590..29669)
                     /locus_tag="IVO87_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7448650.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29772..30212)
                     /locus_tag="IVO87_000425"
     CDS             complement(29772..30212)
                     /locus_tag="IVO87_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7448651.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(30269..30679)
                     /locus_tag="IVO87_000426"
     CDS             complement(30269..30679)
                     /locus_tag="IVO87_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7448652.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30859..31293)
                     /locus_tag="IVO87_000427"
     CDS             complement(30859..31293)
                     /locus_tag="IVO87_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7448653.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(31393..32242)
                     /locus_tag="IVO87_000428"
                     /pseudo
     CDS             complement(31393..32242)
                     /locus_tag="IVO87_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
     gene            32631..34397
                     /locus_tag="IVO87_000429"
     CDS             32631..34397
                     /locus_tag="IVO87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7448654.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            34499..35758
                     /locus_tag="IVO87_000430"
     CDS             34499..35758
                     /locus_tag="IVO87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7448655.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35905..37206
                     /locus_tag="IVO87_000431"
     CDS             35905..37206
                     /locus_tag="IVO87_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448656.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            37209..38060
                     /locus_tag="IVO87_000432"
     CDS             37209..38060
                     /locus_tag="IVO87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448657.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38073..38888
                     /locus_tag="IVO87_000433"
     CDS             38073..38888
                     /locus_tag="IVO87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7448658.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39039..40205
                     /locus_tag="IVO87_000434"
     CDS             39039..40205
                     /locus_tag="IVO87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7448659.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            40435..41082
                     /locus_tag="IVO87_000435"
     CDS             40435..41082
                     /locus_tag="IVO87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7448660.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41166..42110
                     /locus_tag="IVO87_000436"
     CDS             41166..42110
                     /locus_tag="IVO87_000436"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7448661.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            42245..43696
                     /locus_tag="IVO87_000437"
     CDS             42245..43696
                     /locus_tag="IVO87_000437"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7448662.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            43717..44553
                     /locus_tag="IVO87_000438"
     CDS             43717..44553
                     /locus_tag="IVO87_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7448663.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            44553..45212
                     /locus_tag="IVO87_000439"
     CDS             44553..45212
                     /locus_tag="IVO87_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7448664.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            45338..46027
                     /locus_tag="IVO87_000440"
     CDS             45338..46027
                     /locus_tag="IVO87_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448665.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46033..46989
                     /locus_tag="IVO87_000441"
     CDS             46033..46989
                     /locus_tag="IVO87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7448666.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47033..47545
                     /locus_tag="IVO87_000442"
     CDS             47033..47545
                     /locus_tag="IVO87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7448667.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(47801..48349)
                     /locus_tag="IVO87_000443"
     CDS             complement(47801..48349)
                     /locus_tag="IVO87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7448668.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(48438..49619)
                     /locus_tag="IVO87_000444"
     CDS             complement(48438..49619)
                     /locus_tag="IVO87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7448669.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(49855..51363)
                     /locus_tag="IVO87_000445"
     CDS             complement(49855..51363)
                     /locus_tag="IVO87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7448670.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            51522..51713
                     /locus_tag="IVO87_000446"
     CDS             51522..51713
                     /locus_tag="IVO87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448671.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            51916..52833
                     /locus_tag="IVO87_000447"
     CDS             51916..52833
                     /locus_tag="IVO87_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7448672.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            52826..53899
                     /locus_tag="IVO87_000448"
     CDS             52826..53899
                     /locus_tag="IVO87_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448673.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54047..54565
                     /locus_tag="IVO87_000449"
     CDS             54047..54565
                     /locus_tag="IVO87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448674.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            54546..54938
                     /locus_tag="IVO87_000450"
     CDS             54546..54938
                     /locus_tag="IVO87_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7448675.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55014..55949
                     /locus_tag="IVO87_000451"
     CDS             55014..55949
                     /locus_tag="IVO87_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7448676.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56082..56861
                     /locus_tag="IVO87_000452"
     CDS             56082..56861
                     /locus_tag="IVO87_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7448677.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            56854..57171
                     /locus_tag="IVO87_000453"
     CDS             56854..57171
                     /locus_tag="IVO87_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7448678.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            57415..59025
                     /locus_tag="IVO87_000454"
     CDS             57415..59025
                     /locus_tag="IVO87_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7448679.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            59182..60030
                     /locus_tag="IVO87_000455"
     CDS             59182..60030
                     /locus_tag="IVO87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7448680.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            60261..60740
                     /locus_tag="IVO87_000456"
     CDS             60261..60740
                     /locus_tag="IVO87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448681.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            60773..63001
                     /locus_tag="IVO87_000457"
     CDS             60773..63001
                     /locus_tag="IVO87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7448682.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63130..64773
                     /locus_tag="IVO87_000458"
     CDS             63130..64773
                     /locus_tag="IVO87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7448683.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(64846..65391)
                     /locus_tag="IVO87_000459"
     CDS             complement(64846..65391)
                     /locus_tag="IVO87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7448684.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(65414..65899)
                     /locus_tag="IVO87_000460"
     CDS             complement(65414..65899)
                     /locus_tag="IVO87_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7448685.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(65889..66701)
                     /locus_tag="IVO87_000461"
     CDS             complement(65889..66701)
                     /locus_tag="IVO87_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7448686.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(66840..>67949)
                     /locus_tag="IVO87_000462"
     CDS             complement(66840..>67949)
                     /locus_tag="IVO87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7448687.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        22707 a        12055 c        13355 g        19832 t
ORIGIN      
        1 gtatattcct ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg
       61 gaatagtgat aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta
      121 tatccataca cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta
      181 atgacaggca attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga
      241 atgataaaaa ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa
      301 caaggcttgc tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa
      361 aacagccatt gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc
      421 cgcaagaacc actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg
      481 cattggcgat tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt
      541 atggaaaata cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca
      601 tgcaggagca tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat
      661 gttatctgct ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg
      721 gtggtgtgat gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc
      781 caaagttaaa ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag
      841 attacagcgg tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat
      901 atctcggttc aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt
      961 atgtcaagaa tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca
     1021 tgaagaacga acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac
     1081 gcaccgcttt ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc
     1141 caaaaagtca atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc
     1201 cgatacgctt aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac
     1261 aaagacaagt cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa
     1321 cgatactgaa agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac
     1381 aattagagaa atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg
     1441 gtatttttta atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc
     1501 ctatgtgaag tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact
     1561 tgcgtacaga tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa
     1621 atcggggctt gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg
     1681 aaattcgtga ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt
     1741 caaacaggca ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact
     1801 taacaattta aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg
     1861 gcagtaacgg tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca
     1921 tcaacttagc aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt
     1981 ttccatttaa acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa
     2041 tcaaccgttg attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg
     2101 attttggtta cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt
     2161 tcaatcgttc tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt
     2221 tgaaacaaat acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct
     2281 tgtaaaagat ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg
     2341 tacagccaca gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact
     2401 atgcagaggt ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt
     2461 tacagaaagg gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc
     2521 aagtgtggca ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa
     2581 aagggcaaaa caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca
     2641 atcgcataaa atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta
     2701 tactttactt gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca
     2761 atgaaacaac agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta
     2821 cagggcgaaa gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa
     2881 catcatttga atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac
     2941 agaccgagtt ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta
     3001 acgaaagacc tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg
     3061 tatttcccta gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa
     3121 ggcgaaagtg agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca
     3181 agccgtaaag tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct
     3241 tatgctccgt taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat
     3301 gaaacaaaat ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca
     3361 aaaatcacaa agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca
     3421 agatacggta cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg
     3481 acgattgctc atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac
     3541 aatatgcagt ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa
     3601 tggtttcgag tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg
     3661 caggagcaga taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca
     3721 gggcttgtca agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat
     3781 aaaacgcctt acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt
     3841 tctatgcact acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat
     3901 tgggctaagg cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc
     3961 aatgcagaga gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac
     4021 agacaaaatg agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag
     4081 ataacagagc gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg
     4141 gaacagcaga taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt
     4201 gctgaaaagt ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca
     4261 ttattaaatg ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac
     4321 gaaagaatac aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca
     4381 agagtaatat ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt
     4441 gggaaaacga ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta
     4501 cttgaccatg ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat
     4561 acaaaacaat aaatatctga aattggcaat cctattgatt attttctggg ctattttact
     4621 ctcacagtta aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg
     4681 tatcttgttg ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc
     4741 tccactacaa acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac
     4801 gctggttgtt tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca
     4861 tgcaaatgaa atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat
     4921 tctggcaatc attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca
     4981 tggtttgtct cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga
     5041 tccaaatctt gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg
     5101 agaaattgct tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg
     5161 attacttcct gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat
     5221 cgcaaatggg atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa
     5281 taatctttca tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt
     5341 tatgtttttc gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta
     5401 tcaggttcca ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc
     5461 actttttgct ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg
     5521 gatttatgaa acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat
     5581 ctacgcatgc cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc
     5641 aaatggaagt aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag
     5701 gacaagtggc ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg
     5761 cgatttatga tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg
     5821 ccatcgaaag catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag
     5881 aaaaagtctt tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga
     5941 atatggaaat caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg
     6001 caacaacctc gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat
     6061 ttatgaaaga gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg
     6121 aatcaagaac aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga
     6181 tcgaaggaga cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca
     6241 ccgctacagg catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga
     6301 atggcaaaca aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg
     6361 ctggtttgta tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg
     6421 gtgtaggcgg cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc
     6481 cggcactaga tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca
     6541 aagaactaga cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc
     6601 aaacggctca aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc
     6661 agtttctttt tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac
     6721 gatttacaga tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc
     6781 tggaaaatta catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg
     6841 aattccaaga aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga
     6901 tccttcacga tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa
     6961 gagagaagca taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac
     7021 agtttctgta cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat
     7081 tcacgagtac agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca
     7141 aaaaaatcgt attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta
     7201 tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag
     7261 gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag
     7321 agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa
     7381 tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa
     7441 gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa
     7501 ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt
     7561 ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg
     7621 ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg
     7681 acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg
     7741 cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc
     7801 tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa
     7861 gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac
     7921 aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt
     7981 tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt
     8041 tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt
     8101 tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa
     8161 tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa
     8221 aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt
     8281 ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt
     8341 tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat
     8401 aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg
     8461 agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct
     8521 gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc
     8581 gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat
     8641 tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt
     8701 cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc
     8761 ggatattcca accaacatga aagtaaacat gactatttta cagtggggca cacatctaca
     8821 tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat
     8881 gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac
     8941 aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt
     9001 gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa
     9061 agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac
     9121 gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta
     9181 cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa
     9241 tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga
     9301 gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct
     9361 cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa
     9421 aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct
     9481 tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa
     9541 cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta
     9601 gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct
     9661 tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc
     9721 tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca
     9781 catggtgtag aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca
     9841 gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa
     9901 gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg
     9961 atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc
    10021 acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt
    10081 gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct
    10141 gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg
    10201 cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt ttatactagt
    10261 atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag
    10321 aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt
    10381 tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa
    10441 agataaagac tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac
    10501 caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca
    10561 aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag
    10621 aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac
    10681 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
    10741 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
    10801 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
    10861 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
    10921 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
    10981 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
    11041 gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
    11101 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
    11161 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
    11221 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
    11281 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
    11341 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
    11401 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
    11461 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
    11521 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
    11581 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
    11641 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
    11701 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
    11761 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
    11821 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
    11881 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
    11941 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
    12001 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
    12061 catttcagta ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
    12121 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
    12181 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
    12241 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
    12301 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
    12361 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
    12421 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
    12481 ggaaatagaa atgccagatc aaacgattag cggattgttt aattacttta acaaataaat
    12541 atttattccg aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa
    12601 gaatcctggt gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt
    12661 ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa
    12721 tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg
    12781 aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa
    12841 cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata
    12901 gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct
    12961 gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct
    13021 gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggatttt
    13081 gggtccttat ttgactttgt ccatgatcgg tcttttagaa gtatacagtg ccagttctta
    13141 tcgtttattg caagccgatg aaaatacaaa aagtctatta ttgcgtcaac taattttcat
    13201 atttttgagt tggagcgtga tcttcttagc tcgttcagtc aaactacact atttacttca
    13261 ccctaaaata gcaggatacg gtttagcctt atcgattttc tttttagtat tagtaagaat
    13321 agggatattc ggtgtcactg tcaacggcgc acaacgttgg atctctctgt ttggcattca
    13381 attccagcct tctgaactgg caaatctttt tttgattttt tatttaagct ggttttttcg
    13441 tgacggaaat agtagcccaa aagatctaaa aaaaccattc ctgattacag taggtataac
    13501 ttttctgatt ttatttcagc caaagattgc tggagcattg atgatccttt cgattgcgtg
    13561 ggtcatattt tgggcagcgg cggttccatt taaaaaaggg atctatctaa tcgttacttt
    13621 ttctgcattg ctgattggag cagcaggcgg ggtattatat ttaggaaata aaggttggct
    13681 tccacagatg tttaatcatg cttatgaaag gatcgcgact ttaagagatt cttttatcga
    13741 tagtcatggt gcgggctatc aaatgaccca ttcgttttat gcactatata atggtggcat
    13801 atttgggcgg ggattaggaa atagtatcac aaaaaaaggg tatcttccag agacagaaac
    13861 agattttatt ttttcgatta ttacagaaga gcttgggcta atcggtgcat tgtgtgtgct
    13921 tttcttattg ttctcattat gcatgagaat cttttgttta agcagtcgct gtaaaaatca
    13981 gcaagctgga ttgtttcttt taggattcgg aactttgcta ttcgttcaga cgataatgaa
    14041 cgtgggaagt atcgcggggc tgatgccaat gactggtgtg ccattgccat ttgttagcta
    14101 cggaggaaca agttatctga tcctttcttt aggaatcgga atcacgttga atatttcttc
    14161 aaagatacag gccgaagagt tggcgctcta tcgacctgaa aaacaataaa gagaaactga
    14221 gaaactttca gtttgaatta tcataaagta caatctttta tttttcaagc gagaggttct
    14281 tttagaaaga ggaattcgca atgaaagtat ctcaaaaaat catattatct attttgttac
    14341 tgcttacatt agtagttggc tatttttcga ttgagtttat ccatggattt tcgtctgcaa
    14401 aacagagttc aactgtgaaa aaggtagagc cgaaaagtat ccctaccaca ctaaatgtgg
    14461 ctttgattgg ttcagatgct cgttcgaaag acgaaaacgg tcgctcggat tcacttctgg
    14521 ttgcacaata caaccagaaa acacaacaag caaaactgat ctctatcatg agagattcat
    14581 atgtcgatat tccaggttac ggaatgaata agataaatgc agcttactct tatgggggag
    14641 ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa
    14701 gtatcacatt tcaagatttt atcgattgcg ttaatgaact gtttcctggt ggagtcaaga
    14761 tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga gagcaagtga
    14821 tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa ggagacttcg
    14881 ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg aaagacgtaa
    14941 cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc caaacgaacc
    15001 ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata tgttagaata
    15061 aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa
    15121 gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca gtgatcttaa
    15181 tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc caagcagtag
    15241 aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga gattataaca
    15301 aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct gaaaaagaga
    15361 tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt cagatatcga
    15421 atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa gcaaagatga
    15481 atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac agaaatgatg
    15541 gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa ggaaatgaca
    15601 aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga aatggtgaac
    15661 cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa cttggagatg
    15721 gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta acagaagatg
    15781 ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc ccattgaaaa
    15841 tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa gtagacggca
    15901 gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga gatatcacag
    15961 cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt
    16021 tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta agcattacag
    16081 atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat ggcaaagata
    16141 tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt tgacagtcta ggaggaaaag
    16201 ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt gctagctctc
    16261 caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac aaagcttatg
    16321 aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct cctggatcta
    16381 cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag
    16441 tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct tatcaagcaa
    16501 cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc tattccgata
    16561 atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag
    16621 ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca gcacaaattt
    16681 ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat ggacagggcg
    16741 aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc aacaatggca
    16801 cacttgtcta tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca
    16861 gcgaaacagc agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg
    16921 gtacagcaca ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg
    16981 aaatcaaaga aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc
    17041 ttgataacca aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag
    17101 caaccaaacg agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg
    17161 ctttttaagt acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac
    17221 ggttttcacg atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt
    17281 tttgtaaaat atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact
    17341 aaaggtgaat agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc
    17401 aaagccattt ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa
    17461 aaaccggcaa tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc
    17521 gtcaatatag aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct
    17581 acactgacaa aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat
    17641 acttcttctt taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca
    17701 gagctaagcc caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg
    17761 atgataatag aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc
    17821 catttgacta ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt
    17881 aaagaagcat tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa
    17941 acgactaaaa tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc
    18001 actacgttta gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata
    18061 atcccaaaga aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca
    18121 aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt
    18181 ttcaaaagag ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc
    18241 tccgaaaagt catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc
    18301 aaaacaccaa caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt
    18361 gagccaatcg ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc
    18421 attcctttgt ataaaatata gcccgaactt agcataacat acattattga aaaaatagga
    18481 aaatatactc acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata
    18541 cggtctattt taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa
    18601 ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc
    18661 atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta
    18721 ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg
    18781 ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa
    18841 gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa
    18901 gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca
    18961 agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc
    19021 gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt
    19081 ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat
    19141 tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac
    19201 ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat
    19261 aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac
    19321 ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag
    19381 tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa
    19441 tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag
    19501 gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt
    19561 ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta
    19621 ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct
    19681 gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg
    19741 cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac
    19801 cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc
    19861 ttggattcat ttcgatcgtt ccatctagcc aatccgtatt agggagtaag ccgactagaa
    19921 tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga
    19981 gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga
    20041 cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga
    20101 attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag
    20161 aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg
    20221 ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc
    20281 agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc
    20341 caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt
    20401 cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt
    20461 aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat
    20521 cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg
    20581 ctggtccacc gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa
    20641 tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt
    20701 cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt
    20761 ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat
    20821 tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat
    20881 gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc
    20941 gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac
    21001 cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca
    21061 tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt
    21121 cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt
    21181 caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca
    21241 cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt
    21301 ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa
    21361 tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc
    21421 cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat
    21481 caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga
    21541 tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa
    21601 agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag
    21661 taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa
    21721 gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt
    21781 cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat
    21841 tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac
    21901 tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa
    21961 atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa
    22021 tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata
    22081 atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa
    22141 acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat
    22201 ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat
    22261 tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc
    22321 caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc
    22381 attttctgca gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata
    22441 cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc
    22501 aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat
    22561 aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc
    22621 tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg
    22681 tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat
    22741 tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt
    22801 gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct
    22861 cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc
    22921 ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta
    22981 ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg
    23041 gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc
    23101 gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa
    23161 cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct
    23221 attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg accgattgca
    23281 gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta
    23341 ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt
    23401 tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag
    23461 gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt
    23521 ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg
    23581 gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct
    23641 ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga
    23701 cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca
    23761 gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt
    23821 tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta
    23881 gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat
    23941 ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat
    24001 atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg
    24061 attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg
    24121 actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc
    24181 ccgagtccag acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt
    24241 acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt
    24301 tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag
    24361 atggtagcag gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga
    24421 ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct
    24481 tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg atggatatac
    24541 cagtaggaga ggtatggcct agggagagga tcaaagaact aaaagaagaa attgagtctc
    24601 gtgggttaac actaaaagta attgaaagcg tcaatgtgca tgatgccatt aaaattggat
    24661 taccttcccg tgagcagtat atcgaaaact ataagcagac aatccgaaac ttggcagaat
    24721 atggaataga agtcatctgc tataatttta tgcctgtatt tgactgggtg aaatctgacc
    24781 ttgactaccg cttagaagac ggctcatcca cgttagcttt tatatctgat gatattccag
    24841 atgatccaag agaaatcgtc gcgcgcatcg aacattccag caatgaattt ggattgccgg
    24901 gatgggaacc tgagcgctta gctcctatca aatccttgct tgaagcctat gcatcagtag
    24961 acgaagaaaa acttcgagaa aattttgcct attttctcca atcaatcatc ccaacatgtg
    25021 aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc gccatactcg atttttggat
    25081 tagctcgaat cgtaaagaat cgagaagatt tagattggtt gtgtcgcata gtagacagcc
    25141 cttataacgg aatcactctt tgtacaggga gtattgcaga agatccagaa aatgatgtat
    25201 atgggatttt agcagaattt tgccaaagag atcgaatccc atttgcacat gttcgaaata
    25261 ttaaattttt agctaatcga gatttctatg aggcaccaca taaatccgaa tacggttcat
    25321 tagacatgta ccgcatacta aaaacaatgt atgacaacgg atttaacgga tacatccgtc
    25381 ctgatcatgg aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc
    25441 gagcattagg tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa
    25501 gtaaagataa ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa
    25561 ttcttgatac ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg
    25621 tctaggaagt aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt
    25681 caaagatata ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc
    25741 cttttaaaat atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga
    25801 taacgatgtt gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa
    25861 tttgttctat cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac
    25921 caatcacaat tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca
    25981 tccattgcac attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga
    26041 aatccatata aaattgttca atggcggttt ggacttcttt caatacgcta tgttcttgat
    26101 aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa gttaagaaat ccattataaa
    26161 gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt ataaacaaat
    26221 caaatacagt acaaagggac tccaccattt ctcttgaata aagaacatga ggaacgtttg
    26281 ctttttttaa aagtttgtat tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag
    26341 aaagtcgagc taaaatattc actaagatgc gttgttcgcg acaaattggc agattaggtc
    26401 gcaaaatatc tacagagaga tcaataaaaa agtcaatata ttcatactct gtgaattctt
    26461 tttgtacgtc tttcacagaa gttgcaggaa aattctcttt acatattttt aatagaaaat
    26521 ataatttatc tatcataccg ctatttagtc ggtcaagtac ttctttttta aaaaaagtat
    26581 tcatcataaa agccactact tgcggatctt catcataaaa ttctgtagca gtacttgaaa
    26641 agatgttcca ataaatatct aatgctgcat attgaatttg tacctctttt ccagaaaaag
    26701 actttctgcc attattagaa aaggagatag acaaaccgta ctatgctaaa aatgcattaa
    26761 tctttttcat tttcgtatac aaataagagg cactatagtt tgtttgctga ctgaaactga
    26821 taatcgaatt tgtctgacag ctgaacaaag aagtcaagat ctgataactg actgattcgt
    26881 tgcagtatct tccaaataga cgataatagc agtaagccgg atcaattcct tcaaaattca
    26941 actttaaaaa aggtttttgc ttatcaatat gcagttctgt tagattgaga cgctcaaagt
    27001 cttgttctaa ttctttgatg tatagtaaaa aatttgattc agacatatgg aaatgatctg
    27061 ctaaatattg tgtagtaata aagaaacgat gttcagatag gaaaagaaaa atatctaatt
    27121 ttgtattgct ttttttcttc aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag
    27181 aggctccttt ttacttaaat tgttaaaact tgaaaatatt actgtttttt tcataaattt
    27241 agacaaaaaa acagtaaaca tataggtaga atctctacta tcttatttct tgcatacaaa
    27301 aaaatcaagg agtgagagag ttatgagata ttcaaccatc ttaatatcaa catttgcagc
    27361 cttaagctta tcgcttacta taaatcagac agtccacaca gaggagacag aaacagccaa
    27421 atggacagaa accaagtggg ccaacgtcaa tcgaattacc tttcaggatg aattttcatc
    27481 cttcaattca ggagtcgcaa aattaagaaa aggacaaaca gcgaccatca aagcaaaaat
    27541 tgactattcg ggagacttag cacctatact acaatcagct gttacatttg agaatgttga
    27601 tcccactttg tcgattggct atgacgagaa cactttgact atccaacaca agcaagcgat
    27661 atttaccttc accgttacat taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa
    27721 ggtatcagat ggactaccga acgatcatca tttaacgccg tatagtcagc ggcagacaat
    27781 tgagcaagat tcagccatcg actcaggagg agatcttgtt gaagaaccaa cagataaacc
    27841 agaaaatgaa aataagccag aagtaccgcc tacagaaaat ccagacggag aacagaaacc
    27901 ggaaatagaa cctggagaag aacccgatac agaaactcaa ccagagccag acaataaatc
    27961 aaaacctgaa ataacccctg gagaaaaacc agatgtaact ccagaaccag atacggattc
    28021 tggaaaccaa acagtaccgg aaacaaatcc agacacagat aatgaaactg aaaatccaga
    28081 aaaaccagag gtagatccag aagaaaaacc agatgtgact ccagaaccag atacggacgc
    28141 aagagaccaa ggaataccag aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa
    28201 agagagtaaa aaatcaaatc tagcaattct aaaaataaat gaagagcagc tgaataaaaa
    28261 atcacgaata tttgatagcg cccaatcagc tgaaacctta aaaagtagca aggatacaac
    28321 ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc acaaaacaaa
    28381 gttatactgt ggagcggaat cattctatta agtatagcaa caatgctttc tgcaaaaaga
    28441 tttaaacaaa acagatctct ctaacatttg aataatgtct gtgaacgcat ttttcaaata
    28501 aaaagaaata tccagaaaat gaccccaaaa taccagattg aaatctgata tttggggtcg
    28561 ttcatacttt atctttcttc atatttattt taagggatat ctgtttcttt tggaagatgg
    28621 ttcgggatct ttttcacttt caatacatct aaaaagaagg taaagatcgg aatgccgaca
    28681 atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa
    28741 atcggcagtt ctgttttgct ggacatcaac ttcggattca gcacataaga ctcaattaga
    28801 tggacaatca aaatcgtaat caggatataa acaacatctc ggatgcctcc ttgggaataa
    28861 gctatgaaag atagaggaat acaagaaatg ataacacctg ctactgggat cagacttaaa
    28921 atgaaaatca tgatcgctaa actcagcagc tgatgaaagc caaatacagc caaggcgatt
    28981 gtagtgagca acgtatttaa aagagcgaca acaaattgag cttctaatac caaaccaaat
    29041 gtattgacaa acttatcggc aaaatagtaa atatcttgaa agaaccaaga aaattcactt
    29101 tttaaaaaca atcttgagaa tacaattgtt tttttctttt caatcataaa aaagaaactt
    29161 aagatgaatg acatagcaaa cgccactgcc atttttccta tatcctgtaa atagccaagc
    29221 gcaatcgaag cgccattttg aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg
    29281 atatattgta aaacaggatc tgcatctttt ggctgatgct cgtaaaaatt aataacggac
    29341 tggatcatgt caaatgtttg atgaataagc atcgggatat actttgtgaa tgccagatag
    29401 ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa cacctgtagg gattcggaca
    29461 aatcgctgaa tgatttgaac aagatgaatc atcaaataag taaagataaa tgtgagcaaa
    29521 atggttgtaa tcatacttct aactaagaaa agaacaaaaa cgacaaataa taaaacagtg
    29581 agacggcgta ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc
    29641 aaataacaaa aaataaacac gttattcatt atatgatgga atatgaaaat aagaaagagc
    29701 aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc
    29761 atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc
    29821 agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt
    29881 ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag cagtcagcgt aacatttacg
    29941 attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt
    30001 agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa
    30061 gtctttttct tttcccataa aactaacatc acaagatact gggtataggt aagattcagc
    30121 ggcttgagga ggggagtgta gtgtctaatg atttctttag aggcggcata taaaggaaaa
    30181 catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt
    30241 gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta
    30301 tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt
    30361 gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc
    30421 tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat
    30481 ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa
    30541 acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt
    30601 cgaatgtacg atcgggatta tgcacttctc cagagcgtcc gcctgtgttg atcattttcg
    30661 tagaatacat ttttttcata aaattaccac ctttaattat attttataca attgaatttt
    30721 acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca
    30781 catgaaacag acgcgctttc tcgaatagca aacatataca aaaaatatca gagacaaaaa
    30841 ataatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaaat
    30901 tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga
    30961 gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg
    31021 taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt
    31081 tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa
    31141 tctcgtacca tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc
    31201 aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt
    31261 taatacaaaa tcgatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa
    31321 cagttactag attcttttat tataccagct aaagaaaacg cattcaaaaa aatagcggat
    31381 ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac
    31441 gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga
    31501 aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc
    31561 ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc
    31621 ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat
    31681 ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat
    31741 tccaaaaata aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
    31801 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
    31861 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
    31921 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
    31981 aaccaagctt ccgaaaagta ctttaaaaaa agggtttttt acctaaaaat agaagagcta
    32041 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
    32101 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    32161 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    32221 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
    32281 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    32341 aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt
    32401 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    32461 aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    32521 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt
    32581 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    32641 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    32701 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc
    32761 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    32821 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt
    32881 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    32941 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    33001 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    33061 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    33121 gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc
    33181 atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag
    33241 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    33301 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    33361 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    33421 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    33481 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    33541 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    33601 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    33661 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    33721 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    33781 atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa
    33841 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    33901 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    33961 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    34021 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    34081 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    34141 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    34201 taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca
    34261 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    34321 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    34381 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    34441 tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat
    34501 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    34561 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    34621 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    34681 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    34741 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    34801 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    34861 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    34921 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    34981 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    35041 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    35101 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    35161 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    35221 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    35281 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    35341 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    35401 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    35461 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    35521 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    35581 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    35641 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    35701 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    35761 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    35821 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    35881 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    35941 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    36001 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    36061 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    36121 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    36181 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    36241 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    36301 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    36361 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    36421 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    36481 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    36541 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    36601 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    36661 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    36721 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    36781 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    36841 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    36901 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    36961 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt
    37021 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    37081 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    37141 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    37201 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    37261 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    37321 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    37381 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    37441 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    37501 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    37561 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    37621 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    37681 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    37741 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    37801 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    37861 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    37921 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    37981 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    38041 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    38101 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    38161 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    38221 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    38281 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    38341 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    38401 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    38461 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    38521 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    38581 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    38641 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    38701 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    38761 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    38821 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    38881 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    38941 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    39001 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    39061 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    39121 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    39181 tcccaatgaa agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc
    39241 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    39301 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    39361 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    39421 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    39481 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    39541 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    39601 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    39661 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    39721 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    39781 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    39841 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    39901 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    39961 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    40021 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    40081 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    40141 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    40201 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    40261 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    40321 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    40381 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    40441 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    40501 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    40561 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    40621 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    40681 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    40741 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    40801 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    40861 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    40921 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    40981 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    41041 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    41101 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    41161 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    41221 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    41281 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    41341 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    41401 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    41461 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    41521 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    41581 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    41641 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    41701 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    41761 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    41821 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    41881 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    41941 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag
    42001 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    42061 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    42121 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    42181 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    42241 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    42301 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    42361 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    42421 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    42481 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    42541 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    42601 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    42661 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    42721 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    42781 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    42841 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    42901 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    42961 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    43021 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    43081 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    43141 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    43201 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    43261 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    43321 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    43381 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    43441 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    43501 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    43561 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    43621 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    43681 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    43741 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    43801 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    43861 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    43921 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    43981 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    44041 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    44101 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    44161 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    44221 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    44281 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    44341 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    44401 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    44461 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    44521 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc
    44581 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    44641 tcgtgtcgtt tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg
    44701 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    44761 cactagtcct gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg
    44821 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    44881 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    44941 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    45001 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    45061 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    45121 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    45181 tagaaaaacg ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    45241 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    45301 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    45361 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    45421 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    45481 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    45541 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    45601 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    45661 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    45721 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    45781 aaaagcaaag caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga
    45841 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    45901 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    45961 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    46021 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    46081 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    46141 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc
    46201 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    46261 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    46321 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    46381 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    46441 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    46501 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    46561 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    46621 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    46681 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    46741 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    46801 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    46861 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    46921 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    46981 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    47041 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    47101 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    47161 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    47221 tggaacagct gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg
    47281 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    47341 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    47401 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    47461 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    47521 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    47581 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    47641 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    47701 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    47761 acgacctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    47821 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    47881 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    47941 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    48001 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    48061 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    48121 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    48181 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    48241 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    48301 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    48361 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    48421 ctttgctttt ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    48481 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    48541 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    48601 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    48661 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    48721 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    48781 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    48841 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    48901 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    48961 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    49021 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    49081 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    49141 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    49201 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
    49261 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    49321 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    49381 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    49441 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    49501 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    49561 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    49621 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    49681 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    49741 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    49801 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    49861 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    49921 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    49981 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    50041 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    50101 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    50161 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    50221 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    50281 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    50341 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    50401 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    50461 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    50521 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    50581 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    50641 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    50701 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    50761 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt
    50821 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    50881 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    50941 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    51001 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    51061 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    51121 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    51181 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    51241 cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    51301 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    51361 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    51421 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    51481 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    51541 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    51601 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    51661 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    51721 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    51781 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    51841 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    51901 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    51961 gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    52021 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    52081 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    52141 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    52201 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    52261 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    52321 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    52381 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    52441 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    52501 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    52561 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    52621 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    52681 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    52741 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    52801 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    52861 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    52921 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga
    52981 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    53041 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    53101 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    53161 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    53221 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    53281 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    53341 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    53401 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    53461 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    53521 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    53581 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    53641 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    53701 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    53761 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    53821 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    53881 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    53941 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    54001 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    54061 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    54121 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    54181 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    54241 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    54301 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    54361 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    54421 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    54481 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    54541 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    54601 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    54661 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt
    54721 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    54781 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    54841 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    54901 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    54961 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    55021 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    55081 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    55141 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    55201 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    55261 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    55321 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    55381 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    55441 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    55501 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    55561 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    55621 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    55681 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    55741 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    55801 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    55861 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    55921 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    55981 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    56041 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    56101 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    56161 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    56221 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    56281 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    56341 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    56401 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    56461 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    56521 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    56581 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    56641 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    56701 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    56761 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    56821 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    56881 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    56941 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    57001 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    57061 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    57121 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    57181 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    57241 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    57301 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    57361 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    57421 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    57481 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    57541 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    57601 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    57661 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    57721 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    57781 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    57841 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    57901 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    57961 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    58021 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    58081 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    58141 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    58201 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    58261 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    58321 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    58381 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    58441 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    58501 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    58561 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    58621 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    58681 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    58741 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    58801 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    58861 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    58921 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    58981 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    59041 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    59101 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    59161 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    59221 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    59281 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    59341 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    59401 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    59461 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    59521 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    59581 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    59641 aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    59701 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    59761 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    59821 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    59881 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    59941 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    60001 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    60061 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
    60121 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
    60181 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
    60241 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
    60301 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc
    60361 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
    60421 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
    60481 tagatttagc gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg
    60541 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
    60601 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
    60661 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
    60721 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
    60781 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
    60841 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
    60901 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
    60961 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
    61021 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
    61081 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
    61141 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
    61201 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
    61261 cccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac
    61321 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
    61381 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
    61441 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
    61501 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
    61561 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
    61621 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
    61681 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
    61741 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
    61801 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
    61861 tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
    61921 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
    61981 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
    62041 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
    62101 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
    62161 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
    62221 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
    62281 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
    62341 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
    62401 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
    62461 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
    62521 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
    62581 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
    62641 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
    62701 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
    62761 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
    62821 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
    62881 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
    62941 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
    63001 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
    63061 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
    63121 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
    63181 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
    63241 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
    63301 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
    63361 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
    63421 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
    63481 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
    63541 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
    63601 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
    63661 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
    63721 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
    63781 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
    63841 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
    63901 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
    63961 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca
    64021 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
    64081 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
    64141 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
    64201 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
    64261 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
    64321 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
    64381 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
    64441 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
    64501 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
    64561 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
    64621 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
    64681 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
    64741 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
    64801 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
    64861 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
    64921 tttcaaacga ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc
    64981 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
    65041 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
    65101 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
    65161 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
    65221 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
    65281 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
    65341 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
    65401 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
    65461 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
    65521 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
    65581 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
    65641 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
    65701 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
    65761 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
    65821 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
    65881 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
    65941 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
    66001 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
    66061 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
    66121 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
    66181 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
    66241 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
    66301 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
    66361 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
    66421 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
    66481 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
    66541 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
    66601 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
    66661 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
    66721 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
    66781 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
    66841 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
    66901 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
    66961 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
    67021 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
    67081 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
    67141 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
    67201 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
    67261 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
    67321 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
    67381 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
    67441 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
    67501 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
    67561 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
    67621 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
    67681 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
    67741 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
    67801 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
    67861 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
    67921 gcaggttgtt ccattaaata aagaatagg
//
LOCUS       DACCQP010000006        63026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCQP010000006 DACCQP010000000
VERSION     DACCQP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.006"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..282)
                     /locus_tag="IVO87_000463"
     CDS             complement(<1..282)
                     /locus_tag="IVO87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448688.1"
                     /translation="MHIKSIVSDEDREELERMLSLGFIIPNQLFKTRQKSRMIIYIFT
                     PVEFLLSINCYLSESYLMFGIGIGTCVACVAWIIWYQMIGKNFEKNVHEF"
     gene            complement(363..2624)
                     /locus_tag="IVO87_000464"
     CDS             complement(363..2624)
                     /locus_tag="IVO87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7448689.1"
                     /translation="MSDQNVKAAQKYLNAMFGGHKDWVKLDEDGKTGTAVMQGIIRAF
                     QIQNGISTITGTVGPLTINTMKKLAIITKMDPNDTPQVNVCLIQCALFCKGYAAGGIT
                     GIYYTSGVNAVKKMQENAGLEVTGKIDWKVWSGLLSLNWFTKVSGGDSNIVLIQQQLN
                     SDWSDVIGVGPCDGIASRQTILSLVGALQAAEGVTTELITDLNSVNFGDATTNAFPGT
                     LQNGQNSTKYVPFNKIAQYGLYFNGYNPGRFDGVFDSTTESKVSEFQEFYGLTGIGLV
                     TKGKVNVSTMKSLLTSKGDTNRAAKACDCATVLNKQQALDIKNAGYTHVGRYLTGSVG
                     KEHTPKYLTSTEVKNIENAGLSVFPIYQDGGYELNYFKDPSQGSVDAQTAILAAERIG
                     IPSGTTIYFAVDFDCYSYQIDTFIIPYFEQIHMIFFSSTNDKNYKVGIYAPRYVCTKV
                     YEAGLASKSFVADMSTGFSCNLGYSMPKNWAFDQFCELNSFSSSPSFPLDKDAYSGRD
                     TGFKKFDAVSTKTDEEIAQENLRAKVKIARNQYVYNVMEPLGYLNKIMDVGVEYDKEI
                     SLGTMMSPQGAIDISTKISTSLESSTGKIYNIKVDIGNDGELTQTCKNQIMEISSNLS
                     DTGIEGADNFGNTIEKIALSVKSGNIAFEINNVFANSVEFSIVFSTSDLLPEEEKEWT
                     ISVALIFTMTLNSNSGLEFNVVEFTKEHSNILAGAVILVLAGALVVNAIPSIIALFSA
                     GAGTVFGLLIQAL"
     gene            complement(2795..4486)
                     /locus_tag="IVO87_000465"
     CDS             complement(2795..4486)
                     /locus_tag="IVO87_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7448690.1"
                     /translation="MKDIKERVRDKNPKIRNPAARLPKELVRSAVLEAKEKPRELHEK
                     SSGQSESPTQYGTEKIESVQYRAASVAGKSIGKTTYQGGKKLAGVTYRKIKERKSRQE
                     EAKAAEEAMEQGAESGKKLIKLKPEQAALAKENGKRQVKAAPRVVKVSGLSQEKIKTQ
                     ASMQKQQMEKSLQAMQKARAAQMARKSAQASAESGKAVLQVTGKGSKLSVQGITAAIQ
                     KGVVALEKMGKWIAAGGSAFLLVFILIVGIIAGAAFSSNSESSESLSEEVLAYTSVIQ
                     QYASQYGIPEYVSAIQAIMMQESGGRGTDPMQCSESPYNTRFPHTPGSITDPDYSIEV
                     GVQTFADCIRQAGCSSPQDLDKLKLAWQGYNYGNGYIGWALQRGGYTEANALQFSQEQ
                     AASHGWSSYGDPQYVPHVMRYYSGGSLFAGLFGNQQIVSVAMGQLGNSGGQKFWSWYG
                     FDSRVEWCACFVSWCADQSGLIASGNVPKFSLCSDGVSWFQGKNKWQSGGTTPTAGMI
                     IFFDWDHDGTSDHVGIVEKCEGGRVYTIEGNSSDQVRQRNYAVDYSSIMGYGVIN"
     gene            complement(4516..4866)
                     /locus_tag="IVO87_000466"
     CDS             complement(4516..4866)
                     /locus_tag="IVO87_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448691.1"
                     /translation="MKKKEEVTITFYAAECGEFHDLGEYTKCRTLEEAYKKYQKYCRT
                     SANMCPAIEFSIHDPESIYSDMEYPLPLSSKDRGDLELVPYYNEHPLVNEAIRQVEQL
                     QKQQEKKKHRDVAR"
     gene            complement(4866..7202)
                     /locus_tag="IVO87_000467"
     CDS             complement(4866..7202)
                     /locus_tag="IVO87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7448692.1"
                     /translation="MPLIKTLSQALRMDKERFKVPKSVQQAIPIQRIWPDGIFQQGTK
                     FSKTYRFTDINYYIASKDNKTEMFLDYSELLNSLDSGISAKITINNRRINKEEFEKSI
                     LLPMKEDGLDHYREEYNEMLLSKITGTNNSIYQERYLTVSVHKRSIDDARTYFARIGT
                     DIVTHLAKLSSTAEGLDAESRLQIFRDFFKGDVPQAFPFDLKQFAKKGTSFKDWMCPD
                     SMEFERDHFKIGDRYGRVLYMQDYASYVKDDMISELCDFSRNLILSIDILPVPTDEAV
                     REIQNRLLGVETNVTNWQRRQNANNNFSAIVPYDMELQRKETKEMLDDLTTRDQRMMF
                     GILTMVHLADSKKQLDSDTELLLSIARKHLCQMATLKWQQVDGLNTVLPYGLRKINAL
                     RTLTTESTAVLIPFHTQEILQPGGIYYGQNAVSKNLLVADRKKLMNGNSFRLGVSGSG
                     KSFSAKEEIVHLALSTDDDILILDPESEFTKLVEALGGQVVKVSATSDNHLNAMDMDA
                     AYGNEKNPLIEKSEFILSVFEQLVGAGNLSAKEKSILDRCAADVYRDYIRSGYTGEVP
                     TLKDMYRQLMLQPEEEARGLALSSELFINGSLNTFAQPTNVNKKNRIMDYDIRELGEQ
                     LMPLGMLVTLDSIFNRVIQNWKEGKTTWIFADEFYLLFRYEYSANFFYRLYKRIRKYN
                     GFVTGLTQNVEELLKSDTARLMLANSEFLILLNQASTDREELAALLNISENQLSYITN
                     VSVGSGLIRCSGNIVPFENTFPKNTDLYKLMTTKPGES"
     gene            complement(7189..7629)
                     /locus_tag="IVO87_000468"
     CDS             complement(7189..7629)
                     /locus_tag="IVO87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7448693.1"
                     /translation="MEVKMNKEIRDYQESMFMGLDLRQCVCSVLAILTAIGIYFGMRE
                     ITGQEITGWLCVLGATPFAACGFFKYHGMTAEQFLWAVIKSELLYPKKLVFQSEDIHY
                     SCMQETISLGEKNQKDGAEIIKEREQRAEAKQRKKRKGRSDAFD"
     gene            complement(7633..8064)
                     /locus_tag="IVO87_000469"
     CDS             complement(7633..8064)
                     /locus_tag="IVO87_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4313 domain-containing protein"
                     /protein_id="HAP7448694.1"
                     /translation="MDIKKTLRYNWEFGRETVAIRVSSYRNNGNLYVGLCHKEGREWE
                     DFGDVTINLPYQFLEPNEAFITGDFTKDMLHFIKEHKLGKVLNETGRSGYATYQKVAF
                     DLARLAEFDPEGVAEHCRFAGIEVPKEKPQKTKKQSRGKER"
     gene            complement(8069..8851)
                     /locus_tag="IVO87_000470"
     CDS             complement(8069..8851)
                     /locus_tag="IVO87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448695.1"
                     /translation="MAELWTLLTENPATFKGGGIWRIMVNINDALTAIAYGLLVLFFA
                     AGIIKTCGSFTDLKKPEHVLKAFIRFALAQGAITYGMELMQALFSIVQGIVTTVMSGS
                     SMAGSVTELPTEIVDKIESVGMLESIPLWIVTLLGSLLITVLSFVMILTVYGRMFKIY
                     MYTAIAPIPISSFAGEPTQSIGKNFIRSYVGVCLEGAIIALACIIFSAYAASPPAVGD
                     TELSAVTIVWNYVGELVFNLLVLVGAIKASDRIVREMMGLGG"
     gene            complement(8999..9316)
                     /locus_tag="IVO87_000471"
     CDS             complement(8999..9316)
                     /locus_tag="IVO87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HAP7448696.1"
                     /translation="MITPMPMGRKQKKWMSLYMALVLTVTVGATTAFAAGDPLSVINN
                     LSTFIFSLIRAIGLILLGFGVVQVGLSLKSHDPSQRANGFLTLAGGVIITFAKEILDL
                     IMA"
     gene            complement(9404..11131)
                     /locus_tag="IVO87_000472"
     CDS             complement(9404..11131)
                     /locus_tag="IVO87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7448697.1"
                     /translation="MLYLVFLPFIWWAAAITACAITPDKNFIQILETLSEKLEQPFFI
                     TYTPYTFKCILIFTAAYFLGIGIYESQKRNYRRGVEHGSAKWGNVSEICRRYCEKQYT
                     NNLLLTQHFRMGLDGYKHKRNLNVLVVGGSGAGKSRTYAIPNIMQCNCSMVITDPKAE
                     LLRKTGGVLERNGYEVRVFDLINPETSWCYNPFAYVRDDKDVLKLINNLIRNTTPKGA
                     QSSDPFWEKSETALLQALMLYLLHEAPPEEQNFPMIMEMLGSAQVKEDDEDYQSPLDI
                     LFERLEMRDPESIAVKQYAIYKQAAGKTAKSILISVGVRLAAFNLKQIANLTCTDELD
                     LYSIGEKKVALFCCIPDADTSMNYLVGMIYSNLFQTLYYVADRKYGGRLPIPVHCIMD
                     EWPNVALPDDFDKILATMRSRGISCSIIIQNIAQMKALFKDSYESLIGNCDEFLYLGG
                     NEKEGHKYVSELLGKETLDTNTYGQTKGRSGSYSVNYQQTGRELLTPDEIRLLDNRKA
                     ILFIRGERPIMDDKYDLKKHVNFRYTEDGGASPYDYAKTPLAHDDLKIDINRLDDYEL
                     LSTEDILGE"
     gene            complement(11152..11406)
                     /locus_tag="IVO87_000473"
     CDS             complement(11152..11406)
                     /locus_tag="IVO87_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HAP7448698.1"
                     /translation="MARLREQYPAGTKICLNSMDGEAGMPQGLEGTVQYVDDAGQLGV
                     KWDNGRSLSLIPGVASFHKKEGQGHPEEKPRKKRESDLQR"
     gene            complement(11447..11758)
                     /locus_tag="IVO87_000474"
     CDS             complement(11447..11758)
                     /locus_tag="IVO87_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448699.1"
                     /translation="MTKYYLKMDNIMKNMFVVALNDELQAQSEQDECLEETKALLKKV
                     SEYDLAQKRLQITAGEQRQIRKALNRLRSKYLAAGRYSDGIDKVIIQVAKPNTSRHMF
                     W"
     gene            complement(11842..12834)
                     /locus_tag="IVO87_000475"
     CDS             complement(11842..12834)
                     /locus_tag="IVO87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448700.1"
                     /translation="MYMDLTDMISGGTEEQQIPEQKLTKEEYAAKKQQEREEVWAEVD
                     SQAQGVFQDGEKLKGFLDFMAECNTQRTPNLLLIYGQHPDFKVVRSYERWREEHRSVK
                     AGEHGITYMISTEYEKDGEMRRGYTIGKGFDISQMSGKPLEERPQRSLTELIGTVLKD
                     QSVRVQIEDDLPDKVQAQYNPRYRTIYIRNGMSEVTTFHALNRELACAALDQHTGTYS
                     RQKVSAQAYCAAYVIAKRYGVDVTGFNLEYIAQRNECGKKDPQELRDFLNDVRQASYS
                     IRNHIERNFGAKEQEYITDEFAIGDPVKIPETKDEKPSEKASGKEKKAKSQPER"
     gene            complement(12834..13640)
                     /locus_tag="IVO87_000476"
     CDS             complement(12834..13640)
                     /locus_tag="IVO87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7448701.1"
                     /translation="MNYGAEPADQVVRYSLEGMEYSLKITGNVAKHVAVFIAAALKDQ
                     KKTRGKTRLTRMLKEQRPMKFFTVPGDRLKEFAKEAKARGILYVVIKNKVNPDQTEVM
                     VFADDAAKMNRVLDRMNLDFVKAESGSMVTEAVEKENAPEKAENQMEKETGENVRTET
                     VVLPEGKIEFELDDAEEMFQIGDEAFPEGNFTQAQEGEVKNPSEPFLRNKNIFSGRET
                     SWEEKNEKPSVRKELGDIRKEQEEKRKRPDRRKNARESRKKRKNMKTKGR"
     gene            complement(13643..14932)
                     /locus_tag="IVO87_000477"
     CDS             complement(13643..14932)
                     /locus_tag="IVO87_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7448702.1"
                     /translation="MAYTSVIPVHRLDRSIEYVKDKEKTTQKADSLEAAIDYAMNRTK
                     TEQAVFEDTIGCTNENAYEDMLATKKRFHKMGGVEGYHLVQSFAEGEVTPELAHLIGQ
                     ELADRLLKGQFEVVITTHLNTRHYHNHIVWNSVSMVDGHKYHSNAKSYYTEIRKISDD
                     LCRKYGLSIIESNQKKSIPYVQWKAEQEGKPTWRTAIRLDIRESVQESYSWSQFLKEM
                     EKRGYTWKLNRKYISLKAPGMERYIRLRSLGKNYSEEEIRGQILQPKVKRVYQKAVQI
                     HPKKKLTGIQALYYSYLYQMGVLPKRPKRSPYAVREDIRKLDRRIEQIEFLMKHDIIT
                     REQLAAYREPLQKQIAELMKERRRLYRNGGSKTGEERLSEINEELKRLRKEVRMTVQI
                     EKHSLEIEARLQEAEEQSQDEKRVEDKERMQKSQEVR"
     gene            complement(14939..15247)
                     /gene="mobC"
                     /locus_tag="IVO87_000478"
     CDS             complement(14939..15247)
                     /gene="mobC"
                     /locus_tag="IVO87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP7448703.1"
                     /translation="MRKRNHVIPVRLNAREVRELNEQVERSGLSREEFMRSLILGAQV
                     HAKPCEHHPELLRKIAGLCNNANQLAHVANASGMASEQSIQEMLRLTKETWHLVKEEW
                     "
     gene            complement(15226..15402)
                     /locus_tag="IVO87_000479"
     CDS             complement(15226..15402)
                     /locus_tag="IVO87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7448704.1"
                     /translation="MLRNGVMIVIALMSGGFIGVIVTCCMVAAKKSDEDLGGMMKEEE
                     KKEGTVMQCENETM"
     gene            complement(15541..15873)
                     /locus_tag="IVO87_000480"
     CDS             complement(15541..15873)
                     /locus_tag="IVO87_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448705.1"
                     /translation="MERMEQALAYVCCSAEESRVKVQKYCRKIYELGYVPICPRFVFS
                     PFLEESDAEDMQGYGRMSHILLRRCRMVVVCGKEVTGTMNTEISMADRLHIICTTLDG
                     LVQIKESK"
     gene            complement(15946..16758)
                     /locus_tag="IVO87_000481"
     CDS             complement(15946..16758)
                     /locus_tag="IVO87_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAP7448706.1"
                     /translation="MMPMTNGLSLEELMKEGTYPEEYTEGENWKILHGDTLKLVKAFQ
                     PGIFDAVITDPPYASGGTKQNERNRTTNQKYSSMSAANALPDFDGDNKDQRSWTHWMA
                     EWLYDVRKACKKGAPICLFIDWRQYPSITDALQWAGWIWRGTAVWDKGNSRPQKGRFR
                     QQAEYIVWGSNGPMPINRPVSCLPGVFRYGNPQNRIHVTEKPLQLMKDVIQICEPRGL
                     ILDPFVGAGTTVLAAVMTGYRAVGIEVTDAYYKLGSDRVKIALEAEQKEDEK"
     gene            complement(16770..17516)
                     /locus_tag="IVO87_000482"
     CDS             complement(16770..17516)
                     /locus_tag="IVO87_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448707.1"
                     /translation="MEEKGTMHVHLLTELTGNLYSEESEWEEEWSESDEYGMPLDGTE
                     LADYEEVIREELERYGEDDLMQYFDGSESIQEKIQSAVVTIENKDGILYGCTKLELNE
                     FLSQEELQEFTEYITGQYSDGWGEGFEQRDIKVDGGTMNVHFWHPNMEQPKMYEKKTE
                     QIPTKPEKQRPKLQLLGHDGNIFSIMGDASTLLKKNKQTAEAKEMRKRVLESGDYYKA
                     LLIISEYVETELSPPPKENKKTKKKNQPER"
     gene            complement(17523..18500)
                     /locus_tag="IVO87_000483"
     CDS             complement(17523..18500)
                     /locus_tag="IVO87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP7448708.1"
                     /translation="MPWVTEEEIQAAKNMTAYEYLRTHQAQRLQKTRTRNEWQLTDHD
                     SFKINELSSKWHWKSRDIGGVSALRFLIEVDGMKFTDAVKLLCEESPSFIPTQSAKKE
                     KPPFKLPERYCNCRRIRAYLNHRGISDEVITYCISHEILYESVPYHNAVFVGKDESGK
                     ARYAFLRGIYEKNGKGFKMEQTGSEKKYSFCIPPERETKRVVVYEACIDALAHMTLEE
                     GKRDKYRLSLGGIAAPKENTQIQSERFKKLPDALEEFLKNHPEIKEIEICTDNDFAGR
                     WAKEQMKKHYEGTYQIICNLPEKEGADYADLAKEKKQTNKCRDRPLERR"
     gene            complement(18500..19663)
                     /locus_tag="IVO87_000484"
     CDS             complement(18500..19663)
                     /locus_tag="IVO87_000484"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7448709.1"
                     /translation="MMRRFYTAESVTEGHPDKVCDQIADAILDECLRYDPSSRVACEV
                     LATRGNVFVAGEITSGYEPPVFDVIRKVLEECGYCTDGIEMDTFVHQQSPDIAKAVSV
                     SKEFRDNIGAKLRDRSRGAGDQGVMVGYACDDTPQLMPMPTVLAHRITRELSACRRSG
                     YIKDIFSDGKAQVTVEYEDGKPVRLESVVVSCQHGAEKSLKKLDAEIREKVLRSALRL
                     LPPDEDTKILINPSGKFVCGGFDADTGLTGRKLMVDTYGSMVPHGGGAFSGKDCSKVD
                     RSAAYMARYIAKNIVAAEFASKCQVSLAYAIGVAEPVMIEVDTFDTGKVCADDCLAAA
                     IPLVFGVTPVQIMESLRLNRPIFRQTAVFGHFGRKEFPWERTDKVLDLQDAIL"
     gene            complement(19749..24125)
                     /locus_tag="IVO87_000485"
     CDS             complement(19749..24125)
                     /locus_tag="IVO87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448710.1"
                     /translation="MKVRKLKTRIAAFGLAVLMGISTLSSANAFAAEQTGSEQQTEVQ
                     ASEQAVTSQKDTSVTADDITKAVSDDTFAVETSMEGIHYDAEKEDVTLVSIKDENGGE
                     YHSDKAGTYIATYMVVPKDKSDSYTITRKVTLTDTEGQAHSEENGGEKQKSDTESEDD
                     SDSPVQNYTDVEIETSEEDASAQAIKELKEDIEEGNVMVLSAAERATSSGSTVTLTKG
                     RTIYYPSYIGNYLTCLFTVNGKIAYCLQSQKASPPSGSYVAQVLDSNKNLQKVLYYGY
                     GGAGDLTGSYLSGKTEDEKYVYTHIAASYAYAGEAGFTGCNYNDLVNAGVIAYINYLF
                     GQEEPPKGELSLSSTKLNAVRDGNIQKTPNITLSGDHRNYVTLSVPENVTAHNLSKGT
                     SVTNGKIQIYGGDTFYLSADLLLTGSYASGNLYGSVGKTWRTLVLTTGDSKQDIGVFE
                     SETAAPVSFSVQWLNMTRIELMKKDVNTQNPLSGAVYGIYTDKKCENLLMTMSATGTD
                     GKAVSDYFDSALKTVYVKEITAPTGYKLNTEVYKVAVTAGKTMTVTATDERVTGKVKI
                     AKIDKETLAFKAQGDSVLRGAVYGLYAKEDIVHPDGTTGVLYKQDSLIAQGVIGDDGT
                     LEFSELYLGEMYVKEITPPEGYTLDTTKYEVSVTYEGQDVVEVTRDLTVKEQVKKQAF
                     QLIKISEDGEQTETDLVAGAGFKVYLISDLTQVKNGKLKPANGESYTASDFKNYDFSK
                     EQVAVTYENGTAVPVPELITDTKGYAVSPELPYGSYVVVESTTPENLKTIDPFVVNVE
                     NDSREPMQWRVFDDRPFEFLLKIVKKDAQTGNTVLKAGASYKIYDVTNKKYVEQVVQY
                     PKKEKISVFETNEEGYLITPQELKCSTYRIEEVKAPEGFVRQGSEESLYDGTTIISPL
                     EQTTKGTYKENPQSGIVITVSSNTAHQIDPDTGAAIVEVEQKNDEQVGSLLLTKKGEQ
                     LTEVTGDSVLAKVKALVSKVRNAVSGKEETGIYKGFKYEETGVEGAEFEIYAKDTIYS
                     PDGAKDEAGNPVVRYEKDDLVAKLTTDENGTAVINNLPLGTYYLKEVVAGENFVLNTE
                     QKEFTLTAEDDTQAVVYEGVTYKNERQKVFVSVEKKDSVTGEKLEGVIFGLYAAEDIL
                     SNQGEVLVEKDTLLEKKATDAKGTLTFDSDLPHGKYYVKEEVRKAGYLPNEEVWNVDA
                     TYENQNLAKIELNKEVENQPTETRITKTDATTGNELEGAKLQVIDKDGNVVEEWVSSK
                     EEHVIYGLPEGSYTLHEELAPYEDGYVSASDVMFEVKEDGSVTKVEMKDEYSKVEISK
                     TDLTTGKELEGAKLQIIRKDGTVLEEWITDGKPHSVEKLPVNEELTLREITAPDGYEI
                     AEDVTFTLKDTMEVQKVEMKDARTPEKTTEKTNAPKTGDNQKIWAFVLLALASAGTAT
                     GVTVYRRKKSKMTDNKKETEEK"
     gene            complement(24199..24681)
                     /locus_tag="IVO87_000486"
     CDS             complement(24199..24681)
                     /locus_tag="IVO87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP7448711.1"
                     /translation="MKDRYYFAYGSNMNLEQMKYRCPAAEVVENVRLENYRLAFRGRA
                     PGNGVATVLPEKGSYVEGVLWKITEACEKSLDFYEGFPSFYGKESIRVKNQDGVEKEV
                     FVYMMNAPHKDVPAKPSKFYLDGILEGCKDNQIPTESVMEAVKRTRQEVKKEKNRYAR
                     "
     gene            complement(24694..25650)
                     /locus_tag="IVO87_000487"
     CDS             complement(24694..25650)
                     /locus_tag="IVO87_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase enzyme"
                     /protein_id="HAP7448712.1"
                     /translation="MIGIKDQYFGTEIEMTGITRQRAAEVVAEMFGTEAYYDGTTYGV
                     WSVIDLEGKKWKFMSDGSIYTQRKVYGRIVDAGGEYSTEMVSPKLSYDEMGKLQEVVR
                     CLRQHGGFVNESCGQHVHVDASNHTPQSLKNALTIMYAKEDILFKALKVQERRANSYC
                     QRVRPEVLEKIRKIPNKSITMDRVRNVWYGGRDGSHTHYDHTRYYALNLHAVFSKGTL
                     EWRCFESTLHAGKVRANITLALAISAQAINQRSTQMKKTLISENPAFTFRTFLLRLGL
                     IGDEYKNVRKHLLANLDGDLAWRYDKSTYECLKKNQRTEGVR"
     gene            complement(25653..26948)
                     /gene="dcm"
                     /locus_tag="IVO87_000488"
     CDS             complement(25653..26948)
                     /gene="dcm"
                     /locus_tag="IVO87_000488"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7448713.1"
                     /translation="MIAATIQFFDLFSGIGGFREGLRRAGNFVCVGHCEVDTYADKNY
                     RLLFDTEGEWYCNDARTIEPERMPDFDLLCAGFPCQAFSIAGKREGFADARGTLFFEI
                     ARILEAKRPSYFILENVPGLLSHDKGRTFCTILSTLSELGYHVEWKVLNSKDFGVPQA
                     RKRVYIVGYFDFRCAGKVLPEPETNGAALVQVRAGSQGKRVYSPKGLSCTLTSQAGGM
                     GGKTGLYDVGVPIKENTKLGYKLAREGDSIDLGYANLNSRRGRVGHQIAHTLTTGVQQ
                     GTLHFVDLSPPPVVTEECRSLNTRQCGVHKYKGECSGVLAEDGARAVLTPAKENVRQN
                     GRRMKEPEEPMFTITATDRHGILYHGRIRRLVPRECLRLQGYYDWQIDKIIDSTSDAQ
                     LYKQAGNGVTVNVIEAIGRLLQKADSELNTQKVSEKGIH"
     gene            complement(27019..27570)
                     /locus_tag="IVO87_000489"
     CDS             complement(27019..27570)
                     /locus_tag="IVO87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448714.1"
                     /translation="MAEMKNICGKIPMDLHEKVREEIELTESSTQKFLQQVIEEHFNR
                     VEGKGEISMGAVRTVAVQVSEELFQRLKVVIGKKHMKQKDFLIQIIEEALEKVEVEWQ
                     SKEQPEESEQTTGELGTEETQPEERTEPERESEPEEEAEPDGESEPKEENKPEGESEP
                     EEETEPEGETDPEESKAEEPTEW"
     gene            complement(27725..28015)
                     /locus_tag="IVO87_000490"
     CDS             complement(27725..28015)
                     /locus_tag="IVO87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation centromere-binding protein
                     ParR"
                     /protein_id="HAP7448715.1"
                     /translation="MTRPVYSFRPNLKNPEHQRAWEKFQSVPEGQKSQYLVQAILEKE
                     DRKWMEEVIQKTIKESVRELGICGTTEQLKTKPSSEEIPDQMMDFLSQMENL"
     gene            complement(28012..28920)
                     /locus_tag="IVO87_000491"
     CDS             complement(28012..28920)
                     /locus_tag="IVO87_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7448716.1"
                     /translation="MIIGIDHGYYAIKTRQVSFPSGIIGYDYEPYTMQNVLQYQGKYY
                     VCGTGRQTLVKNKTSNDNYYLLTLAAIAEEIKHRKAERKTEVILAVGLPLSSFGREKQ
                     GFREYLLRKEQPVRFLYESELYEITIKDVKLFPQGYSALALHPEYLKNEPSVLLVDIG
                     GWTVDLMRLDNAVPNAATCRSLELGVIRCIDETAEQVRRNTGLSVTETQIERVLRRES
                     CSMAEEARRIIQENGRKYIERILSAVTESGFDLRAVPTVFMGGGSAILKRHVTAQDAI
                     CRPVFIEDVHANATGYERIVEQMWTR"
     gene            complement(29108..29440)
                     /locus_tag="IVO87_000492"
     CDS             complement(29108..29440)
                     /locus_tag="IVO87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448717.1"
                     /translation="MEVSIYELLAAARESAKSDYIKGDSILCEKRFHPDTHYMVEIEL
                     LKNDNKLGKKGNYIRKFLTEPEYLPILQKQEKHLIKIKRQAIVQKGNLRYIPPPDRLD
                     RRRERDLL"
     gene            complement(29481..29921)
                     /locus_tag="IVO87_000493"
     CDS             complement(29481..29921)
                     /locus_tag="IVO87_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448718.1"
                     /translation="MFRNKELFLTGRGKSIMDVEMEKRKFLNLCGKRDRALLSDEKRM
                     TLGDMERLTYLTEFFELENYGIALWKEFGDDVKEPFEAMMKMLDEPECIEDRWLDDEA
                     KGEKWLLEFQELALTEEYREWIRNYIDKKYEERGLPYPTGADFD"
     gene            30003..30239
                     /locus_tag="IVO87_000494"
     CDS             30003..30239
                     /locus_tag="IVO87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7448719.1"
                     /translation="MQKIRPDMDIGKNIQAIRYKNNMTQDQVVAKLNLMGISITKSTY
                     AKLETNRMNIKASELVALAKLFHTDINAFFSGLL"
     gene            complement(30567..31124)
                     /locus_tag="IVO87_000495"
                     /pseudo
     CDS             complement(30567..31124)
                     /locus_tag="IVO87_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
     gene            complement(31121..31999)
                     /locus_tag="IVO87_000496"
     CDS             complement(31121..31999)
                     /locus_tag="IVO87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7448720.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(31986..33323)
                     /locus_tag="IVO87_000497"
     CDS             complement(31986..33323)
                     /locus_tag="IVO87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7448721.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(33326..34429)
                     /locus_tag="IVO87_000498"
     CDS             complement(33326..34429)
                     /locus_tag="IVO87_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7448722.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(34503..36011)
                     /locus_tag="IVO87_000499"
     CDS             complement(34503..36011)
                     /locus_tag="IVO87_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7448723.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(35977..36669)
                     /locus_tag="IVO87_000500"
     CDS             complement(35977..36669)
                     /locus_tag="IVO87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7448724.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(36691..37665)
                     /locus_tag="IVO87_000501"
     CDS             complement(36691..37665)
                     /locus_tag="IVO87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448725.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            37965..38837
                     /locus_tag="IVO87_000502"
     CDS             37965..38837
                     /locus_tag="IVO87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7448726.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(39295..41493)
                     /locus_tag="IVO87_000503"
     CDS             complement(39295..41493)
                     /locus_tag="IVO87_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448727.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            41725..42717
                     /locus_tag="IVO87_000504"
     CDS             41725..42717
                     /locus_tag="IVO87_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7448728.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            42777..44198
                     /locus_tag="IVO87_000505"
     CDS             42777..44198
                     /locus_tag="IVO87_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7448729.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            44191..45690
                     /locus_tag="IVO87_000506"
     CDS             44191..45690
                     /locus_tag="IVO87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7448730.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            45729..47123
                     /gene="uxaC"
                     /locus_tag="IVO87_000507"
     CDS             45729..47123
                     /gene="uxaC"
                     /locus_tag="IVO87_000507"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7448731.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            47282..47965
                     /locus_tag="IVO87_000508"
     CDS             47282..47965
                     /locus_tag="IVO87_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7448732.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            47967..48803
                     /locus_tag="IVO87_000509"
     CDS             47967..48803
                     /locus_tag="IVO87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7448733.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            48814..50568
                     /locus_tag="IVO87_000510"
     CDS             48814..50568
                     /locus_tag="IVO87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448734.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(50844..51185)
                     /locus_tag="IVO87_000511"
     CDS             complement(50844..51185)
                     /locus_tag="IVO87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7448735.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(51253..53424)
                     /locus_tag="IVO87_000512"
     CDS             complement(51253..53424)
                     /locus_tag="IVO87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7448736.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            53635..54492
                     /locus_tag="IVO87_000513"
     CDS             53635..54492
                     /locus_tag="IVO87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7448737.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(54563..55420)
                     /locus_tag="IVO87_000514"
     CDS             complement(54563..55420)
                     /locus_tag="IVO87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7448738.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(55405..58107)
                     /locus_tag="IVO87_000515"
     CDS             complement(55405..58107)
                     /locus_tag="IVO87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7448739.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(58120..59409)
                     /locus_tag="IVO87_000516"
     CDS             complement(58120..59409)
                     /locus_tag="IVO87_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7448740.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            59559..60605
                     /locus_tag="IVO87_000517"
     CDS             59559..60605
                     /locus_tag="IVO87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7448741.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            60607..62760
                     /locus_tag="IVO87_000518"
     CDS             60607..62760
                     /locus_tag="IVO87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7448742.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        16789 a        14466 c        11056 g        20715 t
ORIGIN      
        1 aaattcatga acattttttt caaaattttt accaatcatt tgataccaaa taatccaagc
       61 cacacaagcc acacaagttc caatccctat tccaaacatc aaataacttt ccgacagata
      121 acaattaatc gacagtaaaa attctacagg tgtaaaaata tagataatca ttctactttt
      181 ctgtcttgtt ttaaataatt gattaggaat tataaaccca agcgaaagca ttctttctaa
      241 ttcttcccta tcctcatctg acacaattga tttaatatgc attcttctta atcccccttt
      301 tgccagaggt cctctttata aaatcgaacc tctggccatt gtctctttta ttcttttaca
      361 atttacagcg cttgaatcaa caaaccaaaa acagtacctg ctccagctga aaataaagca
      421 ataattgacg gaattgcatt tacaactaat gcacccgcaa gaactaatat tacggctcct
      481 gccagtatat tactatgctc ttttgtgaat tctacaacat taaattcaag accactatta
      541 gaatttaatg tcattgtaaa aatcaaagca accgatattg tccactcttt ttcctcttct
      601 ggcagcaaat ctgatgtact aaatacaatg gaaaactcta cactattggc aaaaacatta
      661 tttatttcaa aagcaatatt accagattta accgataaag caattttttc tatcgtattt
      721 ccaaagttat ctgctccctc tatacctgta tctgataaat ttgatgaaat ctccataatt
      781 tgatttttgc aagtttgtgt caattctcca tcatttccta tatccacttt tatattataa
      841 attttaccag ttgaactttc aagcgatgtg gatattttag tagaaatatc aattgctcct
      901 tgaggactca tcattgttcc cagtgaaatt tccttatcat actccacacc cacatccata
      961 attttattca gataaccaag cggctccatt acattataga catattgatt tcgtgctatt
     1021 tttacctttg cccgaagatt ttcttgcgct atttcctcat ctgtttttgt cgatacggca
     1081 tcgaactttt taaatccagt atctctccct gaatatgcat ctttatccag tgggaacgaa
     1141 ggtgatgaag aaaatgagtt caactcacaa aattgatcaa atgcccagtt cttcggcatc
     1201 gaatatccca aattacagct aaatccagtt gacatatctg ccacaaatga tttacttgcc
     1261 aatcctgctt cataaacttt agtacataca tatcttggtg cataaatgcc gactttataa
     1321 tttttatcat ttgtcgaact gaaaaagatc atgtgaatct gttcaaaata aggtataata
     1381 aatgtatcaa tctgatatga ataacaatca aaatccactg caaagtatat agtcgttcca
     1441 cttggtatac cgattctctc ggctgctaaa attgctgtct gtgcatcaac acttccctgg
     1501 gacggatcct taaaatagtt taattcatat cctccatcct gatatatcgg gaataccgat
     1561 aatcctgcat tttcaatgtt tttcacttct gttgatgtca aatatttcgg tgtatgttct
     1621 tttccaacag aacctgtcag ataacgtcct acatgagtat accctgcatt ttttatatct
     1681 aatgcctgtt gtttatttaa tactgttgca cagtcacacg cttttgctgc cctgtttgta
     1741 tcgcctttac tcgtaagcaa tgatttcata gtagaaacat ttacttttcc ttttgttaca
     1801 agtccaattc ctgtcagacc ataaaactcc tgaaattctg acacctttga ctctgtagtc
     1861 gaatcaaaca cgccatcgaa tcttcccgga ttatatccgt taaaatatag tccgtattga
     1921 gcaatcttat taaatggaac atatttcgtc gagttctgtc cgttctgtaa tgtacccgga
     1981 aacgcatttg ttgttgcatc gccaaaatta actgaattca aatctgtaat taattcagtt
     2041 gtaactcctt cagccgcctg caatgcacca actaaagaca aaattgtctg tcttgaggca
     2101 attccatcac aaggtccaac accaataaca tctgaccagt cactatttaa ttgctgttga
     2161 ataagaacaa tattggaatc cccaccagaa acctttgtaa accagtttag ggataacaat
     2221 ccagaccaaa ctttccaatc aattttgccc gtaacttcca gtcctgcatt ttcctgcatc
     2281 tttttaactg catttactcc tgatgtataa taaattccag taattcctcc agcagcatat
     2341 cctttgcaaa acagagcaca ctgaatcaag catacattaa cctgcggagt atcattcgga
     2401 tccatttttg taataatggc taacttcttc atggtattaa tagtaagtgg tccaaccgtt
     2461 cctgtaatcg ttgagattcc attctgaatc tgaaatgcac gaatgattcc ctgcatcacg
     2521 gctgttccgg tctttccatc ttcatccagt tttacccaat ccttatgtcc accaaacatc
     2581 gcatttaaat atttttgtgc tgcttttaca ttctgatcac tcatattcac tacctcctaa
     2641 ttcaagtcaa ttataaagat ttctaactct ttatatgtct atcactaaaa gttacaataa
     2701 caatttatca atcaactgtg cctattttgt gactattttc caaaggattc attaactccc
     2761 ttataaaatc cttaacaaat tatgaaagaa accatcaatt tatcacccca tatcccatta
     2821 tagaactgta atctactgca taatttctct gccgtacctg atcactggaa tttccctcaa
     2881 tcgtgtaaac tcttccacct tcacactttt ctactattcc tacatgatcc gatgttccat
     2941 catgatccca gtcaaaaaag atgatcattc cagctgtcgg agttgttccg ccactctgcc
     3001 acttattctt tccctgaaac caggacactc catcactgca caatgagaat ttaggaacat
     3061 tcccacttgc aatcagaccg ctctgatccg cacaccagct tacaaaacag gcacaccatt
     3121 ctacccgact gtcaaatccg taccagctcc agaacttctg accgccactg tttccaagct
     3181 gccccattgc aaccgacaca atctgctgat taccaaacaa accagcaaac aggctgccac
     3241 cggaataata ccgcatcaca tggggaacgt attggggatc tccgtaactg ctccatccat
     3301 gcgaagctgc ctgttcctgt gaaaactgca atgcattggc ttctgtataa ccgcctcttt
     3361 gcaatgccca tcctatgtag ccatttccat agttatatcc ctgccatgca agtttcagct
     3421 tgtccagatc ctgtggactg ctgcatcctg cctgtctgat acagtctgca aaggtctgta
     3481 ctccaacctc tatcgaataa tccggatcag taatagaacc aggagtgtgt gggaatcttg
     3541 tattatatgg actttccgaa cactgcatcg ggtctgttcc cctgcctcca gattcctgca
     3601 tcatgatggc ctgtatcgca gacacatatt ccggaatgcc gtattgggat gcatactgct
     3661 gtatcacaga agtatacgca agcacttctt cacttaggga ttcggagctt tccgaattac
     3721 tgctgaaagc tgctcctgca atgattccca caatcagaat aaataccaga agaaatgcac
     3781 tacctccggc agcaatccat tttcccattt tttcaagagc tactacccct ttttgaatcg
     3841 ctgctgtaat tccctgtacg gataactttg aaccctttcc agttacctga aggactgctt
     3901 ttccactttc tgccgatgcc tgtgctgact ttcttgccat ctgggctgct cttgctttct
     3961 gcatcgcctg aagacttttt tccatctgct gtttctgcat ggatgcctgt gtctttattt
     4021 tttcttgaga cagaccggat acttttacca ctctcggtgc tgctttcacc tgccgtttac
     4081 cattctcctt ggcaagtgct gcctgctccg gtttcaactt gattaatttc tttccgcttt
     4141 ctgccccctg ctccattgct tcttcggcag ctttcgcttc ttcctgtctg ctttttcttt
     4201 cctttatctt cctgtaggtc actccagcaa gtttttttcc accttgatat gtggttttcc
     4261 ctatactttt tcctgccaca ctcgccgctc tgtactgtac ggattctatt ttttcagttc
     4321 catactgtgt tggggattct gactgcccac tgcttttttc atgtagttcc cttggctttt
     4381 cttttgcttc cagaaccgct gagcgaacca gttctttcgg aagtcgggca gccggatttc
     4441 gaatcttcgg attcttatcc cttacccgtt ccttgatatc tttcatccgg tcaccctctt
     4501 tccgtatctc ttatctcatc gggcaacatc ccgatgtttc tttttttcct gctgtttctg
     4561 caactgctcc acctgtctga ttgcctcatt taccagtgga tgttcgttgt aatatggcac
     4621 aagttccaag tctcccctgt cttttgatga caacggtaat ggatattcca tgtctgaata
     4681 aattgattct ggatcatgaa tagaaaactc aatagccgga cacatattgg cagatgttct
     4741 acaatatttc tggtactttt tatatgcctc ttccagcgtt ctgcattttg tatattctcc
     4801 taaatcatgg aactcaccgc actctgcagc ataaaaagtt atcgttactt cttctttttt
     4861 cttcattatg attctcccgg ttttgttgtc attagtttat aaaggtctgt attctttggg
     4921 aacgtattct caaatggtac gatattccca gagcaacgga ttaggccact gccaaccgat
     4981 acatttgtaa tataggacag ttggttttcg gaaatattca aaagagctgc caattcttcc
     5041 ctgtctgtac ttgcctgatt caaaagaatc aaaaactcac tgtttgccaa catcagtctt
     5101 gcagtgtccg atttcagaag ttcctcaaca ttttgggtta aacctgtcac gaatccattg
     5161 tattttctga ttctcttgta cagtcggtag aagaaatttg cactgtattc atagcggaat
     5221 aacagataga actcatcggc aaatatccat gtcgtttttc cttctttcca gttctgaatt
     5281 accctgttga aaatactgtc taaggtaacc agcattccca gtggcataag ctgttccccc
     5341 agttcccgga tatcataatc catgatcctg ttcttcttat tcacattggt cggctgtgca
     5401 aaggtgttca ggcttccatt gataaaaagt tcggatgaca gtgcaagtcc ccttgcctct
     5461 tcttccggct gtaacatcaa ctgtcggtac atatctttca atgtcggaac ttctccggta
     5521 taaccacttc ggatataatc ccggtacaca tctgcagcac agcggtccag gattgacttt
     5581 tcttttgctg acaggtttcc tgctccaacc aactgttcaa atacagacag aataaactcc
     5641 gatttttcaa tcagtggatt cttttcattg ccgtaggctg cgtccatatc cattgcattc
     5701 aggtgattgt ctgatgtcgc agatactttt acaacctgtc cacccagagc ctctactaat
     5761 tttgtgaatt cggactccgg atcaagaatc aggatatcgt catcggtact caatgccaga
     5821 tgcacaatct cttcttttgc cgaaaagctc tttcctgatc cactgacgcc caaccggaaa
     5881 ctgtttccat tcatcagttt cttccggtcg gctaccagaa ggttcttgct gactgcattc
     5941 tgcccataat agataccgcc cggctgcagg atttcctgtg tatggaacgg gattaaaaca
     6001 gccgttgatt ccgttgtcaa tgtacgcagg gcattgatct ttcgcagtcc gtaaggaagc
     6061 actgtattga gtccgtccac ttgctgccac tttaatgttg ccatctggca cagatgcttt
     6121 cttgctatcg acaataataa ttccgtatcg gaatccaact gttttttact gtctgccaga
     6181 tgtaccattg tcaaaattcc gaacatcata cgctggtctc tggttgtcag atcatcgagc
     6241 atttccttgg tttcttttct ctgaagttcc atatcgtatg gtacgattgc cgagaagtta
     6301 ttattagcgt tctgccttct ctgccagttg gtcacgttgg tttccacacc aagcagtcgg
     6361 ttctggattt cccttaccgc ttcatccgta gggactggca ggatatcaat cgacaatatc
     6421 agattccggc tgaagtcaca cagttctgaa atcatgtcat ctttaacata acttgcataa
     6481 tcctgcatat acaggactct tccgtacctg tcaccgattt taaaatggtc acgttcaaat
     6541 tccatagaat ccggacacat ccagtccttg aaacttgttc ccttcttggc aaattgtttc
     6601 agatcaaacg gaaatgcctg tgggacatct cccttgaaga agtccctgaa aatctggagc
     6661 cggctctctg cgtccagtcc ttctgctgtg gacgataact ttgccagatg tgttacgatg
     6721 tctgttccga tacgtgcaaa ataggttctt gcatcgtcaa tcgatctttt atgtacactg
     6781 acagtcagat accgttcctg atagatgctg ttgtttgttc ctgtaatctt agacaggagc
     6841 atttcgttgt attcttcccg atagtggtcc agtccatctt ccttcattgg aagcaggatt
     6901 gatttttcaa attcttcttt attgattctc cggttattga tggtaatttt ggcagaaatg
     6961 ccggaatcca gtgaatttaa gagttcggaa taatccagaa acatttctgt cttattgtcc
     7021 ttactcgcaa tataatagtt gatatctgta aaacgatatg tttttgagaa tttcgttccc
     7081 tgttgaaaaa ttccatccgg ccagattcgc tggatgggaa ttgcctgttg gactgatttc
     7141 ggaactttga agcgttcctt atccatccga agtgcctgac ttaaggtttt aatcaaaggc
     7201 atcactcctt cccttgcgtt tctttctctg ttttgcttct gctctttgct cccgctcttt
     7261 tatgatttcc gctccatcct tttgattctt ttctcccagc gagatggtct cctgcatgca
     7321 ggagtagtgg atatcttccg actgaaacac cagtttcttc ggatacagca attcggattt
     7381 gatcactgcc cataaaaact gttcggctgt cataccatga tatttgaaaa agccacaggc
     7441 agcaaatgga gtagcaccaa gcacacacag ccatccggtt atttcctgtc cggtaatttc
     7501 acgcattcca aagtaaattc cgattgctgt aagaatcgca agaacagaac acacacattg
     7561 tctcaggtcc agtcccataa acatggattc ctgatagtcc cggatttctt tattcatctt
     7621 gacttccata acttatcgct cctttcctct gctttgtttc ttcgttttct gtggtttttc
     7681 tttcggtact tctatcccgg caaatctaca gtgttctgca acgccttccg gatcaaattc
     7741 cgcaagtctt gctagatcaa acgctacctt ctgataggtt gcatatcctg atcttcctgt
     7801 ttcattcaac acttttccta gtttatgttc cttgataaaa tgaagcatat ccttggtaaa
     7861 atctcctgtt ataaaagcct catttggctc taagaactga tagggcagat taatggttac
     7921 atctccaaaa tcttcccatt ctctcccttc cttatggcag agtcctacat ataaatttcc
     7981 attgttccga taagagctca ctcgtattgc tactgtctca cgtccaaatt cccagttata
     8041 tcttaatgtt ttctttatat ccatattttt aacctccaag tcccatcatt tctctgacaa
     8101 tacggtcaga tgccttaatt gctccaacca gcaccagtaa attaaatacc agttctccga
     8161 catagttcca cacgattgta actgcactca actccgtatc tccgacagcc ggagggcttg
     8221 ctgcataagc agaaaagata atacatgcca gtgcaataat tgccccttcc agacatacgc
     8281 ctacataaga acggataaag ttctttccaa tggactgcgt gggttctcct gcaaatgagg
     8341 aaatcggaat cggggcgatt gccgtataca tatagatttt gaacattctg ccatacactg
     8401 tcaggatcat tacaaatgac aatacagtga tcagcagact gccaagcagt gtaacaatcc
     8461 acaacggaat ggattccagc attcccacgc tctcaatctt atctacaatc tccgttggca
     8521 gttccgtcac gcttcctgcc atactggagc cactcattac ggttgtcacg attccctgca
     8581 caatggaaaa cagtgcctgc ataagttcca tcccgtatgt aattgctccc tgagcaagtg
     8641 caaaccggat aaatgctttt agcacatgtt ccggcttttt cagatctgta aaactgccac
     8701 acgttttaat gattccggct gcgaaaaata ataccagtaa tccataagca atggctgtca
     8761 gtgcatcatt aatatttacc ataatccgcc agatgccgcc gcccttaaaa gttgccggat
     8821 tttcggtcag cagagtccac agttctgcca tcttttcact ccatgtggat agtgctgagt
     8881 ttagattctg tacaatccag ttatcactca aaaactctca cctccttaaa catatttttg
     8941 aatacaggag taccttccgg cactcctgct gtttcctgat atattttttc aaagaggctt
     9001 acgccataat caaatcgagg atttctttgg cgaacgtaat aattacccct ccggccagtg
     9061 tcagaaatcc atttgccctc tggcttggat catggctttt taaagacaga ccaacctgca
     9121 caacaccaaa tccaagcaaa atcaaaccga tcgcacgaat cagagaaaaa ataaaggtag
     9181 acaggttatt gataacggac aacggatctc cggctgcgaa tgcggtcgtt gctccaaccg
     9241 ttacggtcag taccagtgcc atataaaggc tcatccactt tttctgtttt ctccccatcg
     9301 gcatcggggt tatcattttt tcagttgtat tcttttttgt tctatttttt tctcctgtga
     9361 gaagtttctt caatacacat cactccaatc catcaaaaaa ctgctattca ccaaggatgt
     9421 cctcggtaga cagcagttcg taatcgtcta atctgttaat gtcaatcttt aggtcgtcgt
     9481 gagccagtgg tgttttcgca taatcataag gcgatgcacc accgtcctct gtgtatctga
     9541 aattcacatg cttttttaaa tcatatttat cgtccatgat cggacgctcc ccacgaataa
     9601 acaaaatcgc tttgcggtta tctaaaagcc gtatttcatc cggtgttaaa agttcccttc
     9661 ctgtctgctg ataattcacc gaatagctgc cgctacgccc ttttgtctgt ccgtaggtat
     9721 tggtatccaa tgtctctttt cccaataatt ccgagacata cttatgtcct tctttttcat
     9781 ttccacccag atataaaaat tcatcgcagt tgccgatcag actctcatac gagtctttaa
     9841 ataaggcttt catctgtgca atgttctgga tgatgatact gcaggaaatc cctctggaac
     9901 gcatggttgc cagaatcttg tcaaaatcat ccggcaaggc tacgtttggc cattcatcca
     9961 taatgcagtg taccggaatc ggaagccttc ctccgtactt ccgatctgcc acataataca
    10021 gtgtctgaaa cagattactg taaatcatgc cgaccagata attcatgctg gtgtctgcat
    10081 ccggaataca gcaaaacagt gctacttttt tctccccaat actgtataag tccagttcgt
    10141 ccgtacaggt caggttggcg atttgtttca gattgaacgc tgccagacgg acaccaaccg
    10201 atatcaaaat agactttgcg gtctttccgg ccgcctgctt ataaatggca tactgcttga
    10261 ccgcaatact ctctgggtcc cgcatttcca gtctttcaaa caaaatgtcc aatggtgact
    10321 ggtaatcctc atcatcttct ttgacctgtg cgcttccaag catttccata atcatcggga
    10381 agttctgttc ttccggtgga gcttcatgca gtaaatacag cataagtgcc tgtaaaagtg
    10441 cagtctctga cttttcccag aacgggtccg agctttgtgc acctttcggt gtcgtgttgc
    10501 ggatcaggtt gttaattagt ttcagcacat ccttgtcatc ccggacatac gcaaacggat
    10561 tataacacca ggaagtctcc ggattgatca aatcaaaaac ccggacttca tatccattcc
    10621 gttccagaac acctcctgtt ttgcgcagaa gttccgcttt cggatctgtg atcaccatcg
    10681 agcagttgca ctgcatgatg ttcggaatcg cataggtccg gctctttcct gccccggaac
    10741 ctccgactac cagcacattt aaatttcgtt tatgcttata tccatccagt cccatgcgga
    10801 aatgctgtgt cagaagcaga ttattcgtgt actgcttttc gcagtaacgt ctgcagattt
    10861 cggacacgtt gccccatttt gcggaaccat gttccacgcc tcttctataa tttctttttt
    10921 gggattcata aataccaatg cctaagaaat aggctgctgt gaatatcaga atacatttga
    10981 acgtatacgg tgtatacgtg ataaaaaacg gctgttcgag tttttcgctc agcgtttcta
    11041 agatttgaat aaaattctta tccggtgtaa tggcacaagc ggtaatcgct gccgcccacc
    11101 agataaatgg cagaaagaca agatacagaa tccagtcctg cttttttgta cttatctctg
    11161 caagtcagat tctcttttct tgcgtggctt ttcttctgga tgtccctgtc cttctttctt
    11221 atgaaaggaa gccactcccg gtatcaatga gagacttctg ccgttgtccc acttaacacc
    11281 aagctggcct gcatcatcca catactgcac agttccttcc agtccctgcg gcatacccgc
    11341 ttctccatcc atcgaattca gacagatttt tgttcctgcc ggatactgtt ctctaagtct
    11401 tgccactttt tctcttgaat aaatgaccaa tcaaccactc ctttctctac cagaacatgt
    11461 gtctgctagt attcggtttt gcaacctgga taataacttt atctattcca tcgctgtatc
    11521 ttccggctgc aagatattta cttctgagtc tgttcagtgc cttacggatc tgcctttgtt
    11581 ccccggctgt aatctgcagt cgtttctgag cgaggtcgta ttcacttact tttttcagaa
    11641 gagctttcgt ttcttccaaa cattcatcct gctcactctg tgcctgtagc tcatcattca
    11701 gtgccaccac gaacatattt ttcataatgt tatccatctt caaatagtat tttgtcatct
    11761 ggctgacctc ctttttttgt tacaccaacc atatcacaag acctccgtaa tagctactcc
    11821 aaccgtccaa ctttttcact tttatcgttc cggctggctc tttgcttttt tctcttttcc
    11881 ggatgctttt tcagatggtt tttcatcctt ggtttccggt atttttacag ggtctccgat
    11941 tgcgaactca tcggttatat actcctgttc ctttgcacca aaattgcgtt cgatatggtt
    12001 tcgtatactg taggaagcct gacggacatc atttaaaaag tctctcagtt cctgcggatc
    12061 tttctttccg cattcattcc tctgtgcaat atactccaga ttgaatccgg taacatccac
    12121 accataacgt ttcgcaatca cataggctgc acaataagcc tgtgcactga ctttctgtcg
    12181 gctgtaggtt ccagtatgct ggtccagtgc tgcacacgcc agttctctgt tcagtgcatg
    12241 gaatgtcgtt acttcactca ttccgttgcg gatataaatg gtacggtatc tcggattgta
    12301 ctgggcctgc actttgtctg gcagatcatc ctcaatctgc acacgtaccg actggtcttt
    12361 taagacagtt cctatcagtt ctgtcagact tcgctgaggg cgttcttcca atggctttcc
    12421 tgacatctga ctgatgtcaa atccttttcc gatcgtatac cctctccgca tttcaccgtc
    12481 tttttcgtat tcagtggaaa tcatataagt aatgccatgc tcccctgctt ttactgaacg
    12541 gtgttcttct ctccagcgtt cataagacct tactacctta aaatctggat gctgtccgta
    12601 aatcaaaagc agattcggag ttctctgtgt attacactct gccataaaat ccagaaagcc
    12661 tttcagtttt tctccatcct gaaaaactcc ctgtgcctgt gaatctactt cagcccatac
    12721 ctcttctctt tcctgctgct tcttggctgc atattcttcc ttggtcaatt tctgttcagg
    12781 aatctgctgt tcttccgttc cacctgaaat catatctgtt aaatccatat acattacctg
    12841 cctttcgttt tcatattctt acgtttcttc cggctctctc tggcattttt ccgtctgtct
    12901 ggacgcttgc gtttctcttc ctgttcctta cggatatctc cgagttcttt ccgcacagat
    12961 ggtttttcat tcttttcttc ccagctagtt tcccgaccgg aaaaaatatt tttgttgcgc
    13021 aagaaaggtt cggatggatt ctttacttcc ccctcctgcg cctgtgtaaa atttccttcc
    13081 ggaaaagcct catccccgat ctgaaacatt tcttccgcat catccagttc aaattctatc
    13141 ttgccttccg gcaggacaac ggtctctgtc cgtacattct ccccggtttc tttttccatc
    13201 tggttctctg ccttctccgg tgcattctct ttttccactg cttctgtaac catagagcct
    13261 gattctgctt ttacaaaatc cagattcatc cggtctaata cccggttcat ctttgctgca
    13321 tcatctgcaa ataccatgac ctctgtctga tccggattca ccttattctt gataactaca
    13381 tataagatgc cccttgcctt ggcctctttt gcaaattctt tcagacggtc acccggaaca
    13441 gtaaaaaatt tcatcgggcg ctgttccttt aacattctgg taagtcttgt tttccctctt
    13501 gtctttttct ggtccttcaa agcggcagca ataaagactg ccacatgctt tgctacattc
    13561 ccggtgatct tcagcgaata ctccatccct tccagactgt accgtaccac ctgatctgcc
    13621 ggttctgctc cgtaattcat acttatctca cctcctgact tttctgcatt ctttctttat
    13681 cttccacacg tttttcatcc tgactctgtt cttctgcttc ctgaagtctc gcttcgattt
    13741 ccaaggaatg tttctcaatc tgaacggtca tacgaacttc cttacggagt cgcttcaatt
    13801 cctcattgat ttcggacaat cgttcttccc ccgtcttgct gcccccattc cgatacaatc
    13861 ttctgcgttc tttcataagt tccgcaatct gcttttgcaa tggctctcgg tatgcggcaa
    13921 gctgctctct ggttatgatg tcatgtttca tcagaaattc aatctgttca atcctgcggt
    13981 ccagtttccg gatatcctct ctgacagcgt atggactcct tttcggtctt tttggcagta
    14041 cccccatttg atataaatag gaataataca gtgcctgaat cccggtcagt ttctttttgg
    14101 gatgaatctg tactgccttt tgatagactc gtttcacctt gggctgtagt atctgcccac
    14161 gtatttcctc ttcagaataa ttttttccaa gggaacgcag acgaatatac cgctccattc
    14221 ccggagcttt taaagaaatg tatttcctgt tcagtttcca tgtataaccc cgcttttcca
    14281 tctcttttag gaattgtgac caggagtagc tttcctgtac cgattccctg atatccaaac
    14341 ggatcgccgt tctccatgtc ggctttcctt cctgttctgc tttccactgt acataaggga
    14401 ttgatttttt ctgattggat tcaatgatag acagaccata ttttctgcac aggtcatcgg
    14461 aaatcttacg gatctccgta taataactct tggcattact atggtactta tgtccgtcca
    14521 ccatgcttac agaattccag acaatatgat tgtgatagtg tctggtatta agatgtgttg
    14581 ttatgacgac ttcaaattgt ccttttaaga gccggtctgc caattcctgc ccgatgagat
    14641 gtgccagttc cggtgtcact tcaccttctg caaaactctg taccagatgg tatccttcta
    14701 caccgcccat cttatggaat ctctttttcg ttgccagcat atcttcatac gcattttcat
    14761 tggtacatcc gatggtatct tcgaagaccg cctgttccgt cttggtccga ttcatggcat
    14821 aatcgatggc agcctccaga gagtccgctt tctgtgtggt tttttcttta tctttcacat
    14881 attcgataga acggtccagg cggtggacgg ggataacact tgtataagcc atccggcatt
    14941 accactcttc ctttaccaga tgccatgttt cttttgtaag ccgcagcatt tcctgaatac
    15001 tctgctcgct tgccatacca gaagcattgg caacatgtgc aagctgattg gcattgttgc
    15061 ataagcctgc aatctttcgg agcaattcgg gatgatgctc acagggtttt gcatgaacct
    15121 gcgctcctaa aatcaacgaa cgcataaact cttcccggct cagaccgctt ctttcgacct
    15181 gctcattcag ttccctgact tcccttgcat taagccgtac cgggatcaca tggtttcgtt
    15241 ttcgcattgc atcaccgtcc cttctttttt ctcttcctct ttcatcatac cgccaagatc
    15301 ctcatcggat tttttcgctg caaccatgca acaggtcact atcactccta taaatcctcc
    15361 gctcatcaat gcgattacaa tcattactcc atttcttaac atgacttgat ccttcctttc
    15421 catttccgtc tgtgtccggg ggattggggt tctccccaaa gtgcgtttgg atatccaaac
    15481 gctatgcttg caaaacgagt accgccagtt tctgtagcac cggattgact cgattctgat
    15541 ttattttgat tcttttatct gtaccagtcc atcaagtgtc gtacaaatga tatggagtct
    15601 gtctgccata ctgatttcgg tattcattgt tcctgtcact tctttcccac atacgactac
    15661 catccggcat ctccgtaaaa ggatatggga cattcttcca tatccctgca tatcttctgc
    15721 atcagactct tccaagaatg gtgagaatac aaatctcgga cagattggta catacccaag
    15781 ttcatagatt tttctgcaat atttctgcac ttttacccgg ctttcttctg ctgaacagca
    15841 cacataagcc agcgcctgtt ccatacgctc catctccttc ctgcatccag ttggatgcca
    15901 ctcattttct ctataaaaaa caagatgtcc aattggacat cccggctact tttcatcctc
    15961 tttctgctct gcttctaatg caattttcac tctgtcactt cccagtttgt agtaggcatc
    16021 tgttacctca attcccactg cacgataacc tgtcataacc gctgcaagga cagttgttcc
    16081 tgctccgaca aacgggtcca ggatcagccc tctcggttca caaatctgga taacatcttt
    16141 cataagctgc aacggtttct ctgtcacatg aatccggttc tgcggattgc catatcggaa
    16201 gacgcccggc agacaagata ccggacggtt gatcggcatc ggtccgttac tgccccacac
    16261 aatatattca gcctgctggc ggaaacggcc tttctgtgga cgggagtttc ccttatccca
    16321 caccgctgtt cctcgccaga tccatcctgc ccactgcaat gcatcggtaa tcgatggata
    16381 ctgacgccag tcaatgaaca gacagatcgg agcacctttc ttgcaggctt tccgcacatc
    16441 atagagccat tcagccatcc agtgcgtcca agatctctgg tctttgttat ccccatcgaa
    16501 atccggaagt gcattggcag cactcatact gctgtatttc tgatttgtag tacgatttct
    16561 ttcattctgt tttgttccac cggatgcata cggcggatcg gtaataactg catcaaagat
    16621 tcccggctgg aatgctttga ccagttttaa tgtatctcca tgtaatattt tccagttttc
    16681 cccttcagta tattcctccg ggtatgttcc ctctttcata agttcttcca aactcaggcc
    16741 atttgtcata ggcatcatcc tttcttttct cagcgttccg gctgattttt cttctttgtc
    16801 tttttatttt ctttgggcgg cggactcaac tcagtttcta catattcgct gataatcaaa
    16861 agtgctttgt aatagtcccc actttccaat acccttttac gcatttcttt tgcttctgct
    16921 gtctgtttat tctttttcaa aagagttgag gcatcgccca taatggaaaa aatattccca
    16981 tcatgtccaa ggagctgtaa cttgggacgt tgtttctcag gctttgtcgg aatctgctct
    17041 gttttctttt catacatttt cggctgttcc atattaggat gccaaaaatg cacattcatc
    17101 gttccaccat ctaccttgat atccctctgt tcaaatcctt caccccaccc gtcactgtac
    17161 tgccctgtaa tgtattcggt gaattcctgt agttcttcct gacttaaaaa ttcatttaat
    17221 tccagtttgg tacagccgta caggattccg tctttgtttt ctatcgtaac aactgcactt
    17281 tgtatctttt cctgaatgga ctcacttccg tcaaaatact gcatgagatc atcttcccca
    17341 tagcgttcca gttcttctcg gatgacttct tcataatcgg caagttctgt tccatctaat
    17401 ggcattccat attcatcact ctccgaccac tcttcttccc attcggattc ttctgaataa
    17461 agatttccag tcagctccgt caacagatgt acgtgcatcg ttcctttttc ttccattctc
    17521 tatcaccttc tttctaatgg tctgtccctg catttatttg tctgcttttt ttctttcgca
    17581 aggtctgcat aatctgcccc ttctttttcc ggcagattgc aaataatctg atacgttccc
    17641 tcgtaatgtt ttttcatctg ttctttcgcc catctcccgg caaagtcatt atcggtgcag
    17701 atttcaattt ctttgatttc cggatggttc ttcagaaact cttccagtgc atccggcagc
    17761 tttttaaaac gttcactctg tatctgtgtg ttttctttcg gagcagcgat tcctccaagg
    17821 gaaagacgat atttatccct ctttccttct tccagtgtca tgtgggccag tgcatcaata
    17881 cacgcttcat atacgacaac tcgttttgtt tctctttcag gtggaataca aaagctgtac
    17941 tttttttcac tcccggtctg ctccatcttg aatcctttcc cgtttttttc atagattcct
    18001 cgcaggaatg catatcttgc ttttccggat tcatcttttc caacaaaaac agcgttatgg
    18061 tacgggacac tttcataaag gatttcatgc gaaatgcaat acgtgatcac ttcatcgctg
    18121 attcctctgt ggtttaaata agccctgatt cgtctgcaat tacaatatct ctccggcagc
    18181 ttaaagggtg gtttttcctt tttcgcagac tgtgtcggaa taaaagacgg actctcttcg
    18241 cacaacagtt ttacggcatc tgtaaatttc attccatcta cttcaatcag gaaccgcagt
    18301 gcggacacgc caccgatatc tctggatttc caatgccatt tgctggacag ttcattgatt
    18361 ttaaaactgt catgatccgt caactgccat tcatttctgg ttcttgtctt ttgcaaccgc
    18421 tgtgcctgat gcgtccgcaa atactcatac gcagtcatat tttttgctgc ctgaatctct
    18481 tcttccgtta cccagggcat tacagaattg catcctgaag atccagtacc ttatccgtcc
    18541 gctcccaagg aaactccttt cttccaaaat gaccaaaaac tgccgtctgc cggaaaatcg
    18601 gacggttcag tcgaagggat tccatgatct gaacaggtgt cactccaaac accagtggga
    18661 ttgctgccgc cagacaatca tccgcacaca ctttccctgt atcaaaggta tccacctcaa
    18721 tcataaccgg ctctgctact ccgattgcat aagccagaga cacctgacac ttgcttgcaa
    18781 actcagcagc tactatattt tttgcgatat accttgccat ataagctgcc gaacggtcta
    18841 ctttcgaaca gtcctttcct gaaaatgcac cgccgccatg cggaaccata cttccataag
    18901 tatccaccat cagctttctt ccggtcagtc cggtatctgc atcaaaccct ccacacacaa
    18961 attttcctga cggattgatc agaatcttcg tatcctcatc cggcggcagt aatctgagtg
    19021 ctgagcgaag cactttctct ctgatttcgg catctaactt cttcaggctc ttttctgcac
    19081 catgctggca ggaaacaaca acactttcca gtctcactgg ctttccatct tcatattcta
    19141 cggttacctg tgcttttcca tccgaaaaga tatctttgat atatccgctt cttctgcaag
    19201 ccgaaagttc acgtgtgatc cggtgtgcca gaacagtcgg cattggcata agctgcggag
    19261 tatcatcaca cgcatatccg accataacac cctgatctcc agctcccctg cttctatctc
    19321 ttaattttgc gccgatatta tcccgaaatt ctttcgacac actcactgct ttcgcaatat
    19381 ctggactctg ctggtgtaca aacgtatcca tttctatccc atctgtacaa tatccacact
    19441 cttctaaaac ttttcggatc acatcaaaca ctggtggttc atatccgctg gtgatttctc
    19501 ctgctacaaa cacatttcct cttgtagcaa ggacttcaca cgccactctc gaagatggat
    19561 cgtaacgcag acattcatct aagattgcat ctgctatctg atcgcatact ttgtctgggt
    19621 gtccttctgt tactgattct gctgtataaa aacgtctcat cattcttctc cttatataca
    19681 gacagggcag aagctgcttt atgcacatct cctgccctgt cagattggtt tatcctgtaa
    19741 ttcttatttt atttttcttc tgtttctttt ttgttgtctg tcatttttga cttctttctg
    19801 cggtatacag tcactcctgt agctgttcca gcagatgcca gcgcaagcag aacaaacgcc
    19861 catattttct ggttatcacc tgtttttggt gcatttgttt tttctgttgt tttctccggt
    19921 gttctggcat ctttcatttc tactttctgg acttccattg tatctttcaa tgtaaatgtg
    19981 acatcttccg caatctcgta accatccggt gctgtgatct cacgcagagt aagttcttca
    20041 ttgactggga gtttttcaac ggaatgtggt tttccatctg taatccactc ttccagaaca
    20101 gttccgtctt tgcggataat ctgaagcttt gccccttcca gttcttttcc ggttgtaagg
    20161 tctgtttttg aaatttccac tttggaatat tcatctttca tctcaacctt tgtcacactg
    20221 ccatcttctt tgacttcaaa catcacatcg ctggcagaca catagccatc ttcataagga
    20281 gccagttcct catgtaaggt ataacttcct tccggcagtc cgtaaatgac atgttcttcc
    20341 ttactgctta cccattcttc cacaacattt ccgtctttat caatgacctg cagtttggct
    20401 ccttccagct catttccggt agttgcatct gtttttgtaa tgcgtgtttc tgtcggctga
    20461 ttctcaactt ctttgttcag ttcaatcttt gcaagattct gattttcgta agttgcatct
    20521 acattccata cttcctcatt tggaagatat ccagcttttc tgacttcctc tttgacatag
    20581 tattttccat gtggaagatc actgtcgaaa gtaagtgttc cctttgcatc cgttgctttc
    20641 ttttccagca aggtatcttt ctctacgaga acctctccct gattagaaag aatatcttct
    20701 gcagcataca gtccaaaaat aacaccttcc agcttttcgc ctgtgacaga atctttcttt
    20761 tctactgata cgaaaacttt ctgtctttca tttttatagg taacgccttc ataaacaact
    20821 gcctgtgtat catcctctgc tgtcagggta aattctttct gttctgtatt taggacaaaa
    20881 ttctctcctg ctacaacttc tttcagataa tacgttccca gtggcagatt gttgataact
    20941 gctgttccat tttcatctgt agtcagtttg gctaccaggt catctttctc atatcggaca
    21001 actggatttc ctgcttcatc ctttgctccg tccggtgaat agatggtatc tttggcatat
    21061 atttcgaatt cagcgccttc tactccggtt tcttcatatt taaagccttt ataaatacct
    21121 gtttcttctt ttccagatac ggcatttctt acttttgaaa ccagtgcttt tacttttgcc
    21181 agaacagaat ctccggttac ttcggtaagc tgttctccct ttttcgtcag gagcaggctt
    21241 cctacctgtt catcgttttt ctgttctacc tctacaatcg cagctcctgt atctggatca
    21301 atctgatgtg cagtgtttga ggatactgta attacaatac cactctgtgg attttcttta
    21361 tatgttcctt ttgtagtctg ttctaatggt gaaataatcg ttgttccatc atacagggac
    21421 tcctcgcttc cctgtcttac aaatccttcc ggtgctttca cttcttcgat tcggtaggta
    21481 gagcacttca gttcctgcgg tgtgatcaga tatccttcct cgtttgtttc gaatacactg
    21541 atcttttctt tcttcggata ctgaacaacc tgttccacat atttcttatt tgtcacatcg
    21601 taaatcttat aagaagcacc agcctttaaa acagtatttc cagtctgtgc atccttcttt
    21661 acaattttca gcaaaaattc aaacgggcgg tcatcaaata ctctccactg cattggctct
    21721 ctgctgtcat tttccacatt tacaacaaat ggatcaatgg ttttcaaatt ctcaggggta
    21781 gtactttcca ctacaacata acttccatac ggcagttccg ggctgactgc atatcctttt
    21841 gtatcagtga tcagttctgg tactggtacg gctgttccat tctcgtaagt aactgcaacc
    21901 tgctctttgc tgaagtcata atttttgaaa tcgcttgctg tatagctttc tccattggct
    21961 ggcttcagtt ttccattttt tacctgtgtc aggtcgctga tcagatatac cttaaatcct
    22021 gctcctgcaa ccaggtctgt ttctgtctgc tctccatctt cactgatttt gattaactgg
    22081 aatgcctgtt tctttacctg ctcttttacc gtaaggtctc ttgtcacttc gacaacatcc
    22141 tgaccttcat aagttactga tacctcgtat ttggtagtat ccagtgtata tccttccggt
    22201 ggagtgatct cttttacata catttctcca aggtataatt ctgaaaattc cagtgttccg
    22261 tcatctccga tgactccctg tgcaatcagg ctgtcctgtt tatacagaac ccctgttgtg
    22321 ccatccggat gcacgatgtc ctcttttgca tacagaccat acacagcacc acgaagaaca
    22381 ctgtccccct gcgctttaaa tgcaagtgtt tctttatcga tctttgcgat tttcaccttt
    22441 ccggtgactc tctcatctgt tgctgttaca gtcattgtct ttccggcagt aacagccact
    22501 ttatatactt ctgtattcag tttgtatcct gtcggagcag tgatctcttt cacgtacact
    22561 gttttcagtg cagaatcaaa ataatcgctg actgcttttc cgtctgttcc ggttgctgac
    22621 attgtcatca gcagattttc acacttctta tcagtataaa taccataaac tgctcctgac
    22681 agtggattct gtgtattgac atctttcttc ataagttcaa tgcgtgtcat attcagccac
    22741 tgtacactga agcttaccgg cgctgctgtt tcgctttcaa atacaccgat atcctgcttg
    22801 gaatctccgg ttgtcaacac cagtgttctc catgtctttc cgacagaacc atataagttg
    22861 ccggaagcat aactgcctgt cagtaataaa tctgcggaca gatagaatgt atctccacca
    22921 taaatctgga tttttccatt tgtaacactt gttcctttgg aaagattatg tgctgtcaca
    22981 ttttctggta cactcagtgt cacatagttt ctgtgatctc cagacagtgt gatatttggt
    23041 gttttctgga tgtttccatc ccgtactgca ttcagctttg tgcttgaaag gctcagttca
    23101 ccttttggcg gttcttcctg tccaaacaga taattgatat atgcgataac ccctgcattt
    23161 acaaggtcat tataattaca ccctgtaaat ccggcttctc ctgcataagc atagctggct
    23221 gcaatgtgtg tatacacata cttttcatct tccgtttttc cagacagata acttcctgtc
    23281 agatctcctg ccccgccata accataataa aggactttct gcagattttt attgctgtcc
    23341 agtacctgtg ctacataact tccactcggt ggggatgctt tctgggactg aaggcagtat
    23401 gcaatttttc cattgactgt aaacaggcaa gtaagataat ttccaatata acttggataa
    23461 taaatagttc tcccctttgt caatgtaact gtggaaccgc tgcttgtagc tctttcagcc
    23521 gcagacaata ccatcacatt tccctcttcg atgtcctctt tcagttcttt gattgcctgt
    23581 gcagatgcat cttcttcgga tgtttcaatt tctacatctg tgtagttctg cacaggcgaa
    23641 tccgaatcat cttcagattc tgtatctgac ttctgtttct ctccaccatt ttcttcggaa
    23701 tgtgcctgtc cttcggtatc cgtcagagtc actttacggg tgatggtata gctgtcactc
    23761 ttatcttttg gaacgaccat ataagtggca atatatgttc ctgctttgtc tgaatgatac
    23821 tctccaccat tctcatcttt gatacttaca agcgtaacat cttctttttc tgcatcataa
    23881 tggattcctt ccatactggt ttccactgca aatgtatcat ctgaaactgc ttttgtgata
    23941 tcatctgctg taactgatgt atctttctgg cttgttaccg cctgttcaga tgcctgtact
    24001 tccgtctgct gttccgagcc tgtctgttcg gccgcaaacg cattcgcact gctcaaggta
    24061 ctgatgccca tcaggacagc cagtccaaaa gcagcaattc tggtctttaa ttttctaacc
    24121 ttcatgtgct tttccttcct attataaata gattttttca gtaaaatacc gcagaagatg
    24181 tttcctctgc ggtacgtttt atcttgcata tcgatttttt tcttttttta cttcctgtct
    24241 ggtacgtttt accgcttcca tgacagactc cgttggaatc tggttatcct tacatccttc
    24301 cagaattcca tccagataaa atttcgatgg ctttgccgga acatctttgt gcggagcgtt
    24361 catcatatat acaaacactt ctttctccac accatcctga ttttttaccc gaatcgattc
    24421 tttcccataa aaacttggga atccttcata aaaatccaga ctcttctcac acgcttctgt
    24481 gattttccat aagactcctt cgacatagct tcctttttcc ggcagaaccg ttgctacccc
    24541 attgcccgga gcccttcccc gaaatgccag ccgataattt tcaagccgga cgttttccac
    24601 aacttccgca gccggacacc tgtacttcat ctgctccaga ttcatgttgc tgccataagc
    24661 gaagtaatat ctatctttca ttttttctca cctctatctg actccttctg tcctttgatt
    24721 tttcttcaga cactcatacg tgcttttatc atatctccac gcaagatcac catccagatt
    24781 tgccagcaga tgttttcgaa catttttata ttcgtctcca attaacccaa gtctcaataa
    24841 aaatgtacga aatgtaaatg ccgggttttc tgatatcagt gtctttttca tctgtgtcga
    24901 tctctgattg attgcctgtg cggaaatggc taatgccagt gtaatatttg ctctcacctt
    24961 tcctgcatgt agtgtgcttt caaaacaacg ccattccaaa gttcccttgg aaaacacagc
    25021 atgaagattc agagcataat aacgggtatg atcataatgg gtatgacttc catccctgcc
    25081 tccataccag acatttctta ctcggtccat tgtgattgat ttatttggta tttttcggat
    25141 tttctccaat acctccggtc gcaccctctg acaataacta tttgctcttc tctcctgcac
    25201 tttcagtgct ttgaacagga tatcttcctt tgcatacata attgtcagtg catttttcag
    25261 actctgcggt gtatgattcg atgcatctac atgaacatgc tgaccacagg actcatttac
    25321 aaaacctcca tgctgtcgca gacatcgtac tacttcctgt aactttccca tttcatcata
    25381 ggataatttc ggtgatacca tttctgttga atattctcca cctgcgtcta caattcgacc
    25441 gtatactttc cgttgtgtat aaatgctccc atcagacata aatttccact tttttccttc
    25501 cagatctatc actgaccaga ctccataagt agtgccgtca taatatgctt ctgttccaaa
    25561 catttcagca acaacttctg cagcacgttg tctggtaatc cctgtcattt cgatttctgt
    25621 gccaaagtat tggtctttta ttccgatcat gcctaatgga ttcctttctc agacactttc
    25681 tgtgtgttaa gttcggagtc tgccttttgt aacaatctgc caattgcctc gataacattg
    25741 acagttactc cgtttccagc ctgtttataa agctgtgcgt cagaagtgct gtctatgatc
    25801 ttatcaatct gccagtcgta ataaccctgc agtcgcagac attccctcgg aactaacctt
    25861 cttattctgc cgtgatacaa gatgccatga cgatcggttg cagtaattgt aaacatcggt
    25921 tcttcgggtt ctttcatacg tctgccgttc tgacgaacat tctcttttgc tggtgttaag
    25981 actgctcttg ctccatcctc tgctaataca ccagaacatt ctcctttgta tttgtggaca
    26041 ccgcattgtc tggtattcag gcttctgcat tcttccgtta ccaccggcgg aggtgaaaga
    26101 tcgacaaagt gcagcgttcc ctgttgaaca cctgttgtca gggtatgtgc gatctggtgt
    26161 ccgactcttc ctctcctgct gttgagattc gcataaccca gatcgatgct gtccccttct
    26221 cttgcaagtt tgtagccgag ctttgtattc tctttgattg gaacacctac atcatataat
    26281 cctgtcttgc cgcccattcc acctgcctgt gatgtcaggg tacagcttag tcctttggga
    26341 ctgtagactc ttttcccctg acttcctgcc cggacttgaa caagagctgc tccatttgtt
    26401 tctggttcag gtaatacttt tccggcacat cggaaatcaa aatatccgac aatatacacc
    26461 cgttttcttg cttgggggac tccgaaatcc ttgctgttaa gcactttcca ttcgacatgg
    26521 taccccagtt cagaaagcgt actgaggatg gtgcaaaacg tccggccttt gtcatgcgat
    26581 aacaaaccgg gaacattttc aaggataaaa tacgagggtc ttttagcttc aagaatccgg
    26641 gcaatttcaa aaaagagagt tcctcttgca tctgcaaatc cttctcgctt tccggcgata
    26701 gagaatgcct ggcaaggaaa tcctgcacag agtaaatcaa aatctggcat tcgttctggt
    26761 tcgattgttc ttgcgtcatt acaataccac tccccttccg tatcaaacag caatcggtag
    26821 ttcttatccg cataagtatc cacctcgcaa tgtccgacac agacaaagtt tcctgcccgt
    26881 cttaagcctt cacgaaatcc cccgattccg ctaaagagat caaaaaattg aatggttgcg
    26941 gctatcaacc tcctttttgc tgataaaaac aggcggcatg atctctcata ccgcccttcg
    27001 ttattctgta tcaattggtt accattctgt tggttcttct gctttggatt cttccggatc
    27061 agtctctcct tctggttcag tttcctcttc cggttcactt tccccttctg gtttgttttc
    27121 ctctttcggt tcactttctc catccggttc ggcttcctct tctggttcac tttccctttc
    27181 cggctcagtt ctttcttccg gttgagtttc ttctgttccc aattctccag ttgtctgttc
    27241 ggattcttca ggctgctctt tgctttgcca ttcgacctct accttttcca gtgcttcttc
    27301 tataatctga attaagaagt ctttctgttt catgtgtttc tttccgatga ctacttttaa
    27361 tctctggaat aattcctctg atacctgtac tgctactgtt cttactgctc ccatgcttat
    27421 ttctcctttt ccttccactc tgttgaaatg ttcttcaatg acctgttgta aaaatttttg
    27481 tgtgctgctt tctgtgagtt caatctcttc tcttactttc tcatgtaagt ccatcgggat
    27541 ctttccgcag atatttttca tctctgccat aacctttctc ctttcacttt tgctacacac
    27601 aacatactcc aaagccgcat ggaaagctat tcacaatacc caccaaagat atcgatgcat
    27661 ttcttgcctt acccctagca gatccaacaa tgtatgtatc gtgcaatcat aggcctcacc
    27721 ccctttacaa attttccatt tgagatagaa aatccatcat ctgatctggt atttcttccg
    27781 atgatggttt cgttttcaat tgttccgtag tgccacatat tcctaattcc cgaacacttt
    27841 cttttatcgt cttttgaatg acttcttcca tccatttccg atcctctttt tccagaattg
    27901 cctgaaccag atactggctt ttctgccctt ctggaacact ttgaaatttc tcccatgccc
    27961 tctggtgttc tggatttttc aggttcgggc ggaacgaata taccgggcgt gtcatctcgt
    28021 ccacatctgc tctacaatcc gttcataccc tgttgcgttg gcatggacgt cctctataaa
    28081 aacggggcgg caaatcgcgt cctgtgcggt cacgtggcgt tttaaaattg cggagcctcc
    28141 ccccataaat acggtgggta ctgcccggag atcaaacccg gattctgtca ccgcagacag
    28201 tattctttct atatatttcc gtccgttttc ctgtatgatc cgtctggcct cctctgccat
    28261 actgcaagat tccctccgaa gcacacgctc aatttgagtt tctgtcacag acagtccggt
    28321 atttctacgc acttgttctg ctgtctcatc aatacatcgg attactccaa gttccagact
    28381 tctgcacgtt gctgcattcg gaacagcatt gtccagcctc ataaggtcaa cggtccagcc
    28441 accaatatca acaagcagaa cagacggttc attcttcaga tactctggat gcagagcaag
    28501 cgcagagtat ccctgtggga acagtttcac atcttttatt gttatctcat ataactcact
    28561 ttcatataaa aatcgcactg gctgctcttt ccgcaacaga tactccctga atccctgttt
    28621 ctctcttcca aaacttgaaa gcggaagtcc aacagccaga attacctctg tctttcgttc
    28681 cgctttccga tgcttgattt cttctgcaat ggctgccaac gtcagcagat aataattatc
    28741 attggacgtt ttatttttta ccagcgtctg tcttcccgta ccgcagacat aatactttcc
    28801 ttgatattgc agaacattct gcatggtata cggctcgtaa tcatacccaa taattccact
    28861 tgggaaagac acttgtcttg ttttaatggc ataatagcca tgatcaattc ctatgattat
    28921 cgcaatcaca tcctttcatt ttttctgttt aacgtaaggt caaaaatgtt gtcactgctg
    28981 caacggatcg ttgcaaaagt gccattcgtg aacacttttc tatgttccag cgtcccaaaa
    29041 cctgcggaaa atctggcttt tttataccac tatcgccaga ggaacactac tgttccttcc
    29101 cagccggtta taaaagatcc ctctcccggc ggcggtccag cctgtccggg ggagggatat
    29161 atcgcaagtt tcctttttga acaattgcct gtctttttat ttttatgaga tgtttttcct
    29221 gcttttgtaa aatgggaaga tactccggtt ctgtcaaaaa cttccgtata taattgccct
    29281 tttttcccag cttgttatca tttttcagca gttctatttc taccatgtaa tgggtatctg
    29341 gatgaaatct cttctcacac aaaatgctgt ccccttttat ataatcagat ttagccgatt
    29401 ctcttgctgc cgccaagagt tcataaatac ttacttccac ctcttatcac ctcccaaaag
    29461 accagaaaaa agcagacctc ttaatcgaag tctgctcctg tcggatatgg taatcctctt
    29521 tcttcatatt ttttatcgat ataatttcga atccattccc tgtattcttc cgttaatgcc
    29581 agttcctgaa attctaacaa ccacttttcc ccttttgctt cgtcatcaag ccatcgatct
    29641 tctatgcact ccggctcatc taacattttc atcatcgctt caaatggctc ttttacatca
    29701 tcaccaaatt ctttccataa ggcgattcca tagttctcca gttcaaaaaa ttctgtcaga
    29761 tatgtcaatc gttccatatc ccccaatgtc attctttttt catctgacaa taatgctcga
    29821 tctcttttgc cacatagatt taaaaatttt ctcttttcca tctccacatc cattatactt
    29881 ttacctcttc ctgttaaaaa taattcctta ttacgaaaca ttataaaatt taaattcttt
    29941 agaatcaata tatatgtttc tgtattacga aactttttat tttataaggg gagttcatca
    30001 ttatgcaaaa gattagacca gatatggata ttggtaaaaa tatccaagca attcgatata
    30061 aaaacaatat gactcaagac caagtagttg caaaattaaa tcttatgggt atctccatca
    30121 ccaaaagtac ctatgccaaa ctggaaacaa atcgcatgaa cattaaggca tctgaattag
    30181 ttgcacttgc aaaactcttt cataccgata tcaatgcttt cttttccggc ttgctatagt
    30241 tcgacattcc ccgactccct gtgtatataa tagcatatta aaaataagaa ccagtctata
    30301 ttcttatcgg atataaacca gttctttttt cttatcaaat aatataaaag tgacagggaa
    30361 ttcagattct tatcttctgt cactcatgta attattattc tattttaaat ttaatgaatt
    30421 tacccattct tgaatttctt tttcaatgtt attttctata taaacgtcat acacattttt
    30481 ccacattatc tgtgtatact gttattttca accccataat tataacttgg tacacgctat
    30541 acactactat aaactgtact ccattttatg aaatccaccg ttccttcaat ccagcaatat
    30601 tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa aggagtacca
    30661 tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc agatagtcga
    30721 cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg tcctaccttt
    30781 tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt cgatccttct
    30841 aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata ctcctgtttt
    30901 gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa attggataat
    30961 ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt tggatggcta
    31021 tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc cgtcggggta
    31081 tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc tcccttatga
    31141 attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt aaagaaaaag
    31201 aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct tttctcagct
    31261 tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta agacgagggc
    31321 gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga tttggcacat
    31381 agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtatttcg atgtccacct
    31441 gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc caccagctta
    31501 ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg tcttcccttg
    31561 tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat aacgtttttc
    31621 ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt tcttcaaatc
    31681 cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct gcaaaagatg
    31741 gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa aaatctccgc
    31801 cgtgagaatg caggtaaagg accggcggaa acggtccttc tttttctgga taagcataga
    31861 gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc ataaaaccgt
    31921 tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat ggagcaccta
    31981 aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc catattttct
    32041 gattgatttg attggaattt ctcaatactg atttctttag tattttctaa tagaaatgct
    32101 ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc aaagccaata
    32161 taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac accgggaact
    32221 gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat gaattgttcc
    32281 gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat attcgaaaaa
    32341 tggatccttc tgaatgctgg tgtccgttca tctatcggat aagccttttt ttcccataca
    32401 tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa tggacacatc
    32461 acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc tcgacctcgt
    32521 ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga aatggtaata
    32581 ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc atggaccatc
    32641 gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc ttcagtccct
    32701 gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat acgcacattt
    32761 ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt caagatcgtc
    32821 aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact tggtgactgg
    32881 atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat caatttagga
    32941 cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt ttttccattt
    33001 ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc atagatacag
    33061 gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg ataatctttt
    33121 ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac attgcttttt
    33181 aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc gcctccgtca
    33241 cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc aattgatgcg
    33301 ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat gcttgcaaga
    33361 atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag taagcatatg
    33421 aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta ttcaagttca
    33481 gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt gatttcttca
    33541 cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta gccattgcgt
    33601 aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt gtttctgttg
    33661 ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca gccagtgtct
    33721 tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat tcataggtat
    33781 ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg gatagacctg
    33841 tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc agcaaatttg
    33901 tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca tcttggtaat
    33961 ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc atatataagc
    34021 catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct tcggatgtgc
    34081 gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat ttttcttcgc
    34141 ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg atattgtatt
    34201 cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg atattgtctt
    34261 ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg aaccctttaa
    34321 gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca tataagcgag
    34381 gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt atttgctctc
    34441 ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc caatcgtcta
    34501 tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg atcgcctcct
    34561 tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg tcccatgatg
    34621 tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc attgttcgtc
    34681 ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc tttgatcaat
    34741 tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac ttcactagga
    34801 cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat aaattcgata
    34861 gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat caatttatac
    34921 acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg tgcttcgtct
    34981 tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc tgaccaaaca
    35041 tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc catatcatct
    35101 tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt gtagattcca
    35161 gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc aaagtcttgt
    35221 gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat atactcttct
    35281 gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt tggtgctcca
    35341 aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc acgatcgcca
    35401 aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa ttgtttagct
    35461 acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc caaccagtct
    35521 tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc gtacaaggca
    35581 ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt tttatagtct
    35641 tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg gacagaagag
    35701 tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga agccaatgca
    35761 gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag ttccgtatcg
    35821 gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg atgcaaaata
    35881 tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt acttgaactg
    35941 ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt tacgagcgtt
    36001 gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt cttctgtttt
    36061 ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg tccacgtgac
    36121 tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg tttggatgat
    36181 catcagataa atccctacca tacttaatat caacactaat gagtaatgaa gatgtgagaa
    36241 tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat aatgagccat
    36301 gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat acaaagcgat
    36361 cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc cgatggcgcc
    36421 atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg tgaagtacgt
    36481 tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg caaccatcgc
    36541 atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac cgccccacag
    36601 aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt tatctaatga
    36661 ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc ctagcttttt
    36721 ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa acaatcccat
    36781 tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat aggtgtcgaa
    36841 aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct cgaaccctaa
    36901 gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac gaattgccgg
    36961 caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg cagcttcata
    37021 caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc agcccaactc
    37081 tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg ttagaaaact
    37141 gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt caacattcaa
    37201 aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga tgtaaatgat
    37261 cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta aagacaaaat
    37321 gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg tatttcttaa
    37381 taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac cgacccaagg
    37441 acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga gtccccccat
    37501 aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca catataaaaa
    37561 tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt cttctttttt
    37621 cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc cctccttagt
    37681 tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt acgtatataa
    37741 tcatttttac cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta tacaaaacga
    37801 taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt tttctatctg
    37861 acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa ttgaacatat
    37921 agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa aagaaagtct
    37981 ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg ctagctttga
    38041 tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca caagaagtcg
    38101 tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat cgctacattc
    38161 tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca ggtgtcggaa
    38221 cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga aagtatttgt
    38281 ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc ggggggatga
    38341 ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg gccatgatcc
    38401 tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc caagccttgc
    38461 cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt gtatttttca
    38521 aaattatgct gccattagcg ttaccgtcta ttgctactat ctcgcttttt tatgctgtta
    38581 cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct aaatggccga
    38641 tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca gaaagtagtg
    38701 ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt attgcaacag
    38761 tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga gcactcgtag
    38821 gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca gaagtggtca
    38881 gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt gaaatttcgg
    38941 cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg aaagagacgt
    39001 tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc cacaggagca
    39061 ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc ttattgtcga
    39121 tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga cttttgtcac
    39181 agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa tactagggtt
    39241 attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct tgattcacaa
    39301 atattttttt cgatattctc ccggcgtaat cgattccttc tttttaaaga aacgaataaa
    39361 attttgcgga tttcgatatt gcaatctttc tgcaatctct tttacggaga ggttagtctc
    39421 tttcaaccat ttttttgcta cctctaaacg atgattttga acaaaatcac caaatttttc
    39481 tcctgtttcc tttttaaata cattacttaa atagttaggg ttataatgca acctatcagc
    39541 gatcacatct aaagacagat cacgatcata ttctgtgtag acgatatgca taatattctc
    39601 cgacaaattt ttaaattcat gatcagtgat tgcttgagag cattctgtga ttggcagaac
    39661 taattgttct aaaacaagtt tttctagttc tttgggatga taattgttca gtgcttttac
    39721 ataaatttgt ttcatgttat caaagttttt tgaatctact cccaataact gtcctaattg
    39781 aacgagttca ttgatcaagc ggaccgaaac tacttcccga ctcaaaggat ttttattttg
    39841 tgcaaacaat gcatcaatca attcatgtac taaattcttc acttcctgat ttccagaacg
    39901 aatggcttca aacaatcgat tttgttcttc tatcggatat ctaataagtg ttttttcatg
    39961 agcagcagga acgatttcct gataaaaaat aattgaactt ggaccagtat ttacacgata
    40021 atacagggct tcttttgccc gatcaaccga ttgcttgctt tcattcagcg tttcgaaccg
    40081 gttactgatg cctatactga tagttaaatt caagtattct tttacacgtt tttgaatgag
    40141 tcggcaatac tccatgactt ttttgcttgc ttctgcatca tctttatcca ttcgataaat
    40201 agtggcttgc atttcttcat tcaaaacgat cggcagcatt cgattttcct ttggcacaat
    40261 ttccgtgatc atgttgttga tcgctaaaag tagcaaatca cgctccccac cgtgactatc
    40321 ttctaacaga tcgatttgaa ccaaagctgt ataatagcag tcattttgag aactataacc
    40381 aaattgctgc aaccgctgat ctagttctcg cctttctact cgatttctaa ataaactaag
    40441 tacaaatagc gtttccagct gtggcttttg ggtaactaag tgtgcactta acgcttcttt
    40501 ttctcctatg atccgactga ctgattgagc gaccaaatct agttctttcc cactgaatcc
    40561 ttttgattta agattcagac gttcctgtat ttgagaaatc ggacgggcaa atctgtccga
    40621 aaataagtag gaaagaagac caatgagtcc taccagtgtt aaagacactg caactagccc
    40681 gattctcaga ttgcggatca ttgcgccaat tgcactagag tcaacttcta atctgtaaat
    40741 ccaattatta taatctgatt tcagtactat atacgtttta tctttttctc ttatcacttt
    40801 cagctgattg taatctagat ccacgtccgt taatacagtt ggttgctctt tattttttac
    40861 tgtctgagta gtaaaaaggt tgccaacttc gttttcaata gaaagtatgt cttctccact
    40921 ttcaccaaca acttgttcta ttgtatgttt ctctatcgta gcaattccca atgcatattt
    40981 ttctttttca aacattggca aagaaatcac catttcgatt cctttgtttt tcttttgcca
    41041 aaataaatta tttttgttat ttatgtactt ctgttgatac ttttcaattt cctcttcggt
    41101 cagttgactc aacgatccat tgattactcc ccatttacca gccaaactga ctaaatcata
    41161 attattatca tccataacaa tcgtttctat aaaatttagt tctttcctga tttcagaata
    41221 agtggagtaa tctgtatatg tcagcggatg cttgaatatg tccgagaaat ttttggtcga
    41281 accatacgta gaaaacgaat attcaatcgt tctaattttc cattcaacgt tagttttaac
    41341 ttgcctgagc aaattctttt tgtctgttaa ataactcgat tcgatcgatt cataggtatt
    41401 ctggtaaatg aataaactaa atcctatgac aagtacggct cctaataaaa acatcggtaa
    41461 aaatatttta taaaatactg tacttttctt cattttcttc ctctcctcct tttaaaataa
    41521 aaggctttca tttttctgta aaattgatac tcatctaatt aaatgagcat tccccgcttt
    41581 caagaatagc ataattaaaa ataaaaatcc aagtaatttt taacgtgtgc aatcttttca
    41641 tatcaaagat ctattgactt atcgtagtta tcgtaatatg atgaatgcgt taacaaaaag
    41701 tgaaaaagta aggaaaatga tggaatggta acgatcaaag atgtcgctaa agcagctggc
    41761 gtttctcata cgacagtttc tcgtgctttg aataacagca gtttaataaa aaatgaaaca
    41821 aaacaaaaaa tacgacaact agctaaggaa atgaattatt cgccaaacta tagtgcaaaa
    41881 ggtttagtga atcaaaaaaa atatctaatc ggactatttt tctcaagttt gcataaagga
    41941 acttcatcaa atttcctagc agattccatt gcaggcatcc attcagtatt agacacaaat
    42001 tacagtttat ctgttgaaag tatcgatgtt gttgatttaa cagaaatcaa cctgcaacgc
    42061 tacgatggac tgatcgttat gagtcaaagt gatgatgatc agcctttcat cgactatctc
    42121 aaagaaaaag aatttccttt cgttgttgtc aatcgtcaca tcaatgatcc agcggttatc
    42181 aacgtcactg ccgatgatgc ggccggtgtt atgcaggcag tagattacgg tattggatgc
    42241 ggacatacga agatcgctta tattggcgga aagaaaaatt ttcgttcgac aaaagaacgg
    42301 gaaaaagggg tattgaaaag tctaaaaaaa gcgagcatcc ctgtcaatcc agcctatttt
    42361 ttaacaggag attacagctt agaaagcggc ctccaaaaca tggaatacct attatcactt
    42421 tctgacctgc ctactctggt tttttgcggc aacgacgaca tagcgattgg tgcattgaga
    42481 gctgtacatt tagctggcct ccgtgttccc cagaacattt ctctgatcgg gttcgatgat
    42541 tctccagtcg tctcctatct caatccgccg cttaccaccg tacgtaagcc tttgaaagaa
    42601 atgtgtcaag aaggaacaaa gcttttacta gatttgatcg aaggcaggac gatcgaacaa
    42661 tctaaaattg agctctccac cttgttgatc cttcgtgagt cgatcaatta cttgtaaact
    42721 acaagtaatt ttatataaaa atgttaacgt gtgcataaca cagaaaggaa gtttatatga
    42781 aaaaactgac aaaagaatta gtgaaaaaaa acgaagcacc tgtaaaagtt cttcagtttg
    42841 gtgaaggcaa ttttatgcgt ggatttattg attggcagat tcagcagctg aataaccaac
    42901 aattgtttaa aggtaatgtt gcaatcattc aacctttaga acaaggtctt ggaaatatga
    42961 tgaaccagca agataattta tatacggtca ttttacaagg actcagcaat ggcgaaatcg
    43021 ttgatacttc cgagattatc accgtagtag aacaaatgat caatccttat gatgaatggg
    43081 atgcttatct atctctagca gaaaatgacg acttggccta tatcgtctcc aatacaacag
    43141 aggcaggaat ccaattcgtg gagactgata atttaacaga tactccccca cagagcttcc
    43201 cgggcaaact gacagccttt ttataccgac gtttcaaatt aaacaaagct ggctttacca
    43261 tcattccttg cgaactgatt gacagaaacg gcgaacgtct aaaagaaatc attttacgct
    43321 acgcagaaaa atgggattta gaacctgctt ttgttgagtg gttagaaaaa gaaaatgttt
    43381 tctgttgtag tttagttgat cgcatcgtcc caggttatcc tcgcgaagat gcaggtgttt
    43441 tcaatcaacg gcataactat gaagatcaat tgatggtcaa agcagaacca ttcatgcttt
    43501 gggtgatcga agggcctaaa gaattggaga atcaactacc attcaaacag gctggcttga
    43561 atgtcatcat cacagatgat atgactcctt atcgtgaacg taaggtgcat ttgcttaatg
    43621 gaccgcatac agcgatggtc cctcttgctt tgttggcgga attggaaact gtagaagaag
    43681 taatgaaaga tcctttattc tccgcatatg ttgatcagtt attcaatcta gaattgattc
    43741 ctatgctttc tttacctaaa gatgaattag ccatttatgc tgaccaaatc aaagaacgtt
    43801 tcttgaatcc ttttgcccat cacaaattag aagcgatttc attaaatagt gtttcaaagt
    43861 tcagcacccg tctgcttcca gtgttcaaga agtatatcga agaacaaaat caggtaccac
    43921 cgttaatcac tgtttcacta gctgcgctac ttcttatgta tcgaggcgat caagtaaagc
    43981 cacatgatga tgaaaagacg ataagcgaat ttactgatgc ttggtctgat aatggaacag
    44041 ctattcctcg attattgcaa aacgctgcgc tttggggaga agatttgtct caaataccga
    44101 atgtgacaga tacagttcaa gaaatagctg caaagatcga tcaaaaaggt gtccggtcag
    44161 tattacaaga aatgaaagga acgatggtcc atgcttgaaa ccctgcaacc aacaatgaaa
    44221 ttaaatcgac gagacagtgt ggtcgtcgca ttaaccccta ttccaaaaca aacacaactg
    44281 acgattgatc agcaaacgat caccactctt gaagatatcc cgcaaggtca taagattgca
    44341 ttgggtgatt tgaaagctgg agacaacgtg atcaaatatg gctatccaat cggtcatgtg
    44401 acgacagacg taactgccgg tcaatggctg cacactcata atgtaaaaac caatctttct
    44461 ggtgaattgg attatcgata tgaaaaagat gtccatccaa gtcattacct ttttgagaac
    44521 cgaacatttc aaggttactt gcgaaaaaat ggaaaagtag ggattcgtaa tgatttgttt
    44581 ttagtaccta ccgttgggtg tgtgaatggc attgctgaac tgatcgtcaa acagttcaag
    44641 gaaaaacatc cagatctggg acagtttgac catattacga ttttaaagca tccttatggc
    44701 tgttcgcagc ttggaaaaga tcatcaaaat actcgagaaa ttttagcgga cgcagtaaat
    44761 catcccaatg ccggaggagt tttagtattt ggattaggtt gtgagaacaa tacagttcct
    44821 gaattcaaaa aaattttagg tgaatatgat gaagaacgag tgaaatttct agtggctcaa
    44881 gatgtttacg atgagatcga acaaggtgtg gctctattag aagagctttt aacagcggcg
    44941 gaaaatgacc agcgcatatc agtcccgctc tctaagttga atgtcggttt gaaatgcggc
    45001 ggttctgatg gtctatcggg aataactgct aatcctttac ttggtgcatt ttctgactac
    45061 ttgattgctc aaggaggcag cacgatctta acagaagtgc cagaaatgtt tggcgcagaa
    45121 caagtattga tggcacgagc ggaaaatgaa gaagtttttg actcaatcgt tgacttgatc
    45181 aatgacttca aacagtattt cttgtcttac ggggaaccag tttacgaaaa cccatctcct
    45241 ggtaataaag caggtgggat cactacatta gaagataagt ctttaggctg cacgcaaaaa
    45301 tcaggaagct ctcctgtcgt tgatgtgcta caatatggcg aaaaaatcag aaaaccaggg
    45361 cttagtttat tgcaagctcc cgggaatgat cttgtagctg cctctgctct tgcttcatcc
    45421 gattgccagt tggtcttgtt taccacagga agagggactc cgtttggctc atatgtccca
    45481 acggtcaagg tatctacgaa tacaactctt tttgaacgca aaggtcattg gatggatttt
    45541 aatgcaggcg tcttattaga acaaccaatg gaagtaatct tagaatcgtt tgttcaaaaa
    45601 atcatcgctg tagctagtgg agaagaaacg aaaaatgaac aaaatgacgt tcgagaaatt
    45661 gccattttta aaaacggagt aacactttga ttttcataaa acagtataaa aagaaagaag
    45721 gaagatttat gtttttatct gatgattttt tattaaaaga cgattgggcg aaaaagcttt
    45781 accattccta cgcaaaaaac atgcccatta ttgattatca ctgtcactta gatccaaaag
    45841 aaatttacga aaacaacaat ttcagcaatc tgacagaagc ttggttagga ggcgatcatt
    45901 acaaatggcg cttgatgcga gcttgcggcg tgcctgaaga aaaaatcaca ggaaatgctt
    45961 ctgattttga aaaattttta gcttggtgtc aaaccgtacc aaaaattatc ggaaatccgc
    46021 tttacagttg gtcacatctt gaattgaagc gtttcttcgg tattgacttg ttgataaatg
    46081 aagaaaatgc agagaaaatt tggacacaag caaatgaaat tttggcagct cctgattttc
    46141 gccgtcggga aatcgcaaaa aaatcgaatg tagaagtgat ttgtacaaca gatgatcccg
    46201 cagacgactt gcactatcat cagttgcttg ctcatgaaga accagatttg aaggttctac
    46261 cttcattccg tccagataaa gcactcaata ttgaaaaaga aggatttaat gcatgggtca
    46321 cactactgga agaagcagct gaccaaacga tcaccaccta tcaagaactg atagaagtct
    46381 tagggcagcg gatagaatac ttccatcaac atggatgtcg tgtttccgac cacgctttag
    46441 acatcttacg ctataaagaa gcggatgaaa cagtattaga agagattttt caaaaagcac
    46501 gagctaacaa actattatct tcagatgaaa tcgatgctta tcgtacagaa acactgatac
    46561 atttgaccca cttctatcat tctcataatt ggacgatgca gctgcacatc catgcttatc
    46621 gtaactgtaa tacacaaatg ttggaaaaat tagggcccga taccggctat gatggaatga
    46681 atgatctttc tttgactatt cctctgcaaa aattattgaa ccgtgcagaa gaaacggatc
    46741 agttgccaaa gacgatcctt tattcattga atccgaatga ctatcctgcg attttagctt
    46801 tgatgggctg tttccaaaaa gaacagaatg gaaaactgca gttaggttct ggatggtggt
    46861 acaacgatac tcgagctggt atgcgcgatc aattgacaca atttgctgat ggtagtgctt
    46921 tggggaattt tgtcggcatg ctgacagatt caagaagttt cctgtcctac actagacatg
    46981 aatatttccg tcgtgttctt tgtgaatttt taggcgaaat ggtcatgcga ggagaagcac
    47041 ctgaagatga acaactttta ggaagtctag ttcaagcgat tagttataca aatgctaaaa
    47101 attactttgg cttttttgcc taattgatta atagtaacag taacgaatca aaaacaggat
    47161 agtgaaataa cggaatccgt ttatccacgt tttagagaag aagacggacc aaatttgctc
    47221 cgtcttcttc tcttgctgtt ctgcttcatt gcctataaac ctttatgaaa gaagagataa
    47281 catgcctact atccatattg ccggtgattc tactgcggca gaaaaagatg aaaacaagcg
    47341 tcccgagaca ggttggggag aaaaaatctc tgcttatttt tcacccgaga tccagatcaa
    47401 taaccaagca aagaatggac gcagcaccaa atcatttatc caggaaggaa ggttagcccg
    47461 tctagcttca cagtttcagc cgaatgattt cttatttgta caatttggac ataacgacca
    47521 gaaaatccaa gaagaaagag gtacgcttcc cttcggtagc tatttaaaaa atctgcagat
    47581 attttttgaa tgcgcaaaaa aagcgaacgt ccaaatggtc ttactgacat ctgtgacgcg
    47641 acgagattac ttagaaaacg gaacattgaa tccagatata cttggagact atccaaaagc
    47701 gatgcgtgca tttgctgaga aacaccatat tcctctttta gatgtgttct caagatcaca
    47761 agaattgttc cagaccttct caaaagaaga gacaaaaaaa ttctacctgc atcttatacc
    47821 tgatacttgt aaaaattacc cagaaggact aaaagacaat acccacttta gtccagaagg
    47881 cgctgataaa gttgcccgaa taattgttga attgataaaa gaatcccgtc tgcctcttgc
    47941 taattattta caaaaaggag tttgagatgt ttccattaaa tttaggcatc cgtgcccacg
    48001 atttagatgt tcgaaatcgt gaagaaatcg ttcaaaaagt atcacagctt cacttgtccc
    48061 atatccaatt tgcaccacaa aaagcatttc ctgaacaact taaattaact gacatgacac
    48121 ttgggagtgc tgcctatttt ggtgaatact tccaaaaaaa caatatcgaa ctttccattt
    48181 taggatgcta tatcaaccta tccagcaaaa atcaagaagt tcgtgcacaa gcattggcaa
    48241 ctttcaaaca gcaaattgct ctgtgctctg cctatcaagc caagatgatt gctacggaaa
    48301 ccggtagtgt ctctgtcggt tacacaaaag aaaattttac tgaagaagct tatcagatcg
    48361 ccagaaattc tatcattgaa ttagtcgctc atgcagaaaa ttttggcgta actgttgctg
    48421 ttgaagcagg gatcaatcat ccaatctata actatcagtt agctaaacgt ttaatcgatg
    48481 aagtagcttc accaaattta aaaatcattt tagattgtgc gaatttgatg cataaagaaa
    48541 atcatgctga tcaggaaaaa gtgatccgag gtgccttgga caatttacat ggttatatta
    48601 cagcgcttca tctcaaagac tatatcatgg aaaatggaaa gatccgcatg gtacctgttg
    48661 gcaagggctg tttagatttc cgcccggtac tgcattatat caaatatgag cgtcctctaa
    48721 tgtacgccac tcttgaagct actcctgaga tttatgttcc tgaagcaata gaacacttgc
    48781 agtttattta ccaagattgt taaggagaag attatggagg aacgaataca gaaaagttat
    48841 caaaaatatc atgcaaatgc ttttcagaca ttgctgcatc tttatcaggg aaattattgg
    48901 cgttttgcca tgtcaggact cttttttatt atcaaacatc ttccatcttg gttaatgccg
    48961 atcgctattg ccaatgtaat caatgcagct tcatcgaaag attcttctca gttgcaggcc
    49021 atttatttca acgctttttt gatggcttgt ctgattggtc aaaatatatt gacgaattat
    49081 tttcatgtaa aataccatag cacttcgatc cgtcaagtag aagctggact aagagcgacc
    49141 cttatcaaga aaatccaaga actatctatt ccttatcaaa aagaattgca atctggacga
    49201 atccagtcaa agatcatccg tgatgtagaa gctatccaaa cgttgtcctc tcaagtattt
    49261 gtcagcggat taaatatcgc tgtcaattta ttcgtagctt taggtatcac gctttataaa
    49321 agtcctgttg ttttcatgtt tttcttattg actgttcctc tcgccgcatt cgttactctc
    49381 tttttccgta aaaggatcag acagtccaac tcagacttca ggcaagaaat ggaagagact
    49441 tctgcaaaag tgatcgaagt gatcgaatta gcaccgattg ctcgagctca tgggttagaa
    49501 aaacacgaaa caactcgctt aaataagcat tttactcaaa tctatgaaaa aggattccac
    49561 ttagatatcc tgcaatctgt tttcggatca gtcagctggg ctagttttca gatatttcag
    49621 ctgatttgtt tgattttcac tgggatatta gctttacaag ggcgcatcca acccggcgat
    49681 gtcgtgatgt atcagacgta tttcacgacg atcgtcaatt ccgtttctgg aatgatcacg
    49741 ctgattccta cgatttcaaa aggcatggaa tctatcactt ctattggcga actgttgatt
    49801 gctggcgatg tagaaactta tcacggtaag aaaaaaatcc aacatttgga tggaaatttt
    49861 cgttttgagg atgtttcttt tctttatccg gactcactca aaccgattat cgatcatttt
    49921 tctctagagg tgaaagctgg agaaacaatt gcttttgttg ggccatctgg ctctggaaaa
    49981 tctacactcc ttaacttatt gattggattt atccagccga cttcaggaaa aatttttcta
    50041 gatgatcagc ctttggatga actggatatg cgaagtgtgc gaaattattt agccgttgtt
    50101 cctcaaacca ctttgttgtt ctccgcttcg atcaaagaga acatcactta tggcttgaaa
    50161 aacgtatcaa aagaacgttt agatgaagtc atcgaagctg cgcaactttc ttcactgatt
    50221 gaccagcttc ctgatggtct ggatacctta gtaggcgaac atggcaacaa gctgtctggt
    50281 ggccaaaagc aacgtatctc cattgcacga gcgttgatac gtcagccaaa aatcattttg
    50341 ctagatgaag caacctcagc tttagataac cagtcagaga aaaaaatcca gcaagccctg
    50401 aattacctga cagaaacacc cactactttt attgtggctc atcggctttc aacgatcaaa
    50461 gaggcagata aaatcgttgt gatcgatgaa ggaaaaatcg ttgaaatagg tacttacgaa
    50521 aaactgttag ctcaaaaagg ctatttctac cgcttgaaaa atgaatgaca aaaaatagga
    50581 gctatgacaa gacttttgtc gtagctccta tttctgaata aacggtgttc tcctgccaac
    50641 tcctcggtca caagtcacaa tagtcatcaa acatgagaag ctgttttttg aaattgttcc
    50701 ttgtaactca gagttaaacg gtaggctcac gacctcttgg taaatggtgt tcaagagaca
    50761 gcctatcggc aataagaagc aattttgtca tcgctcaagg ccgaacgtct ctttcacacc
    50821 ctctgttgtt tcataaaata gttttattcg ctgtaaaggt cgcgaactgg tgtcatgatg
    50881 atacggttca agtcattgat gattgtactg aagccttgct ctttttgcat caagtcattg
    50941 attacttctt ctgattggat tttcacagcc atttcttgtg ctttcattgc atcttcgtcg
    51001 ctgaactctt gtccactcat ttgtttttct tgtaattcga tttgcatcgc ttggaagtct
    51061 ttgaacagtt tgtgtgcttc ttcgtttgct tgtactttct cgtacgcttc tttcaacgct
    51121 ttgaattctg gtaattcacg gatctcacgt tcgatttgat tcgcgctatc gtagatgttt
    51181 gacatgttat gccctctttt cttttagtag ttactctcta ttgtaacatg gggttattcg
    51241 ttttcaaagg ggctactctc cttgatattt ccgccagagg tctttagccg cttcaccaat
    51301 tccctgaacg ccttctttca ccatatcgcc gaagtttttc agcccttctt ttgcttgttc
    51361 accatattga ttcaagttat cttgccaatt tccagactct ccattagaat ttcctgtgtt
    51421 tccaacttga tctgcagcta ccacttgtcc tcctgtggca taggcatctg ctacactaaa
    51481 tcctgttttt gaagtatacg gaaggatgcc ttctgccgct gatttgaaga ctttcccgac
    51541 aacatttcca cttgtccctt ccaaatagtg cagcttgctg gattcttcgt aacccatcca
    51601 tgtcgaaatc actacgtctg gcgtgtaacc gatcatccat tggtcatttg cttttgtggc
    51661 atcgaaatta gtttctgtgg ttcctgtttt ccctgctatc gtataacctg caggattcgc
    51721 agcagccgct gttccatttg aaaatgtacc aagaagcatg ctggtcattt catcggctgt
    51781 ttcctttgat atgacctgtt ttggtttttt gtttgtgtta tcgacgatca ccgctccagt
    51841 cgaatcaacg attttcgtga taagatgcgg cgtatacatc tgaccatcat tggcaaatgc
    51901 actgtaagca cccgccatga tcaatggaga tactcctttt tccagtccac caagggctaa
    51961 gccataatac ttatcagatt ttgctaaagg caggccgaat tcttctgctt tatcatatcc
    52021 tttttgtaaa ccgatctcat gcaaaagcca tacagtaggc aaattcaaac tttgtgcaac
    52081 agcttgatac atcggtactt ctccttggta tgtcccgtca aaattatgcg catcgtaata
    52141 actttgcggt tcgtctttca agatactgga aggcttgtat ccagcttcca gtgctggtgt
    52201 atatacgctt aatggtttga tcgttgatcc tggtgaacga cgcatctgtg tagcaaagct
    52261 gaatcctcta aagacatgat ctcctcttcg tcctactagt gcttgtacac cacctgtatt
    52321 cggatctaaa gctactgaag cagattggac catggcacca tctgaagcat ttgctgggaa
    52381 taatgcactg ttttgataag tagcttctaa ttgcttttga taatcctgat ctaaagctgt
    52441 atagatttta tagcctttat tcatgacatc ttcttcatca agaccatact cgttaactgc
    52501 ttcatcgatc actgcatcaa aataataagg ataacgataa ccagaatctg aattatcgta
    52561 tgtgtcatta agatacgcgc tgatatctgt ctggctttct gcttgttctt gttcttttgt
    52621 gatttttccg ttttcttcca ttacacttag tactgtgttt ttccgatcat tcgcattttt
    52681 tgggtgatcg atcggattat aaataccagg acctttcagc attcctgcca aagtagcagc
    52741 ctcagacagc gtaacattcg ttgcatctac tccaaaatac tttctggaag cgtcttggac
    52801 accccatacc ccgttgccaa aataggcatt gttaagatac atggtcaaga tttcttcttt
    52861 gctgtacttc ttttcgatct cgattgccaa aaataattcc ttcgcttttc gatcaaacgt
    52921 ttggtctaat gtcagatacg catttttggc aagctgctga gtgatcgtac ttcctccgcc
    52981 ccctgatgtt ccgccagaca agaccattcg gactgctgca cgggcaatcc ctttgatatc
    53041 aaacccgtga tgctcgtaaa aattcctatc ttctgtcgag attactgcat cttggatata
    53101 gggagaaatc gcgtttaatt ctacatatgt tcctttttgg gcgtacagtg tgccagcttc
    53161 ttcgtttttc ttatcataaa tgactgtcga ttggcttaat cctgatttta acgtttcgac
    53221 atttgcttgt tttgctaaaa taaagaggta gatacttgtc actaagacac agatcaaacc
    53281 aatcagcaaa atcaatttgt tcacatgata ttttttccag atgcgctttc gccatcgatg
    53341 gaattgtccg agatatggtc tgatccaacg ccaaaaactg atcaaagcag cttttatctt
    53401 ttctaaaatg ctttgaaaat ccatttgctt cttctcctgt ctatttgatt caatcgtctt
    53461 attttaccat agtgaaagag tgttttcttc ctgcttaagt tgtagttcaa tatgtttttt
    53521 tacgttagtg caagtagagt aacataagaa tgaacagaaa aatgtaagtt ttttcttaaa
    53581 tatgttttaa aaatggtaca attagtctgc gattgcactg aaaggagtac acttgtgaaa
    53641 atcacgatca ctttacctat gaaccatcct gaaacaacaa ttcgcgactt gcttgaaaag
    53701 gaatggttag ttcctagaaa ggttcgacac tttttgcgta tacgtaaaaa cgtttggatc
    53761 aatcaacaac ctgctttgtt ccatcatgaa gtaaagggca atgaccagat cacattgtta
    53821 ttcgaagaaa cagattataa ttatcaagaa gttcaattag gagatgcgaa gcacatccgt
    53881 gttttatatg aagatgagca tttgattata gtggataagc ctgcaggaat gaagactcat
    53941 ccaaatgaac cgacagaaaa cagtacctta ctcaatcatt tagctgctta tctcgacact
    54001 aaacagcaac gaccttacgt tgtccaccgc ttggacaaag aaacaagcgg tgccgtttta
    54061 tttgcaaaaa atccatttgt cttgccgata cttggaagaa tgttggaaca aaaaatgatc
    54121 tatcggagat atcaagccat cgtatgggga gaaattgcca aagatatgac catcaccaat
    54181 aaaattggac gagatcgcca tgatcggaga aaaagggtcg tagatgataa aaatggtcag
    54241 agcgccttga cacatgtcac tgtcaacaaa gtactttctg aacaaacaca agtctattgt
    54301 gttttggaaa ccggacgtac acaccagatc agagttcatt tagctcataa gggacatccg
    54361 atcgttggag accctctcta ccaaaataga acagctgaac gactgatgct ccatgcatta
    54421 gaactgcaca tgatccatcc atttactcaa gaaaaaatag tcggtaaagc tttacctgga
    54481 ttatgggaat agaaataaaa aagaacagag tcttatcagc agataatgat aagactctgt
    54541 tctttttgtg taatttgtct gcttactttt gatgttcgtg ttctgctaac agcaaactac
    54601 cgataatccc actttcattc ggcagtcccc aacgaacgat atattcactt acttgaggtg
    54661 tttccatata accacccatc aattcaacaa attgctgacg gattttttga aggagatgat
    54721 cctgattcat taccccaccg cctaaaataa tcttttcagg tgctaacgtc aacgtatagt
    54781 tgatcagtgc ttgagcaata taataagcct gaatagccca aacaggatga tcttgcggca
    54841 gatcttgccc tttgatccct gtgcgtgcct caatagacgg tcctgctgct aatccttcta
    54901 aacaatcttt atgataagga caagtacctt cataatggtc atctggatgg cgtttcaccc
    54961 aaatatgtcc catttcagga tgtgcgatcc ctgaaaaaat ttcttcattg atcacgacac
    55021 ctgcaccgat tcctgttccc acggtaaggt aaatacaact attttttcct tgtgctgctc
    55081 cttgcttcaa ttccccatat gcagcagcat tgacatccgt tgtccaagca gcaggaatat
    55141 tgtagcgttc cttgattgtg ccaagaaaat cgaattgtgc ccaccctttt ttgggagttg
    55201 ccaaaacgta tccgtaatta tcttttgttt tgtcgatacc aatcgggccg aatgagccaa
    55261 tcccgattcc cttcaattca taacgatcaa aaaattcaaa tacctgatcc aatgtttctt
    55321 ctggtgttgt ggtagggata cttaattttt ctaaaatttc tccaccatct gaaaccgcac
    55381 acacaaattt tgttccgcct gcttctattc cgccatacat tcgttttcct cctcgaatcc
    55441 gattgtccga atctcataag gttggattat tgggacaacc gcttgcttcg ttggttcttc
    55501 aagcagattc aatatgacag tttttccatt gtctttagtt atttccaatt tttctaaatg
    55561 actagacata ttgaaacctc ttactactac tttatcacta aacttgctgc gtttcaaagc
    55621 tgtaattgca aacgtttcac tttttatcgt taaatattgt tgttttgaaa tcaatgttcc
    55681 ttcatgatgt ttgatttgtt gagtggaaaa aggaatctgt gctgcgtatg catgcaaata
    55741 agtagagaat ttttcttctg gtccatgtag ttcaatgctg taattgaacc gatgctcacc
    55801 tagacactgt gcttctggtg ttggaaaata tccccaatcg cctagttctc ccacactgcg
    55861 aagtagcgtc actgcgattt gtccgtcttc tgttacttca tattcattca aaccaaagtt
    55921 tccgactgtt acaccgtact cttcatcatg aatattgaca aatgcttgct gatgttgcgg
    55981 attcgtgggg ttttcccaac ttttagacac atggtttggg cgcttcacca cttcaaaaat
    56041 actatctgct tcgtgatgtt ccacatgtaa ctttgtagga aacaagacac gtaagcgatg
    56101 atctttcatc tgattgtcga gagtcgtttc aaagtcgatt tttttactat cttttctgat
    56161 tgtcatgatt gtttttattt gaaggattcg tttttctttg gatcgttctg cttttcgata
    56221 acgaaattcc acaaccatct gctgttcttt ttctaaacgc tcgtctgcag aaacagggat
    56281 ttccatttcc tgtagaatac ttatttttgc tatttccggt gtatctacta atacttttac
    56341 ttcagagttt tctttgttac tggataggat tggctgatca cagaaaggtt gtttaaagat
    56401 atactcattt cctatatcgc cgatatcttc aaaaatatgc agattattca actttcgttt
    56461 gtttttgcga tcttccattg taatggtacc atttttttca atcgtcaaat gtagagggcc
    56521 gttttcgatg attcgtccac tctgatgaat cattgtttcc ttttcttctg ctttcgcttc
    56581 cccttctaca agcgcgaatg tttcccaaga aaatgctggc atgttttcta atggtaaagt
    56641 cactttgatc attcttgcca tatatggcac acggaaacgg tctttcggca aatcataacc
    56701 aaattggaca gtttcttcag ataaaatagc tggtatagtg gccccacttt ttgtttctac
    56761 atgatactta cgtttaggca aattttctaa ctcttgatat agttgttcag gtattccttc
    56821 agcgaatctt tttctttcta attcgatggt tatttctgct tcaccagttt tatccattcc
    56881 tgcagtattt acaatgacaa atggaaagct gtcttttggg aatccggtcg tatcgattgc
    56941 tgcggtcagc tgttctaagg aatcttgtgc aagatacttt ccaacttcat ctgctttttc
    57001 aaaacgaggt atcatttccc gatggacaga atcgactgaa caaccgcaaa tactgtcgtg
    57061 aggatgattt tgcattaatg tcttccaagc ataatctaat tgatcatggg gatagtttcc
    57121 actaacttca taagccatcg tcgcaagtgg ttctgtaata ttttctagct gtcgctggac
    57181 cttggtattc cattgtttta agtaaacccg agcagatgct gtattggcta aagtatacca
    57241 gccatccgtt tcttgacttg tcaattctcc ttctactgag cctaagtctt ctggcacatc
    57301 tttttgaact gcttccagat agtcagtaaa attagaatga ataaactcgt aatctggaaa
    57361 cagttcattt gcgagatgga ttgcttttga tatatctttt tgtaccggtt gatgatcaac
    57421 accattcatc atcaataagt ggttcgtaga ggcatattgt tctgcatccg ctaatttttg
    57481 cttccaaaag gcaagagctg cttctttttc tactggaatc tcattcccgt tgctatacca
    57541 attggcaaat agcagaccaa agattgctgt ttgatcaggt cctttccacc acatctctga
    57601 gtattgggaa gaatagtttt ctgcttccaa aacttgatta tcaaagccaa ttggttttac
    57661 accacggcca aacgctgctg ctgaaatacc agcttgtttc attaattgag gcgtctgtcc
    57721 catatttcca aatgtatcgg gaaaatagcc taacatgaca ggggttcccc atttacggct
    57781 ttcttccatg ccaatcagca tatttctcac gttcgattcc gagctgataa gaaaatcatc
    57841 ttgcaagata taaaatggtc caattctcaa ttttccttca ttgattgctt gttggattgc
    57901 ctgtcttttt tctggcctta cttgaagata gtcatctaaa ataatcgttt gcccatccaa
    57961 atgaaaactg ttgaaggacg gatcaacttc aaatagctct agtagatcgt ctatcaattc
    58021 aatcagtcgc atgtgatgct gttcgtaagc catgtaccat tcacgatccc agtggctatg
    58081 agaaatgata tatactttct ttttcattta cgatacctcc tacttttcta ctctaatatc
    58141 aaagtaatcc atgactaatt cgcaaaacat catatttgcc caagaaaacc attctctcgt
    58201 ataattttca ggattgttca catcgaatcc ttcgtgcata aggtgtgttc ctgcgtcagt
    58261 tgctacaagt aaattcaata tccgctcttt ttcagatttg tctgacgttg tcattccttc
    58321 catagctaat gcaatcggcc aaacgtattt ttctggcgta tgggaggatc caattccttt
    58381 tgcgtacttt ccttcataga aatatggatt ttctttactc aacaatgttc ttcttgtttg
    58441 cagataatct tcctcttgtt cagaacaata tcccaagtaa ggtgcggcga tcagattggg
    58501 cacattactg tcatccatga ctgtggcgtt tcccattcca tctacttcat atgcataaat
    58561 cgtttgccct ttttgattaa ccgttcgtcc gtgattctga atcccctctt ggatctcttt
    58621 cttcaatttc tgtgtttgct gtacaatttc tttatcatcg agtatcgttt gaaaaatttc
    58681 ttccacataa cctaacacga ccaccgcaaa catattggat ggtaccagat atccataacg
    58741 gcaagtatca tcgctaggac ggaaaccaga ccatgtcatt cctgtaaaag ctgtttttgc
    58801 acctcgcccg tcattgatca gcgtatcttc ttttcttgtc gtgtctcgta caaatgtata
    58861 aggagagtgt tcatgatctt gctctgtttt gaacacgtcc aagatttttt tgataccttc
    58921 aataaaagat tgattgaatt gatcagtttt tcctgtattt ttgtaaagaa gataggccaa
    58981 ctgtattggg tagcataaag aatcaatttc atattttctt tcccagatcc aatcgttcat
    59041 ttttgtatga tctgtttggt ggccagcacc gttagcttct tcgttgaagg cattcgcata
    59101 tggatcgata ttgatataaa aaaattgctt tttgactaac ccgctgatca tgtcagctaa
    59161 ttcctcatct tcttttgcaa tcactaaata aggacgaact tgtgcagtag agtctcttaa
    59221 ccacatcgct ggaatgtctc ccgtcaataa aaaggtggtt ccgtcatttt gctttttgac
    59281 tgttgtcaac agagtattgg caaatgctgc tgtaaaattc tttgcccagt ccgcatggat
    59341 atccccacat ttttgtgtga tatcttccat aaatttctgc accgattttg gtatatcttt
    59401 ataagccact aataaaacct tctttctatt tgatatatct ttaaagatta tacttcatac
    59461 agccaattat gtctacgctt tcaaaaaata tctttttatt tactttttca tccattatgg
    59521 tattatttga atatgatata tcattaagag gtgtttatat gaatcaacca ttgtaccaga
    59581 caatatttaa tgatttgcaa acggctattt taactggtga attacctgta gacagccaac
    59641 taccaactga aaaagaactt tcacgtacct ataaagtaag cagaatcact tccaaacgtg
    59701 cattgactga attagagcaa ttaggactta tttatcgcgt tcgcgggaaa ggcagtttcg
    59761 ttaaatccgc ttctccggaa cagattcttg cttctcctac aaaaacacga cggattctgt
    59821 ttcttcttcc atatgtcgct gatttgtctg ttggtgattt tagcaaggga ttgaaccctg
    59881 tgatgcaaga taaacagatt gatgtcatga tgacgacgct tgactttcta caaaataaaa
    59941 cagcaaaaga gctaatcaat gaatttgacg gattgattta ttatgctctt gatacagaac
    60001 aacatcttga cttacttttt gagctttcat tgaaagaatt ccctgtcgtc atattagata
    60061 aaaaaatcta tgagttacct tttcctaccg ttctttccga taatttccaa ggcggtgcgc
    60121 ttgctgcctc ttatttgatc gaacagggac attcaaaaat cgcttatttg tttggtacgc
    60181 agactcatcc acaatccgtt cgacagcgtt accttggtta ccttgaagca ttggacaaag
    60241 ctggtttgac tttccacact tcactggatg acaaaagcgc gacattagat gaattgcttt
    60301 cttatatcac taagtatgaa gtaactgctt ttgtttgtga aaacgatgtt gtcgctatcc
    60361 agaccatgaa tctcttgaga cagcatggtt atcatatccc tgaagatttt tcaattatcg
    60421 gatttgatga tatccaagca gcagctcttg ttgacccgcc actgacaacc atcgcacaag
    60481 attttgaaca gttaggtaag cttgcgggag atgctttggt cacttggtta gagacaaaag
    60541 atcgaccatc cgatagtaaa tttccagttt cattcatcaa aagacaatca acaaaggaga
    60601 aataaaatga atatcggtca aatagatact agacatgcaa cagcgaatca gccaaatttc
    60661 tctaatggga actgccagcc ttatactggc gttccttttg ggatgaatta ttttgcaccg
    60721 caaactacgg atcaaaacgg cagctggtgg tttcatcctg atgaccgcgt ttttcaaggc
    60781 taccgtctta cccatcaacc aagtccttgg atgggtgatt tcagtcatat gctgtttacg
    60841 ccgatcaatg gaaaactaca ggagaacacc cttttccatg ctcaaagttc ttaccgccca
    60901 gaagaaagta tattttgccc gactcatctg tcgatctgtc aattacgtta tggcatctgt
    60961 tctacattga tacctagcat gtatggcgga atcttatcta ttgattacag caaaaatgac
    61021 agcggtttac ttttatcctt tccgggacgc catcagctct ttgttataga tccttacaca
    61081 gtggaaggaa aaatcacaca ttttgctggt tcagaagatc ctgatttttc tttttatttt
    61141 atattgagat ttgaatatcc tttaacaatt gatgagttag ctgtttccaa caaaaatagc
    61201 gactcgatcc cgttgtattt tgaacagaca aaaaaacaaa ctatccgatt tgggacatct
    61261 tttatcagct cggaacaagc agcttataac ctggaactag aacaaaacca aagcaaagag
    61321 acttaccttg ctcacagtca agaacaatgg cagtcctatt ttgatcgtat acagctcaca
    61381 caccatgatc aacaacaatt gcggacgttt tatcataatt tatatcgggt gtttttattt
    61441 ccacaaacat tttatgaaat tgatcctagt gggcaaaaaa tccactatga tacgactagt
    61501 cgatctataa aacctggtgt tctctataca aataatgggt tttgggatac gtataaaacg
    61561 gtttatcctc tttattcttt gattgctgta gaaaaatacg aagaaatgct ggaaggtttt
    61621 ttgaacagct atagagaaag tggctatttg ccaaaatggc tgtctcctga tgaacgtgga
    61681 cttatgcctg ggacattgat cgatgcggtc attgcagatg ctgctgtaaa agatatccga
    61741 cgagacttga tgcctgaatt tctaacggca atggaaaaag gtgctacggt tcaaagcaaa
    61801 catccaaatt atggacgcca aggaacagaa gattacttga catatggcta tgttcctaat
    61861 cactaccacg aatcagtcaa tcacacatta gactatgctt atagcgattt ttgtattagc
    61921 caagttgcca aggtactaga agaagaggaa atgacgaaga aatatcagca gcaatcattg
    61981 aactatcaaa atatctttga ctatgagtca ggatttatgc gttcaaaaga taaagatggc
    62041 aagttcagag aaccattttc agctaatcgt tggggacaag attatgcaga aggaagtgct
    62101 tggcaaagca gctttgctgt ttatcacgat ttttcaggac tgatcggaaa atatggtggc
    62161 agtgagagat ataaagaaaa actcattgag ctgtgcaatc agcccccaac gtttgaccca
    62221 ggaggatatg gttttgagat ccacgaaatg agtgagatgg ccgcgataga atttggacaa
    62281 ttagctattt caaaccagcc aagttttcac tatccttact tgttcagtta tataggacaa
    62341 cctgaaatgg cacagccact gcttaaacag ttgatgactc agaccttcga cgattctcca
    62401 atgggttacc ctggagatga ggacaatgga agtatggccg cttggtatat cttcaatagt
    62461 ctgggatttt atcctgtcac gcctggaacc ggtgaatatg ttatcggtat gccattaatt
    62521 gacaaggctg ttttgctttt gtcaaatgga aaggaattga cgattgaagc aacgcctaat
    62581 cggccgcagc aacaatttat ccataaaatc aaacggatgg acaaagaata tacgaaatta
    62641 tattttaccc atcaagattt gctgaaaggc ggcacgatct cttaccaatt agggatcatc
    62701 cctcacccta agaaatatca agcaaaagat ttgccttttt ccttatcaaa ctataattaa
    62761 caagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaataga
    62821 tcctcatatt tttcgtttgg ttggtcttat taccgcagat gcaagctttt gaacaccgtt
    62881 tattgagagg ttgtgaaatc agcgttctga cctgagtatt agagagacaa atggcaaaat
    62941 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    63001 ttagtggaga ggtcgtgaaa aagctg
//
LOCUS       DACCQP010000007        61634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCQP010000007 DACCQP010000000
VERSION     DACCQP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.007"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IVO87_000519"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IVO87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7448743.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="IVO87_000520"
     CDS             complement(2945..3379)
                     /locus_tag="IVO87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448744.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="IVO87_000521"
     CDS             complement(3396..4601)
                     /locus_tag="IVO87_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7448745.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="IVO87_000522"
     CDS             5295..5681
                     /locus_tag="IVO87_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7448746.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5966
                     /locus_tag="IVO87_000523"
     CDS             5775..5966
                     /locus_tag="IVO87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448747.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6480..6812
                     /locus_tag="IVO87_000524"
     CDS             6480..6812
                     /locus_tag="IVO87_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7448748.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7108..7263
                     /locus_tag="IVO87_000525"
     CDS             7108..7263
                     /locus_tag="IVO87_000525"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7448749.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7539..8879
                     /locus_tag="IVO87_000526"
                     /pseudo
     CDS             7539..8879
                     /locus_tag="IVO87_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9120..10736)
                     /locus_tag="IVO87_000527"
     CDS             complement(9120..10736)
                     /locus_tag="IVO87_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7448750.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEVCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10854..11072
                     /locus_tag="IVO87_000528"
     CDS             10854..11072
                     /locus_tag="IVO87_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448751.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11267..11728
                     /locus_tag="IVO87_000529"
     CDS             11267..11728
                     /locus_tag="IVO87_000529"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7448752.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11921..12160)
                     /locus_tag="IVO87_000530"
     CDS             complement(11921..12160)
                     /locus_tag="IVO87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448753.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12184..13707)
                     /locus_tag="IVO87_000531"
     CDS             complement(12184..13707)
                     /locus_tag="IVO87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7448754.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13725..13847)
                     /locus_tag="IVO87_000532"
     CDS             complement(13725..13847)
                     /locus_tag="IVO87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7448755.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13877..14860)
                     /locus_tag="IVO87_000533"
     CDS             complement(13877..14860)
                     /locus_tag="IVO87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7448756.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IVO87_000534"
     CDS             complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IVO87_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7448757.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15054..15431)
                     /locus_tag="IVO87_000535"
     CDS             complement(15054..15431)
                     /locus_tag="IVO87_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448758.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IVO87_000536"
     CDS             complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IVO87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7448759.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IVO87_000537"
     CDS             complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IVO87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7448760.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16449..18221)
                     /locus_tag="IVO87_000538"
     CDS             complement(16449..18221)
                     /locus_tag="IVO87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448761.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18175..19929)
                     /locus_tag="IVO87_000539"
     CDS             complement(18175..19929)
                     /locus_tag="IVO87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448762.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20039..20608)
                     /locus_tag="IVO87_000540"
     CDS             complement(20039..20608)
                     /locus_tag="IVO87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7448763.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20626..22050)
                     /locus_tag="IVO87_000541"
     CDS             complement(20626..22050)
                     /locus_tag="IVO87_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7448764.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22052..22720)
                     /locus_tag="IVO87_000542"
     CDS             complement(22052..22720)
                     /locus_tag="IVO87_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7448765.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLTEKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22850..23434)
                     /locus_tag="IVO87_000543"
     CDS             complement(22850..23434)
                     /locus_tag="IVO87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7448766.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23765..23998)
                     /locus_tag="IVO87_000544"
     CDS             complement(23765..23998)
                     /locus_tag="IVO87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7448767.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24013..24963)
                     /locus_tag="IVO87_000545"
     CDS             complement(24013..24963)
                     /locus_tag="IVO87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7448768.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24960..25802)
                     /locus_tag="IVO87_000546"
     CDS             complement(24960..25802)
                     /locus_tag="IVO87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7448769.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25804..26523)
                     /locus_tag="IVO87_000547"
     CDS             complement(25804..26523)
                     /locus_tag="IVO87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7448770.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27215..27373
                     /locus_tag="IVO87_000548"
     CDS             27215..27373
                     /locus_tag="IVO87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448771.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27445..28671
                     /locus_tag="IVO87_000549"
     CDS             27445..28671
                     /locus_tag="IVO87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7448772.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IVO87_000550"
     CDS             complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IVO87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7448773.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IVO87_000551"
     CDS             complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IVO87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7448774.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30129..30596)
                     /locus_tag="IVO87_000552"
     CDS             complement(30129..30596)
                     /locus_tag="IVO87_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7448775.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30716..32254)
                     /locus_tag="IVO87_000553"
     CDS             complement(30716..32254)
                     /locus_tag="IVO87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7448776.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32334..33020
                     /locus_tag="IVO87_000554"
     CDS             32334..33020
                     /locus_tag="IVO87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7448777.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33221..33748)
                     /gene="msrA"
                     /locus_tag="IVO87_000555"
     CDS             complement(33221..33748)
                     /gene="msrA"
                     /locus_tag="IVO87_000555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7448778.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33832..34509)
                     /gene="pgmB"
                     /locus_tag="IVO87_000556"
     CDS             complement(33832..34509)
                     /gene="pgmB"
                     /locus_tag="IVO87_000556"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7448779.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34506..35636)
                     /locus_tag="IVO87_000557"
     CDS             complement(34506..35636)
                     /locus_tag="IVO87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7448780.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35636..37819)
                     /locus_tag="IVO87_000558"
     CDS             complement(35636..37819)
                     /locus_tag="IVO87_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7448781.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37816..38841)
                     /locus_tag="IVO87_000559"
     CDS             complement(37816..38841)
                     /locus_tag="IVO87_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7448782.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38886..39713)
                     /locus_tag="IVO87_000560"
     CDS             complement(38886..39713)
                     /locus_tag="IVO87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7448783.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39710..40777)
                     /locus_tag="IVO87_000561"
     CDS             complement(39710..40777)
                     /locus_tag="IVO87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7448784.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40803..41639)
                     /locus_tag="IVO87_000562"
     CDS             complement(40803..41639)
                     /locus_tag="IVO87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7448785.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41636..42505)
                     /locus_tag="IVO87_000563"
     CDS             complement(41636..42505)
                     /locus_tag="IVO87_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448786.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42575..43858)
                     /locus_tag="IVO87_000564"
     CDS             complement(42575..43858)
                     /locus_tag="IVO87_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7448787.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43872..45557)
                     /locus_tag="IVO87_000565"
     CDS             complement(43872..45557)
                     /locus_tag="IVO87_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7448788.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45832..46857
                     /locus_tag="IVO87_000566"
     CDS             45832..46857
                     /locus_tag="IVO87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7448789.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47452..51105)
                     /gene="rpoC"
                     /locus_tag="IVO87_000567"
     CDS             complement(47452..51105)
                     /gene="rpoC"
                     /locus_tag="IVO87_000567"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7448790.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51184..54801)
                     /gene="rpoB"
                     /locus_tag="IVO87_000568"
     CDS             complement(51184..54801)
                     /gene="rpoB"
                     /locus_tag="IVO87_000568"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7448791.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55529..56497
                     /locus_tag="IVO87_000569"
     CDS             55529..56497
                     /locus_tag="IVO87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7448792.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56515..57420
                     /locus_tag="IVO87_000570"
     CDS             56515..57420
                     /locus_tag="IVO87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448793.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57417..58223
                     /locus_tag="IVO87_000571"
     CDS             57417..58223
                     /locus_tag="IVO87_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7448794.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58380..59348
                     /locus_tag="IVO87_000572"
     CDS             58380..59348
                     /locus_tag="IVO87_000572"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7448795.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59665..60189)
                     /locus_tag="IVO87_000573"
     CDS             complement(59665..60189)
                     /locus_tag="IVO87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7448796.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60476..>61634
                     /gene="scm"
                     /locus_tag="IVO87_000574"
     CDS             60476..>61634
                     /gene="scm"
                     /locus_tag="IVO87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7448797.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTT"
BASE COUNT        18704 a        12376 c        10320 g        20234 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     5881 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     5941 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     6001 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     6061 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     6121 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     6181 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     6241 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     6301 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     6361 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     6421 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     6481 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     6541 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     6601 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     6661 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     6721 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     6781 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     6841 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     6901 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     6961 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     7021 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     7081 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     7141 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     7201 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     7261 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     7321 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     7381 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     7441 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     7501 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     7561 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     7621 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     7681 aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc tggaaaaaag
     7741 tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt aacaaacagc
     7801 gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt agtgatcgtc
     7861 gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc gaacctcttt
     7921 ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc
     7981 gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg gtgtgtttcg
     8041 atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg atggacggca
     8101 agacccatca actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt
     8161 tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca gatttctata
     8221 caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct
     8281 ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga
     8341 cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa ctacttcaaa
     8401 agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt cgagatcatc
     8461 tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta aagcgaggct
     8521 acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct gaattcgtcg
     8581 cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg ttcacgacct
     8641 tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc aacggcccta
     8701 tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc tatagaaact
     8761 ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag aaaactaaga
     8821 atactaccca actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc
     8881 cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc
     8941 aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg
     9001 tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc
     9061 ctgctattat taataataat atctttttct tattaaaaac ctcctcatct atttccagat
     9121 tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa cttatggggt
     9181 aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca
     9241 ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt
     9301 cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc
     9361 atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt tgtttcattc
     9421 tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa aatgccaata
     9481 attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg
     9541 ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta
     9601 agagaatcag ttaaacaaac ctcaagttgt tccttaatac attgttgatc catcttcata
     9661 ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat
     9721 tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc tacccaataa
     9781 tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga aaaatcacta
     9841 tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa atttttatcc
     9901 gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag
     9961 ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa ctctttgaac
    10021 cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag aattttcgat
    10081 aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa cttagcaatt
    10141 agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt taaaagtaat
    10201 atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc ttcttcaact
    10261 acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa
    10321 cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt caaatcaatg
    10381 tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt gctgattcct
    10441 gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc tagttcaaca
    10501 atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac aagtttttct
    10561 ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac ttcccctatg
    10621 tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg atttagaagt
    10681 atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt tttcataggg
    10741 aacctctttc tataccttga atttacactt taagtgcaac acataaaaat gagtcataat
    10801 cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc
    10861 agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac gaagaaggat
    10921 tttctattag tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac
    10981 tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt atgcttatcg
    11041 aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg
    11101 tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga
    11161 acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca atgatctttt
    11221 cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg
    11281 aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc ttactcattc
    11341 gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga
    11401 tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca gaaaatcatg
    11461 cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat
    11521 gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg
    11581 aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc ccttttattt
    11641 tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga
    11701 gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg
    11761 attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc
    11821 ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa agcagatcaa
    11881 atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat
    11941 gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat
    12001 cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag
    12061 ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga taagcatcaa
    12121 tattcttgga atccatagac aataatactt ttcttaccat gtatttcctc caaaaattga
    12181 attttaatga gccatcattt cttgagcaat ttcatgttta gacaatgaag ctggatagaa
    12241 agtaggccaa ttatgcaatt cattatagag tttttcttga ctttctccac caaagaaaat
    12301 atggaaatgc tctgccttac ttggtgcaat tccatgatca ctaaattgaa tatatttaaa
    12361 acgaccagca cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc
    12421 ataattcaaa attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac
    12481 tacaaaatca atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt
    12541 agtataataa tctttgtact cggcagctgt catgtcttta ttgatttttg ctttgtaatc
    12601 gaaaacttca tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt
    12661 ataatcactc aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt
    12721 cttttcatcc ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt
    12781 ttctaacgct tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg
    12841 ttcttttgta agactttcaa gaggattcag tacttccaat tttatcccag cttcgttggc
    12901 caaagttttt gcaattttat cattagcatt cttttcaaag taaatataat tgatcttatt
    12961 ttttttaaca tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact
    13021 tagtccagca ataggaactt gtttcaatcc ataatccaaa gctaaatatc caaaggctgc
    13081 gtgttgcgtc acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc
    13141 caaccgtttc agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt
    13201 cggatacaat ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg
    13261 tgaaacccac gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc
    13321 ttcttcttca ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc
    13381 agctttcttc caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata
    13441 tacaaaaaca tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg
    13501 aggttctgat cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt
    13561 ggtgaaatca tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt
    13621 cgatactttt gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc
    13681 aataagtcct acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc
    13741 attttatttc gtttatgttc tttgataatt tttaatgccc tttttctagt tttaatattc
    13801 gggcttttaa gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc
    13861 caatcactac ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc
    13921 tttcttcatc caacattttt ccgattagaa taatagtggt ttggtctttt tgtcgagtgg
    13981 tgtattgaaa attaacctgt tcgtttacct cttgcatggc aactataaaa tcctgctctt
    14041 gtaaagcaat caatccttta aaacgataca ggctcccttg gttggttaaa atcagccagt
    14101 caatccattg gagtaaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt
    14161 taaactcatg tacttgatgg tgttgatgat agtatttgcc attatagtga ttttctgatg
    14221 aatcactttc aaaaactaag ggatgattaa attttttcaa attataaaaa tccttttttt
    14281 gaatcgtttc actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt
    14341 ctctctgtaa tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc
    14401 gatctgccat gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt
    14461 cactatcaat aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg
    14521 ttaaaagtgt ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt
    14581 gtgaaattgg ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata
    14641 aatcactgcg aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc
    14701 tatgaactaa taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa
    14761 tttcttccct ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag
    14821 cccctaaaaa cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact
    14881 tagctatttc acttctttaa aaattgcccg tctgcgcaat tttggcgaat acttttttaa
    14941 ttctaaccgt tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt
    15001 ttcgacacat tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga
    15061 tcttcaagat atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat
    15121 tgtgaaacta aattttcttt catatcaaca ggtatctccc aataaccatc gatacattga
    15181 aataactcct cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat
    15241 aagccttggt cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt
    15301 aaacccgttt cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct
    15361 agcgattgct gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa
    15421 ataatttcca ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt
    15481 aattactttc tttccattat agtacccttt taaagataca tgatggctac gtctataatc
    15541 accattcgct tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa
    15601 ccgtttcttc gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac
    15661 caacttgctt tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata
    15721 cgggacatac cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc
    15781 ctcagacgat tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg
    15841 gcttttaatt cctgacgttt atctgcatac ttttcaatca tcgccatttg tttctttgct
    15901 ttagcaattt tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca
    15961 cctcaaatcg taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt
    16021 caacaataaa taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt
    16081 atcggtacat agagataatc aaatcattta aaagaatatg cagtttaggc tctgaaaaat
    16141 aagaattcac ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct
    16201 acttaaaacg gcaatatgaa agaaactata actttttatt caatctttta tgtttaactg
    16261 gtttatcgcc aattttttaa aactttgatt attccttata ttataagaat agacattaag
    16321 tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa
    16381 attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc
    16441 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
    16501 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
    16561 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
    16621 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
    16681 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
    16741 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
    16801 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
    16861 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
    16921 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
    16981 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
    17041 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
    17101 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
    17161 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
    17221 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
    17281 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
    17341 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
    17401 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
    17461 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
    17521 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
    17581 aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta
    17641 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
    17701 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
    17761 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
    17821 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
    17881 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
    17941 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
    18001 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
    18061 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
    18121 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
    18181 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
    18241 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
    18301 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
    18361 aactgttttt tcttttatca gatgttgaaa agccttttgt agttcatact cattatctgg
    18421 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
    18481 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
    18541 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
    18601 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
    18661 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
    18721 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
    18781 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
    18841 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
    18901 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
    18961 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
    19021 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
    19081 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
    19141 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
    19201 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
    19261 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
    19321 tccgcggata tattctaccg tttcagagct taacacatct aacgattctt gatatttttg
    19381 aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc
    19441 gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg
    19501 tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc
    19561 cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa
    19621 acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc
    19681 aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc
    19741 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
    19801 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
    19861 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
    19921 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
    19981 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
    20041 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
    20101 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
    20161 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
    20221 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
    20281 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
    20341 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
    20401 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
    20461 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
    20521 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
    20581 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
    20641 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
    20701 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
    20761 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
    20821 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
    20881 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
    20941 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
    21001 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
    21061 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
    21121 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
    21181 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
    21241 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
    21301 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
    21361 taacaactga atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat
    21421 taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt
    21481 taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg
    21541 ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag aaattttctg
    21601 ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa
    21661 ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat
    21721 cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact
    21781 tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc
    21841 aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa
    21901 ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt
    21961 ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga
    22021 aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag
    22081 taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc
    22141 ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca
    22201 tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa
    22261 aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat
    22321 aagtcggaaa gaatctgatc actactgtaa tacttaacat tattttttcc gttaaatgta
    22381 ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga
    22441 tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa
    22501 aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca
    22561 agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa
    22621 tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa
    22681 tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata
    22741 agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc
    22801 ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat taactcagta
    22861 attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt
    22921 cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga
    22981 agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact
    23041 tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt
    23101 gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa
    23161 tataattaat aaatggatct aattcattga agttctctct ccaacttttc tctatatcta
    23221 tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat
    23281 taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa
    23341 aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt tgctgaaatc
    23401 cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt ataatataat
    23461 ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta tcttacacag
    23521 gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag ctaaactaat
    23581 gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata gactcatcta
    23641 aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt
    23701 aggataagtc ctatcataat cactccaata gccattttct tatcctccct aaaaataaag
    23761 tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt
    23821 gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg
    23881 ctggactaac acttagaact tttgctaaat cattattttt taatttcttt tttctttttg
    23941 ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac
    24001 ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat
    24061 cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga
    24121 atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat ccacactgct
    24181 ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg
    24241 atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata
    24301 cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg
    24361 aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt
    24421 atctagattt tctttataga attttttatt atcaggatct ttttccgcta actgtttttc
    24481 aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag gatcttcctt
    24541 ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata
    24601 gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag catttcctcc
    24661 agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc
    24721 ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc
    24781 aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag taactactac
    24841 ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg aacatgcccc
    24901 gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc attttctcat
    24961 cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt
    25021 acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa
    25081 ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat aaatatcata
    25141 gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa cattgcaaca
    25201 attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaaccatc
    25261 aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa tggatcaaaa
    25321 gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa aaccgaaacg
    25381 ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa taaaatatga
    25441 aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc taaaaaagaa
    25501 ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta
    25561 ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa
    25621 atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac
    25681 attcctctta aaataataaa acaacctact gttccagaaa caatccctat aactacagat
    25741 gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc
    25801 atattattcc ccctctccaa taaatatagt atctccatag gcttttttta aattttcttc
    25861 attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa gaataatatt
    25921 atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg tttttccctg
    25981 atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt ctatccctac
    26041 aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg ctaaaagtac
    26101 acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt ttcccatatc
    26161 gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc tttgaaaaac
    26221 tttcattccg gcatatgttc ccatagatac acattctttt acttttattg gaaaagtaaa
    26281 atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat tgatatcttc
    26341 attattaatg gagacctctc cctgatgagg gataatattt aacattgctt taagcaatgt
    26401 ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt gtatttttaa
    26461 agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa
    26521 cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata caaaaattat
    26581 attttagtca accctaaatt ttatagttta gatttttttc tgttgctgaa gattagaatg
    26641 acaaaactat ttgaaactac gcgtatagta agtagctttc aaaatatata aaaaaggagc
    26701 gttctcaaat gagaacatgc tcctaaaact actttattgg cgtatggaaa aattatactt
    26761 gaagtggata gcgggttgaa taagttgata agcatcaaaa catttataaa aattattcaa
    26821 gactatgtct gttccatata caaatatgaa aatcagtaaa ctccatttaa cttttttcgt
    26881 agtgtgtctt tttatacatg tttatcgtta ttaatagtga ataggaaaaa aactattttt
    26941 gatcattgac aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact
    27001 caagtacgcc atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa
    27061 acaaaatgcc atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg
    27121 gggagaggtg aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat
    27181 tttaggagat gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt
    27241 tctaggaatc gataataata ctctagataa gtggattcgt atatataatt tcccagtaat
    27301 aagagttggt ggtgtagttc gctttgacgt agaagatatt aaagacttta tgaatacatt
    27361 taaaataact tgaaattttc ttctcgccgc atatactaaa attagtgaaa tatactgcct
    27421 tacttttttt aaggagtgag gattttgcct acaaagcttt caaatgggaa atataaaact
    27481 aatctgagat atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa
    27541 aaaacatttc caaataggca attggcgatt aaagccgaaa atgatatgaa gaagaaaata
    27601 gaaaaagttt tgagagagga gaatgcaaac tcattagaat taaaaggaaa aataacgttt
    27661 aaaaaatttt acgaatctaa atggcttcct agatatgaat taggtcaaac aattagatca
    27721 aacaggcctc ctagtgatat aaccattagt aatacaaaag atatatttcg gttgcatatt
    27781 ttacctatgt ttggtgaata cgcaatgaat tatctaaata tcaatactga gataatatca
    27841 gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa aggatatgtt
    27901 cgttccatgt tcgatattgc agaaatacta aattatatcg aatttaatcg aactactaaa
    27961 attattcaaa gtatcactgc acctaaaaaa aatgctttag aagaaaaaag aatacaagaa
    28021 ggtaagcaag cactatcatc aaaagaactt actaattgga tagaagcagt aaatgatgat
    28081 tttaataatc atttacttac ttttcatgat tacacacttt ttatgattac cctgtatctt
    28141 ggtgatagaa aaagtgagac ttacgctttg caatggaaat atattgattt tgaaaaacaa
    28201 acggtccgtc ttaaacatac tttagataaa tatcaaagaa aaaagtttac aaaaggtcga
    28261 aaagataccg tcattcaagt accagaagtt gtaatgacat tgctaagtga atggaaatcg
    28321 gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt
    28381 acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat
    28441 cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc
    28501 cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat
    28561 gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt
    28621 gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc
    28681 ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac
    28741 gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag
    28801 acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag
    28861 agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc
    28921 tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt
    28981 tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta
    29041 ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg
    29101 agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc
    29161 ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg
    29221 ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg
    29281 aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc
    29341 agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt
    29401 cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt
    29461 atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt
    29521 tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc
    29581 gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt
    29641 ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat
    29701 tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac
    29761 tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa
    29821 agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc
    29881 caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg
    29941 tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc
    30001 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg
    30061 tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt caagttcaac
    30121 aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat catccagata
    30181 cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc gtcgccttct
    30241 tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata gcctgcaaca
    30301 agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc agggattttt
    30361 gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt
    30421 tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat cattgggtgc
    30481 aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac aacagacatt
    30541 tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa tttcatcttt
    30601 cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg aaaagtacaa
    30661 caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa gccaatcatt
    30721 tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat
    30781 gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga tcagtttctt
    30841 gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata ctttggatct
    30901 ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct gtgacagtag
    30961 ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc ggagattgag
    31021 caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact ccttttaaaa
    31081 tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct ttttctgtat
    31141 gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct
    31201 caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag
    31261 ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct
    31321 gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca
    31381 ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag
    31441 gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat
    31501 caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg
    31561 atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat
    31621 aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt
    31681 cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt
    31741 cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag
    31801 cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg
    31861 tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt
    31921 tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg
    31981 gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag gagacaaatt
    32041 gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt
    32101 gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc
    32161 gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg
    32221 tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt
    32281 acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac
    32341 tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc
    32401 cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt
    32461 cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt
    32521 gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg
    32581 cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg
    32641 ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct
    32701 gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg
    32761 tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag
    32821 ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc
    32881 agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc
    32941 ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg
    33001 tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat
    33061 atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc
    33121 cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac
    33181 ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat
    33241 gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt
    33301 ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga
    33361 tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct
    33421 ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc
    33481 gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca
    33541 actgatcata agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg
    33601 ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg
    33661 atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc
    33721 cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg
    33781 atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg
    33841 tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg
    33901 aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt
    33961 accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg
    34021 cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga
    34081 tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat
    34141 gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta
    34201 ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg
    34261 aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg
    34321 ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag
    34381 ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc
    34441 ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt
    34501 gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta
    34561 gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca
    34621 ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca
    34681 acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact
    34741 ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct
    34801 gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact
    34861 attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc
    34921 gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc
    34981 actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct
    35041 tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca
    35101 ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct
    35161 actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg
    35221 ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac
    35281 aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt
    35341 agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct
    35401 gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc
    35461 ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt
    35521 agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct
    35581 gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt
    35641 ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa
    35701 taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata
    35761 gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc
    35821 ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg
    35881 ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg
    35941 gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga
    36001 tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt
    36061 tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt
    36121 ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg
    36181 cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa
    36241 ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca
    36301 aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat
    36361 atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga
    36421 tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt
    36481 ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt ccctttgttt
    36541 ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg
    36601 cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat
    36661 tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa
    36721 gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac
    36781 ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa
    36841 aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc
    36901 ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt
    36961 ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa
    37021 aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg
    37081 cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga
    37141 tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc
    37201 ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa
    37261 ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt
    37321 ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca
    37381 catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga
    37441 gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt
    37501 caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa
    37561 gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga
    37621 catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag
    37681 ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa
    37741 aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact
    37801 gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt
    37861 ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc
    37921 accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt
    37981 ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag
    38041 attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa
    38101 agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat
    38161 tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt
    38221 cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat
    38281 atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag
    38341 ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc
    38401 tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga
    38461 aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat
    38521 ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc
    38581 ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat
    38641 atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt
    38701 acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa
    38761 gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc
    38821 tatgattgct actttcttca tttatttctc cctcctttgc ttatttactt attgttattc
    38881 tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag
    38941 tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat
    39001 accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt
    39061 gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct
    39121 ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca
    39181 gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac
    39241 aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt
    39301 tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct
    39361 ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg
    39421 ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt
    39481 acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa
    39541 gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct
    39601 attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat
    39661 ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc
    39721 ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca
    39781 tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca
    39841 agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg
    39901 ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat
    39961 gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa
    40021 tgatcccgcc atagccgatc cctcttaaag aagcttgtaa tgcggcagca ctgccactcg
    40081 tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat
    40141 ctgctttcat aggatctagc gtgtaatcgg caccgtattt ttctgctaat tctctacgct
    40201 gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg
    40261 caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg
    40321 cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt
    40381 gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt
    40441 cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc
    40501 ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac
    40561 cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat
    40621 cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga
    40681 aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc
    40741 cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct
    40801 ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca
    40861 tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg
    40921 tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc
    40981 gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag
    41041 acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg
    41101 ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt
    41161 tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta
    41221 ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata
    41281 ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt
    41341 agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc
    41401 aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa
    41461 atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt
    41521 gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca
    41581 gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt
    41641 tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa
    41701 tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa
    41761 cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc
    41821 agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga
    41881 tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt
    41941 cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag
    42001 caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt
    42061 tgaaaagctg caacctatct actaacagat cattgacgat tccataacta ttattgaaca
    42121 tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg
    42181 aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta
    42241 aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat
    42301 aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg
    42361 agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca
    42421 gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt
    42481 cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc
    42541 tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag
    42601 ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca
    42661 cacctgagct tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga
    42721 cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga
    42781 cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac
    42841 caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg
    42901 cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg
    42961 tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc
    43021 ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat
    43081 tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata
    43141 agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt
    43201 catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca
    43261 ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat
    43321 gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata
    43381 atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc
    43441 tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag
    43501 cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt
    43561 tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa
    43621 cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat
    43681 tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag
    43741 aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac
    43801 aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt
    43861 ttttccacct cttattttat tattttcagc cacatatatt catacggctg gaatatcaac
    43921 tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt
    43981 ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc
    44041 ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta
    44101 ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat
    44161 actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt
    44221 tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt
    44281 cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga
    44341 gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct
    44401 agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt
    44461 actcgggtac cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga
    44521 atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt
    44581 gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc
    44641 gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca
    44701 ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc
    44761 aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc
    44821 gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc
    44881 aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat
    44941 aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg
    45001 tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta
    45061 gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca
    45121 ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag
    45181 tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg
    45241 tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat
    45301 ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat
    45361 gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata
    45421 tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact
    45481 ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt
    45541 aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat
    45601 catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa
    45661 aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct
    45721 ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat
    45781 tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct
    45841 actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta
    45901 aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa
    45961 ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt
    46021 ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt
    46081 attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca
    46141 aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga
    46201 ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatac
    46261 aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg
    46321 gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac
    46381 cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat
    46441 ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac
    46501 ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa
    46561 agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca
    46621 gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta
    46681 ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga
    46741 atcaaagcca cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga
    46801 atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac
    46861 agaattataa aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc
    46921 actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt
    46981 ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa
    47041 aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta
    47101 cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca
    47161 gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt
    47221 aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt
    47281 acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa
    47341 ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa
    47401 ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt
    47461 ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac
    47521 attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt
    47581 accagctggg atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt
    47641 cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga
    47701 taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt
    47761 tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc
    47821 taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag
    47881 catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta
    47941 aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa
    48001 caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc
    48061 ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc
    48121 tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc
    48181 tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac
    48241 acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat
    48301 cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc
    48361 gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg
    48421 ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat
    48481 ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc
    48541 aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat
    48601 gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc
    48661 ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc
    48721 ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat
    48781 ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc
    48841 attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc
    48901 acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag
    48961 tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc
    49021 gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc
    49081 attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat
    49141 accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt
    49201 ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt
    49261 gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac
    49321 aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg
    49381 gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg
    49441 ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg
    49501 tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa
    49561 gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac
    49621 catatcttgg gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc
    49681 agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc
    49741 catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat
    49801 tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg
    49861 tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc
    49921 gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg
    49981 aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc
    50041 acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac
    50101 acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag
    50161 agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat
    50221 caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc
    50281 attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc
    50341 attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg
    50401 tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa
    50461 tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt
    50521 caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt
    50581 aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg
    50641 atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac
    50701 tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag
    50761 acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc
    50821 aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc
    50881 acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt
    50941 tgtaggacca aaaatccgct cacagaacaa cccttcgcgt tcaggtttta aggtacgata
    51001 gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga
    51061 agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca
    51121 tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc
    51181 ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc
    51241 ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt
    51301 tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat
    51361 ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac
    51421 caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc
    51481 ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa
    51541 agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc
    51601 accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga
    51661 acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac
    51721 agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc
    51781 gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa
    51841 tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa
    51901 tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg
    51961 tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt
    52021 atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata
    52081 aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat
    52141 tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac
    52201 ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt
    52261 tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat
    52321 acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc
    52381 gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat
    52441 atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc
    52501 atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc
    52561 gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa
    52621 atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac
    52681 atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta
    52741 ttcaacgaca ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta
    52801 ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg
    52861 catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc
    52921 tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga
    52981 tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc
    53041 ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa
    53101 gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc
    53161 gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt
    53221 tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg
    53281 aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg
    53341 tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga
    53401 accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt
    53461 caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag
    53521 cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa
    53581 gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga
    53641 agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat
    53701 cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat
    53761 tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc
    53821 aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat
    53881 gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt
    53941 tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt
    54001 tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc
    54061 tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt
    54121 tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa
    54181 gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg
    54241 tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc
    54301 tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc
    54361 atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt
    54421 gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc
    54481 tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta
    54541 gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc
    54601 tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat
    54661 ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt
    54721 tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta
    54781 ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc
    54841 cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg
    54901 cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag
    54961 cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt
    55021 cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac
    55081 ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt
    55141 caattatctg acaattctta attgacagac ctttttatct cggttatact ttagaaaatt
    55201 cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga
    55261 ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt
    55321 aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta
    55381 gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact
    55441 ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt
    55501 gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc
    55561 tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga
    55621 tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta
    55681 taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat
    55741 cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt
    55801 caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa
    55861 tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt
    55921 agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat
    55981 cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat
    56041 gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa
    56101 aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt
    56161 gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc
    56221 aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg
    56281 cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt
    56341 gggtaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact
    56401 tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt
    56461 aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca
    56521 aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg
    56581 attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc
    56641 tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca
    56701 gcttgtcttc taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac
    56761 acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct
    56821 gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt
    56881 cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt
    56941 tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct
    57001 attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat
    57061 atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttgctt
    57121 gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt
    57181 gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg
    57241 actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat
    57301 gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc
    57361 ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga
    57421 atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa
    57481 atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg
    57541 gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg
    57601 atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt
    57661 cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg
    57721 tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg
    57781 tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg
    57841 aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt
    57901 tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc
    57961 ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga
    58021 atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac
    58081 tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc
    58141 tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg
    58201 atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc
    58261 tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg
    58321 aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca
    58381 tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag
    58441 aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat
    58501 tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg
    58561 acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag
    58621 tacaaacgat gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag
    58681 gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg
    58741 gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag
    58801 acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag
    58861 ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg
    58921 atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga
    58981 tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag
    59041 ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga
    59101 atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag
    59161 cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa
    59221 aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc
    59281 aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta
    59341 cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg
    59401 aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa
    59461 aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa
    59521 aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa
    59581 tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc
    59641 gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat
    59701 cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt
    59761 gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct
    59821 taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg
    59881 attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc
    59941 tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc
    60001 taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga
    60061 tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc
    60121 ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa
    60181 ataatccatt tattttcctc ctttttattt atcctactta ctttttccta attctaggat
    60241 acctacttat atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa
    60301 tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa
    60361 tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag
    60421 actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa
    60481 aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt
    60541 ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac
    60601 tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac
    60661 aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa
    60721 ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac
    60781 tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt
    60841 cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag
    60901 gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga
    60961 tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc
    61021 agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac
    61081 tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa
    61141 tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag
    61201 ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac
    61261 agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac
    61321 aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca
    61381 aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg
    61441 ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag
    61501 tacagataca tctaccacaa gtgatagctc agattcatca acttcaagcg acagtacaga
    61561 tactacctct accacaagtg atagcacgga tacatcagct tcaagcgaca gtacagatac
    61621 tacctctacc acaa
//
LOCUS       DACCQP010000008        61520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCQP010000008 DACCQP010000000
VERSION     DACCQP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.008"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            84..971
                     /locus_tag="IVO87_000575"
     CDS             84..971
                     /locus_tag="IVO87_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7448798.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="IVO87_000576"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="IVO87_000576"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7448799.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="IVO87_000577"
     CDS             2273..2719
                     /locus_tag="IVO87_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7448800.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="IVO87_000578"
     CDS             2992..4344
                     /locus_tag="IVO87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7448801.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="IVO87_000579"
     CDS             4515..4802
                     /locus_tag="IVO87_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448802.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5028..5471
                     /locus_tag="IVO87_000580"
     CDS             5028..5471
                     /locus_tag="IVO87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7448803.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5477..6661)
                     /locus_tag="IVO87_000581"
     CDS             complement(5477..6661)
                     /locus_tag="IVO87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HAP7448804.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFVGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6929..7627
                     /locus_tag="IVO87_000582"
     CDS             6929..7627
                     /locus_tag="IVO87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7448805.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7634..8311
                     /gene="nth"
                     /locus_tag="IVO87_000583"
     CDS             7634..8311
                     /gene="nth"
                     /locus_tag="IVO87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7448806.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8437..8970)
                     /locus_tag="IVO87_000584"
     CDS             complement(8437..8970)
                     /locus_tag="IVO87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448807.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9267..11648)
                     /locus_tag="IVO87_000585"
     CDS             complement(9267..11648)
                     /locus_tag="IVO87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7448808.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLTRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11638..12285)
                     /gene="recU"
                     /locus_tag="IVO87_000586"
     CDS             complement(11638..12285)
                     /gene="recU"
                     /locus_tag="IVO87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7448809.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12349..12888
                     /locus_tag="IVO87_000587"
     CDS             12349..12888
                     /locus_tag="IVO87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7448810.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12978..13397
                     /gene="gpsB"
                     /locus_tag="IVO87_000588"
     CDS             12978..13397
                     /gene="gpsB"
                     /locus_tag="IVO87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7448811.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13419..13786
                     /gene="rnpB"
                     /locus_tag="IVO87_000589"
     ncRNA           13419..13786
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVO87_000589"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13995..15161
                     /locus_tag="IVO87_000590"
     CDS             13995..15161
                     /locus_tag="IVO87_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7448812.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15207..16703
                     /locus_tag="IVO87_000591"
     CDS             15207..16703
                     /locus_tag="IVO87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7448813.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16784..17467
                     /locus_tag="IVO87_000592"
     CDS             16784..17467
                     /locus_tag="IVO87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7448814.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18112..19302
                     /locus_tag="IVO87_000593"
     CDS             18112..19302
                     /locus_tag="IVO87_000593"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7448815.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19404..20357
                     /locus_tag="IVO87_000594"
     CDS             19404..20357
                     /locus_tag="IVO87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7448816.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20426..21775
                     /locus_tag="IVO87_000595"
     CDS             20426..21775
                     /locus_tag="IVO87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7448817.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21958..22281)
                     /locus_tag="IVO87_000596"
     CDS             complement(21958..22281)
                     /locus_tag="IVO87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448818.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22410..23573
                     /locus_tag="IVO87_000597"
     CDS             22410..23573
                     /locus_tag="IVO87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448819.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23643..24992
                     /locus_tag="IVO87_000598"
     CDS             23643..24992
                     /locus_tag="IVO87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7448820.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25037..25468)
                     /locus_tag="IVO87_000599"
     CDS             complement(25037..25468)
                     /locus_tag="IVO87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7448821.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25856..28498
                     /gene="alaS"
                     /locus_tag="IVO87_000600"
     CDS             25856..28498
                     /gene="alaS"
                     /locus_tag="IVO87_000600"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7448822.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28698..29294
                     /locus_tag="IVO87_000601"
     CDS             28698..29294
                     /locus_tag="IVO87_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7448823.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29416..30564
                     /locus_tag="IVO87_000602"
     CDS             29416..30564
                     /locus_tag="IVO87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7448824.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30602..31015)
                     /locus_tag="IVO87_000603"
     CDS             complement(30602..31015)
                     /locus_tag="IVO87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7448825.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31094..31525
                     /locus_tag="IVO87_000604"
     CDS             31094..31525
                     /locus_tag="IVO87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7448826.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31624..31857)
                     /locus_tag="IVO87_000605"
     CDS             complement(31624..31857)
                     /locus_tag="IVO87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7448827.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32040..33710
                     /locus_tag="IVO87_000606"
     CDS             32040..33710
                     /locus_tag="IVO87_000606"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7448828.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33857..34243
                     /locus_tag="IVO87_000607"
     CDS             33857..34243
                     /locus_tag="IVO87_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448829.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34230..34928
                     /locus_tag="IVO87_000608"
     CDS             34230..34928
                     /locus_tag="IVO87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7448830.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34933..35397
                     /locus_tag="IVO87_000609"
     CDS             34933..35397
                     /locus_tag="IVO87_000609"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7448831.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35394..36305
                     /locus_tag="IVO87_000610"
     CDS             35394..36305
                     /locus_tag="IVO87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7448832.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36540..37079
                     /gene="pyrR"
                     /locus_tag="IVO87_000611"
     CDS             36540..37079
                     /gene="pyrR"
                     /locus_tag="IVO87_000611"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7448833.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37093..38376
                     /locus_tag="IVO87_000612"
     CDS             37093..38376
                     /locus_tag="IVO87_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7448834.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38389..39315
                     /locus_tag="IVO87_000613"
     CDS             38389..39315
                     /locus_tag="IVO87_000613"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7448835.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39334..40614
                     /locus_tag="IVO87_000614"
     CDS             39334..40614
                     /locus_tag="IVO87_000614"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7448836.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40619..41695
                     /gene="carA"
                     /locus_tag="IVO87_000615"
     CDS             40619..41695
                     /gene="carA"
                     /locus_tag="IVO87_000615"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7448837.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41700..44885
                     /gene="carB"
                     /locus_tag="IVO87_000616"
     CDS             41700..44885
                     /gene="carB"
                     /locus_tag="IVO87_000616"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7448838.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44939..45715
                     /locus_tag="IVO87_000617"
     CDS             44939..45715
                     /locus_tag="IVO87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7448839.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45712..46644
                     /locus_tag="IVO87_000618"
     CDS             45712..46644
                     /locus_tag="IVO87_000618"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7448840.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46641..47357
                     /gene="pyrF"
                     /locus_tag="IVO87_000619"
     CDS             46641..47357
                     /gene="pyrF"
                     /locus_tag="IVO87_000619"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7448841.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47364..47993
                     /locus_tag="IVO87_000620"
     CDS             47364..47993
                     /locus_tag="IVO87_000620"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7448842.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47981..48295
                     /locus_tag="IVO87_000621"
     CDS             47981..48295
                     /locus_tag="IVO87_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448843.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48333..48974
                     /locus_tag="IVO87_000622"
     CDS             48333..48974
                     /locus_tag="IVO87_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7448844.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49005..49709
                     /locus_tag="IVO87_000623"
     CDS             49005..49709
                     /locus_tag="IVO87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7448845.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49706..50608
                     /locus_tag="IVO87_000624"
     CDS             49706..50608
                     /locus_tag="IVO87_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7448846.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50766..52472)
                     /locus_tag="IVO87_000625"
     CDS             complement(50766..52472)
                     /locus_tag="IVO87_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7448847.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52662..53285
                     /locus_tag="IVO87_000626"
     CDS             52662..53285
                     /locus_tag="IVO87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448848.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53735..54784
                     /locus_tag="IVO87_000627"
     CDS             53735..54784
                     /locus_tag="IVO87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7448849.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54781..55662
                     /locus_tag="IVO87_000628"
     CDS             54781..55662
                     /locus_tag="IVO87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448850.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55665..56435
                     /locus_tag="IVO87_000629"
     CDS             55665..56435
                     /locus_tag="IVO87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7448851.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56714..57568
                     /gene="aroE"
                     /locus_tag="IVO87_000630"
     CDS             56714..57568
                     /gene="aroE"
                     /locus_tag="IVO87_000630"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7448852.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57746..58909
                     /locus_tag="IVO87_000631"
     CDS             57746..58909
                     /locus_tag="IVO87_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7448853.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59026..59436
                     /locus_tag="IVO87_000632"
     CDS             59026..59436
                     /locus_tag="IVO87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7448854.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59610..>61520
                     /gene="topB"
                     /locus_tag="IVO87_000633"
     CDS             59610..>61520
                     /gene="topB"
                     /locus_tag="IVO87_000633"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7448855.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCP"
BASE COUNT        20276 a        10693 c        13231 g        17320 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 accccttttt ttcttggttt cagattgggt tttcctacaa aatattttta tctttgttga
     4981 ataggaaaca aaaaaacaat ataatgaaag aaaaagaagg tgtagaaatg gataaaagac
     5041 aactgctaat agaagaatat ctggatttag ctaatgagat tattcatcgt aataaaccag
     5101 aaatggaaaa agaatttgca ggatttactc taaatgaact agaagtgatt gaacatatcg
     5161 ggaaaatccc tgatcctaac gtgacaaaat tagcgaatgc tagttacatg actagaggag
     5221 ccatcagtaa gctaacaaaa aaactgatag ctagagggat cataaacact tatcaaagcg
     5281 aagagaataa aaaagaaatc tattttcaat tgacgccaat cggacaaaaa ataaggaata
     5341 gacacgagca actccatcaa aaatgggtac agcgagatgc tactgtattt gaaaatatgt
     5401 caaaagaaga atttgacaca gtatttcgtt ttatcggtcg atacagagaa tttatacgaa
     5461 gtagcaaatg aagctactat tttttattgt agtgataata ggcaaaaatc ataagcaaaa
     5521 atccgaatac cgtcggaaga aatggtgctg cagcataaag ctgtcctgcg attagcgggc
     5581 cgatgacgcg cgatagagat tgtatacttt gggtaccgcc cattacaaga ccttgctggt
     5641 tatctgagac agaattggat agttggccat tgaatacagg agtaaaaata gaatcaccga
     5701 aaccataaca gataatccca aataaaaaca aaatcggcag tagaagaatt ccagataatg
     5761 tgatgaatag ataggcgagg atttcactga acatccctat tcttgtgatc tgctgttctg
     5821 aaaatttttt gagcaatcga ggcataatga acaattgcga gacagtatcc aagattccaa
     5881 tcaaagagaa gcttaaacca accaacccag ccttccactg aaaggcgtca atcgaaaatt
     5941 gagaaaagat agattgaaga gagccagctg ccaaccaaac agcaaaacct gctgtcaata
     6001 aaggaataac ggatgaaatc ttgaataatt gttgtaattg gtgaaaaggt cttacatggg
     6061 agaaacttaa atctactgaa cgttttttta ttggaaggct ttctagcata aatgtgtagc
     6121 cataaacggc atttaagaaa gtaaagagtg ctccaacaaa tataggaaca gaattcccca
     6181 actcagctaa tagaccccct ataataggac ccagcgttgt tcctataccg actaaagcac
     6241 ccatccatcc aaaaactttg gttctttcat taggttccgt gatgtcggca aagtaagcat
     6301 ataaggtgga gatttctcct gccgtcaatc cttcaatgat acgaccaagg aacaacatcc
     6361 atatcgaatt tcctaaacca aaaagcaaat atccgatact tgatcctaat aaactgataa
     6421 taaggatcgg atgacgccca aaatgatcac ttaagctacc gagaatgggg gcagataaga
     6481 aggcagctag agcataaaca gacatgagca aagtgatata gaaagcttgc tgatgtatgg
     6541 agtgagaata aggcagcgtt aaaaacggaa gtacaggact gacgatagta aatcctaatc
     6601 cagtgaataa gacagagacg tatccaaaaa ctttagaatg tttatttgtg aaactgtaca
     6661 tgtaaaaact cctttatgca ataatgttaa cttggaaaca aaaatagtgt atagtaattt
     6721 tgtttccttg tcaacattct aaggaacagc gaaatctact taatttattt attccgaaaa
     6781 gtgaagatac gcatcaaaaa catgaacaaa caaaaatcaa gtagacgaca ttgtaaatct
     6841 gaccgatcag tgaagtaaat agaaaagaaa cctgttcttg cttcccatgt tttttttacg
     6901 tatactgtga ttgagcggag gagaaatcat gttgaattta gaaaattact taagcgctgg
     6961 acaaacagtc atatctaatc ttttacttaa aaattatcat aggatcggtc tacaatctga
     7021 agaattttta tttgttctac agttgcatat ggcacagctt gaaggagata ctttccctga
     7081 tttgcagatg attgctatgg atatgggaat caaacaagat cagatttttc aaatactgga
     7141 tcgtctcgta acaaatggat ttatcaagat agaaacgacg attgtcaacg gtaaaaaagc
     7201 agatcgatat aatctttacc ccatctatga tctattaggt gagtatttaa aaacacaaga
     7261 aaaaaaatac gaagaaaaaa cacaagaaaa agaaatccag tcagtgtatc aattatttga
     7321 acaagaattt ggacgcccgc tttcttcgat cgagttccag cggatcggac aatggctaga
     7381 agaagaccat tatcagccgg aaattttgaa gctagcactg cgggaagccg tattaaatca
     7441 ggcttatagt tttaattacg ttgaccgcat tttgctatcg tgggaaagaa aaaatctacg
     7501 tacaaaacaa caagtggaag aagaccaaaa acgaagaaaa cagcagcttc ttcaaagaga
     7561 agtagaacag tcagctcaac aagaagaatt gccgaaagtc tcactgaaaa attggttgga
     7621 ggagtagcgg aatatgttaa gcaaacaaaa aacaatggaa gctctagaaa cgatgtatgg
     7681 aatgtttccc gaagcacatg gagaactcaa gcacaataat ccatttgagt tattgattgc
     7741 agtgatctta agtgcccagg cgacagatgt atcagtgaat aaagcaacgc cagatttgtt
     7801 tgcctctttt ccgacacccg atgcgttagc agaagcttca atcgatgaaa tcattcttaa
     7861 aataaaaacg attggtttgt atcgaaataa agcaaaaaac atcaaagcgt gtgcacagca
     7921 gctgattgaa cgtttcgatg ggcaagtgcc aacgtcaaga gaagaattga tgtctcttcc
     7981 tggtgtaggc agaaagacag caaatgtcgt attgggggat gcattcggga taccagcaat
     8041 cgcagtagat acccacgtgg aacgtgtctc aaaacgcctg aggatctgca aactagatgc
     8101 tacggtcatg gaagtagaag aaacactgat gcggaaagta ccacaagaat tatgggtaaa
     8161 gacacatcat acactgattt tttttggtag atatcattgc acggcaagaa atcccaagtg
     8221 cgaggtatgt cctttgctat caatatgtca agatgggaaa aatcggatgc gtctaaaaga
     8281 aaaggcgcta aaaaagaaac cacgcctgta gacgtggttt ctttaattgc aaataaatgt
     8341 atgaattatc ttgttattat ggagaaaagc tgtttcgttt atcttttttg catgaaaaag
     8401 taaatgtaca tactcagtag ctaggatggt aacttattac aatttattgc gataaaaata
     8461 aaagttgtta tttttattgg gaaaataccc gatgtagaaa ataatgcctt ccaatatacc
     8521 tataaaataa gtccagtatt ccaaaggatt atataaataa gtgacgtatc tatctaaagg
     8581 tgtaataaaa atgataagtg ataataaaaa aataccattt ataactaaac ataaactaat
     8641 ggatttgggc ggtttcaggc tcatcagaaa aatatagatt ctggtgaaca acaaccataa
     8701 aaaagtgaga atcattgcat gagagcttct ttccataaat tttcctttaa gagtagtgct
     8761 taaaagtaca gaaaaaataa taaatgcaag gataggagca attaaagtaa atttatttaa
     8821 taataaacaa aatttttcag cttttttatt cttggaataa cgaggtattg ttttcgataa
     8881 aaaaagatgg tgcagacaaa ttatgataat taagcatact atcagataac tcaaaatgaa
     8941 gccttctttc aaaaaattat tcaaaaccat atattgtatt ttttatttga taataacaat
     9001 gggattatta agaagtggca ctaaataaaa gactagtgtg tatttagccg atttaattat
     9061 aaacaagtaa atggataaag tatatgtaat gataaaagaa aaatggattt tctaattttt
     9121 aagctaaatg ttagatcaca catgtatatg ctggatagtc ttataaaaaa tcatgtaata
     9181 cagcttggta catgattttt atttgtagct gttttttaca tagacacaaa aaagaaacca
     9241 cgcttgtaga cgtggtttct tttttactat ctgtttccag aagctgaggt gctgctggat
     9301 ggtgtatggt catttgcagc atccgctccg ctagttgctg gctcactgga agaagcagga
     9361 ggagcagatg aagacggtgt cgagctttct tcggatgaag aagaaggaac cgttgttgaa
     9421 gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc agttgattcg
     9481 ctttgactag tagagctaga gctttgagtc gttgtctctg ttgttgagct tccaggagtc
     9541 tggacatagc tggaagatgg aatagttgtt gatggtgtaa ttgcattcga tctagctgta
     9601 tattgatctt tgtagtacag ttcaccgccc acacgaatta atccgcttgg catctcccag
     9661 tctgtatttg tcacgttagc tgaaacatac tgcataagct cgcgataaac atcagaggct
     9721 acgtgtgacg attctgaagt gacaggtgtc atttttttat tgtaacctgt ccacacggaa
     9781 atcgaataat ttggcgtata gcccgcaaat aggatatctg gatacacccc agaagaaata
     9841 ccaagttttg catactcgtc atctgtgtaa ttggatgttc cggtcttacc ggcttgatat
     9901 aacccagcga tttgcgcatt cgtaccagtt ccttcagtga tcgtatcttt taaaatgtct
     9961 gtgatcatat aagctgtttc tggagacatg gctgtttttc catctggctc ataagtttct
    10021 tctgttccgt cttggaagac gattttatta acatattgag gtttgtaata agtccctcca
    10081 ttggcgaaag ccgcataggc tgctgccatt tttaaggaag aagctccgta ttttgtccca
    10141 tcttgttctt ccgtattact agagatcgca ttcgattgat ggatcgtact gtattcgatc
    10201 ccaagatttt ttaagaaaga agccacttta tctgaaccga cttcattgaa taatttgact
    10261 gctggaacat tacgggttaa gtaaagtgct tggcgtaaag taatggtgcc catatattga
    10321 ttatcccagt ttcctacagg agtcgaagtc ccttcatagt tatacggtgc gtcagtaata
    10381 gtttttccag tagaatattt caaatattca aaagctggtc cgtaatctgt gacaggtttc
    10441 atcgtagaac caaaatcacg ggaagtattc actgctaagt tgttgcctaa agttacgtct
    10501 tctgcgatgt gacgtccgcc aatctgggct tttactttcc cggtattggt gtcgataagg
    10561 gtggaggcaa cttgcatctc gtcgtctggg taagagacat actgatccgt gttgacgata
    10621 tcatatagtt tcttttgggc gtccagatcc aaattcgtat aaatctctaa accgtctgtg
    10681 tagacattct tatctgtttt ttcttgtact tcattgatga cttctttgac ataattatcg
    10741 acgatcttag tattgtcatc ggattgagtt agttcttgca gaccatccgt taccggtaca
    10801 ttaacagctt gatcatattc tgtttgagag attttttcgt tttgaagcat cgtatacaaa
    10861 acagtatctc gtcttttctt cgcctgatct ggataaacat aaggatcata ggcggaaggg
    10921 gcttgaggca tcccagcaag caatgctgtt tgcggtaaag aaagttctga taatttctta
    10981 ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc cgtttgacat atacacttta
    11041 tttacgtagt aggtcaagat ctcttgtttc gatttttttt gttccaatct tacagccatc
    11101 cacgcttctt gggccttgcg tttcagagtc tgatcctcgg cactggtgga gaagaaggaa
    11161 agtttgatca attgctgtgt caaagtactt ccgccttgca gtccgccaga ggtaaagtta
    11221 gagagggcag aaccgatgat ccgaataggg tcaaccccga tgtgtttata aaaacggcga
    11281 tcttctaccg acacgatcgc atcttctaat gttttcggta attcattcgc agaaattttt
    11341 tcacgctttt ctgcgcctaa atcttcgaat agttcgccat cttgtgtata tagtttggaa
    11401 gatacggtag cgtctaattt tttatccgtc aattcgggcg catcttttgc gtaataccag
    11461 aaaagtccta cacctgctaa aaagaggata cagaaaaatg agagtattcc taaaaggatc
    11521 ttgataaata atcctttttt atgtgttcca gaggactttc cagaaccttt gccagaattc
    11581 ttcttcacac tctttttagg agtaggctgt ttctgtcgtc tagagcttct agtttgttca
    11641 tttgccattc ttgctttctc cttcaataga taatgttgat aaatagaagt caaccgcgtc
    11701 caaataagga atccgcggag aaataccagt tttgatctct attcctagtt cttcgatcaa
    11761 agataaaggt agtgatttgc gtcctttttc cttttgggcg tcccagtaag caatcaaatg
    11821 aacactattc aaataaaaac agcgcttcaa agacgaaaac cataacaaga caaagcagac
    11881 cccattttgt tcgaggcatg ctttcatatg ttcgatttga tgggcatgga agtttttaaa
    11941 gggaaaagat gttttgttct tggtttcttt tgcttcaaag tccaagtaaa agcctcgata
    12001 gacgccatta tagtctgtag ttgaggcttg ggtaaagtag gcttccttga tcacagcagc
    12061 acttctctta ggatagtcta ctttcacgat ttggataggg gtaggctttt tatggataac
    12121 cgccaacccg cgggaaagat aatatttatt actttcattg attgcttctt caaagcgcat
    12181 cccccgattg ccgaattcag tgatctgatt tcttttttct tgaatttttt cgacgggaga
    12241 ggcattctta taataaggtt ggccatttgg atagcgcaat accatcttta atcacactcc
    12301 taggccacat tatagcaaaa aagcggatag aaagaaaagg aatctgagat ggataagtca
    12361 aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat
    12421 cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac tccatcagtt agcagaagaa
    12481 gggctggaat gggtacttgt cagtgggaat ttgggcgtcg aactatgggc tgtgcaaata
    12541 gtagcggaat tgaaagaaga atatccggaa ctatctcttg gtattttgta tcctttttct
    12601 gattttggta ataactggaa tgaagggaac caagaaaaaa aacaattagc agaacaatta
    12661 gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca gctaaagaat
    12721 cacactcgtt ttatacttga gcatacagtg ggctgtctgc tgatctatga tgaagaattc
    12781 cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga tcatccctac
    12841 gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc gatattgtgt
    12901 ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt acgtaaatga
    12961 aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt ttacaacaag
    13021 aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc ttagacaata
    13081 tcatcaaaga ttatgaaact tacagcaaag aactccttgc tttacaagaa gaaaacgatc
    13141 gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa acacgcgtgc
    13201 aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta aaacgcttgt
    13261 caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt gcagtcaaac
    13321 cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat gataatgaaa
    13381 aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg
    13441 acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg
    13501 cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac aagctaaggc
    13561 ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg gggaaaccca
    13621 tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag gggcgctttt
    13681 tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg ttaccgcttt
    13741 gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc gcaaacatca
    13801 gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca tttgaaagct
    13861 atttttcaat acttcagatg aatcgttctt attacaaaac aaacttttgt atcaccggtt
    13921 attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt tttttataaa
    13981 taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact gcggcaagcg
    14041 ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc caagttgaga
    14101 acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat ttatggttac
    14161 gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg tttgatgaat
    14221 tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat gcagcttttc
    14281 ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc caagcgatca
    14341 caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct tctgtaccac
    14401 tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac catgtgatgg
    14461 taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg
    14521 gagcaccatt gaaagaaaac atggcagcag cacttattat gttgacaaat tggcgaaaag
    14581 accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa gcagccttaa
    14641 taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct tgggattggt
    14701 tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca gattatgata
    14761 ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt gccaaagcca
    14821 atgcagaaga aatcggatta ggctcttcga ttacctttaa gcaacaagca gtcaaagact
    14881 tcaaaacgga taaagaatac ggggtcatcg tggctaatcc gccttacgga gaacgtctag
    14941 gagaagaaga aactgttcgg aaattatata aagaaatggg cgatgtattc cgtccattaa
    15001 aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt tacggacaaa
    15061 aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta ttccagtatt
    15121 ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa ttgattcatt
    15181 caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa gaagtaaaag
    15241 aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa acaggtatgc
    15301 ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc atttattttt
    15361 caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa catccggaag
    15421 aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat gatttggaac
    15481 atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca gcacacggac
    15541 aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca ttgaatcaaa
    15601 atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag acgaattatg
    15661 atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat aaggtatttg
    15721 atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac
    15781 ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga tttgtagtga
    15841 aagtaatcga acaacttggt tatgatttta gtcgtggtcg tttagatgac actgtccacc
    15901 catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg aacgaaacag
    15961 atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg tatgaacaag
    16021 acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg ggtatccatg
    16081 aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc tggaaaaagc
    16141 aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct tttgacaaat
    16201 tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca gacagtttga
    16261 cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc aatgaagggg
    16321 tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat ctgggaatac
    16381 gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt gcaagctttg
    16441 gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt cattctttgc
    16501 agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca atcaaaaact
    16561 ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa ttaatcatgg
    16621 atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa gacctgtatt
    16681 ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat tcatctaatc
    16741 tatagatgtt ttcagctatt tacttaagaa agaagtggaa cgaatggata aattacaagt
    16801 gatcgctgat tatagtatgg accaattagc gcatgatcaa acaggacatg ggagcgatca
    16861 taccaaaaga gttgtcaagt tagctgaaaa aattttggag acggaaccgc aggcagaccg
    16921 ctttgtgacg cttgcagcag cttacctaca tgatacgatt gatgataaag tcgttaaaaa
    16981 tgagaacgag gcaaagcaac agcttcgtgt attccttcgc tctttgccaa tagtagagga
    17041 acagatctcg atgatttttt ccattatcga aaatatgtcc ttttctaaaa atctctctga
    17101 agcggccgaa ctatctctgg aaggtaagat cgttcaagac gctgatcgta tcgaagcatt
    17161 aggagcaatc ggcattttgc gtactgctta tttcggcggt ggacatgggc acccgatttt
    17221 tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa aaggcacgac
    17281 agtgatcaat catttttatg aaaaactgtt cttgctgtct gataaaatga atactgatta
    17341 cggatatgaa gaagccaaac gaagagaaac cttcatgcgt gaattcttag aagaattttt
    17401 tctggaatgg tctattgacg atgaaaattt tactccagac aattggatca aaaataacaa
    17461 aaaatgatgg aggttgtgac agaagtggtc agctccaaga tatttcggat cattttccgt
    17521 aaaagttact tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag
    17581 tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga tgaatattgt aacttgtgat
    17641 tcggaattgg caggagaaca ccgtttattc ggaagaatag gactgtgaca agacttttgt
    17701 cacagtcctt ttttcgaatt caacaagtaa aacttttaag aaatattcag agagtaaaat
    17761 taaaaattgc tgataaacga agatggaaat tttatgtatt tttattggtc tatgcaataa
    17821 ataaattaaa tattcagaat acaattgatt ttaacgaaag ggcttctatc aatggttgtg
    17881 ttgtatttat ctgcccaggt agagtaaaat agatcattat ctaaaaggta attgttcata
    17941 ctatataagt catttgaaag taaaaagata ctgtaaataa aaaatgtggt taaataattc
    18001 tcataatcag tgtaagcgtt atcaaaaata tagcatactt gctttgtaga tgaatgacac
    18061 gatatatgtt tggtatgttc tgcaagacag atgaatatgg aggtggagac aatgtcttcc
    18121 aaagaactaa caatgtattt agagaaagaa ttggatcaac tgaaagaaaa ggggttatac
    18181 aatacgatcg acatactaga aagtgaaaat ggtgcttgta tcatcgtaga cggaaaaaag
    18241 atgatcaatc tagcatccaa caattatctt ggatttgcca atcgcgaaga attgaaaaaa
    18301 gcatgtattg aagcaaccga aacttatggt gtaggagcag gagcagtccg aacgatcaat
    18361 ggttcgctga aaatccatca gcaattagaa gaaaaaatcg cagaattcaa agggacagaa
    18421 gcagcaatcg cctttcaatc tggattcaac tgcaatatgg gcgctatctc tgcagtaatg
    18481 acaaaagaag atgctatctt atcggatgaa ttaaaccatg catctattat tgacggttgc
    18541 cgcttatccg gagcaaaaat catccgaatt aaacatcaag atatgaaaga ccttgagaaa
    18601 aaagcaaagg aagcgataga aagtaagaaa tataaaaaaa tcatgtatat cacagatggt
    18661 gttttctcca tggatggaga cattgcacga ttgccggaaa ttattccgat tgttgaaaaa
    18721 tatggactga tcacctacgt cgatgatgcg cacggctcag gcgtaactgg aaaaggtgca
    18781 ggaaccgtca agcattttgg attaagcgac aaaatcgata tgcaaatggg cacgttatcg
    18841 aaagctatag gtgttgttgg gggttatgta gctggatcga aaacactaat cgactggttg
    18901 aaagctagaa gtcggccatt cctattttct acttcgctaa ctccaggtgc agctgcagct
    18961 gcattggctt cgattacttt gatgcaagaa catccggaac ttgtcgaaaa agtttgggaa
    19021 aatgctaatt attttaaaga agaattgaaa aaagtaggct ataacatcgg tatgtctgaa
    19081 acacctatta ctccagtcat tttgggagac gaaaaagtga ctcagacttt ctctaagaag
    19141 ctgatcgaac atgggattta tgctaaacca atcgtttatc caactgtgcc tcttgggaca
    19201 ggcaggatca gaaatatgcc gactgcggaa catacgaggg aaatgcttga tgaagcagta
    19261 gcagtttatc aaaaaatagg aaaagaaatg gaaattattt aactaaattt agttttttcg
    19321 tctatttaaa attagagaac atgttcacgc atatattgtt aatcatctga agatagtagt
    19381 aaaaaagatc agggaggatc atcatgaaaa aagtattggt gacaggatgt ttaggacaaa
    19441 ttggttcaga gttgatcaca cggctacgtc aagaactagg cagtgaaaat gtcattgcga
    19501 cagatatacg catgcctgaa gtcattgaga aaaacgatat ctttgagatc ctagatgtaa
    19561 tggattacga aaaaatgcgc agcatagcag aacaatatca agtggacaca ttgattcatt
    19621 tggctgctct tttgtcagct gttgcagaag caaaaccaca attagcctgg aatttgaacg
    19681 tgactgggct ggtcaatgca ttggaagtcg ctagagaatt ccagctgaaa ttttttacgc
    19741 caagttcaat cggcgcgttt ggtccaaata ctccaaaaga tcatacccca caagatactc
    19801 ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc tggagaacta ctatgtgatt
    19861 attattatgt gaaatatggc gtagatactc gtggtgtccg tttccctgga ttgatttctt
    19921 atgcaacctt gccaggaggc ggtacgacag actacgcagt cgatatttat tattcagcaa
    19981 tcaaagaagg aaagtacact agtttcattg atcaaggtac atcaatggat atgatgtata
    20041 tgccagatgc gatcgatgcc attataaaac tcatgaatgc agatcctgat cgattggttc
    20101 atcgaaatgc cttcaatata tcggcgatgt cttttgaccc ggaagagatc aaagcatcta
    20161 ttcaaaaaat cataccagat tttgaaatgg attatgatgt ggatcctgta cgtcagtcaa
    20221 tagcagaatc ttggccaaat tcactagatg tttcttgtgc gcaagaggaa tggggctttt
    20281 cgccgaaata cgacttagat gtgatgacaa aggatatgtt ggaaaaactg aaagaaaaat
    20341 tggtagagac ggtataaaac aagaaccaca tccaaaaagt tttttatact tttgggattg
    20401 gctatggtat gaaaggggac aagggatgga agaatatcaa gccacgccag taaaagaagt
    20461 aaaaatagaa aatgaattaa aacgtacgat gggatttttt actgctttat cgactgttat
    20521 gggcacagtg atcggtgcag gggtattttt taaagccgca agcgtagcag aagttacagg
    20581 atccgccagt ttgcatatgt tttcttggtt tctcggtggt atgatttctg tctgtgcagg
    20641 actaacagga gctgaactgg cagctgctat tccagaaaca ggagggatga ttaaatatat
    20701 cgaacggatt tacggaaata cagcagcttt tttattgggc tgggcgcaag tcgtgattta
    20761 ttttccagca aatgtagcag ccttgtcgat cattttcggt acacagttcg tcaatctatt
    20821 tggtctttcc cagtcaatga ttgttcccgt agctgttaca gctgctgtat cgatcctgtt
    20881 gatcaatttt ttaggttcta aagctggagg ggcttttcag tcgattacgc ttgtctgcaa
    20941 gcttattcct ttgtttgtga tcgtcatttt cggtttgttc cgacaagaag gagtagactt
    21001 ccaactgttt ccgatccaag caggagaaaa tctttcattt ttctcagcct tgggagcggg
    21061 cttgctagca acgatgttcg catacgacgg ttggatacac gtaggaaata tttcgggaga
    21121 attgaaaaag ccggcgaaag atttgccaaa agcaatttct ttaggaatca tcggaatcat
    21181 gatcgtttat ttattagtaa atgcggtgtt cttaagaacc gcatctatcg acggagtcgc
    21241 aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt tttggcggct tcggtggaag
    21301 actggtcaca gttggtatct tgatttctgt ttatggaacc atcaatggct atacccttac
    21361 tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac ctgcctttta gcaagctctt
    21421 tgctaaactt catgataaaa ctaaagttcc tgtagccgca ggtatcttag agctagtcat
    21481 cgctattggc atgatgatga tcggtggttt tgatacacta acggatatgc tgatctttgt
    21541 catttggatt ttttatacga tggtctttgt tggtgtcatt cttcttcgta aaaaagaacc
    21601 tgatttgttt cgaccataca aagttccaat gtatccgttt attccactag tagcaatcat
    21661 tggagggact tttatcgtat caagcacgct tatcactcag acctttttag cttcgatggg
    21721 gatagtattg acacttgctg gtatcccgat ttatctctat ttaaaacgta gatagctaaa
    21781 acattcgaat tttattagaa ctgcacttct ctgccaaaca aaatatttta tttaaatgag
    21841 gagtacgtgg aagaaatgtc aaaagtactc gttaattttg atcacgtaaa tgtcgttttg
    21901 tctacgatga aatagcaaag taaaagacag aaatcgggct caaccgattt ctgtctttta
    21961 ctttgatttg cgtaactgaa ctattcggcg tacgccgcga taaatattta ataaaaccaa
    22021 gcaaccagaa atgaaaatgg gaagccaaat cagatagaca gggtagccaa gcactttaga
    22081 caaagcatgt ggagtcattt tatccatttc aagaagtgct tgctgccaag tctgaagatt
    22141 gaagactagc atgaaacccg aaataatcag gagaataaat cctaaataat gaattgtagt
    22201 tctttttctt acataagcga tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa
    22261 aacaaagtac atattcagca tctcgacacc ccctttgtgt ttatcttact ttactttgaa
    22321 agaaatgaga aataatagcc tcgttacttg cttgaaacag caaaggactg ctaagataag
    22381 agagatacaa agaatcaagg aggagtcata tggcaagtta tatcgaaccg attcgttttg
    22441 cgctgattgt ttttccattt ttagccttag cgatctcatc tgtttttttt atttatgaat
    22501 atcgaaagta tggaacattt ctagtaacaa gggcagttat tttgtattct ttcgtttttt
    22561 atttattgtg tgcgtatttt ttggtgatct taccacttcc ggcaaaggag acggttgccc
    22621 aaatgacagg gccaaagatg gagctgcatt tatttgcttc atggcaacat ttcagagaca
    22681 acacggtatt ggtcttgtct gaacctagta cctacctgcc ggcaatgaag cagagtgtat
    22741 ttttggaacc agcttttaat gttttattac tcataccatt tggcatttat ttaagatatt
    22801 atttccggct ttcttttatg aaaacgatga ttgctagttt ctgcctttct ctattttttg
    22861 aattgacgca gctaagtgga ctttatttta tctacccaag accataccgc ttatttgatg
    22921 tgaatgattt agtgacaaat acattgggtg gaatcattgg ttttgtcgtt acgccgttat
    22981 ttacatttat gcttcctaca agaagacgaa tggatgaact ttcttatgaa aaaggaaaga
    23041 ctgtctcatt gactcgacgc ttggtcgctt atttgatcga ctggacattt ttatctctta
    23101 ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga ttcaggggca gttacttctc
    23161 atatctggtg gcttttatta gaagtagggg tatactttat gattgtaccg tttataacca
    23221 atggatggac acttggcaaa aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac
    23281 gaatttcttt aaaagcattg atgatccgct acgggtatct gtactttctt ttttatggtt
    23341 taagctggtt ggggcagttg cttcagcaaa tcatgaattc gacaacgaat acggtgttgc
    23401 agatgagtgc agtattcatt tctctcggtg tcttgttact acaaggtatt tttattttaa
    23461 atatcttgct atcatttttg agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca
    23521 catatactgt tagcacgatt ggccaaacaa aggatgatga ttctcgaaat tagctgagca
    23581 aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa tttaaagaga agaggtgcac
    23641 gaatgaataa ttttgatata ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa
    23701 agggcttcaa acagcccaca gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa
    23761 aaaatatcat tgggcaatct caaactggta caggaaaaac acatacgttt ttgttgcctt
    23821 tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt tgttattaca gctccaagta
    23881 gggaactagc tgcacagatc tatgaagcag ctacacaaat cgctaaattt tctacaccgg
    23941 agatccgtgt ctcgaatttt gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc
    24001 atcaccaaca gccacaggtt gtagtaggca cgcctggtag aatattagac atgatgaatg
    24061 aacaagcgct gaagattcac accgcatttg catttgttgt cgatgaagcg gatatgacat
    24121 tggatatggg ttttttaaat gaagtagacc aaattgcaag tcgattacca gaaaagctgc
    24181 agtttttagt tttttctgcc actattccag aaaaacttcg tccattctta cgaaaataca
    24241 tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt tatttctgaa gacattgaaa
    24301 attggctgat ttcaacaaaa ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa
    24361 ttggtcatcc ttatttagca atcgtatttg ccaatacaaa acagcatgtg gatgaaattg
    24421 ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat tcatggagac atcaccccaa
    24481 gagagagaaa acgagtgatg cgacaagtac agaatctgga gtatcaatat gttgtagcga
    24541 ctgatttagc tgctagaggg atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa
    24601 ttccagaaga tctagacttc tttatccatc gtgtcggacg tactggacgt aatggcttaa
    24661 aaggaacggc cattacgtta tattcgccaa atgatgagca agccattgat gcgatagaaa
    24721 aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg
    24781 atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt attagatccc acgctgattg
    24841 gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta taagaaaaaa atcgattggg
    24901 caattgcaga aaaaaataaa aaagaacgca aaatcgaacg cagacagcaa agccgtactg
    24961 ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg
    25021 tcacaatctc ttgtttttaa gctagacttt ttttcattcc gatacaaagt acttcatctt
    25081 caataaaagg ttctgtgcaa gtatgatacc cttgtttttc ataaaaatct acagccgtca
    25141 tttcagctac tacataacta gtctgacaac cttctgaaac cgcccgtttt tcagcagctt
    25201 caagcaactg accgcctaac ccttttcctc ggtattcttt tgctacgcaa aatcgttccg
    25261 gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt agctactggt cttttttgat
    25321 caagaaatag ataataggtt tgttgcggga tatcttttcc gtcgaattct agttctgcag
    25381 gaatcccttg ttctaataca aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc
    25441 attcatccgc tccaactatt tttttcatca tattcctcct tgtcatgaat agcagagatg
    25501 tattgattcc tttattctta tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa
    25561 aataaaaatt gagatattga cagtgatcaa tagaatgcgt atactaatta agtacgaaaa
    25621 ggatatgatt tgttttttaa gactcacaga aaattctttc ttggctgaaa aaagaatagt
    25681 cactaatcaa attgctccct ttttctattt tggtagaaat accgacgcag ctcagcgtta
    25741 tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg
    25801 tccttggctg tgatggtgtt tttttgtatt taatttatag aaagcaggta atgacatgaa
    25861 acaattaagt agtgcacaag ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca
    25921 tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa
    25981 ctctggtgtt gcaacattga aaaaatattt tgatggttca gttgtgccag aaaatccaag
    26041 aatcacaaat gcccaaaaat ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc
    26101 acggcaccat acgatgtttg agatgctggg taatttttcg attggtgact atttcaaaaa
    26161 tgaagccatc cactgggcat gggaattttt gactgatcca aaatggatgg cattcgatcc
    26221 agaaaaactt tatgtaacag tttatccaaa agatacagaa gcaaaacgca tctggcatga
    26281 agaagttggc ctgccactag atcacattat tgatgtagaa gacaacttct gggatatcgg
    26341 tgcaggccca tgtggtccgg atacggaaat tttctatgac cgtggagaag cttataacga
    26401 tttagcagaa gacgatcctg aaaactatcc aggaggagaa aatgaacgtt atttagaaat
    26461 ctggaactta gtattttcag aattcaacca taagcctgat aacacatttg aaccacttcc
    26521 tcataaaaac attgatacgg ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc
    26581 gtcaacgaac tttgagacag acttgtttat gcctattatc cacgccgtag aaaagctaag
    26641 caaacaagta aaatatggtg aaaacccagt aacagatatt tcttacaaag taatcgcaga
    26701 tcatattcgc gccctttcat ttgctattgg cgacggtgca ttgccatcga atgaagggag
    26761 agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat
    26821 tgaacaagca ttcttatatc gtttagtccc tgttgttggg gaaatcatgg taagttatta
    26881 tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt atccgtacag aagaagaacg
    26941 tttcaatgaa acaatcaatg aaggtttgga aatcttaaat caagtgatcc aagaagtaaa
    27001 aaacgaaaac gggacgactc tagcaggaaa agacatcttc aaactatacg acacatatgg
    27061 cttcccggta gaattaacgg aagaggtggc agaagatgaa ggactgaaag ttgatcatgc
    27121 tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct cgctctgccc gcagtactga
    27181 aaaatcaatg ggggtacaat ctgctttatt gacagatatc aaagtagaaa gtaaattcat
    27241 tggatacaca gaacaagaat cagatagtca attattggtc atcgttaaag gtgagtctct
    27301 tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt gatcaaacac ctttctatgc
    27361 agaaatgggt gggcaagtag cggatcaagg atggatcatg aatgaagtag gcgaaatcgt
    27421 agcagttgtt acagatgttc aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt
    27481 aaaagcacca atgagtgaag gacaaaccta tcatctattg atcgatcaat cgatgcgcaa
    27541 tcggatcatc aaaaaccata cagcaaccca tctactgcac aaggcattga aagaagtact
    27601 tggcgagcat gcaaatcaag ctggttcttt agtcgctcct ggacatttac gattcgactt
    27661 tacccacttt ggtcaaatca cttcagatga attagctgaa atggaaagaa tcgtcaacga
    27721 aaaaatctgg gaagctatcc cagttgaaac aattgaaaca gatatcgata cagccaaaaa
    27781 catgggtgca atggctttgt ttggtgaaaa atacggacat gacgttcgag tagtaaatat
    27841 tggcggctgg tcaatcgaac tgtgcggagg cacacatgta gcaaatacag aaaacatcgg
    27901 aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt
    27961 aacaagtaaa gaagcgtacg aactacttga agaagaagaa aaacaactaa aacaagtagc
    28021 aacaattgtt aaatcacctc aattgaaaga agttgtttca aaagcggaac aattgcaaca
    28081 gcaagtaaga gagctacaaa aggaaaatga acaattagct agcaagttag cgaatcaaca
    28141 agctggtgat gtattcaaag atccagaaac gatcaatgga aaaacaatca ttgcagcaca
    28201 agtaaaagtg aaagatatga atcagctgcg tcaattagct gaccaatgga aacaaaaaaa
    28261 tatttcagat attcttgttt tagcaaacga acaagaagga aaagtcagtc tgctagcggc
    28321 aatgacgaaa gaatcaaatg aagctggact aaaagctggc gacttgatta aagcaatcgc
    28381 accgaaagta ggcggtggcg gtggtggacg tccagacatg gcacaagctg gcgggaaaaa
    28441 tcctgctggt attccagatg cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg
    28501 cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa cgaaacatga ttatgggggc
    28561 gaagacaaac aagtcttgcc ctcatacttt tttcaattgg atggcgcatc taatcaaatg
    28621 gtcggattcg gatggtcttt agttgacgaa agtgacagaa atttttacga ttagtataaa
    28681 taaaaagggg tgaaaaaatg aatagtagaa ataagacctt tcgactagtc ttacgtgcga
    28741 tcttgttagc aatcatcatc gtacaagcga tggttccttg gctggggttt attccattag
    28801 gattcattag tttaacaatt atccatatca cagtaattat tgctgcagta gtattaggcc
    28861 caaaagatgg catggtgatt ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg
    28921 ccatgccaac aacgccattt gatacattag tgttcacgaa tccgattatc tcggtagttc
    28981 ccagagtact tgttggctta gtagcaggct tggttttcca ctggatttat caaaaaaata
    29041 aatcaataac gatcagctca gcctttgcag gggtactggg ttcattggtc aatacagtct
    29101 tagtgttagg attcatggga ttattttata caggtgcaac ggcagatgcc tatggtgtag
    29161 actctgctct tttatttaaa accttagcag gggttgcagc aatcaatggg ataccagaag
    29221 caataggtgc gggaatcata acaccattac tagcaaaagc gttatttgca gcaacaccgt
    29281 tgaaaccaga ataatgacca tcttttaata gtaataggac gcacgataga tagaaaaagg
    29341 aagagaggag aggaaaacaa tcgtcctctc catcttttta cctgagcaac aagcaaacta
    29401 gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa agaaacaaat accgcaacag
    29461 cggacaaaaa aaggagtact agcgggtatc ttaggattgg ttacgaatct gatcttgttt
    29521 gtctctaagc taatgatcgg gcttttttca ggaagtgtct ccattatggc tgatgcagtg
    29581 aacagtctat cagatactgc ttcatccata ttgactttga tcggatttaa aattgctgca
    29641 aagccagcag atcaggaaca tccttatgga cacgaacgct tcgaatatat cagtgggtta
    29701 tttgtttcga tcatcattac ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa
    29761 atcatccatc cagaaaatgt ctctttaaca cccatcgtct tttttgtcct aatcatttcc
    29821 atctttttga aatttgcaca aggcaaaatg tatcaggtta ttgcgcaatc cattgattct
    29881 gaaacattga gagctacagc aacagatagt tataatgatg tctttactac actggctgta
    29941 ttgctttcag cagcaatcga atgggtaact ggatggcgta ttgatggata tataggtttt
    30001 gctttagcat tatatatcct ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa
    30061 ctattaggaa gtcgcccaac gccagcagag attgaagaaa tggaagagca tttaaatgct
    30121 tgcgatgata tacttggcta tcatgattta ttggtgcata actatggacc caataaacgc
    30181 tttgcatcag tccacatcga agtagatgaa tcaatgaatt tgaatgtggc tcataagatt
    30241 attgatctga tagaaaaaga ctttaaaaag tctttggatg tggaacttgt ctgtcacttg
    30301 gatccaatgc ctgttgataa cgaaaaatac tataatatct tgcggcagtt aaagcaaatt
    30361 cttcttgaaa caggtacagg gcttaatatt catgatttta gagtaattca tcaaggaaaa
    30421 acgctgcaat ttgatgtagt ggtccctgag aaattccagt ttgttgatac agaactagaa
    30481 agaatcttac gtagtcaaat agaagaaaaa atcggagatt accaattgga aattacgttt
    30541 gatcacaatt atcttttata ttaaatgaga aactataaaa agactcctta aagtttttaa
    30601 tttaaagagt ctttttttct tttaattgtt tatggagagc ctgacgtgca agattatctg
    30661 ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa
    30721 gttcttggat tctttgaagg tagggaagga agagctcgtt cttcgttgcg tttttgtcga
    30781 ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt
    30841 ttttctttag aaattctaaa gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc
    30901 ctatagaaag cgggatagat agctgttctt gtttaccttg atagacaatc aagattccac
    30961 ctccactagg accagggttg ccttttgtag aggcatcaat gtatactttt aacataaaaa
    31021 gagattctcc tttttgttca aactaatgtt atggtaagat tatatcatta ctgaatcggt
    31081 gagaggttag agaatgaaaa aaaatattcg atggcagcct gaactggcac atacgatcat
    31141 ctattggtca ttgaccttga tcattctgtt ttttagtctg attctttctt tagaaaacac
    31201 aggtccttat tggaaaagca attttgtttt aggtattttt cttgtttttc tatttctagg
    31261 tttgcggcgt tcttttattt taaaatcaaa ctctgtgatt atacgttatg ctgctttttg
    31321 gaaagaccga gaaatatctg tcggacagat tcgatctttg acgccaacta atactggggt
    31381 gcaaatgatg ttgcaagatg agaaagcccc atctgttttc atgatgcgaa aaaaagatca
    31441 agtagtcctt tttgatcatt ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc
    31501 ttatacgaaa aaaagaaaaa gctaatttta aaaatcagaa cctaaaaaaa cgaattttta
    31561 caggaaaaga aaagagtgag acgtacggtt catcccgttt agcgtctcac tctatttgtt
    31621 tatctattca tgcatactag tatcggtagt ctgaacgttg cctttcttga catgggcagc
    31681 ttgtgttcct cgattccctt cgatgatctc aaattcgacc acttgatttt cttccaaagt
    31741 tttgaatccc tcttcttcaa tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata
    31801 actgataaag ccgtatcctt ttttgttgtc aaaccatttt acaattcctg tggtcaaagc
    31861 actcacgacc tttctaaagt ctgatcatag ccttcattat aaagagtatt tataagtacg
    31921 tcaaataaat taaaaacctg taattttatg ttttttttct gaataaaaaa aggtataatt
    31981 ggcatatctt aacaaagaag atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa
    32041 tgaagaatga tctgcaaatt tcacaagaaa cagagctaga accgattgtt gaagtggcta
    32101 gtaaattaga tcttaaggaa gacgatttgg aactgtatgg gaaatataag gcgaaaatca
    32161 atttttctgg aatcaatcga tcaaaggata acgctgaggg gcatttgatc ttagttactt
    32221 ccatcaatcc aacaccagct ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg
    32281 cgctcaataa aatcaataaa aaatctgtta ttgcattgcg agaaccctca cttggtcctg
    32341 tgatgggaat caaaggtggt gctgcaggcg gaggatatgc acaggtattg cctatggaag
    32401 atatcaactt gcattttact ggtgacatgc atgctatcac aacagctaat aatgcattat
    32461 ctgctctttt agataatcat attcatcaag gaaatgaatt gaacatcgat gcccgccgag
    32521 tgatttggaa acgggtagtc gatctcaatg atcgtgaact gcgtaaagta attgttggct
    32581 tgggtgggcc aatccaaggt gttccaagag aagatgggtt tgatatcaca gttgccagcg
    32641 aaatcatggc tatcttgtgt cttgcggcag accttgaaga tttaaaggct cgtcttgcaa
    32701 ggatcgttgt cggctataca tttgaccgtc agcctgtgac tgctggagac ttgaaagcag
    32761 aaggcgctct ggcattactg ctgaaagatg caatcaagcc gaatcttgta caaacaattt
    32821 acggaacacc agcttttgtc cacgggggac cgtttgccaa tatcgctcat ggctgcaata
    32881 gtattttagc taccaagaca gcattgagat tagcagatta cgttgtcaca gaggcaggat
    32941 ttggtgcaga tcttggcgga gagaaatttt tagatatcaa agtacctaac ttgaaaaaag
    33001 cgccggatgc tgtggtgatt gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga
    33061 aaaaagatga acttaaaaat gagaatcttg atgctttgaa agtaggtttt gcaaatttaa
    33121 agcgccatat acgtaatatg gaacaatatc agctacctgt gatcgtcgca atcaatgaat
    33181 tcgtaacaga tacagacaat gaactcacac tgttggagca cttgtgtgaa aatcagggga
    33241 ttcttgccaa gagagcgagt gtatgggcag atggagcaga aggcggcgtt gatttagcaa
    33301 aagctgtagt acgattgatt gatagaaaag aagctaatta caagccatta taccgcctag
    33361 aagaaacgat acaagaaaag acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg
    33421 ttgttttcag caaaaaagcg caaaaacaaa tagaagagtt tacgaagaat ggatgggaca
    33481 aactgccgat ttgtatggca aaaacacagt attcattttc tgatgatcct tcactgctag
    33541 gtgcaccgga agatttcacc atcacgatcc gcgaaatcat cccaaaatta ggggcaggat
    33601 ttttagtagc cttgactgga gatgttatga cgatgcctgg tttgcctaaa aaacctgctg
    33661 cattgaatat ggacgtgaca aatgacggag aagtgttggg attgttttaa gcttgattat
    33721 aatctaccat cgaaggagct gtattttgac tacagcttct ttttttagac gatagggaat
    33781 taattttgtt tacactagga tcattctttt tctttattgt attttgagct atactaaagc
    33841 ataataggag ggtatgatga aaacgacatt tataagaaaa accggttttt acggtatggg
    33901 tagctcgatt cagctaagaa tcaacaatga aaaacgcagt gttctcagtc atgaacaaaa
    33961 gataacgctt gatctggaag tgccctttac gatgcaggtc accttttatt ggctaaaaag
    34021 ccccgtttat acagtgaaag aagcaaagcc tgcttatgtg atcacaatga attacttgat
    34081 tcttcaaatc tatccgtttt tatttctagc aactgggttg ggggccgttt tcctacaaaa
    34141 tttatttttc agtttttttg cagttattgt gttgattgcg ttctttttct atataaaaaa
    34201 ccgggcatat ttgatcaagg aggaaaatta tggcaaactc tgatcttttt ttaatcagtt
    34261 tcaatcgaat cgaaaaatgg atgagagacg agatgggaaa tgcacgaaat atgggattta
    34321 ccgaacttgt cagacgccta gctcaaagaa aacaattaat gatcagaaaa tacgaagatg
    34381 atcttttaca gcttgctcag ttaaggaatg cgatcgtcca tgatcggatc gcagtcgatt
    34441 ttgtcattgc agagccaaat gattggacag tcaagcgaat ccagctgatc gaacaagaac
    34501 tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg tgtaacaggt tttgaacaag
    34561 atcttccgtt acttgaactg ttaaagatag tagcagaaaa aaggtattca caatttccgt
    34621 tatacaataa aggaaaattt gtagcattga ttactttacg taatatcggg ttttggctag
    34681 caaaagaaag ccaaaaaggt ccggttgatt taacaaataa acgagcgttg gatttgatca
    34741 tccaaaacgg aaagtacaca aattatcatt ttgttccagc taaaacacat atttacgagg
    34801 ttgaagcgat gtttcgtgag caagggactt tggaagccgt acttatcact aaagatggga
    34861 atcccgatgg taatctgctc gggatcgtac gacctcgtga tatttataaa caaatagaaa
    34921 aggattgatt ttttgttaat tatttattgg atattcagcg ctttgctagt aggagtggat
    34981 cagttggtca aatggtggat cactgataat ctagcgcttg gagagacgaa aaatcttata
    35041 ccaggtattt tgtcactgaa tcatattcga aacactggag ccgcatggag tatgctagaa
    35101 gggaagatgt ggttctttac tatagtgaca ctcattgcag tcgttgtgat tctcacattg
    35161 atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga ttggattgag tttgattcta
    35221 gctggtgcga ttggcaattt tatcgaccgt attcgtcttg gctatgtagt agatatgttc
    35281 cagacagatt ttatgagctt tccgattttt aacgtagcag atgtgacact tgtatgtgga
    35341 gtgatttgta tgttaattta tattatttta gatgaaaagg atcaaagaaa aaaatgacag
    35401 aacttaaaac gacgattcac catgaatctg gtaggatcga taaagtattg actaatttat
    35461 tcaccgatta cagtcgttcg caaattcagc tgtggctgaa aaatggagcg gtttctgtca
    35521 atggacagcc agtcaaagca aattacaaag taaaacaagg agacgagatt actgtcttga
    35581 tccccgaacc agaaacatta gatgtggtcc cagaaaatat cccgttgacg atcgtttacg
    35641 aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat ggtcgttcat ccttcagcag
    35701 gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca tatcaaggat ctttctacga
    35761 tcaacggcgt tattcgtcca ggtattgttc accgaatcga caaagatact tccggactgc
    35821 taatggttgc caaaaatgac caggcacacg aatcattagc tcagcaatta aaagataaga
    35881 cctctttgcg caaatatgtt gctttagtcc atggagagat tcctcacgaa aaaggaacca
    35941 tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat gcaggcgata agagaagatg
    36001 ggaagtcggc tgtcacacat tttactgtat tggaacgttt cgaaggattt actctggttg
    36061 aattaaaact agaaaccgga agaacacacc agatccgtgt gcatatgaag tatatcggtt
    36121 atccgttagc tggagatcca gtatacggac cgaagaaaac tttgaaaggc aatggtcagt
    36181 ttctccatgc gcaaatgtta ggttttacgc acccgactac aaacgaaaag atgatctttg
    36241 aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa attaagaaaa aacattgcat
    36301 tttaatttag ataacggtat agtaattaac aacgaaagca taaaacttaa tagaattcct
    36361 ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag gatacgtatg gtgcgccata
    36421 agtagattgc tcattgacat agtatagcta tcagtgtatc agtatacgct cagtctaaac
    36481 attcctcctg tcctcttagg gcaggagttt ttttatgcaa aaaagtaggg ggaatgaaaa
    36541 tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg agcattgact cgaattactt
    36601 atgagattat cgagagaaac catagtatcc aagatatcgt attggtaggt atcaaaacac
    36661 gaggaatcta cattgcatca agaattgccg aacgcctgaa gcagctagaa gatatcgata
    36721 ttcctgtagg agaactggat atcactttat accgagatga caaaaaagaa aatccagaag
    36781 aaccggaact tcattcttct gacatcccag tttccttaga aggaaaagaa gttattttga
    36841 ttgatgatgt tttgtataca ggacgaacga ttcgagcagc aatggacgca gtaatggatt
    36901 ttggcagacc aagaaaaatt tcattagcag tcctggtcga tcgtgggcat cgagaattgc
    36961 caatccgtgc agattatgtt gggaagaaca ttccaacatc tcgggcggaa gagattttag
    37021 tagagatgca agagcttgat ggacaagatc gcatcatgat tttgaaagag gaagattaga
    37081 ggagagagaa acatgtcgga gaaaaaagga tttcataatg atgacgtggt gttagatatc
    37141 cacgatcgcc caaccccagg acattgggta ggattaagtt tacaacactt gttcacgatg
    37201 ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg atccaggtat tgcgctagtc
    37261 agttcaggac taggtacatt ggtgtactta tttactacta aaggaaagat acctgcgtac
    37321 ttaggaagca gcttcgcttt cattgcagcg atgcagcttt tgatgaaaac agatggttat
    37381 ccagcgattg cacaaggagc agtcacaact ggattagtct atttgattgt tgcgctgatc
    37441 atcaaaaaga tcggttcagc atggctggat aaaatcttgc ctccaatcgt cgtcggtcca
    37501 gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag caacaaatgc tatgtataat
    37561 cagggcacgt atgatttcaa gtatattgct gtagcgctga tcacattagg attaacaatt
    37621 ttcttcaaca tgtggctaag aggttttctc ggattgattc cgattttact aggtattatt
    37681 agcggataca tcgtagcctt gctgtttgga atcgtggata ctaagccaat cgtagacgct
    37741 gcctggtttg ctgttcctaa ctttgagatt ccctttgtac aatatgaacc aaagctatat
    37801 cttggagcaa tcacaacaat ggcacccatc gcttttgtga cgatgacaga gcatatcggc
    37861 catcttatgg tattgaataa actaactaaa cgtaatttct ttgaagaacc gggtcttcat
    37921 aagacgttga caggtgatgg attagcacaa atcgtggcag cctttgtcgg aggtcctcct
    37981 gtaacaagtt acggtgaaaa cattggtgtt ctggctatca cacgtgtcca tagcgtgttt
    38041 gtgatcggtg gagctgcagt atttgcagtc attttaggat tcgtaggaaa attaagcgct
    38101 ttgattttaa gtatccctgg accagtcatc tcaggaatca gttttatctt atttggagta
    38161 attgccgcaa gtggaatgaa gattttaatc gagaacaaaa tcgattttga taaaaagaaa
    38221 aatctgttga ttgcttcagt tatcttagta gtaggaattg gcggactcgt ctttgaagta
    38281 ggcacattta cattgtcggc aatggcatta gcaactgtac ttggcatcat tttaaatctt
    38341 atcttaccag aaacttcacg aagcgaagaa aactaaggag gaaacattat gatcatcaaa
    38401 tcagaacgta tcagcttaaa gcatttattg accgttgagg cacttactga tcaagaagtt
    38461 atgggcttga tccgcagagg gcaggaattc aagcaaggtg ctaattggac tccgcaaaag
    38521 cgccagtact ttgctacaaa cctctttttc gaaaatagta ctcgcacaca taaaagcttc
    38581 gatgtagcgg agaaaaaact agggctagaa gtgattgaat ttgaagcaag cacaagctct
    38641 gtccaaaaag gggaaacact atacgataca gtactgacga tgtcagcatt aggcgtagac
    38701 gtagcggtta tccgtcatgg tgatgagaac tattatgacg aactgataca aagtaaaaca
    38761 atccagtgtt cgattatcaa tggaggagac ggaagcggac agcatccaac ccaatgcttg
    38821 cttgatttga tgaccattta tgaagaattt ggtcattttg aaggtctgaa agttgccatc
    38881 gtaggtgata tcactcactc tcgcgtagct aaatccaata tgcagatgtt aaaacgtcta
    38941 ggcgcacaag tattcttctc aggtcctaga gaatggtacg acgatgaatt cgaagtgtac
    39001 ggacattata tgccattgga tgaactacta gatcaagttg atgtcatgat gatgcttcgg
    39061 gtgcaacacg aacgccatga tggaaaagaa agtttctcaa aagaaggcta ccatcaggaa
    39121 catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac atgcaatcat tatgcaccca
    39181 gcaccagtta accgtgatgt agaattagct gattcattag tggaaggatt acagtcgcgg
    39241 atcgttcagc aaatgagcaa tggagtattc gtcagaatgg caatcttaga agcagtgcta
    39301 aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa cactcattaa aaacggtcag
    39361 atcaatacac gaaaaaatat gacgacacca gcagaaattt ggatagaaga tgggcgaatc
    39421 aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg aagtgtttga tgcaaaggga
    39481 caattgatca cacctggttt agtggacgtc catgttcatt tgagagagcc aggatttaca
    39541 tacaaggaga cgatcgaagc tgggactcgc tcagctgctc gtggcggttt cactactgta
    39601 tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag aaaagctgag acaagtatat
    39661 gacatcatta gaaaagatgc agttgtgaag gtattgcagt atgcgccaat cacagaaaat
    39721 ctgcgaagtg agaaattagt tgatcaagaa gcactgattg aagaaggtgc ttttgctttc
    39781 acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt atctggctat gaaagaagct
    39841 gcaaaaaaca ataaagccct agtagcccat acagaagatg aatctttatt atttggcggc
    39901 gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc ccgggatctt aagcgtaacc
    39961 gagtcttctc aaattgctag agatctgttg ctagctgaag caactggtgt tcattaccat
    40021 gtttgccatg tttctactaa agaaagtgtc cgtgtaatca gagacgcaaa aaaagcagga
    40081 atccacgtaa cagcagaagt ttcaccccat catcttattt tgatcgacga agatattccc
    40141 gaagatttcg gcttttggaa aatgaacccg ccgctaagag gacgggagga cagagaagct
    40201 ttgatcgaag gcttgcttga cggtacgatc gattgcatcg ctacagacca tgccccccat
    40261 ggattagaag aaaaaagcca aagtttcatg aaatcaccat ttggtatcgt aggaagcgaa
    40321 acagcgtttc aactgatcta tactcatttt gtagaaactg gtcgattcac gttggaacaa
    40381 gtgatcaatt ggttggcagt taaaccggct gagatttttg gtttgaatgc aggaacgcta
    40441 acggttggtg cccccgctga tgtagcagtt tttgatatta cacaaacttg cacgatcgat
    40501 aaagaagact ttttatcaaa aggggagaat acacctttta tcggctggaa agtaaaaggc
    40561 gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc agaagggaga ataaaagtat
    40621 ggaacgtttg cttatattag aagatggaac agttttcgaa ggaaaggctt ttggtgcaga
    40681 tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg actggttatc aagaaactat
    40741 cactgatcaa agttttaacg gtcaaatcat tacatttacc tatcctatgg tgggtaatta
    40801 tggggtaaat agagatgatt atgaatcgat ttctccaaca tgcaaaggag tagtagtaaa
    40861 agaacatgca cgtgtagctt caaactggcg aaaccaaatg acgctagatt catttttgaa
    40921 acaaaaagga attcctggaa tatcaggtat cgatacgcgc gcccttactc gcaaaatccg
    40981 tgaagtcgga acgatgaaag ctagtatagt tgatgctgga gattccttcg aacatgcatt
    41041 tgatcagctg aaagcaaccg ttctagcaac caatcaagtg cagcaagtct caactaacaa
    41101 accttatcca agtccaggaa caggtcggaa agtcattgtc attgacttcg gcttgaaaca
    41161 tagtatttta cgagaattga gtaaaagaaa ctgtaacctc acagtacttc cctataacac
    41221 agatgccgag acgattttag cgctatcacc agacggagtc atgctgacga atggacctgg
    41281 tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa aaaatccaag gaaaagtacc
    41341 gatttttggt atttgtctcg gtcaccagtt atttgcttta gccaacggtg cagataccta
    41401 taaaatgaaa ttcggacatc gaggattaaa tcacccggta cgagaagtag cgaccggaag
    41461 aattgatttt acttctcaaa atcatggcta tgcagtcgat gaagccacga ttgatccaga
    41521 aaaactcatc gtaactcatg tagaagtaaa tgacggaaca gtagaaggag tcaaacacag
    41581 agattatccc gctttcagtg tgcagtttca tccagatgct gcccctggtc cgcatgatgc
    41641 actacattta tttgatgaat ttatggaaat gatggacgca ggaaaggagc agtaaacaga
    41701 tgccgaaacg tacagatatc aagaaaatta tggtgattgg atctggtccg atcatcattg
    41761 gtcaagccgc agaatttgac tacgcaggaa cacaagcctg ccttgcctta aaagaagaag
    41821 gatatgaagt ggttttagta aattctaatc ctgcgacgat catgacggat aaagaagtgg
    41881 cagatcaagt atatatcgaa ccgatcacac ttgaatttgt atcaaggatt cttcgaaaag
    41941 agcgtccaga tgctctcctt ccaactttag gcggacagac aggcctcaat atggcaatgg
    42001 agctggcagc atcaggtatt ttagaagaat tgaacataga acttctagga acaaagcttt
    42061 ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca gttgatgaaa gaattgaatc
    42121 agccaatccc cgagtctgaa atcgtcaata cagtagaaga agctgtagat tttgcaaata
    42181 aaatcggcta tcctgtcatc gttcgtccag cctttacttt aggcggaact ggtggaggta
    42241 tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa tggattaagt ctttcccccg
    42301 ctacccaatg tttgattgaa cgaagtattg caggttttaa agaaattgaa tatgaagtga
    42361 tgagagattc ggctgataat gcaatcgttg tctgcaatat ggaaaatttt gacccggtgg
    42421 gtatccatac aggtgattct atcgtatttg cccctagtca aacattgtct gaccatgaat
    42481 atcaaatgct tcgtgatgct tctctttcga ttattcgagc acttaagatc gagggaggct
    42541 gtaatgtcca actcgctctt gatccacata gttttaatta ttatgtgatt gaagtcaatc
    42601 cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac tggctatccg atcgctaagc
    42661 tagctgctaa aatagcgctt ggattaacat tagacgaaat gaaaaatcct gttacaggaa
    42721 cgacttttgc agaatttgaa cctgcattag attatgttgt agctaaaatt cctcgctggc
    42781 cgtttgataa gtttgaaaac ggagaacggc ttttaggcac gcagatgaaa gcaactggtg
    42841 aagtgatggc tatcggtcgg aatatcgaag agtctttatt gaaagctgtg cgttctttag
    42901 aaatcggtac acaccatatc gaacttcctg agttggcaga catcacggac gatgacctaa
    42961 tccaaaaagt ggttcgagca caagatgatc gcctgtttta tgtagctgaa gctattagaa
    43021 gagggtacac catagaagaa ttagcggatt tgacaaaaat cgatttattc tttttagata
    43081 aactgttgca tatcgtagaa atcgaacagc agttgaaaca gcagccgttg gatgacaaga
    43141 tattgaaaac agcgaaacaa aacggtttta cagacaaaaa agtggcggag ctgtgggaaa
    43201 taaacgaaga agccgttcgc cagatgagaa aagaaaatga tttgattcct gtatataaga
    43261 tggttgacac ttgtgcagcg gaattcgaat caaaaacacc ttatttttat agttcctatg
    43321 aatatgaaaa tgaaagttca cgatcagaaa agccctcaat tctagtactg ggatctggtc
    43381 ctatccgtat cggacaaggg gttgaatttg actatgcgac tgtgcattct gtcaaagcaa
    43441 tccagcaggc aggatacgaa gcaatcatca tgaacagcaa tcctgaaaca gtttcgactg
    43501 acttttctat ttcggataaa ttatattttg agccattgac cttcgaagat gtgatgaatg
    43561 tgatcgagct agaacagccg ataggagtaa tcgttcaatt tggtggtcag acagcaatca
    43621 acttagccga acctttgact gctgctggtg taaaaatctt agggacaacg atcgaagact
    43681 tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct gaaagagttg gatattcctc
    43741 aacctttagg ggatactgct acaagtaaag aagaagccgt ggttatcgct tcgagaattg
    43801 gttatccagt tcttgttcgt ccaagctatg tattaggcgg acgtgcgatg gaaatcgttg
    43861 aaaaccagcg agatttagaa gattacatgg aacatgctgt gaaagcatct ccagaacatc
    43921 cagtactagt ggaccgttac ttgcttggtg gtgaatgcga agtggatgcg atttgtgacg
    43981 gtgaaacagt actcatccca gggatcatgg agcatattga gcgtgctggt gtccattcag
    44041 gggattcaat ggctgtgtat ccacctcaaa cattatctga atcgatcaag aaaacgatcg
    44101 aagattacac gatccgctta gcattaggtt tgaattgtgt cgggatgatg aatatccagt
    44161 ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa tcctcgagca agtcggactg
    44221 ttccattctt aagtaaggtg acaggaattc caatggcaca aattgcgacg aaagctattt
    44281 tgggagagaa actaaagaca cttggttatg aaaacggact ttatccagaa acaaaagaag
    44341 ttcatgttaa agcacctgtc ttttctttta cgaaattgca gaaagttgat acttacttag
    44401 ggcctgaaat gaaatcgact ggagaagtaa tgggatctga cagacaatta gacaaagctt
    44461 tgtacaaggc attcgaagct tctggattac acttgcctga atatggagcc gtattattta
    44521 cgattgcgga cgaaacaaaa gaagaagcac tagaattagc taaacgtttt acagaaattg
    44581 gctatagtct gataggaacg aaacatacag ctgcttattt cgaaaaaaat ggtctggtcg
    44641 ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa aaatgtagtc gacttaataa
    44701 gagatggtaa aacacaagtg gttgtcaata cgattgataa agatcgggga aatgcttcta
    44761 aagacggttt catcatccga agagaagcag ttgaacatgg tattccattg tttacttctt
    44821 tagacacagc agatgcgatc gttcgagtga tggaatcccg tgctttttca actaacccta
    44881 tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac cgttgcagga ggggacagat
    44941 gaaacaagaa atgatggaaa tcgttcagca aaaacagctg gcaccaaaga tttttgaaat
    45001 gacattgaaa ggtgatctag taaaagaaat gcagcaacca ggacagttct tgcatattct
    45061 cgtgccgcgt gcagacttgc tattacgccg accgattagc ttgaatcata tcgaccacga
    45121 aacaaatact tgccgtatca tttatcgggt ggaaggtgag gggactagag tcttctctac
    45181 tctttctgcc ggagaccatt tagatgtcat gggtccttta ggcaatggtt ttgatttgtc
    45241 caatcttcat acaggagacg aagtatttat aatcggtgga ggaatcggtg tccctcctct
    45301 atatgagtta tcaaaacagt tagtcgaaaa aggaattaag ccaacgcatt ttttaggttt
    45361 tgccacacat gaagtcgttt attatgaaaa agaatttaat cgtctaggag aaacaagaat
    45421 cgctactgac gatggaacat acggtatgca cggaaatgtc ggtaatttat tgttaggaac
    45481 gaagacggaa cctacagctg tttttgcatg tggttcaaac gggttattga agacagtgga
    45541 acagctgttt tcttcgcatc aaaatgtcca gttatcatta gaatcccgca tggcatgtgg
    45601 gataggggct tgctatgctt gcgtttgtca tacgccggaa gatgaaacag gaacaaaaag
    45661 tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga gaggtaatta tatgaatgag
    45721 aatcctttag cggtcaaact tcctggccta gaattaaaaa atcctatcat gccggcaagc
    45781 ggctgtttcg gttttggcaa agaatacgga aaatactatg atttgaatca gcttggatcg
    45841 atcatggtca aggcaacgac tccaaatgcc cgctttggca atcccacgcc aagagtggcg
    45901 gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa atcctggtct agacgttgtt
    45961 atgcataaac ttcttccaga attagaggag tacaatgagt tacccatcat cgcaaatgtt
    46021 gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca aaattggcga agcaccaaat
    46081 gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca aacatggcgg aattgctttt
    46141 ggaacagatt ctgatgttgc ttttcaatta acacaagcag taaagaaagt ttcttctgtt
    46201 cctgtctatg tcaaattgtc cccaaatgta acagacatcg ttccaatcgc tcaggcaatc
    46261 gaagctggag gggcagatgg gtttacgatg atcaatactc ttcttggtat gcggattgac
    46321 cttaagacaa gaaaaccgat tttagccaat caaacaggtg gactctccgg accagcaatc
    46381 aagcctgtcg ctattcgttt gattcaccaa gtagcaagta tatccagcct tccaatcatt
    46441 gggatgggcg gcgtgcagac agtggatgat gttcttgaga tgtttatggc tggagcaagt
    46501 gctgtagcta tagggaccgc gaatttcacc gatccgtata tctgtcctaa actaattaaa
    46561 gagctccctg tgcgaatgag cgaactaggc atcgaatcgc tggaaagatt aatcaaagaa
    46621 gtgagagagg aacgtgaacg atgaatcaac tagaccaacg accaattatt gcattagatt
    46681 tttcaactag gcaagaggta gaagactttc ttcgattttt cccgaaagaa gaaaaattgt
    46741 ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc tgaaatcgta cgttatctaa
    46801 aagaggctgg tcataatgta tttttagatc tgaaacttca cgatattccg aatacggtcg
    46861 aaaaagcaat gcggggattg gcaaagcttg gtgttgatct cacctgtgtt catgccgctg
    46921 gcggtattcg tatgatggag gcggcaatgc gaggattaga agaggggact ccagaaggtg
    46981 gaaaacgtcc gctacttcta gctatcactc agcttacttc tacaagtgaa gaagaaatgc
    47041 atgccgatca actaatcgaa gtttctttag aaaaaagtgt gatccattat gctagctgtg
    47101 ccaaaaaagc aggattagat ggtgtggttt cttctgcttg ggaagtagaa gcgatcaaag
    47161 aaatggcagg agacgaattt gtttgcttaa ctccgggtat aagaccagaa ggaacagtcg
    47221 caggagatca gacaagagta gtcacacctt ctcaggcaaa aaagatcggc tcaactttta
    47281 tcgttatagg tcgaccaatc actcagtcaa ctaatcctta taaagcatat caaacgattc
    47341 aaacagaatg gagctaacct aaaatgaatg ttgaacaaag tatcgcaaaa gacttattag
    47401 aaattgaagc agtattttta aatccaagta atccttttac atgggcaagt ggaatcaaaa
    47461 gcccaattta ctgtgataac cgaatcacga tgagctatcc aaaagtccga aaagagatcg
    47521 caaaaggatt agcaagcaaa atcaaagaag cttaccctga agtacaagtg attgctggaa
    47581 ctgcaacagc aggaatccct catgccgcat gggtagcgga aatactggat ttgccaatgg
    47641 tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa ccaaatcgaa ggacgtatat
    47701 ttgaaggtca gaagatggtt gtgatcgagg atttgatttc tacaggcgga agtgtcttgg
    47761 aagcagctga agcagcaaaa agagaaggtg cggatatcct tggtgttgcg gctatcttta
    47821 cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc cgaaatccct cttatgacgt
    47881 tgaccaatta ttctgtattg attgaagcag ctttagaaga tcgttatatc gatgagcaag
    47941 aattaactct gctaaaagaa tggaaaaaag atcctgaaaa ttggcagatt taagcgcgct
    48001 tgatgaagat agagaaatcc gatttgtgtc tccagaatca tcaattggaa tagcaaaaat
    48061 ccaaaaactg attgcgagaa gcatcttttc atcaggtatg aaaagaaact tctatcgtga
    48121 tcagtttttg atttttttaa aattcatttt tctaagactg gttagcagaa agaagagaat
    48181 aatctcttct cttttttcta ttatatatag attttctcta ttttttggta agggagctgt
    48241 ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt tatttaatga tatgatattc
    48301 ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt gaatgaattt attttaggat
    48361 tgaacaattt agagacgatc acaagggcac ctggattttt taaatataca gagcatacag
    48421 ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt tctaggagac attgaagaag
    48481 cgaatggaca accgatcgat tggaaactgc tctatgaaaa atcattgaat catgactaca
    48541 cagagcgttt tattggtgat atcaaaacgc cggtgaaata cgcaagcaaa gagttaagag
    48601 ggatgctgca aacagttgaa gaaaagatga cggacgaatt catcaaccaa gagattccag
    48661 ctgagttcca agaagtttat cgccgtcggt tgttcgaagg aaaagatgaa accgtagaag
    48721 gagaactatt agccatttca gataaggttg accttcttta tgaatctttt gatgaaatca
    48781 tcaaaaataa cccggaaaaa gtttataagg agatgttcat tgaggcagtg actacgatta
    48841 aaagctttga acatcggcct tctgttcagt atttctttaa agagatattt ccggaactat
    48901 tgaaccagga attttatgga aaagaggcgt ttttagaaac cgtctcaacg attgtaaaga
    48961 aagaagaaaa ataaaaatga tatgagatgc agggggaagt atagttgaaa aagaatatgg
    49021 atgtcgaatg gggctattct ggagacaaag gtccggaaaa ttgggcgagt ttatgtgatt
    49081 ggtttgcgag aggtgcggaa tttccattgc agtcgcctat tcatttgacc aagggggaag
    49141 aaaccaaaat agaaggagac acgctttcct ttcattatca aaagcaacgg tttactgaca
    49201 aggaattcaa aaacacgatt catttagtac cttttgacca actcagttat gtagagttca
    49261 agaaagaacg ctattatttg acagatattc atttccactt gccttctgaa catatcatca
    49321 acggaaaaca ggaagatatt gaatttcatt ttgttcatat gaacagcaaa aaagagaatc
    49381 tagttgtggg tgtcatgtac cagctaatgg tacaggaagg ttggctgtat aatcaagaaa
    49441 atggggaaag ttggaactta gaaaaacatg agcattgggt aaatccggat gtgttctttc
    49501 cggagcaaaa aagccatttc cattatattg gctcattgac aactccgcct accagcgggc
    49561 caatccaatg gtttgtaatg gatcatgtag gaaaactgaa ccaagagttt cttctcccct
    49621 tcgatggtca gtatgctcga ccgaacaacc gaccaatcca gcccttgaat ggaagagaaa
    49681 tttattattt cgaggagttt gagcaatgaa tattcagcag atgaaatacg tggtcgctgt
    49741 agcaaacaat ggtagtttca gagaagcggc aaagaaaatg tttgtctccc agccaagtct
    49801 ttcagctggt atcaaagaat tagaaaccga gttagggatc acgctgttta ctcgaacgaa
    49861 tcgaggggca tatttaacag aagaagggca agacttctta aatcgtgccg agaaaatatt
    49921 ggtccagctt gatacattag aaactcacta tctaacgagt gaaaagagag acaatttttc
    49981 gatcgctgcc cagcattatg atttcttagg tcctttgacc gcacagctac tcgaggaatt
    50041 caaagacgag atcaaacaat ttcggatcat ggagacgaca acagcgagag tgatcgaaga
    50101 agtcaaagag cagcacagtg agatcggcat tatttatatg aatgagcata atcaagctgg
    50161 tatcagaaga tatttacaac aaggagagct aagtggccgc agcattggaa aattcaaaac
    50221 gcatattttt ttaaggcaag gacatccatt agctgaacac gcagagatca ccaaagaaga
    50281 attgttatct tatcctcaag ttcgttttac acaggatgga aataatttcc cgtattttta
    50341 tgaagacctt atcgagatcc ctgatcaaga aacagtgatt tatacgagtg atcgaggtac
    50401 tttgatgaat atcgttcttg aaactgatgc ttacgcttca ggatctggta ttgtgattgg
    50461 agggattcgc gagcacttgc ggttaatccc tttggcagaa ggcgaactaa atgaatttta
    50521 tatcatttat tcggcgaaac gtccgctcag tgaaatcgct caaagattta tcaaagagct
    50581 tactcggtta ctggaccagc agagctaaga aaagcagatg agaacgctgt ttattgaaca
    50641 ccgttgatca aaaaagaggg gttgtgacag aattttgtca caactcctct ttttgtagca
    50701 aacaatttat aactaattgc tgttgtggga aagtacgcaa atatgattct agaaacactc
    50761 attgattacc tgttttgttt catagctgta attttttctt cttctggtgt cacgataatc
    50821 gtcttttggt tttcataaat aacataacca ggcttggcgc catttggttt tcggatatgc
    50881 tttacctgta caagatcaac cggcacttgg gcagagtatc ggtattttga aaaataggca
    50941 gctaactcgg cagcttctgt aattgtttca tctgagggct tgtcactttt aatgatgacg
    51001 tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg tttttttagc tgtcttcatt
    51061 gtcagctggt cattttgaag attgttttta ccaacaagaa tgagcgttcc gtcagaagaa
    51121 aagtattgat caggctgaga tggtttctta cgtttttgtt tttttaagtt cttctttttc
    51181 aggtatcctt ctgaagtcaa ttcttcttta attgcttcga tatccattgg gccggcaatt
    51241 tccagttgag ataagacaga ttcaagatat tggatttcat ctttggcttc ttggatttgt
    51301 tcgccaatca gcttaacagc gttcttcagc ttttggtatc gatggaaata tttctgtgca
    51361 ttttgatttg gtgtcaatgc cggatctagt gctattttga ttggccgatc ttcttcataa
    51421 taattaggta aaacaacttc ttttgcgccg cgtggtactt gtgtcagaaa agtagtaagt
    51481 agttcgccat ttctacggta attctcagcg ttttcggatt ctttgagtgt ttgttcacgt
    51541 tttttcaatt tatttttatt tcgttttagt tcattttcga tttttcgaat cagttctccg
    51601 ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat aatagctatc taataattga
    51661 ctgagtgttg gataagctgt tacagcagat gcctgttcgg acaatacctg ataagcaatc
    51721 ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag gaataggctg atggctgatg
    51781 gctgagaaaa attcgtccca tacaaccatc ttttcattag gacgttccag taatcttttg
    51841 gtcagctctt gagcgatatc gaaaccgatt ccctgaaact gacgttggat tgcttttgga
    51901 tctagttcag tttgggaaag ccgctgaaag attttttctt tctcggagct aaatgggttc
    51961 aagacctctt gttttggcgg agcaacatat tctacaccag gaagcaaaga acgataagtg
    52021 ttttgagagc ttccaatgtg tttgatcgca tccagtattt ttccagtttc tcgattaact
    52081 aagacgatcg tactatgacg tcccattaat tcaacgatca atatgatatt ttgtagatca
    52141 cccaactcgt ttcgtttagc aaagtggaaa tggatcactc gatcattttc gatttgttcg
    52201 atgtcttcta aaatagcgcc atccaaatat tttcgaagca tcatcacgaa gttaggcgga
    52261 ttatcaggat tctgataatc gatttgggta atctgtacac gagcataact tgggtgcgca
    52321 gataataata atcgatggtt tttgccgcgt gaacgaatca ccagcacgat ttcattttca
    52381 taaggctgat ggattttcga gatccggcca gatagtaacg tttcacgcaa ttcgttgacc
    52441 atcgcgtggg taaatacacc atcaaaggac atggttcatt cctcatttct tttcataagt
    52501 atattcatta taacgaaaaa aagaagaaaa accaatttgg ccgaatagta aagcaaaagt
    52561 ctagaaaaat cttggactaa ttacataaaa aacagtgttt ctatcgttga aatccaaatt
    52621 tttaggtata ataaaaaata ggagaaaaag tagaaaagaa ggtgtttgtt atttgggaag
    52681 tagttaaatc gagagtacaa gaaatgaatg aacatttctc agtgtcattc gcagaaaatc
    52741 caagtaagta ttttcgacgt cttgtttttc tgctcatctt atcattgctg ttaacagttg
    52801 caatgttttt attgccggaa aaaatccgag aagagcaagt aaatagtctc gtttattcgt
    52861 ccataccaac cagcaaattg aaaccttttg attacagcaa agctgatcag aaaatcaaac
    52921 aaagccgggc gatatcggtg atgttcagca aaccgaacgg gaaaacctat caggacatgc
    52981 ttgatgtttt taatgatcct gagcttatgg aagaattgaa tcgaccgatc ttttattatc
    53041 cgatcgttta tgatgtacat gaactggaac aaaaatataa catacgaacg gatgaagtga
    53101 catttatttt ctttgatgga ggaaaagaag caaatcggat aactgcgggt aagggaggaa
    53161 taactgattt tgataaggag ttgatccctg aactgaatcg acttccgtta gccaatatca
    53221 aacaattaga agaagaattg gatagaacgg gagtaacttc ccaaaattcc gagattgcta
    53281 attgagacat tggcgcgatg aaacggtggg tctatatagc gctattttta attatcagaa
    53341 caagtgatgt ttgatcttca aaataacaaa taaaaaatta aaaaatatag ttgacgaaat
    53401 tcaaaaattc gattagactt aaaacaattc ttaaggaaag ggtgacctgt aatgaactta
    53461 tcaaaacgta tttcagcaga aaacaaaaaa aattggcgca gctggcgtag ataagttgta
    53521 ttaatggtga gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc
    53581 tatgctagac atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa
    53641 agacaatgac cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa
    53701 taaataaaaa gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt
    53761 tgtagtaatt attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga
    53821 aaaagaaagt acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata
    53881 cgtgagccat ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga
    53941 aggattaaaa gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag
    54001 caagctagcc acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat
    54061 cgcaacacct gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg
    54121 tgctgtaacg gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa
    54181 tgtaacaggt gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt
    54241 gttgccaaat gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata
    54301 ccaagtagca caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc
    54361 ggtgccttcc actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt
    54421 tatctatgtt ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc
    54481 aaacaaagca aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact
    54541 agcaacaatc gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga
    54601 acttgcgagt ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga
    54661 tacagtgatc aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa
    54721 ggaaaaagcc aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa
    54781 atgatcgtat cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc
    54841 atgacttatc ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt
    54901 gctgctgtct gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta
    54961 ggcgtaggtg ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag
    55021 ataccggtta ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt
    55081 gtgatgcgaa caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat
    55141 cgctttgggt tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc
    55201 ttgattgctg tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca
    55261 ggagacaacg aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta
    55321 ggtttaactt tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac
    55381 ggatatgcag atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc
    55441 atcggtgaag tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc
    55501 ggcagtatca tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact
    55561 tatctgaagt tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag
    55621 aaagcactga acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga
    55681 caatcaaaaa tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg
    55741 atgaaattaa ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg
    55801 ctggaaaaag cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga
    55861 tctcttttaa agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt
    55921 ctcgagtttt ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt
    55981 tgctacttgc acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc
    56041 aaaaagaaga ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg
    56101 atactccagc gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga
    56161 cgatcacagc tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa
    56221 cagctaaagc attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc
    56281 tcatgatcac gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc
    56341 aaaaagggaa aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg
    56401 accttttgca gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt
    56461 agaaggactt gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa
    56521 acaattaaac atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa
    56581 tagaatattc tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct
    56641 agacgtttcg tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca
    56701 aaggaggaat aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc
    56761 ttctcacagt ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc
    56821 ggtgtattta gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg
    56881 aacttttgat atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta
    56941 tctggatgag gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga
    57001 aaagggtcgt ttgattggct ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga
    57061 agctgggatt tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa
    57121 ggcagtcact gttcaagcag cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa
    57181 caaccaaaca ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg
    57241 taagataaaa gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag
    57301 ctgtcttcta gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct
    57361 cttagatata agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag
    57421 agaaacacgt ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg
    57481 catgttgatc taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat
    57541 ccatgtgatt gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc
    57601 gcataaacgg tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt
    57661 ttattaaatt atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat
    57721 cagtaagaaa cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa
    57781 gaaggcgaaa cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg
    57841 aacgcgtatg gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt
    57901 tgaacatttg aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca
    57961 acaaaaaaat aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag
    58021 aaagcgatcg tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg
    58081 tatctcattt tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac
    58141 ttcagaaacc tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg
    58201 aagtgataca cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga
    58261 tggtccttcc cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc
    58321 aggggtagga caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg
    58381 tcaaacttta gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa
    58441 atcgtttgaa aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa
    58501 agatctgaat ctggatggtg tagatattaa aaaagggggc cagaagatgg atggacatgt
    58561 tcttttgcag tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg
    58621 ccaacagcaa gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg
    58681 tgcaccttat gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt
    58741 cttgacgaaa aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt
    58801 gccagtagac ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat
    58861 tgataatcaa atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag
    58921 atcaagaaga agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa
    58981 aacgctttct tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg
    59041 aacttcaaaa atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa
    59101 gcatcaggta tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa
    59161 gaagcaaaaa atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt
    59221 gcagatacgc cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc
    59281 atccagacaa gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt
    59341 aagattgaga tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat
    59401 cgatttggca tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg
    59461 aaacatcaag cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga
    59521 ctatacggta tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc
    59581 gcaaaaaatt aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt
    59641 cacaagcaaa agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg
    59701 aaatcagcga tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg
    59761 tagatatggt tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt
    59821 tgcctatttt cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt
    59881 cgatcgtaaa acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc
    59941 gagaaggtga agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa
    60001 agaactattt acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta
    60061 agaatctcca acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga
    60121 acgctgattg gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa
    60181 aagggattga tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt
    60241 atcagctgca agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt
    60301 taattacgac aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa
    60361 cccaagaaga gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg
    60421 gacggattct tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc
    60481 tatctagttt gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg
    60541 atgctgctca aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt
    60601 acattacaga aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt
    60661 taggaataga agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga
    60721 aaaaagttca agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct
    60781 ttgcgaagct ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg
    60841 ctatgtttgc tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat
    60901 taaagctaaa aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca
    60961 tcaagtcaaa aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag
    61021 aagtagaact gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa
    61081 cgttgatcac agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa
    61141 ttctcaaaga agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc
    61201 ttaagcagaa gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa
    61261 tcactctttg taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc
    61321 gctgggaagg gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag
    61381 agaatatcaa aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg
    61441 tagacttttc agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg
    61501 atcaattagg aagatgtcca
//
LOCUS       DACCQP010000009        58565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCQP010000009 DACCQP010000000
VERSION     DACCQP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.009"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..533
                     /locus_tag="IVO87_000634"
     CDS             <1..533
                     /locus_tag="IVO87_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7448856.1"
                     /translation="YGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEM
                     VGSLNHLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVT
                     AESSLDGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIK
                     QEIEAKSSITNLKQSF"
     gene            complement(644..2575)
                     /locus_tag="IVO87_000635"
     CDS             complement(644..2575)
                     /locus_tag="IVO87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7448857.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(2593..3507)
                     /gene="pfkB"
                     /locus_tag="IVO87_000636"
     CDS             complement(2593..3507)
                     /gene="pfkB"
                     /locus_tag="IVO87_000636"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7448858.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(3504..4256)
                     /locus_tag="IVO87_000637"
     CDS             complement(3504..4256)
                     /locus_tag="IVO87_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7448859.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(4416..4625)
                     /locus_tag="IVO87_000638"
     CDS             complement(4416..4625)
                     /locus_tag="IVO87_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448860.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4636..5964)
                     /locus_tag="IVO87_000639"
     CDS             complement(4636..5964)
                     /locus_tag="IVO87_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448861.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYQDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(6329..6916)
                     /locus_tag="IVO87_000640"
     CDS             complement(6329..6916)
                     /locus_tag="IVO87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7448862.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6999..8249)
                     /gene="clpX"
                     /locus_tag="IVO87_000641"
     CDS             complement(6999..8249)
                     /gene="clpX"
                     /locus_tag="IVO87_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7448863.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(8496..9785)
                     /locus_tag="IVO87_000642"
     CDS             complement(8496..9785)
                     /locus_tag="IVO87_000642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7448864.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9939..10871)
                     /locus_tag="IVO87_000643"
     CDS             complement(9939..10871)
                     /locus_tag="IVO87_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448865.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(11028..11687)
                     /locus_tag="IVO87_000644"
     CDS             complement(11028..11687)
                     /locus_tag="IVO87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7448866.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11690..12715)
                     /gene="sppA"
                     /locus_tag="IVO87_000645"
     CDS             complement(11690..12715)
                     /gene="sppA"
                     /locus_tag="IVO87_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7448867.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12728..13018)
                     /locus_tag="IVO87_000646"
     CDS             complement(12728..13018)
                     /locus_tag="IVO87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448868.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            13427..15232
                     /gene="glmS"
                     /locus_tag="IVO87_000647"
     CDS             13427..15232
                     /gene="glmS"
                     /locus_tag="IVO87_000647"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7448869.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(15304..16659)
                     /locus_tag="IVO87_000648"
     CDS             complement(15304..16659)
                     /locus_tag="IVO87_000648"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7448870.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16683..17852)
                     /locus_tag="IVO87_000649"
     CDS             complement(16683..17852)
                     /locus_tag="IVO87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448871.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17849..18736)
                     /locus_tag="IVO87_000650"
     CDS             complement(17849..18736)
                     /locus_tag="IVO87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7448872.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18925..22605)
                     /gene="nifJ"
                     /locus_tag="IVO87_000651"
     CDS             complement(18925..22605)
                     /gene="nifJ"
                     /locus_tag="IVO87_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7448873.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELQKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHAPAYLHSYNLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVIPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            22803..23324
                     /locus_tag="IVO87_000652"
     CDS             22803..23324
                     /locus_tag="IVO87_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7448874.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(24023..24588)
                     /locus_tag="IVO87_000653"
                     /pseudo
     CDS             complement(24023..24588)
                     /locus_tag="IVO87_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24767..25423)
                     /locus_tag="IVO87_000654"
     CDS             complement(24767..25423)
                     /locus_tag="IVO87_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448875.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(25991..26926)
                     /gene="whiA"
                     /locus_tag="IVO87_000655"
     CDS             complement(25991..26926)
                     /gene="whiA"
                     /locus_tag="IVO87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7448876.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(26960..27958)
                     /locus_tag="IVO87_000656"
     CDS             complement(26960..27958)
                     /locus_tag="IVO87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7448877.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(27955..28839)
                     /gene="rapZ"
                     /locus_tag="IVO87_000657"
     CDS             complement(27955..28839)
                     /gene="rapZ"
                     /locus_tag="IVO87_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7448878.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(28974..29705)
                     /locus_tag="IVO87_000658"
     CDS             complement(28974..29705)
                     /locus_tag="IVO87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7448879.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(29709..30974)
                     /locus_tag="IVO87_000659"
     CDS             complement(29709..30974)
                     /locus_tag="IVO87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7448880.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(31237..34053)
                     /gene="uvrA"
                     /locus_tag="IVO87_000660"
     CDS             complement(31237..34053)
                     /gene="uvrA"
                     /locus_tag="IVO87_000660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7448881.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(34063..36057)
                     /gene="uvrB"
                     /locus_tag="IVO87_000661"
     CDS             complement(34063..36057)
                     /gene="uvrB"
                     /locus_tag="IVO87_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7448882.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            36316..37818
                     /locus_tag="IVO87_000662"
     CDS             36316..37818
                     /locus_tag="IVO87_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7448883.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37820..38557
                     /locus_tag="IVO87_000663"
     CDS             37820..38557
                     /locus_tag="IVO87_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7448884.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(38602..38775)
                     /locus_tag="IVO87_000664"
     CDS             complement(38602..38775)
                     /locus_tag="IVO87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7448885.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            38751..39416
                     /locus_tag="IVO87_000665"
     CDS             38751..39416
                     /locus_tag="IVO87_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7448886.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(39498..40664)
                     /gene="dnaJ"
                     /locus_tag="IVO87_000666"
     CDS             complement(39498..40664)
                     /gene="dnaJ"
                     /locus_tag="IVO87_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7448887.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(40815..42644)
                     /gene="dnaK"
                     /locus_tag="IVO87_000667"
     CDS             complement(40815..42644)
                     /gene="dnaK"
                     /locus_tag="IVO87_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7448888.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(42695..43258)
                     /gene="grpE"
                     /locus_tag="IVO87_000668"
     CDS             complement(42695..43258)
                     /gene="grpE"
                     /locus_tag="IVO87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7448889.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(43352..44395)
                     /gene="hrcA"
                     /locus_tag="IVO87_000669"
     CDS             complement(43352..44395)
                     /gene="hrcA"
                     /locus_tag="IVO87_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7448890.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(44629..45795)
                     /locus_tag="IVO87_000670"
     CDS             complement(44629..45795)
                     /locus_tag="IVO87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7448891.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(45811..46038)
                     /locus_tag="IVO87_000671"
     CDS             complement(45811..46038)
                     /locus_tag="IVO87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7448892.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            46522..47112
                     /locus_tag="IVO87_000672"
     CDS             46522..47112
                     /locus_tag="IVO87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7448893.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            47302..48015
                     /locus_tag="IVO87_000673"
     CDS             47302..48015
                     /locus_tag="IVO87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448894.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(48130..49140)
                     /locus_tag="IVO87_000674"
     CDS             complement(48130..49140)
                     /locus_tag="IVO87_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7448895.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(49177..49659)
                     /locus_tag="IVO87_000675"
     CDS             complement(49177..49659)
                     /locus_tag="IVO87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7448896.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            49809..50177
                     /locus_tag="IVO87_000676"
     CDS             49809..50177
                     /locus_tag="IVO87_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7448897.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            50642..51094
                     /locus_tag="IVO87_000677"
     CDS             50642..51094
                     /locus_tag="IVO87_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448898.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(51218..52069)
                     /locus_tag="IVO87_000678"
     CDS             complement(51218..52069)
                     /locus_tag="IVO87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7448899.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(52082..52867)
                     /locus_tag="IVO87_000679"
     CDS             complement(52082..52867)
                     /locus_tag="IVO87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7448900.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(53219..54160)
                     /gene="ribF"
                     /locus_tag="IVO87_000680"
     CDS             complement(53219..54160)
                     /gene="ribF"
                     /locus_tag="IVO87_000680"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7448901.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(54164..55087)
                     /gene="truB"
                     /locus_tag="IVO87_000681"
     CDS             complement(54164..55087)
                     /gene="truB"
                     /locus_tag="IVO87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7448902.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(55455..57817)
                     /locus_tag="IVO87_000682"
                     /pseudo
     CDS             complement(55455..57817)
                     /locus_tag="IVO87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            58265..58438
                     /locus_tag="IVO87_000683"
     CDS             58265..58438
                     /locus_tag="IVO87_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7448903.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
BASE COUNT        16595 a        12553 c        10093 g        19324 t
ORIGIN      
        1 tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag gattttgtcg
       61 ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca gcaattattt
      121 tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg gctgctcttg
      181 ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca ttattggaac
      241 gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact tttgattttc
      301 cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat tggcctaaaa
      361 atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac ggtgatacga
      421 tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg ccaagaatca
      481 agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt taacgaacat
      541 ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat gattctataa
      601 aaaaagaaga ggctgggaaa acccagcctc ttctttttta tttctatgct tctgctactg
      661 caggagcttt tctcaataaa ccataaatca caccagatac gactgctccg atagcaataa
      721 acaccaaata catgaccggg tgactcagta agaatacaac gaaaatcccg ccgtgaggag
      781 ccagtaattt gattccgaaa gctccgacta atccgccagt taaagcagaa ccaataataa
      841 aacttggaat ggcacgaatc gggtcagctg ctgcaaatgg aatcgatcct tccgtgacaa
      901 acgatagtcc catcacgatg ttcgtcaaac cagcatcttg atctgttttg ctgaatttat
      961 tcttaaacaa acgtgtagca acgaaaattg ccaatggagg taccattcct ccagccataa
     1021 cagaagccat cacgacactt ccgccagttg caacagtcgt agctaaagtt gctgtaccaa
     1081 acacataagc ggctttgttg attggaccgc ctaaatctgc cgccatcatt ccagctaata
     1141 atgcaccaag taaagcagca ttcgaaccac tcatacttgt caagaaaccg ttcaatgcat
     1201 cattgattgc tttcataggg acattgacca gcaacatcaa gaaaccagta atcagtaagc
     1261 caaacactgg ataaaagagg atcgttttaa ttccatccaa tgattttggt aagttcttga
     1321 ataatttacg taaaaatacg atgacataac cagctaagaa tccaccaatc agtgcaccta
     1381 agaatcctgc accgcctgta ttagctaatg caccagcagc aaatccaacg atcaaaccag
     1441 ggcggtcacc gatacttgaa gcaataaatc ctgctaatac cggcagcatg aaaccaaatg
     1501 cagcttgtcc gatttggttg aaccagctag cagcttgatt gtaattccct agatttgata
     1561 attgatcatg tggcacgcca ataaattggt cgatcatgaa agaaagcgca atagcgattc
     1621 ctccaccaat aacgaaaggt aacatgtgtg atacgccgtt catcaaatct ttgtaaattc
     1681 tttgtccgat agtaccatcc gcatttcctt cttctgaact gctttctttt ccatcaccat
     1741 ggaatactgg tgcagtacca ctaagggcta gattgatcaa ctcttctggt ttacggatac
     1801 catcactgac aggacgattg atcagttctt ttccgtcaaa gcgattcatc tctacttttt
     1861 tgtctgctgc aacgattaca ccttctgcac gggcgatgtc ttcagcagtc aatcggtttt
     1921 tgattccttc tgaaccgttc gtttcgactt tgatctccac acccatttct ttcgcttttt
     1981 tcttcaaagc atcttctgcc atgtaagtgt gagcgatccc tgttggacaa gctgtaacgg
     2041 caacaacgaa ttttctttct ggatgagcag aagcttctgc agcttgtgtt tcttgttctg
     2101 ctttttcttc tgcttctttt tgctcttcag ctgtttggaa caagtcttgg acttcttctg
     2161 gtgtatttgc ttcttttaat tttcctacga aatctgcatc aatcagcaat cgagatagtg
     2221 ctgctaatgc ttgcaaatgt gtgtcgtttg cgccttctgg agctgcgatc atgaaaaaca
     2281 gataagttgg ctgtccgtct aaggcttcat aatctactcc tttttggctt tttgcgaata
     2341 aaactgttgc ttctttaact gcactgtttt tcgcgtgagg catggcgatt ccatcaccaa
     2401 gacctgtaga agtttgtgct tcgcgggcta gaattccttc tttatatgtt tcgatatcgg
     2461 aaatccggcc ggcatcaaac attttttgga ccatttcatc gattgcgcct tttttatcag
     2521 tcgcttgcaa atccatgatc atcgcttctt tgaccattaa atccttgata ttcatgatta
     2581 tcctccttga aactaatcaa tttttgtgat ttcaacttct ggcagcaact catcaatcaa
     2641 ttcgcgagct gccaagtcat cagaaaaagc tgttgcgctt ccgcaagcaa ctccccattt
     2701 aaaggcttcg attggatctt tcgtacggct gtaagcccca ataaagccag caatcattga
     2761 atcccctgca cctactgaat ttttcaatgg gcgaactaat acattcgttt tataagttcc
     2821 ttgttccgtg aagagcaaag caccgtctcc agccattgag atcagcacat tctgtgcccc
     2881 ttcagctagt aattttttcc catacggaat gatttcttct acagattgga aggttgtgtt
     2941 gtaaagctca gctaattcgt gattgtttgg tttgatcaac aacggtttct taacaagtgc
     3001 attttttagg tcatcacctg ttgtatcaat cacgaaatct gcaccttttt ctcgaatgat
     3061 atcaatcagt tcttgataaa atccttttcg caggctagct ggcgcgcttc ctgacaatac
     3121 gacgatatct ccttgtccta ctgttcctaa tgcttgcttc aactcatcga tttcttcatc
     3181 tgaaatagat gggcctaagc cattgatctc agtttcagtg tctgatttta gtttgatatt
     3241 gattcttgta tcttctttta cggttgtgaa caaagactgg atttgttctt tttcaagcca
     3301 cttcgaaata aattcgccag tgaaccctcc taaaaaccct agtgctgtag attgagaatc
     3361 gatccgtttc aaaatacggg aaacattgat cccttttcct ccaggtaact tgaagtcttc
     3421 cttcattcgg ttcaaatcgc ctagttttaa cccatctacg cgtacgatat aatcaattga
     3481 tggattaagt gttactgtgt aaatcattgt attgcctcct taattgttgt cttttcacgg
     3541 aattcattaa cgaattctaa tggacaacgc tcggttatga tcgtggctgc ttccaaaggt
     3601 gcaacagagg tgaaggacaa ctggttgaat ttggtatgat caatcaatac aaatgtctct
     3661 tcggaatttt tcatggctaa acgtttaaca gctgcctctt ctggatccgg agtcgtgaat
     3721 ccttgttcaa gatgggcacc gttcattccc ataaatgctt tattaaaatg aaaatttttt
     3781 agttgttcta tcgcattaga tcctaaaaca gcattagttg ccagctttat cgttccacct
     3841 aaaataatgg tagcaagctc caaatcaact aaatttgccg catgttgaac ggaatttgtc
     3901 actactgtga tctgttttcc ttgcagaaaa ggaatcattt ctaacgtagt agagccagcg
     3961 tctaaataaa tgacatcttc gtcattaatg gtttcagctg ccgatttggc gatttgctgt
     4021 ttttcgtgaa cgtttttgag tgatttttca ctcatattct gctcaaaatt caataattgc
     4081 tctttcttag ctccgccatg aactctttct agaaacccac tattctccag ctcttgtaaa
     4141 tcacgtctta tcgttgattc agaagcattc aaaaggttta caagatcttg tgatttcacg
     4201 attttctgct gttcaagcaa gtctaggatt ttttgtctgc gttcctctgt aagcattttc
     4261 acgatcacct caatacaaat aatagcatat tttttttcta ctagcaatat aaatcagtca
     4321 aaaacgttca taaaaattat aaatcagtca aaaaaaaaat aaaaaaccag tcagaaagac
     4381 tggtttttta tttttttgtc tatcatcaag ccgttttatc gttttttatt ttcgcttctt
     4441 gtttttttca tataaacaga agcaggtttt ctttccttat gaagaagata ataataaagt
     4501 cccatcccgc caaacatgac caaaaaaccg atgactgatc gaataggaaa attaggaaca
     4561 aaaaagagac agacggataa taatgatgcc ggaaccatta ttcgataata gagttgttta
     4621 tccattttct tcacctcata ttttttgtaa agtaatcaat tgttctaatt ctcggtaatc
     4681 taaaaattgc acacgcagat gctgctcacc tttcttgaca tcgaattgga tacttcgccg
     4741 ctttttcttt attcgtttga aggtaacatc agagagtcta tgtttgattt cctttaacgg
     4801 taattctgac agggattctg aaggaacata gattcttaag gataaaagat actcttgata
     4861 tttttcatct gttacctcta cttctagata ttccctaata taataacaat aagcttcgat
     4921 tccattatcc aaagcaacag tcggatcgat aactttatag cacatcatgc gtgatcgatt
     4981 ctcgtcaacc caaggaaaat acgcctgagc atttagtgtt ccgcgaacac gcctttcttt
     5041 ataatctcct tgttggatat cataactttt tgttacaaaa aatgaagcat tctttttagg
     5101 aacaggttgc atttgtgcat tctgatactg gacagattgg atcgtatggt cagtgtaaat
     5161 gtattcaacg aatggcggtt tcaataaacc gacaccattg tcccaagcaa gatgatcaaa
     5221 cccaagctgg tccctaaaat aggattgaaa catttgattg tttggctttt gccagttaaa
     5281 ggggtcttga taaagctgga tacatatgac ttcgttttct aattccttca ctttttctac
     5341 ttgttggatg tcgagaaaaa tcggtttagg gatgatttga tgataatagc gactataata
     5401 catttcccaa cacgaagtga aacataaacc ttggtaaaaa agatcgtatc ctggaaattg
     5461 gttgcaatcg actactactt ccccgttttg ttgtttcatt ttaggtaaat gttcgatttc
     5521 atcctttttc tcaaacaata gccgttgaga gatctgtttt gtattgtgat agataaactc
     5581 gctataagct cgcatatagg cgaacacccc atgagcaatc atttttttct gaatgaagtc
     5641 tcgaatcaaa aattgctttt ctttgaaaac agagatagac aatacatttt ttatcaataa
     5701 ttgattatct tccaagcgaa aactacattc gttctcgtca tttcctacaa ctaaccaact
     5761 ttcgtctctt accgatgcta gcattatttt ttcttgaagc tttcgaagat catacggatc
     5821 cgcttgttgt gtgaaagagc ggataaatgt tggctggatc aacagacgtg tcaaaatagc
     5881 ccaatcgctt atttcctgtt ttttttcttc ttcatttaag tctgtgagct ggatagtcat
     5941 caataattta tcttcgccaa tcatttcctc acctctttca aatcttttta taatttaatt
     6001 ataaccaatg ataaagaaaa actaaaactt tatgtcttag aaaccaattt ggacgattac
     6061 atgatagact aagtaaaaag aaacaaagag gtgtttacaa tggggttgaa atttgaacgt
     6121 acagataatt tagataaact tttcgaatca tttgctgtcg atccggaaaa aacagttgca
     6181 ccaaaagaag agatcgatcg atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac
     6241 aagaaacaaa agtaagttaa gctcaaaaaa agacttacca gccaaacttt tatgaaagag
     6301 tttgactggt aagtcttttt tattcttttt atgattcaat tgcttgttcg attgctgccc
     6361 acgcttcttc ttttccctgt tttgtaacgg aagaaaagag aataaacgtg tcattgcgat
     6421 caaaatccaa cgcttttttg atttcgctct catgcttgtt ccatttacca cgagggatct
     6481 tatctgcctt agtggcaaca acaattacag ggatatcata atacttcaaa aattgataca
     6541 tctggatatc ttcttgagaa ggcttatgac gcaaatctac taaagaaacg accgcacgta
     6601 gctggtctct agtcgttaaa tatgtctcga tcatctttcc ccattttgct cgctcagttt
     6661 tggatacttt tgcataccca tatcccggaa catcgacaaa atgtaatgcg ttctcgatca
     6721 aataaaaatt caatgtctgg gtctttcctg gcttaccgga agtacgggct aaattctttc
     6781 gatcaatcaa tgtgttgata aaagaagatt tccctacatt tgatcgtcct gccagagcta
     6841 tttctggcaa ttgtgtatct ggatattgtt ttggtgccac tgcacttatg ataatttcag
     6901 cttgatggac tttcatggtc tctctcctta acaaggtgat gaataaagca agcggctgtc
     6961 gcaaaagcca aaatgacttt tacggcagcc tcttttcatc aacccgcttt tttctctgaa
     7021 tctttataga ttactgttgg ctcaccagac aattcagctg cttcttttgt gataatcact
     7081 ttttcgatgt tttcatttga agggatatca aacatcacat ccatcatgat ttcttcaatg
     7141 atggaacgaa gtcctcgagc accggtattg cgttcgattg cttttttcgc aatggctctc
     7201 aacgcttcag gttcgaattc aagttcggta ttatctagag aaagaagttt ttgatattgt
     7261 ttgactagcg catttttcgg ctcagtcaag atccgtacca gatcatctgt tgtcaatttc
     7321 tctaaagctg ccataacagg taatcgtccg ataaactcag ggatcaatcc gaattttagt
     7381 aaatcttcag gaatgatatg ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt
     7441 gtaccaaatc caatcgtctt ttcgcccata cgattcttca caatcgtttc gatgccatca
     7501 aatgcaccac ctacgatgaa caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt
     7561 ggatgtttac gtccgccctg aggtggcaca cttgcaacag ttccttctag aattttcagc
     7621 aaggcttgtt ggacaccttc gccggaaacg tcacgagtaa tagatacatt ttcgctttta
     7681 cgcgcaattt tatcgatctc atcaatatag atgatccctt tttctgcaag ttccacattg
     7741 taatcagcag attgaagcaa tttaagcaaa atgttttcta catcttcccc cacataacca
     7801 gcttctgtca aacttgtcgc atctgcgatt gcaaaaggta cattcaatgt ttttgctaat
     7861 gtttgtgcta aaaatgtttt accagaccct gtaggtccga tcaagcagat attgcttttt
     7921 tgcagttcaa catcatctgt gttttcttgt gcttgctgat tgacacgttt atagtgattg
     7981 tatacggcaa cagctaacga gcgttttgcg cgatcttgtc caatcacgta gttattcaat
     8041 acttcaagaa tttctagtgg tttagggact tcagtgaatt cgcgtacagc ttcttcataa
     8101 aattcttcat caatgatttc tttacatagg tcgatacatt cattacagat gtagacccct
     8161 ggaccagcta cgatcttttt cacttcttct tgggttttcc cgcaaaaaga acagcgtacg
     8221 gtctcattgc tacttggatt gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg
     8281 caagaaacta tcaaagactt tacagtcttt tactatcata acatagttta gtaaaaagaa
     8341 aaagtgttta acaaaggatc aactgaccta tacctttgca ttttttactc ttaacaaaaa
     8401 ataaggaact gtgacaaaat cgtgtcacag tcccttattt cgaataaaca attttctaga
     8461 agtagcttct tcagaaatga tttattactt gatttctatt ctttcgcttc tgtcgctgta
     8521 cctgtgatca attcgattgc ttttttcatt gtcacatcat gttccagcat atcttctgtc
     8581 aacacgcgtt tgatttgttc gattggcatg ttgtatgttt ctgataattc ttggatttct
     8641 gcattgattt cttcttctgt tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca
     8701 agatttgttc ttgtgcgcat ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct
     8761 gtagaaccag ttaattgata gtacatttca ggagagatac cttgacgttg catgttgttc
     8821 aagaattcgt ccattgaacg atgtacttca tcatgaacca tcacgtgtgg caattcaacg
     8881 atttctgcgt tttcaacagc catacggatc gctgcttcat cttttgcatc gtccgctgct
     8941 ttttcttttg tttctactaa ttctttgcga tatttttctt tcaattcgtc taatgttgat
     9001 acttcgtcat caacatcttt tgcgaattcg tcatctaatt caggcacttc ttttgctttt
     9061 acttcatgga ttgtcacttt gaagactgct tcttttccag caagatcttc tgcttgataa
     9121 tcttctggga aagttacatt tacatctaga ctttcacctg atttatgacc aactaattga
     9181 tcttcaaaac ctggaatgaa tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt
     9241 ccgccatcaa acgcttcgcc atctacaaag ccttcgaagt cgattactac tgtatcgcca
     9301 ttttcagctg cagcatcttc tttgataact aattcagctt gtgcttcttg ttcacgtttg
     9361 atacgtgcat ccacgtcagc atctgttact tcacggtctt gtttttcaac aactagatct
     9421 ttatattgtc ctaattttac ttctggtttc actgttactt cagcagtgat tacccagtct
     9481 tgaccttttt ccatgctttc aacatcgatt tttggttgag aaacaggatc gatgccagct
     9541 tctttgacag cagcttcata agcttctggt aatacagcat tcaatgcatc ttcatataaa
     9601 gcttcttcac cgtacatacg gttgaatact tgacgagaaa ctttcccttt acggaatcca
     9661 ggtacgttca agcttttttt cactttatta aacgcagttg ttaatccttt ttggatttcg
     9721 tcttgcgcaa tcgtaaattt taaaacacca tcattcgtgc cttttttttc aaatgttgca
     9781 gtcatttaat tccctccgag tttagaaatt cattttatac atcaaaaacg tttcatgcat
     9841 acctttaaat agtacactaa ccctttcaaa ttgtaaacat attgaatgga tagaatcctt
     9901 gtttttcata acatttttat ctttttcact gataaaatct aatggaagaa gatcatttgc
     9961 tgcaaaattt tttctctttt ttttaaaaaa tcttgttttt catcttctgt accttcatac
    10021 atcgtttttc ctgtacacgc ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg
    10081 tatgtatcat agacaggata aacaattgca gattgcaacg tgaattcttc ttttaacacg
    10141 gaactaagca gcgcatcttc attcccgtaa cgatctgaaa atacacataa ttgctggtaa
    10201 cttgcctgct gctgcggctt tggcagatct tttggtatga ctttgactaa cttttcttca
    10261 atcgtcaaat agaaaacagg atggcattcg ttcaattgcc taagtgtctc cagtaatttt
    10321 gcccttgcta aaatatggac ctctggcgat acaataagtt tactcgctgt ttggataaac
    10381 tcatgataag gaagctgttc gatctcatca atagacgcaa gctgacgtgc aggttccaac
    10441 gaaggaagtt cctccgattt attgtggagt gccttgatct tctgcctctc catttgtccg
    10501 gataattctt ctgcaaattg gattttttca atgacccgct gctcgaaagc ttcataaaaa
    10561 tctgtacttg ctatcaattt tctagcatat aaaaaatctt gtgtactgat taacaagtca
    10621 aaataaaatt cagcaaactc ctcattttga agatactgtt tttcatgcaa gagtgcttgc
    10681 ttcaatgctt cttttttctc gtccaactcg taaagacaaa aaacaagcag gctattagcc
    10741 tcaaagcttt ctttcaactc gtaagctcgt tgaaataatt cttttgcctg ctgatattgc
    10801 tggtttgcta gtgcttgttg tgccactcgc attaaattct catatttttc tggaaaatca
    10861 atcggcttca cgcagtgtcc tcctcttgct catattcagc tcatgttcaa tagtagcaaa
    10921 acaaaaaaag catgacaatt aaaaataact gaagcaccct ctaaaagcta gattttagat
    10981 ctgactttta gaaggtgctt tcaactgtta ctgcttttta ttgctcatca gctgttgact
    11041 tcctggttga acgctttgat cagattgatc gtaacaacac ttgtattagt gaaaaaatcg
    11101 cccagatgtt gctttttctg attaaagact gctggaagat accctaacat cagcaaagga
    11161 aattttagaa tgaaccggca agcaccttct ctaactaaaa cggttgacca gctaagttct
    11221 tcttcatcaa agctgatgac tcgaatacca aagatcattt tcccaatcgt ttggccatga
    11281 ttgaatttcg tcaataaaat aaaatacgct agataaataa gtaaggctgt gaatccgtag
    11341 agactcagat aagaatctga aaaattaagc caacctagat ttttggcaat accaagtgtt
    11401 aaacgcgtaa tagctccgat acacaaaaga tcgaccaaga aggcaaacat tcggataaaa
    11461 aaacctgcat agaaatactt aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc
    11521 gtatactttt tccatttacg ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt
    11581 tcaaaagagc tcagtacctt ttgggaaaag ttggagtcag gtcctacagc aggctgctgt
    11641 tgcttatctt tttcttgttc cttattttca tttgaaagct ctgacatgct tactcacctc
    11701 cgtaataata cattgctttt ggtgattccg ctgtccctag attctcaaga acggataaga
    11761 tctgactagt ctcagaaggc ttcaatccct ggaattctgc tagtttactt cctaaccatg
    11821 tactagcaaa cccagatggc gtggaagaat attctactag ttccgcatct tcaagctgtt
    11881 gttcctttcg tatagctttt aaagcatctt caggaaatcc caattcatcg actaaaccta
    11941 cttcttttgc ttggacacca tcataaatcc ttccatcagc aatttttttg acttcttctt
    12001 cagatttatt ccgtccctcg ctgacgattg ctacaaaacg actatatgcg ctgtcgatat
    12061 atgcttgaag caccgcttct tcttcttttg tctctggtcg cgttcctgaa cccatatctt
    12121 tcaacgctcc gcttttaact gttgtatctt ccacacccag tttttctaga agtcctgaat
    12181 aattcaatcc tgacatgatc acaccaattg agccggtcac tgtttcttct gttgcaaaga
    12241 ttttatctgc ttgagcagaa atatagtagc caccgctagc tgccatgttt ttcatgctga
    12301 catacattgg gatatcatgt tctttacgaa tcgtatccaa tttcttagcg atttccgcac
    12361 tttcgtagac accgccacca ggagaattta cttctaaaaa gatacctttg actgttggat
    12421 cttcttcaat cgccttcagt tgttccataa aatttttatg attgtaacct tcagaagcaa
    12481 ataaacctga agaccctccc gcagcaatcg ttccatctac tgataatttt acgatttttt
    12541 gcgtactgtc tccctcttcc aacacatttg gagctagttc ttcatcccca tataaccaac
    12601 tgttcaatcc ctcaatttga tcgttttcct gaggcttagt caaactagct gaaactaacg
    12661 aaaccacaaa taatcctgca gcaatcagaa ctgctgccca acgtctttta ttcattcttt
    12721 ctcctttcta atacaagttt tttatgacga tcacagtttc tgactgctca gaagtgtcga
    12781 accgctctgc tttttcttcc cgcgcatacc cggaaaacga ctcttttgtt aattcatctt
    12841 ctaccgtaaa ctcacgagct tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc
    12901 tatcccagcc agtatctaaa aatattttcg tatcatctga tacaccagct gatgcttcga
    12961 atttggacaa ttgttttttt aacattccca gtgtcatgat ctcaattttt gaatccattt
    13021 ttactcctcc tctatccgat tactttctat tgtaccgaac tcctgaaaaa gatgatagat
    13081 aaaaacccat tattttttga cataaaaagg acatttgaca aagaatttga tatataccaa
    13141 tttatttttt attctttttt tgttgacaag aacattcgca aaaggttatg attttaaatg
    13201 tttccagata aaaaacaaaa aggaggatag ttataccttt taggatagcg ccaggtctgt
    13261 gtaaacagat gacgaggaga gggtttatcg aaagattcgg cggatggccc tagggactgc
    13321 actctacaga aaatccacaa aaaatgtctg caaagataaa acaaaaggat tttcctgcag
    13381 ctggaagcat tcatacgaat tcattataga aagtaggatg ttttttatgt gcggaattgt
    13441 tggaatgatt ggattaaaaa atgtaacacc aggattgatt gacggtctag aaaaattaga
    13501 ataccgtggt tatgattcag ctggtatctt tgtctcagat ggcacaactg attatctggt
    13561 caaagcacaa ggcagaattc aaaatttgaa aaacaaaata aatattgata ctacaggaaa
    13621 tataggtatt ggtcatacac gatgggcaac tcacgggcaa ccttctgaag aaaacgccca
    13681 tccacatact tcccaaagtg gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt
    13741 tgaagaactt aaaattgctt atttagcaaa tgaccacttt attggtgaga ccgatactga
    13801 gattatcgct catctaattg aaactttcgc gaaggatact acaacgcaag aggcgttttt
    13861 aaaagctttg cgggtcatca aaggttctta cgctttcgct ttgattgatc ggacagcacc
    13921 tgatgttatt tacgtcgcta aaaataaaag tccactatta atcggactcg gcgatggatt
    13981 caatgtgatt gccagcgatg caatggctat gcttgctcat acgaaggaat tcgttgagat
    14041 tgaagatgaa gagatggtta ccgtcacatc agaaaaagtt attattcaaa attttgcagg
    14101 tgacatcgta gaacgttctt cattcgaagc acaagtagat gcttcagata tcgagaaagg
    14161 tacctatcct ttttatatgc tgaaagaaat cgacgaacag cctattatca tgcgtcgtat
    14221 cgctcaaaaa tacgtaacag aagacaatcg tgtctctctt gatcaaaagc tggtcgatac
    14281 attgtctgat agcgatcgaa tctatatcgt tgcttgcgga actagttacc atgcggggtt
    14341 agcaggtaaa cagactttgg aaaaattgac acagattcct gttgaggttc atttagccag
    14401 tgaatttggc tataacacac ctcttctttc aaaaaaacca ttctttattt tcttgagtca
    14461 aagtggcgaa acagcagaca gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc
    14521 ttcacttacg attacaaatg ttgctggttc tactctttcg cgggaagcat cctttacact
    14581 gctattgcat gctggacccg aaatcgcagt agcatcaacg aaagcttata ctgcacaaat
    14641 agctgtacta actttattag ccaaagctat cggtgataaa aaagaatttg aaacttctct
    14701 tgtattcgat atcactcatg aattgagttt agttgctaat gcgatggaat cggtcatcgc
    14761 ccaaaaagat tatttagaag agttggcaga agtatatctc agcgatacaa gaaatgcctt
    14821 ttacattgga cgtggtgctg actacaacgt ttctcttgaa gctgctttga aattgaaaga
    14881 aatcagttat atccaggcag aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc
    14941 tttgatcgaa gaaggaacac cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca
    15001 tactcgagga aacctgaaag aagtcgaaag ccgtggcgct aaaacactag ttattgtctc
    15061 agagggatta gaaaaggaaa gtgaccagct ggtgctacca actgtccacc cttatctaac
    15121 tactctaacc acagtcgtac cgactcaatt acttgcatat tacgcaacgc tattacgggg
    15181 attcgacgtt gacaaaccac gtaatttagc gaaatcagtg acagtagaat aataaaagaa
    15241 caaggaccag tgtcagacat ctgacactgg tccttgttct tttatttttt atgaaaatat
    15301 ctcttaatcc agtccgattt cttctttgac tactgccgcg attttatcga catagtaatt
    15361 aactttctca tcagtaggcg cttctgccat cactcgaagt aatggttctg taccggaagg
    15421 acgaactagg attcttcctt ctgatcccat ttcagcttct gcctcttcaa tcactttttt
    15481 gattgccgga acatccatcg caccgttttt atcacttacg cggatattca ctaatttttg
    15541 cggatagatt gttacctctg ctgcaagctc tgataatttc ttacctgtct gtttcatgat
    15601 attcagtaac tgaattcctg aaagcatccc atctccagtt gtgttaaaat ctaagaaaat
    15661 catgtgccca gactgctcgc cgccaaagtt ataatcgttt ttgcgcattt cttctacaac
    15721 gtaacgatca ccaacttgtg taacgacatc tttcaaaccg attccttcca ccgctttgtg
    15781 gaatcctaag ttactcatga cagtcgtaac gatcgtatct tttttcaatc gatttttttc
    15841 tgccaagtat ttagcacaga taaacatgat tttatcaccg tcgacaatgt ttccaagttc
    15901 atccaccgca ataatacgat cgccatcgcc atcgaaagct agtccagcat ctgctccttt
    15961 ttcaacgacc atctcagcta aacgttctgg atgggtagag cctacgccgt cattgatatt
    16021 taacccgtct ggatttgtgc ccattgtata aaaatctgtt tcaagatcgg caaacaaacg
    16081 attaacactt gttgaagttg ctccgtttgc tgcatcgaca caaatggtca aaccggaaag
    16141 gtcaccgttg atcgtttgta caaggaattg agaatatttc aataagcctt ctggaaattc
    16201 ttctacagtt cccaatcctt cagctgatgg acgaggcaat tcatccactt cagcatctaa
    16261 taatgcttca atttctagct cttggtcatc tactagttta aaaccatctg caccgaaaaa
    16321 tttgattcca ttatcttgtg ctggattatg agaagcagaa atcatgaccc ctgcgcttgc
    16381 tttttgcaaa cgtgtcagat aagcaacacc tggtgtagag atcacaccta gctggaacac
    16441 ttcgataccc accgataaaa gtcctgaaat cagcgcttct tctaacattt gacctgagat
    16501 tcttgtatcc cgccctacta acacgcgtgg gcgttttcct tcttcttcat gctggcttag
    16561 gacataacct ccgcaccgtc ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc
    16621 ttcaccgcgt acgccgtctg ttccaaaata tttacccata gttgtaaaat tactccttta
    16681 acttaatttt ctgaggaatt tgttctgctc atttgtgttg attcgcttgt ttctgatgtt
    16741 tcttctgtac ttgtggtcga gctgcttgtc ccagattccg aactggaaga aatggttgtt
    16801 gtcgatggtt ccgttcctga atcagcagat gaaatacttc tactcgtact tgtattttct
    16861 tgtgtccgac tgctcgtcga agcagagcta ctgcttgacg tttgcaacac gggtgtaatc
    16921 tccacactaa ctgattcggg cttgatactt aacccctctt ctactggtat ttcaatttta
    16981 cgagttgttc gatgattgat acctgagaca ttgatcggta cagcaatact gtcaccgatc
    17041 gatgcaagca aatcgctaga gccgcttact tctgcttcca gttttgaaaa actgaaatca
    17101 tagctttcta ccccggcagc tggtgttcct tgctgtatcc cataaagtcg taccgacttg
    17161 cttggcttga tcgaatcagc tctcactgtt acttgcactg gatcagagac gatacttaat
    17221 acttcccctg ctgcattcag cgcctgtatt gggacctcac tattaatccc atcatctgtg
    17281 accatcgaag cgtccacatc agctactact cgtttgattt ctgccaatgt tttgtcacct
    17341 gtagtgatag agactttttt aggactaacg gaagtatcat caagttcata tccagatggt
    17401 gtgaacgttg aaggaatcaa cgtttccaca tcgaaatctt ttattacctt tttctcaacc
    17461 gttactgtaa tagtcgatgg ttcgatcttt gctgtgacag cactgctcaa attttgaatt
    17521 tttagcggaa cttcatgtgt tccttcgccc agcttagtga tatctgctgt gacgcggaac
    17581 atccgtgtat cttcgtttgc ctctgcattc aactgaactc ggtttgcact ggaaagtttg
    17641 acagtcactg cattttcata tccatggata tagtagcgct ttgaatcata agaaatcttg
    17701 atcggcaccc ccgtaactgt ttgattatac gcttcatttc cagaaagcgt atttggaacg
    17761 gactggccat ttgcattgaa gtacagcgca atactgaaca gcaatgcaat aaaactgtaa
    17821 agaatgcttg tccgtttctc ttttgagatc atttgcgatc ccctccctta gtcataccgt
    17881 ctaggaaaga ttgaatcacg tttttcttgt tttttgtttt ctcttcagca attagttctc
    17941 tacgcaagat tgccaaatat tcttgctggg ataagccagc catcagttca ttatctaacg
    18001 tgatgctgac gtccccggtt tcttcagaga caacgatggt aatcgcgtca ctgacttcgc
    18061 ttataccgac agctgcccgg tgtcgagtcc cgaattcttt agggatcaac atactttctg
    18121 atagaggaag ataggcgctt gctactgcga tctttccttc ttttacaata actgcaccgt
    18181 catgcagcgg tgtattagga ataaagatat tgatcagcag ctccccagtg atatctgcat
    18241 ctaaagcaat ccctgtctca atatactcat ctaatccagt atgacgttcg atagtgatca
    18301 acgcaccgat ttttcgcttg gacatatatt ggatggcttt gtcaaaagac aagatcatct
    18361 tctcatcttc ctgctgttcg ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt
    18421 ccagtcctct ccggatttcc ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa
    18481 tgacttggtt catcagccaa gacaaggtgt gcagaccaat gatttccgct acgattcgga
    18541 taacaataaa aatagctaca cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt
    18601 ggatcagttt gtatacaaga taccagacaa ccagaatatc aagtatgttc accaaaaaat
    18661 tcagagagaa caagtcaccg gaaaacattt gctgccaata gtccaaatta aaaagttccc
    18721 cgatttgaaa tgacataaaa agcctctctt ctcttttgag atttcctctc tagtatatca
    18781 tatgttttaa ttttagaggc tgatatgctc gtatttttct tgagaaaaac gcataatttt
    18841 caagaaaatt gcaagtatgc gttttatttt tctctgcaat aaatattttt aaacttcaga
    18901 gaattattca ttatattttt tctgttattt ttcctttttt tcttttgtag cttgttccat
    18961 ttttttacgt cgttctgctc tggcagctct tctttcttct gcttcaaggt ccgtcgtttt
    19021 ttcttttcta gtcttaacgg gtacatcgat agattcctct ggttcaagtc gtttcttgat
    19081 tttttctaat tctcccgtca gtgcagcata gttgtaaaaa cgtgttttag cgtcttggat
    19141 agtcttttca aataaagctt ttgcaagatt tggatttgcg ttctctagcg aggaaaagcg
    19201 aacttgtttc ctcatgaaat caggcatagc agagaaatca ggttttttgt agtccaaagt
    19261 cattgggttc ttgttttttt cgaccattgc agggttatat cgatataatg accaatagcc
    19321 agaagaaaca gcttcttttg cttctttaag cgattctttc attccaccaa gcaaaccatg
    19381 tgtgatacaa ggcgtatagg caatgattaa agaaggtcca ggatactttt ctgcttcttc
    19441 aaaagccttt atcgtctgca tttgattggc acctgaagca atttgcgcca cataaacatt
    19501 tccatatgtc atcgccatca ttcctagatc tttctttgaa gcatactttc cactagccga
    19561 aaatttagcg attgccgaag ctggtgttgc ttttgaggtt tgaccgccag tattggaata
    19621 gacttcgtta tccaaaacaa gaatattcac gtctgctccg ctggcaagta catgatcgat
    19681 cccgccaaaa ccaatgtcgt aagcccatcc gtctccacca atcatccatt ggctgttttt
    19741 tacgaataga tcctttctct tgtatatatc ctccagttca gggacaattg atcgttcgct
    19801 ttcgatcgca gcttgcagtt ttgctgctct ttgctgcgtt ccttcgctct caaacatgtg
    19861 taccaaccag tcttccatca attctcgtag ttcttctgat acttttggca atacttcttt
    19921 gattgaactt gccaaagcct cgcgacgagt ctgtgccgct agaagcatac cataaccaaa
    19981 ttcagcatta tcttccaata aagaatttga ccatgcaggc ccttgaccac attcatttgt
    20041 cgtataagga gaagcaggag aagcaccgcc ccaaatagaa gaacagcctg tcgcattcgc
    20101 gatcatcatt cgatcaccga acatttgtgt caataatttt acataaggtg tttcgccaca
    20161 gcctgagcaa gcgcccgaga attcaagaag cggttgattg aattgtgtag acaagacagt
    20221 attctttcct ttgactggat ttgctttttg tcttaatgtc attgcaaaag cccaattcac
    20281 agcttgttct ttctgttctt cataaggctt catcactaaa gctttttctt ttgcgggaca
    20341 agcctctaca cacagaccac aacctgtaca gtcttccact gaaacttgga tacggtattt
    20401 tacgccgtct gcgcctcgca tttctctcac gataaatcct tctggcgctt cttccatttc
    20461 ctcttcatct gctaaaaatg gacggatggc agcatgagga caaacaaagg cacattcatt
    20521 acacatcgta cagcgatcac taatccactc aggtacttct aaagcgacgc cccgcttttc
    20581 catagaagcc gttcctaaag gcatttcgcc agttgtcatt ccatttgcta caagtgtacc
    20641 aacagagagt tgatttcctt cctgtgcatt cactggttca agtatttctt gtacataacc
    20701 agtgttcctt ttattgattt tttcaggcat ctccaacgtt ctccaatggt ctgggatcgc
    20761 gacttgatga agcagttcgg tcgtccggtc gattgcttgt atgttttttt ctacgatagc
    20821 cattgattta tgtccatagc ttttcagggc ttcttctttt agcagcggaa tcacttcatc
    20881 aaaaggaatg atgtcagcta atttaaaaaa tgccgtttcc attgccgtat tgatccggcg
    20941 tcccaaaccg acttcttggg ccaatctcat ggcattgatc gtatagaatc taatgttatt
    21001 ttcagccaag taacgtttta attttaaggg cagatgcgtt tctaattgtt cgtcgctcca
    21061 aagagtattc aataaaaaaa tgccacctgg cttcaatcct ttgaccaagt tatagctatg
    21121 caggtaagct ggtgcgtgac aggcaacaag gtccgcatgc tcgacaagat acgcagaacg
    21181 tatcggcgta tctccaaaac gaagatgaga gacagtcaga ccaccagatt ttttcgaatc
    21241 ataataaaaa tagccttgcg catatttatc ggtgtgatct ccaataatct tgatagctga
    21301 tttatttgct ccgactgttc cgtcagatcc aaacccccaa aacttggcct ggaatgtttg
    21361 cggttctgtc aaatccagac tttcctttgg ttccaaagat tgataagtca catcatcaac
    21421 gatacctatg gtgaaacgtt ttttgcgtat gcttgggtct ttttgcaatt catcaaaaac
    21481 tgctgagatt tggtcaggag tcacatcttt agaacccaat ccgtagcgac caccaataac
    21541 agccgggcga agttcagaat catataacgc actttgaacg tctaataaga gaggttctcc
    21601 acctgcccct ggttctttac ttcgatcaag aacagcaatt gtttttactg tatgaggcaa
    21661 cttttgcacg aatgtttcca ctggaaaagg acgataaaga tggatattca aaaaacctgt
    21721 tttccgtccg tttgcattca agtaatcaat cgtttgttca atcgtctgtg caactgatcc
    21781 catagaaaca atgacttctt ccgcatcgtc tgctccgtaa taagtcacca agtcataaga
    21841 agttcctcgt aatcggttga tctctttcat atatttttga acgatttcag gtaatttatc
    21901 ataatagggg ttgatcgttt cccgttgttg aaaatggatg tctggattct ggttcgtacc
    21961 actaacagtc ggatgattgg gattcatccc tcgtttacga aatgaagcta atttctcctg
    22021 attgatcaga ggagctaatt cttcatattc caaaacttcg attttttgaa tctcgtgact
    22081 cgtacggaat ccatcaaaga aattcaaaaa aggtacttct ccttcaatag aagcaagatg
    22141 ggcaacaggt gctaaatcca tcacttcttg tacactgctt tcagctaaca tagcaaatcc
    22201 tgtttgtctt gcagccatca cgtctccgtg atctccgaaa atattcaacg catttgtcgt
    22261 cactgcccga ctagcaacat ggaaaacact tggcagtagt tccccagcaa tcttgtacat
    22321 attcggtatc atcaaaagca gcccttgaga agcggtatat gtagttgtca gtgcaccagt
    22381 tttcaatgat ccatggacca caccagctgc tcctgcttcg gattgcattt ctactacttt
    22441 tactggttga ccgaatacat tttcttttcc tgctgctgac cattgatcta ctaattcagc
    22501 catggtcgaa ctgggtgtta ttggatatat tgcagcaatc tcggtaaatg cataagaaat
    22561 atatgcggct gccgtattgc catccattgt tttcattttt cgcatgattc atcatccttg
    22621 ttcatattct ctcttattat ctgttccatt ataacgtaaa acattccatt ttttctttta
    22681 tttataaaaa tatttcacat atgtctattt gaattggtca tctaattttt agacaaacag
    22741 tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag aggtgtttta
    22801 acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga atcctttatg
    22861 gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cctatcttgt ccgtattgcc
    22921 gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc gggcaattgc
    22981 agcgagtgat caaattgttc gataaagaaa aagaaagctt gcagcgagga aacgtgatgc
    23041 accggtatct cacaatcagc gatggtcaaa aagcgatcaa agagatcaaa caaatattcg
    23101 atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt gcagtagata
    23161 aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc aacgaagctg
    23221 agcaagcaca tattcgattt gttccgactg tcatcttggg taaagaaatc tttgatgaat
    23281 ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat aaggagcgta
    23341 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cctataaacg gcggaagcag
    23401 aagcaactcc ttcttatttc ttggagttaa acgcttctgt cccagcctcc ttattccgaa
    23461 taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga caagctgagc
    23521 aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta actaattctt
    23581 aaattttcat acaaaatttc tcaaattccc ttaacgagta atatttatgt acggtgattc
    23641 acactagtat cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt
    23701 tgcttttatg tatgctgtta gttgattaga tggtcgtttg ttgagctagg tcgtttattt
    23761 taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca
    23821 caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca actcctaact
    23881 cctctcattt aatagttgct ctctttttta ttcagataca tttttgctga tcacagccta
    23941 gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat
    24001 gaaatggatt aatagatttc gattattcaa agcgattttc tattggtgga ttgtgttccc
    24061 aactaacaag acggattcca aagtcattat ataactcttc aagagttaac cctttcctgt
    24121 tcccaataga tgtatctctc ccacgctcga tataaaatat ggagtttcga atgccaagac
    24181 ctggcaaatc tttccttgtt acagttacac tgctactaac ttccccatat aaaatccagc
    24241 tcctccaaaa tgataatata tttttcgacc atcatctaac tcatgtaaca ataattgttg
    24301 tgccctcttg tcactcgcca aatgcaaacg ccacgtgcga taccatgcca tgctacctgc
    24361 atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa tcctttcccc
    24421 atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatcgcag gtcttggtgg
    24481 cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa acacagctat
    24541 aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta ttcgcaaatc
    24601 acctctacat tctcacatac tagttcgtaa tactccttta atatcaaaat atctactgat
    24661 cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa tcatcagctt
    24721 ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa tcaccaaaat
    24781 tgttaacaat cggttcagat atttcccaat tcactagcct tatattatat tgatttaaca
    24841 attcttcttc atttattgta ttttgatcaa gcggaccaaa ttgttctaat aattctttgt
    24901 catgtaaaat atcccttcta gtaatgcctc tttccctttc aatgaaagaa gcactaggaa
    24961 aagtatgatt ataaccattc aacctatctc ccatgtaata attagcaggt cctggaaaat
    25021 aaaatatttg cttattatca ctaacttcta acagcaacac taaatcttct ccgctggcta
    25081 aacgacttgt ccatctacga tatctcccta tgccttcgtt cattccaaat ccatcaaatt
    25141 caactattaa cgtgtcttct atcacatgta attcttcatt caattcatac tcaagtctaa
    25201 aaggaaattc tccatatgta atctctggca atggtggtgc tggagttctc caaataaata
    25261 caataactgt gaatagaaaa aatccaacga tcgatataaa tgttatgaga cctgctcgaa
    25321 gtaaaacccg atataataaa aatgctgtat ttcgctttgc tatatctaga aaaataaaaa
    25381 gtactgcaat cataatgata gtaaaagttg taaaaaaacg catttttgcc ctccctttat
    25441 aaacatcgat tctcaaattc taaaccaaat cttggtagtt caagttcagg tataacgtat
    25501 tccaaccata catctcaatt actatttatg aacttatgat tatcactgaa taaatcactc
    25561 catccccttg ttaaaacttc tacattaatt acacctggtt gttttcgact agctaatcca
    25621 atgaacttat caaccatcct tatccaccaa ccacgagaat cttgaccaga agtaagaaga
    25681 gcagttctca aggatgatcg agcagttcct tccagttttt taattaatca ggccagttat
    25741 agataaatac ttgatcttat ctatatcagg acttctatat taagcaaaaa tttatgtaca
    25801 aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt aaaaacaata
    25861 gaagcctttc caaattatgt tactccagta aagatggaat tatagtttga cggaagaaaa
    25921 gcaagtataa cttttaaaaa aaactgatac attcgcatct cctgcttcat gtatcagttt
    25981 tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt attttgcgaa
    26041 ttcggtgatt aattcccgat tttgatatcg cacctgaggg aatcatttcc cctaattctt
    26101 tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt ttttcaggaa
    26161 gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt ttagaagccg
    26221 catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg accgaatttc
    26281 tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt gcaccaatca
    26341 atgttaagaa atcagcaatt ttttctgctc cttttaaata agaaatatag ccattgcgcc
    26401 gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg tcttggttat
    26461 gctgttcata aatagaaaat atttccaaat gataacggct cgtttccgga ttattgactg
    26521 aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa tttcccataa
    26581 tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga tccatcaaca
    26641 catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt aatttcattt
    26701 ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga gaatagatac
    26761 gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg ttcacaatac
    26821 ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt tctcgatgaa
    26881 cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta ttctcctcct
    26941 ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac aattcttcta
    27001 ctactttgtc tccatcgtgg aagacaccac catctttcaa ttccaaaaag tcggtggaaa
    27061 tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt actagatact
    27121 catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg accaatactg
    27181 tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg gcatctgtga
    27241 aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt tcagcagttg
    27301 tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata ctggtgaata
    27361 aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg acttttcgag
    27421 ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca atggttttac
    27481 ggtcgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaag acagcatgca
    27541 aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc atttttgcta
    27601 ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca atgatcagat
    27661 taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat tggaaaatat
    27721 cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag tcaccaggag
    27781 gagccatagc gatcgattct ctgattgcac cgctgcttcc cccatcatct gcaacagtca
    27841 ctactgctgt gatatctgct ccttgatttc gcaaactttt caggataact ggcagaccag
    27901 tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc attttcacga
    27961 ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga ctttatagtt
    28021 tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt gaccgcctgt
    28081 acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag gcaatacagt
    28141 ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg acatcacata
    28201 atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat aatgagggtt
    28261 gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt atttaaaacc
    28321 aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg actcattgat
    28381 tttttcacgt aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg aagcttgggc
    28441 tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca aaccgtccat
    28501 cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt cgtctgaagc
    28561 atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga tcagcatgtt
    28621 ttggatttct tcaaagaagg aacgagaacg taaatccact cctaaagcca tttttgtgac
    28681 tttgcctgat tctttgatta attcccagaa ttttggaatc aatcgaggcg gcatattatc
    28741 gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc ctgcaccgct
    28801 catacctgtt atgatgacta attgcaaatt atctgtcata attacgaact cctctcacat
    28861 tgtgcatagt aaaattatat cattccgggc gtatcattac cagttttctt tttcttttac
    28921 aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt tttttacttt
    28981 tccgatgctg tatccaatgc cttgcttctt tcagctagcg ctcgttcaat cgtccgatct
    29041 gctaaaatag attgggcatc gatcactgga taatggttat cttcagcaaa ttcatgcatc
    29101 aatgacaatt ctgtgcaacc taagataact ttttcacagt ttaggcgctc taccgcttct
    29161 tctaagattt cgtaatatag ctcttgattc aaataatccg attccttgat ctcatgatag
    29221 atcagataat tgattttttc ttgaagaaca gtatcaggaa tcactgtttc gaatccaaga
    29281 tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc tagaatcgct
    29341 actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg catatgaagg
    29401 ataggaatat ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt gttacaagtc
    29461 aacacaatga agttcggctg caataaattt tgtttctcaa tatcatccag cagaaacggc
    29521 attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac tgttgcatga
    29581 ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg gtggttgatc
    29641 aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact gaaaaaattc
    29701 tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat atagttatgg
    29761 aacatatatt tcatcaacaa ccagcgcttg attgaccggt ctttttcata aaacacagta
    29821 gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc attttcccga
    29881 gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt atctgattga
    29941 ttcgttccat aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc tgtgacgaaa
    30001 cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg tctcaaattg
    30061 atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa attggcaaag
    30121 cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt ttcgttataa
    30181 tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc taacagtgga
    30241 tgaccaagac acatcatccg aacttgatga tcttcatcta catacgcatt tagcacacgc
    30301 atattactat catcacccgg gataaaatct tgtacaatca tttcactttt ataaccggct
    30361 tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat aaaagctttt
    30421 tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt caaagcaact
    30481 gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc ttctctgacg
    30541 atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa gcttacttta
    30601 ttgatcagtc tttcaaataa agaataatca atatatggac agataaaata ttctgacagc
    30661 tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat caaaagatat
    30721 tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc gataaacaca
    30781 gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta gcgggtaggg
    30841 gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt aaaagaacgt
    30901 gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc ttcattttca
    30961 atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt tccaactaca
    31021 caaggaatat cataccataa agaaaaaagc aatcaaagga aactcctccg attgctcaat
    31081 tcttttttga acggattgaa cttataggaa gtttttgtct ataccaatca gtgtagaaag
    31141 aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa attcattttt
    31201 ttatgttttt ttaactatct cagctatcta tcctctttat accattattt ttttcaaata
    31261 gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag tcgcaaggat
    31321 cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg ctgttttgat
    31381 tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta atctttgtaa
    31441 tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt catctagtat
    31501 atagaaattc tttccattag agattttgtg caattcactg gcaagtttca tccgttgtgc
    31561 ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta agccaacatc
    31621 aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt ctacagcatc
    31681 ttctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca cttctaatgt
    31741 ttcagagtta tatcgtttgc catgacatac ttcacaagga acatagacat caggcaaaaa
    31801 gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac cgccttttac
    31861 gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg tttgagcaaa
    31921 taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc tcggcgtccg
    31981 gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc cggaaattgt
    32041 cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg atttctttaa
    32101 gatactattg accaatgtac tctttccaga accagacaca cctgtgactg ctacaaattc
    32161 tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac cagtgatctt
    32221 gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt ttttccctga
    32281 aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag tgcctgcagc
    32341 cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcagat aatctgctgc
    32401 ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat cacgcatttt
    32461 ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg atggttcatc
    32521 taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc tgattcgctg
    32581 cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt ctagtcccac
    32641 attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg caattacttt
    32701 ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa tagacaactc
    32761 atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg gattcaatcg
    32821 ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt gctcacgcgt
    32881 gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc cttcgaaagt
    32941 cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga aaagatcatc
    33001 tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg gtgtatctaa
    33061 atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact gagagctgat
    33121 tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg gaatcactaa
    33181 atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag ctccaaaagg
    33241 cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc aataaggaca
    33301 ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca gagcatagcc
    33361 gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa tcccttcttt
    33421 cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct ccagctcagg
    33481 cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc cctctttttg
    33541 gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg gtgccaacac
    33601 ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat ctactgattg
    33661 actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg caaacaacaa
    33721 gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat ttttacttgt
    33781 tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta cgtccggctt
    33841 atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt atctccgctg
    33901 cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg acaaacctgt
    33961 aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt tatgggcacg
    34021 tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg aagcttttaa
    34081 ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt ttgccgcatc
    34141 tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg tcatttcttc
    34201 gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa tagaaatcaa
    34261 atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat tgtacttctg
    34321 ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat ctgttacttt
    34381 gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa tcgtttggac
    34441 taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag caacaaggga
    34501 aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca catcaaattc
    34561 acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat cactatgcaa
    34621 atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg ccattttttt
    34681 cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta tttcgcctac
    34741 tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa gcagccctgt
    34801 cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg gtcctggcgt
    34861 cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc gaagtggccg
    34921 attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt ttctcgctcg
    34981 gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt ccgccacgat
    35041 caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt ctttacgtcc
    35101 gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta acatcatttc
    35161 aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt tttcatttcg
    35221 caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat gctccatatg
    35281 ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg tttctcctaa
    35341 aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc caaaaaactc
    35401 cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat caccgcgcac
    35461 tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac tgactaagtc
    35521 acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta cttgtttttg
    35581 gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga cgatcacatc
    35641 atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct catcatttac
    35701 acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag gctgataata
    35761 atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct tgaattcccc
    35821 gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt tattcacttt
    35881 ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta ataagatttg
    35941 tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag gctgatcacc
    36001 agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct caatcattct
    36061 tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa aaggaaagca
    36121 aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac ttttttatct
    36181 tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt tttcattttt
    36241 tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa atattggaaa
    36301 tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt cttaatgaca
    36361 ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac aacggcagag
    36421 tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata cgtaattgcc
    36481 agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta cgaaggcatc
    36541 gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg aatggactac
    36601 cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg aatgattgcc
    36661 ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc ttactttgat
    36721 agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta tgaagatttg
    36781 aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt aagcgaccat
    36841 gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt gtacgacggg
    36901 ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg ttatgcgatc
    36961 aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa atatgcattt
    37021 gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg attgaaagaa
    37081 ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga tggttcagaa
    37141 acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat ccaaaacaac
    37201 tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat ctcctttgct
    37261 ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat acgcgctctt
    37321 cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat ggttcttgcg
    37381 ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga cttccttgct
    37441 ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt tcgaggcggg
    37501 atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct ttcttataat
    37561 cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc atcattcgtc
    37621 aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg tgtagtcgaa
    37681 ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta tgtttacttg
    37741 atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc aaaagtttta
    37801 gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag tcaaaaaata
    37861 cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag atgtcgtctg
    37921 tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga atcgtttaga
    37981 agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata aaagcacaga
    38041 tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc tattccctca
    38101 tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag aatctagaga
    38161 agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag ataaaaaaga
    38221 tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg ctcgtgctct
    38281 agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg atcctgaaat
    38341 ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga cgatggttat
    38401 cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt tcattgacga
    38461 cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc aaaatgaacg
    38521 gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa tgcacggtcg
    38581 ccatgaaggt accgtgtatt tttatcttct tattttctca cctaatcgta tcagccgcat
    38641 cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat aagaaagtac
    38701 catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta attgagcttg
    38761 atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag caattgtgtc
    38821 aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat tacggacagg
    38881 ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt tccagagcct
    38941 cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat cagttacagc
    39001 taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa tctttccttc
    39061 atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag atccgctttt
    39121 ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa cgaacattat
    39181 atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc aagaagactt
    39241 tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa ttatttattc
    39301 ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt tataccccta
    39361 gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa acatgacaac
    39421 tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt aatgacttag
    39481 cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt aaacttatcg
    39541 aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc gaatgcacgt
    39601 aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac tttgacatgt
    39661 tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa acggaagttc
    39721 gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac agttggtact
    39781 tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata gtaaatttca
    39841 gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata caaatcacca
    39901 aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg ttgtccttct
    39961 tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg tcctgagcca
    40021 tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac atcacaagtt
    40081 tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt acctgcacca
    40141 tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc accacaagta
    40201 tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat cgcttcttca
    40261 aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt tggatcgact
    40321 gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc gaagccgcca
    40381 ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc gtaatttgga
    40441 tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc acttaaaatc
    40501 tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt attgatgtca
    40561 ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc tgatgctcct
    40621 tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt tcctcctatt
    40681 ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa acaaaaaccg
    40741 atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc ttctctcgaa
    40801 attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc atcaacgaca
    40861 tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg tgccgcttgt
    40921 tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg tttagttttc
    40981 atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc tgtttctgct
    41041 tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt gtccacagag
    41101 aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc tttatctgct
    41161 tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt tagacctgaa
    41221 gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc agatacattt
    41281 acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc acgaggagct
    41341 gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc cattgggcgt
    41401 tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt tgagaatact
    41461 tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa cactccaccc
    41521 attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc tttcacatca
    41581 ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc agggttaact
    41641 gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc agggatacga
    41701 gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc tgcatctttt
    41761 agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa ttcatcaaat
    41821 tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc tgtgataaat
    41881 ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt ttcagcagcg
    41941 tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt ttcttttttg
    42001 aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc tagatggttg
    42061 tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat agatacgtca
    42121 aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt tttatctaaa
    42181 ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc tagaccagcg
    42241 atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc tggcacagtg
    42301 ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt gatatattga
    42361 aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc tactttgtaa
    42421 cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt cacagcttga
    42481 cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac agaaggtgtt
    42541 gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa tactgctact
    42601 gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa cgtctccttc
    42661 tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg accatggttg
    42721 gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca atcgtatcgg
    42781 ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta ggatcaaagg
    42841 cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta cgcaaacttt
    42901 ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac acttctgtgg
    42961 ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa tcttgtgaac
    43021 ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt gcgatttcag
    43081 cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat ttcgctgttt
    43141 cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg acatcgattt
    43201 ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt tctttcacga
    43261 tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag aaagagagaa
    43321 cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca agcgaacggt
    43381 agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga ccgaacatcc
    43441 gagaataagg catactagta ggccctaaca aggctattgt acctcttcca tgaccatcta
    43501 tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct aattcagcac
    43561 caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt ccgtctgagt
    43621 ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc tcataatcca
    43681 agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca agaacggtgt
    43741 caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc atcggaatct
    43801 ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg tcgtttacaa
    43861 tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg aacacttggc
    43921 tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta ttcaagggta
    43981 cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa aaagtcgtat
    44041 agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg atttcatgaa
    44101 attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg cgagcaggtt
    44161 ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc ccagaagaag
    44221 agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg cgaatcgttg
    44281 ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc acgccactac
    44341 tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt aacatcttcc
    44401 tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat atagcaagaa
    44461 gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca agagtgctaa
    44521 ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt tattgtattt
    44581 ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact atttttcaaa
    44641 caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca aataaacacg
    44701 gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat caccgtacac
    44761 cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc ctgttttttt
    44821 tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt tttcttcttc
    44881 caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg gtcctttgtt
    44941 gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa aatagtggtc
    45001 attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa agttacttat
    45061 ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa acattctggt
    45121 ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca tgaacattgt
    45181 tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag ccaaagcttt
    45241 ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa tcaaatcgat
    45301 gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca catcttctgc
    45361 ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca ctcccatgga
    45421 taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca aatcacctgg
    45481 attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc tagcacctga
    45541 taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc ctacgtaaag
    45601 tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat tcagcagcat
    45661 ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat cacagtaata
    45721 gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt ctgcgggatt
    45781 tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc tgttttcgac
    45841 cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt tagaatcaag
    45901 gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg cttttacagc
    45961 ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat ctgcaaaaat
    46021 atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc tttgcttaca
    46081 acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa aatccaagaa
    46141 tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata tatctctagg
    46201 gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa ttactttttg
    46261 ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt ttgtcatcca
    46321 tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt tttatttagt
    46381 tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa ttgttgtttt
    46441 cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc cttgttatag
    46501 atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga ggaatatgcc
    46561 gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg gcaaaacaaa
    46621 actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa aatcgattac
    46681 ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg caattcatgg
    46741 gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac agacgtacca
    46801 gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt gcaaggttac
    46861 aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca gaataaagaa
    46921 gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa tggaacagct
    46981 cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga agaaataaga
    47041 gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa gtacggagat
    47101 cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg acaagtaatt
    47161 ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata cactctattt
    47221 atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata ctggtattaa
    47281 aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt ggtctcttta
    47341 ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc gaccacagac
    47401 aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa atcagcttct
    47461 actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac ttcttctaca
    47521 tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac ctcctcacag
    47581 tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga gtacgcacgt
    47641 gtttggctag ctattagagg taatactttt agagatacat cgggtgattt cgaattacat
    47701 gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag tgcagtctat
    47761 ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt agtgtattca
    47821 agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca aatgcctgca
    47881 gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat tgataatgca
    47941 tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat cgatgtgaca
    48001 gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag cattttgtca
    48061 gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc caaggcagga
    48121 aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta ccgctacttc
    48181 tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat caatatcata
    48241 caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca ttttataatc
    48301 taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat caaaccagcc
    48361 tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct cacagttttc
    48421 tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt ggacatctcc
    48481 ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata atcgtccctt
    48541 catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga gatggaaaac
    48601 agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag attgctgtag
    48661 aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg tttcatccaa
    48721 gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc ctgtttttaa
    48781 tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt ttggttgtag
    48841 agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc aagtcggaaa
    48901 gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc gttttcttgc
    48961 tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta cttggaaaga
    49021 tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct ctgatacgcc
    49081 tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat aacttatcat
    49141 gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt cgtttgccgg
    49201 catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa ccgtttggat
    49261 gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg ataagagctc
    49321 ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag cgccctgctt
    49381 ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt taccgacgat
    49441 cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata cgtagttatt
    49501 atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc cttgttcttt
    49561 ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca actcaaaata
    49621 tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc ttttctattt
    49681 tattatttga gacatcttat catataaata tagcggttac aacattactt gtgttttgtt
    49741 cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact taatggaggc
    49801 gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa gaacatcaac
    49861 aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta gacacaactg
    49921 taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca tttagttttg
    49981 ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt gaaaaaaata
    50041 tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg aaaagtgtaa
    50101 tcaactggga attgattgaa caattgattt catttaatat tgaagataaa aaaggtcatg
    50161 ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg aaactattca
    50221 tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt aaacacttaa
    50281 ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa aatcactgat
    50341 tgaatggctt tgaattttct acttagtaaa attatttttt cagcaatttt cctgcaaaac
    50401 ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac tgcccattat
    50461 tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt tagagaaata
    50521 aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg ttctttctac
    50581 atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag gagggtgaac
    50641 attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta ctacacgttc
    50701 tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg ggcaatggaa
    50761 attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg aagcagcaaa
    50821 agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc ttcaatatcg
    50881 tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata acgatgttta
    50941 cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc atgcggagat
    51001 actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca atgaaaaatt
    51061 attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct agaaattcca
    51121 gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg ttcaaacaaa
    51181 tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc gtgaccatgc
    51241 ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt ggtgtcttat
    51301 tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc tgtgtaatga
    51361 tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat gctgacacac
    51421 taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat gcggttggtt
    51481 tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc ggaaagaaaa
    51541 tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa taaccaatac
    51601 ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca ggatcatcac
    51661 tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct ccaatgattt
    51721 tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag gttccgacta
    51781 actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg aaagatgtat
    51841 actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc aaaaatgtag
    51901 tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca agcaccgctc
    51961 caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc aaaatcagtg
    52021 gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt tttattcacc
    52081 cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct gcaaatagtg
    52141 taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc gccgctattt
    52201 cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct ttttgttcag
    52261 gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct atactattga
    52321 ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat tttaccccgc
    52381 cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga acagaagaca
    52441 tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc gctttcgttc
    52501 cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca aggctaagtt
    52561 catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc agtgtcccaa
    52621 aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg acatctgctt
    52681 gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt tctgctcctt
    52741 ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat cgttgagcaa
    52801 tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt tctaattcct
    52861 tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata acttttattt
    52921 ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata cgctctagca
    52981 atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt tgtgacagaa
    53041 gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga aaaaccattt
    53101 atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg gcttatttat
    53161 cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc ataatgtttt
    53221 attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt tcaatcaatt
    53281 cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt accgcgacat
    53341 gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc agctcccgtc
    53401 cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat gtttccccaa
    53461 tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc tttactttga
    53521 catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca actacccctt
    53581 ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg ctgtctagta
    53641 atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta gaaactgtga
    53701 cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca atgtcttttg
    53761 gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct acgatgtatt
    53821 gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca acttcatata
    53881 aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta gtcaaatatt
    53941 tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg aaagtcatca
    54001 atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg actttctggt
    54061 gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct tctggaattt
    54121 gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc tcatttctca
    54181 atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa atactgacaa
    54241 cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc gcaaatacag
    54301 tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta atatctactc
    54361 gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt gtcccatttt
    54421 tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata ccggcacttg
    54481 ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct gtatctacag
    54541 ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa gaaatggttc
    54601 ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc tgagcctttc
    54661 taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc ttaccgttga
    54721 cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat gaagccatca
    54781 cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct acccgttctc
    54841 cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca tagattttcc
    54901 cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg ggcaacacac
    54961 cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt aagatttttc
    55021 ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa ggtaataacc
    55081 cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa cacattgcat
    55141 caattactga cgaatttgac atcataatga acggttttta tttttcggca ataaaaacgt
    55201 aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag gagggaatag
    55261 aatggaagat tttttctttg caccatttga ttggatttat gggtggctta caggtaaata
    55321 gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca gtcttttttc
    55381 cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta ttttgcggga
    55441 tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac ttctggcaga
    55501 tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc catttttttc
    55561 gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc caaatgataa
    55621 cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc attttctttg
    55681 tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg gaccaatgtt
    55741 tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg atcaaattct
    55801 tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa atctacgaca
    55861 ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac agctgctaat
    55921 gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc ctcctctttt
    55981 gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc tgcttcttct
    56041 gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat tttttctgac
    56101 gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg cagatattca
    56161 cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc ttggtggcta
    56221 tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag cgaaggataa
    56281 tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg agtcaacata
    56341 gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt ccagtcagaa
    56401 cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg atgacgcgac
    56461 cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat tggcgatttg
    56521 tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca aatatacgga
    56581 aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg gtcatatctt
    56641 ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa tctggcataa
    56701 taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt ctttggtcac
    56761 cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca aataattttt
    56821 ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata tgttcttgat
    56881 ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc ccgtcccatt
    56941 cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat tttggcatca
    57001 cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt ttctcgattg
    57061 cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt ccttcaataa
    57121 agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct gatttacgtg
    57181 ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt aaaacagctc
    57241 catctatcac tggattgata aagacatttg agaaccagct tccagctaaa ggaccggttt
    57301 ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct aatacagctc
    57361 cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa cctggtgttt
    57421 ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat aatttttgca
    57481 ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca tctaaataag
    57541 cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac atgttcaaat
    57601 catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca ggaatcgttc
    57661 cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt ctcagtaagg
    57721 ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc caccaagccg
    57781 tcattttatc aaagtattct ttactagaat aatccataca taacactcct tttcattatt
    57841 actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct gttcatttgt
    57901 ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt ttcctttcgc
    57961 tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt tccgcagttc
    58021 atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa cacattgctg
    58081 gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat gtaaaagtct
    58141 ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt tctacaggga
    58201 ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata tcaataactt
    58261 caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt atggtgcagg
    58321 ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag attacaatct
    58381 agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat actactagaa
    58441 aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg acacgccgca
    58501 agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa aatcccgcaa
    58561 cccct
//
LOCUS       DACCQP010000010        58564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCQP010000010 DACCQP010000000
VERSION     DACCQP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.010"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            405..3239
                     /locus_tag="IVO87_000684"
     CDS             405..3239
                     /locus_tag="IVO87_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7448904.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3430..3921
                     /locus_tag="IVO87_000685"
     CDS             3430..3921
                     /locus_tag="IVO87_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7448905.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4024..5013
                     /gene="manX"
                     /locus_tag="IVO87_000686"
     CDS             4024..5013
                     /gene="manX"
                     /locus_tag="IVO87_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7448906.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5046..5849
                     /locus_tag="IVO87_000687"
     CDS             5046..5849
                     /locus_tag="IVO87_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7448907.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5870..6784
                     /locus_tag="IVO87_000688"
     CDS             5870..6784
                     /locus_tag="IVO87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7448908.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6867..7241
                     /locus_tag="IVO87_000689"
     CDS             6867..7241
                     /locus_tag="IVO87_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7448909.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7380..7625
                     /locus_tag="IVO87_000690"
     CDS             7380..7625
                     /locus_tag="IVO87_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7448910.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7797..8861
                     /locus_tag="IVO87_000691"
     CDS             7797..8861
                     /locus_tag="IVO87_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7448911.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9107..9532
                     /locus_tag="IVO87_000692"
     CDS             9107..9532
                     /locus_tag="IVO87_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7448912.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9525..9974
                     /gene="nusB"
                     /locus_tag="IVO87_000693"
     CDS             9525..9974
                     /gene="nusB"
                     /locus_tag="IVO87_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7448913.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10071..10925
                     /locus_tag="IVO87_000694"
     CDS             10071..10925
                     /locus_tag="IVO87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7448914.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10926..12263
                     /locus_tag="IVO87_000695"
     CDS             10926..12263
                     /locus_tag="IVO87_000695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7448915.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12268..12492
                     /locus_tag="IVO87_000696"
     CDS             12268..12492
                     /locus_tag="IVO87_000696"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7448916.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12494..13384
                     /locus_tag="IVO87_000697"
     CDS             12494..13384
                     /locus_tag="IVO87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7448917.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13393..14211
                     /locus_tag="IVO87_000698"
     CDS             13393..14211
                     /locus_tag="IVO87_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7448918.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14318..14767
                     /locus_tag="IVO87_000699"
     CDS             14318..14767
                     /locus_tag="IVO87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7448919.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14785..16467
                     /gene="recN"
                     /locus_tag="IVO87_000700"
     CDS             14785..16467
                     /gene="recN"
                     /locus_tag="IVO87_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7448920.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16522..16638)
                     /locus_tag="IVO87_000701"
     CDS             complement(16522..16638)
                     /locus_tag="IVO87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7448921.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16711..17667)
                     /locus_tag="IVO87_000702"
     CDS             complement(16711..17667)
                     /locus_tag="IVO87_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7448922.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17838..18206
                     /locus_tag="IVO87_000703"
     CDS             17838..18206
                     /locus_tag="IVO87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7448923.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18376..18807
                     /gene="mraZ"
                     /locus_tag="IVO87_000704"
     CDS             18376..18807
                     /gene="mraZ"
                     /locus_tag="IVO87_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7448924.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18828..19787
                     /gene="rsmH"
                     /locus_tag="IVO87_000705"
     CDS             18828..19787
                     /gene="rsmH"
                     /locus_tag="IVO87_000705"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7448925.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19792..20193
                     /gene="ftsL"
                     /locus_tag="IVO87_000706"
     CDS             19792..20193
                     /gene="ftsL"
                     /locus_tag="IVO87_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7448926.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20194..22386
                     /locus_tag="IVO87_000707"
     CDS             20194..22386
                     /locus_tag="IVO87_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7448927.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22412..23374
                     /locus_tag="IVO87_000708"
     CDS             22412..23374
                     /locus_tag="IVO87_000708"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7448928.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23388..24749
                     /locus_tag="IVO87_000709"
     CDS             23388..24749
                     /locus_tag="IVO87_000709"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7448929.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24775..25863
                     /gene="murG"
                     /locus_tag="IVO87_000710"
     CDS             24775..25863
                     /gene="murG"
                     /locus_tag="IVO87_000710"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7448930.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25878..27098
                     /locus_tag="IVO87_000711"
     CDS             25878..27098
                     /locus_tag="IVO87_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7448931.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27254..28579
                     /gene="ftsA"
                     /locus_tag="IVO87_000712"
     CDS             27254..28579
                     /gene="ftsA"
                     /locus_tag="IVO87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7448932.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28602..29843
                     /gene="ftsZ"
                     /locus_tag="IVO87_000713"
     CDS             28602..29843
                     /gene="ftsZ"
                     /locus_tag="IVO87_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7448933.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29860..30537
                     /locus_tag="IVO87_000714"
     CDS             29860..30537
                     /locus_tag="IVO87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7448934.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30551..31150
                     /locus_tag="IVO87_000715"
     CDS             30551..31150
                     /locus_tag="IVO87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7448935.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31165..31452
                     /locus_tag="IVO87_000716"
     CDS             31165..31452
                     /locus_tag="IVO87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7448936.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31501..32283
                     /locus_tag="IVO87_000717"
     CDS             31501..32283
                     /locus_tag="IVO87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7448937.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32304..33011
                     /locus_tag="IVO87_000718"
     CDS             32304..33011
                     /locus_tag="IVO87_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7448938.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33273..36071
                     /gene="ileS"
                     /locus_tag="IVO87_000719"
     CDS             33273..36071
                     /gene="ileS"
                     /locus_tag="IVO87_000719"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7448939.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36120..37646)
                     /locus_tag="IVO87_000720"
     CDS             complement(36120..37646)
                     /locus_tag="IVO87_000720"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7448940.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37661..38308)
                     /locus_tag="IVO87_000721"
     CDS             complement(37661..38308)
                     /locus_tag="IVO87_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7448941.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38492..38821)
                     /locus_tag="IVO87_000722"
     CDS             complement(38492..38821)
                     /locus_tag="IVO87_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7448942.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38998..39726
                     /gene="sfsA"
                     /locus_tag="IVO87_000723"
     CDS             38998..39726
                     /gene="sfsA"
                     /locus_tag="IVO87_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7448943.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39742..40755
                     /locus_tag="IVO87_000724"
     CDS             39742..40755
                     /locus_tag="IVO87_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7448944.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40755..42032
                     /locus_tag="IVO87_000725"
     CDS             40755..42032
                     /locus_tag="IVO87_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7448945.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42095..44797
                     /locus_tag="IVO87_000726"
     CDS             42095..44797
                     /locus_tag="IVO87_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7448946.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44949..45266
                     /locus_tag="IVO87_000727"
     CDS             44949..45266
                     /locus_tag="IVO87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7448947.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45296..45616
                     /locus_tag="IVO87_000728"
     CDS             45296..45616
                     /locus_tag="IVO87_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7448948.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45724..47184
                     /locus_tag="IVO87_000729"
     CDS             45724..47184
                     /locus_tag="IVO87_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7448949.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47252..47473
                     /locus_tag="IVO87_000730"
     CDS             47252..47473
                     /locus_tag="IVO87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448950.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47504..47686
                     /locus_tag="IVO87_000731"
     CDS             47504..47686
                     /locus_tag="IVO87_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7448951.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47686..48099
                     /locus_tag="IVO87_000732"
     CDS             47686..48099
                     /locus_tag="IVO87_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7448952.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48222..49403
                     /locus_tag="IVO87_000733"
     CDS             48222..49403
                     /locus_tag="IVO87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7448953.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49792..49865
                     /locus_tag="IVO87_000734"
     tRNA            49792..49865
                     /locus_tag="IVO87_000734"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49826..49828,aa:Arg,seq:cct)
     gene            complement(49934..51073)
                     /locus_tag="IVO87_000735"
     CDS             complement(49934..51073)
                     /locus_tag="IVO87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7448954.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51372..52007)
                     /locus_tag="IVO87_000736"
     CDS             complement(51372..52007)
                     /locus_tag="IVO87_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448955.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52120..52755)
                     /locus_tag="IVO87_000737"
     CDS             complement(52120..52755)
                     /locus_tag="IVO87_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7448956.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52789..53250)
                     /locus_tag="IVO87_000738"
     CDS             complement(52789..53250)
                     /locus_tag="IVO87_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7448957.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53380..53811)
                     /locus_tag="IVO87_000739"
     CDS             complement(53380..53811)
                     /locus_tag="IVO87_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7448958.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53829..54149)
                     /locus_tag="IVO87_000740"
     CDS             complement(53829..54149)
                     /locus_tag="IVO87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7448959.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54448..55224
                     /locus_tag="IVO87_000741"
     CDS             54448..55224
                     /locus_tag="IVO87_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7448960.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55239..55442
                     /locus_tag="IVO87_000742"
     CDS             55239..55442
                     /locus_tag="IVO87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448961.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55458..55796
                     /locus_tag="IVO87_000743"
     CDS             55458..55796
                     /locus_tag="IVO87_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7448962.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55783..55962
                     /locus_tag="IVO87_000744"
     CDS             55783..55962
                     /locus_tag="IVO87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448963.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(56005..56475)
                     /locus_tag="IVO87_000745"
     CDS             complement(56005..56475)
                     /locus_tag="IVO87_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448964.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56562..57260
                     /locus_tag="IVO87_000746"
     CDS             56562..57260
                     /locus_tag="IVO87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7448965.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57438..57779
                     /locus_tag="IVO87_000747"
     CDS             57438..57779
                     /locus_tag="IVO87_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448966.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57772..58443
                     /locus_tag="IVO87_000748"
     CDS             57772..58443
                     /locus_tag="IVO87_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7448967.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT        19513 a         9803 c        12257 g        16991 t
ORIGIN      
        1 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
       61 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
      121 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      181 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      241 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      301 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      361 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      421 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      481 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      541 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      601 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      661 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      721 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      781 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      841 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      901 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      961 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
     1021 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1081 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1141 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1201 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1261 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1321 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1381 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1441 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1501 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1561 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1621 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1681 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1741 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1801 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1861 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1921 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1981 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     2041 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2101 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2161 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2221 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2281 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2341 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2401 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2461 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2521 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2581 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2641 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2701 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2761 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2821 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2881 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2941 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     3001 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3061 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3121 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3181 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3241 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3301 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3361 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3421 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3481 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3541 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3601 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3661 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3721 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3781 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3841 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3901 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3961 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     4021 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4081 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4141 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4201 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4261 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4321 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4381 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4441 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4501 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4561 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4621 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4681 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4741 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4801 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4861 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4921 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4981 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     5041 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5101 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5161 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5221 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5281 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5341 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5401 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5461 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5521 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5581 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5641 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5701 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5761 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5821 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5881 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5941 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     6001 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6061 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6121 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6181 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6241 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6301 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6361 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6421 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6481 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6541 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6601 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6661 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6721 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6781 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6841 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6901 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
     6961 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     7021 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7081 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7141 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7201 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7261 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7321 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7381 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7441 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7501 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7561 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
     7621 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
     7681 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
     7741 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
     7801 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
     7861 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
     7921 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
     7981 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
     8041 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
     8101 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
     8161 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
     8221 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
     8281 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
     8341 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
     8401 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
     8461 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
     8521 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
     8581 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
     8641 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
     8701 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
     8761 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
     8821 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
     8881 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
     8941 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
     9001 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
     9061 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
     9121 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
     9181 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
     9241 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
     9301 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
     9361 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
     9421 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
     9481 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
     9541 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
     9601 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
     9661 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
     9721 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
     9781 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
     9841 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
     9901 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
     9961 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
    10021 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    10081 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    10141 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    10201 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    10261 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    10321 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    10381 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    10441 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    10501 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    10561 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    10621 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    10681 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    10741 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    10801 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    10861 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    10921 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    10981 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    11041 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    11101 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    11161 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    11221 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    11281 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    11341 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    11401 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    11461 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    11521 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    11581 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    11641 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    11701 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    11761 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    11821 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    11881 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    11941 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    12001 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    12061 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    12121 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    12181 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    12241 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    12301 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    12361 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    12421 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    12481 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    12541 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    12601 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    12661 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    12721 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    12781 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    12841 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    12901 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    12961 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    13021 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    13081 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    13141 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    13201 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    13261 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    13321 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    13381 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    13441 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    13501 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    13561 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    13621 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    13681 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    13741 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    13801 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    13861 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    13921 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    13981 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    14041 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    14101 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    14161 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    14221 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    14281 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    14341 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    14401 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    14461 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    14521 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    14581 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    14641 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    14701 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    14761 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    14821 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    14881 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    14941 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    15001 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    15061 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    15121 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    15181 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    15241 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    15301 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    15361 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    15421 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    15481 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    15541 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    15601 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    15661 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    15721 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    15781 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    15841 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    15901 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    15961 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    16021 gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    16081 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    16141 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    16201 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    16261 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    16321 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    16381 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    16441 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    16501 gctgatagct atattttatt tttacatgac actcattaca gcagtcaata caacagatcc
    16561 aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt
    16621 ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa
    16681 tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata
    16741 ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg catcatccct
    16801 ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt
    16861 taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat
    16921 tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat
    16981 tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg
    17041 gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct
    17101 gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga
    17161 tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt
    17221 gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc
    17281 actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat
    17341 tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa
    17401 ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt
    17461 caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt
    17521 gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg
    17581 acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc
    17641 attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat
    17701 aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata
    17761 aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa
    17821 aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc
    17881 cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt
    17941 ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt
    18001 ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg
    18061 gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt
    18121 tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca
    18181 ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa
    18241 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat
    18301 ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg
    18361 aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt
    18421 ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga
    18481 ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg
    18541 aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct
    18601 gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat
    18661 tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg
    18721 gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct
    18781 gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat
    18841 ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg
    18901 gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac
    18961 taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca
    19021 agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca
    19081 gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc
    19141 taggggtttc ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg
    19201 caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg
    19261 aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca
    19321 aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg
    19381 agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc
    19441 ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag
    19501 aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca
    19561 cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac
    19621 aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca
    19681 ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca
    19741 gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa
    19801 ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa
    19861 caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga
    19921 ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt
    19981 gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc
    20041 acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa
    20101 aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct
    20161 attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg
    20221 tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta
    20281 tttgctacga gtattggatt gttcttttta tttgccgtcc gtttctcgta tatcgttatt
    20341 ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt
    20401 gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa
    20461 gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa
    20521 aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt
    20581 tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat
    20641 caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa
    20701 gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg
    20761 tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag
    20821 aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa
    20881 gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca
    20941 gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa
    21001 agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca
    21061 gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc
    21121 aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta
    21181 caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa
    21241 gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct
    21301 acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca
    21361 tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac
    21421 tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac
    21481 ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta
    21541 ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg
    21601 attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact
    21661 caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat
    21721 atgagagatg ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct
    21781 ggatacaatg tatcggcaaa aaccggaaca gcccaaatcg cttctgatac agggggatat
    21841 caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca
    21901 gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct
    21961 aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga agaagattca
    22021 gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac
    22081 gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa
    22141 aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc
    22201 ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc
    22261 atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc
    22321 aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa
    22381 gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat
    22441 tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat
    22501 gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg
    22561 aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt
    22621 tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt
    22681 atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg
    22741 actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt
    22801 ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg
    22861 aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct
    22921 gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg
    22981 tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg
    23041 aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt
    23101 cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg
    23161 gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt
    23221 tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt
    23281 tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt
    23341 gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat
    23401 caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg
    23461 ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg
    23521 aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc
    23581 caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca
    23641 gtcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc
    23701 tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa
    23761 cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt
    23821 tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata
    23881 aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata
    23941 tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat
    24001 atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta
    24061 actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt
    24121 tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata
    24181 aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa
    24241 atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag
    24301 aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag
    24361 gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa
    24421 gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat
    24481 ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa
    24541 aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt
    24601 ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat
    24661 tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat
    24721 tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa
    24781 attttggtta caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat
    24841 tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta
    24901 gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt
    24961 aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt
    25021 caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat
    25081 gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa
    25141 caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct
    25201 ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat
    25261 cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg
    25321 gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat
    25381 caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct
    25441 tcaggagata gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt
    25501 agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg
    25561 gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg
    25621 gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac
    25681 gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca
    25741 gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa
    25801 caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa
    25861 taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt
    25921 ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag
    25981 agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt
    26041 ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa
    26101 tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt
    26161 tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt
    26221 tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata
    26281 gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct
    26341 ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt
    26401 atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag
    26461 ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa
    26521 acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc
    26581 ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa
    26641 caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca
    26701 cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta
    26761 ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata
    26821 tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca
    26881 ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg
    26941 atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt
    27001 ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata
    27061 atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag
    27121 aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt
    27181 aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga
    27241 gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc
    27301 tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa
    27361 cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc
    27421 gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag
    27481 tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa
    27541 cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt
    27601 tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga
    27661 tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg
    27721 tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc
    27781 cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc
    27841 tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac
    27901 ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt
    27961 tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa
    28021 aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga
    28081 tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc
    28141 agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt
    28201 agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga
    28261 cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct
    28321 gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga
    28381 agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt
    28441 agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta
    28501 cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt
    28561 tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata
    28621 ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg
    28681 ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag
    28741 acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata
    28801 ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg
    28861 aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg
    28921 gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg
    28981 cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt
    29041 ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa
    29101 ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag
    29161 cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt
    29221 acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga
    29281 tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct
    29341 cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag
    29401 gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag
    29461 caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa
    29521 tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc
    29581 gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc
    29641 aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg
    29701 aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat
    29761 ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac
    29821 cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag
    29881 aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt
    29941 acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct
    30001 ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta
    30061 tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa
    30121 ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa
    30181 atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag
    30241 gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc
    30301 tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag
    30361 caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt
    30421 tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg
    30481 attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg
    30541 aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga
    30601 tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac
    30661 taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga
    30721 aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa
    30781 gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc
    30841 ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc
    30901 aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt
    30961 agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg
    31021 aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac
    31081 accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt
    31141 tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa
    31201 cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca
    31261 ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt
    31321 ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc
    31381 ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat
    31441 ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt
    31501 ttggatgcaa atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta
    31561 ggcgattgga ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac
    31621 ccaagacaat cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg
    31681 ttttatggcg gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac
    31741 gaacctgtag aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt
    31801 gccagcctaa gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct
    31861 tactttggcg atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg
    31921 gatcattttg ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca
    31981 atcaaataca cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt
    32041 agttccatgc gactagacag tgtgatctct gagatttata atatttccag acagcggtcc
    32101 aagcagctga tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat
    32161 tttgtacttg aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa
    32221 gaacttgagg ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa
    32281 taaaataaaa aggtggttta ctcatggctt taactccatt agatatccaa aataagacat
    32341 tcccgacaaa aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg
    32401 ttcgtgacta tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt
    32461 ctgaagaaaa acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg
    32521 tagctcaaga tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg
    32581 ttacttctgc acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc
    32641 aattaacaac tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag
    32701 caagacagct tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac
    32761 gtatcagctt gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa
    32821 ttttacaacc attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag
    32881 caaaacagct tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag
    32941 cacctatgac ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg
    33001 aaaacgagta gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag
    33061 ttcggtaaga cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag
    33121 agtaagaagc agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca
    33181 actaaatttg ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg
    33241 tccggaaaac aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag
    33301 gaaaaacaaa attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag
    33361 aatgggaaga aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct
    33421 ttgttcttca tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga
    33481 ataaaatcag taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat
    33541 atgttccagg atgggataca catggattgc cgattgaaca agtattggct aataaaggcg
    33601 tgaaacgtaa agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt
    33661 cccaagtaga taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac
    33721 atccttatgt tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa
    33781 tggctgaaaa aggctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg
    33841 agtcttcttt agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg
    33901 tagctttcaa tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt
    33961 ggacgacaac accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta
    34021 cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa
    34081 cagtccaatc agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag
    34141 aaatggaata tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag
    34201 gcgatcatgt tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg
    34261 aagatgacta tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa
    34321 aaggattgtt tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc
    34381 ctatgatcac tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc
    34441 atagttatcc tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat
    34501 ggtttgcatc tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt
    34561 gggttatccc atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg
    34621 taatttcccg tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag
    34681 aagcaatcat cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat
    34741 caattatctg gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag
    34801 gttctcctaa tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg
    34861 gttcttctca tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt
    34921 atttagaagg atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg
    34981 ctatcaatgg tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg
    35041 aaggccgtaa gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac
    35101 aatttggtgc agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc
    35161 gtgtttctat ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga
    35221 tgcgtttctt attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca
    35281 atgatttacg ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa
    35341 tccgagaaga agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact
    35401 tcttgacggt tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg
    35461 aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag
    35521 ctttgacaaa attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc
    35581 tgaaagaaga agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa
    35641 accaagaaga attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa
    35701 aagcattgga agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa
    35761 ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac
    35821 aattattgat cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag
    35881 ataacgctat ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt
    35941 gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat
    36001 gcggtcgttg tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg
    36061 aagaaaagta aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat
    36121 caatcgttta attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat
    36181 ccgtcttttt caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg
    36241 ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat
    36301 tgagcgactt cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt
    36361 aatttttcgt aagcttctgg gctattttca accatttctg cactatttct aaattccaat
    36421 ttgattggtt ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat
    36481 ccttcagttg gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat
    36541 ggttcatcga cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc
    36601 cgagtccctt tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa
    36661 cggaagttat cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc
    36721 actttgtctt cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat
    36781 tgcccgcgaa cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat
    36841 gctttgatct tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca
    36901 agtaatgcta caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga
    36961 tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg
    37021 tcgatatagc gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag
    37081 atgttttgga tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg
    37141 aatacttctc tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt
    37201 ttttctatga tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga
    37261 gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt
    37321 aattgatagt gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa
    37381 tcatttacat catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct
    37441 gaaggattca atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag
    37501 ggacggcgag cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg
    37561 cggaacaaag acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta
    37621 aacaatacat ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa
    37681 tcgtgctggc cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg
    37741 gtccttcata agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag
    37801 tgactaagta atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac
    37861 cgacaggata ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag
    37921 gaatcgatcc tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg
    37981 caagatgttc cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc
    38041 tatagtctaa atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc
    38101 tagctttttg cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa
    38161 gctcttcttt cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac
    38221 tagatacgat ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt
    38281 tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc
    38341 attagtaagt gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga
    38401 tttttagacg tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt
    38461 ataaaggata tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag
    38521 tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat
    38581 actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact
    38641 gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat
    38701 cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt
    38761 tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca
    38821 ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct
    38881 tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt
    38941 ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg
    39001 aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat
    39061 tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa
    39121 gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa
    39181 accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa
    39241 ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta
    39301 actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt
    39361 tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa
    39421 aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga
    39481 gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc
    39541 gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt
    39601 cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat
    39661 gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata
    39721 caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact
    39781 catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct
    39841 agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca
    39901 acagatttga agactttttt caatattact catatggaca ccatttatat tgcttctccg
    39961 aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt
    40021 gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa
    40081 cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt
    40141 gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat
    40201 tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc
    40261 tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt
    40321 ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt
    40381 ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt
    40441 gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc
    40501 aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg
    40561 gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct
    40621 catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat
    40681 gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat
    40741 gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat
    40801 atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa
    40861 gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc
    40921 tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt
    40981 cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag
    41041 agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga
    41101 aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa
    41161 aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg
    41221 acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc
    41281 tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga
    41341 aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt
    41401 actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag
    41461 aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa
    41521 aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc
    41581 gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg
    41641 tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt
    41701 taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa
    41761 tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat
    41821 caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa
    41881 ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg
    41941 agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa
    42001 aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa
    42061 cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt
    42121 gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac
    42181 tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt
    42241 aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga
    42301 agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc
    42361 tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca
    42421 tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca
    42481 ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc
    42541 ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc
    42601 ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt
    42661 gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat
    42721 tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca
    42781 gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat
    42841 tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat
    42901 catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa
    42961 agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg
    43021 agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa
    43081 gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa
    43141 ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga
    43201 accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa
    43261 cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga
    43321 gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa
    43381 aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta
    43441 tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc
    43501 tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga
    43561 tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac
    43621 gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat
    43681 gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct
    43741 catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt
    43801 aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta
    43861 tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt
    43921 gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat
    43981 tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc
    44041 aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat
    44101 cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat
    44161 cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt
    44221 gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa
    44281 aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga
    44341 tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact
    44401 aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact
    44461 tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac
    44521 agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat
    44581 cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt
    44641 tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa
    44701 cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct
    44761 tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca
    44821 cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca
    44881 gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag
    44941 tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt
    45001 tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg
    45061 cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat
    45121 taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta
    45181 ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg
    45241 cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga
    45301 agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag
    45361 cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa
    45421 aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac
    45481 acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca
    45541 cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg
    45601 cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg
    45661 attttttatt ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa
    45721 aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc
    45781 ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca
    45841 atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt
    45901 aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc
    45961 atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt
    46021 gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg
    46081 atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca
    46141 ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca
    46201 acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg
    46261 aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt
    46321 aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct
    46381 aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat
    46441 tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt
    46501 gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt
    46561 gttcaagtat tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag
    46621 ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga
    46681 aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc
    46741 cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg
    46801 atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt
    46861 gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat
    46921 gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca
    46981 gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg
    47041 ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta
    47101 tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac
    47161 cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt
    47221 cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca
    47281 agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa
    47341 agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc
    47401 taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc
    47461 aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt
    47521 atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag
    47581 gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt
    47641 catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa
    47701 aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat
    47761 gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc
    47821 aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag
    47881 tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa
    47941 ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg
    48001 cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt
    48061 ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat
    48121 ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc
    48181 tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag
    48241 agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg
    48301 cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg
    48361 gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga
    48421 atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag
    48481 caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc
    48541 gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct
    48601 atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca
    48661 aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa
    48721 gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt
    48781 atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg
    48841 aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt
    48901 ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg
    48961 gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat
    49021 tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata
    49081 tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa
    49141 tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg
    49201 gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc
    49261 aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa
    49321 gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc
    49381 caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc
    49441 ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa
    49501 tttgaatttt ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc
    49561 tttcgtaatc ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc
    49621 ttttggctta tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt
    49681 atcatctgtt aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca
    49741 gataagcttg caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta
    49801 gctcagtagg atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag
    49861 gaacgtataa ataacccctg tatcctttgc ggtacagggg tttgttttgt atttgtgcca
    49921 aaaatgtgcc aaactagtta tcatttttat ataaactatc gaacacagta ttgatttttt
    49981 ctttatcctg atcttcaagc tctcgaacaa tatgaagata agtagacatt gtagtttcta
    50041 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
    50101 tagatgcatg tgtatgcctt agtccatgca aagtaaattg cttttcaatc cctaattctt
    50161 ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga aactaatcca ttttttaagt
    50221 tgaaaaaaac aaaattatga gggtttttga tttcaaaatt ctcgaataat tctttttgat
    50281 tgattttaaa cttttttaag agatcaatcg tatgtttatc aatggaaatt ttcctatttg
    50341 aagtttcgtt ttttgttttt ccccattcat ttaatttata attccatgtt ttattaatag
    50401 tgatgatttg atcttcgaaa tcgatatctt cccatgttaa tcctagaagt tcagcaaatc
    50461 gcattccagt ggcaccagca actaacacga gcataggaga agagtactgc gcagaaaggt
    50521 tttcttctac gagtttcatt aaagatttaa attgatcata atctaaatat ttatcttctt
    50581 ttggcttcaa tgaatctttt cctttgatta ctgcttttct agttggatca aacggaatta
    50641 atccttcttc tacggcatct ttcaacgacg ctctaatatg gttattaaac ttaataacgg
    50701 tagattttac atggtcttta gcatacttat ttaaaaagcg ttgatatccc actctatcta
    50761 aatcagaaat caataccgct ggcatatatt tctttatgtt cattaacgta tcttcgtatt
    50821 ttctataagt gataggagat actgttcctt ctttataaag ttgcatccaa tcctcaaagt
    50881 aatctgataa taatagattt ttgcgatccc ctttaaggcc tttgttcaag ttatattcta
    50941 attcgttggc ggcatctttt gcttctcctt ttgtcttaaa tcctgatttt cttatcttcg
    51001 tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg ccaactattt ccacgtttga
    51061 ctaatcttgc cataattgaa tcagctctct ttctctgata caataggaac tataaagaag
    51121 cccattgttt gggttttatt attttttgca catccttttt gcttgccggc gcaaggatgt
    51181 gctatttgtc gttttaatgg tataattgga tgtgtattag atcatccaat cagaggtgtt
    51241 tattatgatt aaagtgattt ttaaaagaat aactgaactt ccagagttta gattatttgt
    51301 tgattattgg tatgtctggt tttttattgt tgcattgctg ataggtctag atgtcatatt
    51361 agaaagaaaa cttaagtaag agttgagtta ataaattgaa ttcctaaatg agatattttg
    51421 tagcccatat tttgtttgcc gacatcacct aatactatgg agctaatcgt tttttctaac
    51481 agaccaaaat cagataatct atttaagcta ccatctatta gctcttgtga atagccagac
    51541 atgctgatat ttccagtgaa attttcgttt ccacgactca ttaagcatgc taacaattga
    51601 atctcaatcg atgttaattg attcagtaca agaagataat actcctcgtt atcccatgaa
    51661 gcgttgttaa ttcgaagtaa acaattctta taaagatttt tatacatatc tattttattt
    51721 tgtgcctttg atttttctac ctcgtcattt atcccttcga atattccaat gagttggtcc
    51781 ttgttctcta agttgaaaac atcttctgga atttgatcgc gaatgttttc taatgattcg
    51841 tttaaatttt tgtagaattt ctcgatgcgt ttgaaacgct tctcgttttt agacccaaaa
    51901 taaagtgact gcacaacgcc accaatataa ggaattgctc caataccgcc ttcaactatt
    51961 aattcaagat attctttttt ggttagtttt tcattgctat tatccatagt tacctcctat
    52021 tttgtcattt cctttacaag cattgtgact cctgatggca ctcttttgta taggttttat
    52081 tattgtgcac aatctaactc ttagtggggc gtggttcatt tattttttac ttactttact
    52141 attaatcata tcccattgag cataggattt ttctatgttc gatcctcttg aactaatttc
    52201 tcctgtaaaa gttactttat cgccctttgt aaaggtatca gcattgttta agtccttcgc
    52261 aaaaattaca tatgaaactt taggtgttgc agaaatatca ttccaacttt tatcatcgta
    52321 ctctttgtct gctataatgg ctattctagt agtcattgat tcgataacag ttccttcaaa
    52381 agtatatgtt ttaccactaa tatatttagt aaaagctgta ttttgctcag tagctggagt
    52441 aatagaataa taactatcta agaaagcacc ccagtcatga ttatttgtct ctaaaaattc
    52501 ttcaaattga gttaggcttt tattgctgct gttagatgta gtagtttgag tatcagatga
    52561 tgagctttcc tgtttgttat tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac
    52621 ggtagtaaaa ataatcccga taataaggag aatcataggt gttttcttat ttgttttttt
    52681 gataaatctt acaattaaca tgattaaacc tataataaat aaaacaatac cagtaaaacc
    52741 aaatataccg tccattaata gatcctcttt ctcttttgat attgaatatt ataaaccaag
    52801 caattctttt ttcttaaaat cgaattcttc ttgattaata atcccttcat ctaacaactc
    52861 tttatacttt ttaatttcat ctgcacttga aatttgtgtt tctttggaat tattatctaa
    52921 tatagattgt tgcttctcta tatatttttt taaattttcc atttcattcc aatatttttt
    52981 tgtaaacata acggtgtttt catctttcgt ggccgctaat atacctccgc gactttcgtt
    53041 tccgcctagc aaggtaaatt gtatataacc attactcata cctggttttt ttaattgtac
    53101 ggctgatata tttttaaagg ggatagtttt ttctccttta ataccttgat taactaaatt
    53161 tataaatcct tttctaataa ttgaaataga atcatcagtt actttaatca aggtttttcc
    53221 tggagatttt attaaaatct ctttttccat tttttcttcc tcatttctgt gatatgattt
    53281 tttttgtaga agctcatata tgaggaaaga gcccgtgttg cagcacgggc ttttttaatg
    53341 ttttgctgaa atcggttttt ttaataattc ttgatgtatt catacatatt gccttgcgta
    53401 agaatatttt ttcttaaaat agcattggca gacagcataa caagtgtatc tgagcttatt
    53461 ctgaatagaa taatactcca taaatttttc gagattaaac tgagattcat cagtcagttc
    53521 gttctcaata tagatattta ataaaattag aatagctatt ttatctgctt ctgtttcaaa
    53581 ttttgagtga aaagttgtag aagtatcgta caaaactgaa aattcaaaat gagaggcgct
    53641 gaaatgtgca agttcatgag ataaatgaaa ggcttctgca gtttcaccgt atagattttc
    53701 attcaaaaaa atgattctgg gttttggata gtagaaacca ggctctttca tctccatata
    53761 aactactttc aaattgtatt cgctcaacat ttctttcaat ttcaaataca tacaaaccat
    53821 cactccaact attcattttc ctctaaagct ttagcaattg caatcgcttt acgcattgtc
    53881 tccttagata tttcttttcc atcaaaagaa aaaacagtat cgtcttctga taaatccaca
    53941 tgtttagggg tttctctttc ttctctacct agaaggtagt ctacagagac atcgaaatag
    54001 tcagcaatcc gacttaattc atctgaatta ggagtattat ttttccactt tgctagataa
    54061 ccgttagaat atccaaaatt aatttctaac tgccgtatag atatcttttt cctcttcgcc
    54121 aagtctttta ttatttcata ggtattcatc gataaaacaa cctttctaaa tgtttacaaa
    54181 aaaagtttag aaaaatacgc agaaataatt tgactaattc tgagtaatga gctatactat
    54241 atcttgtaaa cagatttaac aactaaaaag acaacaaaaa acactattga tatgtaaatg
    54301 cagaccgcca agaaagcttt aaaatcaatg tttttatgtc ttatttaatt atgctcttag
    54361 tatagagtat tactcagata ctgtcaattg aatttagaaa aaagttgtta aatttgttta
    54421 cgaatataaa agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa
    54481 aacgaagttc ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg
    54541 agtgttttgg gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat
    54601 aagatggggt cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa
    54661 tctggtttgt acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt
    54721 tgggtaacaa gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa
    54781 aaagtagaag aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa
    54841 gaagaaagaa ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc
    54901 tcctatttag atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca
    54961 gattatggga tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa
    55021 aaagtcggta accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct
    55081 catacaactg agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg
    55141 acacagaaag ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa
    55201 atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca
    55261 ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca
    55321 ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta
    55381 aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg tacgaattgt
    55441 aaagggagga acagctaatg caatatctag aagcaaaaat cccaattcca gaaggctatg
    55501 taattatctc ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga
    55561 tgacgttgcc agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag
    55621 ttttaaagaa cccaagatat agaaatatta tcgatatcga gaaaaacaaa aatggatttg
    55681 tctattatcc agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat
    55741 ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga
    55801 gcgctagtgt tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcaggcaa
    55861 gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa
    55921 gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga
    55981 aactagaatg tatattacta ttactcagtt taattctttc actaatttct ctattgggaa
    56041 gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag taaatatcat
    56101 ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa atgcaaggta
    56161 acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt atattctatt
    56221 atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg accatctgcg
    56281 gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg ttgaatagtt
    56341 agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat taagttcaac
    56401 ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac tcaatataaa
    56461 acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac
    56521 cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt
    56581 tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc gagaaaaaac
    56641 atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat tttggacaga
    56701 tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt ttcttcatta
    56761 gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct atctacttca
    56821 aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag aatactgttc
    56881 ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat gttaaaaaag
    56941 atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc
    57001 aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga gcttatgaag
    57061 aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc
    57121 caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt
    57181 ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa cagagacttt
    57241 ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat tgctaattgc
    57301 tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca
    57361 tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca
    57421 taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat ttgattgatc
    57481 ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt
    57541 tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta actatttggg
    57601 atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat atttacgtca
    57661 tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg attgatatct
    57721 cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa
    57781 atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag
    57841 aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat
    57901 gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt ttctggaact
    57961 catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac tgggacttat
    58021 gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc tgttttagat
    58081 tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc attgaagcga
    58141 tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca aggggaagat
    58201 attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac gattctagaa
    58261 gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata tgttaatgag
    58321 cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca gttaagctat
    58381 gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt agataagaag
    58441 tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa aagttcaaag
    58501 gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta aagacgatgt
    58561 atga
//
LOCUS       DACCQP010000011        56188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCQP010000011 DACCQP010000000
VERSION     DACCQP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.011"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            36..995
                     /locus_tag="IVO87_000749"
     CDS             36..995
                     /locus_tag="IVO87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7448968.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1165..1845
                     /locus_tag="IVO87_000750"
     CDS             1165..1845
                     /locus_tag="IVO87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7448969.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1935..2969
                     /locus_tag="IVO87_000751"
     CDS             1935..2969
                     /locus_tag="IVO87_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7448970.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2983..3318
                     /locus_tag="IVO87_000752"
     CDS             2983..3318
                     /locus_tag="IVO87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448971.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3302..5782
                     /locus_tag="IVO87_000753"
     CDS             3302..5782
                     /locus_tag="IVO87_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448972.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5838..7397
                     /locus_tag="IVO87_000754"
     CDS             5838..7397
                     /locus_tag="IVO87_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7448973.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7394..8758
                     /locus_tag="IVO87_000755"
     CDS             7394..8758
                     /locus_tag="IVO87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7448974.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8929..9999
                     /locus_tag="IVO87_000756"
     CDS             8929..9999
                     /locus_tag="IVO87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448975.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10014..10682
                     /locus_tag="IVO87_000757"
     CDS             10014..10682
                     /locus_tag="IVO87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448976.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10702..11280
                     /locus_tag="IVO87_000758"
     CDS             10702..11280
                     /locus_tag="IVO87_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7448977.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11681..12592
                     /locus_tag="IVO87_000759"
     CDS             11681..12592
                     /locus_tag="IVO87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7448978.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12970..13887)
                     /locus_tag="IVO87_000760"
     CDS             complement(12970..13887)
                     /locus_tag="IVO87_000760"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7448979.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14089..14526
                     /gene="zapA"
                     /locus_tag="IVO87_000761"
     CDS             14089..14526
                     /gene="zapA"
                     /locus_tag="IVO87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7448980.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14584..15132
                     /locus_tag="IVO87_000762"
     CDS             14584..15132
                     /locus_tag="IVO87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7448981.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15247..17607
                     /locus_tag="IVO87_000763"
     CDS             15247..17607
                     /locus_tag="IVO87_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7448982.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17694..18008
                     /gene="trxA"
                     /locus_tag="IVO87_000764"
     CDS             17694..18008
                     /gene="trxA"
                     /locus_tag="IVO87_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7448983.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18190..19968
                     /gene="uvrC"
                     /locus_tag="IVO87_000765"
     CDS             18190..19968
                     /gene="uvrC"
                     /locus_tag="IVO87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7448984.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20113..21012
                     /locus_tag="IVO87_000766"
     CDS             20113..21012
                     /locus_tag="IVO87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7448985.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21005..22267
                     /locus_tag="IVO87_000767"
     CDS             21005..22267
                     /locus_tag="IVO87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7448986.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22428..24326
                     /locus_tag="IVO87_000768"
     CDS             22428..24326
                     /locus_tag="IVO87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7448987.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24656..25375)
                     /locus_tag="IVO87_000769"
     CDS             complement(24656..25375)
                     /locus_tag="IVO87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7448988.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            25712..26044
                     /locus_tag="IVO87_000770"
     CDS             25712..26044
                     /locus_tag="IVO87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7448989.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26031..28034
                     /locus_tag="IVO87_000771"
     CDS             26031..28034
                     /locus_tag="IVO87_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7448990.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28053..28523
                     /locus_tag="IVO87_000772"
     CDS             28053..28523
                     /locus_tag="IVO87_000772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7448991.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28544..29128
                     /locus_tag="IVO87_000773"
     CDS             28544..29128
                     /locus_tag="IVO87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7448992.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29143..30129
                     /locus_tag="IVO87_000774"
     CDS             29143..30129
                     /locus_tag="IVO87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7448993.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30119..30430
                     /locus_tag="IVO87_000775"
     CDS             30119..30430
                     /locus_tag="IVO87_000775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7448994.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30452..32233
                     /locus_tag="IVO87_000776"
     CDS             30452..32233
                     /locus_tag="IVO87_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7448995.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32226..33602
                     /locus_tag="IVO87_000777"
     CDS             32226..33602
                     /locus_tag="IVO87_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7448996.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33609..34244
                     /locus_tag="IVO87_000778"
     CDS             33609..34244
                     /locus_tag="IVO87_000778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7448997.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34274..35629
                     /locus_tag="IVO87_000779"
     CDS             34274..35629
                     /locus_tag="IVO87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7448998.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35821..37215)
                     /locus_tag="IVO87_000780"
     CDS             complement(35821..37215)
                     /locus_tag="IVO87_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7448999.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37307..38278
                     /locus_tag="IVO87_000781"
     CDS             37307..38278
                     /locus_tag="IVO87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7449000.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38275..39057
                     /locus_tag="IVO87_000782"
     CDS             38275..39057
                     /locus_tag="IVO87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7449001.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39226..39597)
                     /locus_tag="IVO87_000783"
     CDS             complement(39226..39597)
                     /locus_tag="IVO87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7449002.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39731..40174
                     /locus_tag="IVO87_000784"
     CDS             39731..40174
                     /locus_tag="IVO87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7449003.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40217..41026)
                     /gene="yidA"
                     /locus_tag="IVO87_000785"
     CDS             complement(40217..41026)
                     /gene="yidA"
                     /locus_tag="IVO87_000785"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7449004.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41045..42412)
                     /locus_tag="IVO87_000786"
     CDS             complement(41045..42412)
                     /locus_tag="IVO87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7449005.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42396..43226)
                     /locus_tag="IVO87_000787"
     CDS             complement(42396..43226)
                     /locus_tag="IVO87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7449006.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43389..43826
                     /locus_tag="IVO87_000788"
     CDS             43389..43826
                     /locus_tag="IVO87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7449007.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43902..44522
                     /locus_tag="IVO87_000789"
     CDS             43902..44522
                     /locus_tag="IVO87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7449008.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44711..45217
                     /locus_tag="IVO87_000790"
     CDS             44711..45217
                     /locus_tag="IVO87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7449009.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45234..46133
                     /locus_tag="IVO87_000791"
     CDS             45234..46133
                     /locus_tag="IVO87_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7449010.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46220..47104
                     /locus_tag="IVO87_000792"
     CDS             46220..47104
                     /locus_tag="IVO87_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7449011.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47296..47367)
                     /locus_tag="IVO87_000793"
     tRNA            complement(47296..47367)
                     /locus_tag="IVO87_000793"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47332..47334),aa:Arg,seq:ccg)
     gene            47427..48761
                     /gene="rpoN"
                     /locus_tag="IVO87_000794"
     CDS             47427..48761
                     /gene="rpoN"
                     /locus_tag="IVO87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7449012.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            48964..50001
                     /locus_tag="IVO87_000795"
     CDS             48964..50001
                     /locus_tag="IVO87_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7449013.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50042..51043
                     /gene="gap"
                     /locus_tag="IVO87_000796"
     CDS             50042..51043
                     /gene="gap"
                     /locus_tag="IVO87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7449014.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51153..52343
                     /locus_tag="IVO87_000797"
     CDS             51153..52343
                     /locus_tag="IVO87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7449015.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52447..53202
                     /locus_tag="IVO87_000798"
     CDS             52447..53202
                     /locus_tag="IVO87_000798"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7449016.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53318..54616
                     /gene="eno"
                     /locus_tag="IVO87_000799"
     CDS             53318..54616
                     /gene="eno"
                     /locus_tag="IVO87_000799"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7449017.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(54812..56107)
                     /locus_tag="IVO87_000800"
     CDS             complement(54812..56107)
                     /locus_tag="IVO87_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7449018.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT        18594 a         9811 c        11823 g        15960 t
ORIGIN      
        1 agttccgacg aaagaattat aaggagtgag tacaaatgac ctataaccat cttacaccaa
       61 ctgaactcgt aatgatagaa gcttatttca atcaatcaca acctgtttca aaagtagcca
      121 atctgttaca acgttctcga caaactattt ataaggtcta tcgttttctt aaatctggtg
      181 gaagcgcaat gagctactat aaacaatata agaaaaacaa acgtaactgc ggaagacatc
      241 cgattgtttt acctgatgag caacaggaat acattcaaaa aaaggttgtt caagggtggg
      301 ctcctgatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta cgcacactgt
      361 atcgaatgtt taaagataaa acctttgaca ctcacgattt accaatgaag ggaaaaagaa
      421 aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt tcgccggtca atccgagaca
      481 gaaagacaga ctatacccag tttgatcatg aatttggtca cctggaaggt gacactatcg
      541 tgggtggaaa acataaaagc gcggtcatca cattagttga acggctatct aaagtcatca
      601 ttaccttgaa acccgaaggc agacaggcta aagacattga aaataggctg aatcaatggt
      661 ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt tgattgtgga aaagaattct
      721 caaattggaa aaagattagt aataccaacg atatttcaat ttattttgcg gatccaggaa
      781 caccttctca acgaggattg aatgaacatt caaatggctt attacgtaaa gatggtttgt
      841 ataaagctat ggattttaat ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac
      901 gaaatcatat tcctagaaaa tcattacact atcggacacc aatggaagta tttttgagtt
      961 acgtcacaaa agatgatttg tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa
     1021 gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt
     1081 tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc
     1141 actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca
     1201 acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa
     1261 ggagatatta cctttactga accaacaaat accgtggagc ctcttaaccc tactgatcct
     1321 tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac aggtccttta
     1381 acactggatg tagttcccga attgccattt ggtacacacg agattgaaag cggaacaaaa
     1441 acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga
     1501 ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt
     1561 gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa
     1621 agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa
     1681 gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt
     1741 tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc
     1801 acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt
     1861 caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa
     1921 ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa
     1981 gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa
     2041 tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag
     2101 tggcaccgga acaaaatcag acactagaag taattatcag caattcttca gatgaagaac
     2161 gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata
     2221 gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca
     2281 ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactgtacct atagaagtga
     2341 aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag
     2401 aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata
     2461 atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat
     2521 caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc
     2581 agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt
     2641 atattgaaaa tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc
     2701 ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat
     2761 caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac
     2821 aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg
     2881 caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca
     2941 agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta
     3001 ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat
     3061 accaaaagtt caggtacgat tacattttat gatctaagtc caattccgca tccaagtggt
     3121 tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa
     3181 catggcgtat tcccggagac gaatgaacaa aagatccgtt ttctttattt gatcattggt
     3241 atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa
     3301 catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt
     3361 tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat
     3421 ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt
     3481 caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac
     3541 ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga
     3601 aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc
     3661 atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat
     3721 tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggaag
     3781 tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac
     3841 tctattgaca gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc
     3901 gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga
     3961 tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc
     4021 cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc
     4081 gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc
     4141 aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcacc
     4201 aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa
     4261 taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg
     4321 acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa
     4381 aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca
     4441 gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc
     4501 tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc
     4561 cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc
     4621 agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa
     4681 attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt
     4741 aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg
     4801 cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga
     4861 acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc
     4921 agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct
     4981 atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat
     5041 aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt
     5101 aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc
     5161 agatctattg agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac
     5221 tgtagatact tctttacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa
     5281 tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac
     5341 aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg
     5401 taacgatctc cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg atttttcttt
     5461 tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag gtacattgac
     5521 ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca
     5581 ttctggatca aagcaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg
     5641 aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt
     5701 aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact
     5761 gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca
     5821 gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag
     5881 gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt
     5941 tccaactttt attagaggaa aaaggagagg tttccgttaa taacgtactt acccgcttat
     6001 cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata
     6061 tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata
     6121 tagcagcagt cgatgtttat tatcattgtt acttgcgcta ttctacaaaa tatcagatac
     6181 ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata
     6241 tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc
     6301 atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaattcgt tatttttatt
     6361 tccaattatt ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac agtgatttat
     6421 caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttgctatt acagaagaag
     6481 gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa
     6541 aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat
     6601 tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa
     6661 atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat acacataact
     6721 cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga
     6781 atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga
     6841 tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag
     6901 gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata
     6961 aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta
     7021 ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttac
     7081 ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa
     7141 ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat
     7201 atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga
     7261 aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt
     7321 atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa
     7381 ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgaaggca tcacagctgc
     7441 cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa
     7501 agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag
     7561 tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac
     7621 cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga
     7681 aggaaaaaac gagaccataa cctatgataa attaatcgta gcgacaggtt cctctcctgt
     7741 caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc
     7801 taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta
     7861 cattggattg gaattagcag atgctttgag aaataaagga tacattattc atctagttga
     7921 ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa
     7981 tcagctgcaa actaagcaaa taaattttta tccaaatgaa ttcttgatcg attggaaaaa
     8041 atcagaagag aaagtagtat ccgtacaact tctgtctcaa acaataaagg cagatatggt
     8101 cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta
     8161 tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc
     8221 gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt
     8281 agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac
     8341 cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac
     8401 tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg
     8461 agaatttgat ctatttccac aatacgacga agaaaacaaa acagtaaatg caaagctgat
     8521 ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag aattcttatt
     8581 atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc
     8641 agttgaggat tttggatttc tgtctgaata cacctctcgt tttcactacc tcaatgagtt
     8701 agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga
     8761 aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa
     8821 attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca
     8881 agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca
     8941 ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg
     9001 atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga
     9061 acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat
     9121 ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg
     9181 tatcttgccc aaacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc
     9241 aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga
     9301 aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt
     9361 ctagccatag gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc
     9421 tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac
     9481 caagaaatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt
     9541 gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc
     9601 atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt
     9661 ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag
     9721 attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct
     9781 gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca
     9841 gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt
     9901 ggcgtcatta gcttgttgat gttagtagta gctgtgttag gcgcactatt ttctgttcgg
     9961 acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag aacatgaaag
    10021 caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat
    10081 tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg
    10141 gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct
    10201 ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagcgaacgc gttaaactac
    10261 gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa
    10321 aaaagcagtt attattagta gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg
    10381 aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg
    10441 gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc
    10501 tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct
    10561 tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc
    10621 tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat
    10681 aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt
    10741 ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagaa
    10801 atcgcacaaa aagttgggat cacccagccc aatctctatc attatttcgg tgataaagaa
    10861 aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa
    10921 atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg
    10981 attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca
    11041 aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa
    11101 gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca
    11161 aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa
    11221 ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa
    11281 agctgttata tagggagaaa atagttaggt gaagaagcaa gatatgacga agatcgttat
    11341 atcttgcttc tttatatagg tagtttttca taaatacaaa tttgttattc tctcatgatt
    11401 atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa tttaataacg
    11461 aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga
    11521 atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg
    11581 ttattttcta cggttatatc ttttacagcc ccttttttgc ttacttaagc tgattttaag
    11641 ttttggccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc
    11701 ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa
    11761 aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc
    11821 atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata
    11881 tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt
    11941 tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt
    12001 agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc
    12061 tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa
    12121 tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc
    12181 gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct
    12241 tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc
    12301 agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca
    12361 agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag
    12421 aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat
    12481 cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca
    12541 gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag
    12601 cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat
    12661 gcaagctttt aaacaccgtt taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca
    12721 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac ttgtgaccga
    12781 ggagttggca tgagaacacc gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag
    12841 tattagagaa acaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
    12901 gaggatcaga tgattgaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc
    12961 acagttccat tattttttta gtagcttcaa agctttttct gtattggcaa aatgaagttt
    13021 ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc
    13081 agatttcgtt ccagctcctg acggaagggt gattccagct tcagctgatg ctttgtctaa
    13141 ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg
    13201 ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata
    13261 ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg
    13321 ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac
    13381 ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata
    13441 ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt
    13501 tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc
    13561 acatactgtt agcggaccga aatagctgcc atttccgacc tcatcactac ctaaaacgga
    13621 taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc
    13681 ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt
    13741 tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg
    13801 aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt
    13861 tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata
    13921 ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt
    13981 tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt
    14041 catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa
    14101 aaaacacgct acaaagcggt gattgccaac caaacatata ctattatcgg acgggaaacc
    14161 aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa
    14221 ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg
    14281 gatcaattga agaaacaaga aagaatattg gagctggaag aagaaacagc agaattaaag
    14341 aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag
    14401 aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat
    14461 gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct
    14521 aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt
    14581 agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt
    14641 cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc
    14701 cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc
    14761 accccaacat caaagttagc attttttgat caggtatttg cctttcacct agatgaagct
    14821 ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt
    14881 gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt
    14941 gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta
    15001 cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt
    15061 tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat
    15121 attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat
    15181 ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt
    15241 gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc
    15301 agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt
    15361 gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt
    15421 ctgcgaggcg ggatccctat acctaaaatt gaaaatatcc gtccccatat gaaacggatt
    15481 gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg
    15541 acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt
    15601 ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa
    15661 gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga
    15721 cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg
    15781 aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc
    15841 ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca
    15901 ggtcagactt tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa
    15961 taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt
    16021 gttccccatc gtcaagaaat catccacaat acttatgtga ttggtaaaat ggatctgatg
    16081 aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac
    16141 aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct
    16201 aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt
    16261 gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta
    16321 ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata
    16381 ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact
    16441 gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca
    16501 ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa
    16561 aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatataat
    16621 cgggcaaata cgattaatgc cagcatggag tttgatgtgg atacattaag tccaacgtat
    16681 cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt
    16741 cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta
    16801 aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa
    16861 atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc
    16921 tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc
    16981 gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag
    17041 cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca
    17101 aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa
    17161 aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt
    17221 ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc
    17281 gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct
    17341 gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac
    17401 gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct
    17461 caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat
    17521 cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat
    17581 ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc
    17641 caagtataga aatgctatac tagtcataga ttttaaaaat ggaggtcttt gatatgtcac
    17701 aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt
    17761 tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac
    17821 aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa
    17881 ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag
    17941 tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat
    18001 atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc
    18061 ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt
    18121 gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg
    18181 gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc
    18241 tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta
    18301 aaaaatcgtg tccgctcata ttttactggt agccataata cgaaaacgga acggcttgtt
    18361 agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta
    18421 gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa
    18481 acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaag
    18541 gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag
    18601 acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag
    18661 gagccttgtc tttactatca tttgggacaa tgcctgtgtc cttattattt cgacgtagat
    18721 ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa
    18781 aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa
    18841 aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa
    18901 atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga taaaggatgg
    18961 atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg
    19021 tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag
    19081 gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt
    19141 gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta
    19201 gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc
    19261 agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg
    19321 accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct
    19381 gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata
    19441 aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat
    19501 tcgcgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa
    19561 ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt
    19621 gctggcatgg taaaaaacga taaacataaa acaaatgagt tgctattcgg tacagatttg
    19681 gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat
    19741 gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct
    19801 tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag
    19861 ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg
    19921 ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact
    19981 ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt
    20041 gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga
    20101 aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct
    20161 gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac
    20221 ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga
    20281 gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt
    20341 ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca
    20401 gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa
    20461 tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa
    20521 aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca
    20581 ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa
    20641 aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc
    20701 gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt
    20761 cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta
    20821 gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc
    20881 ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt
    20941 cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa
    21001 atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat
    21061 ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg
    21121 aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca
    21181 aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc
    21241 tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc
    21301 aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac
    21361 acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag cgagtcaatt
    21421 aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa
    21481 agtcaatttt gattcaaaag gaaaaatgac agtcggagaa gataactcga cagtccaata
    21541 cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat
    21601 cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag
    21661 tacgactgca caaaagcatt tttacggaaa attaacaggt gttttacttg ttgcattaac
    21721 gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga
    21781 aatggtcaaa aatatattag acggtatctc attggataac ttgattggac cattcttgtg
    21841 gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg
    21901 tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct
    21961 atctctaggc ggatacatgc ttggattgat actaggagct tcagatccaa acaatatcgt
    22021 tattcggatc acttcgtata ttccattctt atcttcatat attatgccga ttcgcttagc
    22081 aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac
    22141 attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tttatagcga
    22201 aggcggatta tggagttcat tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa
    22261 agcataaaac aatcctaaaa tacagaagga agctggacat ctattgttcc cagcttcttt
    22321 ttgtatagac ctattttatt tattttacta tattggatat atattcattg aaaatagtgt
    22381 ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt
    22441 tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc
    22501 tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg
    22561 aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca
    22621 aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg
    22681 cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga
    22741 tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc
    22801 gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc
    22861 gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg
    22921 aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc atcgatcatt
    22981 tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg atggatcgaa
    23041 tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg tggattggtg
    23101 cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca cgctataatt
    23161 atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt tcttttgctc
    23221 atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa
    23281 gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa ttcaaatcag
    23341 aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa
    23401 aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag ttgaaaggaa
    23461 cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag gaacaaaaag
    23521 tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa catatgacgt
    23581 ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg tttcatggct
    23641 gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa tcttatcgcg
    23701 gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa gatctgactg
    23761 caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt
    23821 tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag cctcatacgc
    23881 acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc agtgaaaaaa
    23941 ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg cacacgccag
    24001 taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt gtcattgacg
    24061 gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca ctattatgca
    24121 attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa caacaatttg
    24181 caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac
    24241 tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag ctcaaaaggc
    24301 tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc catagactgg
    24361 agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt tcttctgcca
    24421 actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt tgaaattgct
    24481 ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa
    24541 gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa
    24601 tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa gtctattaaa
    24661 attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg tgggtaagtt
    24721 ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga accgcttcaa
    24781 cgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct ttgactgcct
    24841 gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac aaactgtctg
    24901 aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta
    24961 ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa acgacattca
    25021 atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat gcttccaaga
    25081 tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt ggatgaggat
    25141 cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt ttgtcgattt
    25201 gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga ggcaatcctt
    25261 gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga tttccttgga
    25321 aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt ttcatttgta
    25381 ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact atgtaagact
    25441 tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa
    25501 attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata
    25561 aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat
    25621 tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa
    25681 agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca
    25741 aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa
    25801 gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact atcgacaaca
    25861 actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag aaaaagaaaa
    25921 aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa
    25981 gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt atggccgtca
    26041 ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag
    26101 cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga acaaacaacc
    26161 agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca aaattaagcg
    26221 ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa caccacggcg
    26281 atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa gatttggaag
    26341 ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat ttaaaagaaa
    26401 agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa tggcttcttc
    26461 attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga
    26521 tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt aagcttgatg
    26581 acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt
    26641 taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc aataaagaaa
    26701 cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag caattatcca
    26761 ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt ggctatatcc
    26821 aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa aaaataaaag
    26881 aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa
    26941 aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt
    27001 tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc
    27061 cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag
    27121 tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag
    27181 atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa ctacttgttc
    27241 taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata ccatcgatca
    27301 tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa ctgtttggca
    27361 tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat
    27421 ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca aaggaaaata
    27481 tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa gggatcctaa
    27541 ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca tttgtttacc
    27601 ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg attatccaaa
    27661 cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga ctgaccggct
    27721 atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca ggcggaagta
    27781 ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga ttcagtgtag
    27841 ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg ctaagtgcct
    27901 atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat acaggaggcg
    27961 gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa
    28021 aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt agtcaaaatg
    28081 gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca gggatcggtt
    28141 cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca agtcaacccg
    28201 aaaaattcgg gcaggctctg attctgcaat tgcttccagg cacgcaaggt ttgtatggat
    28261 tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt gtacaaggac
    28321 tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca ggtattgccc
    28381 aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa
    28441 aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc gttatttcct
    28501 tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa
    28561 aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag aacaagaaga
    28621 gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa cagaacatca
    28681 aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg
    28741 ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt taagacgatt
    28801 gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat
    28861 caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg ctggagaaaa
    28921 atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac ttccctttat
    28981 gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg
    29041 agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt
    29101 tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta
    29161 aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg
    29221 attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca
    29281 tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt
    29341 ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg
    29401 cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa tcaaaatctg
    29461 gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat
    29521 acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga agtacgtgat
    29581 tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg
    29641 acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt
    29701 gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca
    29761 tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg
    29821 aactttgtag ataaagatat ggaagattac acgaattttc tattagagac agattatgct
    29881 gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct tgaacagttg
    29941 aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg
    30001 ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt
    30061 atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag acaagtctat
    30121 gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg
    30181 ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc
    30241 aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac
    30301 gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg
    30361 atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa
    30421 tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc
    30481 cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt
    30541 gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca
    30601 agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc
    30661 tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc
    30721 attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc
    30781 cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt
    30841 gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat
    30901 ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga
    30961 tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa
    31021 atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat
    31081 cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt
    31141 acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga
    31201 tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt
    31261 aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt
    31321 attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg
    31381 aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc
    31441 cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg
    31501 tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg
    31561 aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt
    31621 atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa
    31681 agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg
    31741 gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc atatccttca
    31801 agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct
    31861 gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt
    31921 agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga
    31981 tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga
    32041 agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt
    32101 atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact
    32161 agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat
    32221 gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg
    32281 aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga
    32341 ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag
    32401 gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac
    32461 taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata
    32521 acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag
    32581 tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga
    32641 atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag
    32701 aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg
    32761 tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc
    32821 gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa
    32881 tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa
    32941 agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag
    33001 agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca
    33061 cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta
    33121 ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga
    33181 ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc
    33241 agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg
    33301 gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag
    33361 gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca
    33421 agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca
    33481 cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa
    33541 gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt
    33601 gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa
    33661 aacagttgac aactgccaca agagggcata aattattaaa agataagcaa gatgaactga
    33721 tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt
    33781 ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca
    33841 tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata
    33901 tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac
    33961 ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta
    34021 ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg
    34081 cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc
    34141 aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag
    34201 taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta
    34261 aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca
    34321 gcttctcgca ttaggttttt ttctcctgat tttagttggc ggcggcctac ttaccctacc
    34381 attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc
    34441 tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg
    34501 tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat
    34561 tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga
    34621 agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt
    34681 ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt
    34741 tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc
    34801 agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat
    34861 ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact
    34921 ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg
    34981 taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct
    35041 agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag
    35101 aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac
    35161 gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac
    35221 tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga
    35281 agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt
    35341 tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa
    35401 aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct
    35461 tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat
    35521 gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag
    35581 aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct
    35641 gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga
    35701 tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg
    35761 tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg
    35821 ttataattca tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc
    35881 aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa
    35941 gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg
    36001 gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc
    36061 tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc
    36121 taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc
    36181 agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg
    36241 aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta
    36301 gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt
    36361 tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc
    36421 ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg
    36481 cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg
    36541 cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc
    36601 aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc
    36661 agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag attctaagga
    36721 cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc
    36781 tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat
    36841 aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat
    36901 atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa
    36961 tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt
    37021 gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg
    37081 agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg
    37141 ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata
    37201 agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga
    37261 ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa
    37321 ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg
    37381 tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc
    37441 tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata
    37501 ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca
    37561 tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc
    37621 aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc
    37681 aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt
    37741 gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga
    37801 aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa
    37861 tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag
    37921 taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg
    37981 cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta
    38041 tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga
    38101 aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa
    38161 aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga
    38221 agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac
    38281 ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg
    38341 gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac
    38401 aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt
    38461 acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta
    38521 tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta
    38581 tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa
    38641 gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta
    38701 gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat
    38761 tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg
    38821 caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta
    38881 tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa
    38941 ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat
    39001 cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt
    39061 ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg
    39121 ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt
    39181 ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac
    39241 ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa
    39301 tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta atttttctag
    39361 atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc
    39421 atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt
    39481 aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata
    39541 aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga
    39601 ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt
    39661 tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg
    39721 gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac
    39781 ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc
    39841 aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc
    39901 gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg
    39961 agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata atccaggata
    40021 tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat
    40081 ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc
    40141 agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc
    40201 tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt
    40261 cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg
    40321 ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca
    40381 tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac
    40441 ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat
    40501 cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca
    40561 tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg
    40621 gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt
    40681 agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc attacttcga
    40741 tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt
    40801 gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact
    40861 cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac
    40921 cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat
    40981 ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata
    41041 tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag
    41101 tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga
    41161 aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg
    41221 ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt
    41281 gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa
    41341 caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc
    41401 ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat
    41461 catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat
    41521 catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt
    41581 gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca
    41641 gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca
    41701 tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga
    41761 tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt
    41821 ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac
    41881 tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct
    41941 tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag
    42001 tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag
    42061 agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct
    42121 gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc
    42181 agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc
    42241 cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt
    42301 tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta tggactggat
    42361 cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc
    42421 tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg
    42481 atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca
    42541 aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct
    42601 tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct
    42661 tttaaagcag attgataaaa tcgcttcatg acttgcccac gtttttcctg attaccgttg
    42721 acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca
    42781 aattttttgc catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg
    42841 aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa
    42901 gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa
    42961 attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct
    43021 gcatcttcca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct
    43081 aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa
    43141 tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc
    43201 tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct
    43261 acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg
    43321 acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag
    43381 aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa
    43441 aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg
    43501 gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg
    43561 taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga
    43621 tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta
    43681 tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag
    43741 cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt
    43801 atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat
    43861 tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg
    43921 aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac
    43981 gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat
    44041 cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca
    44101 gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga
    44161 tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta
    44221 aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg
    44281 atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg
    44341 atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg
    44401 acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag
    44461 acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat
    44521 aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg
    44581 tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata
    44641 aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa
    44701 ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca
    44761 cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta
    44821 cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa
    44881 ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat
    44941 ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat
    45001 tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt
    45061 agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg
    45121 tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta
    45181 ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt
    45241 cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag
    45301 ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa
    45361 agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa
    45421 caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag
    45481 gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata
    45541 ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa
    45601 atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa
    45661 attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc
    45721 ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa
    45781 gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag
    45841 atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca
    45901 aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa
    45961 aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt
    46021 tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc
    46081 ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa
    46141 gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa
    46201 aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc
    46261 cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca
    46321 aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa
    46381 aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt
    46441 acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt
    46501 caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct
    46561 gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc
    46621 gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac
    46681 atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa
    46741 agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta
    46801 tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt
    46861 tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac
    46921 agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt
    46981 tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt
    47041 attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca
    47101 gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg aacacataac
    47161 taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa
    47221 ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct
    47281 aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt
    47341 acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta aacagataaa
    47401 aaaatacaag gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc
    47461 agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat
    47521 ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg
    47581 aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag
    47641 gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat
    47701 tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc
    47761 ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag
    47821 agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac
    47881 cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg
    47941 attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc
    48001 gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat
    48061 tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat
    48121 tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct
    48181 caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac
    48241 aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt
    48301 tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt
    48361 cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg
    48421 aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat
    48481 atttggaaac agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta
    48541 cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg
    48601 aattagtcga tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc
    48661 tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag
    48721 gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa
    48781 aagtatttca ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta
    48841 cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt
    48901 ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc
    48961 gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg
    49021 caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt
    49081 ttatcggaaa gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa
    49141 aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa
    49201 gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa
    49261 cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag
    49321 cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt
    49381 ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa
    49441 caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg
    49501 attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca
    49561 aacggtaacc accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct
    49621 ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt
    49681 attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa
    49741 ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa
    49801 aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa
    49861 gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat
    49921 atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt
    49981 ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta
    50041 aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg
    50101 tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat
    50161 gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt
    50221 tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc
    50281 agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt
    50341 cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc
    50401 tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac
    50461 tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc
    50521 taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta
    50581 cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg
    50641 cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt
    50701 aattcctgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg
    50761 ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc
    50821 agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc
    50881 agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac
    50941 agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac
    51001 tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa
    51061 gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt
    51121 tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg
    51181 atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg
    51241 tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac
    51301 aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag
    51361 aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa
    51421 cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg
    51481 gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca
    51541 aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg
    51601 ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc
    51661 caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag
    51721 aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg
    51781 taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca
    51841 cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg
    51901 aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg
    51961 tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg
    52021 atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct gatacattac
    52081 aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg
    52141 ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca
    52201 tcggtggcgg agactcagct gcagcagcag aacaattagg atttgctgac aaattcacac
    52261 acatctcaac aggtggtggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac
    52321 tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc
    52381 aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta aggagtgtgt
    52441 tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa
    52501 gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca
    52561 gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa
    52621 ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact
    52681 tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt
    52741 cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcaaaagc tatctttgca
    52801 aatggtatga caccaatcct atgctgcggc gaatctttag aaacctatga agcaggcaaa
    52861 actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa
    52921 gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct
    52981 gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat
    53041 ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat
    53101 attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg
    53161 caagcagatt catttttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa
    53221 aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat
    53281 cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta
    53341 cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga
    53401 aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc
    53461 agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt
    53521 cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat
    53581 ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc
    53641 gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt
    53701 acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa
    53761 catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat
    53821 gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc
    53881 attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt
    53941 tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa
    54001 agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt
    54061 ctacgacaaa gaaaaaggtg tttacgtact agctgactca ggcgaaggcg aaaaaacaac
    54121 tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga
    54181 agatggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga
    54241 caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg
    54301 tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac
    54361 agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca
    54421 ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaatgctgg
    54481 ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact
    54541 acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa
    54601 cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg aaagatagtc
    54661 gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga
    54721 gagttttttg gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgagtca
    54781 gaatgaacca cattctggct cagatttttt gttatcttac attgattaat tgattgatca
    54841 aaaagattct aattctcatg ttctcaaatg atttaaatcc gtaggatact cgtttcattg
    54901 tctttatgtg ggtattcttc gcttctattt ttccattgga ataaggatag atcattgcgt
    54961 tggtgatgcc ttcttcatag gtcagaaggt tttgaagctt ttcccgaaag ctgtcatcta
    55021 acgtttcggg aagttctgcc aataaggaga aaaataagtc agggtctttg tctcgaaagg
    55081 cttcaactaa ttcatgaaaa aagggatacg cctcctttag gggcggagaa aactcaagca
    55141 atcgatcaat catcattgct tcagtaagaa atgggtattt tggtgctcgg aaacttttcc
    55201 atgttttgta ttcataatgg ttgatgtttg cacgattttt tagcaaaaag cgccagttct
    55261 ttttcagttt ttctgcctgg cttttctgtc cggctttacg aagttcattc atttcacgga
    55321 tacgcaactc attgaacgct tgattcatgt gtttgacaat atgaaaccga tcaatcacga
    55381 ctttcgcatt tggcagaaca cgtttggtga gctggaagta ggcggcgttc atgtctgtca
    55441 ccaagaattc tacttcttct ggattggtac aacctaagaa atagcttgtt aatcgaggta
    55501 atttacgcgt aggcaaaaca tctattaatt ttcctgtttc gccatccgcg caaataaagc
    55561 tcatcttatc ttctatggaa gcatgcgaac gaaattcgtc aaccatcaat actctgggga
    55621 ggatcttctt agattgcttt ggtaaatagc ttttaaactc tttcaatgta cgaataacgg
    55681 tggtcaaaga tacctgacag cttttcgcaa taaaagataa agatactttt tcagtcagta
    55741 aagaagcaat tttatatcta acatgatttg cgattgaatg tctgggttgg acaaaataac
    55801 tttgagccgt ccaatgggtg cgacagtttt tacaggtata gcgttgcttt tttaggcgca
    55861 taaccaaagg catatgattg tattgttcaa aacggacaat cgtttccttt tttccatttt
    55921 tcactataat tgctttcccg tttccatcta ccacagtaga accacaactt ctacaagcac
    55981 gaggagtagg cgagagaaca gcatcgacga ccaacgtctt tttcttctga agggtctcgt
    56041 aagagacctc tgtaatcatc aaatctttct ctgttattct cagcattttt ttgatagaat
    56101 cattcatata gcgtatcgtc ctctcagttg tttattttgt ggtgatttaa tcatactaga
    56161 gaacgatatg ttttttaata cctaaaat
//
LOCUS       DACCQP010000012        55720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCQP010000012 DACCQP010000000
VERSION     DACCQP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.012"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IVO87_000801"
     CDS             1..369
                     /locus_tag="IVO87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7449019.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IVO87_000802"
     CDS             549..1490
                     /locus_tag="IVO87_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7449020.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IVO87_000803"
     CDS             1559..2290
                     /locus_tag="IVO87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7449021.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IVO87_000804"
     CDS             2434..4614
                     /locus_tag="IVO87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7449022.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="IVO87_000805"
     CDS             4634..5116
                     /locus_tag="IVO87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7449023.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="IVO87_000806"
     CDS             5132..5893
                     /locus_tag="IVO87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7449024.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="IVO87_000807"
     CDS             6024..7358
                     /locus_tag="IVO87_000807"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7449025.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="IVO87_000808"
     CDS             7665..8150
                     /locus_tag="IVO87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7449026.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="IVO87_000809"
     CDS             8143..8826
                     /locus_tag="IVO87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7449027.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="IVO87_000810"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="IVO87_000810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7449028.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="IVO87_000811"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="IVO87_000811"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7449029.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="IVO87_000812"
     CDS             12442..13071
                     /locus_tag="IVO87_000812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7449030.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="IVO87_000813"
     CDS             13123..13455
                     /locus_tag="IVO87_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7449031.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="IVO87_000814"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="IVO87_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7449032.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="IVO87_000815"
     CDS             14281..15654
                     /locus_tag="IVO87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7449033.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="IVO87_000816"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="IVO87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7449034.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="IVO87_000817"
     CDS             16709..17149
                     /locus_tag="IVO87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7449035.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="IVO87_000818"
     CDS             17293..17994
                     /locus_tag="IVO87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7449036.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="IVO87_000819"
     CDS             17991..19112
                     /locus_tag="IVO87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7449037.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="IVO87_000820"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="IVO87_000820"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7449038.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="IVO87_000821"
     CDS             20491..21399
                     /locus_tag="IVO87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7449039.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="IVO87_000822"
     CDS             21434..21706
                     /locus_tag="IVO87_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7449040.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="IVO87_000823"
     CDS             21717..22763
                     /locus_tag="IVO87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7449041.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="IVO87_000824"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="IVO87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7449042.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26445)
                     /locus_tag="IVO87_000825"
     CDS             complement(25774..26445)
                     /locus_tag="IVO87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449043.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26438..26932)
                     /locus_tag="IVO87_000826"
     CDS             complement(26438..26932)
                     /locus_tag="IVO87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7449044.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="IVO87_000827"
     CDS             complement(26952..28172)
                     /locus_tag="IVO87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7449045.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="IVO87_000828"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="IVO87_000828"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7449046.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30350..31261
                     /locus_tag="IVO87_000829"
     CDS             30350..31261
                     /locus_tag="IVO87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449047.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32257..32508
                     /locus_tag="IVO87_000830"
     CDS             32257..32508
                     /locus_tag="IVO87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449048.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32591..32863
                     /locus_tag="IVO87_000831"
     CDS             32591..32863
                     /locus_tag="IVO87_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449049.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            32953..33192
                     /locus_tag="IVO87_000832"
     CDS             32953..33192
                     /locus_tag="IVO87_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449050.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33339..37622
                     /locus_tag="IVO87_000833"
     CDS             33339..37622
                     /locus_tag="IVO87_000833"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP7449051.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37721..38086
                     /locus_tag="IVO87_000834"
     CDS             37721..38086
                     /locus_tag="IVO87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449052.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38088..38540
                     /locus_tag="IVO87_000835"
     CDS             38088..38540
                     /locus_tag="IVO87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449053.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            39154..40464
                     /locus_tag="IVO87_000836"
     CDS             39154..40464
                     /locus_tag="IVO87_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7449054.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40487..40798
                     /locus_tag="IVO87_000837"
     CDS             40487..40798
                     /locus_tag="IVO87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449055.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41159..42415
                     /locus_tag="IVO87_000838"
     CDS             41159..42415
                     /locus_tag="IVO87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7449056.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42412..42957
                     /locus_tag="IVO87_000839"
     CDS             42412..42957
                     /locus_tag="IVO87_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449057.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42958..43458
                     /locus_tag="IVO87_000840"
     CDS             42958..43458
                     /locus_tag="IVO87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449058.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43539..44095
                     /locus_tag="IVO87_000841"
                     /pseudo
     CDS             43539..44095
                     /locus_tag="IVO87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44115..44459
                     /locus_tag="IVO87_000842"
     CDS             44115..44459
                     /locus_tag="IVO87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449059.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44478..45389
                     /locus_tag="IVO87_000843"
     CDS             44478..45389
                     /locus_tag="IVO87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7449060.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45468..46460
                     /locus_tag="IVO87_000844"
     CDS             45468..46460
                     /locus_tag="IVO87_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7449061.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46457..46675
                     /locus_tag="IVO87_000845"
     CDS             46457..46675
                     /locus_tag="IVO87_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449062.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46688..47098
                     /locus_tag="IVO87_000846"
     CDS             46688..47098
                     /locus_tag="IVO87_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449063.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47150..49678
                     /locus_tag="IVO87_000847"
     CDS             47150..49678
                     /locus_tag="IVO87_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7449064.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49694..51904
                     /locus_tag="IVO87_000848"
     CDS             49694..51904
                     /locus_tag="IVO87_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449065.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51912..53003
                     /locus_tag="IVO87_000849"
     CDS             51912..53003
                     /locus_tag="IVO87_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7449066.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53024..53566
                     /locus_tag="IVO87_000850"
     CDS             53024..53566
                     /locus_tag="IVO87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449067.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            53788..54018
                     /locus_tag="IVO87_000851"
     CDS             53788..54018
                     /locus_tag="IVO87_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449068.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54032..54364
                     /locus_tag="IVO87_000852"
     CDS             54032..54364
                     /locus_tag="IVO87_000852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449069.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54409..54957
                     /locus_tag="IVO87_000853"
     CDS             54409..54957
                     /locus_tag="IVO87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7449070.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54964..55416
                     /locus_tag="IVO87_000854"
     CDS             54964..55416
                     /locus_tag="IVO87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449071.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            55513..>55720
                     /locus_tag="IVO87_000855"
     CDS             55513..>55720
                     /locus_tag="IVO87_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449072.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT        18942 a         9036 c        11972 g        15770 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta
    30181 aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga ttttaaactg
    30241 tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt cttgaagatt
    30301 tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa
    30361 ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt
    30421 agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa
    30481 aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa ctctaataaa
    30541 agatgaggaa gataagattg agattctcat cggtaaacta attgcataca gagatatgaa
    30601 taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta
    30661 tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct
    30721 attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga tatttagttt
    30781 atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga
    30841 agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag
    30901 tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct
    30961 tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata tacttccaaa
    31021 agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat taaggatgag
    31081 gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc
    31141 aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca atactaatcg
    31201 aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt acgggatata
    31261 aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattatag aaaaaattta
    31321 taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag ttgctttact
    31381 ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc ggaagaagta
    31441 atacaagaac taactgttat gaataacagc tagataaaat gggctatgta tgttatccaa
    31501 caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata gatgagtatt
    31561 gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt tgtcctctgc
    31621 agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga atacttacgt
    31681 ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat tgactgaatt
    31741 cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa ttggggatct
    31801 tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa tttctctaaa
    31861 aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac tgatatagaa
    31921 taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc cgcaaattat
    31981 atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag aaaaagttgg
    32041 ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa atttgtgtta
    32101 ttaggttagg acaaagataa gaagaagtat gaagatttaa aaaaggaatg aggaaaagat
    32161 agtaatttcc gattattgga gtttttttat ttataaagaa gaagcgaata agttgtgatt
    32221 cctagatttc gcgcgtgatt aattaatgag caaaaaatgc ggaaagtttt agtgtttaat
    32281 acacgaggac aaagtttctt aggtattgaa ggcacacgtg taacggttga tgggtggtgt
    32341 gattgtttcg aggttggaaa acatgttgaa cttatggcat ttactgagct aaaagataag
    32401 aacaacgtgg aatttttcga gagtgatatt ggttgggacg atcatgaaga agtttatgga
    32461 aaatttattt tgcggaaggg aaattcttat acgaatgggg aagcataagt agcgatttgt
    32521 tagagatcat tgataggatt gaaattgtcg ggaatattta tgagaatcta gagttatttg
    32581 aggtgataga atgagtgatg gttttgaaca agtatcaata attaaaaatc aagttcggga
    32641 gattcttaga aaaaaaggct atttggtaga tagttatttt gaaggagatt acgaaacatg
    32701 ggtaggtgtc tatgctcgtc cagagaataa acctacctat ttagatccta cgacgtcaga
    32761 agatgcctat ttacaaaata ggtatcgagt agatggtttt aagcaagact ttgcggagtg
    32821 gtttgaatgg gaaattgaaa acggagaagt aaaagaagaa taagagtact tgttgtatca
    32881 aaataagcca gtagaacaac caaaccagta gctagtaatg acaataatac tgaggaatcg
    32941 gatcattatg gtatgattga tatttctctt ccagaagatc ttaagccaga tcctgtaaca
    33001 attggcaata gtgtcatgat ttttgattca ttagatgaag ctatgtcata tggttggaaa
    33061 gaaattaagg atgaaggtag taattggtat ggcatgagat ttggtgatgg tgaagttgcc
    33121 aaaaaagctg gtattccaat tgggaaatgg acagttgatt tccacgaagc aggttctttt
    33181 tttgttctgt aattatagtg ggagggtaaa atcattgatg aaatgtgttc agataagagc
    33241 gccagataaa aatagaagag acagttcacg tcgttatcac ttttggtaac ggcgttttgt
    33301 tttattatat aaaattctca ggaaaaggag gaattcttgt gtatgcaaaa acgaaaatga
    33361 attggttgaa agcactcgca ttggtggctt tttctcaatt ctgtttcttg gtgggaactg
    33421 ctcaagcaca tgcagaagaa acgacggcta tacaacctac gatggaagcc attgcccaag
    33481 tcgcttctga agtgacagaa gtccaaccag cagaagccgg ggtcccagct ggtgaagcca
    33541 ctgttgaaca ggcccctgtg acagaagcgc caacggtcaa ttccaaaacg actctcgatc
    33601 agacgattgg ggaaacacaa cacccaggga atagtcctgg tacttcacaa ccagttgaag
    33661 aaactgcctc aattacaacc cctgcttcag aaacgtccac tctagaagga gaaaaagctg
    33721 ccgaagcaac gccttctgtg cagccagagt cttctgagaa acaagcggaa gaaaagaaac
    33781 gaatagaaga aacagtggaa caaccagcgg ctactgctga caaagaagga aaaaatgagg
    33841 aaaacgttga aaaaacaatc tcttcaaccg cagctagtca aaatcaacca acgaaaccaa
    33901 acaaagaatc tacggctagt attgaaaatc aaaagaaaaa agagcagaac attccaaaaa
    33961 caggtacacc tacactgtta gtaacagtac caaccaatcg ttcggttcgt gttcgccgtg
    34021 caaccaatgc agctgtttta ggtggtcgga aaattgattg gggcgaaaat attggtgtta
    34081 cttctagtcc gaacaatcta aacttcactt taaattatat ggttcgtttt gagattaatg
    34141 gacaagtggc gttttgtatt gagcctggtg tctcagccgc agaaggtcaa gtttatacag
    34201 ctactgcttt gaaaaactat ttgaaaaatt caaatgtacg gaaaaaaaca agcttgatct
    34261 cttatttagg atatgtcaca aataaagaca aatccgatga acaatacatt gcggcacaat
    34321 ttatgatttg ggaagctttg ggcacaaaaa tcccaatcaa aaatatccgt gttgattatg
    34381 atcgtcgaaa agcagaaatt caacgtttga ttaataactt tggtgaaaaa gcaactttta
    34441 ataatcaaac acatacggta aaaattggcg aaacgttgaa agtaaagaat aaaaatgatt
    34501 ttttgaaata tgtgaaagac attcaaaaac caaaaggtgt caatgtatct attcacggca
    34561 atgaagtatg gattacagtc acaaaagagg caccggataa agcaaaaatt caattgaata
    34621 aaattaaagt tcaaggtgcg ccattagcct atcgtcgtcc aggagctcaa acaattggtg
    34681 tgttacatcc aacagaacct aatgcgtctg ttattaattt acaaattcta aaacaaggaa
    34741 acattcgtgt gtttaaagaa gatgcggaaa ctggtgtgaa agcacaaggt gcagcttctt
    34801 tagaaaatgc ggtctatggt ttgtatcaag cagatgggaa aaagattaaa gaaattacct
    34861 tgaaaaaaat taacggcaaa gtacaggcag aaattaaaga tcttgatcct ggcaaatatg
    34921 ttttaaaaga aatcaaagcg ccaaaaggtt atgtgctttc tgatgaagca attgaaattg
    34981 ttgttgaacc tggaaaaact gtcaatgcga ccgcaaaaga tcaagtgatc aaaggaacca
    35041 ttgatttagt gaaagttgct aataaagatt tagtcgattc aacgaatcca gataacaaac
    35101 caaaattagc tggcattgaa atcactttga cgtcaaaaac aactggcaaa gtggtgaaaa
    35161 ccgttgtgac agacaatgat ggttatgcta cttttggtaa aaataccgtt gtctttgata
    35221 cgtacattct ttctgaaacc aaaggaaaag aagggtataa actatttgaa ccacttgaag
    35281 tcacaatttc tgaaaatggg caaactttcc attatgtttt agaagataaa gtaattgaac
    35341 aacgtcttaa agtggtgaaa gtggatcaag aaacaggaaa agtgattcca cttgctaata
    35401 cagaattcaa gatttttgat actttagcca atcgttatgt cacaatggag attccaaatg
    35461 atacagaaac aacggatatc tttaaaacca atgaaaaagg ctttttccta actaacggta
    35521 cacttactta tggaaaagat cgctatcgtt tggaagaaat caaagccccc gtaaattacg
    35581 ttttgaatca aaacccatta gtgttctcga ttaccaatga tacagaaccg atcaaagtga
    35641 tccattttgc gaaccgtcaa gcacggaaaa gtgtaaaatt attcaagtat gaagaatgga
    35701 acaagaagaa aacgccgctc gcaggtgtga actttacctt gtataacggt aaaaatcaag
    35761 caattggtga atataccact gatcaaaacg gggaaatttt agtgaaaggt ctagttgccg
    35821 atgactatta cttcttggaa aatgccccat tagaaagttt ccaaccaaat acagataaac
    35881 aagcattcac ggttgcattc aaacaaaatg cgccagatga tggggaaacg ttagtggttt
    35941 ctgtacgtaa ttacttgatt ccaccaaaac tagaaacaca agcgtcaaat aaagccgatg
    36001 gtgaaaaagt ggttgatcca ttagaaacga tcgtcattaa agacagtgtt tcttatacca
    36061 acttgtttgt cggaaaagaa tacaccattt caggagtatt gatgaacaag gcaacgaatg
    36121 cgccaatttt acaagatggc aaagaaatca caagtacctt ggcctttatt gcagacaaag
    36181 tcaatggcac aaaagacctt gaatttgtgg ttaatgctag tgttctgaaa ggtcaaacga
    36241 tcgttgtttt tgaaaaactt ttccgtgatg gcattgaagt tgcagcacat gcggatatca
    36301 acgatgaagc ccaaagtgtg aaagtgaata atccaaccgt tcataccacg gctgtctttg
    36361 aagacggatt gacagtcgca gacccaacag gattgtttac cttaaacgat accgttgaaa
    36421 ttaccgagat tcttccagga aaagcatact tggtcaaagg gatcttaatg gataaagcaa
    36481 ccggtgaacc cttgcttgtg catggaaata cggtagaaac gagtgttggc tttattgcca
    36541 aagcagaaaa tgaaacggtg atcgtaccat ttacctttga tttgacaggt ataaatggtc
    36601 gtgaaatcgt cgtgtttgaa agtcttttta gaaatgatga agaactagca aaccataaag
    36661 acatcaacga caaagggcaa acggtacgag taactaaccc aagtgtacgt acaacggcta
    36721 cacataaacc cactggcttg aaagaggtta atccattaac gaaagtcacc attacagacg
    36781 ctgtgaaata ccacgatttg gtcgttggaa aaacatacac agtcaacggt gttctaatgg
    36841 acaaagtaac ggaaaaacca gtgctagtag atggaaaaga agtcacaagt accttaacgt
    36901 ttgtggcaga aacaacggat ggtacagtca atctagactt tattctagac gcacgtgcgt
    36961 tgcgaggcaa agaagtcgtg gtctttgaag atctgtttag agaaggcgta ttattggctt
    37021 ctcacgcaga tattaccgac aatgaccaaa ccatcaaaat cagtaatcca accattacga
    37081 cgaaagcgac gaataaaaat ggcggaaaag agatcttggc cttgccaaat caaactgtgc
    37141 ttgaatgggt caaagtagac ggtcttgcga ttggtcaaat ctatcgctta attgttcaag
    37201 gctacttaac accagacggc acaccatttg aaggcactca tgcggaaaaa gtctttgcgg
    37261 ccgataaaga aatgatggaa ttcttatttg aattcttagt ggacggtcgc aatcttgcag
    37321 gaaaaggctt gagttttgcg gaatggctcc aagcgaaaac ggaagatgag aaagaaacgt
    37381 ttgaagaagt ggcgaaacac aatgtagatc taacaaacaa agatcaaacg gttcaaattg
    37441 tggaagcgcc aaaaccaccg caaccagtga aaccagaaca acgggttaaa ccagctactg
    37501 ttgtaccaat gttgccaaaa acaggtgaac aaacaagtag tatacttatg gttcttggat
    37561 tagtagtatt agtcattgct ttaggtataa cactttataa agtacgtaaa gccaaagatt
    37621 agtcatgagg aacaagcggt taggattttt aaccgcttgt ttgattgtaa tgtagaaata
    37681 tttagaattt attttggttt ttaagaggag gaaaaacttt atggcagaaa aagcattgcg
    37741 tatgaagaaa gagtttttag gtcaagtatt tttattgggt tcacgggaac ctaatccacc
    37801 aaaggaaatt aatggtgtgt ctgggcgata tgttcaatta caagcagagt gttcgcgaga
    37861 agaacagcct gctttttttg tttatctcac agctgaagag gataaaagat tacgattacc
    37921 agaaaaagag gtgcgtattt catttgaaga agcttatttt gtttttcgta aagaacgagt
    37981 gaatagtcgt gaaacaaatg atatgggaca aacagagcgt cgaacaactg gctatcaaga
    38041 tgttggtgtc tttttaactg gtgtaaaaat tggggaggtt ttgtaagatg ggattaaaat
    38101 ttccaaataa ttatgacatt acagaagcat tagatgaaac ttgtttagga gccgtacgct
    38161 ttggcgcatt agaagagtct gatcgtgtat ttgataatga aacaggcgat tatacgggag
    38221 aaattcgatc acagcgagca caattagttt ttggaaatcc agatgaacca aagattgcac
    38281 gacgagttaa atttccagca gatgtagatt tgactggttt taaacgacgt gatcctgtga
    38341 aattaattga tccagaatat catgtggtat atattccagc acaagatggc aatcgtgaat
    38401 attcagattt agtgatttat gcgaaaggat tagagaaagt agaagctcca acaaataatc
    38461 ctaatatgaa acaatcacaa gaaaaaggtt tagatcaaaa acaaccaagt gttgattcta
    38521 aggaaaaaga gaaaaaataa ggtttttttg aaagaatagg aaggagtctt cctattcttt
    38581 ttatctaaga tagaaggaag gggagatttt gaattcagat aaaaccaatc aaatactttg
    38641 agagattgtg cttgttatca aaatatccaa caattgattg agtattttgg agacaatcca
    38701 acgatttttg tacaaacaaa tgcatatatt tgtgcggtta ttgggcagtg tatgttttta
    38761 cgtgatcttg ttcgtttttc attaccgtac cagtggaaac aagatcatca ggaagtgatt
    38821 tggattcgta tagtacgaat atgtaatatg tgtgcacacc cttataaaaa tcgattaaat
    38881 ctttttcgat tatgtttttt tactaaatgt gatattcctg tattagttgc tttttgtcaa
    38941 aagtttttag cacaggaaaa gtaaaaacag aaaggaggaa tgtgacaatt gaaagctcat
    39001 tttttcaagt atcgtggtcg gcgaattcgc tatttcaatc gttatttatg gttttgctat
    39061 tatggtttct ttttgtttcc atttttgatt ctgttaggtt ggtttagttg gaaagtattt
    39121 tatccacgtt taagtacgtt ttcgaattgg caaatgtatc ttattcctgg aataagtatt
    39181 gtgttgtatt tgttgctggt tagtgggtta attttagggt tgatgcattg gtcgcgacta
    39241 aaagaaggat attttgcctc tgtttattgg cgtcagttac ttgttcgaat gttaattgac
    39301 aatcggtttg tttatacaaa gaaacaagtt agtgagaaac aaacaaggga aaaaatgaga
    39361 ttaccaaaag tttattttta tcaaaagaaa caagaattag tggtttcttt tcctttagat
    39421 ggtgggcgtt ttcacgaacg atttttaaaa atgggtcatt tgttatctga agtatttttg
    39481 cgtgatttaa ttcgtgaaga acgagaaaaa gcccgaatca attatgtatt cctttcagat
    39541 tctggtcgga ttcctattga tcaatgtata gcagaaaatg gacgtattca tttaatgaaa
    39601 actttggatt gggtatatga tcaatcacca aatatgttga ttgctggtgc aatcggtggt
    39661 gggaaaacct atttattgta ttctttaatt caagcttgtt taagtgttgg gacagtagat
    39721 atttgcgatg gaaaagcggc agatttagca gcattaggtg acgtcgatgt attcaaagga
    39781 catgtgtttt acaaaataaa tgaagaaatg attatttgtt tacgaaatgc attaaaagaa
    39841 atgaatcaac ggtatgccta tatgaaaacc atgaaagatc ctaaatatga accagggaaa
    39901 aattatgctt attatgggtt accaccgcat tttatctttt ttgatgaatg ggcagctttt
    39961 tatggttcgt tggatatgga agcgagaaat agagtcgata aattgattca gccattagta
    40021 ttaaagggtc gtcaagcagg gatgtacatt attttagcaa tgcaacgacc agatgcagaa
    40081 tattttccaa gtggtgttcg ggataattta acattccgta tttctgttgg tcgattgtct
    40141 cccattggtt atttaatgac ttttggagaa gattttaaaa ataagccatt ttacaacaaa
    40201 gatcaacctg gtcgtggata tgcttttcca ggatttgatg tacctgtaga gttttttgcg
    40261 ccatttgttt acaaagatta tcactttatc aaagaatttg agaaatttac tgaaatgatt
    40321 gtctatgatt ttacagtaaa agaggatagt caagatcaaa cgatgggaga tgataatcag
    40381 ttgttacaag aacaactaga gcgccaaatg tatttagcaa aagatccaaa aggaacaagt
    40441 ttttctgatg cgcatatttt gtaatcaact aaggaggtga agttaaatgg cagagagttg
    40501 ttattataga gtagctacag caatacgaat gattaatcct tctctttctt ccagaacgtt
    40561 ttatgattgg ttgaatcgta ttgaacaagt aactgattac cgctttttgc gcaaagaacg
    40621 tgtttttaca ggaaaagtta ttaatcaagt attgctaacg aaaaaagata tagaacgatt
    40681 gactcgattg tatcattatc gagtagattt agaagaggat ttaactttgt caatttatcg
    40741 cgttttttct cctgaaaaat atagtgaaat aacaaaatta gatcatttaa ttttgtagtt
    40801 attatctatc gggagggctc tagacgtagt ctggtagccg cactctgcct ggcggtgcgc
    40861 attaaggctg cgaagcatgg gcctataatg cgcactcgct agcagcgtgt gcagctcaca
    40921 aagaggtcgt gcgcttgcgc acggcctctt tgtgtaaagc cccccgatat ataatatggg
    40981 gggcagaaat aacataacac atagaaaaaa gttttataaa cccttttgta tcaagtgatt
    41041 atctaaggtc agcagttgta ctacttttcc caaaaaacga gggggactag ttgatatatc
    41101 ggtgtttcta aagggttaaa aaaaatcgat tttgttattt tataaaagga gcgagaaaat
    41161 gacaccgatt gagctaaaaa gctacagaaa gaagttaagt gcaattcata atataagtgg
    41221 agataaaaag ccgctatcac aagcaagatt tgcgcaattg atgggctgct caaaagatta
    41281 cattagaaag atggaacaag ggaaaagaaa aatcccagag gattttgaac aacgaatcaa
    41341 ggaacgcttt cgattgtata tgaaaagcgg aatagatctt cgagcaatga ttgattattt
    41401 gcgtctttcc ttttttggga cagactacaa aacagtgatt caacgtgtat tacgaatgga
    41461 tctgacagaa tttgaaacac gtccaacggg cttgtatctt tttgactgtc tttctgttcg
    41521 tgggaatatc tgggtgttct ggcattcaaa acgaactgta acagaaaatg tacttgtgca
    41581 attttcagga caaggttgcc gggaatatga ggaagtcatg cgtgaacaac aaacagattg
    41641 gcgaacatgg ctatattcca tttggaaaaa tcaaggaaag ttggaagctg gttatacacg
    41701 tgtgcaatgt agccgattcg atattgcgtt agatgaactt tggggattgg aaacggaaga
    41761 acatttcgat ctctttgaat tactagaaaa acaaaaagca ggtttattag aaatggactt
    41821 taagagattc cagaatattg gcggttgttt tttagatgat ggctattttc gaagtgaagg
    41881 attatctttg tattttggat cgcgaaagag tcctctcttt tttaattttt atgagaaacg
    41941 ttttgaaatt gcgaaccgag aaaaaatttc agaattagaa gcattaacga aatatggtat
    42001 ttataatcga tatgagttac ggttagccaa tgagaaagca acacaagcag tagaagcatt
    42061 tattttaggt gattcaccga aacgtttagg agagattggc gttggtttaa ttaatgcaaa
    42121 acttattgtt tgggatagac tggaaagtga tgcacggatt gttgataaac gatgggcaaa
    42181 attatttcgt tcgatggaag aattggattt tacagtaaag ccagaaaaat tctcattaga
    42241 tcgttcagaa cgctggtttt tgaaacaagt agctccgaca ttaaagttgt tacaagaagc
    42301 agatcgtatt ttaggaagag actttgtaca agaagggatt gataatgccg aactaaatga
    42361 cgagcaacaa aactatctag aatggatggg aacgtcagga gggaaaattg catgaatgca
    42421 tatacgacaa cagaatggct gcgcctgttg gatttaaagg ctgcaatcga agcattaaat
    42481 gaaaagatgg ttgatttgtc ttattttcgt tttcgagttc cttatattga acaagcggta
    42541 aaagctggac gttatcagga aaaagagaac tggcaagaaa ttgcacgctt attagaagtt
    42601 cgtaagggat acgaacaaga actcgaagag ttggaattta gccgaaggaa aggaaggcta
    42661 gaattcattc gcttttatcg attttcattg ccaatacctg cgattttagc ggttaaaaaa
    42721 ggctgcgata aaatgaagat atatgaaaac tgtgtggcag cacttagcag cgaaaaacct
    42781 cttatggaag aaatttcatt ggtgactgtc acttgggaaa tgagtcgcca gccaacagaa
    42841 gaacagacat atctttcttt agaagagatt gagatagaat tagaggaaat tggtcgctat
    42901 gccacttgtt ctacctattg tggatcggtc attagtattg ctggtgtgat tgtctaaatg
    42961 aaaaaaatta tgatttatgt tggtgcgtat tggtcacgtg atccaacagt attagaaaat
    43021 ccagaggcgg tacactattg cttgcgccaa ttattctatc tgtataaaga acgattagaa
    43081 tcgttgattc gtcagttacc atatactgac agacgtttag atgaacttct tctaaggtat
    43141 ccagcaatgt ataaacgacg aaaaaataga ttgttaccag aagagtatcc cattgaaaaa
    43201 catgaacttg aagggcgatt tgtggcttat ttctatgatg atgttcgtat gcgcttagta
    43261 gaacaacgaa tggaaataga aaatgatcgc tactatttta tcaattattg taagaagcgt
    43321 aagtatcaag taactgatga tttttatgat tgtattttac aagatgggga agtgattcta
    43381 tccaaaattg ctccaagttt tcgtgaactt gtgccgattg attttttgaa aaaatgtcat
    43441 ttgcggattc ttccgtaaaa aagagctata aaaggaaaga aaagtagaag ccaaatcggt
    43501 ttctgctttt ttattttttt taggaaggag aagatggtat gtcactggaa tttttaggag
    43561 agccaattga aaaggaagag ttcgtttcgc attatatgga tttgtttatt caggtgataa
    43621 aaaactttgt gcgattgatg aattttatcc acgcgaacaa gactatctag ccgctgaacg
    43681 aataaaaatc agtttattat gggaaaaatt cacagatgaa aataaccgac cgccagatta
    43741 tcgctattta tcagattgtt taacgaataa tgtgctttct aaagaatacc tatttcacaa
    43801 acgtgagaaa aatcaaatgt cggttgaaca tttttgtgat tgttatattg cattattgcg
    43861 tagtcaacag ttattaacag tagaacgata tggtgcagag tttggttatg tcatgcaagc
    43921 tgagcgtcat agagctacaa gatgctatga aaaagaagat caagtgattg atggctatga
    43981 agaattatgt attcgaaaca atcgctattt gcaacaacgt ttatatgaac aattgatgat
    44041 ggatttttca aatctctata gagggtatga atgttcgatg attcaagaaa ggtagaagaa
    44101 aagggggaat ttttatgtgg cgattattat ttttggtggt atgtggtatg gcattgttct
    44161 atgtgtatcg tgtctatctc tatccacgaa tggaacgatg gacaagttgg tatagagatc
    44221 ctttaaaaca acgaaaagga ttgaagcggt atcgttatga attacttgtt gaattttcgg
    44281 atgggcgaca agcacagtat tatttttttg caaactatcc aaaatacaac accagtgaac
    44341 tattgattca attattcaaa aataatccat atgttgggtt agaagaaaac ggacgggagt
    44401 tcttttatcg ttcagataat attagtgcgg cgtctatgaa caaaacacga attgcataaa
    44461 aaattggagg aataatcatg aaaaaaacaa cattagcagt cgtaacaaca ctaggaattt
    44521 cattattttc gtttcaatta gggagttcat tggtttatgc agatgaaact gtagaaacac
    44581 ctaagtcaga aattgtagaa acattgccag gggaaggatt accagatgtt gagacagagc
    44641 taaatggagg aacaacggaa aaaccagcag aacctgaaca attgcagtca gagaaacctg
    44701 aaaaagagcc agtgacacca gaaccagagt ttcctgaatc aactttaccg actgattcga
    44761 tcgaagaaga aggtgtgcat agttcaacaa caacggattc aagtatagtc acaactccgg
    44821 aacaatcagg aacaaccagt tcttctgaaa agaatactga ttcgtcaagt acggaacagc
    44881 caacgatgtc gtctaccagt gaagaacaaa aagaagaacc gaaacaacct gttgaaaaat
    44941 cagaagcaat tccaaaagaa aataaaccta ttgttccagc aaaagaagtc acagtatcgg
    45001 taaccccaag cggtgaaatt acgacgaata cttctcaagg aatgagtgta ccgattgtca
    45061 ctagtaatgt agaggaattg acccatattc caacaccaac caccccatta aaagtagcag
    45121 gtgatcaaac aattgttggt gtaaaagatg gtattccatt aatccaagat agtgaaggga
    45181 atttggtaaa ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaaatca
    45241 aaacagcaga tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgcac
    45301 tttctgttct aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga
    45361 gaaaaggaat agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc
    45421 gttgtcaatc gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac
    45481 aactacggcg gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt
    45541 caaaaaaagc agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct
    45601 tggcattttt tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc
    45661 aacaattaca aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg
    45721 aatcctttat gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag
    45781 aacaacaacg tcaagaaaca ttaaaaaaag agttttatgc acaaaacttt attcaagaac
    45841 caggaagtgt ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa
    45901 ttgaaaccat tccaactgct agttataaag taacttatga tattcgctcg atgaatccag
    45961 agagtaagaa agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt
    46021 caaacaaggg ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag
    46081 aaaaacaacg aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag
    46141 cgaaagatgt tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa
    46201 aagatatgtc atacatgatg aaagaacctg aaggactcaa tcaaacagct aaagttgatc
    46261 aagtagagat gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa
    46321 cggtcgtctt tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgattgtca
    46381 ttacgaaaaa aacaaatcag tattatgttg aaaaattaac acataattgg ttggatagga
    46441 aaggagaaga acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg
    46501 ctatttttta ataatggtgg ttgtttatct gggattgcga aattttatga aatcaaaagt
    46561 aggaaatgca ttaatatcta tcgcagcagg tgccgcagtt tatttcttct taaatgatcc
    46621 aacacgtgta tttgatgcgt ttggtaaaat cattgaaaaa ttatttatgg attaggagga
    46681 tagtgaagtg gattgggaac cttatgatta ttcgattgtt tatgcggaac cttatcgcgt
    46741 acaacagatt acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac
    46801 ttttttgttg gtcgtaatct ttgaatattt atttttacgt tcatttattg gttgggtcat
    46861 ttcttttttt ccaccagcag gagttgtttt ttatatagga ctcccctatt tatgtgtgtt
    46921 agcactggca aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt
    46981 ttgttatatg attcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc
    47041 aaacaatgca ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa
    47101 aaagggacaa tcaactggtt gttccttttt tattgggagg tgaaattcat tggtgcttgt
    47161 aaaagaacca gtaaaagcat tcaagaaaaa ttgtttgtta aatggagagg attgtatctg
    47221 ggcctactat catgtttttc cacgaacaat ttcaaaaaac acaccaaaaa agacacagga
    47281 aataaaagaa atgatggctc gtttactgtt gcttttagca gattatgaag atgtggagtt
    47341 ggtgatgtat ccacgaacaa tgaatttacg gacgaagttt gacacattag gaaagggatt
    47401 ttctcccgat agtcaagcaa ttggtgcgta ttatgcaaaa gaaacagtga cattgttgga
    47461 acgagaaggt caagtctatc gtccttcatt tattttagga atcaaagtca aagaaaaagc
    47521 aacggtggag agttttcatg atgtaataaa tctaatctgg ttagatttag caggaagttt
    47581 agcacgctta tttggattag aagtagatga tttggaaact ttgttttctg aaagtgatcc
    47641 ttatgtaaaa gaagtgacac aattacttta tagcttttct attcgtccct tagtaaaaaa
    47701 agagctgttg tatctcaatc gtctgcaata tattcgagga atggctcaca aagtagaaga
    47761 acaaaataat tgggaagtac tgaaagacaa tcaaggaacg tatgtacgta taccgaagga
    47821 acataaaggc cttcttcaat tagaagatga gttagggagt agttatttag cattgttgcc
    47881 tattggggaa acacctgaaa atatgagaaa ttgtgatcta tttcaatttg ctcaggatct
    47941 ttctttccca gcagaattgc gaatgaaaat tcactatcca cgcttgaaag gacagggagg
    48001 tattggttta aaactgaatt ggctatcgga acggttcaaa acagagcata aggaattggt
    48061 tcaaaatgat gaagaaccta gtgaacgctt attaactgtt cgaggattag taaaacattt
    48121 aaagcaacga gtagataatg ggcaacctgt cgtcgattgg atcgcatgtt ttattgtata
    48181 cggtcaaacg gttgaagaag ttcgtcaaaa tagtgcaacg gttattcaac aacttcatac
    48241 gattatccat atccaccgtg caaaaaatga tcaacgagaa ttgttctata aatgtttatt
    48301 agggcaacca ttgggacggc agttacattg gaagcaccga acaactgtag aagcagttgg
    48361 cgaatggttg ttttcagcag tatcagaatt aggtatggaa tccggctggt atattggtcg
    48421 gcaagataca ttaggggatg gtggttctcg aggagaaaaa acaccattag aagaattaat
    48481 ttatgcgtca aatcgttttg tctttttgaa tcttctagcc attgcggaag gaatcaaagg
    48541 tgcgttgttt gatgcgcctc atgtagcagt aactggaaaa accggtaaag ggaagacctt
    48601 tttagttggc ttgctctttt tgtatagttc gtttatgaat gtacaaagtt tatttgtgga
    48661 tcccaaagga gaaaagcgca aatggtttac gaaactcatt caagacccgt attatcagaa
    48721 acattaccct ttgtttgtag aacatttgca aaaaatgagt tatgtcacgt tagatgctac
    48781 aaatccccgt aattatggtg tattagatcc aattgtttgt ttatcccgag tagatgcgaa
    48841 acaagtagcc caggatatga ttgatgagtt aagtccgtta ggtaccaatc accttttaaa
    48901 aggggcggta ttaaagggaa tccaagaagt tttagttcaa aaagaagccg gacaacaagt
    48961 tggcttgatg catgtggtcg actatttaga acaaatggaa caaaaagagg ttcgagaata
    49021 tggtgagttt ttacgcttga cggtagaaga ttcgatttta cgtttaggat tttcttatgg
    49081 agaaaacccg ggattagact ttaatgccaa aacaacgatt ttagagatcc aagatttgaa
    49141 attaccaaat gacaatgtcc gaccagaact atatacagat gcggatcgaa agtctttgtg
    49201 tttgatgatt agtttaggtc gtttttgtga aatgtttgga aaacgagata gtagtaagaa
    49261 aacagcgatt tattttacag aagcatgggt atttaataat tcgaacgcag gacgtagtat
    49321 tattgcagca atggcacgtg tggggcgttc ccagatgaat caattagtgt tagatacaca
    49381 atttattggt gacttaggtt cagataaaga aaaagggaat tttggtgtcg tctttgcttt
    49441 tgatgaagac agtgagcgag aaaagatttt gaatcattta cgtttggaag taaatgaagc
    49501 gaacttagct gaaatgagcc acatgattaa aggacagtgt tggatgttag atccctatgg
    49561 acgtgtagga aaactcaatg tacacagttt gtttgaagaa atgaccgctg cattacaaac
    49621 aacggaaaaa acaagtcact ctcaagtaga agaacaggat acgttaatca tgcaatagaa
    49681 atgaggtgaa acaatggctt atataaagca taaggaaaag aaatggtgcg ttttgttttt
    49741 tttaacgatt ggattcggac tgctttttgt tggtggattg gtgtatgcgg cagaaaagca
    49801 ggtggatcaa tcgatctatg agattgggaa atttcatatt gatacctttt cttcttattt
    49861 atctgatagt gaattttcat taggcaaaat gttatttggg gcaaatggca cagattttct
    49921 aaaaatgatg acgaatatgt tctttagttt aagtaaattg atttggcaag gccttgattt
    49981 tgtcattgag aagctctatg aaggagctgc aatgaacgag ttaattcacc agtttttcac
    50041 tttttcggat actatttatt caaaaatata cgatcctatt ggtgcgggga ttgtcgtctt
    50101 gtatgtgtta tatctattta ctcttcgtat atttagaggg aaacattacg caaaaatggt
    50161 gtttattcga tttctcttcg ttgtcatttt ttcaatggtt tggtttggct atggtagtaa
    50221 cgtacctagt aaaggagaag cttttaccaa agggttaaat gatttgtccg tggaggtaga
    50281 aggctttatt ttttctgcta caaatagtct agatgaattg gcgacaaaag atgtcaatgg
    50341 tgttttagtg ccaacgacac aaaaggaagc cattcaaaaa gtgcgccaac tttattataa
    50401 agcggctgtt gttgatccgt atatattgtt aaattatggg acaacaaatt tagaagaatt
    50461 gtatcaagca gagattgaac cacaagaatt tctagccaag gaagcgtcaa gtgaagaatt
    50521 atctgatatt gagacaaaag tgaccactgc tgcaggagaa gatagaggga aaaagaaggc
    50581 ttatcgtgtc tatattcaac caactaaagc tgtttataaa gcagtcgttg gttctatggt
    50641 gccattgctt aatctaagta ttggattacc acttgtagtg attggcatga ttcgttttat
    50701 ctttcaaatg ttggtgttgt gtttattact tgtgattcct ttttcattac tcgcttcttt
    50761 tgttccagta tttgattata tgctcatgaa taccttaaag tcctttttgg gcttgatgtt
    50821 tcaaaaatct ttttacagtg tgattatttt ggtggcattt ttgatctata acatagtaga
    50881 cgatttaatt ccaatgaata ctgtttttgg ttttttagga aatatgtttg tcaaagcact
    50941 cctatcaatt ttactcattg taaaacggaa agatttatta aagaaactag ggttaggagc
    51001 tgcagatcaa actataaaca acacgaaaaa catagcaagt cattctttgt ctcaaatgaa
    51061 acaactgcct ggtcaactat caactgctgg acaaaatttg gcaatcaaag gtgcaggatt
    51121 agcagggaat gtgtatcgag caacgaatcc actcgcttct gcagcgatac atggatttca
    51181 aaagtttcaa caaaaacgta caccacaaga atcggtaaaa gggaggcaag tggttaatcc
    51241 ctttgaacta ggtgggcgtt caaatcaagc cttgccttat agtggtcgtc atgttcgtta
    51301 tcatggggta gagcctgaaa tgtcttctac caaatcgaaa acagagtgga attctggtct
    51361 ttcaaatggt ttgattccaa attcgtccat gaattcagta agaccaaaac aacaggtaca
    51421 atcattggca caattgagta agcaacaagg gaataatcaa agaaaaccat tgttaaataa
    51481 aggtatcgct tttgttggtc aatcgattcc gttgccttct ggaaaagtac ccgtgccaca
    51541 attaacagct gctggttctc tttcacaaca gattcgccaa gtgaaaaatc aggcgaaaaa
    51601 tgggagttat ccagcaatgc agcagttatt gaaccaatac tctgttccta attcgtcggt
    51661 agccactcta tcaggaaatc gttctgtgca acaaccgggg caacgttcgt ctgaaagtaa
    51721 accgtcgatt tccgcaccaa aatcgttaca acctgcattt tcttctttag ataagccaac
    51781 agagcaatcg gaaaaaatag aaaaggatcc taaaacactg ccgattttgg acgcaaaacc
    51841 aggaactgat tttactttac caaaaggacg aacagtccaa aaaccattat cgaagaaagg
    51901 atgaggaaga aatgaagaaa gggtgtctag gttgtcttct tttctttatt cttccctttt
    51961 ttctattgcc attctttttt tcaggagaac tttctgagca tggaggagaa gctacaggtc
    52021 tagctgtcgc ttttaatggg gaatataccg aaggattacc gattttttca gagattaaag
    52081 gacgtggtcc aatcacagat gaacatgcgc aagcagcagt tggtgcagct gtaaagtatc
    52141 agttattacc gagtgtgatt ctttctcaat taggctatga atcagcttat ggacagtcac
    52201 aatctggaag aaacgatacg aacttttttg ggattacctg gtttcctggt tgtccctacc
    52261 cacaaggttc tgcacgtgga attggtggtt ctgaaggtgg tttctatatg aagtttccga
    52321 acttaaaagc ttgttatgac tattatggct ttatggtggc gagccaaagc aactttaatc
    52381 aatgtgtggg caataaagat cctggaagtt gtttattgat tctaggtcgt ggaggctatg
    52441 cggcagcagg tattaccgaa gggagtccgt attatcgagg agcaatgagt atcataacgt
    52501 cctataactt aacggaatat gatacttttg cgatcaagca ttggaatagc ctaccaacaa
    52561 aaaaaacttc gcttattgca ggaaaaagta aaggcaatgc gaaggttttg gatcggatca
    52621 ttggacgatc cgtaaataat ggggaatgtt atggtggtac ggcatggtat gtatctcaat
    52681 taggtggccc acagttaatg ggttctggac acatgtttgc gatgttaata ggggaagatt
    52741 ataactggac aacctatggt tggcaagtaa tcatgaaccc aaaaccaagt gatttacaag
    52801 caggagatgt ggtgaattgg caagcaggtg gtgcattgtc tcctggaatc tatggtcaca
    52861 ccggaattat aacagatgtt tcatatggcg gacaacattt ctctagtgtg gaacagaatg
    52921 ccgagcaagg acggattatg gctcggtatc aacgcacgta caatcaaaca aaaattcgta
    52981 gtctggttcg taaggtgaaa taagaaaggc agtgactaga aacgtgaaaa aagggataat
    53041 tttagtattt agttggttat gtttgatagg tcttactggt tgtcattggt tttcttctga
    53101 aaaacaaaaa acacaattga gaacgacaga agaaacaatc gaagcagtaa aagaaacaag
    53161 tggaactctt caaagtactg aaagtaacag tcaaattggt tcattagaag acgagacagc
    53221 gcaacaaatg caaatagatg aaatgacaaa aaatttctta aaagattttg ctagaaaatg
    53281 gagtaatttc acagatgttt ataaacgcaa tcaagctgtg agggactata tgacagatcg
    53341 ttgtattaaa gacaatagta ttgatgtaga cccgcatgta caaatgaaaa caactgggaa
    53401 actttaccag gtgacacaag atatagagaa tccggatcaa tatttgttat ttggagaaga
    53461 aacaatgaat aaggttactc aatttgtatt acttcaattg aaagtagatc aagaagctca
    53521 aaagattgat gcgatcaaga tttattatgt acggagtgcg tattaaaata aaaaatgaac
    53581 actcatattt attatacacc gaagaaatag aaaaaaacag actgtttttt taagaaaaac
    53641 tatgtcattc tttacttgaa aaaagttgtt ataggacaga aaggataaaa caaaaacgga
    53701 aaaaatatgt cttatgaata actataagaa ctattctatt ttttaggaga tggatacgag
    53761 atgaaaaaac taattggcta tagtgtgatg tgtattggat ttgttggtgt gggattgttt
    53821 gtatcgactg aatctgcttc tgcggctgaa tggtataatc ctatctggta taatccgaac
    53881 attgaatgtc aaaaaggcta tccgtatgga ccgacaaatc tgaaaaatgg aaaatgtaga
    53941 gtaaattgga atggcgttgt agatagtgta attactggtt caatggactc tgctctacat
    54001 ggctttacgg gtcactagaa ggaggaagaa aatgtctgaa gagaaaaaag aaatcgttga
    54061 atcattagta gctttaaaag attctctttc taagattgaa tatgttgata ggcaagaaat
    54121 tcaaaaacta attgatgata ctattattga aatccaagat gcgcgttgtg aaggtataaa
    54181 aatttctgta gctttatcta aagtaataga aaaaatgaac cgttcattag cgtttaatgg
    54241 attgaagcta gatagacaaa ctagtctgat atgggatcat ttaaaagact tgtatgacaa
    54301 gtctaaaaga agtgaacgga ctgctgtatc aatcttaaaa ggcctttggg gaatgaattc
    54361 ataaacaaga tatttaaaaa gtaacttatt ttttatggag gagaaattat gaaaagaata
    54421 attaaaatat ttggtgtttt aggtgttttc ttattaggtt taagtacttt tgttagtact
    54481 ggaacaagtg tgtatgccac agaaaaagtg aatacagttg atttagaaat aaagaataaa
    54541 ttagtagatg aattaacttt ttattttgat acagttggtt ttttagacga acatggtgta
    54601 taccatataa aggattttgg tgctattcgt gaacgaatca gtcaagataa cgaacaagga
    54661 agagcagcaa gagtctttta tgaacatatt attgttccta gaatggagaa agttgattgg
    54721 aaacgatatg ctggatgtgt agccattaat tctcttcctg gaggaggagt ggcttgggct
    54781 ataggtacgc agggagattt tattgaagca ttaaaagctt ttaattttga aaaagctgcg
    54841 gatttattat taaaaattgc agaaaaacaa ttaggaaaag ctgcagctaa agagttagct
    54901 aaggtaaata ttgtagcagg aattgcagca gctgctgcta gctgtgcatt agagtaaaag
    54961 tttatgaaaa gtttagaaca agagcatatg gcttttaaac aagcaatgtt taaagaaaat
    55021 atctatttaa accataacta tattcgtgtt tctaaggcat gttcaccagt attgaatatg
    55081 cttggaggtg gaaatggatt atatcattta ctgtttgtcg atgtttgttg gttaattttt
    55141 ttaccagatg aactagttat agtcaatgaa aaaataacaa gtaaaaatga agtgtttaat
    55201 tattctttaa caagaattaa ttacaaagag attaccaagt tttcactaga aaaagttcct
    55261 ttttggggag aattttgttt aaaaattaaa tgtaactgga aaagaatgta tttctatatt
    55321 gatggtgatg atgctcttac attcggtaaa actacttttt ctagttttaa ttttcagttt
    55381 ctattaaaaa ataattttta tggtttattg aaataaggtt gcgaaggaag atgtagagtt
    55441 atttgcactg ttgtgttggt atatctctta atgctgtgtc tttttgggga gattaattag
    55501 tggagtgatt atatgagtat agatgattgt ttagaagaac aaataagatt atttttaaag
    55561 tttttaaaag ttaaagttca aaataactac ttcacatgtt atagttatgc acaccctgtt
    55621 ttaaatagtc ttccttttaa ttctttagtg caaggattga ttacaaatat tgttttatta
    55681 gtctgtacac caaatgaaat atttgtaaaa aagataggca
//
LOCUS       DACCQP010000013        54931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCQP010000013 DACCQP010000000
VERSION     DACCQP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.013"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            76..1074
                     /locus_tag="IVO87_000856"
     CDS             76..1074
                     /locus_tag="IVO87_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449073.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1314..1397)
                     /locus_tag="IVO87_000857"
     tRNA            complement(1314..1397)
                     /locus_tag="IVO87_000857"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1361..1363),aa:Leu,seq:gag)
     gene            1780..2997
                     /locus_tag="IVO87_000858"
     CDS             1780..2997
                     /locus_tag="IVO87_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7449074.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3181..4017
                     /locus_tag="IVO87_000859"
     CDS             3181..4017
                     /locus_tag="IVO87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449075.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4093..5241
                     /gene="nagA"
                     /locus_tag="IVO87_000860"
     CDS             4093..5241
                     /gene="nagA"
                     /locus_tag="IVO87_000860"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7449076.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5462..6520
                     /locus_tag="IVO87_000861"
     CDS             5462..6520
                     /locus_tag="IVO87_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7449077.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6539..7558
                     /locus_tag="IVO87_000862"
     CDS             6539..7558
                     /locus_tag="IVO87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7449078.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7660..8376)
                     /locus_tag="IVO87_000863"
     CDS             complement(7660..8376)
                     /locus_tag="IVO87_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7449079.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8345..9130)
                     /locus_tag="IVO87_000864"
     CDS             complement(8345..9130)
                     /locus_tag="IVO87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7449080.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9121..9870)
                     /locus_tag="IVO87_000865"
     CDS             complement(9121..9870)
                     /locus_tag="IVO87_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7449081.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10168..10692
                     /locus_tag="IVO87_000866"
     CDS             10168..10692
                     /locus_tag="IVO87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449082.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10708..12207
                     /locus_tag="IVO87_000867"
     CDS             10708..12207
                     /locus_tag="IVO87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7449083.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12295..12897)
                     /locus_tag="IVO87_000868"
     CDS             complement(12295..12897)
                     /locus_tag="IVO87_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7449084.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13001..13957
                     /locus_tag="IVO87_000869"
     CDS             13001..13957
                     /locus_tag="IVO87_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7449085.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14262..15218)
                     /locus_tag="IVO87_000870"
     CDS             complement(14262..15218)
                     /locus_tag="IVO87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7449086.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15392..15952
                     /locus_tag="IVO87_000871"
     CDS             15392..15952
                     /locus_tag="IVO87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449087.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15945..16769
                     /locus_tag="IVO87_000872"
     CDS             15945..16769
                     /locus_tag="IVO87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7449088.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16779..17396
                     /locus_tag="IVO87_000873"
     CDS             16779..17396
                     /locus_tag="IVO87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7449089.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17414..18097
                     /locus_tag="IVO87_000874"
     CDS             17414..18097
                     /locus_tag="IVO87_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7449090.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18779..20062
                     /locus_tag="IVO87_000875"
     CDS             18779..20062
                     /locus_tag="IVO87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7449091.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20078..20965
                     /locus_tag="IVO87_000876"
     CDS             20078..20965
                     /locus_tag="IVO87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449092.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20982..21818
                     /locus_tag="IVO87_000877"
     CDS             20982..21818
                     /locus_tag="IVO87_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7449093.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21821..22444
                     /locus_tag="IVO87_000878"
     CDS             21821..22444
                     /locus_tag="IVO87_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449094.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22441..23733
                     /locus_tag="IVO87_000879"
     CDS             22441..23733
                     /locus_tag="IVO87_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7449095.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24092..24189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24305..24886
                     /locus_tag="IVO87_000880"
     CDS             24305..24886
                     /locus_tag="IVO87_000880"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7449096.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24889..26196
                     /locus_tag="IVO87_000881"
     CDS             24889..26196
                     /locus_tag="IVO87_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7449097.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26612..27100
                     /gene="purE"
                     /locus_tag="IVO87_000882"
     CDS             26612..27100
                     /gene="purE"
                     /locus_tag="IVO87_000882"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7449098.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27084..28217
                     /gene="purK"
                     /locus_tag="IVO87_000883"
     CDS             27084..28217
                     /gene="purK"
                     /locus_tag="IVO87_000883"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7449099.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28230..29525
                     /gene="purB"
                     /locus_tag="IVO87_000884"
     CDS             28230..29525
                     /gene="purB"
                     /locus_tag="IVO87_000884"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7449100.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29705..30082)
                     /locus_tag="IVO87_000885"
     CDS             complement(29705..30082)
                     /locus_tag="IVO87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449101.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30338..31066
                     /locus_tag="IVO87_000886"
     CDS             30338..31066
                     /locus_tag="IVO87_000886"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7449102.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31066..31320
                     /gene="purS"
                     /locus_tag="IVO87_000887"
     CDS             31066..31320
                     /gene="purS"
                     /locus_tag="IVO87_000887"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7449103.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31322..31993
                     /gene="purQ"
                     /locus_tag="IVO87_000888"
     CDS             31322..31993
                     /gene="purQ"
                     /locus_tag="IVO87_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7449104.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31994..34216
                     /gene="purL"
                     /locus_tag="IVO87_000889"
     CDS             31994..34216
                     /gene="purL"
                     /locus_tag="IVO87_000889"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7449105.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34201..35640
                     /locus_tag="IVO87_000890"
     CDS             34201..35640
                     /locus_tag="IVO87_000890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7449106.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35672..36715
                     /locus_tag="IVO87_000891"
     CDS             35672..36715
                     /locus_tag="IVO87_000891"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7449107.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36712..37290
                     /gene="purN"
                     /locus_tag="IVO87_000892"
     CDS             36712..37290
                     /gene="purN"
                     /locus_tag="IVO87_000892"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7449108.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37287..38822
                     /gene="purH"
                     /locus_tag="IVO87_000893"
     CDS             37287..38822
                     /gene="purH"
                     /locus_tag="IVO87_000893"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7449109.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38838..40112
                     /gene="purD"
                     /locus_tag="IVO87_000894"
     CDS             38838..40112
                     /gene="purD"
                     /locus_tag="IVO87_000894"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7449110.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40234..41148
                     /locus_tag="IVO87_000895"
     CDS             40234..41148
                     /locus_tag="IVO87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7449111.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41281..42846)
                     /locus_tag="IVO87_000896"
     CDS             complement(41281..42846)
                     /locus_tag="IVO87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7449112.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42839..44290)
                     /locus_tag="IVO87_000897"
     CDS             complement(42839..44290)
                     /locus_tag="IVO87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7449113.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44304..45320)
                     /locus_tag="IVO87_000898"
     CDS             complement(44304..45320)
                     /locus_tag="IVO87_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7449114.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45540..46628
                     /locus_tag="IVO87_000899"
     CDS             45540..46628
                     /locus_tag="IVO87_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7449115.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46682..48208
                     /locus_tag="IVO87_000900"
     CDS             46682..48208
                     /locus_tag="IVO87_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7449116.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48210..49415
                     /locus_tag="IVO87_000901"
     CDS             48210..49415
                     /locus_tag="IVO87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449117.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49672..50649
                     /gene="guaC"
                     /locus_tag="IVO87_000902"
     CDS             49672..50649
                     /gene="guaC"
                     /locus_tag="IVO87_000902"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7449118.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50913..52337
                     /locus_tag="IVO87_000903"
     CDS             50913..52337
                     /locus_tag="IVO87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7449119.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52798..53907
                     /gene="pdhA"
                     /locus_tag="IVO87_000904"
     CDS             52798..53907
                     /gene="pdhA"
                     /locus_tag="IVO87_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7449120.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53910..54887
                     /locus_tag="IVO87_000905"
     CDS             53910..54887
                     /locus_tag="IVO87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7449121.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17563 a         9380 c        12000 g        15988 t
ORIGIN      
        1 tatgtttttg agaaaataaa tgttaaaaat ttattattgg tttgctctat atactgttta
       61 ataccattga cagtaatggg ggaaaccggt tggagagcaa ccaccttgaa ctatcagtgg
      121 ccagtagcat ttagtttact aactttttat ccgttttttc aactcttaag aggagaagaa
      181 ataaatagaa aaatatattg ggtaagtatt cctttattga tatttttaac taatcaagaa
      241 caagtcaacg cctgtttttt tgttctaaca agtatagtta gtttatattt gattgtcaat
      301 ggacgatata attataagtt atcagtattt tcaataataa gcttagcaga attaattttc
      361 tctttgacaa ctccagggaa tgcattacgt gctgcacatg aaattaataa atggtttcct
      421 gaatataaaa atttcaactt tcttaataag ttagatttag gtatttcatc atttggaaag
      481 ccgttttttt tagacatgaa tatcttattt ttattgctat ttttcttaat tttccttcta
      541 acatatagga aatgtcaaaa ctattatgtg cgtatattga ctgcattacc gtttttctta
      601 aacctgatta tttattttgg taatacaatg ggtcaaagct ttacttacgt caatggaaat
      661 aaaagggcaa tgatttggag tagtagtaac ctgaataatc tctttacaga gttaggaacg
      721 aaactgtcac ttttttatcc aggtacttgg atagctactt tagtagtctt ggcattatta
      781 ctatgtttaa ttgttggaat ttatttaagt tttgacaata agaaaacttc tattttttta
      841 gttattttga tgataatggg attttgttca aggttaatta tgggattttc tcccacagta
      901 tgggcttccg gaatgcggac gtactatata ctttatgttg ttatcgccat cttagtacta
      961 atggcagtta aagagctaat gaaaagcatg agtgtacaaa aaaatgaatt tatgcaattt
     1021 ggattaaccg tgcttggtat ttgtaccttt ataataacgg tgataaacag ataatataca
     1081 aaaaatccct cgctttagta ttaggtgagg gattttactc gtttttcgat taattgtttt
     1141 aacacttgca agtttctacg agtagcttgt ttccgacctt catatactca cctcaggctg
     1201 tttacatgat tgtacgttct ttttgtaaag gatacagtga tatgtatata atggcataaa
     1261 gaaacgttca aacaaaaaaa ctcgtgaagc aaaattaact tcacgagttg tagtgcagat
     1321 gatgggactc gaacccacac gagctaacgc tcacatgacc ctcaatcatg cttgtctgcc
     1381 aattccaaca catctgcgta ataataacac actaatgata gcgaggtttt acatagttgt
     1441 caagctcaaa aatgggagga cttattaaga tggagaaata tgacggagaa ttttcaggac
     1501 tgggaatgat cttaggtgta ttgattggac ttgcattcgg acgttttttg cttggtttta
     1561 tgctaggaat catttgtgga attgcaatgg attgggcagc gaatctatgg aatgactatc
     1621 atgacaatta agttctcaac gcttgtttgt gtttttttaa tacttacgtt tttcctaaat
     1681 aagtggcaaa attttctagg atggagtatg atattgaaat agtaaataac aaaaaatttg
     1741 gaaagttcta actttttgaa atgagggatt ggctaagtga tgagagaatt tgaaaaatcc
     1801 aacaaattag aaggtgtcag ttacgatgtt cgcggtcccg ttctagaaga agcggagcgg
     1861 atgcaagaag agggcattag tattttaaaa ttgaacacgg gaaatcccgc accttttgga
     1921 tttgaagcac caaacgaagt cattcgtgat ttgatcatga atgcccgtga ctcagaaggc
     1981 tactcagatt ccaaggggat cttttctgct agaaaagcaa ttgagcaata ttgccaactg
     2041 aaacattttc cgaatgtgac gatcaatgat atctacaccg gcaacggagt gagcgaattg
     2101 atcacgatgt gtatgcaggg gttgctggac aatggagatg aagtactggt tccaatgccg
     2161 gactatccgt tgtggacggc ttctattgca ttagctggag gcacacctgt tcattatatt
     2221 tgcgatgaag aggcagaatg gtatcctgat attgatgata tcaaatcaaa aataacgagt
     2281 cggacaaaag ctattgttat cattaatcct aataatccta ctggggcact ttatccaaaa
     2341 gaattattag aagaaatcgt tgaagtagct cgacaaaata acctgattat ttattccgac
     2401 gaaatctatg atcgcttagt gatggatggt ctagaacata ttccgattgc tacattagcg
     2461 cctgatttgt tcgttgttac gctgaacggt ctatccaaat ctcatcgtgt agcaggattt
     2521 cgagtaggct ggatggtcct aagtggagat aagtcacacg tcaaaggcta tatagaaggg
     2581 ctgaatatgt tgtcctccat gcgtttgtgt tccaatgtat tgtctcagca aattatccaa
     2641 accgcgctag gcggttatca aagtgtcgat gagctgctcc tgccaggcgg aaggatctat
     2701 gaacaaagag aatatatcta taatgcaatc aatgatattc ctggtctttc agccgtaaaa
     2761 ccaaaagctg ctttttatat attcccaaaa attgatacaa agcgattcaa tattttggat
     2821 gatgaaaaat tcgttttgga ctttttacat gaacatcata ttctacttgt acatggtggt
     2881 ggatttaatt ggaaccagcc agatcatttt cgaatcgttt atcttcctaa aatggatgat
     2941 ttgaaacaaa cagctaagaa aatgagagaa ttcctagcaa cctatcgcca aaaatgattt
     3001 taaaaaccaa atttttcagt aggagggcca cagacaaaca cactgaggct ctctttctag
     3061 tggagttttc ttaaataact aaagtatatt atttagttgc aagtcttttc ttgaagtttg
     3121 cgacttccta aagtaaaata cgaatagaat gcgaatacgc atattttggg gaggatactt
     3181 atggaaacgt taatcgagtc ctttggaacg atcattaaag agattcgaaa agaacaaaaa
     3241 atgacgcaaa aaatgctttc gcatgatatt tgttcacaaa gtgtattaag tcggatcgaa
     3301 aataatgagg aactgccgaa tgtattggtg atgatgcaga tttgtcaacg gctaggggta
     3361 acgattgatc atgtgatgaa tctctctcga aaaactactc ggacaaacga taaattattt
     3421 gaactgttgg atagttgttt ccaaaaacgt gattatcgga agttagaaca agtactaaaa
     3481 tcttctgata tttctgaaaa tctgtatcaa gcaactgact ttcaacgcta ttattattat
     3541 ctcggtgttt gcgaattcac gttgcgtcaa aatatctctc agtcccttta ttatttgaaa
     3601 gaagcattaa gctactctag ccaaaaagaa agaatccatg tttctgacac agaaatacaa
     3661 ctgattagtt gtatcggaaa aatatatggt gtggcaggaa aaacggctga agcacgctac
     3721 tatttgagtc gaagcatcca actgctgcat caatcagctg atgaacgatc aaaaagtgaa
     3781 ttttcgcaaa ttttttataa ttatggaaat ttcttattcc atcaaaatga aatcgacgaa
     3841 gcgttagaac aggtgaatca aggaatctat tgggctcaag agaagaatag ctactattac
     3901 ctcaatgatt tatttgtgct taaaagctta atatacaagc gtaaagaaga accagaaaga
     3961 gcagcattct atgaaggctt agcacaagct gtgaaacaaa tttcaaaaaa tttgtaaatg
     4021 gtgtagacca ttcttgactt cttctctaaa taagagtact ttagaggtag aaaaacaaag
     4081 ggagagaata gaatgagaac ttttatctat gctgacaaat tctttttagc gtcaggtgtc
     4141 aaacatgcag gctatcttga aattaccgat ggactttttg gtgaatacac caaagaacta
     4201 cctgagggag aagtcaacat cattgaccaa agcggcaaat ggattgctcc tggtttggta
     4261 gatacacata ttcacggcta tatgaatcat gatgtaatgg ataatgatgc agaaggcatc
     4321 aaagtgatgt ctgaaggtct gttatcttgt ggagtgactt catttttacc aacaacattg
     4381 acgtcaagca aagaacgttt aaaagatgta gctgagacaa tcggcaaaat gtatcaggaa
     4441 gcaccaggag ctaaaatcaa aggaatctat tttgaaggtc ctttcttcac agaagaacac
     4501 aaaggggcac aaaacccaag ttatttcggg gatcctgatt tggatacttt ccatgaatgg
     4561 caagaagctt caggtggttt aatcaaaaaa attgctttag caccggaacg tcaaggtgta
     4621 gaagagtttg tcaaacaagt gactgaagaa ggagtagttg tttctctagg acacagtaat
     4681 gctacactag aagaagcgca aaaagctgtt gaagcaggcg caagtgtatt tgtccatgct
     4741 ttcaatggta tgcgtggatt gaatcatcgc gaaccaggta tggtgggtgc gttattatct
     4801 ttagaacatg tcttttctga attgatttgt gatggccatc atgtacatcc aaatgctgcg
     4861 ggaatcctaa tggaaaaagc aggacatgat catgtcgcat taattacaga ctgtatgatg
     4921 gccggtggga tgcctgatgg aaattacaat ttaggcgaat ttccagtagt agtaaaagaa
     4981 ggaacagcac gtctagaatc tggcaactta gctggaagta tattgaaatt gaaagaagca
     5041 attaaaaatg ttgtagactg gggattagca acaccagaac aagcagtcat gatggctagt
     5101 ctgattccag caatcagctg taaaatcgat gatacatgtg gaaaaatcgc tcaaggacgc
     5161 gatgcagact ttatcgtatt gaaccctgat atgacattgg aagcaactta tctagatggc
     5221 gtggaacgtt tccacgcata atataattta agttaacctt aaaactaaac ttcttaagtt
     5281 tggttttaag gttttttttg tgttctagta aaaatcactt tacataagtt tcaaaagttt
     5341 ctttaatttc ctattgttca ataagtaaca atgtaaagat aaaattaaaa atgttaatac
     5401 gtattagcaa aataaaagct ttttttattg tgaataaatg aacataatta ggaggagaaa
     5461 gatgaactta tttgacattt ttcgatcgac tttgacagat atgtcgattg tcagtgcaat
     5521 cacttcaaca gtttttatta tattattagg gtttgtttgt agaaaaaagg ggattttttc
     5581 tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc cagcactggc
     5641 atttcgagct ttcatgcaag atatcaatac agaattactg atgcaaggaa tcaatgtctt
     5701 gatttgggga ttagttattt atattattct tattctagtt tcaaaacctt ttttcttaag
     5761 ctataaaggg gataagcaaa caacgttgcg tgtcctgacg atctttggtt ctacgacctt
     5821 tttcggaacg cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat
     5881 tttcaatatt ggctaccgaa tctttttgta ttcttatgga tacataaaaa tgagtggctt
     5941 gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt
     6001 tgccggcctt ttcatttggc tgttccaagg gtatctgcca caagtgtcag ttacaggtgc
     6061 agaaggagag ataagccaag tagcgttttt gcgaattgac cagactgcac cgtggctatt
     6121 tcagccgatg gattatttag ctggtttaag ttcaccattg gcgtggctag caatcggcgc
     6181 aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag
     6241 tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg attctagcag ttttgacggc
     6301 aacgaatatt ctaccagtca attttgaagc actggcaaca atcgttatca tgatggctac
     6361 accagctgca acagtggcag cagcctatgc tatcagtttt gatagggagg cacttttagc
     6421 ctccaatgca tccttactgt caactatttt tgctgtggta ctgacacctg tatggatcgt
     6481 tgtattacaa atgattcatg gcataggttt attttcataa gaaatggagg aagaaagtat
     6541 gagaatcgca tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa aattgaatat
     6601 ttaccattat gatctcagtc tttatgaaga actgttgaca catgacaata tcgtgtcagc
     6661 aaaggaccat gatgctgtat tgttgagggg aaattgtata gcagatgaga ccaatcttgc
     6721 aaaaatgcag gaatatggga ttcgttatgt ttttacacgg actgtgggag tcaatcatat
     6781 cgatctacaa gcggcagctg attttggtat gacagtagcc cgtgtaccat cgtattcgcc
     6841 aaatgcgata gcagagttat ctttgacttt tgccatgatg cttttgagaa atactgcgta
     6901 tacgacgatc cgaacatctt tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga
     6961 aatcagaaat tgcacagtag ggatcatagg aacaggcagg atagggttga cggaagctgt
     7021 tttgtttaag ggactaggag ccaaagtgat cggctacgat attcatcaga cagaagctgc
     7081 taaaaaagta ttgacgttcc actcgcttga agagttatta gccaaaagcg atattgtcag
     7141 tattcatgta cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag gatttttagc
     7201 tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt ggagagcttc aagataatga
     7261 agctatttta agagctttga aaagcaatca tttagctggt tttgcaaccg atgtttttgc
     7321 aaatgaaaca gaggtttttt tccgatcctt caagccatgg gagacgattc cagaccctgc
     7381 tatccagcaa ttagtagaaa tgtaccctag agttcttgta accccgcatg taggttcaaa
     7441 tacagatgag gcattatcaa atatgatttc gacaagtttc gagaactttc gtgaaataat
     7501 agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat taagataaga
     7561 aaacatggat attcgtcaat aaaatgttta aaaatagtcc aagtaaacag ttgttacttc
     7621 tgtatacttg gactattttg tatgggataa ttcgatgact caacagtaat ctgtgagctt
     7681 tttccgccca ttctttgtca attcataaca ggttagcggc cggccgattt ccaaatattt
     7741 gattctttcg ttcaatatat cttgttctac taaaaaattc aaatatttct tgacggatat
     7801 acgtgacagt gagctttttt gagccagttc ttttgttgaa aaaatagtat cttcttttag
     7861 aataaatgca cttatttttt ttaaagtcag ttttgacaga cctttaggca aatcgatgaa
     7921 tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca atttctgatc
     7981 ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga tcgccagctg
     8041 aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc ggatcacatt
     8101 ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc actgggatat catttccttg
     8161 ctgtctaagc caagataaaa aatggatacc actttcgcca ggcatgtata catcaagcag
     8221 cagcagatct gcgttttttt gtctgaaata tttttttcct tcttctacat ttctgaaact
     8281 ttcgattacg ttcatattag ggatgctttc cacaaaagta cgattcaatt ctgcaaccat
     8341 tggatcatct tcgattatca ggacatttgt catcattttg atcttccttt tttggtaatt
     8401 ctagatagac gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc
     8461 gtttgacgat tgaatggatg agattcaatc caaacccacg attgattccc ttcgtcgaaa
     8521 aacctagcgt aaatattttt tctgagattt cttctgatat cccgacaccg tcatcgcaga
     8581 cggtcacgat gaatacttca gcttctgtgt catagtttaa atgaatccgg acctgtccgt
     8641 ttctttcatg tttttgtagc acggcttctt ttgcattatc aagtaagttc cctaacactt
     8701 gcaaaagtga atggatggag tcatccatct ccattttagg gataaaagaa tcgctttcta
     8761 acagcatttc aacagattgt tctttggctt cgtttatttt gcctaataaa aaaccggcaa
     8821 ttgcaggtac tttgatcaga cttgagactt ggccaatctc ttttttataa ttttgattta
     8881 gctgctggat aaaatgggtg acttcttcat acttcttcaa atcaatcaaa ccagaaatga
     8941 catgaagttt attcatgaat tcatgggttt gagctctcat ggagtttata tattgtttcg
     9001 tccccccaag ctcctccatt aatttcatta cttctgatcg atttcgtaaa gttgcgactg
     9061 ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct agaaatagtt tctatcccat
     9121 ttaaatacat ggattgagtc cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa
     9181 cgtcatatag attatcactg atttttttcc tacttcgaaa tccaatagct gctctgtttg
     9241 gtagaaaatg ttttttgcag gtgtattgat caagaggata gttttatcag aagagatggc
     9301 taagattcct tcatctactt cattttcgat aatcgttttt tcacttaact gttctgctat
     9361 ttcttgtggt tcaagtccaa gcagtacttt tttcattttt tgggcaagaa aaacggcgcc
     9421 tatgatgcct acgctaagtc caataatcaa tgaaaaaaca atcatttttt gtccatcttt
     9481 gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa taatctcttg
     9541 atcataataa atagggacga aatagcggaa gccgaaccct aacattcctt ctttttcaga
     9601 gaaaaattct tgtcctccaa tcactttttt cacatcttca tgacttgaga aaggaagacc
     9661 gatgtaactt gggtctgcgt gagaatatcg aatgccttca gggtccatga caacgataaa
     9721 atctaaattc gatgcaatca tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc
     9781 accagtctgc agcttttttt ggatgagttt atcattggca acgattttag caatgctaga
     9841 gattttatct gacacactag atcggttcat cgaaagaata aaattcctga tcaatatggc
     9901 agaaacaatc aaagaaacga atgtggagaa acaaacgaca aggatgatcg tgttctggag
     9961 ctttaatggt tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa tttattgtga
    10021 aaattttaac atgaaaacaa aaatttaaca aattgtcaac gataaacaca accttcctat
    10081 aaaaaacacc actgagtcgg taaaagaagc tatacagaat tttcaaacat tggtatgata
    10141 gacgtagaaa tggaaggagg cgtgtatatg atagggggaa tcgcacttga gacgatttat
    10201 tggtatacat tgctgatttg tgcaggattg gcagtcctat tgattatttt tggagatgta
    10261 ttcgattttg atggcccgct tgatccgatg cttattatcc cttggttaac tttcacaagc
    10321 ttatttggtt ttctaggtga gcgtttaaca gatcaatcat ctttattttt atttattgct
    10381 agtggaggca tatcgatagt gttggtattt ttgcttaatt tctatatatt gatgccgatg
    10441 aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt
    10501 gtgatcacac cgatccctgt acgaggtatg ggagaaattc agttgaaaag tgttaccggt
    10561 tccattagtc gtccagcttg tttttacagc ccccaagaac agacagctga gcggggaacg
    10621 aaagtattga tcattgagat caaggaaaga atctgttatg tcatgcctta tgaaagcatg
    10681 ataaaagctt aaaggagcgt gtgttaaatg gaaataccag ttgtttttat cgcgatcgca
    10741 atcgcagctt tgattttact gatgttgctg atcgtctttg tagcgaagta ccagacagca
    10801 aaaccggatg aagcgttgat catcagtgga agttatttag gaagcaaaaa tgtgcacatt
    10861 gatgaaagca ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt
    10921 tttcaacgtt cgaatcggat cagtctatta tcgagtaaat tggacgtctc cacccctgaa
    10981 gtgtatacgg aacaaggcgt accggtgatg tgtgatggta cttcgatcat caaaatcggt
    11041 tcatctgtag aagagattgc tacagcagca gaacagtttt taggcaagac aagagaagaa
    11101 ctggaaaatg aagcaagaga agtattagaa ggacacttgc gctcgattct tggttctatg
    11161 actgtagaag aaatctatca aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct
    11221 tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa agaggtcaga
    11281 gacaaaaacg gttatcttga ctctttaggg aaaccaagaa tcgcccaagt caaaagagat
    11341 gcagatatag cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa
    11401 aaagaatcac aagcagctga attacagcga caaacagaga tcgcagaatc attaaaagaa
    11461 aaagaactga aattagcaac ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac
    11521 caagcctaca atttagaaag tgcccgagca caacaacaag taatcgaaca ggaaatgcag
    11581 attaaaatcg tagaacgtca aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt
    11641 gaaaagcaat atgattcgga agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa
    11701 caagaagcac aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga acgattccgt
    11761 gtggaagcac tggcagaagc ggaagccaat aagacacgtt tagctggaca agcagaagcc
    11821 gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc
    11881 ttcaaggaat atggcgaagc agctgtcttg agtatggtta tcgatatgct accgcaattg
    11941 atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt cgatacagga
    12001 agcagttcag gagaaaccag tggagcgaac cgagtgacaa attatgccac aaatttgctt
    12061 tcttctactc aagaaacact aaaagcgaca acaggacttg acttgaagga aatcgtagag
    12121 aatttgtcga cacgtgggac tactagccaa gtaaatatgt atgaagcgcc aaaagaggca
    12181 gaacaaaact cgatgaagga agaataaagt agtacacttc accgttcaat caaaaatgga
    12241 ggaagacttt ttaaagttct tctctccatt tttttatttt attgcaaaag aaaattagtc
    12301 ctcgctattt ttcttttgtt tttggatctt agcttctatc tcttgtaaat ttgttcttag
    12361 caatcgttct ttcttttgga gttcgaccaa atcttctgat agttcttcct tcttttctgt
    12421 ttcaggagga cgaagaaaga aattgatacc gattgcaatg aatgtcccta taaaagtatc
    12481 tagtacacgt tggatagcat acaaagcgga ttcgccttga gggatgctca acgcaatcag
    12541 caacaacgta gctattgcgg aaatgatacc ggaattattg ttaataccat ctgacacgac
    12601 gatcacaata ataactagaa taggaagcaa gatcaattct accaaaaaat cattgtgaaa
    12661 atagcttttg acaaaaaaat aaatgatagc taatgtacca cctaatgtat tgccgatgat
    12721 cctagacttt ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa aaacagcaga
    12781 taatgcagca atcatcggga ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa
    12841 aacagctaag gcacttttga tcgtacgcat acctagtcga aaacggccaa attgcataga
    12901 gattctcctt tttggtcttt ttactatcat acctaaactt ttgtctgata gctagttttt
    12961 cattattcaa gtataattaa aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac
    13021 agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca gcgggtagct tatcgctgtt
    13081 gtattggcca gctgcaggga tcattgcatt attaagtgta ggaaatacca agcgctctac
    13141 attgcgtacg ggcatgaatc gaatcgcagc ctttatatta gctacggcaa cagcactgtt
    13201 cagttttttt gctgttgggt atactatttt tggcttcagt ctttttttat tcctgttcat
    13261 cccgcttgcc tctcgcttca aattaaccga agggatagta gtaaattcgg ttttggtcac
    13321 tcattattta gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag cgggattaat
    13381 ggcgattgga ttagtatttg ctttgcttgc aaatgtttac atgccagatc gtacgaagca
    13441 gctgaaggaa aaccaaatac agatcgaaaa agaatttcga aatattttga gacaaatggc
    13501 agaatttctt ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac atttactgac
    13561 attcattcgc gaaagccaag aagatgccag agaacatcaa gaaaactact ggctcagaca
    13621 gccgctatac tacgaaactt acttctctat gagaagagca caggccaatg tgattaaaga
    13681 catgctggaa aatctagaga gaatccagca accagcttat tatggaaaac atatctatgg
    13741 tttattgatt tatactgcag aaacattttc agagtcaaat gatggcagac aaattttaac
    13801 acgtattgaa gaagtttatg tattgtatcg gcaaatgcct ctgccgacta gtcgccctga
    13861 attcgaggat cgagcagaat tgttccagtt tttgcagtct tttaaaagtt ttattgaaat
    13921 caaggcagaa ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc agctaatatt
    13981 caggtcaaaa agctgatttc acaacctctt ctttaaacgg tgttctcctg tcaactcctc
    14041 ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    14101 actcggagct gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa aagctttcct
    14161 ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
    14221 tattacttga tgcagaacag ctttttcaca accttttaaa attacatatt tttccataga
    14281 acttgcattt gatccaagat ttcgatgaca tcttttgtca agaagagaga gcgattgggc
    14341 aagtcacctt caatcatgtg taccatattc tttatttcgt aattcattgc ttcagatgta
    14401 ttcccgcttt cgatgaattc ctttgtacca tctgtaaata agatctccgc acgatcagca
    14461 cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg caacgatacc gacttttggc
    14521 atttttgcct gaaaagtcaa gctgacagca gcaagttcat tttctttgtt acgcaagata
    14581 gtgacggatt gttcgtccac gcctgtttca aaaggaacca ttgtagaggc aactacttca
    14641 ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat atccaacaga
    14701 gctcctccag ctaattctgg attgaagaac cggtttttag ggtctggttc tttgtaactg
    14761 ccgaaaggtg cttggatcat ttttaaagcc cccagttttc ctgtatccat tattgagcgt
    14821 aactgctgat aaagtggcat gttgaagatt gtcatcgctt ctgctaagat caaattcttt
    14881 tcttctgcta agcgcatagc gtcagccagt tcttttttat tcatcgtgat ggctttttca
    14941 catagaacgt gtttgcctgc ttccaatgct gccaagatat ggtcgatatg ctgtctattt
    15001 ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta acatttcttg atagcttccg
    15061 taagcttttg gaatattgta acggtgagcg aagttttccg ctttttctga agttctggaa
    15121 gcaactgctg ttagttcgct cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg
    15181 gtaccaagac caacgattcc ccattgatat tttttcatct gaaaacgctc cttcatagtt
    15241 ggattgagtt tcttgtctgt ctattattat aacaaaaatc acgaagaata aagaggagag
    15301 gacttgaaac attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt gatcaaaact
    15361 taagtagaat agttaaaagg aggggataaa cgtgcatcaa gtatatattg atcttattat
    15421 gagtgtatta ttagaacagt ttgaaactga aaaaaatttt ttagatgaat acttgctgat
    15481 cagtccagaa caatggcaaa aatggaaaga tggcgagaaa aatctgccat ctgaagcgat
    15541 gcagaaagta aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa agattttgcg
    15601 tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa
    15661 aacacgcatc gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc tgatcccttt
    15721 gaaaaataat cagcaggctg gtagatacct tgacttgaaa gtaagtattc aatatgatga
    15781 atggggctat gatgatattt tgaattttcg tctgccagca gatgttcaaa aacagatcga
    15841 aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa cttacgttca
    15901 tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat aaatttttag
    15961 gatttggagc agttgtgatt atgatcgcag caatgggcta tgttattttt agcgatgatg
    16021 attcgcatgt tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa acaactgcta
    16081 gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc
    16141 gtacgtccaa acaattgcca gatgttaaaa cagacgattg gaagctagtt ttggttggtc
    16201 cagatcacaa attagaaaaa gaaattgatg agtctactca attggcatca ttaagtaacg
    16261 gatacttgat cgacaaacgc attgctccag aatatgaagc atttgccaag gctgctgaag
    16321 cagctggatt tccattagtg atggtctcag cttaccggtc tgtatcatcc caacaacaag
    16381 ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga
    16441 caaagattac taaagaaacg attacggagc caggatacag cgaacaccac acaggtctgg
    16501 cagtcgatgt cgtcgatcaa aactggtaca acagttatcc ttctactgta ttggatgcaa
    16561 gttacggaga tcagcctgga gctaaatgga tcgcagataa tgctgcgcag tatggattta
    16621 tcgttcgtta tccaaaagga cacgaagata taactaagat cacatatgaa ccttggcact
    16681 tacgttatgt cggtaaagaa aacgctgagt atatcaccaa acaccaattg acgctagaag
    16741 aatatctgaa tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa
    16801 agcaaaagcg caagaatatc cgttggccag cagtggttgc cggtgtagca gtgatactct
    16861 tagcctttgt tttttccttg aagaatctgt cttctccttt tactgataat cagcaagaaa
    16921 atgaagcgca atcccatcag caatttattg aacggcttgt tccgcatgcc caagaactac
    16981 aagatggtta tggagtgctt cctagtatca ttttaggaca agctatatta gaatcaaatt
    17041 ggggaaaaag taccttggct agcaaataca ataatctttt cgggatcaag gcttatgggg
    17101 atcagaagaa agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg ataactatcc
    17161 aaggagactt caaagtgtat gattcatggg aacagtccat ggatgaccat acacaactgt
    17221 tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa agtgattacg gccaccaact
    17281 atcaggaagc cgcacaagca ttgcaagatg caggttatgc gacagacccc ggttatgcgc
    17341 aaaaaatcat ccaggtcatc gagacgtatc aattgaatca gtatgaccgg agatagcaaa
    17401 ggagaagtgg ctgatggcag aacaatttac gccagaaatc atgacggaat tgagaaaaga
    17461 tatcgtaaga gtaccggaaa tcattttaga agcaagcggg atcaagatat ttggaaaatt
    17521 gatccgttcg atcatcttta ctacggatat tgcgatcata cgtaatacaa atgcgaatgc
    17581 agtaattgct gtgtatcctt ttacacctca tccagcgatt actaaaagca ttattgaggc
    17641 agctgacatt ccagttttct ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc
    17701 ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga acggtcctac
    17761 gcctttagag acagtaaaag aagtttgtcg agtagttgat attccagtta tcagtacggt
    17821 aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct ggtatttcga ttatcaatat
    17881 cagtgcaggt aaagaaactg cagaagcagt acgccatttt cgtaaaaaat atcctgaact
    17941 tcctatcatt gcgacaggtg gtccaacgga agaaagtatt gctgagacca ttcaagcagg
    18001 agcaaatgcc atcacatata cgccaccaag tagcggagag ctttttagtc gtaaaatgga
    18061 aaaatatcgg gaaactgaga aaaagaatca tgaataacag gtgagaatca aaagacgagg
    18121 aagagcagat agtggctctt cctcgtcttt tgtactccag tactatttgt tcagaattca
    18181 aaaaaaagag aaattttttt gtttttttac ctgtaatgta cataagaaga gttaaaacct
    18241 tttgttatta ttagcattct aacaaaaaat gtcttgattt attgaagcgc tttctataga
    18301 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
    18361 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
    18421 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
    18481 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
    18541 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
    18601 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
    18661 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
    18721 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
    18781 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
    18841 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
    18901 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
    18961 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
    19021 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
    19081 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
    19141 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
    19201 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
    19261 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
    19321 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
    19381 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
    19441 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
    19501 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
    19561 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
    19621 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
    19681 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
    19741 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
    19801 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
    19861 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
    19921 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
    19981 tgaaaaagtc atttatgaag aagtaacagc agaagaagca gcagagagtc tttatgagtt
    20041 tgcgtcaaac attctaggat agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg
    20101 gttcttggat caagacaatg tggtcggtta cgtatttgct tctccattca tcctaggatt
    20161 tctattgata acagtgatac cgatgatctt gtcactttat tattcgttca ccgattatcg
    20221 tttgggaaat ccagtctcat ggatcggctt ggataattat ataaagatgc tgagtgatgc
    20281 ccgttttttg aactccatcc gagtcacttt tctctatgta tttgcatctg tacctgtgaa
    20341 actgatcttt ggtctattga ttgcttatct tttaacgagg aatatcaaag gaacgacttt
    20401 ctttcgttct ttgtattatg tgcctagttt gatcggtggc agtatttcta tcgctctagt
    20461 ttggcgggag ttgttttcta aaaacgggtt agtgaatatt ttcctggaac gattaggcgc
    20521 acctgcgctt tcctggttta gcgatcaaaa actggtcatg atccctcttg ttttgatgtc
    20581 tgcttggcaa ttcggttcgg caatgatcat ctttgctgca ggtatcaaag agatcccagg
    20641 ctcgtattat gaagcggcag agatcgatgg agcgaacaaa tggcagacgt tttacaagat
    20701 cacgttacct cttctttcgc cgatcatttt gtataacttg gttatgcaga caatcgcggc
    20761 gtttatgact tttacgcagg catttatcat tacccaaggt ggtccgaatg atgccacgaa
    20821 cttttatgct ctttatgtgt ataaccaagc tttttctttt aattctatgg ggtatgctag
    20881 tgctatttcg tggatcatgc tgttgattat gggagctgtc acctttctga ttttcaaatc
    20941 atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga aatgaataat acagtaacga
    21001 aatggtttca atacggtttt attttgctat taggtctagt catgatttat ccggtcttat
    21061 ggatgatcgc aggttctttt aaaacggatc aagagattat gagtggttcg ctgaatttga
    21121 ttccagagac ttttcaatgg gaaaattacg caaaaggatg ggcggggttt gctggaattt
    21181 cattttttac tttctttagg aattcgtttg tcatctctgc agtttcgacg atagggacag
    21241 ttctgagctc gacttgtatt gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat
    21301 tctggttcgt cgtcatgtta gtaacgatga tgattcctgc acaagtgatt ttgattccgc
    21361 agtttattat ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg attttaccac
    21421 atttttttgg acaagcattt ttcatttacc agatcatgca attcatggtc agcatcccca
    21481 aagagttaga tgaatcagcg attattgacg gatgcagcaa gtatagtgtc tttaccaaaa
    21541 ttatttttcc tttgctgaaa ccttcgatca tcacgacgat catcatccag ttttattgga
    21601 aatgggacga cttcatggga ccattgattt atttaaacaa gccgagatct tacacggtat
    21661 cgattgccat caaattattt gcagacgctg gatctagtac gacttacgcg agtatgtttg
    21721 cgatgtctac gttgtcgctg ctaccggttt ttctgatttt cttattcttc aataaatatc
    21781 ttgtccaggg gatcagtaca agcggtctga agggctgaat atgaaaaagc caatagaaga
    21841 gatgtttctt tacaagctca actactatgt ctgtcggatt tttttaacca gtcttatcag
    21901 taatgctctt ttcgctttag ttctaggatg tttgttcact ttaggattag tgctgtctgc
    21961 tcgtgttgtc tatctgattt attttttgat gatgtatcct tcttattatc tatctgtcca
    22021 ttacttggca gaaaaaaagg tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat
    22081 cattacgttg tttcagctgt ttgtcattat ttttgcagtt gattggtatt tattagtggg
    22141 aaagattgga gcttggtatc tgattccttt tctattgtgt ctgttgcttt ttgtatccca
    22201 tagttttttt agtacgctaa ttgatatctg caaagaagat aaagcagata gtcttgttta
    22261 tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt
    22321 atgccttttg tatggactgt tctggtgtat acagcatgat tttttactca ctgcattact
    22381 gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa
    22441 atgaagaaaa aatatgtaca agtcggactt ggtgggagag cgcgtttttt ttatgaggca
    22501 attgcctcta cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca
    22561 aggatggatt atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa agtaaagaca
    22621 tgtatgccat cacaatttga gcaactactg gatgacgtac ggccggctga agtcattatt
    22681 acgtcagtag atcgaacgca tcatgattat atcattcgtg caatgaaaaa aggatatgat
    22741 gttatttgcg aaaaacctct gactatcgat gaggaaaaag ctcaagaaat aataacggtc
    22801 caaaaacaga cgggaagaag cctgcgagtc acttttaatt atcgttatgc accgtatcac
    22861 acgaagataa aggaactgat agaaagtgat gtgattggtg atgtgttttc tatccacttc
    22921 gaatggctgt taaatacgca acacggggca gattatttca gaagatggca tcgaaataag
    22981 cacaatagcg gcgggcttct tgttcataaa tctacccatc attttgatct agtgaatttt
    23041 tggttgaaca gccgtcccca aaaggtattt gcattcggtg gattaaactt ttatgggatg
    23101 cataatgcgg agaaacgcgg agtagaaata ttttacagtc gatgctatgg aagcgaacta
    23161 gctaaagctg atccatttgc tatagacctg tcagccaatg aggaattgcg agaaatgtat
    23221 ttagaagcag aaaaagacag tggatatatt cgtgaccgca gtgtattttc cgatgatatc
    23281 tctattgaag atacactagg cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat
    23341 tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga
    23401 cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg caggtggaga taaaaaggat
    23461 gaaggagcag ccgttcttca gtccatccat gtctatccga tgttcggtga gccatatgag
    23521 gtaatcgtcg aacaagaagc gggcggacat ggcggaggag atccgcgttt attagatgat
    23581 ctatttggtg aagaaaaaga agaagatccg tggaatcggg cagcaactca cgtagatggt
    23641 attttgtcga ttttgacagg aatcgcagca aatcattcta ttgcttcagg aaaagcagta
    23701 gtgattgacg agcttgtcag cttaaaagaa taagaataga tttacggaaa ttacttcccg
    23761 tagtttataa aatcttgcct tattgccgat aggctgtctt ttgaacacta tttattcgga
    23821 aaaaggggct atgacatgct ttttgtcatg ccttcttttt atgtgggctg cttttttaga
    23881 taggagaaaa taaaaataac tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc
    23941 attagatttt acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac
    24001 tgttttcaat atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca
    24061 aaacagttgc aagaaaatga atttacacta ttattagttt tacataccta cttatatatc
    24121 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
    24181 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
    24241 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
    24301 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
    24361 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
    24421 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
    24481 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
    24541 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
    24601 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
    24661 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
    24721 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
    24781 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
    24841 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
    24901 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
    24961 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
    25021 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
    25081 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
    25141 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
    25201 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
    25261 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
    25321 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
    25381 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
    25441 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
    25501 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
    25561 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
    25621 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
    25681 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
    25741 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
    25801 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
    25861 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
    25921 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
    25981 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
    26041 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
    26101 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
    26161 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
    26221 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
    26281 gtttgtttga gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg
    26341 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg
    26401 ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag
    26461 gaatgtgacc taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa
    26521 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
    26581 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
    26641 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
    26701 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
    26761 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
    26821 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
    26881 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
    26941 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
    27001 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
    27061 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
    27121 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
    27181 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
    27241 attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
    27301 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
    27361 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
    27421 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
    27481 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
    27541 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
    27601 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
    27661 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
    27721 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
    27781 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
    27841 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
    27901 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
    27961 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
    28021 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
    28081 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
    28141 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
    28201 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
    28261 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
    28321 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
    28381 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
    28441 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
    28501 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
    28561 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
    28621 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
    28681 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
    28741 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
    28801 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatctcta
    28861 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
    28921 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
    28981 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
    29041 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
    29101 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
    29161 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
    29221 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
    29281 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
    29341 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
    29401 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
    29461 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
    29521 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa
    29581 gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata
    29641 ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga
    29701 ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca
    29761 gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat
    29821 aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt
    29881 ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga
    29941 tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca
    30001 gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac
    30061 cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta
    30121 gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt
    30181 ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt
    30241 gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg
    30301 tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta
    30361 tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata
    30421 tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga
    30481 attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa
    30541 tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc
    30601 tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc
    30661 cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga
    30721 tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat
    30781 cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa
    30841 ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt
    30901 gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca
    30961 tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt
    31021 atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa
    31081 gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca
    31141 gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata
    31201 caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg
    31261 cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa
    31321 catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc
    31381 agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga
    31441 aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg
    31501 tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa
    31561 accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg
    31621 tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa
    31681 ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc
    31741 tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa
    31801 tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat
    31861 tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga
    31921 cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa
    31981 ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag
    32041 aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt
    32101 aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg
    32161 ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt
    32221 gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt
    32281 caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg
    32341 agttggcgga atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga
    32401 ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt
    32461 tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc
    32521 atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga
    32581 acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg
    32641 tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca
    32701 agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct
    32761 gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga
    32821 tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg
    32881 acttatattg acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat
    32941 gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa
    33001 aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga
    33061 cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc
    33121 attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga
    33181 atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga
    33241 tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca
    33301 agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg
    33361 gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc
    33421 aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg
    33481 taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt
    33541 ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc
    33601 tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac
    33661 accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt
    33721 caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag
    33781 tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc
    33841 cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag
    33901 tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca
    33961 tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca
    34021 atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg
    34081 agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac
    34141 tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt
    34201 atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat
    34261 ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa
    34321 ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg
    34381 ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc
    34441 ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta
    34501 ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt
    34561 tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag
    34621 atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt
    34681 gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt
    34741 gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca
    34801 tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat
    34861 gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg
    34921 aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag accagattca
    34981 aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa
    35041 gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca
    35101 agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac
    35161 attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa
    35221 ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt
    35281 gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa
    35341 gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt
    35401 caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc
    35461 ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa
    35521 aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg
    35581 ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa
    35641 aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt
    35701 agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga
    35761 acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt
    35821 tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt
    35881 ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa
    35941 tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa
    36001 aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg
    36061 aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga
    36121 ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga
    36181 aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg
    36241 gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact
    36301 gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc
    36361 ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg
    36421 agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact
    36481 tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga
    36541 gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc
    36601 gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg
    36661 gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc
    36721 gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa
    36781 aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg
    36841 aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca
    36901 aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc
    36961 gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga
    37021 atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat
    37081 gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta
    37141 gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat
    37201 tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa
    37261 attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa
    37321 ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag
    37381 gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga
    37441 cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg
    37501 gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc
    37561 aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag
    37621 atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta
    37681 gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag
    37741 tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg
    37801 caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt
    37861 gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc
    37921 gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat
    37981 attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag
    38041 atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga
    38101 aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag
    38161 cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag
    38221 atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt
    38281 atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag
    38341 actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc
    38401 ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac
    38461 gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg
    38521 tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc
    38581 agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag
    38641 gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca
    38701 aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg
    38761 atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt
    38821 agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa
    38881 taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc
    38941 cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt
    39001 taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt
    39061 tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa
    39121 aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc
    39181 aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca
    39241 aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag
    39301 tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc
    39361 gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct
    39421 cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata
    39481 agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag
    39541 taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg
    39601 ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg
    39661 ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc
    39721 aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga
    39781 atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag
    39841 ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag
    39901 agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag
    39961 gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt
    40021 acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa
    40081 aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac
    40141 aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac
    40201 ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct
    40261 gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca
    40321 gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt
    40381 tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa
    40441 cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt
    40501 tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat
    40561 gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa
    40621 gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa
    40681 ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg
    40741 caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat
    40801 gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg
    40861 atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa
    40921 aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca
    40981 actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt
    41041 acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg
    41101 gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa
    41161 gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc
    41221 gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta
    41281 ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg
    41341 ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat
    41401 atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc
    41461 tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga
    41521 gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt
    41581 ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg
    41641 tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc
    41701 gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat
    41761 tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa
    41821 tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc
    41881 gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg
    41941 aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc
    42001 ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc
    42061 gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa
    42121 cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat caactattaa
    42181 aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa
    42241 aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc
    42301 tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt
    42361 caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt
    42421 caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt
    42481 tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc
    42541 atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa
    42601 tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat
    42661 gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag
    42721 tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac
    42781 agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt
    42841 atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg
    42901 ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa
    42961 ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt
    43021 tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta
    43081 taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt
    43141 gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt
    43201 ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc
    43261 cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag
    43321 gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca
    43381 tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt
    43441 tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat
    43501 ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca
    43561 tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt
    43621 tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc
    43681 tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt
    43741 ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt
    43801 cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta
    43861 cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca
    43921 gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa
    43981 tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta
    44041 ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac
    44101 tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga
    44161 tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga
    44221 taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat
    44281 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
    44341 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
    44401 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
    44461 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
    44521 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
    44581 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
    44641 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
    44701 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
    44761 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
    44821 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
    44881 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
    44941 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
    45001 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
    45061 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
    45121 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
    45181 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
    45241 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
    45301 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
    45361 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
    45421 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
    45481 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
    45541 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
    45601 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
    45661 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
    45721 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
    45781 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
    45841 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
    45901 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
    45961 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
    46021 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
    46081 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
    46141 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
    46201 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
    46261 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
    46321 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
    46381 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
    46441 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
    46501 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
    46561 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
    46621 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
    46681 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
    46741 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
    46801 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
    46861 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
    46921 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
    46981 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
    47041 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
    47101 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
    47161 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
    47221 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
    47281 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
    47341 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
    47401 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
    47461 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
    47521 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
    47581 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
    47641 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
    47701 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
    47761 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
    47821 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
    47881 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
    47941 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
    48001 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
    48061 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
    48121 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
    48181 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
    48241 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
    48301 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
    48361 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
    48421 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
    48481 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
    48541 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
    48601 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
    48661 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
    48721 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
    48781 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
    48841 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
    48901 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
    48961 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
    49021 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
    49081 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
    49141 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
    49201 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
    49261 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
    49321 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
    49381 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
    49441 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
    49501 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
    49561 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta
    49621 aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta
    49681 tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa
    49741 tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac
    49801 atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat
    49861 atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt
    49921 ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag
    49981 ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct
    50041 aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt
    50101 gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca
    50161 acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg
    50221 actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc
    50281 atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg
    50341 acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa
    50401 gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc
    50461 gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat
    50521 acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat
    50581 ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac
    50641 acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta
    50701 ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg
    50761 cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca
    50821 cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa
    50881 aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag
    50941 gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca
    51001 atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt
    51061 ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg
    51121 gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt
    51181 ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag
    51241 aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg
    51301 agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa
    51361 aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa
    51421 gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag
    51481 caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac
    51541 cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac
    51601 cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat
    51661 tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa
    51721 aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg
    51781 atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg
    51841 atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa
    51901 tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga
    51961 tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa
    52021 ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg
    52081 cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct
    52141 ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg
    52201 gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt
    52261 acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa
    52321 agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt
    52381 aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa
    52441 aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact
    52501 gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa
    52561 aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta
    52621 atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc
    52681 tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag ttttattcac
    52741 tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg
    52801 gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc
    52861 ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat
    52921 ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac
    52981 caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga
    53041 caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca
    53101 ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca agctttttta
    53161 tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca
    53221 caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa
    53281 cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat
    53341 ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa
    53401 aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca
    53461 caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca
    53521 gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta
    53581 atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc
    53641 taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt
    53701 aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca
    53761 actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt
    53821 tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca
    53881 atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca
    53941 cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg
    54001 ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag
    54061 accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg
    54121 cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa
    54181 tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc
    54241 tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg
    54301 ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc
    54361 cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc
    54421 tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag
    54481 ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg
    54541 gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg
    54601 ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg
    54661 tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg
    54721 cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac
    54781 gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa
    54841 atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac
    54901 gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DACCQP010000014        51060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCQP010000014 DACCQP010000000
VERSION     DACCQP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.014"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            178..1023
                     /locus_tag="IVO87_000906"
     CDS             178..1023
                     /locus_tag="IVO87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7449122.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1304..2524
                     /locus_tag="IVO87_000907"
     CDS             1304..2524
                     /locus_tag="IVO87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7449123.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2521..5241
                     /locus_tag="IVO87_000908"
     CDS             2521..5241
                     /locus_tag="IVO87_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7449124.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5225..6169
                     /locus_tag="IVO87_000909"
     CDS             5225..6169
                     /locus_tag="IVO87_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7449125.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6238..7248)
                     /locus_tag="IVO87_000910"
     CDS             complement(6238..7248)
                     /locus_tag="IVO87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7449126.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7428..7772)
                     /locus_tag="IVO87_000911"
     CDS             complement(7428..7772)
                     /locus_tag="IVO87_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7449127.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7765..8199)
                     /locus_tag="IVO87_000912"
     CDS             complement(7765..8199)
                     /locus_tag="IVO87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7449128.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8337..9074
                     /locus_tag="IVO87_000913"
     CDS             8337..9074
                     /locus_tag="IVO87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449129.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9067..10278
                     /locus_tag="IVO87_000914"
     CDS             9067..10278
                     /locus_tag="IVO87_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449130.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10485..11264
                     /locus_tag="IVO87_000915"
     CDS             10485..11264
                     /locus_tag="IVO87_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7449131.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11334..11996
                     /gene="trmB"
                     /locus_tag="IVO87_000916"
     CDS             11334..11996
                     /gene="trmB"
                     /locus_tag="IVO87_000916"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7449132.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12094..13848)
                     /gene="ade"
                     /locus_tag="IVO87_000917"
     CDS             complement(12094..13848)
                     /gene="ade"
                     /locus_tag="IVO87_000917"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7449133.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13980..14909
                     /gene="cpaF"
                     /locus_tag="IVO87_000918"
     CDS             13980..14909
                     /gene="cpaF"
                     /locus_tag="IVO87_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7449134.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14866..15915
                     /locus_tag="IVO87_000919"
     CDS             14866..15915
                     /locus_tag="IVO87_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7449135.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15912..16190
                     /locus_tag="IVO87_000920"
     CDS             15912..16190
                     /locus_tag="IVO87_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7449136.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16165..16635
                     /locus_tag="IVO87_000921"
     CDS             16165..16635
                     /locus_tag="IVO87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7449137.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16652..16954
                     /locus_tag="IVO87_000922"
     CDS             16652..16954
                     /locus_tag="IVO87_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449138.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16971..17342
                     /locus_tag="IVO87_000923"
     CDS             16971..17342
                     /locus_tag="IVO87_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7449139.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17314..17673
                     /locus_tag="IVO87_000924"
     CDS             17314..17673
                     /locus_tag="IVO87_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449140.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17845..18852
                     /locus_tag="IVO87_000925"
     CDS             17845..18852
                     /locus_tag="IVO87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7449141.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18877..20061
                     /locus_tag="IVO87_000926"
     CDS             18877..20061
                     /locus_tag="IVO87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7449142.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20498..22081)
                     /locus_tag="IVO87_000927"
     CDS             complement(20498..22081)
                     /locus_tag="IVO87_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7449143.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22659..22802)
                     /locus_tag="IVO87_000928"
     CDS             complement(22659..22802)
                     /locus_tag="IVO87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7449144.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23025..23504)
                     /locus_tag="IVO87_000929"
     CDS             complement(23025..23504)
                     /locus_tag="IVO87_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7449145.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23577..23861)
                     /locus_tag="IVO87_000930"
     CDS             complement(23577..23861)
                     /locus_tag="IVO87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449146.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23939..24133)
                     /gene="ssrS"
                     /locus_tag="IVO87_000931"
     ncRNA           complement(23939..24133)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVO87_000931"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24205..25491)
                     /locus_tag="IVO87_000932"
     CDS             complement(24205..25491)
                     /locus_tag="IVO87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7449147.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25629..26300
                     /locus_tag="IVO87_000933"
     CDS             25629..26300
                     /locus_tag="IVO87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7449148.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26266..26754
                     /locus_tag="IVO87_000934"
     CDS             26266..26754
                     /locus_tag="IVO87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7449149.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26767..27714
                     /gene="prmA"
                     /locus_tag="IVO87_000935"
     CDS             26767..27714
                     /gene="prmA"
                     /locus_tag="IVO87_000935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7449150.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27715..28479
                     /locus_tag="IVO87_000936"
     CDS             27715..28479
                     /locus_tag="IVO87_000936"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7449151.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28712..30925
                     /locus_tag="IVO87_000937"
     CDS             28712..30925
                     /locus_tag="IVO87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7449152.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30936..31382
                     /locus_tag="IVO87_000938"
     CDS             30936..31382
                     /locus_tag="IVO87_000938"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7449153.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31553..32812
                     /locus_tag="IVO87_000939"
     CDS             31553..32812
                     /locus_tag="IVO87_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7449154.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32901..33848)
                     /locus_tag="IVO87_000940"
     CDS             complement(32901..33848)
                     /locus_tag="IVO87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7449155.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34242..35534
                     /locus_tag="IVO87_000941"
     CDS             34242..35534
                     /locus_tag="IVO87_000941"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7449156.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35549..37318
                     /gene="aspS"
                     /locus_tag="IVO87_000942"
     CDS             35549..37318
                     /gene="aspS"
                     /locus_tag="IVO87_000942"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7449157.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37427..38302
                     /locus_tag="IVO87_000943"
     CDS             37427..38302
                     /locus_tag="IVO87_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7449158.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38568..40451
                     /locus_tag="IVO87_000944"
     CDS             38568..40451
                     /locus_tag="IVO87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7449159.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40526..40810)
                     /locus_tag="IVO87_000945"
     CDS             complement(40526..40810)
                     /locus_tag="IVO87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7449160.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40800..41534)
                     /locus_tag="IVO87_000946"
     CDS             complement(40800..41534)
                     /locus_tag="IVO87_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7449161.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41663..42286
                     /locus_tag="IVO87_000947"
     CDS             41663..42286
                     /locus_tag="IVO87_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7449162.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42478..43599
                     /locus_tag="IVO87_000948"
     CDS             42478..43599
                     /locus_tag="IVO87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7449163.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43606..46734
                     /locus_tag="IVO87_000949"
     CDS             43606..46734
                     /locus_tag="IVO87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7449164.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46753..49947
                     /locus_tag="IVO87_000950"
     CDS             46753..49947
                     /locus_tag="IVO87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7449165.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50093..50800)
                     /locus_tag="IVO87_000951"
     CDS             complement(50093..50800)
                     /locus_tag="IVO87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7449166.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16708 a         8933 c        10615 g        14804 t
ORIGIN      
        1 tttggattca attcatattt atttataaat caaaaataca gagaatagta tataaatgtc
       61 ctttcctatt cttaaaatga tgtttgtact tacttaaatt aggtataatt agtatatgtt
      121 ttgtgagaaa tttagactta caataagcag tgaatttata atcaacgtat aggagacatg
      181 gatgaaatga aactaattgg tatagattta gacggtactc ttttgaattc tgaccagagc
      241 attagtcaaa aaaatgcaag aacaatgaaa catttaccgg aagactgttt tccattcatt
      301 tgttctggaa gagaagtaga agacattaat aatattctca aaaaatctgg tttaaggata
      361 ccagcagtag ggttaaatgg ttctgttgga tttgatggag aaaaaaagat atttgagtct
      421 tccttcgata aaaatgatgt aaaaaaaatt gattctgtcc tatcgaaatt tccaaccaaa
      481 atatatacaa atctcggaag ttatgaatct cagaattata aaaataaaat gaaaaaaatt
      541 tttcacgagg tagggagtga attttctatc aaagagctaa actatgagct tgagtatgaa
      601 aaatctattt gttcaatccc ttataataat attgaggaaa ttatagaaaa ggatgctatc
      661 agcatttaca aattttttat ttttattcct aatcgtcaat tgaaagctaa tatatttaac
      721 catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg cacctgtaaa tattgaaatc
      781 gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt tggaatcaat ctacaattta
      841 aagaaacctt tgagaatcgc gatcggagat agtttaaatg acttatctat gtttgaaagt
      901 gcagatatta gttttgcaat gggaaatagt catcaagaaa tcatgaaaaa agctgattat
      961 gtcactgttt caaatgataa agacggagta tctcaagcat ttgaaagaat tcttaccata
     1021 taaactaatt aaggtagtat agaacataga attctaaata cggaaaaatg gagcataaat
     1081 tccagtacaa atagtacttc ttcagtgtca aacgaagcga atataacata gatatttagg
     1141 attgtgacaa aaatcatgtc acaatctttt tttcgaataa acttattttt tgaagtcggc
     1201 catttatcgt aatttctttg cataatacga acaaattttc ggttattgat gaaaaaaaac
     1261 attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga aatttatcca
     1321 tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg ataaaactgt
     1381 acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat
     1441 aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc
     1501 attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga accatatacc
     1561 tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt
     1621 tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa
     1681 aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga
     1741 caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt tgtaccacgg
     1801 ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa agatgcaaga
     1861 aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct taaatgaaaa
     1921 gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac aagcttctac
     1981 atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga ttaaagtagc
     2041 agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga caaaagaggt
     2101 cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt taattgagat
     2161 aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac aatcaggaga
     2221 gctattagac tatttgttag aggaattctc agataggata aatgatttct ttatttggcg
     2281 tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt ctgctaaatt
     2341 gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga
     2401 agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat atcaagaaga
     2461 aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg aggatcagga
     2521 atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgac
     2581 ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc aacgatttat
     2641 caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa aaaggattat
     2701 acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc tgtatatggt
     2761 gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg
     2821 gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac gaaagagtta
     2881 ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca tttgagagaa
     2941 aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca agtattccaa
     3001 aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg ccaaaaatta
     3061 ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa
     3121 gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag
     3181 cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc cagacagcaa
     3241 gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag cactacaggt
     3301 cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta tcagcaaaca
     3361 aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat ggatcagcag
     3421 tcagccagat attattttta tttagagcag gagcaggaac taaaagatct ccagcaactt
     3481 caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga gactagagaa
     3541 acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg
     3601 gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag aaggattgct
     3661 gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca agagatgatt
     3721 gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa tcagtcagag
     3781 atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc aataatcatt
     3841 gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg tgcaggcatg
     3901 attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga tttctctgag
     3961 ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc tcaattagct
     4021 gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca gcaagaagta
     4081 tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact catgaaagaa
     4141 tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg gaaacataaa
     4201 agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga gtttgttaaa
     4261 gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct ttcccgtttt
     4321 atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag
     4381 caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata cagcgaacaa
     4441 ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa
     4501 caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac tccactattt
     4561 cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa agaaaagcaa
     4621 tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa
     4681 gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc tagccggcaa
     4741 aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc cgggacattc
     4801 ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt aagagcagca
     4861 ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact gaaaaatgat
     4921 acactgactt taacagacgg atatacagaa tttcccatct atgagttatc tacaggtacg
     4981 aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc taaccgttcg
     5041 atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg aaagtatcaa
     5101 ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat
     5161 aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt agaaggagca
     5221 aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat ttgtcctgat
     5281 caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca
     5341 agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg aaatcaataa
     5401 atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc
     5461 tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag agcctagttt
     5521 atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattgtt
     5581 gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta ataaatatca
     5641 aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg ccagtggatt
     5701 agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg aatatcctga
     5761 gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa aggtgattga
     5821 actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg gtcatctggt
     5881 attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga
     5941 agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag aatatggctc
     6001 acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca atatcgatgc
     6061 ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg
     6121 gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat
     6181 ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg ttttctttta
     6241 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
     6301 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
     6361 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
     6421 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
     6481 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
     6541 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
     6601 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
     6661 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
     6721 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
     6781 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
     6841 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
     6901 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
     6961 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
     7021 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
     7081 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
     7141 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
     7201 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     7261 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     7321 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     7381 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     7441 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     7501 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     7561 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     7621 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     7681 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     7741 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     7801 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     7861 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     7921 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     7981 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     8041 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     8101 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     8161 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     8221 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     8281 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     8341 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     8401 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     8461 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     8521 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     8581 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     8641 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     8701 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     8761 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     8821 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     8881 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     8941 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     9001 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     9061 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     9121 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     9181 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     9241 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     9301 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     9361 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     9421 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     9481 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     9541 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     9601 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     9661 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     9721 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     9781 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     9841 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     9901 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     9961 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
    10021 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
    10081 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
    10141 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
    10201 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
    10261 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
    10321 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
    10381 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
    10441 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
    10501 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
    10561 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
    10621 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
    10681 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
    10741 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
    10801 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
    10861 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
    10921 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
    10981 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
    11041 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
    11101 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
    11161 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
    11221 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
    11281 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
    11341 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
    11401 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
    11461 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
    11521 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
    11581 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
    11641 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
    11701 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
    11761 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
    11821 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
    11881 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
    11941 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
    12001 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
    12061 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
    12121 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
    12181 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
    12241 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
    12301 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
    12361 tttgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
    12421 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
    12481 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
    12541 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
    12601 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
    12661 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
    12721 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
    12781 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
    12841 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
    12901 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
    12961 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
    13021 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
    13081 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
    13141 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
    13201 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
    13261 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
    13321 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
    13381 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
    13441 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
    13501 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
    13561 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
    13621 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
    13681 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
    13741 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
    13801 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
    13861 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
    13921 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
    13981 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
    14041 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
    14101 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
    14161 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
    14221 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
    14281 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
    14341 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
    14401 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
    14461 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
    14521 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
    14581 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
    14641 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
    14701 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
    14761 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
    14821 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
    14881 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
    14941 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
    15001 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
    15061 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
    15121 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
    15181 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
    15241 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
    15301 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
    15361 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
    15421 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
    15481 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
    15541 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
    15601 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
    15661 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
    15721 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
    15781 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
    15841 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
    15901 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
    15961 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
    16021 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
    16081 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
    16141 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
    16201 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    16261 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    16321 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    16381 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    16441 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    16501 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    16561 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    16621 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    16681 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    16741 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    16801 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    16861 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    16921 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    16981 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    17041 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    17101 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    17161 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    17221 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    17281 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    17341 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    17401 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    17461 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    17521 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    17581 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    17641 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    17701 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    17761 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    17821 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    17881 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    17941 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    18001 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    18061 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    18121 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    18181 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    18241 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    18301 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    18361 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    18421 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    18481 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    18541 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    18601 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    18661 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    18721 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    18781 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    18841 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    18901 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    18961 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    19021 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    19081 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    19141 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    19201 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    19261 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    19321 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    19381 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    19441 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    19501 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    19561 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    19621 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    19681 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    19741 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    19801 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    19861 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    19921 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    19981 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    20041 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    20101 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    20161 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    20221 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    20281 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    20341 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    20401 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    20461 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    20521 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    20581 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    20641 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    20701 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    20761 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    20821 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    20881 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    20941 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    21001 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    21061 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    21121 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    21181 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    21241 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    21301 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    21361 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    21421 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    21481 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    21541 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    21601 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    21661 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    21721 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    21781 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    21841 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    21901 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    21961 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    22021 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    22081 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    22141 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    22201 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    22261 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    22321 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    22381 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    22441 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    22501 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    22561 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    22621 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    22681 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    22741 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    22801 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    22861 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    22921 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    22981 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    23041 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    23101 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    23161 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    23221 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    23281 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    23341 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    23401 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    23461 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    23521 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    23581 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    23641 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    23701 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    23761 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    23821 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    23881 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    23941 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    24001 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    24061 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    24121 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    24181 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    24241 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    24301 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    24361 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    24421 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    24481 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    24541 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    24601 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    24661 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    24721 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    24781 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    24841 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    24901 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    24961 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    25021 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    25081 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    25141 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    25201 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    25261 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    25321 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    25381 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    25441 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    25501 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    25561 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    25621 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    25681 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    25741 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    25801 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    25861 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    25921 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    25981 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    26041 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    26101 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    26161 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    26221 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    26281 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    26341 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    26401 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    26461 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    26521 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    26581 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    26641 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    26701 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    26761 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    26821 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    26881 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    26941 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    27001 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    27061 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    27121 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    27181 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    27241 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    27301 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    27361 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    27421 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    27481 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    27541 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    27601 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    27661 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    27721 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    27781 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    27841 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    27901 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    27961 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    28021 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    28081 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    28141 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    28201 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    28261 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    28321 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    28381 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    28441 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    28501 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    28561 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    28621 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    28681 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    28741 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    28801 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    28861 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    28921 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    28981 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    29041 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    29101 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    29161 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    29221 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    29281 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    29341 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    29401 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    29461 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    29521 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    29581 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    29641 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    29701 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    29761 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    29821 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    29881 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    29941 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    30001 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    30061 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    30121 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    30181 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    30241 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    30301 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    30361 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    30421 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    30481 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    30541 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    30601 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    30661 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    30721 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    30781 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    30841 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    30901 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    30961 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    31021 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    31081 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    31141 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    31201 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    31261 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    31321 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    31381 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    31441 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    31501 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    31561 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    31621 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    31681 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    31741 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    31801 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    31861 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    31921 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    31981 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    32041 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    32101 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    32161 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    32221 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    32281 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    32341 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    32401 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    32461 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    32521 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    32581 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    32641 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    32701 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    32761 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    32821 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    32881 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    32941 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    33001 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    33061 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    33121 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    33181 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    33241 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    33301 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    33361 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    33421 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    33481 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    33541 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    33601 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    33661 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    33721 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    33781 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    33841 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    33901 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    33961 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    34021 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    34081 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    34141 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    34201 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    34261 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    34321 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    34381 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    34441 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    34501 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    34561 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    34621 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    34681 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    34741 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    34801 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    34861 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    34921 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    34981 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    35041 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    35101 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    35161 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    35221 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    35281 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    35341 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    35401 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    35461 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    35521 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    35581 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    35641 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    35701 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    35761 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    35821 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    35881 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    35941 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    36001 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    36061 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    36121 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    36181 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    36241 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    36301 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    36361 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    36421 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    36481 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    36541 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    36601 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    36661 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    36721 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    36781 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    36841 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    36901 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    36961 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    37021 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    37081 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    37141 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    37201 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    37261 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    37321 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    37381 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    37441 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    37501 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    37561 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    37621 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    37681 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    37741 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    37801 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    37861 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    37921 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    37981 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    38041 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    38101 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    38161 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    38221 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    38281 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    38341 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    38401 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    38461 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    38521 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    38581 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    38641 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    38701 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    38761 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    38821 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    38881 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    38941 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    39001 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    39061 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    39121 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    39181 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    39241 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    39301 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    39361 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    39421 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    39481 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    39541 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    39601 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    39661 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    39721 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    39781 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    39841 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    39901 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    39961 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    40021 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    40081 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    40141 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    40201 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    40261 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    40321 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    40381 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    40441 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    40501 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    40561 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    40621 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    40681 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    40741 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    40801 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    40861 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    40921 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    40981 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    41041 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    41101 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    41161 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    41221 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    41281 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    41341 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    41401 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    41461 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    41521 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    41581 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    41641 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    41701 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    41761 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    41821 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    41881 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    41941 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    42001 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    42061 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    42121 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    42181 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    42241 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    42301 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    42361 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    42421 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    42481 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    42541 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    42601 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    42661 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    42721 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    42781 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    42841 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    42901 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    42961 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    43021 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    43081 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    43141 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    43201 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    43261 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    43321 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    43381 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    43441 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    43501 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    43561 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    43621 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    43681 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    43741 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    43801 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    43861 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    43921 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    43981 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    44041 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    44101 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    44161 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    44221 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    44281 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    44341 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    44401 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    44461 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    44521 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    44581 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    44641 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    44701 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    44761 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    44821 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    44881 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    44941 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    45001 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    45061 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    45121 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    45181 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    45241 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    45301 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    45361 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    45421 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    45481 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    45541 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    45601 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    45661 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    45721 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    45781 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    45841 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    45901 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    45961 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    46021 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    46081 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    46141 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    46201 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    46261 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    46321 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    46381 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    46441 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    46501 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    46561 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    46621 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    46681 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    46741 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    46801 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    46861 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    46921 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    46981 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    47041 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    47101 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    47161 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    47221 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    47281 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    47341 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    47401 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    47461 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    47521 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    47581 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    47641 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    47701 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    47761 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    47821 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    47881 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    47941 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    48001 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    48061 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    48121 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    48181 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    48241 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    48301 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    48361 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    48421 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    48481 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    48541 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    48601 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    48661 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    48721 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    48781 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    48841 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    48901 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    48961 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    49021 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    49081 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    49141 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    49201 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    49261 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    49321 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    49381 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    49441 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    49501 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    49561 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    49621 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    49681 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    49741 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    49801 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    49861 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    49921 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    49981 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    50041 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    50101 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    50161 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    50221 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    50281 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    50341 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    50401 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    50461 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    50521 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    50581 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    50641 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    50701 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    50761 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    50821 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    50881 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    50941 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    51001 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
//
LOCUS       DACCQP010000015        47470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCQP010000015 DACCQP010000000
VERSION     DACCQP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.015"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IVO87_000952"
     CDS             complement(55..789)
                     /locus_tag="IVO87_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7449167.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IVO87_000953"
     CDS             1116..1526
                     /locus_tag="IVO87_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7449168.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IVO87_000954"
     CDS             1519..2220
                     /locus_tag="IVO87_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7449169.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IVO87_000955"
     CDS             2220..3806
                     /locus_tag="IVO87_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7449170.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IVO87_000956"
     CDS             3825..4046
                     /locus_tag="IVO87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7449171.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IVO87_000957"
     CDS             4043..4804
                     /locus_tag="IVO87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7449172.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IVO87_000958"
     CDS             5184..5693
                     /locus_tag="IVO87_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7449173.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IVO87_000959"
     CDS             complement(5768..6307)
                     /locus_tag="IVO87_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7449174.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVO87_000960"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IVO87_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7449175.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IVO87_000961"
     CDS             7374..8171
                     /locus_tag="IVO87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7449176.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IVO87_000962"
     CDS             8199..8834
                     /locus_tag="IVO87_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7449177.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IVO87_000963"
     CDS             8854..9627
                     /locus_tag="IVO87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7449178.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IVO87_000964"
     CDS             10035..10832
                     /locus_tag="IVO87_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7449179.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IVO87_000965"
     CDS             10810..11223
                     /locus_tag="IVO87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449180.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IVO87_000966"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IVO87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7449181.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IVO87_000967"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IVO87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7449182.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IVO87_000968"
     CDS             16000..16560
                     /locus_tag="IVO87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7449183.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IVO87_000969"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IVO87_000969"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7449184.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IVO87_000970"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IVO87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7449185.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IVO87_000971"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IVO87_000971"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7449186.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IVO87_000972"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IVO87_000972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7449187.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IVO87_000973"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IVO87_000973"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7449188.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IVO87_000974"
     CDS             21907..22485
                     /locus_tag="IVO87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7449189.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IVO87_000975"
     CDS             22537..23019
                     /locus_tag="IVO87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7449190.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IVO87_000976"
     CDS             23136..23510
                     /locus_tag="IVO87_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449191.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="IVO87_000977"
     CDS             complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="IVO87_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7449192.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25687..26277
                     /locus_tag="IVO87_000978"
     CDS             25687..26277
                     /locus_tag="IVO87_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7449193.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26405..27754)
                     /locus_tag="IVO87_000979"
     CDS             complement(26405..27754)
                     /locus_tag="IVO87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449194.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27922..28662)
                     /locus_tag="IVO87_000980"
     CDS             complement(27922..28662)
                     /locus_tag="IVO87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7449195.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28675..30267)
                     /locus_tag="IVO87_000981"
     CDS             complement(28675..30267)
                     /locus_tag="IVO87_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7449196.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTVLASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30835..31131)
                     /locus_tag="IVO87_000982"
     CDS             complement(30835..31131)
                     /locus_tag="IVO87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449197.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31307..32032
                     /locus_tag="IVO87_000983"
     CDS             31307..32032
                     /locus_tag="IVO87_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7449198.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32025..32867
                     /locus_tag="IVO87_000984"
     CDS             32025..32867
                     /locus_tag="IVO87_000984"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7449199.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32870..33391
                     /locus_tag="IVO87_000985"
     CDS             32870..33391
                     /locus_tag="IVO87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7449200.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33644..34207)
                     /locus_tag="IVO87_000986"
     CDS             complement(33644..34207)
                     /locus_tag="IVO87_000986"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7449201.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34458..34727
                     /gene="rpsO"
                     /locus_tag="IVO87_000987"
     CDS             34458..34727
                     /gene="rpsO"
                     /locus_tag="IVO87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7449202.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34915..37041
                     /gene="pnp"
                     /locus_tag="IVO87_000988"
     CDS             34915..37041
                     /gene="pnp"
                     /locus_tag="IVO87_000988"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7449203.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37551..37817
                     /locus_tag="IVO87_000989"
     CDS             37551..37817
                     /locus_tag="IVO87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7449204.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37961..39952
                     /locus_tag="IVO87_000990"
     CDS             37961..39952
                     /locus_tag="IVO87_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7449205.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40306..41607
                     /locus_tag="IVO87_000991"
     CDS             40306..41607
                     /locus_tag="IVO87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7449206.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41737..43107)
                     /locus_tag="IVO87_000992"
     CDS             complement(41737..43107)
                     /locus_tag="IVO87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7449207.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43289..43612)
                     /locus_tag="IVO87_000993"
     CDS             complement(43289..43612)
                     /locus_tag="IVO87_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7449208.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43937..44545)
                     /locus_tag="IVO87_000994"
     CDS             complement(43937..44545)
                     /locus_tag="IVO87_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7449209.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44875..45591
                     /locus_tag="IVO87_000995"
     CDS             44875..45591
                     /locus_tag="IVO87_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7449210.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45604..46299
                     /locus_tag="IVO87_000996"
     CDS             45604..46299
                     /locus_tag="IVO87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7449211.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46383..47012
                     /gene="udk"
                     /locus_tag="IVO87_000997"
     CDS             46383..47012
                     /gene="udk"
                     /locus_tag="IVO87_000997"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7449212.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8788 c         9877 g        13413 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat
    23881 aaaagaaatg ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt
    23941 atatggagct actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa
    24001 taaacggtgt tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga
    24061 agccattttc aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc
    24121 aataaacagt gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc
    24181 tcttttatca atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa
    24241 aaccgataac tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt
    24301 ccgcaacata taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt
    24361 tcaacccatc aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg
    24421 tacgctgctt aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat
    24481 agatgaacat caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg
    24541 ctaaacaagg aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca
    24601 aatctgtgaa tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta
    24661 atataacgat aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct
    24721 gagccagcgt gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta
    24781 aataagcgag caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga
    24841 tcgtgtcttt agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa
    24901 tgatgatccc aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc
    24961 ctgtgaaaaa agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta
    25021 gtggattcaa aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca
    25081 ggaactttcc aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca
    25141 caaaaaatac aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg
    25201 gtgctaaacc aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga
    25261 taactaagta aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta
    25321 attcatatac acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac
    25381 cgataccggt aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac
    25441 ccaaatgaac gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc
    25501 ctataaaaag atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt
    25561 ttttaaaagt aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa
    25621 aacgacggtt atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga
    25681 atcatcgtga caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg
    25741 agagttcttg tattagagat caagatccct aatacatcta gctttcattc tttatgggtc
    25801 atccgaaata cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg
    25861 aaccatcatc gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat
    25921 atctttttgc tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac
    25981 cctgaagctt tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct
    26041 tttggtatct tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga
    26101 gttttgtcaa aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc
    26161 ggtgtctttt ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg
    26221 attttttcct ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa
    26281 agaggctggg acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa
    26341 gaagcaattt tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac
    26401 tttcttactt cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca
    26461 caggtgttag taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa
    26521 aggccatgta aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat
    26581 gagctttttt gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc
    26641 agataaacaa gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca
    26701 tcacgtaatt caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc
    26761 ctggcacatg atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta
    26821 aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac
    26881 ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt
    26941 ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa
    27001 aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca
    27061 acactttttc aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa
    27121 aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc
    27181 cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga
    27241 ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa
    27301 accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac
    27361 ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca
    27421 ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc
    27481 cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt
    27541 tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc
    27601 cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac
    27661 ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt
    27721 tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt
    27781 ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc
    27841 agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt
    27901 tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg
    27961 ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt
    28021 atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat
    28081 aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat
    28141 ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa
    28201 tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc
    28261 gatataacga tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc
    28321 tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt
    28381 gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt
    28441 atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc
    28501 cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt
    28561 cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa
    28621 gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc
    28681 ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt
    28741 cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc
    28801 gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa
    28861 gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa
    28921 ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat
    28981 cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc
    29041 aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa
    29101 caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat
    29161 gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag
    29221 tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg
    29281 gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa
    29341 caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa
    29401 atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc
    29461 ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt
    29521 ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt
    29581 ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc
    29641 cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag ctaaaactgt
    29701 tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat
    29761 cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa
    29821 actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta
    29881 ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat
    29941 gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac
    30001 gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc
    30061 gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga
    30121 ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt
    30181 ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac
    30241 ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata tgatacacga
    30301 gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata
    30361 gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa
    30421 cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca
    30481 agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac
    30541 tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag
    30601 ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt
    30661 ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat
    30721 gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca
    30781 ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc
    30841 tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat
    30901 ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata
    30961 tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca
    31021 aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa
    31081 ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca
    31141 cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg
    31201 ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat
    31261 aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac
    31321 agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc
    31381 aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg
    31441 gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt
    31501 ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga
    31561 aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt
    31621 gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca
    31681 ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca
    31741 agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg
    31801 agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac
    31861 gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat
    31921 cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga
    31981 cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa
    32041 ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg
    32101 ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac
    32161 tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc
    32221 cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt
    32281 tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc
    32341 cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc
    32401 tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg
    32461 tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca
    32521 aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag
    32581 aggaagaact aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc
    32641 gagagttgat tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga
    32701 aaaaagaaga aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag
    32761 ttgttagtct gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt
    32821 tgatgaaaca ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg
    32881 agagacacgg cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc
    32941 tagcaagttt gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt
    33001 actacgagct gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga
    33061 agaaaccaat actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga
    33121 agaagaactg agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca
    33181 tcccctttac ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc
    33241 ctttatgaga cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat
    33301 ccatctgcat acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact
    33361 agctgaagca ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa
    33421 actattctat catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa
    33481 gatgcgtatc caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt
    33541 tcgattagtg gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat
    33601 aaagctttct tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata
    33661 cgccttcttt tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt
    33721 tgatatggtc gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt
    33781 gtttttcacc attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc
    33841 ctggtacttc tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa
    33901 cagaatggct caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct
    33961 ctgggtcagg acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag
    34021 cagctaagcc aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt
    34081 tttttaagaa ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag
    34141 aaacttcttt tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg
    34201 taatcatgga atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta
    34261 tagtttacca caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa
    34321 tgattaacta cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca
    34381 catgacctgc cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat
    34441 cgaaagaggt gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat
    34501 acgctcgcca tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag
    34561 aaatcaacca cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg
    34621 gcctaatgaa aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg
    34681 ttcaacgtta ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa
    34741 gagtgggatt cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt
    34801 gatcaagcta gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc
    34861 agttttcatt tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca
    34921 gaaaaacaag tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag
    34981 ctagcaaaac aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt
    35041 gcagctgtgg cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat
    35101 gaagaaaaaa tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt
    35161 ccaagtacgg aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt
    35221 gcagaaggct tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac
    35281 tgctcgccag ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc
    35341 ccatttgacg gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg
    35401 aatccaacag tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa
    35461 caagccatca atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt
    35521 gcattgctgt ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt
    35581 caagcagtag ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa
    35641 aaagaaatct ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa
    35701 cttgctcgcg aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag
    35761 aaatttgcag gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa
    35821 gatcttgaaa aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg
    35881 cgtaaattgg atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat
    35941 ggatcaggtt tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct
    36001 ctaggagagc accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac
    36061 cactacaatt tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt
    36121 cgggaaatcg gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa
    36181 gaagaatttc cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct
    36241 tcccaagcaa gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa
    36301 gcgccagtcg ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta
    36361 actgatatcc aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca
    36421 aaagatggta tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc
    36481 ctaactgaag cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact
    36541 tcaacgattg ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc
    36601 cagatcgaac ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga
    36661 atcatcgatg aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct
    36721 tcagctgatg cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa
    36781 gtcaaagttg gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc
    36841 gtcaacttga tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt
    36901 gtcaataaag tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa
    36961 atcgataaac aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca
    37021 acgaaaaatg aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt
    37081 gacaggggga caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc
    37141 ttttttttcg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    37201 taaaaacatt aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt
    37261 tttacaacct ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa
    37321 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg
    37381 atttcacaac ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca
    37441 cttttgttac aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg
    37501 aataaagcgt aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt
    37561 ttttatggtc gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa
    37621 aggaagtccc gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg
    37681 gcgaattttt actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat
    37741 tattgggtgc aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac
    37801 gtccaaagaa agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa
    37861 ttagaagcaa tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt
    37921 acctaaagga tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac
    37981 caaaaagata tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg
    38041 gacaagtccg ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat
    38101 caatcctgat tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac
    38161 aacaatcaaa tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt
    38221 ctcactctac acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg
    38281 aggagctgcc ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat
    38341 tgaaggcttg cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat
    38401 cgtcattatc actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga
    38461 tattgtcgga aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat
    38521 gggattaatg aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc
    38581 tatccagctg ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt
    38641 cctggctacg acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa
    38701 tattcgtctg agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc
    38761 tgcgtggttg ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc
    38821 attcttccaa atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc
    38881 tgcagtcatt gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat
    38941 caagttgaaa ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat
    39001 gtatatccca gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt
    39061 ccgaacgtct catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat
    39121 gacaacagca ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata
    39181 tttagttacc tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa
    39241 attcttccga ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt
    39301 tatttgggaa caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga
    39361 ttatatcccg cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa
    39421 tgtggtcttc ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc
    39481 gattttagat aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac
    39541 ggatgagcca tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca
    39601 agtgaatctg ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt
    39661 catccatgat ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac
    39721 accagggcga gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt
    39781 gacaactcta ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat
    39841 cgctacctcg ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt
    39901 tccgcttatc attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg
    39961 ctttgacctg aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga
    40021 aaaagctgta ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt
    40081 catctgtttg ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc
    40141 atgaaaaagc tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc
    40201 tgaaaaatgc cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt
    40261 tttgtagaaa aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc
    40321 attattttat ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta
    40381 gtgaaaatca gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag
    40441 ctagctcttc atgttacaca ccatttagat aactatttat cagctagtca attaggaatt
    40501 acgctaaccg gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa
    40561 ccagtgatcg gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg
    40621 ggatttattt tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat
    40681 agtatcgtca atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat
    40741 aaagtaatgt ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta
    40801 ttaggtgtgc gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta
    40861 gtcgcattga attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa
    40921 aacgtgtttg aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg
    40981 caagtattcg aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga
    41041 catactcgct atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg
    41101 ccagatttga tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa
    41161 gagccgatcg taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa
    41221 aagggtaagc atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg
    41281 attgaagata ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa
    41341 gcattgatag cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat
    41401 gatttccagc gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca
    41461 cttgcgggct ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt
    41521 gcatcattcc gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta
    41581 gagtccacgg aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc
    41641 ctttttgtta tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt
    41701 ttcattttta gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa
    41761 tgtgaaatgt tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta
    41821 aagcaacacg gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta
    41881 tccccacatt cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc
    41941 ggcgatcgta gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag
    42001 gactgctaag atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa
    42061 tacacgtgtt tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac
    42121 gatagcacct gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg
    42181 caagattcgg ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat
    42241 accaaatgac atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc
    42301 agctacgaca tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc
    42361 gatgatcgaa gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc
    42421 tttttcaaat ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata
    42481 cactagacac agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc
    42541 cacacccatg ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc
    42601 aatgatcaga aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag
    42661 caaattgaaa ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc
    42721 ttgatcaagt atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag
    42781 acttaggaca gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact
    42841 tttcttcaga ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc
    42901 agctgttaaa ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac
    42961 cgctgccagc gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta
    43021 gaactgctga aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc
    43081 ggggcttcct tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt
    43141 agtagtatat agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac
    43201 tccataaaaa gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa
    43261 taagggacat ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga
    43321 tcgtttctct cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg
    43381 caaacggcaa atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca
    43441 cttttccacg attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct
    43501 taagatctag gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata
    43561 cgacaccaac tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa
    43621 cctccttcga atcaacgaat gttttatttt tataattaat cctaggataa actagaaata
    43681 cttacttcaa cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat
    43741 actttttttc aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt
    43801 ggtaaattag gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa
    43861 taaacgatta gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca
    43921 cctgattgtt tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac
    43981 ctgtgctttc ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg
    44041 tcacgaattt gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt
    44101 ccaagtttgt cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga
    44161 ctactgtata caagttttga gctaacactc caccagattt agcacccact gattcgccaa
    44221 caagaacgac ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg
    44281 catgttcgaa atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt
    44341 tttgtccatc gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc
    44401 ttagtgtcaa accatcgaat gagatagggg tactttctac tttataccca taaacaacag
    44461 ctggaacttt cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt
    44521 tacttacttc taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct
    44581 ttggtaacgg acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg
    44641 aaggcagggc gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc
    44701 aggattcaac ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta
    44761 ctatttaacc gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg
    44821 caagcaagaa tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt
    44881 ttagataaat taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt
    44941 gctttgaagc aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat
    45001 agtttactgc atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat
    45061 cttctcaata aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta
    45121 atcgatctga ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat
    45181 agagatacaa caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta
    45241 cttccagaaa aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg
    45301 gcagacgtaa cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg
    45361 gctgatttaa aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa
    45421 gaaggaaaat tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca
    45481 ttgaaacggc taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat
    45541 cgatcgttga cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa
    45601 aagatgaaat ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta
    45661 actgaagcca atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa
    45721 atcgaaacca gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga
    45781 ctatcacgag aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa
    45841 acagtagata gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat
    45901 cttgttccag gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt
    45961 gtgacgaacg gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat
    46021 tacttcaagg atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt
    46081 tttgatcatg tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt
    46141 gattctttga cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc
    46201 aatagaaata aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta
    46261 gaggaacttt attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt
    46321 ttcgtgtttt ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt
    46381 caatgacaaa aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga
    46441 caagtgtcag ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac
    46501 aagattctta ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt
    46561 acgatcaccc atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga
    46621 actataaagc cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa
    46681 cgatcattca ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg
    46741 aacgtctgcg tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta
    46801 tcattcgtcg tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg
    46861 aacaatattt gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct
    46921 atgcggatat cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa
    46981 caaaagtagc tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc
    47041 gaccgtttga aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat
    47101 ttataacaag aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa
    47161 aatagctgtt tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac
    47221 cgtgaagtag aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa
    47281 aacagctcct cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac
    47341 accgtttaac gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac
    47401 gaaaattatt tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg
    47461 tcgcaccccc
//
LOCUS       DACCQP010000016        46480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCQP010000016 DACCQP010000000
VERSION     DACCQP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.016"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..680
                     /locus_tag="IVO87_000998"
     CDS             <1..680
                     /locus_tag="IVO87_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7449213.1"
                     /translation="SFQVKERQHQESLQLYQINEEYYHHLEEFKHDYKSLLFSLKITL
                     QENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQV
                     VIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKAN
                     PTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLAS
                     RKSKSAY"
     gene            1002..1229
                     /locus_tag="IVO87_000999"
     CDS             1002..1229
                     /locus_tag="IVO87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7449214.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLMHTHGGYLNY"
     gene            1352..1678
                     /locus_tag="IVO87_001000"
     CDS             1352..1678
                     /locus_tag="IVO87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7449215.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1746..2459
                     /locus_tag="IVO87_001001"
     CDS             1746..2459
                     /locus_tag="IVO87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7449216.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2769..3619
                     /locus_tag="IVO87_001002"
                     /pseudo
     CDS             2769..3619
                     /locus_tag="IVO87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            3740..5605
                     /locus_tag="IVO87_001003"
     CDS             3740..5605
                     /locus_tag="IVO87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449217.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5653..7062
                     /locus_tag="IVO87_001004"
     CDS             5653..7062
                     /locus_tag="IVO87_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7449218.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7170..7871
                     /locus_tag="IVO87_001005"
     CDS             7170..7871
                     /locus_tag="IVO87_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7449219.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            8030..8509
                     /locus_tag="IVO87_001006"
                     /pseudo
     CDS             8030..8509
                     /locus_tag="IVO87_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            9356..9931
                     /locus_tag="IVO87_001007"
     CDS             9356..9931
                     /locus_tag="IVO87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7449220.1"
                     /translation="MKFTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            9931..10371
                     /locus_tag="IVO87_001008"
     CDS             9931..10371
                     /locus_tag="IVO87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7449221.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            10387..11088
                     /locus_tag="IVO87_001009"
     CDS             10387..11088
                     /locus_tag="IVO87_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7449222.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(11576..12001)
                     /locus_tag="IVO87_001010"
     CDS             complement(11576..12001)
                     /locus_tag="IVO87_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449223.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            12366..13565
                     /locus_tag="IVO87_001011"
     CDS             12366..13565
                     /locus_tag="IVO87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449224.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            13587..13799
                     /locus_tag="IVO87_001012"
     CDS             13587..13799
                     /locus_tag="IVO87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7449225.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            13874..14350
                     /locus_tag="IVO87_001013"
     CDS             13874..14350
                     /locus_tag="IVO87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7449226.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            14347..16155
                     /locus_tag="IVO87_001014"
     CDS             14347..16155
                     /locus_tag="IVO87_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7449227.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            16232..16447
                     /locus_tag="IVO87_001015"
     CDS             16232..16447
                     /locus_tag="IVO87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7449228.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            16539..17402
                     /locus_tag="IVO87_001016"
     CDS             16539..17402
                     /locus_tag="IVO87_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449229.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            17433..17975
                     /locus_tag="IVO87_001017"
     CDS             17433..17975
                     /locus_tag="IVO87_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7449230.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            17989..18411
                     /locus_tag="IVO87_001018"
     CDS             17989..18411
                     /locus_tag="IVO87_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7449231.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            18341..20740
                     /locus_tag="IVO87_001019"
     CDS             18341..20740
                     /locus_tag="IVO87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7449232.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            20772..22763
                     /locus_tag="IVO87_001020"
     CDS             20772..22763
                     /locus_tag="IVO87_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7449233.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            22786..23037
                     /locus_tag="IVO87_001021"
     CDS             22786..23037
                     /locus_tag="IVO87_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7449234.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            23027..24256
                     /locus_tag="IVO87_001022"
     CDS             23027..24256
                     /locus_tag="IVO87_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7449235.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            24253..26334
                     /gene="topB"
                     /locus_tag="IVO87_001023"
     CDS             24253..26334
                     /gene="topB"
                     /locus_tag="IVO87_001023"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7449236.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            26483..30403
                     /locus_tag="IVO87_001024"
     CDS             26483..30403
                     /locus_tag="IVO87_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7449237.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITKIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            30404..31348
                     /locus_tag="IVO87_001025"
     CDS             30404..31348
                     /locus_tag="IVO87_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449238.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            31352..31555
                     /locus_tag="IVO87_001026"
     CDS             31352..31555
                     /locus_tag="IVO87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449239.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(31855..32091)
                     /locus_tag="IVO87_001027"
     CDS             complement(31855..32091)
                     /locus_tag="IVO87_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449240.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(32274..32645)
                     /locus_tag="IVO87_001028"
     CDS             complement(32274..32645)
                     /locus_tag="IVO87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449241.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(32642..33970)
                     /locus_tag="IVO87_001029"
     CDS             complement(32642..33970)
                     /locus_tag="IVO87_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7449242.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(33931..34260)
                     /locus_tag="IVO87_001030"
     CDS             complement(33931..34260)
                     /locus_tag="IVO87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7449243.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(34518..34889)
                     /locus_tag="IVO87_001031"
     CDS             complement(34518..34889)
                     /locus_tag="IVO87_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449244.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            35034..35219
                     /locus_tag="IVO87_001032"
     CDS             35034..35219
                     /locus_tag="IVO87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7449245.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            35731..35949
                     /locus_tag="IVO87_001033"
     CDS             35731..35949
                     /locus_tag="IVO87_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449246.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            36219..37025
                     /locus_tag="IVO87_001034"
     CDS             36219..37025
                     /locus_tag="IVO87_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7449247.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            37088..38059
                     /locus_tag="IVO87_001035"
     CDS             37088..38059
                     /locus_tag="IVO87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7449248.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            38052..39080
                     /gene="vanB"
                     /locus_tag="IVO87_001036"
     CDS             38052..39080
                     /gene="vanB"
                     /locus_tag="IVO87_001036"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7449249.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAVCMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            39086..39694
                     /locus_tag="IVO87_001037"
     CDS             39086..39694
                     /locus_tag="IVO87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7449250.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            40282..40713
                     /locus_tag="IVO87_001038"
     CDS             40282..40713
                     /locus_tag="IVO87_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7449251.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            40720..40953
                     /locus_tag="IVO87_001039"
     CDS             40720..40953
                     /locus_tag="IVO87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449252.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(40875..41117)
                     /locus_tag="IVO87_001040"
     CDS             complement(40875..41117)
                     /locus_tag="IVO87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449253.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            41374..41577
                     /locus_tag="IVO87_001041"
     CDS             41374..41577
                     /locus_tag="IVO87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7449254.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            41661..42854
                     /locus_tag="IVO87_001042"
     CDS             41661..42854
                     /locus_tag="IVO87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7449255.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            43294..44265
                     /locus_tag="IVO87_001043"
     CDS             43294..44265
                     /locus_tag="IVO87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7449256.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            44297..45694
                     /locus_tag="IVO87_001044"
     CDS             44297..45694
                     /locus_tag="IVO87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449257.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            45853..46476
                     /locus_tag="IVO87_001045"
     CDS             45853..46476
                     /locus_tag="IVO87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449258.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDSDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        13107 a        10572 c        11789 g        11012 t
ORIGIN      
        1 tcagtttcca agtaaaagaa agacaacatc aggaatcgct tcaactgtat caaataaatg
       61 aagaatatta ccatcatcta gaggaattca aacatgatta taaaagtcta ctgttttcat
      121 taaaaattac tcttcaggaa aataaaaaag aaacgttgga atttttagaa atattagaag
      181 atcattctgc agctattttt gaaaaaccgc aaatacaaca gctaacaaat attgtatccc
      241 cagctgtcag gggtgtcttt ttaaaatttc tagaaaaagc agaatcggag aaaatcccga
      301 tccaagtagt tattcctgtc gaagtgaaaa caatcccgat tgatctggtt gtttttattc
      361 gctgtttatc tatttttatt tctaacgcat tcgatcatcg tgtagccgat cagagtccga
      421 ttacgatcaa tctgctggaa gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa
      481 atccaacgaa tttgacgcta tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg
      541 gcgggttagg tctatacagt gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa
      601 aaatcgaatt ttcaaaacag aatcactatt tttttgtaga aatgtatctg gcatctagaa
      661 agagcaaatc agcctactga cttactgtca aaggataagt ttgaggaaag tctacacgga
      721 gatagagaca acaacacagc atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc
      781 ttctgtttct tttgctatgt agttaaacaa agtagttgac acttccgcta cttagtatta
      841 gtacgaaagt atacttcatg atactaagta gataagaagt gaaaagcgga acccacgatc
      901 gtcaaaatgc taactgcttt gcttacacca gataaaggga agagcaatat cgcttatttt
      961 aggcgtggag aagcaagcaa atccctacga tcacaaaaaa aatgctgacc cagcagttaa
     1021 gagagttaga acaagatgaa agtatccatc gaaaagccta taaccaagtc ccgcctaaag
     1081 tagagtactc gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgttgtctg
     1141 tttgggaaga agaacgtatc gaaaaacaaa aagcctcaag ggaagaagac acgctaatgc
     1201 atacacatgg tggctaccta aattattgat tttctatgac atttatttta aataaaagaa
     1261 aacaggagtt tttttaaaag catcatctat tttttatgta aaaaaatggt atgattaatc
     1321 aagtaagaag tgattggaag aaggcaagca aatgaatagt atggaaaaag tcaaccagtt
     1381 tagagatgaa cgtaattggc gacaatttca taacgaaaaa gatttggcta tctctatttc
     1441 tttggaagca tctgaattgt tagaactttt tcaatggaaa acgccggaag aagtaaaaga
     1501 aacacaatta gaacgaatca aagaagaact ggcagatgta ttgatctatt catacatgat
     1561 ggcagacaac cttcacctgg atctagatga gatcattgaa gaaaaattga tcaaaaataa
     1621 tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa
     1681 atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag taaaatgagt ctggagggag
     1741 agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt caatatcctc ttgttcctct
     1801 tgttattagt aggactagct ttgattttca atgaacagat caaggattat tttgtcagag
     1861 agacaggaga taaatatgct atagcgaatg taacaaaaga agatctgaaa aagaataacg
     1921 ataaagatgt cagttttgac tttgatgcgg tagaaccaat gacgacagaa ggggtcatga
     1981 ggtcgcaaat gagaggaaca gatctaccag tgattgctag tatcgcagtc ccttcagttt
     2041 caatcaattt accggttttc aaaggcttgg ataatacgtc actactttac ggagcaggaa
     2101 cattatctcc agatcaagaa atgggaaaag gaaattatgc cttagcaagc catcgtgcaa
     2161 cgaaccctga attgctgttt acaccgctag aaaatttaga gatgggtgcc aagatctatt
     2221 tgacagattt agaaaatgtg tatacataca aaacattttt taaagaaaaa gttgctccta
     2281 cagataccca attactgaat gaagtcgagg gaaaagaaat tgtcacacta attacttgtg
     2341 gcgacatgga cgccgtcacg cgactagttg tacaaggaga actggaaagt gttacatcga
     2401 taaaagatgc gacagatgat atgagatctg ctttcaatct agagacaaaa acattttagg
     2461 atactgggac ataagtcgtt acagattaaa aacagccaaa ggatatgaat cgataaggaa
     2521 aaaccaattt gctgaaaaaa agagaaacgc aaagaggtgc atctctgatt tggttgtttt
     2581 ctagatagac ttgtgttcca gtttttttat tttttatatg aaaaggcttg cataaataaa
     2641 aattcgatgg tatgatgatt ttgaataaga aatggattgt tactgtacgg caggcaaaac
     2701 cagaattcag ctaagaaaca gtaggcgaat tctaggtttt tttatttttc ttgagagggc
     2761 gaagcaaaat gaaaattgat aaaattttga ataacaatgt cgtcatttcg aaaaatggat
     2821 tcggggaaga agtcgtttgt atgggaaaag gactagcttt tcaaaaaaag attggggacg
     2881 agatttcgcc agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc
     2941 aattccagca gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag
     3001 tcgaactggc tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga
     3061 ccgatcatct tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat
     3121 tattatttga aacaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt
     3181 gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt
     3241 tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac
     3301 aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga
     3361 atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa
     3421 aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc
     3481 aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga
     3541 aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa
     3601 aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa
     3661 taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa
     3721 aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt
     3781 aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt
     3841 aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt
     3901 cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca
     3961 atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa
     4021 tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat
     4081 ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac
     4141 agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt
     4201 acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt
     4261 agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc
     4321 tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat
     4381 accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat
     4441 tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt
     4501 aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt
     4561 caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca
     4621 agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga
     4681 acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg
     4741 ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc
     4801 ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc
     4861 tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg
     4921 attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt
     4981 cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat
     5041 tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga
     5101 caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac
     5161 cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt
     5221 ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc
     5281 acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac
     5341 gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa
     5401 tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg
     5461 ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac
     5521 gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga
     5581 agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa
     5641 aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac
     5701 ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc
     5761 ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga
     5821 ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc
     5881 aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact
     5941 cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat
     6001 attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac
     6061 ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta
     6121 gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt
     6181 cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat
     6241 attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac
     6301 gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc
     6361 gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc
     6421 tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa
     6481 tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa
     6541 tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat
     6601 ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag
     6661 gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta
     6721 tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat
     6781 atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc
     6841 gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga
     6901 tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat
     6961 gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat
     7021 tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa
     7081 aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt
     7141 ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag
     7201 tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc
     7261 cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa
     7321 tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc
     7381 taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga
     7441 actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg
     7501 aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt
     7561 tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca
     7621 gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta
     7681 ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc
     7741 tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg
     7801 aaatgattga atattcggag gcggtctacc atacggagct gcatccttac catattgatt
     7861 atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     7921 agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca
     7981 cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg
     8041 aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac
     8101 gatcgcttat ttcttaggga aaatcattcg taaaaatata ttcaatcgtc aagaaatcga
     8161 tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat
     8221 cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtgggg cagtaggtgg
     8281 cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt
     8341 cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac
     8401 aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga
     8461 agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa
     8521 tggattacaa gaaaaaagta caatagatgt accttgaggc aggaatggta tgaaagaatc
     8581 agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag
     8641 catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaaa
     8701 ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa
     8761 aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt
     8821 acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca
     8881 ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc
     8941 gttaacaaaa agatttttag gataagctga tgaacaaaaa tgatcaaatt ttgatcgttt
     9001 agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac
     9061 aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta
     9121 ttggcatata ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa
     9181 acacgttatt tatttttgtt ttatctataa aatagctgaa aaattatttt ttctaaatac
     9241 tatatatagt ggtgtctatc ctatatacca ctaaaaaact acaaaatcta gtcttttatt
     9301 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
     9361 atttactgtt ttttgcggtt cccgttttgg taataaagaa agttacaaat ttattgcgca
     9421 aacgttagga aaatacatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
     9481 cgggattatg ggagtgatta gtaaaacagt actggaaaat aatggaaaag tcacaggtat
     9541 ctatccgaat gggctttttg aaactgaatt gcctggaaaa gatgttacta catttattcc
     9601 tacagaaacg atcgatgaaa gaaaggtatt attatttgaa aagggagatg ctgtcctcgt
     9661 tttccctgga ggtctaggta cattggagga attctcacaa cttctttctt ggatagcaat
     9721 cggtctgacg cctgacaagc caatcggaat attgaatatt ggcggttatt acagcggctt
     9781 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
     9841 agtaatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
     9901 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
     9961 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    10021 acagtcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    10081 gctggatggg cgcaggaagt atacgaaaaa gatatggaag aactgacaaa tgcagaattt
    10141 gttgttgcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    10201 ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac aagaagagac ggtacctacg
    10261 aacttgatga ttacacaaag tctccatacg tatttaaaat cagaccaagc tgtacgtgaa
    10321 tatgattttg aaacattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    10381 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    10441 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    10501 gtattgccgc tcttttattc aaatcctgag cagtacgctt ttttattgca aatcttcttt
    10561 ttgaacaagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    10621 cgatccatct atgaagatag cctgttgttc catctaaacg ctgatttagg tcgggtaacg
    10681 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    10741 gttgctccaa aaaaacggcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    10801 ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc tagaaagcga tgagacgtta
    10861 tatacctatt atgaaacgtt gaacacccgc tataatcaat ggtatgaaga ttttgatgta
    10921 tgtcctaaaa tacaagtgga tggggataaa ttcgattttg tagaaaatcc agaagatgca
    10981 aaaaaggtga tccatcagat ccaagaaaaa ctagctgaat tggaaggaaa gcctgctgga
    11041 aaatatttta cggataacca gcatgcaaaa ggtcaagaat taggctaaaa ataaaaaaac
    11101 aaacgacggg aaagaaaaat tactacgtga tgtagaataa gcctttctat cgtttggtta
    11161 ttttatattc aaatatttga aataaataaa tctttactct ttgatttgta aactgaaatg
    11221 atccactgtt tgttttactg atgaagcttg atcaaaggaa gaagctgagt ttgaagttca
    11281 taaaaatcag ccatagtgag ttttgcttgg atttgtttat cttcttctac tttttacata
    11341 attttgaaca ggataagatt caaatgtttt aaatgaatct ttgactagtt tgtgacagtt
    11401 gtcgcatatg ctcaagcttc cttggtcatt aaattctgta tggatatttt tgttgttatt
    11461 acatgtacaa taatcaggac tatcgatcat ccatttcgtc tcctttacaa tcatcttgct
    11521 tctttactat agcagaaaag aaggggagaa acaaaagaag atccaacaga cggcactata
    11581 gactgtagat gttgccatcc tcatccttca aacggtaatt accgattttg gtaaatgtga
    11641 tggttgcttc ttttttattt tcttttcctg aaataatata ttcgtcatct ttggtagtaa
    11701 tcttgatgtc tgtcagcact gttttttctt cagatgattc agaagaatca gtttcttgtg
    11761 tctgatacgt gcgtgtccat tccatttttt tatctgtaaa tgtcaactgt cccgcggata
    11821 aatccatatc tttacgtact tttagcgtat atacactgtt agtgatatct gctttttccg
    11881 cttttaagac agaagaatcg gttgtttcat ccgaggattg gtcagagccg ccaaacaaat
    11941 tacagccagt caatgtcaaa agtcccgcag acaaaataaa tagtacgatt aatttcttca
    12001 aaatacttcc cccatttgat aggataaaga aattatacca ttggaaagta aaacttcaaa
    12061 cggatggaag agaaaaacgc acaaaacacc cacaaataga gtaaatttta tttgaaaatt
    12121 ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga gaactcaatt
    12181 tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata ctgctaatac
    12241 ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg cggcgttcct
    12301 ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag aaaggaggcg
    12361 acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg caggaaaagc
    12421 ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc
    12481 gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt ccctatcagc
    12541 cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg aacaacgcta
    12601 tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat caccacctgc
    12661 ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg tccttgccgg
    12721 aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc atcgacgcga
    12781 tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag cagggccgtg
    12841 acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg gtattggaaa
    12901 acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg acatcggata
    12961 atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact gacttatcaa
    13021 aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc ccatccccga
    13081 cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa aggacaggaa
    13141 aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag tatgcctatc
    13201 tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac ctgatgctgg
    13261 aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac cctgccgagc
    13321 tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc cttgactgtc
    13381 tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct atgctgttca
    13441 acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac atggcacagc
    13501 ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag ggcgaaagcc
    13561 tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt gacgagcgaa
    13621 cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg cattgcgggg
    13681 agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa atggccgaaa
    13741 actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg cggatttgac
    13801 ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga ctttctgaca
    13861 aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt ccatgaaaac
    13921 gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc aacaccgggc
    13981 caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta aaaagcatgg
    14041 ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca agccatgcgc
    14101 caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc cgccccacta
    14161 cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc gggagtttac
    14221 cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt ccgctgtgaa
    14281 gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg aaagggggct
    14341 ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta tctgattttt
    14401 gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc tgacctgtcg
    14461 gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc gctgccaagc
    14521 ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg gctggtggtg
    14581 tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc
    14641 tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa taatatcctg
    14701 ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa gacagcgcgg
    14761 aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa
    14821 cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt ggtgacagat
    14881 ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt
    14941 aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt tgtctacatc
    15001 cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac caagggcgag
    15061 ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta ttcggccctg
    15121 atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac gctgctggaa
    15181 atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc
    15241 atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca gtacaagaaa
    15301 ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg tgccagactt
    15361 gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat ggagctggac
    15421 accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga cacgaccttt
    15481 aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga ccgggctgat
    15541 gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga atttgccaac
    15601 attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg tgaaatatcg
    15661 gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga cgccgccgag
    15721 atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag
    15781 gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag cgagaaccgg
    15841 ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt gatgtcccaa
    15901 gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg
    15961 tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct tgccgacgcc
    16021 gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg gaaacggcag
    16081 caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac
    16141 gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa aaatcattat
    16201 gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg cagttactac
    16261 tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg gcatcaatct
    16321 gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca agcagcttgt
    16381 cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt ccgggctgct
    16441 gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc gcgtcctccc
    16501 ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa atcattgaag
    16561 aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg ctgttcgata
    16621 cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg caggcatgga
    16681 acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg
    16741 gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg gaaaagaaca
    16801 atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc gcgtttgcaa
    16861 tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc acccaaagcg
    16921 tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt aacaccctga
    16981 taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg gggctgtggt
    17041 tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt ttccttgtga
    17101 cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc ccccttgcca
    17161 caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc
    17221 tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg ctgatacaga
    17281 atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc tatacggtgc
    17341 tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc ggggcgcatt
    17401 gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac aacatcattt
    17461 acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg gaaaatcact
    17521 attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag
    17581 actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg cggttaatca
    17641 aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa
    17701 gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa atctgcctgc
    17761 tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc
    17821 cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta gagctggcgg
    17881 gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg
    17941 ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat ggcttatgta
    18001 accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg
    18061 caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt tctcacccgt
    18121 gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct gccgggcttt
    18181 ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat gcagatcatc
    18241 cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc
    18301 cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc
    18361 aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg acgcggtaat
    18421 ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc cctacgaggt
    18481 catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt gcattgagtt
    18541 tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct ttgaaaaact
    18601 gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt tcctcaatcg
    18661 gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg gggacgactt
    18721 cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa acggcaacaa
    18781 cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg tgaaagcggc
    18841 gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga gcatgggggc
    18901 cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc
    18961 ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc tctccaccaa
    19021 ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg gcatgggcgg
    19081 caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg acgatatgct
    19141 ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc aggccattga
    19201 acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg ctatgaagat
    19261 tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc ccagcgacct
    19321 tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg
    19381 gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa agctgaataa
    19441 cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg tccggctgga
    19501 ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat
    19561 acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct
    19621 ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca acatgattat
    19681 gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg
    19741 caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat
    19801 ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc acggacaggt
    19861 aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca acctgaatta
    19921 ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt tctgtgagct
    19981 tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag accgggctgt
    20041 gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc cgatcctgtc
    20101 cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg tggcgcaggc
    20161 ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca acgtggacat
    20221 cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca aaaagctggg
    20281 gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa
    20341 ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac agacggcggc
    20401 ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa caggtgccac
    20461 ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg aaaacagcga
    20521 cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa
    20581 tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg gccttttgat
    20641 ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc agctttacaa
    20701 gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg ccacaaagga
    20761 ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg
    20821 acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc agccgggagg
    20881 ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg ctggaacggg
    20941 ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc ggggctgtgg
    21001 ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg tcggaggaag
    21061 aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc
    21121 tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg
    21181 acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc
    21241 cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg
    21301 ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg
    21361 aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga
    21421 aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt
    21481 tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt
    21541 cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg
    21601 gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag
    21661 cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga
    21721 aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac
    21781 agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt
    21841 ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc
    21901 aagactatct ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg
    21961 acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga
    22021 tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc
    22081 tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg
    22141 cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac atctgctacg
    22201 tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc
    22261 taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca
    22321 gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg
    22381 ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc
    22441 cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt
    22501 gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca
    22561 tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa
    22621 cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc
    22681 attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt
    22741 acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt
    22801 gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag
    22861 cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg
    22921 cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag
    22981 gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac
    23041 cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt
    23101 tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac
    23161 aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga
    23221 cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga
    23281 cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca
    23341 gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg
    23401 cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc
    23461 caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc
    23521 ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc
    23581 taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga
    23641 actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat
    23701 tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa
    23761 cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt
    23821 cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac
    23881 ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg
    23941 cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc
    24001 ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt
    24061 cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc
    24121 caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga
    24181 tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca
    24241 ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc
    24301 attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat
    24361 ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag
    24421 cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc
    24481 gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac
    24541 gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc
    24601 gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag
    24661 gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg
    24721 tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg
    24781 cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta
    24841 aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc
    24901 gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag
    24961 gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg
    25021 gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct
    25081 aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta
    25141 aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc
    25201 gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac
    25261 ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt
    25321 atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag
    25381 ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag
    25441 gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc
    25501 ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc
    25561 gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca
    25621 gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat
    25681 acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc
    25741 aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc
    25801 tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg
    25861 gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc
    25921 gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg
    25981 cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc
    26041 gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa
    26101 aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt
    26161 gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa
    26221 gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt
    26281 ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca
    26341 gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc
    26401 ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca
    26461 gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca
    26521 gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct
    26581 gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa
    26641 cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc
    26701 cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat
    26761 cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat
    26821 catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg
    26881 acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa
    26941 agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt
    27001 tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca
    27061 gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga
    27121 accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat
    27181 cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct
    27241 ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat
    27301 gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc
    27361 gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga
    27421 gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaat
    27481 cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca
    27541 ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg
    27601 caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga
    27661 tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga
    27721 cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag
    27781 cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat
    27841 tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt
    27901 acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta
    27961 ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc
    28021 ccttgaaacc atccgggcca cggccagcga attgataacc aagatcgacg gccattttgc
    28081 ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac
    28141 cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc
    28201 agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca
    28261 aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga
    28321 ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag
    28381 ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag
    28441 ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg
    28501 catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc
    28561 ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca
    28621 gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt
    28681 gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact
    28741 ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca
    28801 ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc
    28861 ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg
    28921 gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga
    28981 ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt
    29041 caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt
    29101 gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt
    29161 gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga
    29221 aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga
    29281 acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga
    29341 ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg
    29401 cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg
    29461 gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga
    29521 gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat
    29581 gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa
    29641 tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt
    29701 tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc
    29761 cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca
    29821 ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc
    29881 ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta
    29941 tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga tacgccggtt
    30001 aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt
    30061 tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg
    30121 gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa
    30181 ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa
    30241 gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga
    30301 gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca
    30361 gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa
    30421 tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag
    30481 tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga
    30541 caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt
    30601 tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga
    30661 caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa
    30721 tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt
    30781 gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt
    30841 tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga
    30901 cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc
    30961 cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg
    31021 gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt
    31081 catggtggag atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct
    31141 ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt
    31201 gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc
    31261 tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag
    31321 aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa
    31381 cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt
    31441 catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga
    31501 acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac
    31561 agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc
    31621 tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc
    31681 tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt
    31741 gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg
    31801 ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct
    31861 ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat
    31921 atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag
    31981 ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc
    32041 aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc
    32101 cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg
    32161 cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa
    32221 acacccacat accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc
    32281 tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc
    32341 atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc
    32401 ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc
    32461 gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc
    32521 tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg
    32581 cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata
    32641 ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct
    32701 gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc
    32761 gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct
    32821 ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt
    32881 catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc
    32941 agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt
    33001 ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc
    33061 tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg
    33121 cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa
    33181 tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga
    33241 taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct
    33301 tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga
    33361 tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga
    33421 tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt
    33481 cccactcggc atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt
    33541 ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt
    33601 acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct
    33661 tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct
    33721 gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca
    33781 ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa
    33841 tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt
    33901 ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg
    33961 taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg
    34021 ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc
    34081 ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat
    34141 cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat
    34201 agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat
    34261 gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg
    34321 gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta
    34381 cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc
    34441 ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt
    34501 aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt
    34561 atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc
    34621 ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag
    34681 cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg
    34741 gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt
    34801 gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca
    34861 aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca
    34921 tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg
    34981 tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact
    35041 acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact
    35101 attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca
    35161 aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt
    35221 ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt
    35281 cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc
    35341 atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg
    35401 acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat
    35461 ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata
    35521 actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg
    35581 gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg
    35641 caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga
    35701 acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac
    35761 acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg
    35821 cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa
    35881 gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg
    35941 atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat
    36001 tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga
    36061 gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac
    36121 tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca
    36181 caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt
    36241 ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg gcttttctat
    36301 gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga ctggcttccg
    36361 tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc agcactgacg
    36421 tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga gatgaacagg
    36481 aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac gatgtggagc
    36541 ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat cttcaagatt
    36601 tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga taccgaacaa
    36661 cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg aaaggctaca
    36721 cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga acgagcgagc
    36781 atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca ggcaacgagg
    36841 tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc tacccaccag
    36901 acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat gttggcatcg
    36961 aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta aaaccagaaa
    37021 aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt tcccgttcct
    37081 gcttccaatg agaaaaagtg tgggcattac tgtttttgga tgcgaacagg atgaggcaaa
    37141 tgctttccgc gctttatcgc cggattttca tattatccct acgctgatta gcgatgcgat
    37201 atcggcagac aacgcaaaat tggccgctgg caatcaatgc gttagcgtag gccataagtc
    37261 cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat acatttctac
    37321 ccgcagcatc ggctgcgatc acattgatac gactgccgcc gagagaatgg gaatctcggt
    37381 tggcacggtt gcgtattcgc cggacagcgt tgcggattat gctttgatgc tgatgctgat
    37441 ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt tcagattgga
    37501 tcgtatccgg gggaaagaac tgggggatat gactgtggga gttattggaa ccggccatat
    37561 cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg cctatgataa
    37621 cagccgaaaa atggatgcag attatgtcca gcttgatgag cttctaaaaa acagcgatat
    37681 tgttacgctc catgtgccgc tttgtgcgga tacccgccat ctgatcggtc agaagcaaat
    37741 tggagagatg aagcaaggcg catttttaat caacaccggg cgcggagcgc ttgtcgatac
    37801 cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt
    37861 ggagggcgag gatcagtttg tttataccga ttgctcgcag aaagtgcttg accatccctt
    37921 tttgtcgcag cttttaagga tgccgaatgt gatcatcaca ccccatacgg cgtactacac
    37981 tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg aattgtctca attttgaaag
    38041 gagtttacag catgaataga ataaaagtcg caatcatctt cggcggttgc tcggaggaac
    38101 atgatgtgtc ggtaaaatcc gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg
    38161 atccgcacta catcggaatt acaaaaaacg gtgtatggaa gctatgcaag aagccatgta
    38221 cggaatggga agccgacagt ctccccgcca tactctcccc ggataggaaa acgcatgggc
    38281 tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct ttcccggttt
    38341 tgcatggcaa atgcggggag gatggtgcga tacaggggct gtttgtattg tctggtatcc
    38401 cctatgtggg ctgtgatatt caaagctccg cagtttgcat ggacaaatca ctggcctaca
    38461 ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca aatgattgat aaaggtgaca
    38521 agccggaggc gggtgcgctt acctaccctg tctttgtgaa gccggcacgg tcaggttcgt
    38581 cctttggcgt aaccaaagta aacggtacgg aagaacttaa cgctgcgata gaagcggcag
    38641 gacaatatga tggaaaaatc ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg
    38701 cggtcatggg gaacgaggat gatttgattg tcggcgaagt ggatcaaatc cggctgagcc
    38761 acggtatctt ccgcatccat caggaaaacg agccggaaaa aggctcagaa aatgcgatga
    38821 ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga
    38881 aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg
    38941 atggcggcat cgttctaaat gaggtcaata ccctgcccgg ttttacatcg tacagccgct
    39001 acccacgtat ggtggccgcc gcaggaatca cgcttcctgc actgattgac agcctgatta
    39061 cattggcgtt aaagaggtga taagtatgga aaatggtttt ttgtttttag atgaaatgtt
    39121 gcatggcgtc cgttgggatg ccaagtacgc cacatgggat aacttcacag gaaaaccggt
    39181 ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga
    39241 ggcacaaatc catgcggctg cgcttggcta tggcttgctt ttatgggatg gatatcggcc
    39301 aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca acctcacaaa
    39361 agaaaaattt taccccaata tagagcgagc cgagttgatt acaaagggtt atgtggcctc
    39421 acaatccagc catagccgtg gaagcgcaat tgatcttacg ctctaccacc tggatacagg
    39481 ggaacttgtt tcaatgggaa gtaacttcga ttttatggac gaacggtcgc atcatacagc
    39541 aaaagggata ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag
    39601 cagcggattt cagtcttatc gctttgaatg gtggcactat aagttgattg atgagccata
    39661 ccccgatacc tattttaatt ttgccgtttc ataatgaaag tattggtttt gtatcagatt
    39721 ttggatttga taaaatctat gctggtcatg gacaatcttt tactggaaag tggttaaagc
    39781 atcttttgta atacattgca aattatagta ttcaatctgc ccagcggcag aaaagaaaaa
    39841 aaccgctgtt gggcagacct ttcccacgcc cattttgtat tttgctggcg gcggttttga
    39901 gaaatcaagg ccgccgttct tttctggcag caaaaggtgt ggcggcccat aggaggatgc
    39961 cgctatgctg ttttgctatt gccatgctct aatccacttc caccactcaa aaaaagcctt
    40021 gcccctccac agggctattc cggttatgag tatgtactat accatgtaaa tcagcagcat
    40081 agcaaagcat tttctcgccc ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt
    40141 gcggcccggc tgtccccaaa aaaatttttt gagaaatttt caaaaaggag gtatttagcg
    40201 ggtagctggc ggccttgtca aagtcttgtt agcggaaagg gagagaacca cctaagtccc
    40261 ccacagaaag ggggtgagaa gatggatgct atcccccgcg actatgaagc gcggtgcatg
    40321 ttcgatgctt tttgcaagac ggtactgcgt aacgaggcca aaagctactt ggctgaaatg
    40381 aagcgccgcc gtgaccgtga agtgtcacta agctctttgt cacaggcaga tttggacaag
    40441 ctctgtacag tagatcacta ccccagcgac agctttacgt tttcgtcgca tggctatgac
    40501 ctacatatca acaatgagct tgttgccgaa gcctttgccg cgctgcccag catggagcag
    40561 agtattttaa tcctgcactg tgttctggat atggcagatg gtgagatcgg cggccttgtg
    40621 ggaatgtccc ggagtgccgt tcagcggcac aggacaaata ccttatcgga gctgcggaaa
    40681 cagctaaagg cgttgatgcc gaagggaggt tagacgcgga tgaagaaccc gaactacaag
    40741 ggcattgagc ttttgcccct ttcggtgatc gaggccgccc gcgctgggga tgccggagcc
    40801 gtagagcgcg ttttgcagta ttacagcggt tacatgaaca agctgtgtac ccggacgctc
    40861 tatgacgaaa acggctatcc gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa
    40921 atcaagctca tccacgctat cgtcacgaac tgacgaaccg gccccggcag catgaaacgg
    40981 cttacccttt ccccgtttcg gtgttttccc ggtggcctga tcgcaccttg ataaatatgc
    41041 ccgcaagata acggcggcgc atcatagggc atacggacac attctgtctg tgccgagccg
    41101 gacggctggt gcgccatgat acttttccct acgggaaaag cggagcgagg aacaccacgc
    41161 cgcaatgtga gcgtggcggc ttgcgggcga cgacgtacag gcaggactat gatactccct
    41221 tatagccaca gtccgagcgt taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc
    41281 acgcgggggt gaaagtcccg tggagctgtg ccagcagccg tccgtctatg cccctaaatc
    41341 acacaaacct ttgaatttgt gaaagggggc tgcattatga accatgatgt gcctatttgg
    41401 gaaaaataca cgctgacgat tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa
    41461 ttgcgtaagc tggccgagga aaatccggcc tctggctggg tcattatgaa tggcaaccgc
    41521 atccagatca aacgcaagca gtttgagaaa ataattgaca ctttggacac aatctaatag
    41581 tgaaaaaggg ccgcagatat gttataataa tttataagca tatatgcggc tctttccgtc
    41641 atggaaagga gcatgacgag atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc
    41701 ggtctggaga gagccagaga aaagacggac gatatgctta caaatatgtt gatacctttg
    41761 gaaaaccgca gtttgtttac tcatggaagt tagtgcctac ggataagacc ccggcaggta
    41821 aacgggagga tatttcactc cgggaaaagg agaaagaaat ccagaaagac cttgacgacg
    41881 ggatagacac catcggcaag aaaatgaccg tctgccagct ctacgccaag cagatcaggc
    41941 accggggcaa tgtgaagcac aacaccaaac tgggccggga acggctgatg cgtattctgg
    42001 aacaagatcg gctggggagc tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat
    42061 gggcgcttcg gatgaaagaa aagggccttt cttataagac catcaataac gacaagcgtt
    42121 ccctgaaagc ggctttttac accgccatac aggacgattg catccgaaaa aacccgtttg
    42181 acttccagct tagtgacgtt ctggacgacg atacggaacc aaaggtgcct cttacaccag
    42241 cacaggaaga aagttttctg tcctttattc aaggtgataa ggtttaccaa aagcattatg
    42301 acgcaatcgt tattctgctg gggacagggc ttcgcatttc ggaactgtgc ggactgacgg
    42361 acaaagattt ggattttgaa aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata
    42421 ccggcgtcgg ttactatatt gacgaaccca agacccaaag cggcgtcaga aaaatcccca
    42481 tgaatgagga agtgtatcag gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc
    42541 cgttcattat tgacgggtat gccaatttcc tgtttctcaa acagaacggc tatcccatga
    42601 cggcggtaga ttatggcggg atgtttggca ggcttgtgaa gaagtacaac aagagccatg
    42661 aagaagcgtt gccgaaaacg acgacgcccc atgctatgcg tcatacattc tgcacccggt
    42721 tagccaatgc gggaatgaac cccaaagcct tgcagtacat tatgggacat tccaatatca
    42781 ccatgacgct gaactattat gcacacgcca cattcgattc cgcaagggca gaaatggaac
    42841 ggctggcagc ctgacacaag gccggtttta ctactccatt tactactttt gagggggaaa
    42901 tggtgagagg taataagaac atttgtaagt atctaacaat atcaaaaatg ccggaaaagc
    42961 ccggaaacac gggctatacc gacattcaag aacttctaaa aagataatct aaaatcccat
    43021 ataattttag aataaaattt taaaaaggca aaaaattcgc cattttagga aagcgtttta
    43081 aaaaagtgtt gcaatctttt aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt
    43141 gtttaagtat aattaatagt gagaggaagt tgctctactg aagactttag ttatatggat
    43201 attgatgaca agcaacacta tgcaaagaaa gcggtcatca taatcgaaca acaagaaaga
    43261 gaaatcaaat gtaactggca ggagtgagaa tgtatggcat cgatccgtga cgtggctaag
    43321 atggctggtg tttcagtggg aacagtttcc cgttacttga atggtcagca actgaaggaa
    43381 aagaacatga aaaaaatagc agaagcgatt tcagcgttgg actataaaga aaacatcatt
    43441 gctaaagggt tgaagaataa tcgaagcttc tctattggac ttttgataaa cgggatttcc
    43501 agccgttttg ggtcggaagt tgtctcaggg atcgaaagag tagctgaaga aaatgggtat
    43561 agcttattat taagcgggtt tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac
    43621 ctgatgaagc atgtgatcga tgggctgatt gtcttcttat ccgaagaaga gtggtctgga
    43681 tttgaaatgc tcacaaaaat gagtattcca gttatcgcat tgaattgccc agaaggacca
    43741 gcaggtattg atacaatatt agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat
    43801 catggtgaac aaggacataa aaaaatcgga tttattgctg ccactcaaac agactacgta
    43861 gcaagagaac gtttagcagg agcaaaagaa gcggtacaaa atgatccatc gatccaatta
    43921 gaggtcttta caggagacta ttctagaaaa agtggctatt atggggcaaa agaattatta
    43981 gaaaaaggag taacagcgat ttttgtcagc aattataata tgtcgatcgg tgctatcgaa
    44041 tgtttcaatc aagaaggtat cagaatcggg aaggatattg cgtttagtca ttatgattat
    44101 ttggataaaa acgatttgag catccttccg aaaatcacga ttacaccacc aacagataaa
    44161 atcggggaac aagccgcaga acagctgttg aaacgaatca aaaaagaaga ggaagaaaat
    44221 gaaaaagtaa ttgtgatgga aaatatcata catggaatca actaaaacga actaaaaaag
    44281 aaaaagaggt gctttgatga aaaaatatca atttccagca aatttttggt ggggttctgc
    44341 agcaagtggc ccacaaacag aaggaaggat ttcaggtgat ggaaaaggag agaacatttt
    44401 tgattattgg tacaaaaaag aaccgcaaaa gttctttgac caagtaggac ctgaaaaaac
    44461 ttcacaagtt tatacgaagt atcaagagga tgtacagttg atgaaacaga ctgggcataa
    44521 ttcatttcga acatctatcc aatggagccg cttgatacct gatgaatctg gaacaatcaa
    44581 cgaagaagcc gttcgtttct atcatcgtta ttttgatgag ttgattgaaa acggtatcga
    44641 accctttgtg aatctgtatc atttcgatat gccgatgtat ctgcaagaaa aaggcggatg
    44701 gttgaaccga gaaacggttg aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt
    44761 cggttcgaaa gtaaaaaaat ggtttactca taatgaacca atcgttccag tcgaagcagg
    44821 ttatttatat cgatggcatt atccagaaga gtcagatatg aaaaaagcga tccaagttgg
    44881 ctatcatgaa gcgttggcct cagcgttagc catcaaatct taccatgaaa tggagcaagg
    44941 tggtgttatt ggtatcatct tgaatctgac accaagctat ccaagagacg aatcaaaccc
    45001 tgaagatgtt aaagcagcgc aaatagcaga tgcgttcttc aatcgttcat tccttgatcc
    45061 agctgtaaaa ggcgagtttc caagtgaatt gattgagata gtaaaagaac ttgatctcat
    45121 gcctgtgatc catccagagg atttgacggt catcaaagaa aatacagtag atcttttagg
    45181 tatcaattat taccagccac gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa
    45241 cggtccaatg cctgaggatt atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc
    45301 ttatcgcgga tgggagatct atgaacaagg aatctatgat attttgatca atgtacgaga
    45361 tcattatgga aatatcgact gctttatttc tgaaaacggt atgggtgtag aaaacgaagc
    45421 acgatttatc aaagaagatg gcatgatcca cgatgattat cggatcgaat ttgtccaatc
    45481 tcatctgaaa tatgtccatc aagcaattca agaaggtgcc aattgtttag gctatcatat
    45541 gtggacatgt atggataact ggtcatggac caatgcatat aagaatcgat atggctttat
    45601 ttcagtagac ttagcacaag atggaaaaag aacaatcaaa aaatcgggtc actggttcaa
    45661 agacgtatca gataaaaacg gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt
    45721 gaaattctcc attcttacca ttttgtgaaa aagaagttca attatttacg atctttttca
    45781 acttttttgt ctttctattt cattttttgt atataataga atagtcaaaa ttaaatagga
    45841 gtggaggaaa gcatgaggaa aaaaagaccg tttcttctct tttggttatt cggttttttc
    45901 ttactgctta ttttatccgc agcattttca ggaaaaatga cagcagatct tgctcagaaa
    45961 gaaacagaaa aaatcgtagc aagcaaagaa tcagtagata atttcaaaaa agaattctca
    46021 acttattggt gggcggagta taaaataact gccaattacg aaaaaggcga attggacatc
    46081 tatttgccaa aaggaaaaga agaaaatcaa tttattaata tcgatgacgt acttagtgcg
    46141 atttctctca gacaatatca atttaaagac aagcatttga ccgttttgct tttgactcat
    46201 gatagcgaca aagtagcccg tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa
    46261 gtagacaaaa aagtggtcgc cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc
    46321 aagaaaacaa ctgtctcttc tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct
    46381 ggcagtggac aaacgataac gggaagtgaa gaaaatcaag cctcaattat tacgaatgag
    46441 tcatcacaag gacaatatca atcctatgga caatgaaaaa
//
LOCUS       DACCQP010000017        42452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCQP010000017 DACCQP010000000
VERSION     DACCQP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.017"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(279..833)
                     /locus_tag="IVO87_001046"
     CDS             complement(279..833)
                     /locus_tag="IVO87_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7449259.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(959..2887)
                     /locus_tag="IVO87_001047"
     CDS             complement(959..2887)
                     /locus_tag="IVO87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7449260.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3193..3909)
                     /locus_tag="IVO87_001048"
     CDS             complement(3193..3909)
                     /locus_tag="IVO87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7449261.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3910..5733)
                     /gene="glpO"
                     /locus_tag="IVO87_001049"
     CDS             complement(3910..5733)
                     /gene="glpO"
                     /locus_tag="IVO87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7449262.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5747..7243)
                     /gene="glpK"
                     /locus_tag="IVO87_001050"
     CDS             complement(5747..7243)
                     /gene="glpK"
                     /locus_tag="IVO87_001050"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7449263.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7379..8839)
                     /locus_tag="IVO87_001051"
     CDS             complement(7379..8839)
                     /locus_tag="IVO87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7449264.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8829..10124)
                     /locus_tag="IVO87_001052"
     CDS             complement(8829..10124)
                     /locus_tag="IVO87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7449265.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10456..11133)
                     /locus_tag="IVO87_001053"
     CDS             complement(10456..11133)
                     /locus_tag="IVO87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7449266.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11163..12254)
                     /locus_tag="IVO87_001054"
     CDS             complement(11163..12254)
                     /locus_tag="IVO87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7449267.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12355..12789)
                     /locus_tag="IVO87_001055"
     CDS             complement(12355..12789)
                     /locus_tag="IVO87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7449268.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13373..14830)
                     /locus_tag="IVO87_001056"
     CDS             complement(13373..14830)
                     /locus_tag="IVO87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449269.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14827..16722)
                     /locus_tag="IVO87_001057"
     CDS             complement(14827..16722)
                     /locus_tag="IVO87_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449270.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16780..18555)
                     /locus_tag="IVO87_001058"
     CDS             complement(16780..18555)
                     /locus_tag="IVO87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7449271.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18605..20101)
                     /locus_tag="IVO87_001059"
     CDS             complement(18605..20101)
                     /locus_tag="IVO87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449272.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20295..21917)
                     /locus_tag="IVO87_001060"
     CDS             complement(20295..21917)
                     /locus_tag="IVO87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7449273.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22367..23680)
                     /locus_tag="IVO87_001061"
     CDS             complement(22367..23680)
                     /locus_tag="IVO87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7449274.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(23925..24149)
                     /locus_tag="IVO87_001062"
     CDS             complement(23925..24149)
                     /locus_tag="IVO87_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449275.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24169..24537)
                     /locus_tag="IVO87_001063"
     CDS             complement(24169..24537)
                     /locus_tag="IVO87_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7449276.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24733..25443)
                     /locus_tag="IVO87_001064"
     CDS             complement(24733..25443)
                     /locus_tag="IVO87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449277.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25461..25957)
                     /locus_tag="IVO87_001065"
                     /pseudo
     CDS             complement(25461..25957)
                     /locus_tag="IVO87_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(25994..26446)
                     /locus_tag="IVO87_001066"
     CDS             complement(25994..26446)
                     /locus_tag="IVO87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7449278.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26581..27186)
                     /locus_tag="IVO87_001067"
     CDS             complement(26581..27186)
                     /locus_tag="IVO87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7449279.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27636..28256)
                     /locus_tag="IVO87_001068"
     CDS             complement(27636..28256)
                     /locus_tag="IVO87_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7449280.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28258..29127)
                     /locus_tag="IVO87_001069"
     CDS             complement(28258..29127)
                     /locus_tag="IVO87_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7449281.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29150..29557)
                     /locus_tag="IVO87_001070"
     CDS             complement(29150..29557)
                     /locus_tag="IVO87_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7449282.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGINYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29576..30865)
                     /locus_tag="IVO87_001071"
     CDS             complement(29576..30865)
                     /locus_tag="IVO87_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7449283.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30892..31194)
                     /locus_tag="IVO87_001072"
     CDS             complement(30892..31194)
                     /locus_tag="IVO87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7449284.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31219..33312)
                     /locus_tag="IVO87_001073"
     CDS             complement(31219..33312)
                     /locus_tag="IVO87_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7449285.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33801..34529)
                     /locus_tag="IVO87_001074"
     CDS             complement(33801..34529)
                     /locus_tag="IVO87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7449286.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34675..37296)
                     /locus_tag="IVO87_001075"
                     /pseudo
     CDS             complement(34675..37296)
                     /locus_tag="IVO87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(37410..38741)
                     /locus_tag="IVO87_001076"
     CDS             complement(37410..38741)
                     /locus_tag="IVO87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449287.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(38707..40185)
                     /locus_tag="IVO87_001077"
     CDS             complement(38707..40185)
                     /locus_tag="IVO87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449288.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(40490..42325)
                     /gene="tdc"
                     /locus_tag="IVO87_001078"
     CDS             complement(40490..42325)
                     /gene="tdc"
                     /locus_tag="IVO87_001078"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7449289.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        12129 a         9198 c         7056 g        14069 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
      181 cttctcctgt ttttggtttg gttggcttat tatcgagaag tagctactgg gagtctattt
      241 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
      301 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
      361 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
      421 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
      481 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
      541 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
      601 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
      661 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
      721 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
      781 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
      841 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
      901 atttcactgt ttttattttt ctttacagcc actgatatta tgagctaatt tgagataatc
      961 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
     1021 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
     1081 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
     1141 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
     1201 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
     1261 gatcaccttt tgcaaagagc tggcgttctt ttttattctc cttcctattt gtaactgctg
     1321 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
     1381 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
     1441 ctcctgtttc tccaacccgc aactgatatt taggacgaag tgcttcccga tcgaaagcca
     1501 tagcagccgg cacaaaatct tcatgatctc cggcgaattt cttgatttct ccataatggg
     1561 tcgtggcaat gatcaatgcc ccttgctcat acatagattc catgattgcg atcgctagtg
     1621 cagccccttc atttggctct gttccactgc caatctcatc taaaagtacc aacgtattcc
     1681 tttttatctt tttcaaaata gcagcgatgt tttgcatatg accagaaaat gtactcaacg
     1741 cattttctaa attttggtgg tcaccgacat cgacgaaaat ctcatcaaag acagcaagct
     1801 ctgtcccttc ttttgccgga acttgcatac cgaacatcgc catcaaagtc aataaaccga
     1861 ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc ggtgataatc aagcctcgat
     1921 agtctttacc gatttcaaca tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc
     1981 cttgcttgat aatgatatgt tccgatttat tgattctagg tgtgcttcca ttgatttctc
     2041 ggctaaattt tgcccgagca aagatgatat ctaacaccgt gatcgtttcg atcaataaat
     2101 cgatcacttc ttcgttttct gcgatagcac cagtcaatgc cgccaggact tgatattctt
     2161 cactgatttc ttcagctttt aacaactgat agtgttcatt caatttagaa atcacagttg
     2221 gttcaataaa aaccgtcgtt cttttatttg attcatcgat aatcgttcca tctattttat
     2281 ttttatagct ggctttgata ggaatcgtat agtattcgcc tttttgaaca atcattgctt
     2341 cttggatcat ttctttgttt ttcggatgac gcaagaattt cagtaatttt gactggattt
     2401 ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac
     2461 tgactttatt attttttatt ttctggtcaa tcaactcttc tacatttatc aaatcaggaa
     2521 gatgtttgct ataagcaaac aacaagggtg tctggtattg attcttatca aaaaactttg
     2581 tgatcatccg actgcttcta agaaaatccg catattcgat caaatcggtt ggctgcagaa
     2641 tcaacccttt tttgacttgt tctgtcagtg catcgatcct cggcaacccc ataaatggaa
     2701 catgctgact gctttctaag atcaagcgtg cttctcttgt ttcttcttga cgatccaaga
     2761 cagtttgtag gtttgtcgag accgtcatat cgctgattcg ttttttgcta tagtttccaa
     2821 ttgctcttgc ttggatttcc tttttgatcc gatcaaattg taattgacta atcatttcgt
     2881 tgtccatatt ctctcctttt cttgaagaga tacctgtccc aaaataaata agggcctaga
     2941 gataaataaa aggatagaac acagcttaaa aaagtcatgt cctatcctta ttagaataca
     3001 aagatattgt tttcctgaca ggtaccgttt cataagcaca ccaaataaac tttgtttatt
     3061 ggctattcga cttactgatt agataccagc tacactcaca aaacataccc cattcttcta
     3121 ggtcttattt attttccact taactatagc aagacacatt ttggttgtca aactttagta
     3181 tgttttatag atttacacca ttaatgaata gacacctacc gcgataattg ctccgatcat
     3241 tggacctgcg attggtaccc atgaatatgc ccaatctgaa gttcctttat tagctattgg
     3301 caataccgca tgtgctaatc gaggaccaaa gtcccgagca gggttgattg catatcctgt
     3361 tggccctcct aatgaaagac cgatggctaa gatcaatcca cctacagcga atacgttcgt
     3421 tccaggtgca aattcgtttt gtccaaaagc tagcaaccca agaactaata caaatgtacc
     3481 aattaattct gtgatcacat ttgctggata attccgtata gcaggacctg ttgcaaacgt
     3541 tcctaaaatc gcgctttcat ctttcgttgc attccaatgt ggcaaataag aaagccaaac
     3601 aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt
     3661 ccaactaatt gcccctgtca tcgccatagc tagagagact gcaggattca aatgagctgg
     3721 gcccatgaat ccagaaatat agacagccat tgttacagcg agtccccaac caaatgcgat
     3781 gacaatccag ccagaagcct gtgctttact tttgtttaaa ttcacagccg cacacacacc
     3841 atctccaagc aaaactagga tcagtgtccc aataaactcc cccatgattt gagtcatatc
     3901 actgttcatt tatttctccc cctttagttc ccgcagatct gattctgcaa tcaatgcttc
     3961 taaacgttta gtctgttgcg ccttctcctc ttctgtccat tcaaaatatc cagcaattgc
     4021 atcgatcaca ggttgcttga tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt
     4081 acgacgaatg agataatcgc ctggggccaa aaccatttct tcttctaaac cataacgtaa
     4141 ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa
     4201 gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa gtagcatctt cttcctctaa
     4261 accggctgct actccttctt ttgctaattc tgtcactgtt tcttctaatt tagttggatc
     4321 gaagtttcca cctgaaatct gatatttttt agagtcgatt tctttcgtct ctattccgta
     4381 ttcttctttt aataattggc gtatcaatcg caaagctcct tctgccatct tacggtaatc
     4441 ggtgattttc ccaccagata aagtcagcaa accatctggt tctctttcta aagaactacc
     4501 tctggaaatc gtagaaggtg ctttttcatc acggctgttt tccaaatggt tcaacacgtc
     4561 ttctacttca gcacgagaaa ctttgttttc cttatattca cttactgtat cgacaacttt
     4621 attgaagctc ttgtctgaaa tcgatccatt atctccacca ttgtaatcag aaccagaatt
     4681 accaatcaac agtggacgaa gtcctgccca gcttgcttcg atatcattca atgtgatatt
     4741 tgcttctgga tagcgatgat tgatcacatc caatagataa tctacatctt cttgtgtgac
     4801 tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt
     4861 ttctcttgga atagcaaaaa ccatccgccc atcttgcttt cctgtatcga agtatgtggg
     4921 ttgcggtaca ggcagttttt tcgcatctac aactaaatgg atccctttag ttggacgcat
     4981 tttaggagag actggacgcg taaaattcaa gtttcgtact ttatctaccc aaggaccact
     5041 cgtattgatc actaattttg ctttgatttc gatcacttca tctgtcagca ggtcacgagc
     5101 tttgacgcca acgatttgat cgccatcata taaaaagcca accgctttca ttttacttac
     5161 tagataagcc ccatcttcgg cagccttttt gatattatca atcactaaac gggcatcatt
     5221 gttgcggaaa tctagataga cacccgcgcc ttttagccct tcttttttca aaaacggttc
     5281 tctttccaat acttcttctg gtgtcagcgt ataattctca tatttggttc ctgtcacatt
     5341 tgccagtttg tcataaaggt ccattgctac tttgaccgag aacatattga aggttgttgc
     5401 tccttcgccc tcatagattg gcaaaagcat tggatctggt tttgggatgt gtggggcaat
     5461 actttgtacg actgcacgtt caccaactgt gtcagctact acttccacat caaatgtctt
     5521 cagataacga ataccgccat gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa
     5581 atcctgcatt tcgatcaatc ctgttttgat tcctgatgct gctgactgga ctgccacacc
     5641 ggcaccagtg attccgccac cgatgatcaa cagatccaat tcttcttgct gcattttttg
     5701 aatgctatct tgtcttgtct tgtttgaaaa catatggatt tcctccttat tcaaatgctt
     5761 gggtagcttt tactgctttt ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca
     5821 tttgaacttc aaatagcttg ccagcttcat aaaattcgcg gatctcgtct gtatctttcc
     5881 aataaccgac agctaatcct gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt
     5941 ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa ttgcatcaga tattcattgt
     6001 ttgctgcacc gccatctact ttcaatacag gaatcttgat tccagtgtca tcttgcatcg
     6061 tatcgatgat atctctcact tgataagcaa tcgactgtaa agtagcttta atgatatctt
     6121 cttttgtcgt accacgtgtc aagccaaaca tagcccctcg tgctgcctga tcccaataag
     6181 gagcacctag cccaacgaat gcaggaacaa cataaacctc atcttcgctt gtagagcgtt
     6241 tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg aagtccatct ctcagccatt
     6301 gaatcgaaga accagccaca aaaatacttc cttctaaagc atagtaaacc tttccattta
     6361 tgccgtaacc aattgtcgtc aataaattat ttttggataa ttgcggtttt tcaccggtat
     6421 tcatgacaat aaatgaacct gtaccataag tattcttgac catgcctggt tcgaatgcca
     6481 tttgtccgaa taatgcggct tgctggtctc ctgccatccc tgaaattggt gcttcgcttc
     6541 cataaaaatg ataaccttgc gtataaccat aaacttctga gttagaagtg acttttggta
     6601 acatcactcg aggaatattt agtaaatcta aaatctcctg atcccaatct agctgatgga
     6661 tattgaacaa catcgtccga ctggcattcg agtaatcagt aacatgtgct tgtccatcgg
     6721 tcagtttcca tactaaccaa gaatcgatcg ttccaaacat caattcgcct ttttcagctc
     6781 gttcttgcgc gccttctaca tgatcaagaa tccagcgtat cttcgttgct gagaaatagg
     6841 catcgatcac taaccctgtt ttttcgtgga aaaattcttg atagccttca ttttttaagt
     6901 tatctgcaat tccagctgac tgtctggatt gccaaacaac tgcatgatag attggacgtc
     6961 ctgtatcttt ttcccatatt actgttgttt cacgctggtt cgttatgcca atcccagcaa
     7021 tctgatttgg tttgatccct gattcgatga acgctccggc aataactgac tgtacagaat
     7081 tccagatttc atttgcatca tgttccaccc aactttcttg cgggaaatat tgcgtgaact
     7141 ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa gataattgct cgtgagctag
     7201 ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt cattgtgatt ccctccatta
     7261 tatgcaaacg ctttctttaa ctactcttat catactactg ttttaaaaaa agaaactgtc
     7321 aaaattttat tctttttttc tttttttgac agttttttat tatgaagcta ttttttaatc
     7381 aattcttttc ttttttcagc gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg
     7441 acgcaaattc tgacagataa tattcacact gttttttgtt tctctggcaa aatgtgatca
     7501 ccaaatcata ttctttacct tcttcataac ttccaatctc aataccgccg attcccctca
     7561 gttctctgat cagatattgt tgaaaagcaa tacgataaat cggcagactt tctaaatcga
     7621 ttccaatcac tactggtttt gcaatataaa aatcgatcat caaaagaaca ttggcatact
     7681 ggatcatcag gtaactaagc tcactatctt cagctctttt cctcggcaat tgctgcaccg
     7741 ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg cccgagcatt ttctgctgct
     7801 gttccaaaag ccgatcacgc tcatagactt cgatctttcc aacaaagaaa aaccattcat
     7861 tgttgatctg ggaaatttgg tagcctactg ccttttcttc tttgattttc aaacgacgag
     7921 gacgataatg ccataacatc gtttcccgaa tgtaagtatc taaaacagcg gttggtaact
     7981 ttttgctccg tgtcaaatca tactgataaa atatttcttc gtctactatc gaaaaagaaa
     8041 caaaaaagct gtaaaacatc attgtttcgt atacattcag ttctgctgct gttcgactaa
     8101 gatgcatcac gatgattgga tcgattgctt gataaagccg gtccgactga tagagtaatt
     8161 tcttttcttt caaagttgcg tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc
     8221 cgagccagca agcaatctgt gcttctgctg atgccgtaaa cgttgtttct aatacacggt
     8281 ccaatcctag aatgaacggt cttttttctg gtgtattttg caaaaaaagc ggtcgctgat
     8341 cttccgggat atattggaat agttcataat aaaaatatcg tatctgtagc tcttctccgt
     8401 ataattgacc gtttttgatt tgtaatccaa attcctctaa gagctggttc aattctttga
     8461 tctttcgaaa aacagaagac tcactgatat taaacgctgt cgctaatcga acgatggata
     8521 cttgctgatg ttctaataaa tagacgagca tcttaaactt caagctttcc tgcaataaaa
     8581 acaatagaat tttttccaaa cttacatctt cgtccaacac aagcagcgcc tgatactcat
     8641 ttcgctgtac ttctactttt ttttccatca ttcggccaat caattcaata tcttccaaat
     8701 attgatcaaa cgaggctttt gacagttgga catgctctct caaaccattg atagaggctg
     8761 ttcctccttg cagaacaagg tttcgcaaaa tcaggatctg taactgctct tttctttcta
     8821 ataatgcttc aatcttcata ggcagtattc gccacctttg accacaccat atctggcaca
     8881 cggtattctg gctggaaaaa tagctcatgt tcttctaact gttcaaacgt ccatccttct
     8941 tttactgcaa ttgtcagttg gtcaagaagg tggaacatct cttttttgct ttggagctgt
     9001 gctccaagaa tctttttttc agaatcaaat agaaatttga gtttaacttt gctttgattt
     9061 attgctgtaa gcttgtagtc tacgtatcct gtttcaattt tttggggata aaaaatgctc
     9121 tcttcttctg tcaaacctac gcttcctaag aaatagccaa accactgatt gccgactgga
     9181 cgataggttc cctgatcctt tgtctgtgag ccactaattg tcttgctgac atttcgtgct
     9241 gtacggacag cgttatttac tagagaaaca tacatttttt catttgttgg acgaaaagtt
     9301 acttggatcg catcgccaat cgcataaacg tctttttgag acgtttgcaa gtagtcatct
     9361 acccagatcg ttccatcatc attcaatgtt aattgttctt cccacatctg attgtcgggg
     9421 cgagaatgat tagccagcaa aacaagatca tagttctcct ttcttttttc cgtctctaca
     9481 atagcggttt cattttcggt caaagacaca acttgttctt ctaaaaaaag tgaaagccct
     9541 tgatttttca tttcctttac taacggttct gtcatttcag gatctagata acgaaaaaga
     9601 attgactttc ggctttcgta aagatggatt tgtttttttc ttgatgccaa cccttcagct
     9661 atttcgattc ctgcttgtcc tgcaccaata attgcaattt tctttgcatt catgatcttt
     9721 tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt gaataagcgt tgattctgca
     9781 gaaatatttc gaaacttttg atttgaacca gtcgctagaa tcaaacgatc aaaatccaat
     9841 ttttttcctc ctgctaaaac gacttttttt tctttcaatc ctattactgt cttttctgtg
     9901 taaacatgga ttcgatagcg gtcagtcagt tcttctttcg tgatccaaga aagttgtttt
     9961 tcactggtaa cctttccttt taatatcaaa ttgaacccgc tgggaatcca tcctaacttc
    10021 gattgctttt caatcaaata tatttcactt gttggcatca attggcgaag ataaatagcc
    10081 gcatgaatcc ctgcaaacga cccgccgaca attacaatgt tcatctgcat tcctctttct
    10141 tataaggtgt tctactgcta acttctcaat cagaaaacac agtattcata actcgctttc
    10201 aaccttatga tacatagcga tgcagcagaa tcaaccctct ccatcatacc gtgttctagg
    10261 tctgcttctc aataatgtac tagtggacct aattgaagag tatcatttct gaagagatgt
    10321 ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat
    10381 aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg taatgtaata gttagaatag
    10441 acacatcatt tatttttaat tctttaatgc ttcaacaact gcttcgccta attgatgagc
    10501 agattgagga ttttgccctg taagaagccg cccatcaatt tgaaccactt ctgaaaaagc
    10561 tgctccagac tgatagtttg ctcctttttc tttcaaagca tcttctgcta agaatggtac
    10621 ttcattcgtt gtaccatttg cttcctcttc ttcattactg aacccagtca ctgtacgtcc
    10681 agctaaaata gattttccag actcatctgt aactggcaac aatcctacaa caccgtgaca
    10741 aacagctgag accagccctt catttcgata gatttgtgaa gcgactttcc caagatctat
    10801 tgcttctgga aaatcccaca ttgttccgtg gccaccagca taataaaccg ctgcataatc
    10861 atttgcatcc acatcatcag gacgtaaggt atttcccaag gcccgatttc tgaaatcttc
    10921 atcttcataa taggcccaat cgatttcatc catattcaaa acactttgcg gttctaaagg
    10981 tacatatccg cctttaggac tgacaaaatc tgcatcgatc ccatttttta ctaatacatc
    11041 ataaaaatga gtcagttcac ttaaccacaa acctgtagga cgctcgatct tttcgtactt
    11101 ttctacattt gttaatacga tcaatacttt cattttccca tcctctttct ataaaaattg
    11161 cgttattgat aacgtgcttt caagaaatta tataatgggc ttggcaaata aagctcttta
    11221 tatgcttctt caaaaggttt cgttacaaat tcatcctcac ggttttccaa taatttctct
    11281 gcccagtcag gatcgacgat catttgctgt ccaattgctg caagttctgc atatttcaat
    11341 acctgctcta catcttcgcg attgcggatc ccaccgacac caactaaagg gatctttcct
    11401 tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt tgtatttatc agaacgagct
    11461 attcgatcat acatatttaa tgaaacatgc agataatcta gttttgactc acgtaatcgt
    11521 tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg tttcatattc ttctggtgag
    11581 aaacgatacc ctacgatgaa tggtttttta gcatattgtt caaccgctga gaaaaccgtt
    11641 gttaataatt cttcaataaa acgataacga ttttccaagc taccgcccca ttgatcctct
    11701 cgtctattcg aatgcggaga gaaaaattgt tggatcaaat acgtatttgc tccatgcaac
    11761 tcgattccat caaatcctga ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt
    11821 gtttcaacga tttcttcttc actcattgct cgtggtgttt ccgcatcagg acgttttgct
    11881 gcaactgcac ttgcagatac aggctgttct ccagataaag ttgctcgact tgtcattcgt
    11941 ccaccgtgga aaatctgaat aatcgctttt gcgccttctc cttggattgc tttcgctagc
    12001 tcacttaatc gaggaatcat ttcatcatga gcgatgctga gttcacctgg ccagcctttt
    12061 ccgatatctt ggacatttgc agcaccagta ataacagcac caagaccttt agaacgttgt
    12121 ttataataag tgatctcatc ttcggtgatc actccattga aaaagctgag ttgtgtcgtc
    12181 ataggcgaca gcatcaatcg gttcttcaat tgaactcctg aaggtaaagt aattgattga
    12241 tcaaattgtg acattgtcat tcctcccata attcgctctg attaacctat cgagaatcat
    12301 ctatcattaa accactcgat tagttgtaca tgcaactaat ttgcttaaaa aattttattt
    12361 tttttgtaac ttatctgttg catcattcat tgtctgggct aattctttac ttgcttcatt
    12421 tcccaaaact gctttgtatg actgatgaag ttcttcccag ctaccatcga cttccttagc
    12481 aaattggcgt ccttgttctg taagataaac taatgacaag cgaccttcgc taactgtttt
    12541 gacgtatcct ttatattcta atttttcaac aaaacgagca atcgttgatg gggcggaata
    12601 gagcatttgt gcaatatctt tttgtggtat tccatctttt tcttttaata tcaataaaat
    12661 aaaaccataa gttggtgcca gacctgtcct tctaaacgct tcatcagcta atttattcaa
    12721 catgcgatct agctttttca cgctaaagaa aagacattca tctaacgctt tttcaattgg
    12781 cttttccatt tcatcccctc cattggcgta catatcatag tatgattttt attttttgtc
    12841 aaaaaacacg gaataatgtc cgcctcctct gtctagattt gaaaaaaaga agtagccatc
    12901 ttcaaagaat ttcggcttgt tttcgacaaa actccttctg gaacaccgtg aatcagaggt
    12961 tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt tgtgaaaatg gctttccaca
    13021 tttttgcata aatttggctt aataccgaaa aactggcttt tgaacaccgt ttatatcaga
    13081 ggttgtgaaa aagccgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    13141 atattttgtc gttttttgag ctaatgccga tggtcagctg attgtacacc gtttattgag
    13201 aggtcgtgag cctgccgttg aactccgagt cacaaggagc aatttcaaaa aacagcttct
    13261 catgttttat gactatggtg acttgtgatc gaggagttgg caggagaata ctgtttattc
    13321 gtaaagaagg ggctgtgaca agacttttgt cacagtccct tatttccttt tattatagga
    13381 tttcgccatt tgattggatg acttgtctgt accaatcaaa tgattttttc tttaaccgtt
    13441 tctttgtgcc attcccttcg tcatcttgat caacatagat atatccataa cgttttgaca
    13501 tttgtgaagt agaagcgctg acgatatcaa tgcagcccca cgacgtataa ccaaataaat
    13561 ctactccatc ttcgatagct tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc
    13621 ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc tatagctccg agtccgtttt
    13681 cagctatgaa cagtggtttt tgatacctat catataattg attcaaagca attctcaaac
    13741 ccttaggatc aatctgccag ccccattcac ttgatggaag atacggattt ttaattcctc
    13801 cagttattaa gtttccttgg accccttcca aattttctgg atgagcactg actgcactag
    13861 acatgtagta gctaaaagaa acaaaatcaa cagggtaggc tgctaagatt tcttcatcac
    13921 ctacttctat ttttaagttg acaccttttt cttcaaacat tcgttgcgta tacgctggat
    13981 aatatcctct tgcttgaacg tcactaaaga acaaagaagt tctttgctgc tcaattgttt
    14041 tttgaatatc atccggatca caagaatacg gaacaagaag ctgatagctg atcatacaac
    14101 cgacttgtga acctttgata atttcatgac aagcttttgt agctaaagca cttgctataa
    14161 actgatggtg tgcaccttgg tatttcgctt gttccaaatg gggatggttt tcttcaatta
    14221 ctcctacgct aacaaaaggg tgatgtttta cacagttgat ctcattgaaa gtcatccaat
    14281 attttacttt atttttatag cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa
    14341 taagctttcg attaggccat ccaccatatt ctgtaaccaa atataacggc atttcataat
    14401 gggaaagaga aacgactggc tcaatcccat attttagaca ttcgtcaaac acttgatcat
    14461 aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc
    14521 atgcgataga catccgatag catttaaaac ccatttcagc aaataaacgg atgtcttctt
    14581 taaagtgatg atagaaatca tttccgtgtc ttttaggata ataatacttg ctgttgggat
    14641 cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca ttgatctgtc catttttctt
    14701 taggtaaatc gctgtcgaat gtatacacat cagacacaga taatcctttt tcatctacat
    14761 cccatgcacc ttcacattga ttggccgctg tcgctccgcc ccataaaaat ccttctggaa
    14821 attttttcat atcgatcccc ctaaacttgc tagttttctt gtttgattat ttttcttatg
    14881 actagtaatg atatttttga atctactact atttgtaaca ataaccattg tggtcggatc
    14941 atatccctct ttgattaatt cttgcaaatc tacttccatc aataaatcgc ctttacgtac
    15001 tttttttcca tcagatactg cactttgaaa gtatttgcca tttaaattca ctgtatcaat
    15061 accgatatgg atcagtatct ctgctccttc ttcactgact accccaattg catgtttcgt
    15121 tggaaaaact gttgaaactt ccccgtttac aggactgtag atttttccgt ccgttggaat
    15181 gatcgcaaac ccttctccta aagatccact agcaaatgtt ggatcgttta cttcagataa
    15241 atcaattatt tcacctgctg caggagtact taacactata tcctttgagg attcttcttc
    15301 gatttcatcc ttgtagccga aaagaaaagt tagcatacag gaactgatca tcgctaagaa
    15361 ataactagca actagccaaa caaatccttc accaatataa atcgggaaag acaaaatact
    15421 tcttgtagca actgccaaag caccggctcc tgcggcacca gctactccac caaatatagc
    15481 tccacatata caagcaatgt aaaatggtcg tttatattta agagtcacac cataaacagc
    15541 aggttcagtg attccaaata atgctgtgat aaatgcagat aaagataaac tttttacttt
    15601 cttatttttc gttttcaaaa ataccccaag tactgctcct gcttgcgcag ctacggcagt
    15661 catacatgct ggacctagcg tatcacgacc gtagaaagaa agattgttgg tcattactgg
    15721 tacaagtccc caatgtaacc caaaaatcac taacacttgc caaaatccgc cgataacagc
    15781 accagataaa acagggctaa agttataaac agctaaatat ccagctgcaa tcagctgact
    15841 tgcataatct gcaattggac ctacaactaa taaagtaatt gggaccatca cgatgataca
    15901 aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc
    15961 aatataagaa agaacccata cagctaaaat tgctggaata accgtacttg tatatcttgc
    16021 tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta tcactatatg cactgacaat
    16081 cgtaggataa acgagagctg ctgcaataac catagcgatg aaacgatctg tattgaactt
    16141 ttttgcagca gtatatgcca aaaatatagg caagaaatag aaaaatgcat cagctgctgc
    16201 attcaaaatg atataagttc ctgattcagt agttaataaa ttcaaactcg tacataaggc
    16261 gagtactcct ttgatcaaac cacttcctgc caagacgcct aaagtcgggg caaaaacagc
    16321 ggcgatcatg tctataaaag agtttaaaat gccttttttc tcagttcctc tctttccact
    16381 agatgcatca tcttctagtt tcatttgttt ggataatacg tcatacactt catttactgc
    16441 atttcctact acgacttgaa acattcctcc agcttcgact acagtgatca caccttctag
    16501 ttgctctacc ttctccttat ctgccttctt attatctttt agggtaaagc gtaaccttgt
    16561 tgcacaatgg taaacattgc tgacattttc ttcgcctccg acaaatcgga caatatcttt
    16621 cgctaattgt gaatagtcca tttttacttc ccccttgtta attaatgccg tagcaatcct
    16681 tcttacaaga taagtatagt tcttagaaaa cggtttctcc attcaattag taccccttct
    16741 aatagcgaac gaaactgtac aatagtgtgt atggtttact tagccgagca aatcactcac
    16801 tgcttgaaag aaaacaagta tatcgtttct cttatagtca aaggagacaa aaaaaacacg
    16861 cttgatcttc ctttgctgat gtataagtgg tatctcaagc tgatactctg tgatttttgt
    16921 ttctacttgg cttttgataa gacagcacaa caaagtctcg gcatttaacg gaagcataac
    16981 taggtcagaa ctaggagaga ttagttgctg aatggaatct atctttttat ggatttttat
    17041 ttgattttct tctttgtagt aatgatcaag cagttttgtt ttcttttttt cttcaactat
    17101 ggtcagcttt tcacgcaaaa tctgataaat ctccgaagat tcaaactcat ctagaacact
    17161 aggtagataa atcaaatcat cccactcaaa aataagctct tgctcagtag tcaaaattgc
    17221 atcatattcc gttattttgt ttgattgatt tttgaaaaga tacactggtt ctatccggca
    17281 ttctttgatc cacatgttca tactttgttg aatctgtttt tttagtgtat tcaaaatact
    17341 aattggctgg ttactaacaa tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc
    17401 ggcaagatac aaaaccaaat aagcgctttc agctaaattg ggacgtaatt gaaaatgtat
    17461 tgggaagaaa cgccgaatta aataaatttc taaaggataa cgggagatga tcttttgtac
    17521 aaaatgattc atggtattat gtccggcatc catcctcgtt ttcatttgtt tgatatgggt
    17581 tagcaaaaat tctgtttcat ggaacaataa cgaaacagga agatctaaaa aacgaataat
    17641 cgtttgctca aatgctctta gattcttctc tatttctttg tttttttcat cattaatcat
    17701 caaataattt aactctaata gtcgattttc aattgcttgt ttttcttgat tggaaaaaga
    17761 atagggtagt tctgatgata acttttttgt gagggtctct accatcggaa taaataatgt
    17821 atacctgttt gttttttcta aaagttcttc tcttcgaaga atggtcaaaa cgagataccg
    17881 agaaaatcgt atgaagtcct ctcctgaaac aatataatta tatgatataa gaacctcttt
    17941 taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa
    18001 ccttgcgtgt tggatcaccg tttctgttcc aacaagagat aagaaaattc tttttgaatt
    18061 ttcttcagca atgattttta gtccttttgt cgacgaaact tccagttcta atttaggatt
    18121 gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa
    18181 gaaaatgtct gctatctttt gcatactgac ataccctttt tgaaacaata agtacgtcac
    18241 aatagttgaa gctcggaaat ccatgctgga atagatttta tgtttcgtca cctcttgtat
    18301 gatttgctgt ttagtttcag attcaattga ttctaaaaaa taacctgaac gtttatttga
    18361 ctggataatg attttcttct cgtagatagc aagcatattt ttgatttcag tttggatcgt
    18421 tttcttgttg gtacctaaag cttcagctaa ttcttcgctt gttatccaat gattcttatt
    18481 tcttaatact acaagaattt ggagttgtcg tttatttaag tccatttgct tacacctcct
    18541 ctttgttata aacatacagg aaaaacctct caaatgacaa tagtcacttg cagaggttta
    18601 aaaatcattt gaagaaagat ctatattcaa ggtttttacc attagactgg ataatttgtt
    18661 tataatattc aaaactatct tttttcaatc tcctaaagct tccgctccca tcattttgtc
    18721 tatccacata aataaagcca tatcgcttcc tcatttcccc tgttcctgcc gatacgatat
    18781 cgattggtcc ccaccataag taaccaatga tttggcaacc atcttttatt gcctcagcaa
    18841 tttcctgaag atgtattttt agatactcac aacgttttgg atcgtggatt cgattcaatt
    18901 catctggtgc ttcatctagt ccaatgccat tttcaactat gaataatggt aattcataac
    18961 ggtctgtcaa ttcattcatg acataacgaa accctagagg atcaattggc caactccaag
    19021 gttcgggaga tttttccttc aaaaagggat tatcgaaacc atttgctccg ccagtatcta
    19081 cgcgcatttc tgcttctttt ttatagacag cgctacgata ataactaaat gcaatataat
    19141 ctaccgtgtt agcttgcaag tactgttcgt ctccatctaa cattactggt gcacagtctt
    19201 cttccttcca tatcctgtaa atataagctg gataatgccc tcggcacatg acatcggaga
    19261 aaaaccatga attgcgttta aattgcagtg aaccaaatac attctctgga tcacagtcat
    19321 atgcataagt cgctaatgca gaaaagctac acataatacc catcttagca tttggatcga
    19381 ttttctttaa taattgagta gcacgggcgt tcgctagaaa catatgatga caggcttgat
    19441 aaatctcttt ttttgttagt gtagcaatgg gttcaagttt atctctagca gggaatgtaa
    19501 ccaatccagc agctgcataa ggcatacgaa aagcattatt gatctcatta aaggtcatcc
    19561 aatatttcac tttcccttgg tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt
    19621 cgattaattt tcgattttgc caccccccat actctcctac caatgctaag ggggtttcat
    19681 aatgactaag agtaattact ggctcgatat catatcgatt taatgtattg attagtctgt
    19741 cataaaatac gagaccagcc tcatttggct tctgttcgtc tcctcttgga aatattcgtg
    19801 cccatgagat gcttgttcga aaagctttta aacctaactc cttcagtaaa cgaatatcct
    19861 cttcaaatcg atgataaaaa tcaatcgctt catgagaaag ataatggagt gattcgtcta
    19921 aagcaatctc ccaaatcttt ttttcttcat tccactgaat actaggatgc tggttcatga
    19981 tcccgataag cgtatctgtc acattaggta atttgccgtc ttccaaccat gcaccttcac
    20041 attgattagc agctactgct ccgccccata aaaaattttc tggaaatatt tcctgatcca
    20101 taactatctc tcctcgctat caacaatttc ttacttgaag tatagctcga ttcatcaaaa
    20161 caagatattc taactatttc ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt
    20221 ttaatattta tatataacaa atacataaga cgatccccga aagaaaacac tcgttaacca
    20281 tctaagcaaa cgatttattt tacttccaac acaaatgctt cataaggtcg taaatcaact
    20341 ttgccgtctg gtaactgttt cagaggataa tttgacacaa taacccttaa agcttcacgt
    20401 ggaacagtga acgtctgctt ttcactggac atattacaaa taacgagcca ttgctggtct
    20461 tcatatatgc gagaataggc aaagacatgc tcaggcgtct tttccactaa ttcatagtct
    20521 ccccagacga tgatgtcatg ttttctacgc aaatcgatca atttcttgta catataaaaa
    20581 acagaatctt gatctgctaa cgcttcttct gcattgattt ctttataatt tggattgact
    20641 cgaagccatg gaattccagt agtaaaccca gcatgttcac gggcgttcca ttgcataggc
    20701 gtcctagcat tgtctcttcc tttcgcattg attgattcca agatttcctc ttttgtaaat
    20761 ccagaagaaa tcctttcgtt atacatattg attgtctcaa tatcttttgc ttccgcgata
    20821 tcatcgattg ggcaattcgt catgcctaac tcttctcctt gatagatata aggggtgcct
    20881 ttcatcatat ggagcaagat agcaaacatc ttggcacttt cttttcgata cacatcgtca
    20941 tttccccaac gagaaacaat tcgcggaaga tcgtgattat tccaaaataa actattccat
    21001 ccttcatcac ctaaagatgt ttgccatttg gataatactt ttttcagttc agagatactc
    21061 aagggtttca aatcccattt gtctttgcct tcttgctgat ctaatcctat gtgttcgaat
    21121 tggaagacca tggataattc atttctttta ggattggaat aaagtttagc gatttcggta
    21181 gttgctcccc atgtttcgcc aacagtcatc agatttttgt caccaaaagt tgcttgattc
    21241 atttcctgaa gatactcatg cagtttaggt ccattacttg tgatttcttt atccggttct
    21301 ttgccaatca tatcgatcac gtccatacga aagccaccaa tacctttatc gatccagaaa
    21361 ttcatcattt gataaatctc ttggcgaacc tgtttatttt tccaattcaa atcaggttga
    21421 cgcttgctat acaagtgcag aaaatattgc cccgtttttt catccaactg ccaagcagaa
    21481 ccactgaata ttgagcgcaa tctattaggc tcttttccat tgaccggatc acgccaaata
    21541 taatagtccc gataaggatt ttctttattt ttacgggcct caatgaacca cggatgttca
    21601 tcagaagtat gattcaccac cagatccatg atgatacgga tatttctttt ctttgcttct
    21661 tcaatcagtt cttccatttc ttccatcgta ccgaattctg ccatgattgc ttgataatta
    21721 ctgatatcat acccattgtc atcattcgga gactcgtata ctggagaaag ccaaattgca
    21781 tcaataccta aatacgccag ataatccaac ctggaaataa tcccccgcaa atctccaata
    21841 ccatcttgat ttgaatcttg aaaacttctt ggatagattt gataaacaac tgaatggtgc
    21901 caccattttt gttccatgat tttcctccta taaatggatg tttctatttt ttgcttaatc
    21961 atttatttta gtctctctca ctaaaaattg taacgctttc aaatagctat tgcaccttgt
    22021 tatcggtaac agaataaaac aaaaaaacag ctgaggaaat tccctcaact gttttttgaa
    22081 aggattgatt tattcaggga agaatgctga tttcacaact tctttataaa cggtgttcac
    22141 tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta tttttttcgt
    22201 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    22261 actcagctgt cccttggtat tagctcaaaa aaaggcaaaa tatgaggaac tattttttcg
    22321 tttttttctc taatactcag gccagaacac tgatttcaca acctctttat ttcacattca
    22381 atatccacat gaatcccatg aatacaaaga acagaccata catgatggga gatacttctt
    22441 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    22501 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    22561 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    22621 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    22681 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    22741 cagctgatga ttctacaaat gcaccaacag gagaagtacc taataaggaa cccgcaagca
    22801 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    22861 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    22921 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    22981 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    23041 tcaaacctgt gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg
    23101 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    23161 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    23221 cttcgctcag accaaggaaa gcaataaata gaccgatacc gccagaaatc gcatatttca
    23281 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    23341 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    23401 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    23461 aacgggctaa tacccccatt aaaatacagc cgatcgcact ggctaatgca gtcgcagtaa
    23521 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    23581 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    23641 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    23701 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    23761 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    23821 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    23881 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    23941 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    24001 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    24061 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
    24121 taaccttcta atcttcccat tagatccact gtttctcctc ctttttattc aatcagacga
    24181 ctcagctttt cctataacaa aataattgcc ttcagggtcc gaaaaattga aagtcaagac
    24241 gccattgttg tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
    24301 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
    24361 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
    24421 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
    24481 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcgt
    24541 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
    24601 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
    24661 ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt
    24721 atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact
    24781 agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg
    24841 tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt
    24901 cagcatcgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc
    24961 atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa
    25021 tgattcgtca ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc
    25081 tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt
    25141 gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg
    25201 tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt
    25261 ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa
    25321 ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa
    25381 gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt
    25441 catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga
    25501 tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa
    25561 gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct
    25621 tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt
    25681 tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc
    25741 gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa
    25801 tctgtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag
    25861 taatcagcta aatgctccgg taattatctt ttccaaatgt atctgtaaat gtttcgatac
    25921 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    25981 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    26041 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    26101 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    26161 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    26221 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    26281 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    26341 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagagt
    26401 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    26461 attttgtttg taagactagt ttacctcttt ttgttgcaaa tgcaactatt tttaataaaa
    26521 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    26581 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    26641 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    26701 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    26761 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    26821 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    26881 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    26941 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    27001 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    27061 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    27121 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    27181 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    27241 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    27301 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    27361 tgcatctgcg gtaataagcc ccatcaaacg aaaaatatga cgagctattc ttgtctggct
    27421 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    27481 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttctttgaa
    27541 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacggtgttc
    27601 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    27661 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    27721 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    27781 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    27841 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    27901 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    27961 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    28021 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    28081 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    28141 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    28201 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    28261 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    28321 aatttctttt actgctttat ttgctggaat cacattatgg cgaagagact tgttgatttc
    28381 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    28441 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    28501 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    28561 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    28621 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    28681 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    28741 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcaaga tattttcttt
    28801 gaaaggtaga tgggacccgt catacatgac agaagtatat ccgctatcga ttgcttcttt
    28861 gatatcatca aagtctcggg cgtggtctaa gtgtaagact gcatcaacca tttcttcttc
    28921 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    28981 acttgtttga ataatgattg gcgaacccat ttcttttgct gcgcctagca tggcaggaag
    29041 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    29101 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    29161 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    29221 tgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    29281 tcagtttttt atacccatct ccgatcgtct gtttattttc caagctcttt tctaaatatt
    29341 ggatggcttg atcgttctca tgcaattcag aaaatttaag tccaacttgt tcataaagtt
    29401 ccgctaattt atgcctatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    29461 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    29521 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    29581 acgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    29641 catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact agcaaagcgg
    29701 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    29761 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    29821 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    29881 ttgtcgtaat gaaacctaaa atgacggcat ttgggctata aggaaagaaa actggacaat
    29941 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    30001 tttcacctaa cagtagacgt acaccagcta gcaatatata cacacccact acgaattgga
    30061 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    30121 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    30181 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    30241 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    30301 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    30361 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    30421 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    30481 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    30541 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    30601 cttgatcatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    30661 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaatactt
    30721 gaaatcctag gagtgttttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    30781 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    30841 cgatgataaa attgataacg ctcatgtttt taccctcttt tctttttgta tttagataac
    30901 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    30961 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    31021 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    31081 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    31141 aaaactactt cctaaacctg cgccgcatac gaataaaatg ttcaatttgt tcatctttgt
    31201 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    31261 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    31321 ttctttcatg atgttcagat gggagtagga gtctactgct gccaaacaaa aaataatctt
    31381 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    31441 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    31501 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    31561 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    31621 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    31681 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    31741 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    31801 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    31861 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    31921 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    31981 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    32041 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    32101 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    32161 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    32221 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    32281 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    32341 taaggcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    32401 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    32461 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    32521 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    32581 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    32641 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    32701 gatctcctgt atttttttga tttcctccaa aggaaaataa tggagaaaga ttttttgagc
    32761 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    32821 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    32881 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    32941 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    33001 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    33061 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    33121 caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    33181 gatattcgct tcaagaactt cctgacgaat cgtccgctga ctgacattga actcacttgc
    33241 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    33301 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    33361 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    33421 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    33481 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    33541 aactaaacta aaatagtgct tcctattttt tgtttggttg ttcttattat cgcagatgca
    33601 agcttttaaa caccatttag tggagaagct gtgacagaga tgttcagcct tgagcgataa
    33661 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    33721 ctatctctgg aacaccgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    33781 ccgttcataa atatttcctg tcaatccagc ataatcggtt gaataaaatc ggcttgccat
    33841 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    33901 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    33961 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    34021 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    34081 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    34141 caagcggatt ggatcttcat gaatatcttt tggatttcca aaaactgtgc tttcttttct
    34201 ttagccgttc ctgacatgcg ggacaatatc tccgatttca gccttttctt ttgtctttta
    34261 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaac
    34321 cccgtttgct cataactaga aggacctgta cttggaccat aatacccata agaatctctg
    34381 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccgccaaa tgaacctctg
    34441 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    34501 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    34561 tcattgtctt cctcgttatc ttttattaca aaaaactatt tttatcatag ccttgctaaa
    34621 aggaagagtc aataaccaga tgcataaact gtttttttag caattttgtc ttatttagtc
    34681 attgagtaac atcggacaga tgtatgtgtc ggcagtccat aattcctcta tcttcagcat
    34741 gaacaagctt gcagacaaga gatctgcgct tcctccagga gagatttggt tttcctgaca
    34801 aaagtgcaac agtgctgtaa atggacgatt tggtaaagca ctttgactag cagcatgagc
    34861 aagtttttgc atttccacta acgttttctg atcactgcga tgcaatacgc atgtgtcatc
    34921 tagcgaactc atcaaaacta acaacatatg catttgtttc tgtacatatg aatcattatc
    34981 tagctcctta gctgcttgga ttgcaagcct gatatgcgga taccctaata tcgcttcttc
    35041 atatgcacca tttacgccat atttttgctt caccctatga ccatgagtgg tttttttatg
    35101 acgatatttt gtatcaggaa aagcagcaag cttgctgaca tcatcaagta ttttcgataa
    35161 agaaaaaggc tgttccatac tgattttttg tgcaatgaca ctgatcaata gccccattgc
    35221 ccaaatcgct cctttatggg tattgatgcc atttgtagca atcagcattg ccttttccgc
    35281 ttttcgccca atctcagcaa ttctttctct taactcctga tcaatagcat aatgccatgc
    35341 tatttgagcc atttcttcaa aaaaaggagt tagcgtctta gcagacttat ccaaaatgcg
    35401 ccgaccttct tttacggctt ttgcgatcgt atggaaatta tcatctccta aaaccatatc
    35461 agctgcctgt ttgcttactt cactaccttt gattcccata gcgattccta tatcggctct
    35521 tttcaaagca ggtgcatcat ttacgccgtc tccggtcatt ccgacgattt ctccattttt
    35581 ctgcaaagct ttcacgattc gcagtttgtg ttctggtgtc gttcgagcaa agacgtcgac
    35641 ttttggtact tgtcgaatca actcttcttc agacataaga tccaaatcga tcccttctaa
    35701 aactttagca gtatgtttta agccaatttg ttccgcgatc gcttttgctg tatctttgtg
    35761 atcccccgta atcattttca ctgagattcc tgcttgttgg cattccttta cagctttgac
    35821 tgcactttct tttggcggat caatcaaccc agcaatccct gcaaaagtta attgggttaa
    35881 agactcatgc gtgagttcct gtttcgaatc tacttttttg taagcaaatc ctaaaacacg
    35941 ctgacctttt ttcgcaaacg ctgtggctgt ttgaccccat gcttcttatt ctgaagaaga
    36001 aagagttgat ttagccaata atacttctgg cgcacctttg acgtaaatca cccctccttt
    36061 ttcctccgca tgcatcgttg ccatatattt gtattcagag ctgaacggaa tttttgcttc
    36121 tatttttttc aaaggcagat cggctgtttc ggtgtggcga atcagtgcaa gttctgtcgg
    36181 attgccactt aaattttctg ttttctgttc ttctttcgtc tttaattcct gacaattttt
    36241 cataataaac agtacgcgag tttcttcctt tgctgctaca tccacaactg tcatttcatt
    36301 ttttgtcaat gtccctgtct tatctgaaca gatcactgtc atagaaccta atgtttctac
    36361 agaaggcagt cctttgatga tcgccttttc ttttgccatt tcatgtactc cgacagatag
    36421 gatcatggtc aacacagctg gcaatccttc gggtaccatt gacaccacca aagcgatcat
    36481 agctgaaaga aggatatgcc aatccatgcc atagcggaat gttgtaaaaa caactaagaa
    36541 caagatcaaa caaagaatgc cttgaaaaat ttgtttgttc aatcggttga tcttcttgat
    36601 cagaggcgtc gtttgttgtt cgaccgattg cagtgcttga ttgattttac cgatttctgt
    36661 cgtgtttcct gtttctacaa cgacaccgac tgcagaacca gattgtacaa gcgttcctga
    36721 aaaagccata tttttttggt caccgattga ttcttctgct gccaaaacgg aatgattttt
    36781 ttcagatgct tctgattctc ccgtcaaaat cgattcttct actagtagat catgtacttc
    36841 gatcaaccga acatctgctg gaacgacgtc tccggcacgt aagacaatga catctccttt
    36901 tactagttct ttcgcaggga ttttttcttg catcccgttc gatagtacta tcgcttcttg
    36961 tcccatcagc tgcttcaaac cattcaacga ttcttctgct tttctttctt gccaataact
    37021 aacgagtccg ttgacgatca ctacgaataa aataatactt ccttcgatgt attcgctcga
    37081 agcaaatttc aaaaaagagg caatgatcaa cacgatcatc agcagatcag taaagtgttt
    37141 tgctattttt ttccatcgtt ttgtctgctg tcttgcttct atctcattat atccttgatc
    37201 agtcaggcgt ttctgccgtt cttcttgcga caagccttct gttgatgttt gatagttttt
    37261 caatacatct tctaccgtca atctgtacca gttcatccta tcccctcctg tttaattgat
    37321 actgttatta tacgcaggga agttttttca ggcgtatcga ttatttcagg ggggtctgaa
    37381 ataattcgtt ttgcagaatg tatctgggcc taactgatat ttctattgct tattctttta
    37441 tatacaaaat ttaagaatac aaaccagata accaataaaa aaggaataaa gcgtgtcgtg
    37501 ctttcgaata acaagctgaa agcaattgaa gtaaaaaaca ccaacaaagc aagatcttta
    37561 tatctttgtg tagtgttccg tttcttttct ttaataaatt gaacatgtgc acacaagatc
    37621 gaagaccaca caaatagaaa acaactcgtt gtcatgcctg ccaaaagctg aaaaaacaat
    37681 cgactatcag ctacaaaaaa atgtagacaa agaccgacaa aaaataacat ccctgtaatt
    37741 aaaataccgt gtaaaggtac gcctttctta gaaagcgcac aaaaccaatg gggcaaactt
    37801 ttctcttttg ctgtttgata taaaatcctg ctagtactgt agactgcact atttgctgag
    37861 gacaaagaag caaccaaaat cattagattg acaattgctg ctccgctttg gcaatggatc
    37921 acttgcagca tagtcactaa aggactttct tttaaatcta aacaatgcca tggaacaaca
    37981 aataaaatcg acaaaatcgt tcctatatag aaaaagagga tacgccaagg cagacagttg
    38041 attgcatttt tcaaattctt ttgtgggtca ctcgtctcac caactgttaa tccgatcatt
    38101 tcaattccag caaaactgaa taaaaccata ggaaatgaag caatcattcc agcaaagcca
    38161 aacgggacta attcattgag ctgtccaaaa tttgctttga ttccaataga ctgggtatga
    38221 aaatctgaga cactcaatcc tatacccaat aagataaaaa ggaaaatccc caagatcttg
    38281 atgattgaga aaaaagattc caattcacca aaccacttga ctgcaacaag gttgactgcc
    38341 attaagatca ttagtgcgct acctccgctt atcaaagctg gtacatgtac aaaccagaaa
    38401 tggatataca atcctattgc tgttaattcc gcaagtccag ctccaagcca gcaaagccaa
    38461 taagtccaat tgacaaaaaa tgcccacttt ttgcctaaat aatagtcaac gaaatcactg
    38521 atagagttaa atgtactatt tgctaaaaat aaatcaccca tgacccgcat caaaccataa
    38581 atagccaaac cgcatatcaa ataaacgaga ataacagctg gccctgctga tgcaattgcc
    38641 tttgctgaac ctaaaaataa tcctgttcca attgcgccac ctaatgcgat taactggata
    38701 tttcgattag ttagagtccg tttcattgcc ggtttttcca tcaagttcac ctttttcttt
    38761 ttcaagcagc atggcgactt cctgttccca agaaggtttc tttatttttt caatacagag
    38821 tggtactaca aacaaaatga cactgatggc tgtgactgca attagattga tccatgtccc
    38881 agctttcatc gtcagtccga cagaaaatcc gattgccgca aataaaacaa taacgacagt
    38941 ccaaactcca agattgctct ttttctcttt ttttccaatt ctaaatggtc gattcaactt
    39001 tgcatgtgta taacgcaatc tcaaaactgc gatccccatg atgacataga cgatacagta
    39061 gataacagta gttgaattta cgagcatcaa aaatgcttga ttgacgccag gaatcagcaa
    39121 ataaaaaagt gcaaagattg agataatgac agactgagtc aaaataacgg atttggatac
    39181 gtcaaattga ttcgtcttcc aaaaattgaa ttttggtgga tacgcacctt ttctagcact
    39241 ttctgtcact gtctttgctg gtcctgaggc ccaagcagat aattggactg ctacaccaat
    39301 gaagacaagt aaactaaaca gattcacaat aagatgtggc aaaccaagaa tttggcaata
    39361 aatcgaaata gattgcgcga tattattcaa ttccatttgt ccttttggta cgacatttgc
    39421 tacaactaaa gcattcaaaa tattgacgaa aaatataaaa attaacgcgg caaatactcc
    39481 gcgaatatac gttttaactg gatttttcaa ccgagtgata tacacagagg acatttcgat
    39541 accagtaaaa ataaacatga tcggcgcaaa ataaacaaat gaggatgcag tcgtagtatc
    39601 tggtattaat ttgtcccatg aaaaactgcc taatgttccg gttggtacga tcccaacttt
    39661 gatccacgca gcaaacccta ataagagcat catcgtcaaa ggcacgtaca atcctaacca
    39721 aacaccgact tttccgccga tttttgccat atcaaatttc aaattcaaaa aagtgatgat
    39781 ccaataaacg actaaaatga caactaatgt aaatttgctg ttcaaaccaa gcggcacatt
    39841 atcgataata ttaccaaaca acggagcaag tgctgaagcg accatcacca tcccaggaaa
    39901 catttggacc cataacagcc atgagacaac gaacccccat ttttttccta gagcattcga
    39961 tacccaaagt tctggaccac ctttttgggg aaacattccg gcaaattcac cagagatcaa
    40021 tgagattgga aaagcgtaca ataatactgc aaccagcatg taaaaaatca tcgtccagct
    40081 cgttgctgct acatctggaa tatttcgtac acttgctaca agggcgcagg tcattccgat
    40141 aaaggtcatg gtcgtgattt gctttgatga accagtatct ttcatcttta ttccttcttt
    40201 caatagcgac atatgttttt gttttctttt atttttgttc aagaacagaa atgacaaggt
    40261 ttcatcgatt cagtacaaaa cccagtatca ccgaaacatc cattgaacga tcttagtgaa
    40321 ctgggtttga tacatgaaac gaacgatcca acttatagaa cgttttataa aaaagaatat
    40381 cttggcgagg aatatatgaa aaacaggggg aaatacaatt gttttgatga agcaaataat
    40441 tgtctgttct ccatatgtct cttgttattc tttcacacaa aaatcagttc tattggatga
    40501 tttcgtttag tttcttttcc attgctttgg cgatttcttt tgtataaaaa ttgaaaattc
    40561 gatcatcatt caaataagga gtcatgatag ccgcacgtag cagagtgatt ttctgttctt
    40621 ttttccattc ttctttcgtt agacccattc tttcaacaaa acgaatcgga gaatcaccgt
    40681 aatcttcttg tgtaaatgta gtatgagaag taataaaccg ttcaccataa acatcgccat
    40741 ccatataaga agaccgatcg aacatttttt catttaattc attgatagcg ttcaaatctg
    40801 tacagccttg ttctttgaat acccaattga ccatattaaa atcaggatga ttcaatggat
    40861 agacttcgat tgtttttcct ttaacagtaa aagtcagatg atcgagaaat tctctgaatc
    40921 tttgagctgc ctcgattgaa gccccaatca attgaccata tccggtcaca ttcaatggca
    40981 atacacgatg tgctgtccag actgcggcgg cggttgctcc agcttttgat ccttccaaaa
    41041 tataagctcc taacatatct ggagccttta ctgatttttc aaacacatat ggcgcgaaat
    41101 aagaaatgat atttctcata tttttatgtt tgattacgat tcctccagca gcataaggga
    41161 catagcccat cttatgagga tctatcgtga cagaatccgc ttctgaaata gctctgaacc
    41221 cttcatatac ctctttgtca atcgttacgc agtggtggaa aacatgatgt tcttcaaaga
    41281 attcagctaa ggaagcatac ggcacaaatt ctcctgcttc gtttagaaac agtgaacgcg
    41341 catagccgcc atatgcggca tcaacatgta aataaaaata gatcccttca tcttttaacc
    41401 tttctcttaa ttggacgatt ttatctacac tgtctacttg accttcttct gtcgttccta
    41461 cgacagcaac cacacctaga attgggattt tttgttcagc taattcacga attgtttttt
    41521 ccaaagcatt aatatccatt cgataatcct cttgaactgg gatagcaacc atctgatcta
    41581 agcctactcc gcaaatatct aacgccttca tccaagaata gtgttttgtt tgaggcacca
    41641 gccatttccc taatctttgg atattttttc cgctacggga agaagctgct ttgacctcat
    41701 ccatttcttc gtccgtaaag ctttcggtca tttccaaaat ttcttcaaca gataaattca
    41761 aaagttccca ctcagacatt tttttcactt gttcaggcag cacttccttg acagctaaag
    41821 gaatcgattt gatgcagcgg gcgtaccata atccttcaag attagcaata gaaccatctg
    41881 ctgcaatgtg tccccatccg tctgtcatat tgaataatga agcaaaatcc tgtcctactt
    41941 ctgcttccat ttgcgaagtt gccatagaag attccaatgc aacattattt gggttccaca
    42001 gcatcgcata gttgtaagct aataaagcag gcatcaatgt ttcagcattc atttgtcccc
    42061 agtaacggcc tgctgaatgc catgggatcg atcctgcgcg taatcgctga ctgacttcat
    42121 ccaagacctc tatcatatgg tcgcgggtgg ctagataatc tggagtgagc ttatctcctt
    42181 cgctaattgc aggcatatct gaaggaatat aattttctct ccatccaaga tgttcatcta
    42241 ccatcttgtt caacaacatt ttataaactg gtccattttc agctttgtcg cctataaaga
    42301 cggccttgat atccatatct ttcatttttc ttccctccat attttgttgg tcaatgtctc
    42361 gcagatacat gtgtttatct gtaacacctc ttcactatag cgccaaaaat gagaaatttt
    42421 tttttataac atcaaatcca cgtagatttt ta
//
LOCUS       DACCQP010000018        42343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCQP010000018 DACCQP010000000
VERSION     DACCQP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.018"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(74..1276)
                     /locus_tag="IVO87_001079"
     CDS             complement(74..1276)
                     /locus_tag="IVO87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7449290.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1393..1908
                     /locus_tag="IVO87_001080"
     CDS             1393..1908
                     /locus_tag="IVO87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7449291.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2107..2553)
                     /locus_tag="IVO87_001081"
     CDS             complement(2107..2553)
                     /locus_tag="IVO87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7449292.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2600..4078)
                     /gene="msr"
                     /locus_tag="IVO87_001082"
     CDS             complement(2600..4078)
                     /gene="msr"
                     /locus_tag="IVO87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7449293.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4432..5061)
                     /locus_tag="IVO87_001083"
     CDS             complement(4432..5061)
                     /locus_tag="IVO87_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7449294.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5045..5509)
                     /locus_tag="IVO87_001084"
     CDS             complement(5045..5509)
                     /locus_tag="IVO87_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7449295.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5996..7264)
                     /locus_tag="IVO87_001085"
     CDS             complement(5996..7264)
                     /locus_tag="IVO87_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7449296.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7495..8232)
                     /locus_tag="IVO87_001086"
     CDS             complement(7495..8232)
                     /locus_tag="IVO87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7449297.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8299..8868)
                     /locus_tag="IVO87_001087"
     CDS             complement(8299..8868)
                     /locus_tag="IVO87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7449298.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8996..9868
                     /locus_tag="IVO87_001088"
     CDS             8996..9868
                     /locus_tag="IVO87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7449299.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10210..11082
                     /locus_tag="IVO87_001089"
     CDS             10210..11082
                     /locus_tag="IVO87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7449300.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11079..13037
                     /locus_tag="IVO87_001090"
     CDS             11079..13037
                     /locus_tag="IVO87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449301.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13369..14220)
                     /locus_tag="IVO87_001091"
     CDS             complement(13369..14220)
                     /locus_tag="IVO87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7449302.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14343..14810
                     /locus_tag="IVO87_001092"
     CDS             14343..14810
                     /locus_tag="IVO87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7449303.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15073..15555)
                     /locus_tag="IVO87_001093"
     CDS             complement(15073..15555)
                     /locus_tag="IVO87_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7449304.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15655..16518)
                     /locus_tag="IVO87_001094"
     CDS             complement(15655..16518)
                     /locus_tag="IVO87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7449305.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17116..17991
                     /locus_tag="IVO87_001095"
     CDS             17116..17991
                     /locus_tag="IVO87_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449306.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(18136..18288)
                     /locus_tag="IVO87_001096"
                     /pseudo
     CDS             complement(18136..18288)
                     /locus_tag="IVO87_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(18556..19887)
                     /locus_tag="IVO87_001097"
     CDS             complement(18556..19887)
                     /locus_tag="IVO87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7449307.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(20036..20749)
                     /locus_tag="IVO87_001098"
     CDS             complement(20036..20749)
                     /locus_tag="IVO87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7449308.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            20924..22348
                     /locus_tag="IVO87_001099"
     CDS             20924..22348
                     /locus_tag="IVO87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449309.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            22647..23354
                     /locus_tag="IVO87_001100"
     CDS             22647..23354
                     /locus_tag="IVO87_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7449310.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            23341..23664
                     /locus_tag="IVO87_001101"
     CDS             23341..23664
                     /locus_tag="IVO87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7449311.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(23753..24790)
                     /locus_tag="IVO87_001102"
     CDS             complement(23753..24790)
                     /locus_tag="IVO87_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7449312.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(25111..26094)
                     /locus_tag="IVO87_001103"
     CDS             complement(25111..26094)
                     /locus_tag="IVO87_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7449313.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(26509..27279)
                     /locus_tag="IVO87_001104"
     CDS             complement(26509..27279)
                     /locus_tag="IVO87_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449314.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(27272..28207)
                     /locus_tag="IVO87_001105"
     CDS             complement(27272..28207)
                     /locus_tag="IVO87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449315.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28523..30172)
                     /locus_tag="IVO87_001106"
     CDS             complement(28523..30172)
                     /locus_tag="IVO87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7449316.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(30289..32892)
                     /locus_tag="IVO87_001107"
     CDS             complement(30289..32892)
                     /locus_tag="IVO87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7449317.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(32904..33953)
                     /locus_tag="IVO87_001108"
     CDS             complement(32904..33953)
                     /locus_tag="IVO87_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7449318.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(33979..34293)
                     /locus_tag="IVO87_001109"
     CDS             complement(33979..34293)
                     /locus_tag="IVO87_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7449319.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(34309..34755)
                     /locus_tag="IVO87_001110"
     CDS             complement(34309..34755)
                     /locus_tag="IVO87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7449320.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(34756..36732)
                     /locus_tag="IVO87_001111"
     CDS             complement(34756..36732)
                     /locus_tag="IVO87_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7449321.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(36890..37786)
                     /locus_tag="IVO87_001112"
     CDS             complement(36890..37786)
                     /locus_tag="IVO87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7449322.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(37779..38459)
                     /locus_tag="IVO87_001113"
     CDS             complement(37779..38459)
                     /locus_tag="IVO87_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449323.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(38460..39242)
                     /locus_tag="IVO87_001114"
     CDS             complement(38460..39242)
                     /locus_tag="IVO87_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7449324.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(39235..40152)
                     /locus_tag="IVO87_001115"
     CDS             complement(39235..40152)
                     /locus_tag="IVO87_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449325.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(40316..40639)
                     /locus_tag="IVO87_001116"
     CDS             complement(40316..40639)
                     /locus_tag="IVO87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7449326.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            41427..>42343
                     /gene="ecbA"
                     /locus_tag="IVO87_001117"
     CDS             41427..>42343
                     /gene="ecbA"
                     /locus_tag="IVO87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HAP7449327.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKT"
BASE COUNT        12815 a         8610 c         7224 g        13694 t
ORIGIN      
        1 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
       61 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
      121 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
      181 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
      241 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
      301 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
      361 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
      421 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
      481 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
      541 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
      601 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
      661 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
      721 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
      781 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
      841 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
      901 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
      961 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     1021 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     1081 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     1141 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     1201 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     1261 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
     1321 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     1381 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     1441 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     1501 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     1561 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     1621 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     1681 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     1741 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     1801 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     1861 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     1921 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     1981 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     2041 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     2101 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     2161 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     2221 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     2281 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     2341 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     2401 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     2461 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     2521 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     2581 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     2641 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     2701 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     2761 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     2821 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     2881 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     2941 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     3001 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     3061 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     3121 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     3181 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     3241 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     3301 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
     3361 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     3421 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     3481 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     3541 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     3601 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     3661 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     3721 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     3781 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     3841 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     3901 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     3961 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     4021 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     4081 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     4141 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     4201 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     4261 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     4321 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     4381 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     4441 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     4501 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     4561 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     4621 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     4681 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     4741 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
     4801 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     4861 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
     4921 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     4981 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     5041 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     5101 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     5161 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
     5221 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     5281 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     5341 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     5401 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     5461 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     5521 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     5581 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     5641 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     5701 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
     5761 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     5821 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     5881 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     5941 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     6001 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     6061 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     6121 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     6181 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     6241 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     6301 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     6361 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     6421 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     6481 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     6541 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
     6601 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
     6661 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
     6721 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
     6781 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
     6841 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
     6901 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
     6961 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
     7021 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
     7081 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
     7141 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
     7201 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
     7261 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
     7321 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
     7381 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
     7441 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
     7501 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
     7561 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
     7621 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
     7681 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
     7741 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
     7801 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
     7861 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
     7921 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
     7981 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
     8041 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
     8101 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
     8161 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
     8221 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
     8281 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
     8341 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
     8401 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
     8461 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
     8521 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
     8581 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
     8641 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
     8701 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
     8761 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
     8821 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
     8881 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
     8941 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
     9001 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
     9061 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
     9121 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
     9181 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
     9241 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
     9301 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
     9361 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
     9421 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
     9481 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
     9541 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
     9601 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
     9661 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
     9721 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
     9781 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
     9841 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
     9901 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
     9961 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    10021 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    10081 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    10141 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    10201 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    10261 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    10321 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    10381 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    10441 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    10501 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    10561 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    10621 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    10681 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    10741 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    10801 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    10861 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    10921 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    10981 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    11041 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    11101 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    11161 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    11221 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    11281 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    11341 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    11401 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    11461 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    11521 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    11581 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    11641 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    11701 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    11761 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    11821 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    11881 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    11941 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    12001 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    12061 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    12121 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    12181 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    12241 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    12301 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    12361 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    12421 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    12481 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    12541 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    12601 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    12661 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    12721 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    12781 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    12841 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    12901 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    12961 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    13021 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    13081 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    13141 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    13201 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    13261 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    13321 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    13381 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    13441 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    13501 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    13561 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    13621 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    13681 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    13741 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    13801 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    13861 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    13921 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    13981 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    14041 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    14101 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    14161 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    14221 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    14281 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    14341 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    14401 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    14461 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    14521 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    14581 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    14641 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    14701 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    14761 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    14821 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    14881 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    14941 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    15001 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    15061 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    15121 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    15181 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    15241 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    15301 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    15361 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    15421 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    15481 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    15541 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    15601 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    15661 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    15721 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    15781 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    15841 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    15901 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    15961 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    16021 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    16081 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    16141 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    16201 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    16261 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    16321 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    16381 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    16441 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    16501 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    16561 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    16621 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    16681 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    16741 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    16801 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    16861 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    16921 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    16981 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    17041 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    17101 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    17161 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    17221 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    17281 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    17341 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    17401 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    17461 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    17521 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    17581 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    17641 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    17701 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    17761 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    17821 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    17881 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    17941 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    18001 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    18061 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    18121 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    18181 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    18241 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    18301 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    18361 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    18421 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    18481 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    18541 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    18601 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    18661 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    18721 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    18781 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    18841 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    18901 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    18961 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    19021 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    19081 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    19141 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    19201 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    19261 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    19321 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    19381 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    19441 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    19501 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    19561 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    19621 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    19681 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    19741 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    19801 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    19861 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    19921 actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt taattaaata
    19981 tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc cgagcttatt
    20041 tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat accgaatact
    20101 cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata aattcacctg
    20161 gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg atacgtatgt
    20221 gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca gatttcttta
    20281 aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt
    20341 atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct tttacactga
    20401 tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta tggatttctg
    20461 aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca agaactcgtg
    20521 agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt cgcaaaactc
    20581 cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta tgataaataa
    20641 ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca tttacaattt
    20701 ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat ggtgaccctc
    20761 caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa atccacatca
    20821 aaattataca cacaataaaa aagatatata tatttaaaca aaactattta ctttaagttg
    20881 taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa
    20941 gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc aagcagaagg
    21001 aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag aacaacctta
    21061 ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc aatataaaga
    21121 agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta tttcttgggc
    21181 acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt ctttctatcg
    21241 aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact tatatcattt
    21301 tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag tagttgatgc
    21361 ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt gtcattggtt
    21421 taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt ttcattatcc
    21481 aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac ttgcacacgc
    21541 aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg ttattctaaa
    21601 tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc
    21661 tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt tttctaaaaa
    21721 gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga aagaagatct
    21781 tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg agccacgtcg
    21841 cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag agtggttttt
    21901 cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg aaatttacga
    21961 aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca tcgaagcatt
    22021 tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg atacagggca
    22081 aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact tatggaaggc
    22141 aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg attgctggtc
    22201 atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta ctactcaaaa
    22261 aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa atggatttag
    22321 atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag tgaacacatt
    22381 agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata cttcatttat
    22441 acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag gtctggaaat
    22501 ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag
    22561 ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt
    22621 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
    22681 aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg
    22741 ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg
    22801 gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg
    22861 ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
    22921 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
    22981 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
    23041 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
    23101 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
    23161 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
    23221 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
    23281 gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
    23341 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
    23401 tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag
    23461 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
    23521 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
    23581 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc
    23641 ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat
    23701 gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt
    23761 tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg
    23821 cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga
    23881 taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt
    23941 catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt
    24001 tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg
    24061 gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat
    24121 actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga
    24181 ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt
    24241 agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc
    24301 cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt
    24361 tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat
    24421 ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat
    24481 tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt
    24541 gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag
    24601 catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt
    24661 tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa
    24721 agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct
    24781 tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat
    24841 ttcaatatat atagtatgct atttgttcca cttttattca tcataattat tctcttactt
    24901 aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca
    24961 acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa
    25021 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg
    25081 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
    25141 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
    25201 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
    25261 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
    25321 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
    25381 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
    25441 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
    25501 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
    25561 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
    25621 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
    25681 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
    25741 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
    25801 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
    25861 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
    25921 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
    25981 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
    26041 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
    26101 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
    26161 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
    26221 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
    26281 cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
    26341 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca
    26401 atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt
    26461 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa
    26521 tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac
    26581 aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa
    26641 accgaagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa
    26701 catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg
    26761 cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga
    26821 aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag
    26881 tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac
    26941 tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc
    27001 ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact
    27061 agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc
    27121 catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt
    27181 gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat
    27241 agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat
    27301 tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa
    27361 gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt
    27421 caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag
    27481 gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa
    27541 gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac
    27601 aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt
    27661 tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg
    27721 gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc
    27781 gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact
    27841 gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg
    27901 cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga
    27961 ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat
    28021 tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagacttcc
    28081 cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc
    28141 ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat
    28201 ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc
    28261 agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc
    28321 taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga
    28381 taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt
    28441 ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa
    28501 taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata
    28561 acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca
    28621 aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc
    28681 cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct
    28741 ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc
    28801 gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat
    28861 ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt
    28921 tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag
    28981 tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg
    29041 ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac
    29101 ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg
    29161 gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg
    29221 gcatataagt acggtaattc aatattgcag cggctacttc taattctccc agcaattccc
    29281 acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga
    29341 taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac
    29401 tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt
    29461 tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg
    29521 aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat
    29581 agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa
    29641 gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc
    29701 caatcttttt actttttcca tccgctagat attgttttgc aaaattatga tctttttttc
    29761 ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag
    29821 caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca
    29881 acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat
    29941 ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg
    30001 tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt
    30061 cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta
    30121 ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct
    30181 ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa
    30241 aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt
    30301 tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag
    30361 tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt
    30421 tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga
    30481 tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt
    30541 ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg
    30601 ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa
    30661 tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag
    30721 cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg
    30781 ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg
    30841 attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt
    30901 ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt
    30961 ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg
    31021 tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta
    31081 aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga
    31141 tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt
    31201 aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt
    31261 agctgtttct aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt
    31321 atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac
    31381 tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt
    31441 tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt
    31501 gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc
    31561 atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata
    31621 aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc
    31681 tactacatgc acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac
    31741 ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc
    31801 gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga
    31861 aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg
    31921 aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac
    31981 tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga
    32041 gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg
    32101 taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg
    32161 gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt
    32221 ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc
    32281 aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt
    32341 catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa
    32401 tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca
    32461 catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa
    32521 cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat
    32581 tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc
    32641 ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt
    32701 ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt
    32761 tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac
    32821 gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg
    32881 tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct
    32941 tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata
    33001 acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa
    33061 agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc
    33121 accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca
    33181 gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca
    33241 taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct
    33301 aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca
    33361 tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc
    33421 gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc
    33481 attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta
    33541 acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt
    33601 ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc
    33661 ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca
    33721 gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga
    33781 ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca
    33841 aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa
    33901 accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac
    33961 tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc
    34021 accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc
    34081 tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga
    34141 aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc
    34201 tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc
    34261 gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag
    34321 agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc
    34381 catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc
    34441 ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac
    34501 agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg
    34561 aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc
    34621 tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt
    34681 ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat ttatattgat
    34741 tttttcactg atcatttata agccttcttt caatatataa actaattttt caaacgacga
    34801 actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga atgtttcata
    34861 aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa cagaatgaac
    34921 aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta ctggcttttt
    34981 tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg aagatagttt
    35041 ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt ttaccaattt
    35101 tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg taaaaaagca
    35161 aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta actgtagata
    35221 ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa taacaggaaa
    35281 atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt ttctatcttg
    35341 tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac cgttatttat
    35401 tctttgctct aataattttg atgttccata tccgctacta caaactaata tcactttcgt
    35461 tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc
    35521 atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat ctacacttat
    35581 cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat agatatttac
    35641 tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat tgatcaattc
    35701 ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct caacacccat
    35761 atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt tttcaatcga
    35821 agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg aaaatgcttt
    35881 ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag aattattact
    35941 acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt ggatagctaa
    36001 agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa
    36061 ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac caatgaaatt
    36121 gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac cccaataata
    36181 gttcacaact tctgacatca accttctttt acttttttca tcagctttga tgcgcacgcc
    36241 atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa catctttcat
    36301 tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt gaatctttag
    36361 agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct ccggagagtc
    36421 tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt ttccaataag
    36481 cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt ggattatttc
    36541 tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt gttctgataa
    36601 ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca tttgccgttc
    36661 gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta caattattat
    36721 ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat attcttgatt
    36781 atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc tctattaaat
    36841 atatataaga taatacattt ttctactagt tctcactaat cttgtataat taaaccctcc
    36901 gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc tctcctccca
    36961 ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc gtatttgtgc
    37021 ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt tgcgttcctg
    37081 gtttgatagg attggccact gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct
    37141 ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc actcgcagca
    37201 acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac gctacctcga
    37261 tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc tctttagaaa
    37321 aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca agatcataaa
    37381 actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt aaatacttct
    37441 cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta atagcagtca
    37501 aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt tcttccattt
    37561 cttgaaatgc ttgtcctttt tgttcatttt catccaacca tgtattcagt gcatctacta
    37621 ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat tcttgtcgtt
    37681 cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg aagaaaataa
    37741 aacttcccat aaagataaca acgagaagca acagccattt aatcaaatct aaaccccact
    37801 ccccacaccg tatgaatata cacagcatgt gcctttgcta gcttccctcg aagattggaa
    37861 atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac tgtttcataa
    37921 agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag cagatcgaat
    37981 tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga ctctttgtcc
    38041 agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac agaagtctga
    38101 tgacgaagtt ggatgccgat tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca
    38161 tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct ttttgctgaa
    38221 agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa
    38281 agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct taacaataat
    38341 tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag aacctcctcc
    38401 agtaaggaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag caggaccacc
    38461 tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac cacgattgtt
    38521 actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc
    38581 gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc tttgattttt
    38641 gcgaaaacac cgggaatgaa ggccgttccc atgatataaa gaaaagaaag aagcaaactg
    38701 acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt ccctaatgtc
    38761 agcagaagca atacaggaaa tacttttaga acataatttc catagttttt gattccctca
    38821 acttcaccaa atccgaaagc aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc
    38881 acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata agtcattcga
    38941 ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc cccaatataa
    39001 aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc taccattaaa
    39061 atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt gccgtatttt
    39121 gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat cagtcctagt
    39181 gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa tagattaaac
    39241 atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc caactaggtg
    39301 aataccatcc acaggaactt ttgcttctac caatgcttga ttgatggctg ctacttggtc
    39361 aattccttca aaaagctgga taacacggct agattgtaca aaataatccc tataaccaag
    39421 agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta taaattgacg
    39481 agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt catgcaaaat
    39541 accatagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa gcaagacggt
    39601 gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca tccctgctgg
    39661 atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga tcagtgcatt
    39721 tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct ttttccctgt
    39781 attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca caccgccgtt
    39841 cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag caggggtttc
    39901 aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat
    39961 cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag acttacccgc
    40021 tccattttcc ccaatcaatc catagatcat tccacgtttg atttcgattg atactttatc
    40081 taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga ccgtttcatt
    40141 tccaaaatcc atacttatcc ttctttctta ttgatgagtg aagtatagtt gacgggtata
    40201 aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa
    40261 acaatcggac aatacgtgtc tttcctatta tcaggcagtt tgttatacgc ttgtatcagt
    40321 ttcctgattc tttcttttcg accaaagtcg tttctctgta ttcctcttca ctgaaaattt
    40381 ttgcttcttt cagatcaagc ttgagtaatt ttaccagacc ggttataagg ataactatag
    40441 caagactccc tcccagaaaa attagactgt ctcgcagacg tggttgcgtt gtcttccaat
    40501 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    40561 tcgttgacat aagtcgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    40621 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    40681 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    40741 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    40801 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    40861 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    40921 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    40981 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    41041 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    41101 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    41161 cattgaataa gtttgaaaaa ccttgattta taaaggtttt cccgtaaata tattttatta
    41221 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    41281 tttcgtattt tagattaaat ttttaggctc attcattctc aaatccattc tattcagtca
    41341 ataaaaattt taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg
    41401 tgcttaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat
    41461 taactatttt gagtttggct gttcaagcaa ttgttggagc aattgctcct acgattactt
    41521 ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat
    41581 atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt
    41641 atgcagtgta caacggcttt aaacaagata ttttctgtat tgaaccagga gtcccgattt
    41701 acaatgagtt cactcctggc tatgagaaaa acccgttacc agatatgtcc gataaagcca
    41761 aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac
    41821 agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg
    41881 gtagtgccgt aaatatcgca gccattgagg caaaaataaa tcaagccatt gacgattatc
    41941 agaagaaacc aaccttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg
    42001 tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa aatgccgcaa
    42061 acattgatta tcgtgtgaat ggtaatcagt tgataatcac cccaaatgcc aattcaaaag
    42121 aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa
    42181 aggctggcca acagacttta atggccgggg cgattgataa acctaatacc tacacaatca
    42241 aaattgatgt ggaaacggag ggttctttaa agatcaaaaa agtcgataaa gaatcgggtg
    42301 atgttgtacc aggaacggtt ttccatttag actttggaaa aac
//
LOCUS       DACCQP010000019        39967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCQP010000019 DACCQP010000000
VERSION     DACCQP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.019"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(367..933)
                     /locus_tag="IVO87_001118"
     CDS             complement(367..933)
                     /locus_tag="IVO87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7449328.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(933..1889)
                     /locus_tag="IVO87_001119"
     CDS             complement(933..1889)
                     /locus_tag="IVO87_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7449329.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1889..2545)
                     /locus_tag="IVO87_001120"
     CDS             complement(1889..2545)
                     /locus_tag="IVO87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7449330.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2915..4933
                     /locus_tag="IVO87_001121"
     CDS             2915..4933
                     /locus_tag="IVO87_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7449331.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5360..7774
                     /locus_tag="IVO87_001122"
     CDS             5360..7774
                     /locus_tag="IVO87_001122"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7449332.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8122..9675
                     /locus_tag="IVO87_001123"
     CDS             8122..9675
                     /locus_tag="IVO87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7449333.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10056..11840)
                     /locus_tag="IVO87_001124"
     CDS             complement(10056..11840)
                     /locus_tag="IVO87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449334.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11855..13576)
                     /locus_tag="IVO87_001125"
     CDS             complement(11855..13576)
                     /locus_tag="IVO87_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449335.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13676..14437
                     /locus_tag="IVO87_001126"
     CDS             13676..14437
                     /locus_tag="IVO87_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7449336.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15404..15672
                     /locus_tag="IVO87_001127"
                     /pseudo
     CDS             15404..15672
                     /locus_tag="IVO87_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16344..19466
                     /locus_tag="IVO87_001128"
     CDS             16344..19466
                     /locus_tag="IVO87_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7449337.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
     gene            complement(19890..20060)
                     /locus_tag="IVO87_001129"
     CDS             complement(19890..20060)
                     /locus_tag="IVO87_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7449338.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20254..20433)
                     /locus_tag="IVO87_001130"
     CDS             complement(20254..20433)
                     /locus_tag="IVO87_001130"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449339.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            20820..21932
                     /locus_tag="IVO87_001131"
     CDS             20820..21932
                     /locus_tag="IVO87_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7449340.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22010..22303)
                     /locus_tag="IVO87_001132"
     CDS             complement(22010..22303)
                     /locus_tag="IVO87_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7449341.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            22471..22785
                     /locus_tag="IVO87_001133"
     CDS             22471..22785
                     /locus_tag="IVO87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7449342.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            22806..24785
                     /locus_tag="IVO87_001134"
     CDS             22806..24785
                     /locus_tag="IVO87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7449343.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            24798..25124
                     /locus_tag="IVO87_001135"
     CDS             24798..25124
                     /locus_tag="IVO87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7449344.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25140..25658
                     /locus_tag="IVO87_001136"
     CDS             25140..25658
                     /locus_tag="IVO87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449345.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            25658..27010
                     /gene="celB"
                     /locus_tag="IVO87_001137"
     CDS             25658..27010
                     /gene="celB"
                     /locus_tag="IVO87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7449346.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27094..28599)
                     /locus_tag="IVO87_001138"
     CDS             complement(27094..28599)
                     /locus_tag="IVO87_001138"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7449347.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(28691..29281)
                     /locus_tag="IVO87_001139"
     CDS             complement(28691..29281)
                     /locus_tag="IVO87_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7449348.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            29460..31106
                     /locus_tag="IVO87_001140"
     CDS             29460..31106
                     /locus_tag="IVO87_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7449349.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31155..31874
                     /locus_tag="IVO87_001141"
     CDS             31155..31874
                     /locus_tag="IVO87_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7449350.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32353..33300
                     /locus_tag="IVO87_001142"
     CDS             32353..33300
                     /locus_tag="IVO87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449351.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33312..34232
                     /locus_tag="IVO87_001143"
     CDS             33312..34232
                     /locus_tag="IVO87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7449352.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34273..35730
                     /locus_tag="IVO87_001144"
     CDS             34273..35730
                     /locus_tag="IVO87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7449353.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36075..36689
                     /locus_tag="IVO87_001145"
     CDS             36075..36689
                     /locus_tag="IVO87_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7449354.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            36682..38406
                     /locus_tag="IVO87_001146"
     CDS             36682..38406
                     /locus_tag="IVO87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7449355.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            38403..39854
                     /locus_tag="IVO87_001147"
     CDS             38403..39854
                     /locus_tag="IVO87_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449356.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT        13006 a         6923 c         8080 g        11958 t
ORIGIN      
        1 ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat
       61 aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat
      121 atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg tgaaaaagct
      181 gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg
      241 ttggacttat taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc
      301 aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt
      361 ttttatttat ctttttttct caatacaata taaaataggc ggctggttct tttggttgat
      421 aaaccgataa ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac
      481 catatccagt tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc
      541 gcgaggcaca agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt
      601 gatgattgct ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt
      661 ttgttgttca gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg
      721 ttcttctaac tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat
      781 atcaaaacta tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg
      841 gccattgccc atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa
      901 tgtatggctg aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca
      961 tattttcctt ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc
     1021 cattttttca agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa
     1081 cgatggatga tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag
     1141 tcaggacggc tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg
     1201 cgcatcagat gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt
     1261 ctgacatttt ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta
     1321 caaacacgga tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca
     1381 tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc
     1441 cataaataca tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa
     1501 tccggtcgtg taccgactga taatcctacg acacctggta aattgactac ttgctcaaat
     1561 cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg
     1621 tattgttgga catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt
     1681 ggaagagggt cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta
     1741 caaccgccat gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc
     1801 accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt
     1861 ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta
     1921 tttgttttct tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta
     1981 agtagccgcc gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga
     2041 cacttactat aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa
     2101 caaaagctgg aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc
     2161 cactagggaa actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca
     2221 tgaaaaatag tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga
     2281 accaaaatgt ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata
     2341 aaatgatgac ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat
     2401 caggataaga agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca
     2461 gatggaactt tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca
     2521 cctgaaatga tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc
     2581 atttttggtc cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga
     2641 ataaccaata ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca
     2701 aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag
     2761 acgtaggaag aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt
     2821 acaaaaaaca acaaagctct taaataactt ttaataaata ttaagagaag ttataattat
     2881 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
     2941 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
     3001 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
     3061 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
     3121 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
     3181 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
     3241 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
     3301 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
     3361 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
     3421 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
     3481 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
     3541 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
     3601 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
     3661 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
     3721 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
     3781 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
     3841 taattccggt aacaatacca actcttcagg aaatgctggt acatcaaacg gaggacaaac
     3901 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     3961 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     4021 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     4081 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     4141 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     4201 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     4261 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     4321 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     4381 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     4441 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     4501 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     4561 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     4621 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     4681 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     4741 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     4801 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     4861 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     4921 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     4981 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     5041 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     5101 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     5161 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     5221 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     5281 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     5341 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     5401 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     5461 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     5521 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     5581 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     5641 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     5701 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     5761 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     5821 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     5881 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     5941 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     6001 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     6061 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     6121 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     6181 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     6241 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     6301 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     6361 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     6421 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     6481 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     6541 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     6601 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     6661 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     6721 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     6781 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     6841 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     6901 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     6961 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     7021 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     7081 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     7141 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     7201 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     7261 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     7321 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     7381 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     7441 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     7501 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     7561 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     7621 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     7681 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     7741 accaaacaag ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat
     7801 gagttgtgca aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt
     7861 atttcaaatt gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt
     7921 tcaatgtctt tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt
     7981 ctattaatct tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat
     8041 tcataaattt tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa
     8101 gtatgagaaa aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct
     8161 aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta
     8221 agcttagtat ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat
     8281 atgagtgcag tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt
     8341 tcgtggggag ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg
     8401 ctccgttacc atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag
     8461 tttttacata ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg
     8521 acgagtcaag cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat
     8581 gatttaccta tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt
     8641 tttttactag aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg
     8701 acaactaaga tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc
     8761 tattatggga gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg
     8821 acaatgaact caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat
     8881 cctaagcaaa aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag
     8941 acgcactctc tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat
     9001 tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat
     9061 tggatggaca acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg
     9121 gatgatgtac gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc
     9181 acccaatcag ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta
     9241 tttatctgta catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg
     9301 gatggcggaa tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat
     9361 gtcagcttcg ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca
     9421 aacaacggag gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca
     9481 ggggggatag atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta
     9541 gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc
     9601 ctcttccgtt caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta
     9661 gaacaaggag attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa
     9721 acaaattcaa aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg
     9781 acaagagcaa caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct
     9841 tattgccgat aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc
     9901 ttttgtcact acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta
     9961 ttctttgtgt ttctagtggc accactagta catttaaaaa agctatcgat catggactaa
    10021 tcatacatga tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta
    10081 taaagactgg cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg
    10141 atatcgccat tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt
    10201 gcaatcacaa aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct
    10261 tctgttcgtg tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct
    10321 agaatgactc gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg
    10381 ctagaaatga tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg
    10441 atttcagccg cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta
    10501 tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt
    10561 agttctccac gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc
    10621 agttcataaa aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca
    10681 acgatcgcta ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta
    10741 tctgatgtgt aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa
    10801 agtcttgcat ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta
    10861 tcgattgctg ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt
    10921 tcagaaatct gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct
    10981 gatagaacaa gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa
    11041 cgaatggccg gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc
    11101 tttttattta cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc
    11161 actagattac cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga
    11221 ttctgttcag aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca
    11281 acgatcagca ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca
    11341 gcagcagcta ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga
    11401 gaaagttgat tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca
    11461 aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg
    11521 atcttttcgc ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag
    11581 actgaacagc cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa
    11641 gaaagctgtc caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc
    11701 atgatcaagg gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg
    11761 taaaaatatt tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc
    11821 aacgtttttt tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact
    11881 cctgataagt ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc
    11941 cttcatctaa aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa
    12001 tcacgagagt tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt
    12061 tatagtctaa agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg
    12121 ctcgagcaat acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa
    12181 caaagctgtc caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga
    12241 tcgctttaga caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa
    12301 ttgtcccgct gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt
    12361 cttgagtcat ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac
    12421 taagtctcaa gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg
    12481 ctgttgtttt tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt
    12541 cagtatattt aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt
    12601 cagctactgt tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta
    12661 tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca
    12721 agtaagcaag tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg
    12781 ctccttctgc tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga
    12841 gcaaaaaagc aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag
    12901 catagtcagc aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac
    12961 ggatcatatt aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg
    13021 gctggatact caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat
    13081 aaaaaacaac agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga
    13141 gaatggtgaa aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca
    13201 tttgctggac attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac
    13261 gactgatatc cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga
    13321 atccaaacct agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc
    13381 ctagaaacgc tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac
    13441 ttgctatacc tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga
    13501 tttctaaaca gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg
    13561 tttttaagat agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc
    13621 aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa
    13681 aaaaatgtat acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg
    13741 ctattgggat gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt
    13801 gtttgatatc gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat
    13861 accgatcaaa cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact
    13921 tgatgaaaca gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga
    13981 gataagggag caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc
    14041 tgtagaagag atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat
    14101 caaaaaattt ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc
    14161 acagacgatg tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt
    14221 gatttgggag aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa
    14281 ccatgcggaa cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa
    14341 aacaggagaa gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac
    14401 tgaatattat catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc
    14461 gaaaatgatg gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg
    14521 tgggggatta ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta
    14581 aaaatggaat ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt
    14641 atggaaaaat aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga
    14701 agaaaaattc atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg
    14761 tgcataaaag cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa
    14821 ggcaaaaata aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg
    14881 actttcctca gctatcggtg atactaccga tagcattttt ttgcttataa aataattata
    14941 agtatttaga gatttttctt ctaacattct tcaaacaaga aaaatcacaa aggttattgt
    15001 tatttttata tattcttttt aagaatgatt gtttttttat agaaatttta aaaaataaaa
    15061 cacgttattg atggatagtt agtttgttat ttggtaatat tatagaagtt gactggtagt
    15121 gatttatttt tcgtcgtttt ataaaaatgt tattttttgg aaaaattcac aaatatgtaa
    15181 cgaatgtttt tctggaattt gctgtgttaa aaagcagtat gactaatata aatactattg
    15241 tcatttatgc aattttttat ggatagtaat tttagagggg aatcaatgaa ggaagaatag
    15301 aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata gcaatacatc ttcaagcttt
    15361 aaaattcgag agaatcatct tggtagtgga atcaactaca acaaaaggat taaaccaatt
    15421 aatagataga attaccaatg gaaaaataga aaaggtagtt attctataca aaagttgttt
    15481 aattcgcttc ggttatgaac ttagtgaaac cctttgcaat atatggtaca gaatatagaa
    15541 attattgaca ttacagaaaa tgatgaagat ttaatacaga ttgtcactat ttgtagatac
    15601 cttttacaag gaaaccgagc caataaagct aagaagatga ttaaggagtt gatggaagac
    15661 gatacaagtt aagaaagtga aactacgccc aaataaagag caaaaagagt aattggacgt
    15721 ggaaaatgca agaaatgaac catcgattcg gtggacagtt tattagtaat gacggtttga
    15781 gaacatccat gacgagaatg aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa
    15841 ataatatggc caaataagcg atcagttatc gatgaatccc aagtattcta aactatatat
    15901 tcattttgat gctaagcatt gatatatttt tttgggattg atgaaggtta accaatacag
    15961 ggactcactg atgaaattct agagtctgac cttggtatca acgatttagt gattgtttct
    16021 aatggaaaag tataaaagta tataagtaca ttaacaaaat aactattagg gttacgctcc
    16081 agcttgtgca aggcactggc aggaggaatc gtgaaatcat aattatggtt aagccacacg
    16141 gacaactaaa agtcatacaa agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt
    16201 agctttgttt acagcttaca ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa
    16261 cgcagccatt ttgagttggg gaatcttttt agaaaattat cgggtacctt gttgtttagt
    16321 caaaattata agggggagta atagtgagga acaaatttca taaactgtta agtatgtttc
    16381 tagttatgct tattgtgatc aatcaatttg ggaacataac ggttctagct gagtctttag
    16441 cagaacagcc agagcgttcg ctgacgatcg aagttgatca agaggaggct gctttgggtg
    16501 agcagttcca gttgcgagtc agaggaacac cagacctgct aaatgcattg gtcattgata
    16561 agagtgagga tttgattgaa ggggaagtga agcaactcaa tgattacgaa cgtctgatta
    16621 cttattcttc ccagcattca ggtaaatacg aattgaccgc aagaagcggt gatattcaat
    16681 caaatacggt ctcaatagaa atcagtgtac cagaagaaag cacaagtgag tcttttaatg
    16741 taccagaaga aagcacaagt gaatctttta atataccaga agaaagctta agtgaatctt
    16801 ttaatgaagc cagatcatct gagccatcta taacgcagga atctcagatt gaaagacaag
    16861 aggagctctt gaattatgac atcggcatct ctgctgagcg ccccactttg ccgagcaggc
    16921 atacacaaac gttaaaggga tacattcaag gaggcgctgg cgatattctt gaagaatatg
    16981 gggagatggt cgtacgagtt cctagagcat cgattggctt aattgagttc attagagtgg
    17041 aggtcgctga tgattcacct ttaaagccac tggcatttta ccaagaaggc acagacgttg
    17101 tttttccatt tacctttgat tatgaaggat taaatgaagc cgctcagtcg tatacattca
    17161 gtatcacgtt tgaagttgct ttctttcaat caggcggtac tgagccaaat aatgactttc
    17221 aagtgagtct tacttatgct gagcgagcag caaacacgga attttcaatt acgcctattc
    17281 cttcatcaag acccattttt tcgaaattat actatggctc tagagaaatc gacagccaag
    17341 gaaatcttgg gaatctgctg attgacgggc aagacgtgac caacagtaat aaatttattt
    17401 tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt ggttaccgat gtactaccgc
    17461 aaggaacaac tttggcaccg aatccgacta catggagttc cactggaaat agagaaccta
    17521 tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg tgaagcaaac gttagaactc
    17581 attatgtttc cgaggaattt gtggacaaag tatcttttaa taaggaaacg aatacacttg
    17641 ttgtggactt tggtaatcta gcagagggtc aatcgtattt tattgaatat gctcttgaag
    17701 tgaccgatgt cgacttgttc aatgaaggaa gacaagagga tgggttttct cggaatcgag
    17761 cgagtatgac ctacaataat caggtaatga gccgagacta ccgcttgaga atccctgaaa
    17821 gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg
    17881 aaaatgaact aacgtatact ctgcatgtct cgatgttttc tggaacaaga aatacgcttg
    17941 ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc atttctatca gatattccct
    18001 ctttcattga tgccgaagtc aataatggaa tagttcgttt cagtgcgaat aatacgattg
    18061 gtggtccgac aagttttacc ctctctttta aggtcaatgt agaggcgtat gcggtcggcg
    18121 atcaaatttt aaactttgca acgcttgtga atagtgacaa tcgattgaat acgagtactg
    18181 taagaacaag gaaaatcgat gggcgtatta aagtaattaa aactgatgag aatggccaaa
    18241 ggctggcagg agcaaaattt gaaattttat caagcgattc acaagtgata caagaaggta
    18301 taacggcaga aaacggagaa tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc
    18361 gagaaacaga agcaccggaa gggtatcttt tagatgcgac ggtacatgat gtttttataa
    18421 atgaatactc catagaagaa gtgacgttaa ccattgaaaa catccttgat acgggtggtg
    18481 ttgaactaat taaacatgct gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt
    18541 tacagaaccg agaaggagaa acgctacaaa ccggtttaac tacaggagaa gatggaaaac
    18601 tggcaattga cggtttagca ccaggagcat atcagctggt agaaactcaa gcgccaatag
    18661 ggtatgaatt agatgcgaca ccaatcgagt ttgagatcga aagaagtcag acagcggtgg
    18721 tagaattgac gaaagaaaac cgacttacac caggcggtgt cgtgttgaca aagatcgatg
    18781 accaaagcgg ggaaatcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg
    18841 ttctgcagtc aggtctaacc acaggagcag atggaaaact ggcgattgac ggcttagcac
    18901 caggagtgta tcagctggta gaaacccaag cgccaacggg gtatgaattg gatgcgacac
    18961 caatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagaaaatc
    19021 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcgga gaagtcctgc
    19081 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
    19141 caggagcaga tggaaaactg gcgatagatg gcttagcacc aggagcatat cagctggtag
    19201 aaactcaagc gccaacgggg tatgaattgg atgcgacacc agtaactttc aagatcgaaa
    19261 aagaacaaga agtggcgatc tttttaacaa aagagaatag aaaagttgct gatagctcag
    19321 atataagagg aaacaatcca acaagcagtg gaaataagca tcttccaaaa actggagaaa
    19381 ccatcattag ccagtttata ttgtcaatat tgggtgtgct acttgttttc atcagtataa
    19441 aatttagaaa gaaacgaaat atatgagcta aaataaaaaa ttgaataagc atagttttta
    19501 aaatcaacta gtacccatca attgaatgga aactaccgat tttagaaggg gtatacaaac
    19561 aaatattagt atcggagtag agaaggttga acccaaccag taaataagta aagacaattt
    19621 tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa tactagttaa taaaaaatat
    19681 acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt tttttgccta atgactcaag
    19741 tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt tgtaattttt
    19801 tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc ttttgtctcg
    19861 gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt ggaaaaacca
    19921 agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag ctgaaagtag
    19981 gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca gctaaggaaa
    20041 tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat ttttctgtct
    20101 cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa cgaaattcac
    20161 aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc ttctcaactc
    20221 tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa taaaaaataa
    20281 cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg aaatcgagtg
    20341 ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt tacaggtata
    20401 gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa agtggacaat
    20461 ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta ccacagcaga
    20521 accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga tcaatgtttt
    20581 tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt ctattgatct
    20641 cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg tttattttgt
    20701 ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa taggaaaaat
    20761 agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac ctgtcataga
    20821 tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt tatcagtctc
    20881 tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct aaaaaagaaa
    20941 agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg tttgccattt
    21001 gttttatcat tataattaca acattatttg aatcgaagca taaaccatac gctgtggttt
    21061 tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt tcccattcga
    21121 tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc cagctgatca
    21181 aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata ttaatggcaa
    21241 ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat ctctttattg
    21301 tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca agtagcttat
    21361 tatttttgtt tttttgttgg ttttcagttc tcctttacag aaaacatcga gaaaagaaca
    21421 aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag gaaaaaattt
    21481 ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag tatgtccctt
    21541 ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc tatggattga
    21601 tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg attggatgtg
    21661 ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata ttaggtggtc
    21721 tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct tttggtgctt
    21781 tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc atttttgaga
    21841 atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata cttatccgaa
    21901 aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa taaggaaaaa
    21961 gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt tacggtaaat
    22021 cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg tttatttgag
    22081 ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa cggtcgatca
    22141 gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg tttccttctt
    22201 ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat tttctggctt
    22261 gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc cctcctgaaa
    22321 aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat gatacacatt
    22381 ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa gatatgcttg
    22441 gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat ttgtgcggca
    22501 ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca aaaaagagaa
    22561 gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa gaatagtgat
    22621 tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt caaaaaatta
    22681 ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt cccaatccct
    22741 acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa taggagtgga
    22801 tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac aagaaggagt
    22861 atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca atccgtaatt
    22921 acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt gctaaaccag
    22981 gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt ttgagtcggc
    23041 aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc gaagatcgac
    23101 aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg gatgatttgg
    23161 cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa atcaaggagc
    23221 gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca tggatcgaag
    23281 gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac cattatggaa
    23341 attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt tttgaagagc
    23401 tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg gattttatca
    23461 tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa gggtttgaaa
    23521 tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa gtggcgcagc
    23581 ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa gaaattgctt
    23641 atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat gatcaggaac
    23701 tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat ttttcattag
    23761 tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg atgattcgat
    23821 tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa gaaaatccca
    23881 aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa aactatacca
    23941 tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg gaaaaagaaa
    24001 gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc agtgcccaac
    24061 tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta agtgtaaaag
    24121 gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt tcttctattg
    24181 atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt ttgaatgaag
    24241 atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat tcctcagata
    24301 gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa cagttgtctg
    24361 aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt catgatttgc
    24421 tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga aagcagatcc
    24481 agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc ccacatcccg
    24541 ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct gggatgcagt
    24601 gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat gtcgaaaatg
    24661 aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta aaagtacagc
    24721 aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc aaactgatcg
    24781 aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa gtgacttctt
    24841 ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca tttcgctcga
    24901 tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat gaagagattc
    24961 ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg acaaaaatag
    25021 aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg acattattgg
    25081 aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga aggatcattg
    25141 tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt ttgaaaaaca
    25201 tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt ttcatgaaca
    25261 tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta ccgatgattc
    25321 tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga aaacaccaaa
    25381 cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat ttattagtga
    25441 ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca aataacaatg
    25501 gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt gcatttatac
    25561 agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt attaaacaat
    25621 atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt tgcagaaata
    25681 cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg tcatggcagc
    25741 ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt taaatatttt
    25801 gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca aagttagtga
    25861 cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact ttgccattgt
    25921 gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg ttaatccgct
    25981 cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat tgatcatgac
    26041 tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag taaatggtat
    26101 taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag ctattattat
    26161 gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg tcataaaaat
    26221 gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc caacatttgt
    26281 cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa cggatatctt
    26341 caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat ggcttggtgt
    26401 catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg gagcgaatat
    26461 catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg cacaaggcgc
    26521 aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg gatcaggtgc
    26581 gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc tgaaagtgtt
    26641 aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca ttttcgggat
    26701 gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga tggcttctgc
    26761 tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg cacagatgcc
    26821 gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt ggaaagctgc
    26881 tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat ttatcaaatt
    26941 ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag ctagtgaaaa
    27001 gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc ctttttttct
    27061 tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc gcttcacgat
    27121 tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg gatccacgcc
    27181 ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc gtcgaatcgc
    27241 ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg cttcattgat
    27301 ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca aaaaggcgat
    27361 gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg ctttgtttcc
    27421 tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg caaatttcag
    27481 caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg ctccattgga
    27541 ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt cttgtgcatc
    27601 ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat tttcttggtt
    27661 gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc gatcttcatg
    27721 agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat aattgctttc
    27781 ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt ccctaagtaa
    27841 atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga tcaattgtcg
    27901 cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat ggtcaggcga
    27961 gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg ttttgtaagc
    28021 ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt ctgacatcat
    28081 acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg ttttgccatc
    28141 taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt gatccaagat
    28201 tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc cgatcacttt
    28261 caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg ctttacggat
    28321 atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata cataatattg
    28381 aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc ctttacagaa
    28441 aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa attcagcttt
    28501 ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata gattttcttt
    28561 taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc tcgctttatt
    28621 caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc cgctatgaca
    28681 aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt attttcataa
    28741 acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat ccaaaaacat
    28801 aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc attggaagcc
    28861 gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag gccccgactg
    28921 tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt gaaaagaaat
    28981 gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa agcagacttc
    29041 ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga agcagcatca
    29101 taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt tcaactaaat
    29161 gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac caattcaatc
    29221 caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt tcttgtgtca
    29281 ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact ttaccaaaaa
    29341 aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa tcgattgcca
    29401 gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga atctgaccga
    29461 tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa atggcacgag
    29521 gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct gtctatatca
    29581 ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta ttcaatatgg
    29641 gatacaatat atatgcattg tttctactga tctctacagc aggaatacct gctgcgattg
    29701 caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga cgattattta
    29761 tccgtgcatt acagatgatg ggcgggttag gcctgttgtt tgctttgttc atgtacatcg
    29821 cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca acgatgcgtt
    29881 ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg tattttcaag
    29941 gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg gcacgtattt
    30001 tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac tatgtgacgg
    30061 cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt cttgtccttt
    30121 tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac agcgaaaaca
    30181 aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg attccgttta
    30241 ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact tttatgaaga
    30301 tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt tcgattttta
    30361 gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg atcgcgtcta
    30421 ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt ctggcaaaat
    30481 tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca tttggtgtta
    30541 tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta ggaagtaatg
    30601 tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta gtttccaaca
    30661 tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt gggtttctag
    30721 tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc cctttattgg
    30781 cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc catgaagtag
    30841 cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt cttaccttga
    30901 ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta aacgaagata
    30961 gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct ttagtgtatg
    31021 gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga atagcgcgtt
    31081 tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat gctcttttga
    31141 aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt ggcagccgca
    31201 aagaagtcaa aggattaatc aaaaaaggaa cgatcgaagt caatggacaa gtggtgaaaa
    31261 gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga gaaatcatta
    31321 cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt tctgctactc
    31381 aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat cgggaagatc
    31441 tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta acgaatgacg
    31501 gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa tatctagctg
    31561 aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga ctgattattg
    31621 atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat caagagacag
    31681 aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt aagcgaatgg
    31741 tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga aaattttggc
    31801 tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag ctagaacaag
    31861 tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg tagcgccatc
    31921 tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt tcggcttatt
    31981 tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg acaagacttt
    32041 tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca cgatctccct
    32101 aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca atcagctgac
    32161 cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt ttttttctct
    32221 aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc attttcccta
    32281 tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga atgaatcgaa
    32341 aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa tctatggcgt
    32401 tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt tttcttttac
    32461 atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc agatgggttc
    32521 ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt tttattttct
    32581 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
    32641 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
    32701 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
    32761 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
    32821 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
    32881 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
    32941 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
    33001 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
    33061 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
    33121 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
    33181 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
    33241 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
    33301 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
    33361 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
    33421 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
    33481 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
    33541 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
    33601 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
    33661 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
    33721 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
    33781 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
    33841 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
    33901 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
    33961 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
    34021 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
    34081 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
    34141 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
    34201 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
    34261 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
    34321 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
    34381 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
    34441 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
    34501 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
    34561 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
    34621 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
    34681 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
    34741 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
    34801 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
    34861 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
    34921 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
    34981 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
    35041 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
    35101 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
    35161 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
    35221 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
    35281 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
    35341 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
    35401 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
    35461 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
    35521 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
    35581 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
    35641 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
    35701 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
    35761 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
    35821 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
    35881 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
    35941 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
    36001 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
    36061 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
    36121 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
    36181 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
    36241 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
    36301 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
    36361 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
    36421 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
    36481 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
    36541 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
    36601 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
    36661 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
    36721 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
    36781 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
    36841 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
    36901 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
    36961 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
    37021 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
    37081 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
    37141 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
    37201 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
    37261 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
    37321 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
    37381 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
    37441 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
    37501 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
    37561 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
    37621 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
    37681 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
    37741 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
    37801 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
    37861 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
    37921 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
    37981 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
    38041 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
    38101 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
    38161 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
    38221 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
    38281 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
    38341 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
    38401 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
    38461 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
    38521 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
    38581 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
    38641 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
    38701 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
    38761 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
    38821 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
    38881 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
    38941 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
    39001 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
    39061 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
    39121 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
    39181 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
    39241 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg gaagtcgctc
    39301 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
    39361 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
    39421 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
    39481 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
    39541 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
    39601 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
    39661 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
    39721 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
    39781 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
    39841 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
    39901 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
    39961 taaacgg
//
LOCUS       DACCQP010000020        38029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCQP010000020 DACCQP010000000
VERSION     DACCQP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.020"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(400..717)
                     /locus_tag="IVO87_001148"
     CDS             complement(400..717)
                     /locus_tag="IVO87_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7449357.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="IVO87_001149"
     CDS             complement(718..972)
                     /locus_tag="IVO87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7449358.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFRDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1232..1450)
                     /locus_tag="IVO87_001150"
                     /pseudo
     CDS             complement(1232..1450)
                     /locus_tag="IVO87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2010..2096)
                     /locus_tag="IVO87_001151"
                     /pseudo
     CDS             complement(2010..2096)
                     /locus_tag="IVO87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3262..4302
                     /locus_tag="IVO87_001152"
     CDS             3262..4302
                     /locus_tag="IVO87_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7449359.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKLDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKIFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            5128..5460
                     /locus_tag="IVO87_001153"
     CDS             5128..5460
                     /locus_tag="IVO87_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7449360.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            6079..6420
                     /locus_tag="IVO87_001154"
     CDS             6079..6420
                     /locus_tag="IVO87_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7449361.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6439..6729
                     /locus_tag="IVO87_001155"
     CDS             6439..6729
                     /locus_tag="IVO87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449362.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6921..7154
                     /locus_tag="IVO87_001156"
     CDS             6921..7154
                     /locus_tag="IVO87_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449363.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7220..7459
                     /locus_tag="IVO87_001157"
     CDS             7220..7459
                     /locus_tag="IVO87_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449364.1"
                     /translation="MNCKEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            7545..8216
                     /locus_tag="IVO87_001158"
     CDS             7545..8216
                     /locus_tag="IVO87_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7449365.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8269..9918
                     /locus_tag="IVO87_001159"
     CDS             8269..9918
                     /locus_tag="IVO87_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7449366.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDEGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAEKANIKVTNNDGSVVLKENTDYKIENSDYKIENSDSGFVIDFIVNNGQVS
                     DTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTY
                     GAKFLKADGGLFGTGIKATPLEGAEFAAKNAEGKYYSFCGR"
     gene            10427..10828
                     /gene="tnpA"
                     /locus_tag="IVO87_001160"
     CDS             10427..10828
                     /gene="tnpA"
                     /locus_tag="IVO87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7449367.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            10852..11970
                     /gene="tnpB"
                     /locus_tag="IVO87_001161"
     CDS             10852..11970
                     /gene="tnpB"
                     /locus_tag="IVO87_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7449368.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            12036..12401
                     /locus_tag="IVO87_001162"
                     /pseudo
     CDS             12036..12401
                     /locus_tag="IVO87_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            12414..13169
                     /locus_tag="IVO87_001163"
     CDS             12414..13169
                     /locus_tag="IVO87_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7449369.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFILFLFIKKIRRRSE"
     gene            13166..13993
                     /locus_tag="IVO87_001164"
     CDS             13166..13993
                     /locus_tag="IVO87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449370.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            14003..14251
                     /locus_tag="IVO87_001165"
     CDS             14003..14251
                     /locus_tag="IVO87_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449371.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            14282..16381
                     /locus_tag="IVO87_001166"
     CDS             14282..16381
                     /locus_tag="IVO87_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7449372.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IRENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LDNAANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTILVVS
                     LVLLVLVTLSYVALKFN"
     gene            complement(16417..18468)
                     /locus_tag="IVO87_001167"
     CDS             complement(16417..18468)
                     /locus_tag="IVO87_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7449373.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(18588..18797)
                     /locus_tag="IVO87_001168"
     CDS             complement(18588..18797)
                     /locus_tag="IVO87_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449374.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
     gene            18985..19443
                     /locus_tag="IVO87_001169"
     CDS             18985..19443
                     /locus_tag="IVO87_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449375.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFI
                     GKTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            19440..19682
                     /locus_tag="IVO87_001170"
     CDS             19440..19682
                     /locus_tag="IVO87_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449376.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            19700..20002
                     /locus_tag="IVO87_001171"
     CDS             19700..20002
                     /locus_tag="IVO87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449377.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILGAASIITSLFGLFGQTPQGL"
     gene            20072..22126
                     /locus_tag="IVO87_001172"
     CDS             20072..22126
                     /locus_tag="IVO87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449378.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEESDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESIWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NGLYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQRELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            22126..24735
                     /locus_tag="IVO87_001173"
     CDS             22126..24735
                     /locus_tag="IVO87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7449379.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMNMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYKEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            24732..27122
                     /locus_tag="IVO87_001174"
     CDS             24732..27122
                     /locus_tag="IVO87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449380.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDGTPDNGEEQPDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVV
                     TREAAPQPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            27174..27506
                     /locus_tag="IVO87_001175"
     CDS             27174..27506
                     /locus_tag="IVO87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449381.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            27507..28100
                     /locus_tag="IVO87_001176"
     CDS             27507..28100
                     /locus_tag="IVO87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449382.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            28263..30287
                     /locus_tag="IVO87_001177"
     CDS             28263..30287
                     /locus_tag="IVO87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7449383.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYVA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            30287..31492
                     /locus_tag="IVO87_001178"
     CDS             30287..31492
                     /locus_tag="IVO87_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7449384.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGETFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHQGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTGDGTWIDP
                     LPFLNSSKK"
     gene            31508..32152
                     /locus_tag="IVO87_001179"
     CDS             31508..32152
                     /locus_tag="IVO87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449385.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIHQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            32163..32969
                     /locus_tag="IVO87_001180"
     CDS             32163..32969
                     /locus_tag="IVO87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449386.1"
                     /translation="MSKKKGARERFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLH
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            32992..33222
                     /locus_tag="IVO87_001181"
     CDS             32992..33222
                     /locus_tag="IVO87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449387.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVHLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTADNK"
     gene            33381..34316
                     /locus_tag="IVO87_001182"
     CDS             33381..34316
                     /locus_tag="IVO87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP7449388.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            34360..34629
                     /locus_tag="IVO87_001183"
     CDS             34360..34629
                     /locus_tag="IVO87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449389.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            34861..35157
                     /locus_tag="IVO87_001184"
     CDS             34861..35157
                     /locus_tag="IVO87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449390.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            35159..35581
                     /locus_tag="IVO87_001185"
     CDS             35159..35581
                     /locus_tag="IVO87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449391.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            35600..37192
                     /locus_tag="IVO87_001186"
     CDS             35600..37192
                     /locus_tag="IVO87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449392.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            37213..37548
                     /locus_tag="IVO87_001187"
     CDS             37213..37548
                     /locus_tag="IVO87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449393.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            37694..37888
                     /locus_tag="IVO87_001188"
     CDS             37694..37888
                     /locus_tag="IVO87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449394.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT        13753 a         5852 c         7468 g        10956 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgatg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tctcggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgata aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca
     1201 tatgtcggtc ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta
     1261 cacgaataaa gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata
     1321 gcaatactgg aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg
     1381 catacttaaa tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa
     1441 atggatcaat agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac
     1501 gttaaaaaat attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca
     1561 aaactgatcg atattggatc caaaaatata tactacctaa aataggtagt atatattaaa
     1621 atatgtgctt ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag
     1681 gtatttcagt tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg
     1741 gaggaggaga tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag
     1801 aggtaactgc acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta
     1861 agcctacccg ttaattctat aatatagaaa cgtaaaaaaa accccatcag cctgcacgct
     1921 gatggggttt ggtgatataa tgaaatacct ctttgtaact cataattata ccataattag
     1981 tcacgaatgc aactaataga aaaaactact tagaacggta gatcatcgct aataacaatg
     2041 cattgtccac caaatggatt ttgtgaagca ttaaatccat tgttattaat tgtcgctgct
     2101 tttaactagg cctctcccct tctattataa aagaagatat atatcatttc ttaattacat
     2161 actaccttaa accaaaattt aagtactacc taatttaagt agtacttaaa taatctaaaa
     2221 aatagttatc ccaattgtta tgcttttata atacgattat ttgtaacaaa atgaatatta
     2281 tataattaaa cactaccata tttaggtagt gtttaattat tagcaaaaca aaaaatattt
     2341 attctattct cttaacttta actaagttat gctgcacatc tttatataaa tcatacgcaa
     2401 ctcattagct agacaaacta caagcatgta gttttatctg ttaattatgt actacctaaa
     2461 taaagtagtt tataattaac aagtatatat atttaaaaat ttaaaattaa tttttttatg
     2521 acttgtacaa atttatctga tattctctta ttaagaacta cctaatttgg gtagttctta
     2581 ttttttacat tttaagtagt atttaattgc aatttggtag tattttttta ttatcttatt
     2641 cctagaacgg tagtacttaa tataaatata ggtagtatct aatttaagta ttaatggtag
     2701 tatataattt aacaggtagt atgtaactac tttaaagtaa tttttttcag attgacacag
     2761 tagtatctta ttaaataagt taattttaaa ggtagtatat aattattata tatatctaac
     2821 attggtagta cttattgtat tttttagtag tatataatct attgttttat agcctaaagc
     2881 cttgtctcat aagaataata taatatatac tactgaaata aggtagtata tattactagt
     2941 aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg
     3001 gtagtactta attatcctta attttaaaac taaattttat tcatacgttc ttctggtagt
     3061 atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag gtagtacata
     3121 attcatttta tttcttattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc
     3181 attttttatt tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa
     3241 cgtgaaagta gaggatctac aatgactgat tttaattttt ttaaggcgga tagagtttac
     3301 aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg
     3361 aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc
     3421 aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt
     3481 agcgtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt
     3541 ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat
     3601 agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt
     3661 gatagagaga atagagaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc
     3721 gacgaaaaat tagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc
     3781 caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta
     3841 gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta
     3901 gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta
     3961 ttaaatttga taaaaatatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact
     4021 attcataatg caaagaaaaa agctgaaata gaaagtggta tttcgatagt ttttgaagaa
     4081 ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat
     4141 caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa
     4201 aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat
     4261 gaaagctccc caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg
     4321 attgttgctt catgaagtaa cattaaaatt aaaaattttt tattattcta attatattac
     4381 gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat tatgataaaa
     4441 atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag
     4501 aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag
     4561 ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga
     4621 atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg
     4681 attttcgttc tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaaaaga
     4741 aaggcggtat gaactcggaa ggctggtaac cttcgtcagc gttcatacac ggcattaaga
     4801 tttggccctt aatgcgttaa cctttcagtg ttacggtatt acccgcaatg gatttagtat
     4861 aacagatatt caaaagtgat accacttttt ttggctgtgt taaggttaaa aatacgtgat
     4921 attacgatga atctatgcct aagcataatg ttataaaaat gtccgtgtgg ctctttgccg
     4981 tatgacgaac gctgaaatta gagggaacgc atgtttggat gcgttccctc ttttttgttt
     5041 gtcatgaacg aaaatcaatt ctctattttt gaaaaataac aagaaaaact tgttaaaaat
     5101 atttaaagaa attgggtggt ttattttatg tcatatacag gaattttgag tctcaaagat
     5161 atttgtcact acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa actatcaact
     5221 ggccaaagta aaaccgttgt gcagtgtaag aaatatataa tccagaaaga caaagtctca
     5281 gaggaaatga tttattacat tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc
     5341 ttaaaagagc tatatccaac aatcaaacat gtttatgacc aaaacggtgt actgattggt
     5401 cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga tggggcgttt gatcggctga
     5461 attgatcatt ctcataactt gtattttatc gtttttcgct tgcttgaaaa tattgtcgag
     5521 acttctaaag gaacttagag tcactagaaa aataaggagt agacttgatc tatcaacaat
     5581 tcccttcggg gagtgacggg gtaggtttta acgattggta acgtaaaaaa ttggtcaatc
     5641 gagtaaatgc gcttgtttgg atagtggtga aatgaatttg tgtaaataat atacacagag
     5701 taaacaagag ggaatttaga agttagacaa gttagaatag cgtctagatt aatcatgagc
     5761 agctactaag ggatctagac tttgacgcaa actcatggaa taaggagtag gtcttctcgt
     5821 aatggggagg acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag
     5881 gacgttgatt attgaatcaa tcgagcattt accgctgtct actagggttt ctcgtttcgg
     5941 acgccataaa cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa cgataaaata
     6001 tgaacaagtc ctgaataaag tgaaaaaatt gatataaaac atagagaaaa tgaaatttac
     6061 gtaatggagg aatgacaaat gaaaaattat gatccgaata tacgattggg cacacatact
     6121 attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag aaggggcgga
     6181 aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga tcaaacattg
     6241 actgataatc caattggatt cggcttgcta cctgaagatg atgaaggtaa tgaatggttt
     6301 aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac ttggtcatat
     6361 ttgagtgatt atattgtcag cgttgaaatc attgaatttg tagcggataa agaagaataa
     6421 aaactggagg aataaattat gaaatttttc gaagtaaaat caccatacta ttatgcgtta
     6481 atcgcagcaa agaatgaaga agaatgtgca gaattgtatg aacaagtagt aacagatatc
     6541 gaaaatcgtg aagaatttat tcgatgtttg catgagttaa atcgaaatga agcaattcaa
     6601 gaggatgcga aaaaaattag tgaagaaacg ttacaaccaa tcggtatgga ggaagctaca
     6661 aaagacattt ttacaattat tgatgaacaa aaatcttgtg tattaagcat tgattctaca
     6721 ttggtttaat agatccaaga ttctataatt tttttcaaag taaaaagaaa ttaggtaatg
     6781 attctcagac caattactta ggaaaataac catacaaatt agattttaca gataaaggat
     6841 tagaaggaat cacggcgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     6901 ttatttagga ggaaaattag atggcgcgac cagttttaga tgcgatggat atggatactt
     6961 tgacaaaagg aaggtatgta cagctgttag ctcgtggtta ttcacctaaa gatgtattca
     7021 aagccctatc taaaggaaca gatatggaaa aagaaagatt acgaaaagaa ttcgactatt
     7081 ggagagagca taacggaata aaagatttaa aaccagcacg tccagttatt agacgaaaaa
     7141 aattgaagaa gtaagaccaa agcgtatggc gtacataggc ttttttattt tgataaaaat
     7201 aatgaaagga aggaaaacca tgaattgtaa agaaatcgaa aatagaaaaa aagtgtcgaa
     7261 agaaatggaa gaaaagttac taaaaacaat gaaacaaaaa caccttaaaa gattatctgt
     7321 cgcgcaatat atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc
     7381 aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa aaaagttcta attgtcaact
     7441 attgttagaa gtctcatgac attcattaat agaattcatt tttaaaactt tttctatgga
     7501 aatgagaaaa agtttttttg ttttgtctga aaggaggcag atggatgaaa aaatggttat
     7561 ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt gtttggctat gttagctacc
     7621 aaaaacatga aggagatgtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa
     7681 tcaatgcaca cgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt
     7741 tggggcaaat gttgtccata caaaaagaag caatcgaaat gggcgtcaat aaacaagtat
     7801 ttgcacaaat tcagattcct gcacttggtt tagcattgcc gatttttaaa ggggcaaatc
     7861 aatacacttt gagtcttgga gcggctactt atttttatga ggatgccgaa atgggcaaag
     7921 gaaactatgt tctagcagga cacaacatgg aaatgccagg tgttttattt tcagatattc
     7981 aaaagttgtc attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca
     8041 aggtaacgtg taaatttatc gtatctgagt actttaaatt aattgatggc gttcctgaag
     8101 agaactcttt cttgtcttta ccaaagaaag gagagaaacc cttattaacg ttgtttactt
     8161 gtgtttatac gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag
     8221 gttgtaatca acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat
     8281 cggtatgtaa aaaaagctgg aatgcttgtt ggaatgttag gaatcattgc aggctgcttt
     8341 cagggatatc gagcatatgc agaagatgtc acgcaaaaaa cgccaccaga gaaggtcaat
     8401 atcacggttc ataaattaat gtacgatcaa ggaacgcaat tgaatgttga tattgacggg
     8461 atcaaaaatg atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag
     8521 gcagattatg gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgagg
     8581 gaagattctg atctaaccaa cgcgaaagtg gacgaaatcg ttaaagatgt agaagacgag
     8641 ggatctaaca gtgagtacgt taagaatgca accaataaaa ttacttcagc tgtagatgaa
     8701 aatggtgaga tcacttttgc tgatcaacca gcctatgtca attccaaagg gaatgtatat
     8761 gtggtttatg aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt
     8821 gtgattgcgc caatgactga taatacaggt tcaggttttt taaaagacat tcacttatat
     8881 cctaaaaata ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcaca
     8941 gctcaaagca aacaaacacc tttaaaaggg gcaaaatttg agttatacaa gggcgaacct
     9001 ggaaaaggaa caaaattagg agatcttgta tcggatgatc aaggaaagct gactgctact
     9061 gatttgacgt taggaaaata ctattttgtg gaagttcctt cagaagttgt agtgggatca
     9121 gataaagaac caacagcaga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac
     9181 aataaattaa cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac
     9241 gtaaactata aagcaccagt gatcgataaa acagtaacaa atggaacagg acaagaacat
     9301 tctttccaaa ttggtgacgc agtaaattat caaggaacga ttcatatccc aacagacatt
     9361 gcaggcggag cagacggtat cacagtaaac ggagtgaaaa gcgaaacttc tccttacagt
     9421 gtatttaaat ggggggatac agcaggacaa ggactttctt atgtcgctga aaaagcaaat
     9481 atcaaagtca caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc
     9541 gaaaattctg actataaaat cgaaaattct gatagtggtt ttgtgatcga ttttatcgta
     9601 aataatggac aagtaagtga tacagttgcg aatttacatg gtcaagatct aaaaatgact
     9661 tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc cattaacaaa tagcgtggat
     9721 ttcgtctata gcaacaatcc attcaatcag gaagaacacc atgaaaaaac gaaagccgac
     9781 gctgtaactt atggcgcaaa attcttaaaa gctgatggcg gactatttgg aacaggaatc
     9841 aaagcaacac cgcttgaagg cgcagaattt gctgctaaaa acgcagaagg aaaatattat
     9901 agcttttgcg ggcgataaaa cccacggttt taaccgtgtg gatgatagcc cgcactgtac
     9961 tgtaagagca tagggaaact tgtgcttagt ggaatatata aaatctcgta tatcccaaca
    10021 aagaaactga attgcgggga acccctaaag cgagtaaaac gacaacgtag tcttgaaata
    10081 ggcaaacgtg accgtggcga aagcagaaaa aatttactcg atagcatatg gttaaatcct
    10141 aagtgttgcg acaatgggca atctgcagcg aagactcgaa cagagtaacg ttcaacgact
    10201 attcctcttg agggaagtac acacaagcgt gtggaagtgg tttcgccgta ctgggtaaag
    10261 ccaacggata agatatagtc tgtgctggca cgaaagtgtc agaagttcat cagagaactg
    10321 cataggaagt agcgaaccta tgtgaacaaa ctccacataa aaaattcttc attcttttgt
    10381 gtatattaac attgatttag tacatggctt aacctaaaat taaggtatgg ataacgaata
    10441 cagatactca aagacaactg tatcgttagt caattaccac tttgtttttt gcccaaggta
    10501 tcgtcggaaa atattcgtta attctgaagt agatgctaga tttaaagcac tcgttcaaga
    10561 aatagcaaag gaaaacgagt ggaacatcat tgcaatagaa acagataaag atcattgtca
    10621 tttattaata aatgttcagc ctacgtattc tccttctaaa atcatgaaga taataaaagg
    10681 taagacatct agcataatcc ggcaagagtt tgaacactta agtaaaatgc caagtgtttg
    10741 gacacgttca tactttgttt caaccgcagg aaatgtatct agcgaaatta tcaagcgata
    10801 tgttgagaac caaagaacaa gatattgatt gcgttaagga ggtgtgcaga aatgaaaaag
    10861 gctcataaaa tcaggattta ccccaactgt cggcaagaaa ttcaatttca aaaaacattc
    10921 ggatgcgttc gtttctattg gaactttcta ctagatcaac gaataacgaa ttacaagaag
    10981 aaaaaagaaa atgccgatta caaagaagat aagacaacgt atgcttcatt gaaaaaaatg
    11041 gaagaatacg cttggctaaa agaagttgaa gctcaaccac ttagccaagt agccatggat
    11101 ctaaataaag catttaaaaa tacgttcaac tctacttttg gatttcctaa attcaaaagt
    11161 aagaagtatt ccaaaaagtc ctatcgaaca gctatgggta taaaggtgaa tagaaactac
    11221 ttttatgtat ccaaggttgg ctgggtaaaa atggctgaaa cgttgcgttt caatggtaaa
    11281 ttaatgaatg tgacaatcag ccagtcaaaa acagaaaaat attttgcaac ttttttagtc
    11341 gatattgaaa acttccaaaa agatcctgtg acggaatcca ttggtttaga tttgggtctt
    11401 actcatttct gcataacttc cgcaggtgag aagataggaa acgagagatt ctatcgatca
    11461 ttagaaaaac gcttagtggt agagcaacgt aaactttcaa gacgcttaga agtagcaaag
    11521 aagcataatc gtaaattaga agagtgcaaa aactaccaaa agcaaagagt gaaagtggca
    11581 cgtatccatg aaaagatttt taaccaacga aatgactttt tacacaaact gtcttctcgt
    11641 ataactgatg agaaccaaat catttgtatt gaggacttga atgtaacagg catggttaga
    11701 aatcataaat tagctaaatc aatagcagac gtatcttgga gtgaatttgt aaggcaatta
    11761 acatacaagt gtgaatggaa aggtcgaacg ttagtaaaaa tcgaccgttt ttaccctagc
    11821 tcgcaaatat gttcttcttg tggtcataat gacggtaaga aagagttaga tattagagaa
    11881 tggacttgta gcaattgcgg aacaaatcat gacagagata tcaacgctag tataaacatt
    11941 agaaccgaag gactttcggg gatagcttag taaatttgcg cccgtttggg tgtattacct
    12001 aagaacccca tgactttagt cgtgtggagt gtcagggtgg attagttgat acggataaag
    12061 acggtgtcaa agaagcagta tgggtagaca atgtagcaaa tgcagcgatc ctaaaatcag
    12121 ataaagaagg gcgctttgaa atcactggtt taactgaagg tgaatattca ttggaagaaa
    12181 caaaagcacc aactggttac caaaaattaa cagaagatat tgcatttaaa gtaaacaaaa
    12241 actcatttaa agaggaaaat cgaatcactg ttaaaaataa tcaaaaagca gctattccat
    12301 taacaggtag taatggtttc caaacgtatg tattgatctc ttgcttgttg ctaggagcag
    12361 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
    12421 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
    12481 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagctaaa tttcaacaaa
    12541 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
    12601 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
    12661 caatttatga cggaacttcc gaaaaaattt tagagaatgg tgtagggatt acggaaggaa
    12721 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcagggcac agtggacttt
    12781 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
    12841 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
    12901 aattacaaaa gaattattta gattatctca tgcctgaaaa agaccaagat aggattacgt
    12961 taatgacctg cacgccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
    13021 tttcattcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
    13081 cattggattg gcgattagca ctgctattcg ctttttttgc gttcatatta tttttattta
    13141 taaaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
    13201 agctctcttt gcaactgcag cttactcaca gatatctttt gctgatagca gtaccactcc
    13261 aactaccagt cagacaacag atagttcaac aactgcaaca agcgacacgg acagttcgaa
    13321 gaacactgca gaagaaacgg cagattcaag tacgagttct actgaatcag cggaatcaac
    13381 gtcagaaagt tcttcagaga cttcagaaaa actgcaagaa gtagaagata cgcaagtaat
    13441 caagcaaaac attcatattc aaaaaattat ttcttcgaat cgaatcaaca atcaaggaag
    13501 gcaagtagct catcaagaag gtgtcaatgg cgcaaagttt attgtctacg atatcacgaa
    13561 tatcttagaa gaaatgacac aagatgataa tgagaaaaat caatcggtag aaataattga
    13621 gtcaaaattg aaagatcgag caaaaaaact ttcatacgac cagttgaaaa aagtgactga
    13681 aggagaaacc aaaacaattg atgatcagga aggtgtgatt gatttttcaa ttgaagtacc
    13741 tgcaaacaaa aaacaagcat actatatcgt caatgaatca agccctgaaa acatttcaaa
    13801 tagcgaagat attattttat tgacacctat ttcagatgaa aagggtgaat ttttgacgga
    13861 tgtttggtta tatcctaaat caaagaaatc agagccgaaa gaagaaatta aaaaagttgt
    13921 atcaactggt gtaaagaaaa acttttttga taattgttgg gattttttca ctcatttatt
    13981 ttttaacgat taggatgaaa tgatgaaaat aataaaaatg ttagctttta atctatcttt
    14041 tttagccgca acaatattgt gttgcggttt agttttttct caattatcaa tttatccaag
    14101 atatcaacga ttgttcgttc attttctaaa tatccagcat gcttccatgc cactagaagt
    14161 gattaacatg ctcttaatca atccaatttt tatggctagt ttgtttcttc ttgcaggatt
    14221 ggtaacgtgg gtattaggaa aaaaagaatg agaagcttac aatcgtttaa caaaataata
    14281 gatggagggc attatggaaa ataaaattca ttgcactacg ggaagagtat ttactttagt
    14341 gttgtttttg ttccttcttc tttttgcaag gggaactgca gtccaggcct tagaaaaagt
    14401 tgatccgagt caaatcagag aaaataataa tctggtactt tatcatgata gtgattcatt
    14461 gagaaatatc gaagtaactg ggatcaatgg atttaagata ctagatgagt attcaactaa
    14521 aaaacaaaaa gtacttgctt ggccaaaagt caaagatttg aaaagcacgt caaaaattgt
    14581 tgcccattat caaaaggtgg gcacttatct aggaaaagaa attgattgcg acgtaacttt
    14641 ttctaatttt gtagcgcacg ctggggttaa ccaagacgga ttagctaaaa caattaatgg
    14701 gaaaaagtat tataattatt tgctctttga taaaaacatt tatcgaggct ttttcatgca
    14761 gtcaatcaac gttatgaatg taaaactaga ttttaagtat tcaggatcaa aaacaaaagt
    14821 agctttgagt gaaccacagc cagaaattaa ttcaaaaatg agatcgatga gagcagctgg
    14881 tattgagttg aatgatacat ttctatctat ttacagttta aatggacagt atcagcaacg
    14941 tgtgtcttgg tataatgaca ataatgaatc tgtcggtgaa cgtaatattg atatgagaga
    15001 atttgttggg tatgaaaaaa tgggtagctt accttatttt gtcacaacag atacagcatg
    15061 tgcagaatac aaagctcctg cgttatcaac aaacaattta acttcaaaag tagtgggagg
    15121 acgtgcagaa aaggcttata gctcgaatga tcatttcacc gatgttgtag gagctgatac
    15181 ttatcacaga agtggtgtaa cgtatacgct tcaaggcgct tccccaacat tcatgattgg
    15241 cgcaaatacg aatagtatga tgtttagctt tgatactgca ttgctatgga caccacaacc
    15301 atcgaagcct acaaaagaag tgtttaacaa agctaatact gaagaggcag cacacaatat
    15361 tgacaaaaaa gtgattccac aaggatcaga tgtttactat catattcacc aaaagtttga
    15421 tgcattaaca gtcaacacaa tgaacaaata caaatcattt aaaatcactg atacctttga
    15481 cagcaaaaat tttgatatgg tatcggatgg gaaaaactat gatggcgcat tgtggtatgt
    15541 tgaagcgaat gggacttgga aaaaagctga tacaggagga aaattaactg tatcaggtaa
    15601 tacagtcact tatactgctt ctgacagctt tttagataac gctgcaaatt acggtaggga
    15661 ttatattttg cagctccata tgaaagcaaa aaatgatgca aaaggaattg caccaaatgt
    15721 ggctactgta acgtttaaca atgatccgaa caaaggaata caaaaaacaa atacagtaga
    15781 aaactatttc tctcttaaac caaataaaaa agtggaacaa gatgggaaag atgttgatgg
    15841 tcgaaatgat ggtaaaaaag gaacgcctac agctccttta aatgcaggat ctgaagtaca
    15901 atatctagtc acacaaaaat ggcatacaaa aggtgtagac attgcgtcag atcattacaa
    15961 gcaagatcca gtggaagctc gtttgacgta taaagaaggt tctgcgcgag tgatagatat
    16021 gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca atttaagaac
    16081 gctgaaatgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg gacgagaaat
    16141 tcaactgata tttacagcta aaactccact acagtcagaa aaaaatattg ataaccaagc
    16201 cgtagcagca gtagagaatg tttctaataa aacgaatgtt gtaacaattg gtgttgatcc
    16261 taacttgcca caagtcattg ttcctagaac aggttctaca catttagtaa caattttagt
    16321 ggttagctta gtattacttg tgttagttac tcttagttat gtggctctga agttcaatta
    16381 aacaaaaaaa caaggctgaa aatcagcctt gtttttctat ttgaaaattg gattggcact
    16441 aataaatacg ttgtctgaag aaagtgatcc atctgcagaa acttcataag catgagataa
    16501 cccaacgatg ctttgattgt tgtaaagtaa gtttgcgtta tgagcagcgc tatctttcca
    16561 cataatcatg gcattcactg ctcccgtatc tgaaaggatc tcgtcaacgt aaaggtagaa
    16621 actattgata ttttctcctt tagcgccgta gcagcctaaa ctttctaaga gatcttggaa
    16681 agtaccgccg ccaggtcgat aatgactgaa gcttacttgt aattcttttg cacgtatatc
    16741 agcagcttgt tgaacgatat cattaaattg cattggagct gcaccgtttt gttgacgata
    16801 attatttaac catacaagtt ctttttgacg cacatagttg tttaaatcac ttaatgagat
    16861 atttttgtat ttgaacgtga tcgttcgatt ttggtcaaca ttattgatta ctcgttggcc
    16921 gtttttatct acgctaatgg aaggatcgtc atttctacac aagaaataat tttgatcagt
    16981 catatttttc caaggagtag agaagctatc cccttcgtat actgtttttg attcactaga
    17041 gagaacagcg ttggtatcag catttacatg attaacagtc acattaaatt tacgagtgta
    17101 gttgaaaata taattttgac tttcagtcac attgtttaag gttactgaat ttgttggttc
    17161 tacccgatcg tctaaaacat atccttgaat agcaggagca gttaaagtga cactttcacc
    17221 gtgagtgacg ttgattatct gttctttcaa aatatcaact cgcccctcat ttttgtagtt
    17281 gttattgcac aacaaattga tttgatgcac ttgattataa ttgaaggtga ttgttgtatt
    17341 acctgtgatc gtttggcttt gagtatcatt accaatcact ttgtagctaa aattatcttt
    17401 agcgccttcg ctatatttaa atgcttttgc cttagcggtg taagtagaac cagaagtcac
    17461 ttgtgcttga tctgtagcaa tcacttgacc tgtttgattg tcaatatggt taatggtaac
    17521 catatatggc gtttcaggtt tgtgttgcaa ccctttaaat gcagcatcta gactattttt
    17581 agcattattt acttgggctt gagaagcctt agaatcgtct agtacttttt tagcattgga
    17641 taaagcagtc tgaaaatcat tccaagattc ttctgtaaaa tctcctttag cagttgattt
    17701 tactcggttg tataaagctt gtaattcagc tttattcaca gtaggtgctg gttttttctt
    17761 taatcccttg taagcagaat ctaaattatt tttagcacta tccacttgtt tttgagtcgc
    17821 ttttgagtca tttaacactt tattagcatt atttaaagca gtttgaaatg cgttccaggt
    17881 attgtcggta tagtcacctt ttttcgttcc cttaacgctc gtataaagag cttctaaagc
    17941 tgatttattc actttgggtt tagaggtgtc agcgccatac caaccgatac cttcgtattt
    18001 ccagccatag tctttggtta accattgtac ttctttccaa cttgcagtgt agtgatgtga
    18061 gccagatttt acaccaggaa ggaaaaggcg gtagatcggt ttattatttt tttcggcaga
    18121 acgccaggca ggcccttcat aagtccagcc gtaattttta gtcaaccatt tcacttcatc
    18181 ccaactagct gtgtaatggt gatcacgaag acctttattg tataaacgat agacaagatg
    18241 accaccgcta gtcggtgctt cccaagcggg gccttcataa ttcccccact taatcttcac
    18301 taggtgatca cgttcttttg tgctagaggt gtaaaaatgt tcatggttat ttggattata
    18361 aaggcgatac atgacgttag ttgaagcagc atcagcagat ccagcgaaac caagtgtggc
    18421 agcaaatgtt ccaatagcta aaacagtttt tgggtttaat aatttcatat tttcaactcc
    18481 taaaaattag ttaaaatcga ttacaaaatc attatatatc ggtaaaccga tatggtcaat
    18541 aaaaaaatta agaaaagcct catgtatttc tggcttttct taatttgtta cttagttgaa
    18601 gcgttagtat caatggtttc ttcagctaat ttatttaatt ttatagcatt gatgaatctc
    18661 atattgtcta tactagagcg cttatttgcg agatcactga ccgtagttaa aggtacgcca
    18721 gattctatag atatagaacg aactgattta ccactatcaa acagcaacca ttcgattttc
    18781 tttttatcag cgtacattgc aatgctccct cttgattttt tctttattat agctcttttt
    18841 tgtaataaat cacaatatca agagagaaag tttttataga aaggtgggaa attatgagta
    18901 tccgttcccc gtgtatcaaa atgaggtttg ttgctacttt cgttatttga tatactaaaa
    18961 taaaacaacg aaagaagaaa taaaatgaag aaaaagcaac tatggtttgt ctctatgttt
    19021 gcaatatttt ttattatttt aagtggatgt tcaagtaata agaaatcgga agctaaagaa
    19081 gataatagtt tattgcaacc aggaactgtt tggaaagaag aagtaggtgg tcttgttaat
    19141 aatttgaaaa ttatagacga tacaacatgg gaatattccg aaagtgtttg gcatcctgaa
    19201 caagtacaaa taacagttga gaagctaaaa gactataaag gattagaaag atataaggta
    19261 atcgattcag gtggaataga tttattcata ggtaaaacag ccttaatagt tccctataac
    19321 aaagacggtt tagaaacgtt taaatttatt ccaacaaacg aaaaagaaca tcgagataaa
    19381 agtgaaatat taaaagaata cagtactaaa tctggataca agttacaaaa gactagcgag
    19441 tgattgctag tctttttatt tatcctgaag gaggaatcac agtggcaaaa gctaaaacgc
    19501 catttgaaaa agccgcgtct aaaatcttag aacgtcgcgg tgtatctgaa acacagtgga
    19561 aacaagaagt ccttgaaaaa gcacaatttg acctattacg gggaaaagac aaggacttag
    19621 aaggatatat ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt attaaacact
    19681 aaaaggagcg aacaatttta tgtttgaatc aattacaatg aattttttaa cacaaccgaa
    19741 agtagtagct gcggttgata tttttagagc attacaaact gcagcaacta gtttaaaaaa
    19801 caatttgatg atttttgctg gtgttgcggc cgttgcttcc attgttattg gggggctttt
    19861 cttcctattt ggacgaagag gagctgaaaa cgggaagatg cacgtaggtt atgtattgat
    19921 gggggtaggt gttattctag gggctgcttc tatcataact tcgttatttg gattatttgg
    19981 gcaaacacct caaggattat aagaaagtaa aaaagtagga gtaagaagta gtcgatactt
    20041 tttactcctt ttttatgaaa ggagaatatt catgtacttt acgattgcag gcaataccaa
    20101 aacagataaa gagttaatta aaaaagcaac gaaggaagta ggaaagcgga cttctttttt
    20161 accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg tgttagggaa
    20221 agtactggaa cacgaaagtc gatcttctgt taagttgact ctcacgttac atgacacaga
    20281 aggaaaaata gagaaagctg gtacagaata cgtttttggc ccgctggatt atcaaatttc
    20341 agaaggtttc tatcctacat tgatggattt aatccgcctt gatgtgttta aagaaacgga
    20401 tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa cttttacaag ttttagaaga
    20461 agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt gaagtcgaag taaatgaaga
    20521 agtaccatgg gaacgaccaa tctttccaaa gaaggagtca aaagaaaaag agacagtcta
    20581 tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag agagcgatgt
    20641 gatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag agacggccga
    20701 agtagaagtt gatccagttg aagctaatga agagtcaata tggactgaag gggattctca
    20761 ggaatatatt gaaccactta tcagtgaaga agtagagaaa aacgaaacag agatacaaaa
    20821 ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat ggatgaaaga
    20881 aaaaaacaaa ttatttttaa atgggcttta ttcgaaatat caattacccc aaacctatca
    20941 agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac ttttagaaca
    21001 gttagagtct gtccaatcaa caaattggca tgagcttgca caagaagatt tattagccac
    21061 ctttgaagaa cgtcaacaag aaacagatca agaaattaat gagtatgcga cggaacagtc
    21121 gtctaattgg gagaaagcaa aggaggaact aaataaagaa gaacagcgag tgattgaaga
    21181 agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt attccttgtg
    21241 tgtagaaaag atcaataatt ttactcagac gaatcaagaa gcgttatcga aagaaaaaga
    21301 aaacttgtta aaagaacgta taagagaacg aaaagaagaa ttttatcaag gtttgcgtac
    21361 agataagcta aagattagca aagaattaaa tgagcaactg aataagctat atcaaacaac
    21421 gactgaagtt cttatggaaa aacacgagca gatccagcaa gaattacaag tacaaattcc
    21481 aaaatggaaa aaagagtatg aaattcaaca acgagaattg aaagaacaag aagaaaaaca
    21541 acggcaaaaa gaaaaagagc gcgaacaatt agaattaaaa aaacgtgaac aggatcttgc
    21601 ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa gaaaaggaaa aagaagcagc
    21661 tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagcacagt taaattatct
    21721 agcacaacct caaccagtag ctgctgttca aacgccagtg aacaaccaag caaatttaac
    21781 tccttcacca cagcgttcct ttaacggatg gatggtggga tgtattgcaa gtttaagctt
    21841 gcttctaggt ggaggaacag tatttgcttt taatcatatc aatcaatctt cacaggctga
    21901 ttctgtggcc acaacgacgt caatcaagga agaagcccaa gaacaagcaa gaaaagagta
    21961 tgaacaacaa ttagaagaat tattgaatcg taccaatcag tcaacaacag agtcaagtat
    22021 ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa ggatcaagcg attcaactgt
    22081 acaatcaaac acttccgaaa gttcagaatc aaatgaggtg aaataatgcg tgttagagaa
    22141 acaacgagac gaaaagacaa acgattcaat ttagaaaaga ataggtatct ttctcatttc
    22201 tatcgctata ctccttcagg agaagagcta ccagaaaaac atgacaaacg tatttctcaa
    22261 aagatttttt atagtggctt agcttatctt cctttaaact atgttgttac aagaatacca
    22321 gaagcatttc atcgatttga gattattcca ggaagtttac aaggagtgaa tatttttcag
    22381 ttacaagaaa tgccacagtt attccatttt gattcaactg gtttttggaa tcctttgacc
    22441 ccaacaacaa gtttaggcac acgtttagtt gtttctacga tttgtgttgt tccagctgta
    22501 cttatgggat tgaaaatgaa catgtggttt cgtcctctag cagatgggca aaaaggagat
    22561 aaccgattaa aaacgttaaa ggaaatccag caaatttatc cgcaaatacc agaatcaaag
    22621 ggaggttttc ctggttatgg cggaatacca attactcatt ataaaaagta ctggtatatt
    22681 cagacggata cagtaaatac ttgtattgtt ggtacttccc gttcagggaa aggacagata
    22741 gaagttttag caaccattga caatttgtct cgtgcagaaa gacaaagttc aatggttgtt
    22801 aatgatccaa aaacagaatt atatgttgct agtaaagatg aactagaagc tagagggtat
    22861 gatgtgtatg catttaatat caccgatcca ctgcaatcaa tgagtgataa tccattggca
    22921 ctcatcgtga aatactggaa gcgaggagac atagacacag cgacacagtt aaccaatact
    22981 ttcacgaata cgatttatta tgatgccaac gcttcagaca ataaatactt taacgaaaat
    23041 gcgcaaaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga tcagacaggg
    23101 gattactcca aattaacacc gaataatctc gtcgaacttc tttctgagat tggtggtttt
    23161 aattaccaag atcctgataa tgaattcaag cagctcaatg ctttagatga atttatgaat
    23221 cagttaccac ctggaaacat agcaaaaaaa caatatgggg caacaaaaat tggttctgat
    23281 aaagcgaaag gcaacattct atcgacagca atcacaggac taaatccatt tacactgacg
    23341 aaaattgcga aaatgacttc tcaatcaacc attgattata aatccattgg ttttccgaag
    23401 tatttggatc tcaaactaca cgagagtctg ttaaaccagc gaattgttgt agagttttat
    23461 catcaaggaa aaaaatttca tgaggaacgt gtcaaagtag gctatagagg tttttgtgaa
    23521 attaactttg attgtcattt agaacaggga gatcatgttt gcttaaaata tgatcatcga
    23581 ggaaaagaat atgttgcttg gtatgagttt tctcaaagag tattgaaaga cgaagaggga
    23641 aatatcgttt atcgaaaaaa acgaggagaa acccatttgc cagaattaga aaaagaagcc
    23701 gttctaacat tagatgagga taaaagtaac ttattagtag agtctattcg gatgcactat
    23761 accgataaac caatggctat ttttatgctc acacctgatt atgatccatc taaccatatc
    23821 attgttagta tttttcttag tcagttgtat aaagaattat ctacgcaatg tgttaagact
    23881 aaaggagata aatgtcaccg taggattcat tttattttag atgaatttgg gaatatgcca
    23941 ccaatggata atatggaagg aatcatgaca gtcacagctg gtagaaacat gttgtgggat
    24001 ttgtttatcc aatcctatca gcaactttat gcgaagtatg gggaacaaaa cgcagcgatt
    24061 atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa taccatcgaa
    24121 gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac catcaattca
    24181 ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt aactcctgaa
    24241 agaatttcta cttttttaga aggtgaaacg attgtactcg ctcctttaaa acgacaagat
    24301 ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac gaatcttcct
    24361 tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga tatagacata
    24421 cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattacga ttattttcta
    24481 cctgatatct atgcacgtcg actttatcac aatatgcacg atcaaataga agaggaagct
    24541 aaagaaaaca tttttattaa aatcgttcaa tctttagaca actcaacggc acaaaagtta
    24601 cttattcaat tttataaaga gaagcaaata gatcgtttgc aagaatacct tgaaaaattg
    24661 cgtcaaaatg aagaaattac agaggttata aggaaacgac ttttgctaga agctgaaaag
    24721 gcggtgaaag aatgatcatt ggaggaatat tttccggcat tgccgactgg tggtcaggct
    24781 tatttggaga tggggatgca gatcccaaaa ccgtaacgaa agtcttacaa gattatggta
    24841 attatctgca gtatcaagat gtattcagtt ggatttgtaa tactttttta tggggtttga
    24901 ttaaagggtt atataatctc aatactttac tagaaaaaac aatttatcaa agctttgatt
    24961 taaaagactt attgaacgcc gctggtgtta atgaactgtt tcaaagtttg attagtaagg
    25021 tattggctct tttttgtgtc gtcacattaa tttatttagg cttaaaattt tttatgagta
    25081 aacatccacc aaaaatcaaa aatatcttgg ttaatctatt tatggccatg attttgattt
    25141 ttggtggtag ttctttaatc gatgaagggc taaatattag taaaaacttt tacggagacg
    25201 tgactgcagc aaataaagca gataaaactg gttcacctgc ttttcaaatt attcaaaata
    25261 atgtgattga tgtgagtacg ttactagaaa atgatccgaa caaagtagaa cacataccaa
    25321 cagataaaag aaatgctctc acggcgaaaa attttaaaac agcgaatatc aatgggatta
    25381 ttacacctga tcaagctaag aaaatgtctg aatcaagtga tattagtaca gtacaaaaag
    25441 atcggttaaa ggcattacag tatcgactag agttagacga taatgggaaa gaaattcctg
    25501 tgcaaatcag tgatgaaggc ttagccaaat atgtttatac ttcagggtat cgtcgataca
    25561 ttagtagtcc agggattatt ttagctggtg aatcttcttt ggcggtggcc tatttattta
    25621 tcttgtttac gattgttact tgtattattg agctggtatt taaaaaattc tatttagtga
    25681 ttgccgcgtc aacggacttt gagacaggac aacggatgaa aagtgctata gaagatattt
    25741 ctcaatcctt cttgttattg gcatttacaa ttttagaatt acgaatctat acattaatgc
    25801 tttcaggtat tggtgatttg catgcggcag gaaagatcaa tggattcttg tatgttgtcg
    25861 ctttgattgt actaaccatt gcgctattca aaggctctca atcagttacg aaaattttcg
    25921 gcgttgatac gagtctgaaa aacggcagca attctcttct tagcatgttt gccttaggaa
    25981 atattgcgaa aaatgtgggt agtggggcta aaaaccttgc aagtgctggt aagagtggtt
    26041 taagcaattt aaatgatatt cgtaaaaatg gatttggcaa tcgaaaagat ggtactcctg
    26101 ataacgggga agaacaacca gacgataaag ctacaacagt acctaatagt aaaaagacag
    26161 tttccgaaat gttcgggaaa gcgaagaatg gtgtttctaa agccgcagaa ggtcttggtt
    26221 acgtcaatga acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca
    26281 ccttagaaga aaaattagac ggcacgagag ccaaagaatt ggctgaagct gtgaagaatc
    26341 cgtccgaagc actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag
    26401 atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaggc aaatacaaca agtgaaaaag
    26461 aaaatcaaga aggtgcagcg attccttcag ccaaagatca ccaagatgag ttacattcta
    26521 acgaaaattc gatcggagaa aaagattcag aagaaggaca acatggtcag ttaccaaaga
    26581 ccgatccaga acaatatcgt tataaatcta acattaattc tgaaaacgaa aaacgcattt
    26641 cagatttacc aaaacgaaat caagaagtac aaacggatga ggattccatc gatctgccaa
    26701 atgataatgg catcgaagtt ggaactgaaa aatcaacgaa aattggtttg cctaagacac
    26761 aaaatttacc aatgaaagat agtcgtttga atcaattcaa ggagaatgat caaagctttg
    26821 ttcaccaatc aactgcaggt aatgttcaaa tgaaaccaag tcgtttgcct gaaaatggaa
    26881 atctgacaac tgtttcttac aatttatctg aaagtgggga gacagggaat gcgtccgttc
    26941 aaaatgttgt gacaagagaa gctgcaccac aacctattca aaaagttcac acaggttaca
    27001 agccagcaaa tgtggcattt actgcaccaa caaaaataaa tgtgacacga gaaactaaaa
    27061 acaggaacaa tgaattgaaa gaacaactat tgcgggatat caaacaggat atcaaaccat
    27121 aaaaggccta caagttaggc cttttttcta tgtatgaaaa ggagaaatca tttatggata
    27181 aatatattaa catgaaaaat cctccaagat ttagtgggga atggaagttt ttcgcctttt
    27241 tctttttaga tctattaacg cttggtggtt gctttttttg ggcgaaaata gtcaataatc
    27301 ttgtccactt accactcttt tttgcactct tgaattatgg cttatggatt gcgatagctc
    27361 tagtgattgt ttggcgaccc tataataacc caggtcaacg ccagattttt gcgattatcg
    27421 aatctatttt ttttacggac aagaaccatt accatgccct agatcctaac cgaaattatc
    27481 atggtacgta taaggaggat gaataaatgc gagtcaaagg acaaaactct gttgataaga
    27541 aatccatttt tcagtttgga aaaccagaag aacgatcgat cttggattta tttccttata
    27601 aatcctgtac agaagagggg gtcttagtta ctaaagaaga tcattttcaa cgttattatc
    27661 gtgtagtttc cactgacgtg gagggattaa atgaacaaga gaaactggaa cgaatgaatc
    27721 agttgaccac cgtcatgcgt acgtatgtgc caacaattaa gttagttagt ttgaccacag
    27781 aaacggatct aacggagcaa atcgtacaaa agcggcagtt attagataag aatcgtatgg
    27841 aacaatcctt aggaagaaat ctacagcgat taagaaaata tgaaaagaaa ttagtagagg
    27901 aaatccagga attgaaacga gcagaacaag aacgtcctga cttaagcttc tttttcatca
    27961 ttgaaggaaa gacaatcaag gatttacaaa ccagaaaccg tcagttaatg cgatctagtg
    28021 gcattttagg gttaaaacca ctctacaaaa aagaattgat caagattctt tatcgaatga
    28081 ataatatgaa tgatgagtag gaatggagaa gagagaatga aatatccgtt gatatggtta
    28141 gaaaaagatg ttccgaagga agagaccact agaaaatttc ctcacacttt aagcctggat
    28201 aagttgggaa aagggcaatc ttttcaccaa gtaaagaatc aaacaagaag aggtgactgt
    28261 acatgttaga agcgattaaa acagcttttt caccacaaaa tgatcaaaaa gtacttgatc
    28321 cattaaagca actaggaaat cgggaaatcg aaattttaaa atccaaaggg tatgatttag
    28381 attttttaaa acaagtgatg ccacaaggtg gattaaattt cgatgaagag tatgtgacct
    28441 atggaaatgg ttattcacgg tgtgtgacag tttatcacta cacaaaaaat ccaacacttt
    28501 tttggttggc caatctcatg aacaatcctc atacagtggc cacactcgat gttagcacag
    28561 atgaaaagca aaaagtgatt gatcaaatca atcgtgcctt agatgaatta aatgatcgat
    28621 cagataacga gcgaaaagcg actgaccgaa ataaaagcgc agaagaatac ctggaactag
    28681 cggaatatgc ccgaaaattg acttctaatg gggaaatttc taaacaagtc aaagtgagag
    28741 tacatgttta cggaaacacg attcaagaag tagatgaacg agcgcaggaa ctgattgaaa
    28801 atctcaaagg gatggattat cgtgcggtga cctatctgca taaaaccaaa cgagattggc
    28861 aatcgatgtt taccatgttc gatgaacaag ctgaatggtt cggaaatatc aaagggcaat
    28921 cactaccatc gttaaacatt ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc
    28981 caggaggtac acaatatggg gaaaccatga caggcggccc atttgtctgg aatgcgacgt
    29041 ataaaaatag tgtacggttg tctactaata ccttgcttct aggaatgatg ggagctggta
    29101 aatcgaccgt tcttaaaatg atcgcggaag cccatttagc tgcaggagac tttatttggg
    29161 gctttgaaaa gggaaaagac tttattcctt tcttaaagga atataacggt attatggtgc
    29221 gcttagacgg atctgacggg atgatcaatc ctttagaaat atttgccaca cggacgtatg
    29281 atgaagcatc cagtttatat gacgatggat cagtcaaaga tttgaagatc aatgaagcag
    29341 cgtcctatca aacacactta gacaaagtgg tctatcaggt acagttagtg agtcctcagc
    29401 taaaaggaac gatgaaagca gaatttaaga cctacttaaa tcgtttttat gaagagtatg
    29461 gaacagtacc acgcggcttt acttcctcaa attcgcgttc aaatacggag acgcaagtca
    29521 cagggaaaga tccagaagct tatccgacat tcaaagaatt tttagacttt ttaggacaac
    29581 tggaattacc aggtgcttcc caagagaaaa agaatcgaaa agaagaaatg gaatcaatcg
    29641 tagaatcctt atgtgagacg tatggcatga tttttgatgg tcatagtacg attcgacacc
    29701 ttgaccaaca acagttggtc ttttttgaca ttgactctat tacaggctta gatcgagggg
    29761 tacaacaatg tcaaatgtac gtggcgatga cgttgatttg gaatcaggca ttgattcaag
    29821 gaagaagaca acgacgttta attaaagaaa aacaaatgga agaacaagac ttcaaacgct
    29881 tcttggcgat gattgatgag tgccataatg cgattaatcc agattttatt gaaacggtca
    29941 attatgtcac aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta
    30001 cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga
    30061 agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac
    30121 gtcatataga aggattagtt ggtaaatcaa tgacgaactc agaattagaa tccattcctc
    30181 aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt
    30241 ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa
    30301 tgaaagttta atcaagcagt atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt
    30361 gtttggtact tctgcaggtt tgattaccat tttgatcact gtattcgtga ccctattttt
    30421 aatacttgcg gctggttcga ttgataattc tgattcagat tcaagcagtg gtggcgaaac
    30481 ttttacagga gagtattcgg aaggattgcc catttataaa gaaatcaaag gaagaggccc
    30541 tttttctgat gagattgcgc aatatgccgt aggtgcagca gtgaagtata aattattacc
    30601 aagtgtgatc ctttcgcaat acgggtatga atcagcgttt ggtacgtcgg cgtctgcaag
    30661 aaatgatctc aattattttg ggattacttg gttcgatggc tgtttatttc caaaaggaac
    30721 tgcgcgtgga attggtggca ttgaaggtgg ctggtacatg aagtttccca actcaaaagc
    30781 tgccttctcc tattacggct ttatggtagc cacacaatcc aatttcaatg cttgtgtagg
    30841 aaataaaagt cctggcgcaa gcttattgat cttaggacga gggggttatg ccgctgcagg
    30901 aattacggaa gatagtccgt attatacagg ttgtatgtcc attatcactt ctaataaatt
    30961 aaccgaatat gatgaattcg ccatcaaaca ttggggtgaa ggtggcaata acaacggcac
    31021 gatcactggt gaatggacga atccattccc tggcagcagt ttagataaaa gttccttcag
    31081 tggtggtcaa ttatttggaa caaatccagg cggtgaattc cgaccaaatg gtttccatga
    31141 tggattagac tttggttcag ttgatcatca aggaagtgag attcatgcgg ttcatggagg
    31201 aaaagtcgtg tacgtcggaa atcctggtat ttcaggtttg ggcgcgtgtg tgatcgtgat
    31261 taattacgac ggcttgaata tggtatatca agaatttgca aatagtacag gaaattcccg
    31321 tgtgaaagtg ggagatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca
    31381 cttgcattta ggtttcactc gaatggattg gcgtcaagca caaggacacg cattcacagg
    31441 tgatggtaca tggatcgatc cattaccatt tttgaatagc agcaaaaaat agatcaggag
    31501 aaaaaatatg gaaaataaaa ctttaaaaat cgtcttaaca gctgcactca gcattagctt
    31561 aattggcaat atcgcgctct tttggcagct gaaagaggaa caagcgacaa atacctataa
    31621 aattgaagaa gtggctaaaa acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca
    31681 ggcgaagatc gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tccatcagct
    31741 gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc
    31801 agaagagttc ggtcaattgg ccattgataa atcattaagc agaaatgaat taacggaaaa
    31861 actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag acgataatca
    31921 ccaacatgat gattacggaa gttactcttt tacattaggt gacgtgcaaa cttacgctca
    31981 aacaagcagt gtaaaagagg aacaaaattt tgttgtattt gttgattata caatcggcaa
    32041 tcctcaattc aaagatgtca aaccgcaaaa aataaaaggt ggattaactg tcactgaaaa
    32101 acaagtagat ggtcaatgga aagtcacaga ctttagttat tttactcgtt aggaggaaga
    32161 aaatgagtaa gaaaaaagga gcaagagaac gtttcaaaaa acgaaaaagt tatcttagat
    32221 ggctgggaat ctttatgatt ctcggctttt taattcttgc tagtccgatt tatacgcata
    32281 agaaggtaga cataccagat gaggtaagca cgaacgcatt tttaaacacg aaggaactga
    32341 cgatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcgatcgacg
    32401 atgaagatca aagcttatta aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga
    32461 cagctaaagg ggattatcaa gcaataaaag ccaaagtgat caaggtgtct gatgattatt
    32521 ttgtagtgaa gttagcacat gtaccagaag aatatatcgc catgcgttta accattattc
    32581 ctgaaaaaat tgatcctaaa gtggatatgc aagaaccaca agatctgatt tattacgttc
    32641 acgaagataa ggttaaagat caagtgaaag atgaagatta tgaacaacat gcgatcaatt
    32701 acaaagtgaa aggatacaag aaagaacaaa aagcagcaca aaaacaaatt gaatccttcc
    32761 aagcaaccat tgatttgaat gaagaattag tgaagaaact aaaagatcaa ttgcattatc
    32821 aagttgcaga cgatcaggaa aatactgaaa ataagattaa tagttaccag caagaaattg
    32881 aaacatcgaa aacgcaaatg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa
    32941 ttgatcgtat gacaaaagaa aatatttaga agaataggtg gagtaaaaga aatgaagcga
    33001 gcgaacttgg tcggcaaaga tactttcgct gagcagaaag tattgattaa agcctatctc
    33061 aaagttgtaa aagctaaaga aatatgtcaa gaagtgcatt tatctaacac aacaatatct
    33121 gagttgaaga atggtagaaa gagtttagat accttaaaat tcagatcatt tgaagctttg
    33181 tatttagctg ctaaaaaata ttttacagct gataataaat aaaaaattaa ggtgggttct
    33241 atgtgaaaaa aacgtatcaa aagtttttat ggattagcta tatcgtccta tttatcgttg
    33301 ggtttctcgt cagtgtgtcc gtgatcgtcg gaacgctttt tcttttgttc tctgacgaag
    33361 atctggtact taaggggtta atgagtacgt tatcgtgtgt catgttactt tatttatgta
    33421 tacagatcaa cgaaattatg ccccctcatt ggcaagaaaa gaagaaagaa tttaaggaag
    33481 aaatcgaaat aaaatccaat gactggattg aatacacatg gcttcctctg aaaagaaata
    33541 gcaaacgaaa ggaattttgg gcattgcaat tatggccaat aggaaaactt tttatttata
    33601 tggcttcact tatggttact ttaatcggtt atttggtggt ggtctatggg gtggcctatt
    33661 acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca gtaatgacat
    33721 taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt atcaggcaaa
    33781 atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta ggcagtatgt
    33841 taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg ataaagaact
    33901 tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa gtaggcttta
    33961 ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc gaaaaagaaa
    34021 tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat tttggaaagt
    34081 atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta accgttaagg
    34141 gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccga agaaaagagg
    34201 tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa ttgaggttag
    34261 atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg atttagaact
    34321 tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct ttacagaatt
    34381 gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt caaagtgaaa
    34441 gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca ataccgaaca
    34501 gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca acataagcaa
    34561 gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt caaggaatct
    34621 aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa acgtgacccc
    34681 acttttaaaa aaagtggttg gataggctgt gcctatcacg tttctgaaaa aaataaatga
    34741 ccccaaaata ccaatatata ggatagtaca aggatagcaa aggatagtaa aaggatagag
    34801 gtaggatagc gaaaaggata ggatcctttt cactatccta tattatattg gaggatattt
    34861 atgaatgtta aaattagaga tttagatcca aaattcattt ctgaaattga tcgaagatgc
    34921 gttgaactaa gtaatcgtac aggaaataaa tggagtcgta atgattattt aaagctactc
    34981 gttgaaaatg attttgatcg tcctttgatg gaatacaaac aagaaaagtt tgaccagtta
    35041 ttagaaaagt tttcagaaat tcaaagttat aacacaaaga tattagaaga atatgttagc
    35101 caaaataatc gtattataga aatcttgatt gagcaaaata gaggaagtga actttgatat
    35161 ggcaaaggtt atagacaaac acatttactt tcaccaaaca catatcgatg tattggatga
    35221 aattatttct actcatgcag aggttaaaaa ctatagtgaa gctgttcgct ttttatgtat
    35281 gaactatgat ccagaagtgg agaaaaaagt aaattccaat caagcaaagt tgaatggaat
    35341 gagtaaagaa atttctattt taaccgaaat ggtttcagaa tttgcggatc ttcatttgaa
    35401 agatacagga atattaattg gtaaaaattc aactgtttat caagaagcaa aagaacgagt
    35461 ggaaggaaaa attaaatcca atcaaacaaa aaaatattcc cctcagttga agaaacctgt
    35521 gaatgaatct gttcatgcca gagtagatcc atttgatcca acgaagattc taaaaagata
    35581 aaaatgaagg aggataataa tgactcaatc gccttcagtt attctcactt gtcaatttac
    35641 tttgccgaat aaaaagtcat tttctactta tattgactat attacaaggg aacaggctct
    35701 tgaagaaaag gaacgacgta cgcccgaaga agaacaggaa cttcaacgaa tcaagaatgc
    35761 tttagaggag ttttatattc ctgaaggaga aactttttcg gagagtaaga cgggtaaaga
    35821 attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca attttgacaa
    35881 gttagaggaa caagatttta ccaattatct ttcttatatg acacgtcact atgcgttaga
    35941 acaaaagaaa gaactgacgc aaagtgaaca aaaagaatac caacgcttga accaagcaat
    36001 gaaaaaatat caaacagaac cccataaaaa agcttcagga aatcttcctg gagttttttc
    36061 tatagcttca gacgaagtga aaggaagtga tctgaaggag attaagcaaa tcttccaaag
    36121 aggacaacaa aaaggctcaa tcatttatca agatgttgtt tctcatgata attcgtatct
    36181 tgaaaaaata ggattgtacg atcccaaaac agatacttta gatgaagaag ggttaaaagc
    36241 agctggaaga aaaatgatgg agacattatt cgagaaagag caactgaacg aaacaggcta
    36301 ctggatggca acgattcacc gtaacactaa acatatccat attcattttg cagttgttga
    36361 aaaagaaaat acgcgtaaac cttacacgga ggttgaaaat ggtgtgagtt atatcgtacc
    36421 gaaaggtaag agaagacaag caacattaga cgcaatgaag accaattatg tacatgagtt
    36481 agaacgtttt tcttatgaaa aggatcgaat gattggtatg gagaaacgaa atttattgac
    36541 gcggaaaagt gatttgcgta acaccttaac aaaagtggta aaagagccag cgcgttacga
    36601 tcgacacgcg atgaacttat taaatgaagt atataaaacc ctacctgaca aacgttcgga
    36661 atggaattac ggggatgaaa aacgaaccaa actttcacca tacacaagag agaaactcga
    36721 tgagttaacc aaatatgttt taacaaaaga acctgaatat caagaatata tacagatttc
    36781 aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga
    36841 tgcagagaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca tcctaacaga
    36901 gctgaagaga caggatagtc aaataaaacc attaatagaa agtgttgatc agatgcaatc
    36961 tatttacaat gaacgatcct ttcgaacgct taaacaattg ccaacgaatg aatacaaata
    37021 tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcagcg
    37081 gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa
    37141 agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt aaagacagta
    37201 ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg atcattaaga
    37261 gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt ccagataaga
    37321 gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagtcgttga gtacccagct
    37381 actggtgtgt attgttgtaa tcatgtgaat ggtgaaaaat attttatcat tcaagaatat
    37441 gagggcaagt tagcacctta ttatactact tgggaaatga atgaagaggg aatcaataac
    37501 tttccatgta aaagcattga agagtccatt tcactcacag agtgttagtt gttaagtgaa
    37561 taaaagaagt aaaagagtcc tgaacgtgat tgtccaggac tctttttttc aatgaattgt
    37621 tttccgccaa ctcattgagt ggtgcgattt gcgactacat tatatcaaaa gaaagagaag
    37681 ggaggaagca gaaatggttc attcaacgat tggagttgtt gttgaaaaaa gcagagacaa
    37741 tctcgttttt gtgacagaaa ttcaaacagg cagatctttt gtcgtcacag acaaagcggc
    37801 aaaagcttat caaagtagcg atattttaac gttaaatctg actacgaaga cgttcgtcga
    37861 tgcgacagaa gattatccat ttgtttagtc taatggtgat aagtcaagat gaaatttaag
    37921 attttcttta ttgagtaata tgttttgaag cgcacgaaac tagacccgat caaaatcaaa
    37981 tttttttcgg gttgattcaa ggatttgcgt caataacgga ttgttattt
//
LOCUS       DACCQP010000021        36000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCQP010000021 DACCQP010000000
VERSION     DACCQP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.021"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            391..840
                     /locus_tag="IVO87_001189"
     CDS             391..840
                     /locus_tag="IVO87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7449395.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            897..2315
                     /locus_tag="IVO87_001190"
     CDS             897..2315
                     /locus_tag="IVO87_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449396.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            2315..3175
                     /locus_tag="IVO87_001191"
     CDS             2315..3175
                     /locus_tag="IVO87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7449397.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            3349..4122
                     /locus_tag="IVO87_001192"
     CDS             3349..4122
                     /locus_tag="IVO87_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7449398.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            4269..4634
                     /locus_tag="IVO87_001193"
     CDS             4269..4634
                     /locus_tag="IVO87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7449399.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(4782..5990)
                     /locus_tag="IVO87_001194"
     CDS             complement(4782..5990)
                     /locus_tag="IVO87_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7449400.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(6009..6287)
                     /locus_tag="IVO87_001195"
     CDS             complement(6009..6287)
                     /locus_tag="IVO87_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449401.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            6480..7136
                     /locus_tag="IVO87_001196"
     CDS             6480..7136
                     /locus_tag="IVO87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7449402.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(7578..8375)
                     /locus_tag="IVO87_001197"
     CDS             complement(7578..8375)
                     /locus_tag="IVO87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7449403.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(8372..9556)
                     /locus_tag="IVO87_001198"
     CDS             complement(8372..9556)
                     /locus_tag="IVO87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7449404.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(9581..10828)
                     /locus_tag="IVO87_001199"
     CDS             complement(9581..10828)
                     /locus_tag="IVO87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7449405.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            11043..12788
                     /gene="dnaX"
                     /locus_tag="IVO87_001200"
     CDS             11043..12788
                     /gene="dnaX"
                     /locus_tag="IVO87_001200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7449406.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            12808..13122
                     /locus_tag="IVO87_001201"
     CDS             12808..13122
                     /locus_tag="IVO87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7449407.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            13147..13743
                     /gene="recR"
                     /locus_tag="IVO87_001202"
     CDS             13147..13743
                     /gene="recR"
                     /locus_tag="IVO87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7449408.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            13807..14451
                     /locus_tag="IVO87_001203"
     CDS             13807..14451
                     /locus_tag="IVO87_001203"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7449409.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            14466..14795
                     /locus_tag="IVO87_001204"
     CDS             14466..14795
                     /locus_tag="IVO87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449410.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            14809..15747
                     /gene="holB"
                     /locus_tag="IVO87_001205"
     CDS             14809..15747
                     /gene="holB"
                     /locus_tag="IVO87_001205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7449411.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            15783..16607
                     /locus_tag="IVO87_001206"
     CDS             15783..16607
                     /locus_tag="IVO87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7449412.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            16600..16947
                     /locus_tag="IVO87_001207"
     CDS             16600..16947
                     /locus_tag="IVO87_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7449413.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            17016..17888
                     /gene="rsmI"
                     /locus_tag="IVO87_001208"
     CDS             17016..17888
                     /gene="rsmI"
                     /locus_tag="IVO87_001208"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7449414.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            17997..19118
                     /gene="dinB"
                     /locus_tag="IVO87_001209"
     CDS             17997..19118
                     /gene="dinB"
                     /locus_tag="IVO87_001209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7449415.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(19172..19774)
                     /gene="nrdG"
                     /locus_tag="IVO87_001210"
     CDS             complement(19172..19774)
                     /gene="nrdG"
                     /locus_tag="IVO87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7449416.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(20089..22278)
                     /gene="nrdD"
                     /locus_tag="IVO87_001211"
     CDS             complement(20089..22278)
                     /gene="nrdD"
                     /locus_tag="IVO87_001211"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7449417.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            22604..23371
                     /locus_tag="IVO87_001212"
     CDS             22604..23371
                     /locus_tag="IVO87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449418.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            23361..25403
                     /locus_tag="IVO87_001213"
     CDS             23361..25403
                     /locus_tag="IVO87_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449419.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            25684..25833
                     /locus_tag="IVO87_001214"
     CDS             25684..25833
                     /locus_tag="IVO87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7449420.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            25844..27358
                     /gene="dltA"
                     /locus_tag="IVO87_001215"
     CDS             25844..27358
                     /gene="dltA"
                     /locus_tag="IVO87_001215"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7449421.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            27355..28560
                     /gene="dltB"
                     /locus_tag="IVO87_001216"
     CDS             27355..28560
                     /gene="dltB"
                     /locus_tag="IVO87_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7449422.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            28605..28838
                     /gene="dltC"
                     /locus_tag="IVO87_001217"
     CDS             28605..28838
                     /gene="dltC"
                     /locus_tag="IVO87_001217"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7449423.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            28841..30109
                     /gene="dltD"
                     /locus_tag="IVO87_001218"
     CDS             28841..30109
                     /gene="dltD"
                     /locus_tag="IVO87_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7449424.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            30250..31332
                     /locus_tag="IVO87_001219"
     CDS             30250..31332
                     /locus_tag="IVO87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7449425.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            31332..32135
                     /locus_tag="IVO87_001220"
     CDS             31332..32135
                     /locus_tag="IVO87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7449426.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            32205..32945
                     /locus_tag="IVO87_001221"
     CDS             32205..32945
                     /locus_tag="IVO87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7449427.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            32956..33960
                     /locus_tag="IVO87_001222"
     CDS             32956..33960
                     /locus_tag="IVO87_001222"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7449428.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(34005..35849)
                     /locus_tag="IVO87_001223"
     CDS             complement(34005..35849)
                     /locus_tag="IVO87_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449429.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        11501 a         6285 c         7407 g        10807 t
ORIGIN      
        1 tttttatgtt ttttgccaat ttaaaacttc gtttgatgct gaaaaagtcg taggatcata
       61 atgcacaaaa caatgaaaaa ctagtatata gttttaatta tccacttgat aaaaacacct
      121 tatattaacg aaatgaagag gggttatttc gaagtaaaaa accatttacc taaaattaga
      181 agctaagctt tcctttcaag gaaggctttt tgttttgtta tagacttgcg attgtttgtg
      241 agctagatcg ttcattttaa tgacttatac acgcggatga tgggtctttt tcctctgttc
      301 attcataata ttgaacggta cagctagcga tgctatactg acctcagttc caagaaaacg
      361 ttaatatttt ttgtggggga gaggtttttt atgaggtttg atcaattgat cgcagaagaa
      421 ctgattgttt tcgatccatc gatagattca aaaggatcat tatttgagcg aataggggat
      481 gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag tcgttaaggc actattcaaa
      541 cgagaggagg agatctccac aggaatagaa gatggatttg ggattcccca tgcaaaaagc
      601 aaatatgtga caaaaccagc actgtgtttt tttcatacag ccaaattaac ggactatagc
      661 gggatagatg aaatgccaat tgaatatatt tttgccatcg tagttcctga aaaatcagca
      721 gatatgcatc tagagattct cagcacactc tccagacgat tgatgaatgc agattttaga
      781 aagggaataa aagaagccac tacgccagca gatatcatca gacagattag tcaagaatga
      841 aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa aaggaggaaa ataatcatga
      901 aaatcgttgg tgtaactgct tgcccgactg gaattgctca tacttatatg tcggcagaaa
      961 aattagaagt aacagcaaga aatctaaacc atcaagcaaa attcgaaaca caaggtgtaa
     1021 aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc agatgcaatc attcttgcag
     1081 tagataaaga aatcgaattg gaccgatttg ccgggaaaaa agtaaagcgt gtctctacaa
     1141 gccgagcaat caaagaaccg caagtagtaa tagacgaagc attgagagat attggcgtat
     1201 tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa aaagccgaca atctataatc
     1261 atttcatgaa tggtgtgaat tacatgcttc catttgtgat cgcaggcgga atcatcatcg
     1321 cactgagttt tgcctttggg attacagcgg ctgatcccaa ctcagcagac tataatgtgt
     1381 tagcagctgc attttcaagg atcggaggag atacagcgtt tgccatgatg gttcctgcgt
     1441 taggcgcggg gattgctaca tcgattgctg gaaaagcagg ttttgcccca ggaattgttg
     1501 ccggattact ggcctctaca ggtggttcag gttttcttgg cggaatgatt ggtggcgtgc
     1561 tagcagggta tatctgtgat tttctggcaa ataaaattag cgtaaaaaaa gaattttctg
     1621 ctatttatca gctgattgtc gtcccctttt tatcgatctt aattattggg cttttgatgg
     1681 tatttgtgat tgaacaaccg atcacatggg tgttggatgc tttgacaaat tggctgaata
     1741 gcttgggaaa tacatccggg ctattgttcg gattacttat cgggatcatg atggcagctg
     1801 atatgggtgg gccgatcaat aaatcgactt cgactttctc gatcggtttg atgtcagctg
     1861 gagtaaatgc accgattgct gcttgtatgg ctgcaggaat gacacctccc ctaggtattg
     1921 ctttagccac tttattattc aaacataaat ttaccaaaga agaacggact tcaggtcaat
     1981 catgctggat tttaggcgct tcgtatatta cagaaggtgc tataccgttt gcggtatctg
     2041 acccgatccg cgtgatacct agtttgatga caggttcagc agtcgctgca gggatttcca
     2101 tggcagtagg agttacttca atggcacctc atggagggat ttgggtgatg ctaattccaa
     2161 atgttatgaa caatttacct atgtatgtct tggcaattgc atcaggtaca gtcactacag
     2221 cctttatgct tggactattg aaaaaaacag tgaaagagaa aaaacaagac aagtggacga
     2281 aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta tatacaatga aagagttatt
     2341 gaaagtggct aaagagaatc aatttgcagt accagccttc aatatttgca gctatgatat
     2401 gatgaaagca atcatcgaag aagcagagag attagaagca ccagtaatct tagaaattca
     2461 tcctgatgaa atcgcctatt taagtgatga gtttgttgcg gcagtaagag cctatgctta
     2521 tacaagtaaa ctgccgatcg ttatccactt ggatcatggc ggtacgatag aagatgtctt
     2581 acgggcgatt cgaaacggct attcttccgt gatgatcgat gcgtctttga aatcattcga
     2641 cgacaatgta cagattacga aaaaagtggt tgcgttagct catgcggtaa atgtctcagt
     2701 ggaggctgag cttggaacga ttgggaataa tggttctgct gaaggaggtg ctgaaacaat
     2761 catttataca gatcctgatc aagcgaagat ttttgtgaaa gaaacaggaa tcgatacact
     2821 tgctgtagca attggaacag cgcatggctt gtatccgaaa aacagaactc caaaattaaa
     2881 tatcgactta ctgaaagaat tgaaccaaca aatcgatctt ccatttgttt tacatggcgg
     2941 ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa tacggagttg ccaaagtgaa
     3001 tttgagctca gatcttaaaa gcgtcttttt caatgctttg agagaaatat tgagtgaaaa
     3061 tccatcgatg tacgagccga atgcaattta tccagcagct aataaaaagg tacaagaagt
     3121 ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag gctagtttat actaaaaata
     3181 aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat accctcttaa ccacacacga
     3241 tgttcagaag tagttttttc gaaaaatttc ttcttatttc tcgaagccaa tcacttctgt
     3301 cacaacctct tttttcctgt tactatataa agtaaatggg gtgattttat gttgaatgaa
     3361 gtggagaata gacagcagga gattgtgaat tatctaaaaa acaaccaatt tgcaagagtc
     3421 accgatttga tcgagttagt aaattatagc gaagccacga ttaaacgtga ccttgttttt
     3481 ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg ccatgcttat tgacaatcga
     3541 aaaattgacg tgccttacct gatgaagatc accaaattag aagaagaaac agaaaaacgt
     3601 aagattgcag atacagcttc ttctttgatt cgagatgata tggtcttatt cttggattcc
     3661 agtacaacct ctttacactt agtcagaaac ctagcaagat ttgatggttt gcagatcatt
     3721 acgaatggca tcgtcacagc tgctatgttg agtgaatata caacggcaaa agtatctgta
     3781 ttgggagggg caattgttcc gaaacgagca acaatcaacg gttcaaaagc ctataacgat
     3841 gcattgtcat attatgcgga tttagcgata atgagttgtc gcgggctaga tttcagccaa
     3901 ggagtaacag aaactcatga aggagaggca atggtcaaaa aagcttttag aaaacaatca
     3961 gaccggcttg ccgttttagt tacacagcaa aaaattgcac ataaattcat gcatcaagcc
     4021 attgcttttc atgaaatcga ttatctggtg acaagtaaag aactgacaaa agaacagtat
     4081 gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat aaattattac gtgaatgttc
     4141 taagtggctt cctcattttt agataggacc ttatctttgc tcgcttctct cagccgatgg
     4201 tataataatg aaaaggttat cttgttagat aatcttactt ttttatccag gagaaaggag
     4261 tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc aatcatgatc tgggcgattc
     4321 tgtgggtcgt gctgacaggt ttatacggtg ttagctttat ggattcaacg attgttgggg
     4381 taattattgt agtcatgatc tatgtgctag gggatctgtt gatcttacgt aaagtaggga
     4441 acatcgcagc gacgatcgca gatgctggtt cagctgcagt tatcttgtgg ctctatctat
     4501 attttatgaa tgatacagtc gatatttgga tgaaagtatt gattcctgct ctattgatcg
     4561 gtgtggcaga atggttcttc cacaagtggt tgctaagtca aggcatcgta ccagatgaac
     4621 ggaaaatgga ataaatcaac agttaaacta ttcttatcga taaatgaaag ctttaaaaat
     4681 aaatagatgt ttaattaaga ataatcataa actattttta ctcaaaaaac cgctcagatt
     4741 attttcagat ctgaacggtt ttttttatct attcatcaag atcaaatttc atttttcaat
     4801 gattcaatca attcgccctc tgcttcatga ctatgttttc ggccattaac aagatagagt
     4861 gctgtacata tcaatggaat gactccggca atcacataca atcctctaaa tgaagtaaga
     4921 gattttagca tccccatcaa ataaggacca acgcctaagc ctaggtctaa accaataaag
     4981 taggtagata aagcaatact gattcgatgt tcgcggacga tttttaaaca aacagcctgt
     5041 ccatttgaca taaacgtacc ataacctagg ccgactaatg ctccagagat aagcaatgtc
     5101 acactattcg tcgttacact cagtaaaaat aatcctgctg ttaagaataa atagctagga
     5161 tacatcacgt attgttctcc tttgacatcg aaaatccttc cagtcaatgg acgtgtaaat
     5221 gtgatgacca aagcatatac gacgaagaaa aatgacccag cagcaactaa gtggattact
     5281 tttgtataag aagataaaaa tgataaaaca cttgaataag aaagacccat caaaaaggca
     5341 attcctgtaa tgaaaagtgc tttcttttca ataaaactat tgatattcca tgagcgaaga
     5401 gatatttttt gttcttcaga taatggaata ttttttacag gaaagacaag gcaggcaatc
     5461 gtaacaaaga agaccagtac aattgaaaac cagataatga aacgaaaatc agtcatattc
     5521 agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta aactagtact taatccataa
     5581 taattgatgc cttccccttt tttagatgca gggatatatg cagtaacgat agcatttgta
     5641 gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc gaactaaata caatgccccg
     5701 atagttggcg tataaagata tgctaatgtt gaaaggagat agaagatcgc gccgcctctt
     5761 aaggtaaact tccgaccaaa tagttctaac tgtttcccca tgaataagcg tgccagcaat
     5821 gttccgatga tatagatccc agaagcaaaa cctgcttggc tcaagctggc gtgtaaggtg
     5881 tcttgcgcaa tgactgccat gatgaccatc aacagataat aaatcaggta gacaataaaa
     5941 ttgatcaatg tgattccaat aaaatattta ttaaataatt tcttttccat gtatgttata
     6001 cattcccctt aagctgaata tgcttttaat ttttgtattt gaacttctac atcgtgataa
     6061 ttggatggat agcgatcaac aagttggcga ataaataaat ctcttgtaga cataaacaga
     6121 tttaaaaatt cttcgcattc gtttgttgga ttctcatcca ctgctttgat caattcgatc
     6181 gagttttgat acgcatcatt taaaaagact aaagaagctt tcatatcaaa tcttgtttga
     6241 tatgctgttt ctaatcgatt aatgattgtg ttcatccagt aattcatgtt ttttccctct
     6301 attcttaaaa aattttactt ctgcagtata acgaataaaa aaaaggaaaa aaatcgcata
     6361 agtgatggaa aaaggtactt aacaaatgtt aatatcatta gtaaaatacc tttggacttt
     6421 aaaaagtttt aaagtactgt atgatctttt tagatgaaag tttggatggg ggagcatcat
     6481 tggatagtag agtcttaaag gaatatttgg ataaaaatga ttttccgatt atacgcaaag
     6541 gatatcataa atatttgaca ttcgaaggga tggaagattc ccatacgtat atcttgaaag
     6601 agggaacggt aaaagcaagt gttatttcaa atgatgggag agaatttaac cttcgctata
     6661 tcaaagatct tgaaatcgtc tctcttttaa aagacgaata ttcacaattc atcgattcgc
     6721 cgtataatat tcgagttgaa tcagaatatg cagagtttta tcgaatcaat cgtgtgcgct
     6781 tttggcaaaa tatcaaccaa gacccaaagc tacaacacta tgtaaaagag tattaccgct
     6841 atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct attaaacgga aagctgggag
     6901 ctgtttgtac gcaactttac gaattatatg atttattcgg tgtgcggaca gaagaaggga
     6961 tgctgatcga tttttcgatc acgaatgaag aaatcgccaa attttgtggc atctcaactg
     7021 ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga tggtattatc accgttaaag
     7081 accatcatat cgttattttg aatatggact tgttagaaga caatattatc ttttgatgaa
     7141 caaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac ggaaaaatag
     7201 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
     7261 accaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca
     7321 gcttttcatg tttgatgaat attgtgactt gtgaccgagg agttggtagg agaacaccgt
     7381 ttaccaagag gttgtgaaaa agctgttctg catcaagtaa taagaagagc caagtaaaaa
     7441 cagtctctta tgtttttaac ttggctggac ttatcaccgt agatgtaagc ttttgaacgc
     7501 cgtttatttg caacaaaagg ggctgtgaca cgatttttgt cacagccctt tttttgcgaa
     7561 cattcattta gcaattttta gaaacttctg ccgccaccgc caaaagtccc gccaccgcta
     7621 gaatgggtag tacttccgcc accgccgctt ccaggaggtg ggcttttggg gattcttctt
     7681 gtcgtgacaa aagaatttgt cagacgatcg gttttttcag ttaatttgat actggatttt
     7741 tctcggaatg gatatttgta gatgcctgat ttcaattgat aacgcgaaac agtcacaaaa
     7801 taaaagacaa gactaaggat aagagccaaa acaacagcaa tgatgatttc tactgtcgtc
     7861 aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta cacgataatg tcctcctgga
     7921 actccgtctt ccacatatgt tgctgattta gacaggtaag cttttgcagc attgtaatag
     7981 gcagcatttg tcatctgatc ataaacatca tctaagatcg aatcgattct gctatcagtc
     8041 aaataatcga tcatattacc agaagtcgaa atgtagattt cacgctgtcc catatctaat
     8101 agcaatactg agccattttg atcatttccg actgcgtctc ttaagtaatt atcagcaaag
     8161 tctcttggtt cttctgaatt agatgtagtc gttacgatca agactcgtcc tttgattttt
     8221 tcattcagct tttctgcctg ttgttccaaa gattggattt gatctgttgt gaataaccca
     8281 gcctcatcat ttaccgtagc tgtatctgca tgagtagcca ccggccagcc aacacttact
     8341 agcaaaagaa agaataaaaa aaattttttc atcatatcaa gtaacctccg atcagaaaga
     8401 ggatcgctag cccagcgaaa atcccaagag cggtcagtcc caattttttc tggctgatcg
     8461 gcaggacacc gctgaccttt cctgtttgtc cattcattgc gtagtagtag acttttttac
     8521 ttgagtcatt gcttcgataa gtaacaagcc ataccggcag taatacgtaa ttatttactt
     8581 cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt tgtatatcca tttgcggtat
     8641 cttttaacag cttctctgaa tagtctttta attcagattg aatctgtgat ttgattgcct
     8701 catattcgat atctcttttt tctgcttgga acccggctaa atactggctt ttaaaatcaa
     8761 ttgctttatc taacgggaaa ggttgcacgc cttcgaccat cttttgttgc gtattttttg
     8821 ataaggcatt ttttactagt tctttaaaag aaagcttgcc tgctcttgaa acagcaaatt
     8881 gcttcgtttc tgtgtattcg atgtcaccta tccgccatat gcggataact gtaccattcg
     8941 cctgcatggc accatctact tcagcatctg tagcccaata agggaaatac acacctgtca
     9001 acttatcgat ttgatcctta ttgaaaaaag ctttgggaac aaaccatttt ttcttggtcc
     9061 aagcgagaaa tctgttgatt gcttcttctt tctcgacagc aaaaggaagt actttatttg
     9121 gcaagaattc tccgcttaaa cgtcccgata gtacgacggg attatggcag tagtaacaat
     9181 aagtcgcagc agttgtcgca tcagtcacga tctctgcacc acagttggga caaagaaaaa
     9241 gatctatcgc tgcggtatta tctgcagatt cgtccgctgt atctgaaaaa ccgcctgcct
     9301 cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag ctgttcttgg gctgtaaaag
     9361 taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc gccagccata cgcgcttctt
     9421 tttgcttttg ctcatattgg ctgacttctt cttctgtata gatattcagg cagtattcgc
     9481 aatgaaattt ttggtctttg ggatcaaaag tcaacggacc gccgcaatta ggacatttat
     9541 gtgtaagaac atccatcagc tgcacacctt tctaaataaa ttaattaacg gggattcctt
     9601 taaacggttt accacagttt ggacagaatt tagggatgcc gttggaaaga tccacgatct
     9661 cattgcattc actgcatcgc atttgtagct taggctgaac gttttctaca ggtttggcag
     9721 ctccgcagtt ggaacagaat tttccggaat tttctgtccc gcaaactgga catgtccaag
     9781 tatccgcatt tccttgtgca gtttgttgat tttgtttttc tgcttgttgt ttgatttgtt
     9841 cttggttatt ttgagctgct tgagaaagat aagaaccatt tgcattcatt cccataccta
     9901 cacccataaa gccggtcatt gctcctgctt cattttttcc ggctgcttcc ataccacgtg
     9961 caatggaacc ttgcacgtat ccttcacgta cgctaggatc acctagcatc gccccttcgt
    10021 tacgcatatt gatcagtttc acagaatcat cggtgtaaga aatgctggct acagccacag
    10081 aaacaatctc catcccacgt aattcacgcc aagaatcatc caatgcagta tccatgtatt
    10141 tgcttaattc caaactctta gatggtacat aagaaatccg ttgaccatct gcagacatct
    10201 gattgattgc tgattgtagc gctgttagga attcagctaa atactgctca ttgatatcat
    10261 tgatttccac ttgtgttttg tttttaggga ttgcgtttgt ataaaataaa atcgggtcaa
    10321 cgacatgaat agaataagtg ccatgtgccc gtaagaataa ttctgcgtta tagaaattgt
    10381 caaagtagtt caacggcgcg cttgtaccga atttgattcc ctttatttct tggagattga
    10441 tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa tttgaatcgg tcaaaagttt
    10501 cagcaattgc ttctttcagt gttccattga acatggaagg agctgcgtca tttttaatgg
    10561 tgtaataacc ttcctcagcg gtataatcga tgatttttcc accatcgacc aaaagcatca
    10621 tcatgtttgg atagacatgg acgactgttc cgtctgttaa aacatcttct gttcctttac
    10681 gattggagcc acgtttgtca tctttgcgga ctttgacacc ttttgtcatg accgttgtat
    10741 cactcatatt gtctggttcg atgacttcta gccattgatc tgccagaccg ccgccaacca
    10801 tacttgttgc tgctttgata agacccataa aaaattcctc ctctgattca gctagtgcca
    10861 aacaattatt gtttatttat tataccatag gaagatgtga aacttctcgg actagagacg
    10921 gaattgaaat ggaaaattgc gtattgttta aatacctgaa aaagccgaga aggatagtag
    10981 tttttttacc taatatcacg tataataaga gatagtgaat tttttaaaaa agggggatcc
    11041 ttatggcata tcaagcactt tatcgagtct ggcgttcgca gcgtttcgaa gacatcgttg
    11101 gacaaaaagc tgtgacacag acactaaaaa atgctattgt ttcccacaaa acctcacatg
    11161 cctacttgtt tacaggaccc agaggaaccg gaaagacgag tgcagcaaag attttcgcaa
    11221 aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg caacgaatgc gaaatgtgtc
    11281 gatcaattac tgctggtacg caagaagatg tgatcgaaat cgatgctgcc agcaataacg
    11341 gagtcgaaga aatccgattt atccgtgatc gggcaaatta tgcgcctaca aaggcagcgt
    11401 acaaagtgta cattattgat gaggtacata tgctttcaac aggagctttc aatgccttgt
    11461 tgaaaacatt ggaagagcca aaagaaagtg tcatattcat cttagcaacg accgaacctc
    11521 acaaaatacc agctacgatc atttcacgga ctcagcgatt tgattttaaa cggatcaata
    11581 cggcagatat tgtcgaacat cttgaattta tcttgaaaga atcgactatc agctatgaac
    11641 cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg gatgcgggat gcattgagta
    11701 ttcttgatca ggcaatctcg tttagtgaag gaacaattac tttagatgat gccatgcaag
    11761 tgacaggtag cttaactgac gaaatgatgg acagttactt gagtgcttgc gtgaaaaaag
    11821 aagtaccaga agcattggaa acattgaacc aaattctcgc agccggcaaa gaatcacgtc
    11881 gctttctaga ggatatgtta caatactgcc gtgacttgct gatgtatcag caagcaccta
    11941 acctagttga ggaacaatcg agtacgatca gaagtacatt tacagaattg gcacaaactg
    12001 tttcagcgga tcagctgttt tcaatgataa agattttgag cgatactcaa aatgaaattc
    12061 gttttaccaa tagtgccgct atttatttgg aagtcgctac agtcaaactt gcacagctac
    12121 cgtctgcttc tgttccttca gcagatgtag catttgcaga tgatcagata gtaaaacagc
    12181 tacaacaaga gattaaggaa ctaagaaacg aaatcaagga actacgcagt aatacaggaa
    12241 ttgagaatac aaaagtacag ccggtacagc aaacaaatca taaaaaacag acaagtacct
    12301 atcgtgtacc gacggaacga gtatataaag ttctgcggga agcgacaaga cagcatctga
    12361 accaagtaaa agaagtatgg gaagacttgc tgatgagtct atctgtcact cagcgagcaa
    12421 tgcttaaagc aagcgaacca gttgcagctg gacctacagg actagtgatt gcgtttgatt
    12481 atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt gcagctttcg gtacacaaca
    12541 acttaagccg aatgatcaaa gattatgcgc ctgatcctgt ttatattaca agagaaagct
    12601 ggccagaact tcgaagaaat tatttgacgc aggcacaaga agagggaaca gaaaactctt
    12661 ttgcttctga agactcagaa ggagaaatcg agttgcttcc tcaagaacaa gaagtaccag
    12721 aagaaacact tgtagtaaaa gcagaggaac tgtttggctc cgaagctgtt acagtggtag
    12781 atgattaata aaaaggagta gataattatg atgcgtggaa tgggcaatat gcaaggcatg
    12841 atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag cacaagagct gctaaataaa
    12901 acagagttta caggtaaagc caccaatgaa ttggtcaaag cggtttttac aggcgatcgt
    12961 cgcatgaaag atatccaaat caatgaagaa gtcgttgatc ctgatgatat cgagatgctt
    13021 caggatcttg tcgtaatggc agtcaatgat gcgatcgcac aaattgatgt ggaaactgag
    13081 aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt agttttaaac tgaaggagta
    13141 atgaatatgc aatatccaga accaattgcg aaactgatcg acagctacat gaaacttcca
    13201 ggaatcggtc agaaaacagc gacaagactc gccttttata cgatcgatat gaaagatgaa
    13261 gtagtaaatg aatttgctaa atctttgtta agtgtaaaac gcgatttgca tttttgtagt
    13321 atctgcggaa acatcacaga agaagatccc tgcgaaatat gtagaaatcc ttcacgtgat
    13381 cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa tggcaatgga aaaaatgcga
    13441 gaatatcacg gactttatca tgttctccat ggtgtgttat caccgatgga agggacaggt
    13501 ccagaagaca tcaatatttc ttctctgttg cagcggctgc atgatgcaga tgtaaaagaa
    13561 gtgattatcg caacaaatgc aacaacagag ggagaagcaa cagctatgta tctctcacgt
    13621 ctgatcaaac ccgcaggtat caaagtgact cgtctagcac atggtctgtc tgtaggctct
    13681 gatatcgagt atgcagatga agttacttta ttgaaagctg tcgaaggaag acgtgagtta
    13741 taaatttcta attaagtgtt ttttcagata caatataaaa gcagcacaag agatgggggc
    13801 aacaatgtga acggactatt tattacaatc gaggggcctg atggtgcagg aaaaaccagc
    13861 atcttgaatg aactttaccc tcttttgaaa gaagtagcaa aaacagaaat cattcaaaca
    13921 agagaaccag gaggcattcc gattgctgaa gagatccgag cagtgatcct tgatccaaaa
    13981 aatgatcgga tggatgaacg tacggaagca ctgctttatg cagcagcaag aagacaacat
    14041 ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa tcgttttatg cgatagattt
    14101 gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca tcggcgttac cgagattgct
    14161 cggttgaatg agtttgctac agaaggtact acgccggatt ttacccttta tcttgacgta
    14221 gattcagaca ccggattaag aagaatcaag aaaaacagac aaaatcaaat cgaccgttta
    14281 gattcggaag gtttggaatt ccaccagcgt gtacgtcatg cttacttaaa gctggcagaa
    14341 gagaatccgg aaagaatcca caaagttgat gcaagaaaaa gctttgaaga agtgctgcaa
    14401 acaagttatc atacaattat tgaacagtat ccgcaatttt ttgaaaacta ggaaggtgaa
    14461 cgtttatgaa aattatttta gcgattatcc aagacaaaga cagcaaccgt ttatcaaatg
    14521 aattgattga tgcaaatatc cgtgccacaa aactatcatc gacaggcggc ttccttaagg
    14581 ctggaaacag tacgtttatc gtgggtattg aagatgaccg tgtagaagaa gcattggaaa
    14641 tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc tacacctgtt acactggata
    14701 tttcgatgga tgggggcgtc ccttatccag tagaagtaga agtaggcggt gccacagtct
    14761 tcgtcttacc tgttgaagga ttccatcaat tttaggagga gctgagctat gaatcaagaa
    14821 ataaacttgg ctcaaatgca accgatcgtg tatcagcagc ttcaacggag ttttgagcat
    14881 gggcgtatcg ctcatgctta tctttttgaa ggggagaaag gaacaggaaa acatgaagta
    14941 ggtatatggc ttgcacagca cctgttctgt acccaaatga aagacaatct gccttgtgga
    15001 aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc cagatgtttt ggttattgaa
    15061 ccggaaggac aaaccatcaa agtcgatcag atcagacgtt tgcagacgga attcagtcgc
    15121 agcggttacg aatcaagaaa aaaagttttt ttgatcaaag aagctgaaaa aatgaatagc
    15181 agtgcggcaa acagtttgtt gaaattcttg gaagagcccc ctggagattt tttagcggtg
    15241 ttagaaacag atgcagtagg aagaattctc cctactattc aatctcgttg tcaagttctg
    15301 cattttcaag aactttcgaa agaagcactg atccatcgat tgcaagaaga gcagattcca
    15361 ttggaaaaag caaaactttt ggctttctta acaaacagtt taggaaaagc agttgaaatc
    15421 tctcaagatg aatggtttaa tgatgctaag gacttgattt atcaatggtt cgtttacttg
    15481 caaaagcaag atactcaagc ctttatctat gtccaaaaaa aattggtaaa atcatttaaa
    15541 gaaaaaacac aacagtttac aggattatct gtcttgatgt tttattatca ggaagcacag
    15601 cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac agataaacca gataattgaa
    15661 cggatattga tcgcagagca gaaactacgc agcaatgtca gttttcaggc tgttgcggaa
    15721 cagttcgttt tgcatacgat tttttagctt gccgcaaagg atgatgatca aggaggatgg
    15781 aaatggtaga agtagtaggt gtccgatata gagatgcagg tcatatctat tattttgcac
    15841 caggagactc tgaatatgtt tatagtgaca aggtattagt cgaatctcag caatccttac
    15901 atattgctac tgttgtcctt cctaaaaaag agattgatcc caatgacctt cctgaagcta
    15961 tccgtccgat cttgcataaa gcgtcacaag ctgaattaga aaaagacaaa aaaaacaaaa
    16021 aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt gcagttgcgt gagttggaaa
    16081 tgaaaatggt caaagccgaa tacacatttg atcgaaataa attgattttt tctttcacag
    16141 cggacgggcg aattgacttt agagaattgg taaaagatct ggccgctgta tttcgtactc
    16201 gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa gataataggc ggaattggtc
    16261 catgtgggcg tccattatgc tgttcttctt ttttaggcga tttcgtgcct gtatcaatca
    16321 aaatggcaaa agatcaaagt ttatctttga atccaacaaa aatttccggt ttatgcggac
    16381 gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga gacagcgaaa aaagaactcc
    16441 ctgactttgg aaaagtaatt gctacacctg atggtaaagg gaaagtcgta gggttgaatc
    16501 tgcttagccg tatcgttagt attcggctga ctggacggga aaatcctgtc gaatatgaat
    16561 gggatgaatt gaaagaattt atccaagtag gtgagatgaa tggataagag atcattatac
    16621 gacggattga atcaattaga atcagagttg agaaatacat taacagaact tgttgaaatg
    16681 aagcatgctt tgcatgagat cgtagaaaaa aatacaactt tggagatgga aaatcaacgt
    16741 ctgcgggaac acctgcggga attgaaccaa gttgcacaag ctccagctga aaatgtgaag
    16801 caggaacttt ctaagtctcg tatgaatctt gaaaaaattt atgaagaagg attccatgtc
    16861 tgctacgatt tatatggatc aagaagggac aatgacgagc cttgtgcgtt ttgtctagaa
    16921 gtgatctatc gcgaaagcgg tcgatgacca ataacaagag gagactggag cggagaaata
    16981 ctgctccagt ctttttatta aggagattgt gacagatgta taatcaaaaa agtttcaagg
    17041 gaaatccggt atatggggat ttatatcttg taccgacgcc aattggaaat ttagaagata
    17101 tgagctacag aagtgttcgt atgctgcaag aatccgatct tatcgcaagt gaggatacgc
    17161 gtaatacaca aaagttattg aatcattttg aaatccatac accccaaaaa agtttacatg
    17221 aacataatta taaagaacgt gtccctcaat tgattgcttt gttaaaagaa ggaaaaacga
    17281 ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga tcctggacat gagctagttt
    17341 tagcctgcat aaaagaaggg atttctgtga tctctatccc aggacctaca gctgggatga
    17401 cggctttgat tgcttcaggg ctagtgcctc aaccattttt attttatggc ttccttccac
    17461 gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa agaacagaca gttacgctga
    17521 ttttttatga gtcaccttat cggatcactg ctacattgac gaatttctta gaaatttttg
    17581 gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt ccatgaagaa taccttagag
    17641 gaagtacgga agagctactc gcttatatcg aagaacatcc agtaaagggt gaatgttgtc
    17701 tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga agttgatgaa gagttgggaa
    17761 cgttaaagga acaagtagaa gcaaagatcg cttcaggcga aaaaccaaat gctgctatca
    17821 aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt ctaccgtcag taccatgaat
    17881 tagactgaga tagtataagt tgtctataat gaaagactaa tgaggaaatc agctactaac
    17941 ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa tgaataagag gtgaggatga
    18001 tggagctgca atttccttta cataatgata catcaagaaa aatcattcat attgatatgg
    18061 atgctttttt cgcttcggtt gaagaacgag atcatcctga gtttcgcgga catccattag
    18121 taattgccag acaccccagt gacactggtg ggcgtggagt agtgactaca gcaaattatg
    18181 aagcaagaaa attcggaatt cattcagcaa tgagtgcaca aaaagcgtat gagctttgtc
    18241 cgcaagccat ctttaagcct ggcgaccatc ataaatacag cgatatttcc aaacaagtac
    18301 gggaagtatt ctatcgttat acagatattg tggaaccagt tagtatcgat gaagcgtatc
    18361 ttgatgtgac cgaaaataaa atcaattgca agtcagccat caaaatcgcc cgaatgatcc
    18421 aagcagatat ttgggagagt gtccagctaa catgttctgc gggagtaagt tacaataagt
    18481 ttttggccaa attagcatca gactatcaaa aacctagagg aatcacggta gttgcccctc
    18541 aagacgctgt gccattttta aaagctttgc caattgaaaa atttcatggg gtagggaaga
    18601 aaactgtacc tagaatgcat gagctaggga tttatacagg aaaagaccta tatgaatgca
    18661 cagaaatgat gttgattcga aattttggaa aaatgggcta ttctctttat cgtaaagtca
    18721 gaggaatcca tgattctcca gtaaacgtta caagagagcg aaaatctgtg ggaaaagagc
    18781 atacttatgg gcagccgttg caaacggaag aagcagtgct gactcagttg cgccaactag
    18841 ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca tgggaaaaca gtagttttga
    18901 aggtcaggta cacggactat tctactgtga ccaaaagagt gacattgcca gaatatatct
    18961 ataaaaaaga agcactcttt tatcaagcaa gccttatatg ggaggaaatc ttgggagtag
    19021 aaaaaggaat ccgattgtta gggattactt tgacgaatct ggacccgatg acttatgaaa
    19081 acatcgtcct tccattatgg gaaaatcaag aaatataaaa acagctttga gagttgaaac
    19141 cggaaatccg ttttctctcc caaagctgtt tttatatcaa gtcttgcttt ttgatttgtt
    19201 cataggtttc tgttgcatcc gtacattttt cccaaatcac aacttgattg gattctaaag
    19261 atttttggac gtcaatgatc cgctggttgc tacttccgcg aaattgtagt tttaggtcac
    19321 gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat ttgcgaaagg agttctaatt
    19381 tatcttctga atcttgtagc aattcatcaa aggtatagcc tgtccatgac cagatatctt
    19441 ttgtcgtacc gaactctttt cggatacgtc gtacgacttg cagacaaaca tctgtattca
    19501 aaaaaggttc gccaccaagt aaagttaatc cttgcacgta atcgttgcgc aagtcagcga
    19561 tgatttgttc ttctaattca ggtgtgaatg gtttgccata tcggaaattt tgtgcagctt
    19621 tgttgaagca gccttcgcag gcaaataagc agccactaac gtaaagactg ttgcggacac
    19681 cttcaccatc gacaaaatta aacggtttgt agtcagcaat atagttttgg ctgtagtctt
    19741 ttgctaacca ttcttttggt tttggatttc tcataaaaaa acttctttca tttaaggttc
    19801 aaagatatag ctattgaaga taaaatctac tcgaccacat aaataagaac aaactgacct
    19861 aaatccattg ttcaagaaag cttgttctta ttttgataga gggcgttcaa agatacaaag
    19921 ccatcgaaag agggtggtga aaaaagactt gtcatagcct cttattctga ataaacggtg
    19981 ttcaaaagct tgcatctgca gtaataagcc caactaaacg aaaaatatga ggaactattt
    20041 ttgtttagct attcttatta cttgatgcag gacagctttt tcacaacctc atttcatgtg
    20101 tttcacacgt gaggaaattt ctttatggcg gccatgtacc atcggacgag cttgcggatt
    20161 tcctaaatag ccacaagtac gtttgactac atcacatgtt tttggatcat gatttccaca
    20221 ttctggacat tcgaaccctc gttctgtcgg tttgaaatca ccttcgaaac cacattgata
    20281 gcaatgatcg attggcgtat tcgtaccaag atacccgatt ttgtcatatg caaaatccca
    20341 tacagcttct aaagctttcg gattttgttg gagcataggg tattcacagt agtggatgaa
    20401 tccacctgaa caatattttg gataatcctt ctcaaattcc aatttttcaa atggcgttgg
    20461 gtttttacgc acatcatagt ggaaactgtt tgtataatat tctttatctg tgatattttc
    20521 gatgatgccg aatttttctg tatccaaacg gcagaaacgg tcagttagac tttcacttgg
    20581 agttgaatag acactgaaat gataaccata ttcatccccc catgcatctg cattagcttt
    20641 taattctttg acgatatcaa gtgtgaaagc ttttgcttct gggtttgttt cccattcgcc
    20701 accgaagaaa gctgaagcaa cttcatacaa tccaatgtag cctaacgata cagtagctcg
    20761 acggtttttg aataattcat tgacagaatc ggtacgtgaa agacgtttcc cgaaagcacc
    20821 atacatatat aaaattggcg catttgctgg aatggcttct ttacaacgtt ctacacgata
    20881 gagaagcgca tctttcatga tttctaagcg gtcagacagt aactgccaga atttattctg
    20941 atctcctttt gattcgatcg cgattcttgg caaattcaat gtaacaacac ctaaattcat
    21001 tcgtccgaca tttacttctt gaccattttc gtctttccac ccttgcaaga atgaacgaca
    21061 gcccataggt actttgaaac ttccagttaa ttcaacgatt ttatcgtaat tcagaatatc
    21121 aggatacatt cttttcgttg cacattccaa tgctagttgt ttgatgtcgt aattaggatc
    21181 ttcagggttt aagttcacac cttttttcaa agtgaagatc agtttaggga agatagctgt
    21241 ccgcttttca ctccctaatc cattgattcg aatctgtaaa attgcttttt gaatttctct
    21301 ttcaaaccag ttttctccta gtccaaatcc taatgaagtg aaaggagttt gtccatttga
    21361 agtaaataag gtattgattt catattccaa actttgcata gcatcgaaga tatctttttt
    21421 ggtttttgct tttgcatatt ctttttggcg ttctggcgaa tcgatccatt cttccgcgtc
    21481 ttttagatgt tttttatagt tcaattctgc aaaaggagca agcaattcat ctgttcggtc
    21541 ggcagaacag ccaccatatt gacttgacgc tacattggca ataatctgtg agatttgtgc
    21601 tgtggcggtc tggatagatt ttggcgattc gacttcggca ttgccgattt tgaacccatt
    21661 gttcagcatg cctttgaaat caatcaagca gcaattcgtc atcggtgtat aaggatgata
    21721 atccaaatca tggtaatgga tatcgccttt ttgatgcgca tttgctatat gtggtggcaa
    21781 cattttcagt ccgattgatt ttccaacgat cccggctgtc agatctcgct gtgtattgaa
    21841 aacattactg tctttatttg cgttttcgtt cacgacagtt tggtccttgt tgattagctt
    21901 ttcaatcgaa aagttgatat ctgttgcttt actgcgagca aaatctcgtt tggtacgata
    21961 attgatgtac tcttctgcta attcatattc gtttttttct agtaatatat gttccacgat
    22021 gttttgaatc tcatagattt tgacattttc agcaaaccgg cttttgattt cttggacaac
    22081 caagtctgca atcatttgaa ttttttcata agtaagtggt gataatgagc cgtgaatttt
    22141 cttttctgct ttgatcagtg cttcattgat cttttgttca tcgaaatcaa catggcggcc
    22201 atcgcgtttg atgactttca tctgggatgt ctctggctgc tgagtaacga cagttttttc
    22261 ttcataattt actttcatta tcttcaactc ctgctaaatt gttcttgtct atcataaaac
    22321 aaaatcgggg aaggagcaac taaatatcgg ctatatatag tattcaatgt gctgtataat
    22381 tggatttcta tactatatgt agtgtatatt ttattgtgaa aaaaggtgaa aacttgtagc
    22441 tggtggattt gttttttgca tatttaattt tgctgaataa aataattgaa taaaattttt
    22501 tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa ttattcgtag atatgctact
    22561 atgcaaataa atagaaatga aaaatgaaag aggaggagaa gaattggaaa agatgatcga
    22621 agtgaaacat ctaagtaaaa gctacggcag caaactttct cccgtcaaag ccttagataa
    22681 tatctcattc acagtagaag aaggcgagtt tgttgggatt atgggcccaa gtggtgcagg
    22741 aaagacaacg ctgatgaata tcttgtccac gatcaatttg cctacgatgg gcagtgtctc
    22801 gatccaagga aaagaaatca caaaaatgaa aaacagtgag ctcagtgact ttcgtcgaaa
    22861 aaaactagga ttcatttttc aagagttcaa tttgatcgat accctaaatg caaaagacaa
    22921 tatcctattg cccctagcag ttgaacggat gactaaagag gaaatggaaa agagagtaag
    22981 acatgtagct caattgttga atatagaaga actgctcaaa cggtatcccg atgaactttc
    23041 tgtcggacag agacaacgaa ttgcagctgc tagagcactt gttgttcagc cacaagtgat
    23101 ttttgcagat gaaccaactg gctctttgga ctcgaagtcg gctacagaat tgttgaacta
    23161 cttgaagacc atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc atgatccata
    23221 tacagcaagc tactgcgata gaattttatt tattaaagat ggtgtgattt tttctgaagt
    23281 tgtcagaaga gggacaagaa gagagttctt tgagaaagta atagacatgc aggcgacgat
    23341 tggcggagga ggaaaaatga atgctgtata aactagccgt taaaagtttt tttcggcagt
    23401 cacgaggcta tcttgtttat tttttcagtt tgactttatc cgtaatgatc tattattcct
    23461 tcagcgcaat gacctacgat caatctttag tcagaagagc aagtcaagat acttcgatag
    23521 atggtggatt gagttttggt ggatttatca ttactgttgt tttgcttttt ttcgtattca
    23581 gtgccaaccg atttttcttg aatagaaggc aaaaggaaat aggcatctat cagctttttg
    23641 gtatgaataa actgcagata tcaggtatat atgtgttaga aatcatgata attggattgt
    23701 tcgcctgtat ttctgggata ttactcggta ttattttttc caaactcttt tcaatgattt
    23761 tggttcgaat gatggatatg gatcttacta gtccattttt tatctccatt ccatcagtag
    23821 tagatacttt atttgctttt ttcatcatcc ttttagcagt atctgtatat tcaatatgga
    23881 aaatatggcg ctatccaatg attcgaagtt ttggagaaaa agaacaagca gatagttcaa
    23941 caatgcgctt caggacaagg caccgattac tcgcagtcat cgggttacta cttttaacga
    24001 gcggatattt tggagctagt cattttaggg aaatcacttc atggatgatc agttatcatt
    24061 atcttgatac attagttttc ttttttcctt ttttaatttt tttcgtttgt gtggtcggta
    24121 cttatttgtt tttctgttat acatttcggt tatttttgat catattgagt aaatggaaga
    24181 tttattacta tggtatcaat tttttgataa tgggtaacac gcaagttcat ttgctcaaaa
    24241 gttggcgtac aaattctctg attacagtca ttctcggaat ttctttaatg acaattggcg
    24301 gcacggctag tatctctact attttatcct accaggagaa agtttcctcc ccgatggact
    24361 atcagtttga tgttgagaca gagaaaaaaa tcaagccgat tctagcagag gaagggcaga
    24421 agataactaa agaaatcacc ttgcattata aagtcattgg gagtatctat agtcaaaatc
    24481 tcgaagaacc tatgtttcaa actgctaatc tgatcacgga aacacaatat caagccttca
    24541 gaaaagttaa tccagatttg ccgaacattt ctttgaaatc acctggtcat actgttttgc
    24601 ttgatcaaat gcaaactctt tttcggaata tttcattata tggatctagt attggactgc
    24661 cagataatca agaattgaag attcaacaaa tgcttccaag tgtattaggt gacgaaacaa
    24721 tggtatatct aagtcctgta ctgattgtag atgataccgt ttttgaaaaa gcaaaaggtg
    24781 tggattatca agtagtcaat gtagatgtat caggtggaaa taatgaaaaa atagatgaac
    24841 gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt ttattattct tatgatatcg
    24901 taaatggaag tctacaagga gaaattagca caaagaaact tccggatgac caaatgaagg
    24961 aagcacaatt tagccaagaa agctctcgtt tgaatttctc ttccagctat tcgaaattgc
    25021 gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac actatttgtc gggatgatcg
    25081 tgattattgc tactggtagt attttgacag tccggcaatt agcagaagca gaggaagaaa
    25141 aagaaaatta tcacctattg actaagctgg ggattccaca aagaaggatc agacggatga
    25201 tcttcgagca aaacgcactt accttctttc cacccatgat attagggatc actcacgcag
    25261 tatttgcaat caatgtcttc actcagtata taaaaactgc cgattactgg ttggcttatc
    25321 ttgtgagtgg cttattgatc cttatctatc ttttgcttta tgtcattacg agtcagttat
    25381 attgtcggat tatcgaggaa tgatatacgg aataagacaa gataataaat agaactaaat
    25441 tttgaatacc atttatttgg aataagaggt tgtgacacga tttttgtcac aacctcttta
    25501 ttttttatat tttttaaaag tacgagaaat tataaagaaa aaagcagtga tttgatgtgc
    25561 tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct gtaagctaga
    25621 cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg aggagggaat
    25681 ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt catcattcga
    25741 acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta taaaaatatt
    25801 caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa tcaaaacgat
    25861 tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt atcaggcgga
    25921 tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt attatttaga
    25981 aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt ttgaaatgat
    26041 tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg aagagcatac
    26101 acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg tcatcagtat
    26161 cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag aatttcaaga
    26221 aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta cagaaaattt
    26281 ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga
    26341 taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc ctggtatgca
    26401 tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt atcctgcatt
    26461 agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact ttcgtcaatt
    26521 attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat catttatgga
    26581 tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg acatgttttt
    26641 attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa
    26701 tgcacggata tataatactt acggtccaac agaagcaact gtagctattt caagtattca
    26761 gattgaccag catgtactag atagttatga ccgactaccg attggctacg taaaaaaaga
    26821 cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat
    26881 cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa agacagctgc
    26941 tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag gtagactaga
    27001 tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat tacatggata
    27061 ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc
    27121 tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt
    27181 agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag aacaattact
    27241 agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc tgctgcctca
    27301 gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg
    27361 ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc cattgcgctt
    27421 gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta tcaatcagca
    27481 gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca gggagcggcg
    27541 ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt ttatcggcaa
    27601 aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat tccactgttt
    27661 ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg ttttttagga
    27721 atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga tggcatgatc
    27781 aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac aatctcttct
    27841 ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc tgttcgtgat
    27901 aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt tttatataaa
    27961 ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg cattgcctta
    28021 cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag tatgtatttg
    28081 ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat gatgggatat
    28141 cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga attctggaat
    28201 cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg attgatgttc
    28261 ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt
    28321 gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat cgtatatgga
    28381 ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa gaagaagcat
    28441 aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt gacatttcaa
    28501 gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt ttttagctaa
    28561 cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa gaacaaattt
    28621 tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt gatattgatc
    28681 tatttgatga agggttgctt gattctttag catctgttca attattagtc gagctagacg
    28741 gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg agcacaccga
    28801 ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga aactttttgc
    28861 tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta cctcctccta
    28921 tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa
    28981 cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat atattccttt
    29041 ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc tggcagaaaa
    29101 ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat cgttaagcca
    29161 atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg tctttattat
    29221 ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata cgtattattc
    29281 tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta
    29341 tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt tgacggcgat
    29401 tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc agctgcgcag
    29461 ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt tgaaagatcg
    29521 acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag aaacagaatt
    29581 atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat tcgggttgaa
    29641 aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac agtcacaaaa
    29701 aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct tggatcaatt
    29761 agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga aatggagcga
    29821 ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca aatttcaatt
    29881 aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta ccaactattt
    29941 tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc aacagatcag
    30001 accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg atgctttttt
    30061 tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa gaaaacaggc
    30121 gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca
    30181 agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg
    30241 ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga
    30301 gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct
    30361 gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc
    30421 cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc
    30481 gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct
    30541 cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa
    30601 ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc
    30661 attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat
    30721 atggtcactc catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa
    30781 gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa
    30841 gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga
    30901 ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt
    30961 acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc
    31021 ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt
    31081 attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga
    31141 actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt
    31201 cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca
    31261 gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg
    31321 agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt
    31381 ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga
    31441 gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc
    31501 acttattgag ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt
    31561 acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt
    31621 tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact
    31681 ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata
    31741 tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg
    31801 atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta
    31861 ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg
    31921 ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt
    31981 tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc
    32041 ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga
    32101 ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa
    32161 aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt
    32221 taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat
    32281 ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac
    32341 accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg
    32401 ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat
    32461 tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa
    32521 atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga
    32581 ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa
    32641 caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt
    32701 ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc
    32761 atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga
    32821 aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc
    32881 aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca
    32941 tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta
    33001 gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat
    33061 atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga
    33121 gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc
    33181 taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc
    33241 cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct
    33301 ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac
    33361 gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat
    33421 atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc
    33481 aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa
    33541 atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta
    33601 gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct
    33661 gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct
    33721 cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg
    33781 atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa
    33841 aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt
    33901 gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga
    33961 aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg
    34021 taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt
    34081 gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca
    34141 tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg
    34201 caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt
    34261 ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga
    34321 gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga
    34381 atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg
    34441 ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag
    34501 tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca
    34561 aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc
    34621 tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt
    34681 aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc
    34741 gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa
    34801 tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta
    34861 tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta
    34921 gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg
    34981 ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt
    35041 cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa
    35101 aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca
    35161 aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt
    35221 ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc
    35281 atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt
    35341 cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg
    35401 gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga
    35461 ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta
    35521 atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc
    35581 gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg
    35641 tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc
    35701 atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa
    35761 aataacaatg ataatataat gattatgctc cactttatac gtaaataaaa agattctttt
    35821 ttattatata tgttattaaa aaaagacata aaggtattcc tttctaaaac ttgtaatgtt
    35881 agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag ataaagatga
    35941 ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc tataatttga
//
LOCUS       DACCQP010000022        34788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCQP010000022 DACCQP010000000
VERSION     DACCQP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.022"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(233..1081)
                     /locus_tag="IVO87_001224"
     CDS             complement(233..1081)
                     /locus_tag="IVO87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7449430.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVO87_001225"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVO87_001225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7449431.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IVO87_001226"
     CDS             complement(3582..3761)
                     /locus_tag="IVO87_001226"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7449432.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVO87_001227"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVO87_001227"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7449433.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IVO87_001228"
     CDS             5909..8188
                     /locus_tag="IVO87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7449434.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IVO87_001229"
     CDS             complement(8281..8928)
                     /locus_tag="IVO87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449435.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IVO87_001230"
     CDS             complement(9107..9826)
                     /locus_tag="IVO87_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7449436.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IVO87_001231"
     CDS             complement(9957..11108)
                     /locus_tag="IVO87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7449437.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IVO87_001232"
     CDS             complement(11325..12065)
                     /locus_tag="IVO87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7449438.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVO87_001233"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVO87_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7449439.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IVO87_001234"
     CDS             complement(12449..13042)
                     /locus_tag="IVO87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7449440.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IVO87_001235"
     CDS             complement(13026..13673)
                     /locus_tag="IVO87_001235"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7449441.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVO87_001236"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVO87_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7449442.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVO87_001237"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVO87_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7449443.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IVO87_001238"
     CDS             complement(15119..15646)
                     /locus_tag="IVO87_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7449444.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IVO87_001239"
     CDS             complement(15826..16611)
                     /locus_tag="IVO87_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7449445.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IVO87_001240"
     CDS             complement(16604..17473)
                     /locus_tag="IVO87_001240"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7449446.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IVO87_001241"
     CDS             complement(17474..18853)
                     /locus_tag="IVO87_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7449447.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVO87_001242"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVO87_001242"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7449448.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVO87_001243"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVO87_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7449449.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVO87_001244"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVO87_001244"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7449450.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVO87_001245"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVO87_001245"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7449451.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVO87_001246"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVO87_001246"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7449452.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IVO87_001247"
     CDS             complement(22811..23035)
                     /locus_tag="IVO87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7449453.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IVO87_001248"
     CDS             complement(23088..24050)
                     /locus_tag="IVO87_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7449454.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IVO87_001249"
     CDS             complement(24060..24500)
                     /locus_tag="IVO87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7449455.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IVO87_001250"
     CDS             24806..25771
                     /locus_tag="IVO87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7449456.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IVO87_001251"
     CDS             complement(25828..26058)
                     /locus_tag="IVO87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7449457.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IVO87_001252"
     CDS             complement(26144..27031)
                     /locus_tag="IVO87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7449458.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IVO87_001253"
     CDS             complement(27120..28343)
                     /locus_tag="IVO87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7449459.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IVO87_001254"
     CDS             complement(28360..29439)
                     /locus_tag="IVO87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7449460.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVO87_001255"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVO87_001255"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7449461.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IVO87_001256"
     CDS             complement(31297..31563)
                     /locus_tag="IVO87_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7449462.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IVO87_001257"
     CDS             32042..34276
                     /locus_tag="IVO87_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7449463.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IVO87_001258"
     CDS             34424..34744
                     /locus_tag="IVO87_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7449464.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9842 a         7509 c         6064 g        11373 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaa
//
LOCUS       DACCQP010000023        34695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCQP010000023 DACCQP010000000
VERSION     DACCQP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.023"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IVO87_001259"
     CDS             591..1280
                     /locus_tag="IVO87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449465.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVO87_001260"
     CDS             1267..2475
                     /locus_tag="IVO87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7449466.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IVO87_001261"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IVO87_001261"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7449467.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVO87_001262"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVO87_001262"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7449468.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IVO87_001263"
     CDS             complement(6578..7273)
                     /locus_tag="IVO87_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7449469.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVO87_001264"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVO87_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7449470.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IVO87_001265"
     CDS             complement(8472..8663)
                     /locus_tag="IVO87_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7449471.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IVO87_001266"
     CDS             complement(8684..9574)
                     /locus_tag="IVO87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7449472.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IVO87_001267"
     CDS             complement(9561..10328)
                     /locus_tag="IVO87_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7449473.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVO87_001268"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVO87_001268"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7449474.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVO87_001269"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVO87_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7449475.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVO87_001270"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVO87_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7449476.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IVO87_001271"
     CDS             14944..15282
                     /locus_tag="IVO87_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449477.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IVO87_001272"
     CDS             complement(15450..16208)
                     /locus_tag="IVO87_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7449478.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IVO87_001273"
     CDS             complement(16226..17044)
                     /locus_tag="IVO87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7449479.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVO87_001274"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVO87_001274"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7449480.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVO87_001275"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVO87_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7449481.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IVO87_001276"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IVO87_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7449482.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IVO87_001277"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IVO87_001277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7449483.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IVO87_001278"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IVO87_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7449484.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IVO87_001279"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IVO87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7449485.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IVO87_001280"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IVO87_001280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7449486.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IVO87_001281"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IVO87_001281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7449487.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IVO87_001282"
     CDS             27086..27385
                     /locus_tag="IVO87_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7449488.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IVO87_001283"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IVO87_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7449489.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IVO87_001284"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IVO87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7449490.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IVO87_001285"
     CDS             28352..30325
                     /locus_tag="IVO87_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP7449491.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDNKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IVO87_001286"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IVO87_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7449492.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IVO87_001287"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IVO87_001287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7449493.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33198..34490
                     /locus_tag="IVO87_001288"
     CDS             33198..34490
                     /locus_tag="IVO87_001288"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7449494.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10570 a         6746 c         6860 g        10519 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagataa
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaatc
    33121 attcgatttt atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata
    33181 tagaacgagg tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag
    33241 ggaaaggaaa aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag
    33301 gtggcgacaa tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga
    33361 ttccttctgg gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca
    33421 atcctaaatc attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta
    33481 atttgcgtat ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat
    33541 tacaagaaga tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg
    33601 cctatatgga taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa
    33661 tttttgaaga acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt
    33721 ttgattcaga accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac
    33781 aaatcaaaca atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca
    33841 aacgcgtgtt gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc
    33901 cttttgttac ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc
    33961 cttcaaaaat caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg
    34021 gaccatttcc aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag
    34081 aatacggtac gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc
    34141 gccattctaa acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa
    34201 gtggattgga aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc
    34261 actatccagc aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag
    34321 gctggtcaga agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta
    34381 actatgtacg tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac
    34441 ctgatagaac acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac
    34501 gacgactgaa tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata
    34561 ataacttgaa gaggtagtga aagagacgtt tagccttatt gccgataggc tgtctcttga
    34621 acactgttta ttcagaataa gggactgtga caaaaaccgt gtcacagtcc ctttttccga
    34681 acaaacggtg ttcaa
//
LOCUS       DACCQP010000024        31424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCQP010000024 DACCQP010000000
VERSION     DACCQP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.024"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            300..620
                     /locus_tag="IVO87_001289"
     CDS             300..620
                     /locus_tag="IVO87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7449495.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            774..1679
                     /locus_tag="IVO87_001290"
     CDS             774..1679
                     /locus_tag="IVO87_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449496.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1676..2449
                     /locus_tag="IVO87_001291"
     CDS             1676..2449
                     /locus_tag="IVO87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7449497.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2630..4054)
                     /locus_tag="IVO87_001292"
     CDS             complement(2630..4054)
                     /locus_tag="IVO87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449498.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4265..4711
                     /gene="nrdI"
                     /locus_tag="IVO87_001293"
     CDS             4265..4711
                     /gene="nrdI"
                     /locus_tag="IVO87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7449499.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4776..6758)
                     /locus_tag="IVO87_001294"
     CDS             complement(4776..6758)
                     /locus_tag="IVO87_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449500.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6772..7668)
                     /gene="murQ"
                     /locus_tag="IVO87_001295"
     CDS             complement(6772..7668)
                     /gene="murQ"
                     /locus_tag="IVO87_001295"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7449501.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7671..8732)
                     /locus_tag="IVO87_001296"
     CDS             complement(7671..8732)
                     /locus_tag="IVO87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7449502.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8943..9470)
                     /locus_tag="IVO87_001297"
     CDS             complement(8943..9470)
                     /locus_tag="IVO87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7449503.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9537..11039)
                     /gene="lsa"
                     /locus_tag="IVO87_001298"
     CDS             complement(9537..11039)
                     /gene="lsa"
                     /locus_tag="IVO87_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7449504.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11297..12418)
                     /locus_tag="IVO87_001299"
     CDS             complement(11297..12418)
                     /locus_tag="IVO87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7449505.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12543..13484
                     /locus_tag="IVO87_001300"
     CDS             12543..13484
                     /locus_tag="IVO87_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7449506.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13634..14530)
                     /locus_tag="IVO87_001301"
     CDS             complement(13634..14530)
                     /locus_tag="IVO87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7449507.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14799..16139
                     /locus_tag="IVO87_001302"
     CDS             14799..16139
                     /locus_tag="IVO87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449508.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16357..16800
                     /locus_tag="IVO87_001303"
     CDS             16357..16800
                     /locus_tag="IVO87_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7449509.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17065..18483)
                     /gene="nhaC"
                     /locus_tag="IVO87_001304"
     CDS             complement(17065..18483)
                     /gene="nhaC"
                     /locus_tag="IVO87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7449510.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(18886..20109)
                     /locus_tag="IVO87_001305"
     CDS             complement(18886..20109)
                     /locus_tag="IVO87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7449511.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20140..20979)
                     /locus_tag="IVO87_001306"
     CDS             complement(20140..20979)
                     /locus_tag="IVO87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7449512.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(20996..21676)
                     /locus_tag="IVO87_001307"
     CDS             complement(20996..21676)
                     /locus_tag="IVO87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449513.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21678..22751)
                     /locus_tag="IVO87_001308"
     CDS             complement(21678..22751)
                     /locus_tag="IVO87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449514.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(23397..24068)
                     /locus_tag="IVO87_001309"
     CDS             complement(23397..24068)
                     /locus_tag="IVO87_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7449515.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(24155..26833)
                     /gene="mgtA"
                     /locus_tag="IVO87_001310"
     CDS             complement(24155..26833)
                     /gene="mgtA"
                     /locus_tag="IVO87_001310"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7449516.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVDGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            27492..27929
                     /gene="ndk"
                     /locus_tag="IVO87_001311"
     CDS             27492..27929
                     /gene="ndk"
                     /locus_tag="IVO87_001311"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7449517.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(27951..28817)
                     /locus_tag="IVO87_001312"
     CDS             complement(27951..28817)
                     /locus_tag="IVO87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7449518.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(28977..31070)
                     /locus_tag="IVO87_001313"
     CDS             complement(28977..31070)
                     /locus_tag="IVO87_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7449519.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         9111 a         6557 c         5555 g        10201 t
ORIGIN      
        1 aatatataga aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc
       61 tttctatgaa attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct
      121 tggtacactt tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca
      181 gaaaaagata tacgatctgc caccgggtag gataaagagg atgatcattc cattaggtct
      241 tttaagggat gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta
      301 tggcatggat ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga
      361 aatggagtga tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg
      421 ctagttttta ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta
      481 tctggacagg gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata
      541 agctgaatct ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca
      601 agattactag cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt
      661 tatttaatat tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt
      721 ctccactcat caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa
      781 gtctttcggt ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca
      841 gttttagctt atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa
      901 caacgatcat gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg
      961 gtgaaaatga gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg
     1021 aatacccggg tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa
     1081 ccaacgatgt atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc
     1141 acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct
     1201 tactgaacca tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag
     1261 caacaagaga tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga
     1321 tctccagtca cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc
     1381 aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca
     1441 tcgttatctc tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata
     1501 ctttggaaga tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg
     1561 tgatccagct attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg
     1621 atttggaatc ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac
     1681 attgcttgta aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc
     1741 tgttctatta gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat
     1801 cattgatcca gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat
     1861 catgattgca gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa
     1921 tctcctttat cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt
     1981 tatttattct gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat
     2041 tcttttccct tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag
     2101 cttgttcact tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt
     2161 gatgattgct tgctttatca atagttccgg tgcatccatc tcagcaggaa tcgtctttta
     2221 ttttgcttcg tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat
     2281 caaatggaat ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat
     2341 gaagcaattg actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt
     2401 tttgttctta gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa
     2461 aaaagaggct gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg
     2521 tttattctga aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt
     2581 ttgaataaac gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa
     2641 gcatcccagt ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta
     2701 aaatcttatc taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt
     2761 aaaaataaat gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga
     2821 ctagcatagc ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt
     2881 ctttccgctg ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa
     2941 tagctgctgt accaagtgtg taagcaaagt tataagcact atcagtgaat aataatgaaa
     3001 gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt
     3061 tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt
     3121 caatcggtaa gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt
     3181 tgatcacgac tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct
     3241 gcgccttgat cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca
     3301 agacgtaaat aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt
     3361 gtttttttgc tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca
     3421 tcaccatgat gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca
     3481 atataatgtc gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta
     3541 ctacaataaa taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac
     3601 tagcattttc gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca
     3661 tcacgatcga tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc
     3721 tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac
     3781 cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt
     3841 ttaaatctgg acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg
     3901 ttcctacgag tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca
     3961 taataccaaa tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta
     4021 aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc
     4081 ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa aactttaata
     4141 gaaatataat tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca
     4201 aattacacca ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg
     4261 atccatgaac attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct
     4321 agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa
     4381 agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc
     4441 tacttattta gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac
     4501 gatgcgtgaa tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag
     4561 cggaaataaa aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt
     4621 tggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat
     4681 ttatcagatt ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt
     4741 cagtttcttc tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt
     4801 gcttttgctt ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg
     4861 gttaccaaga cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct
     4921 tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc
     4981 gtaccgccta agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc
     5041 gttgtgatcc caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga
     5101 gaatagatat gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca
     5161 aaaactggat cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg
     5221 atagtcacac tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg
     5281 gctgtttcag tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat
     5341 gtagcaacga agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta
     5401 ccgtcagaga tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca
     5461 tttccgatca ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat
     5521 gggcgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg
     5581 actggtaaag ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat
     5641 aatgccaatg ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc
     5701 gtacgaccag tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg
     5761 aacataacca tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg
     5821 ccaagtaccc agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg
     5881 atgaagaaaa tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata
     5941 tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca
     6001 gcgaagatga cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag
     6061 tttttcagtg gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca
     6121 ccaagagttg gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg
     6181 tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct
     6241 gcatcaagtg ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc
     6301 ccagtaccga caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc
     6361 gatttgattt tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt
     6421 tcgtttacac ggtcttttcc agatgctgat gaatttgctg tagctgcact tacagaagaa
     6481 agtttttctc ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact
     6541 ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga
     6601 atcgctttta attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga
     6661 gtcatgcagt ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt
     6721 tttgccagtt cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt
     6781 cccttttaca aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt
     6841 tgtcaaaatc atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc
     6901 agctgtttcg taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag
     6961 tttttcattt gttggtttga catcgaccat caagttattg aatactttac ctataccaat
     7021 catactgata gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg
     7081 agtagagcct gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt
     7141 actgatttcg gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc
     7201 gtaatctaat ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat
     7261 atcatttttt gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc
     7321 ggccccttcg acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat
     7381 ttccggttct acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc
     7441 acttgttccg gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt
     7501 agcaatcact ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc
     7561 ttctttgttc attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc
     7621 ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct
     7681 tcttttctta taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct
     7741 tctttgataa ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt
     7801 aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc
     7861 atacgcgtag ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctgcg
     7921 cttcgtatga catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg
     7981 taaaattctt catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa
     8041 gcaaattgct cctgaacatt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg
     8101 ttacttgcta atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa
     8161 gttggcaatc cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg
     8221 acaaggagac cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc
     8281 cctgtttcgg gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct
     8341 gcttctttca attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc
     8401 aattgatgtg accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct
     8461 gttacaccaa ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat
     8521 gcctctcctg aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt
     8581 tcgatcaatc gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa
     8641 ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa
     8701 gaatcgttca taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt
     8761 catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata
     8821 attaaatttc atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa
     8881 aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat
     8941 aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta
     9001 ggcatcggat gaaaataagc attgatcact cttatcaagg cattatgaca aactaacaga
     9061 tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca
     9121 tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag
     9181 ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca
     9241 tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt
     9301 tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct
     9361 agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact
     9421 aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct
     9481 ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag
     9541 attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc
     9601 actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag
     9661 acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat
     9721 ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa
     9781 acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg
     9841 tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg gcgtacataa
     9901 ctaatgctca gatgttttgg ctgggatttt tctcctataa ccttgccgtt gaatgccccc
     9961 agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc
    10021 tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga
    10081 agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa
    10141 tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga
    10201 ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta
    10261 ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct
    10321 cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact gacttctttt
    10381 ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag
    10441 atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc
    10501 actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt
    10561 ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg
    10621 attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct
    10681 ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg
    10741 agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt
    10801 aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact
    10861 tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg
    10921 tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat
    10981 aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt
    11041 ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa
    11101 gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct
    11161 gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt
    11221 aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag
    11281 aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta
    11341 agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac
    11401 ccaaatgaat acttaagaca attgggagaa aatcagagag tccttctagc tcttggtctc
    11461 tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt
    11521 gtggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat
    11581 ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa
    11641 ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg
    11701 gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt
    11761 gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag
    11821 cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt
    11881 tagaaacaag aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc
    11941 cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc
    12001 tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt
    12061 tgataagttc tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat
    12121 caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtattt tttccttgcc
    12181 aagttgtcaa taaatagctg atttttcgct gacggaataa aagatccatc ccatcaatgg
    12241 tacgccatgt tcccccgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg
    12301 caatcggcac attgattttt cttcctttac gatcttccca gccatttaga actgcctgat
    12361 ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct
    12421 gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat
    12481 gctattttct tccatttttc tatatactaa aagggaactc attgataaag gagagaaaaa
    12541 acatgaaaat cgcaatagct ggagccggag caatgggcag ccgaatcgga ttgatgctac
    12601 atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgcacacatt gaagccattc
    12661 gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct
    12721 attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa
    12781 aagcgaatca attagatgat atgtttcgtt cgatccaatc cattattact gacaatactt
    12841 atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa
    12901 agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag
    12961 tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat
    13021 ttacaaaaaa agttgtggaa gtctttcaag aagcaaaatt aaatccgatc tatagccata
    13081 atgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca
    13141 cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa aacggcagag agcatggttc
    13201 gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata ttagatcaag
    13261 aagaagtgta ccaacatatt gcttctactt ataatcctga caacatcggg ttgcattacc
    13321 catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg
    13381 gtgccgtttg gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga
    13441 cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag
    13501 aatcagctga atcagcagct ggttctcttt ttttaagccc aatgaaagat caaaaagaaa
    13561 acacaactct catgatgcca taagaattgt gcttgtttgt ttcggtacat aatctcttca
    13621 aaatgtaggt tttttattta ttttcaacag aggagaggtc atattcgtat tgctcataaa
    13681 tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata
    13741 gctctgatgg gattggctta aaaggtgtat gcagaaattc cggtaattca tctaaatcac
    13801 gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat
    13861 tatccgtcac tagtttcttt tgaaaataca tagattcaaa tgtccgcaaa gtcactcctt
    13921 gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact
    13981 cttcataagc aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg
    14041 tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat
    14101 tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa tccatatgtt
    14161 tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat
    14221 gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg
    14281 ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc
    14341 gagccatctc catattaaat ccataatcta aaaaaacgat cagctgataa tttgccaact
    14401 gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta
    14461 aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat
    14521 ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa
    14581 atagtaaaaa gccagtaaaa cgcttcctat atttgtatga aaatttaaca tttgtatgaa
    14641 aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg
    14701 taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata
    14761 taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa
    14821 aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc
    14881 tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt
    14941 tgattggcgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg
    15001 gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg
    15061 ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg
    15121 ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt
    15181 ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa
    15241 cagaatattg gctggctttt gctaaaatca gtgtcattat cttactaatc atttttgcta
    15301 cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga
    15361 ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag
    15421 ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc
    15481 ctaaacgtgc gattcctaaa gccatcaaag gtgtcatggg aagaatcatt tccttttaca
    15541 tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg
    15601 tcagtccttt tgtgattgta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca
    15661 attttgttat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa
    15721 gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa
    15781 acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg
    15841 tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt
    15901 atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa
    15961 aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt
    16021 tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc
    16081 tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa
    16141 atcgatagaa aattttattt tatttatgtg gaatagatgg aactattgaa gaaaggagtt
    16201 gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta
    16261 aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga
    16321 tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg
    16381 ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg
    16441 ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc
    16501 atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt
    16561 atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa
    16621 actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc
    16681 ctgacaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc
    16741 atccaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga
    16801 ccattatccg tccaaataaa aaaagaggat gttccagaag tgatcagctt caagaaataa
    16861 gaagaagcta cttctggaac accgtgaata aggggttgtg aaaaagctgc cctgcatcaa
    16921 gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta
    16981 ccgcagatgc aagcttttga acaccgttta ttcgtaaaag ggattgtgac aaaaaaagtc
    17041 ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc
    17101 ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa
    17161 cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa
    17221 tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc
    17281 accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc
    17341 tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac
    17401 tccgatactt gtcatcaatg ttgtaaaaat caattttggt gtgttcgtca tctttcttga
    17461 tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa
    17521 agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc gactaagcag
    17581 tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa
    17641 cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt
    17701 caacaaaatc gtcaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa
    17761 aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg
    17821 aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga
    17881 aacaacaaat gccggaacag ttgaccacat catatgcttg atgtgtttga acaaatcggc
    17941 atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa
    18001 aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaggccta gtgttgtccc
    18061 aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct
    18121 acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt
    18181 gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga
    18241 acctactaaa ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc
    18301 ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat
    18361 cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc
    18421 taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc
    18481 catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt
    18541 actccgccgc aattcatccg ctattcctcc tttataaaaa taaaaagacc tgttttaaac
    18601 taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca
    18661 aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca
    18721 cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg
    18781 tatgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt
    18841 tctgaaaatt atttttataa ctttcacaca caagtttatt ttttactaga aagtagcacc
    18901 ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt
    18961 tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga
    19021 agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata
    19081 tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga catttcctac
    19141 atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc
    19201 ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac
    19261 tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac
    19321 gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata
    19381 gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat
    19441 cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac
    19501 ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc
    19561 gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata
    19621 acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac
    19681 aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg
    19741 gatttctcca ccagtttcat caataaattg ctccactatg ctggcggcta atacggagta
    19801 agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc
    19861 caaaaaggca attgtgggtc cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg
    19921 gtgtccagct acttcttttt ctacccaaaa cccttctttt tctaattgtt taatcaacgc
    19981 tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag
    20041 tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt
    20101 tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc
    20161 ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt
    20221 ttgataagcg tctactattt tttgatacgt tttatttttt tcatcttctt ttcttgcaac
    20281 gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga
    20341 agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc
    20401 tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt
    20461 ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc
    20521 agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac
    20581 ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg
    20641 tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc
    20701 taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccgcatt
    20761 tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg
    20821 gtctttcacg ttgtcccata catctgtatc ttctccaata attcctagtt ttacggtagt
    20881 ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc
    20941 tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat
    21001 gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa
    21061 tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag
    21121 caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt
    21181 tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac
    21241 gtaaaataat accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat
    21301 caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca
    21361 atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg
    21421 ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa
    21481 gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca
    21541 cacctaagat gattccgatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg
    21601 tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt
    21661 taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact atctaattcg
    21721 tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa tgctttttgc
    21781 cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat
    21841 aactctacat tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg
    21901 attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg
    21961 atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta
    22021 gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgcta
    22081 ctaccttttt caatgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg
    22141 acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt
    22201 tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca
    22261 cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt
    22321 ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt
    22381 agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa
    22441 tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg
    22501 atggcctttc tttcttttcg caattctttg ggagagagct gactcaaatt cgtctgattg
    22561 acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt
    22621 ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct
    22681 acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc
    22741 aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt
    22801 cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca
    22861 caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct
    22921 tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc
    22981 ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca
    23041 aataatattc aaaaaaaaat ccttttacta acataaacta tgtcagtaaa aggacgacgt
    23101 atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag
    23161 agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta
    23221 ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct
    23281 gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa
    23341 tctacatgag gaaaactaaa aagtcaagca cgaaattcaa attatttttt ctatatttat
    23401 ttttctgttt ttttatctaa catttcaata acagttcgaa gatcttttcc ttcagcaaga
    23461 agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga atcaggtatc
    23521 aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga atcttccact
    23581 gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata aggaatcggt
    23641 gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtcaaagta atcagctaat
    23701 ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt
    23761 ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg
    23821 gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact
    23881 ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg
    23941 ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc
    24001 cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca
    24061 cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc
    24121 aaaaataaca gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgatgga
    24181 tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc
    24241 ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa cttgtaaaag
    24301 gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca aatgttacgt
    24361 tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc
    24421 atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact tgctgtgaac
    24481 tatccaatgt atggaaactt ccgtcaacta cagcaggaca aataacaaaa tacatcaaca
    24541 agtaagtagt aatatcaaat atcgaacttg ttgggccaaa gtaaaccatg aatttcccaa
    24601 tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg
    24661 gcaaggaaat gcaggaaaca tcatagatta gattcaaaaa cagaagttgt aaaggcagca
    24721 ttggtaaaaa tggcaggaac atacttgcaa tcagcacgga aaacatattg ccaaaattag
    24781 aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa
    24841 ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg
    24901 caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt
    24961 cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca
    25021 aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg
    25081 ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta
    25141 gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta
    25201 cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat
    25261 caaggaaagt aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc
    25321 caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa
    25381 gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac
    25441 cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca
    25501 tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa
    25561 aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt
    25621 catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat
    25681 acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa
    25741 tgattttatc ttgtgtcaat gtccccgttt tatccgtgca taacacatcg attgcaccaa
    25801 agttttggat ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc
    25861 ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta
    25921 cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga
    25981 taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc
    26041 ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata
    26101 gtgtttcatt tccaacagaa acaatgattc cttccgcact tccacttatg acattacttc
    26161 ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg
    26221 catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct
    26281 ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat
    26341 ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg
    26401 ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa
    26461 tcaacgtcat tgtcccgctg acgatgacca tagtgaagat aatggctaca ccgaaaagat
    26521 ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga
    26581 tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacgatg aatggtgttt
    26641 ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt
    26701 ctgtcaatcc atttgctgat gcgtggaata gttcaaataa ttcttgtttc gtttttccag
    26761 caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt
    26821 gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag
    26881 aaaaaactga aaggggctga gaacagatag ataatagcct tggcctttcc tctagatcct
    26941 tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc
    27001 atgacttcca atcatttttc gaatagttat tttcccaatt agaccacctc ctttttattt
    27061 ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt
    27121 tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa
    27181 cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct
    27241 gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc
    27301 tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt
    27361 caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct
    27421 cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac
    27481 gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt
    27541 tagtcggaag catcattcaa cgttttgaag caaagggact ggccattgct gaaatgaagt
    27601 ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt
    27661 tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg
    27721 gcgaggaagt gatcgatatc gtccgtaaga tggttggggc aacaaaagct gtcgatgctg
    27781 ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt
    27841 ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa
    27901 acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg
    27961 ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata
    28021 tattcgagca ctgtgaagaa ttcttggtag tctttcgtct ttttttcccc taaagcagca
    28081 gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt acttctttct
    28141 ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca
    28201 ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg
    28261 ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac
    28321 cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct
    28381 ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct
    28441 gtctcagata cttctgtttt ttcgaccatg tgaagactca aaaccaaata atacagatct
    28501 tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct
    28561 tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg
    28621 tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat
    28681 gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga agtcttagaa
    28741 cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc
    28801 aattcaccat catacataat agttctcctt tttaatttaa atttttccac attcaataaa
    28861 tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa
    28921 ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat
    28981 tgattatctt tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt
    29041 ttattgccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgatggct
    29101 atgttacgtt ctgcttcatt tttatagtca tattttttcg gatcgatcgt ttcaaatccg
    29161 tttggcacat aaaaacgcaa gagattttct tgattcagct tatctgaaag acttaaagca
    29221 gatttgactt tttcctgatc tttggaaatt tcctcgtcga ttcctgctgc tttcgtatcg
    29281 atgatttctc ctgtttggtt attgtaagct tttcctccta agatggtgta tttgggtgtc
    29341 acgaagttcc catttcgaaa agccactact tgatcatgct gaggagataa taaatccgta
    29401 ccaaaatgga gatagtcttt atcatcgatt cccaagagat gaagtaatgt cggcaaaaca
    29461 tcgatttctc caccgtattc gtgattgact gaccctttag tataaccagg tatatgaatc
    29521 attaatggca cacgctgcat ttgtgcgttg tcaaattcat tccatgtatc tgggtctttt
    29581 ccaagagcac ttgctaagtc tttattatct gtatttgaga tgccaaaatg atccccataa
    29641 atcacaaaga tcgaattttg gtaaatagcg gattttttca ggtacgtaaa aaattgttcc
    29701 aatgactgat ccaaataatg tgccgttcga acatagttat ctactgtgct atttccagtg
    29761 ttcaaagatg gaaaatcgaa attcttatca tccgtataat aaggcgtgtg attggttacg
    29821 gaaaggaatt tcgtgtagaa cggctgttgt agatgttcaa gatattgggc agattcactg
    29881 aataaatctt tatctaatat tccataacct agcgtttcat ctgattcatc aaaataagat
    29941 cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta
    30001 gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt
    30061 gcggcttgaa agacattatc tgacccaagt tctgtgaata atgagccttg aggtaaacca
    30121 aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga
    30181 aaaaaattat caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg
    30241 acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga
    30301 acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt
    30361 ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg
    30421 ctagtcattc ctgttttgat tccatcataa atagtatacg catcgattcc tagatatttg
    30481 acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc
    30541 gctagatttc ctgaaaaaat aagccctgaa agacaggtca cagcgatagc catcggtttg
    30601 ctgacaggtt gacttttgat aggtattttc ttgatcgtca accaaaccag tatcccaatg
    30661 aacgtagcaa tatccagcca gtagaggaca tcatggggtt tcatcaaaga aaaggagctt
    30721 cctgataacc cttgcgacac tttcgaaaat cccaatacag atttgactgt aatgaaatct
    30781 gtgaattccc gataaaaaat aatgttcaag tacaacaaaa gcgtgtttaa aatatcaatg
    30841 atcaaaagaa tggctaatgc tggctttggc tttttaatat acaaagaaag gccaaaaaac
    30901 aataaggtag ttgcaatcgg gttgatccac aagataaagt actggatcgt accttccaca
    30961 cctaacgaaa aatctgcata gtaagcaata atcgtcttca tccagaaaaa gaaaacagct
    31021 gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact
    31081 tctctctatt cacatttttc cttattttgc tcctgaaaaa tgaaaattcc ctaaaaaagt
    31141 tgttatctta agattttttt attctttcta aagattattc cgtgcacttt tcacagcata
    31201 aaaccgctgt gatataagcg attgaaacag taaggtgata aaagtcttgt cgcagctcct
    31261 gtttagttga tgcaggacag ctttttcaca acttcttctt taaacggtgt tctcctgcca
    31321 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    31381 ccttgtggct cagagttaaa cggcaagctc aagacctctt gata
//
LOCUS       DACCQP010000025        30120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCQP010000025 DACCQP010000000
VERSION     DACCQP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.025"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(419..1363)
                     /locus_tag="IVO87_001314"
     CDS             complement(419..1363)
                     /locus_tag="IVO87_001314"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7449520.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1659..2603)
                     /locus_tag="IVO87_001315"
     CDS             complement(1659..2603)
                     /locus_tag="IVO87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449521.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2824..4038
                     /locus_tag="IVO87_001316"
     CDS             2824..4038
                     /locus_tag="IVO87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7449522.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4060..6075
                     /locus_tag="IVO87_001317"
     CDS             4060..6075
                     /locus_tag="IVO87_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7449523.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6158..7531)
                     /locus_tag="IVO87_001318"
     CDS             complement(6158..7531)
                     /locus_tag="IVO87_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7449524.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7557..7841)
                     /locus_tag="IVO87_001319"
     CDS             complement(7557..7841)
                     /locus_tag="IVO87_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7449525.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7843..9858)
                     /locus_tag="IVO87_001320"
     CDS             complement(7843..9858)
                     /locus_tag="IVO87_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7449526.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10037..10342)
                     /locus_tag="IVO87_001321"
     CDS             complement(10037..10342)
                     /locus_tag="IVO87_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7449527.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10431..11462)
                     /locus_tag="IVO87_001322"
     CDS             complement(10431..11462)
                     /locus_tag="IVO87_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7449528.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11669..12544
                     /locus_tag="IVO87_001323"
     CDS             11669..12544
                     /locus_tag="IVO87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7449529.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12696..13538)
                     /locus_tag="IVO87_001324"
     CDS             complement(12696..13538)
                     /locus_tag="IVO87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7449530.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13630..15507)
                     /locus_tag="IVO87_001325"
     CDS             complement(13630..15507)
                     /locus_tag="IVO87_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7449531.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15504..16925)
                     /locus_tag="IVO87_001326"
     CDS             complement(15504..16925)
                     /locus_tag="IVO87_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7449532.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16928..20317)
                     /locus_tag="IVO87_001327"
     CDS             complement(16928..20317)
                     /locus_tag="IVO87_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7449533.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20574..21965
                     /locus_tag="IVO87_001328"
     CDS             20574..21965
                     /locus_tag="IVO87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7449534.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22038..22685)
                     /locus_tag="IVO87_001329"
     CDS             complement(22038..22685)
                     /locus_tag="IVO87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7449535.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22693..23378)
                     /locus_tag="IVO87_001330"
                     /pseudo
     CDS             complement(22693..23378)
                     /locus_tag="IVO87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(23384..24457)
                     /locus_tag="IVO87_001331"
     CDS             complement(23384..24457)
                     /locus_tag="IVO87_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7449536.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24679..25353
                     /locus_tag="IVO87_001332"
     CDS             24679..25353
                     /locus_tag="IVO87_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449537.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25358..26698
                     /locus_tag="IVO87_001333"
     CDS             25358..26698
                     /locus_tag="IVO87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7449538.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26847..27833)
                     /locus_tag="IVO87_001334"
     CDS             complement(26847..27833)
                     /locus_tag="IVO87_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7449539.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28557..29588
                     /locus_tag="IVO87_001335"
     CDS             28557..29588
                     /locus_tag="IVO87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7449540.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29697..>30120
                     /locus_tag="IVO87_001336"
     CDS             29697..>30120
                     /locus_tag="IVO87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7449541.1"
                     /translation="MMETITYSLLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         8931 a         5638 c         5406 g        10145 t
ORIGIN      
        1 tgataaacct ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc
       61 ttatgcaaaa ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa
      121 tgataaaaac cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat
      181 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
      241 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      301 cgattaattt taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct
      361 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      421 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      481 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      541 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      601 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      661 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      721 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      781 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      841 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      901 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      961 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
     1021 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
     1081 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
     1141 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1201 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1261 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1321 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1381 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1441 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1501 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1561 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1621 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1681 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1741 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1801 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1861 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1921 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1981 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     2041 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     2101 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     2161 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2221 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2281 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2341 tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac
     2401 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2461 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2521 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2581 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2641 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2701 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2761 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2821 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2881 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2941 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     3001 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     3061 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     3121 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3181 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3241 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3301 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3361 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3421 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3481 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3541 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3601 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3661 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3721 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3781 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3841 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3901 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3961 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     4021 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     4081 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     4141 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4201 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4261 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4321 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4381 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4441 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4501 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4561 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4621 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4681 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4741 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4801 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4861 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4921 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4981 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     5041 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     5101 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     5161 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5221 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5281 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5341 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5401 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5461 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5521 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5581 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5641 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5701 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5761 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5821 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5881 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5941 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     6001 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     6061 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     6121 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6181 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6241 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
     6301 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6361 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6421 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6481 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6541 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6601 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6661 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6721 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6781 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6841 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6901 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6961 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     7021 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     7081 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     7141 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7201 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7261 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7321 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7381 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7441 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7501 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7561 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7621 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7681 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7741 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7801 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7861 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7921 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7981 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     8041 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     8101 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     8161 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8221 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8281 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8341 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8401 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8461 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8521 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8581 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8641 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8701 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8761 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8821 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8881 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8941 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     9001 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     9061 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     9121 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9181 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9241 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9301 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9361 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9421 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9481 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9541 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9601 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9661 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9721 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9781 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9841 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9901 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9961 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
    10021 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
    10081 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
    10141 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10201 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10261 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10321 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10381 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10441 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10501 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10561 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10621 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10681 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10741 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10801 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10861 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10921 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10981 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    11041 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    11101 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    11161 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11221 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11281 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11341 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11401 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11461 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11521 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11581 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11641 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11701 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11761 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11821 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11881 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11941 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    12001 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    12061 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    12121 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12181 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12241 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12301 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12361 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12421 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12481 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12541 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12601 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12661 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12721 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12781 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12841 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12901 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12961 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    13021 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    13081 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    13141 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13201 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13261 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13321 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13381 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13441 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13501 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13561 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13621 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13681 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13741 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13801 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13861 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13921 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13981 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    14041 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    14101 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    14161 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14221 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14281 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14341 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14401 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14461 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14521 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14581 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14641 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14701 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14761 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14821 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14881 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14941 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    15001 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    15061 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    15121 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15181 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15241 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15301 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15361 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15421 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15481 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15541 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15601 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15661 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15721 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15781 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15841 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15901 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15961 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    16021 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    16081 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    16141 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16201 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16261 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16321 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16381 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16441 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16501 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16561 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16621 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16681 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16741 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16801 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16861 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16921 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16981 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    17041 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    17101 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    17161 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17221 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17281 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17341 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17401 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17461 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17521 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17581 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17641 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17701 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17761 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17821 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17881 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17941 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    18001 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    18061 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    18121 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18181 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18241 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18301 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18361 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18421 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18481 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18541 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18601 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18661 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18721 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18781 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18841 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18901 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18961 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    19021 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    19081 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    19141 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19201 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19261 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19321 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19381 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19441 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19501 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19561 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19621 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19681 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19741 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19801 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19861 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19921 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19981 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    20041 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    20101 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    20161 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20221 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20281 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20341 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20401 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20461 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20521 gggttgaggg agaaaaacat atgatcaggt tgttggaacg aaactataga aagatggaaa
    20581 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20641 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20701 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20761 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20821 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20881 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20941 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    21001 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    21061 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    21121 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21181 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21241 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21301 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21361 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21421 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21481 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21541 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21601 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21661 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21721 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21781 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21841 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21901 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21961 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    22021 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    22081 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    22141 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22201 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22261 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22321 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22381 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22441 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22501 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22561 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22621 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22681 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22741 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22801 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22861 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22921 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22981 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    23041 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    23101 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttc tggaagctca
    23161 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
    23221 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
    23281 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
    23341 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
    23401 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
    23461 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
    23521 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
    23581 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
    23641 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
    23701 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
    23761 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
    23821 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
    23881 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
    23941 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
    24001 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
    24061 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
    24121 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
    24181 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
    24241 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
    24301 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
    24361 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
    24421 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
    24481 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
    24541 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
    24601 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
    24661 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
    24721 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
    24781 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
    24841 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
    24901 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
    24961 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
    25021 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
    25081 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
    25141 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
    25201 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
    25261 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
    25321 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
    25381 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
    25441 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
    25501 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
    25561 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
    25621 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
    25681 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
    25741 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
    25801 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
    25861 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
    25921 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
    25981 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
    26041 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
    26101 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
    26161 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
    26221 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
    26281 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
    26341 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
    26401 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
    26461 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
    26521 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
    26581 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
    26641 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
    26701 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
    26761 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
    26821 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
    26881 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
    26941 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
    27001 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
    27061 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
    27121 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
    27181 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
    27241 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
    27301 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
    27361 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
    27421 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
    27481 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
    27541 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
    27601 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
    27661 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
    27721 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
    27781 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
    27841 cctcctagca aatagattta tgattggtat attgttactt ataaatttat tctataggag
    27901 acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta attgaaattt
    27961 gaaaacattt atgttaatga aagtcatatt ctaataaaat gtcatttgtt tctatatgaa
    28021 tttttatgaa aaatttcata taatttccac tcgttgtgat ggtttaattt atccaatcca
    28081 tcaataagca aacaacttat ataaaaaagg attaattctc aatataattg ctcctaccat
    28141 agaataaatc ttcatgtaaa gtaatgaagt accaactatt tctaatactt ctttaagaat
    28201 tatcccaaca atatcgttag acaaaccatt attgaataaa tggagagcca atcgattctg
    28261 aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc ctcctgccat tagaacgaac
    28321 caaatccatt tcattgctca tgaagtaacc acatagctaa aaagaggata aatcaattaa
    28381 aagatcaaaa gaatgactgc actaataaaa taactaaaaa tgtatcttct tacttcatat
    28441 cttttagcta gtacttagtt aaaaatttgc ttcctaagat caagacaaac tgtctatctt
    28501 catttgatga aatgcggtaa aatatagaaa atgaacacta ttggagggaa tttgtaatgg
    28561 acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca acgtacacac ttgctccctt
    28621 ccgtctttca acagttttta gaagaaaatc gttcttccct ttcattaggc agtcgctatg
    28681 aatacgcaaa agattttcat ctgtttactg attatttgag aaaagtattt tctgaagaag
    28741 tggatgatca gctgattatc catttagaaa aacaagatta tcagaatttc ttagctaaaa
    28801 tctatcgaca tactcgttcg ttccaaacta cagcgaacca agaaacggag caattgtttg
    28861 aaaatagcta ttacgggatt tcaaggaagc aatattcatt aaatcatttt ttacgatttt
    28921 tgtatcaaaa aggactgatt gaagaagaga tttctctttt gggtacaagt ttaccggaaa
    28981 caaccaaagc agctccgcga tatatcgatg cagaactttt caaagatttt gagcatttgt
    29041 ttatcccgat tgaaggcttt caaacaagca gagaagctag ctttgaagaa aagaatagac
    29101 cgaggaacct tgcaattact gccattcttc tgtattgcgg attaaagatc catgaagtag
    29161 tagaacttaa acgtaaagat gtatctttga ccaagcagat tcttaatctg aatagaaaag
    29221 aaaacagact gaataaagta ccaataccaa aagaagcgat gccatttctg gagaactatt
    29281 ttaagttaac agcacaaaat acggacgaaa attcttttgt ttttcgttcc tcacatgatc
    29341 agcaaatttc tcctcggact attcgccaaa ttttgagcaa aattacagta tataaaaacg
    29401 tatcgccaga actatgcaga aaaacatatc gttattatgc agaactatat ttagatgatg
    29461 ctgacgcggt aaattattta tgtggtaatc gatacctctc atcccattct tttcaagata
    29521 tctgggaaaa gatggttgat ttttcttatc acgaattagc ggcattcgtt cgggtttctt
    29581 ccatataaaa caaatgtatg atcttttttt cacaataaac tatgttatta tataaaggat
    29641 tatgttacga ttatcttgaa tttgtatatt acttaacaaa agataatgga gtgtgaatga
    29701 tggagaccat aacgtattcc cttttgaact tatttacgaa ttttcaagaa gctgcagcta
    29761 acttttccga gaccccgatt atctttgatg agccactttc agctttccca gaattaggac
    29821 ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat caattggctc
    29881 atcttggagt gaaatccgga gacaaagtta ttatttataa gagttccgct tttgacacat
    29941 accttctagc tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc
    30001 taccaacaac aacaatggaa gtatttatcg atcgtttaga agaccctttc attttatttg
    30061 atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca aaacagattc
//
LOCUS       DACCQP010000026        29423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCQP010000026 DACCQP010000000
VERSION     DACCQP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.026"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(266..466)
                     /locus_tag="IVO87_001337"
     CDS             complement(266..466)
                     /locus_tag="IVO87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7449542.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IVO87_001338"
     CDS             complement(771..2003)
                     /locus_tag="IVO87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7449543.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="IVO87_001339"
     CDS             2257..2826
                     /locus_tag="IVO87_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7449544.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="IVO87_001340"
     CDS             complement(3004..3444)
                     /locus_tag="IVO87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7449545.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="IVO87_001341"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="IVO87_001341"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7449546.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..5321
                     /locus_tag="IVO87_001342"
     CDS             4398..5321
                     /locus_tag="IVO87_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7449547.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5397..6536
                     /locus_tag="IVO87_001343"
     CDS             5397..6536
                     /locus_tag="IVO87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7449548.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6529..7329
                     /locus_tag="IVO87_001344"
     CDS             6529..7329
                     /locus_tag="IVO87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7449549.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7329..8156
                     /locus_tag="IVO87_001345"
     CDS             7329..8156
                     /locus_tag="IVO87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7449550.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8134..8868
                     /locus_tag="IVO87_001346"
     CDS             8134..8868
                     /locus_tag="IVO87_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7449551.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8968..9834
                     /gene="rfbA"
                     /locus_tag="IVO87_001347"
     CDS             8968..9834
                     /gene="rfbA"
                     /locus_tag="IVO87_001347"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7449552.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9848..10420
                     /gene="rfbC"
                     /locus_tag="IVO87_001348"
     CDS             9848..10420
                     /gene="rfbC"
                     /locus_tag="IVO87_001348"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7449553.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10442..11470
                     /gene="rfbB"
                     /locus_tag="IVO87_001349"
     CDS             10442..11470
                     /gene="rfbB"
                     /locus_tag="IVO87_001349"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7449554.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11568..12419
                     /gene="rfbD"
                     /locus_tag="IVO87_001350"
     CDS             11568..12419
                     /gene="rfbD"
                     /locus_tag="IVO87_001350"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7449555.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12453..14486
                     /locus_tag="IVO87_001351"
     CDS             12453..14486
                     /locus_tag="IVO87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449556.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14530..15810
                     /locus_tag="IVO87_001352"
     CDS             14530..15810
                     /locus_tag="IVO87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7449557.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16020..16826
                     /locus_tag="IVO87_001353"
     CDS             16020..16826
                     /locus_tag="IVO87_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449558.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16838..18058
                     /locus_tag="IVO87_001354"
     CDS             16838..18058
                     /locus_tag="IVO87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449559.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18048..19634
                     /locus_tag="IVO87_001355"
     CDS             18048..19634
                     /locus_tag="IVO87_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7449560.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19673..21811
                     /locus_tag="IVO87_001356"
     CDS             19673..21811
                     /locus_tag="IVO87_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7449561.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22179..23162)
                     /locus_tag="IVO87_001357"
     CDS             complement(22179..23162)
                     /locus_tag="IVO87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7449562.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTYH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23540..24931
                     /locus_tag="IVO87_001358"
     CDS             23540..24931
                     /locus_tag="IVO87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7449563.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQDFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24947..25987
                     /locus_tag="IVO87_001359"
     CDS             24947..25987
                     /locus_tag="IVO87_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7449564.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26006..27091
                     /locus_tag="IVO87_001360"
     CDS             26006..27091
                     /locus_tag="IVO87_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7449565.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27124..28086
                     /locus_tag="IVO87_001361"
     CDS             27124..28086
                     /locus_tag="IVO87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7449566.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            28079..>29423
                     /locus_tag="IVO87_001362"
     CDS             28079..>29423
                     /locus_tag="IVO87_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7449567.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVL"
BASE COUNT         9519 a         4785 c         5866 g         9253 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     5281 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
     5341 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
     5401 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
     5461 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
     5521 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
     5581 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
     5641 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
     5701 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
     5761 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
     5821 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
     5881 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
     5941 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
     6001 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
     6061 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
     6121 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
     6181 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
     6241 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
     6301 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
     6361 tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
     6421 tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc
     6481 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
     6541 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
     6601 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
     6661 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
     6721 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
     6781 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
     6841 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
     6901 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
     6961 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
     7021 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
     7081 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
     7141 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
     7201 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
     7261 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
     7321 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
     7381 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
     7441 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
     7501 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
     7561 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
     7621 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
     7681 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
     7741 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
     7801 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
     7861 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
     7921 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
     7981 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
     8041 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
     8101 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
     8161 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
     8221 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
     8281 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
     8341 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
     8401 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
     8461 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
     8521 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
     8581 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
     8641 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
     8701 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
     8761 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
     8821 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
     8881 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
     8941 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
     9001 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
     9061 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
     9121 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
     9181 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
     9241 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
     9301 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
     9361 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
     9421 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
     9481 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
     9541 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
     9601 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
     9661 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
     9721 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
     9781 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
     9841 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
     9901 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
     9961 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
    10021 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
    10081 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
    10141 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
    10201 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
    10261 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
    10321 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
    10381 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
    10441 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
    10501 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
    10561 aaacaaagag aacttggctg gacttcctag cgatcgcgta gaattggtcg taggagatgt
    10621 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
    10681 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
    10741 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
    10801 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
    10861 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
    10921 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
    10981 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
    11041 tcgccaagta acgaatatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
    11101 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
    11161 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
    11221 gatggaattg atcttagaaa tgatgggtca accaaaagat gcgtacgacc atgtaaacga
    11281 ccgtgcagga cacgacttgc gttatgcaat cgattcgaca aaattacggg aagagttagg
    11341 atggaagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
    11401 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
    11461 tggacaataa tacagagaaa atgtcactgt attaatggaa aaacacatgt gaggcaggcg
    11521 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taatcatatg tttttattaa
    11581 ctggaggaaa tgggcaacta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
    11641 aatatgtttc aacagatgca aaagacttgg atatcacaga tgccgagaaa acaatggctt
    11701 acatcacaga attaaaacct gatgttattt tccattgtgc agcttacaca gcagtagata
    11761 aagcagaaga agaagcaaaa gaattagatg agaagatcaa tgttgatggt acaagaaatg
    11821 tagcacttgc agcaaaagct gtgaatgctc aattcgtcta tatcagtaca gactatgttt
    11881 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
    11941 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
    12001 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
    12061 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
    12121 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
    12181 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgaattcgca aaagaaatat
    12241 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    12301 cacgtccgca gtattcagtc atgagcctga aaaagacaga agagttagga tttgtcatac
    12361 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    12421 ttccttttat caaatagaat ttggagcatg atatggaaaa acaaacaaga agtgcacgac
    12481 gaaaagaaaa gccaaaagcg aaccaggcag attcgaattt ggtgcttgac ctgtttctaa
    12541 aactccgcta tcttattggc gttttagtca tcgtggtcat cgttactttt aatttaaacg
    12601 gaagttcact tggtgtttgg gataaatatg tgtcgcaaag agacgatggg aaaaaaactg
    12661 atgtgatttt tggacagaat cgggaagtgc ggtcagacga atggctggtg cagacacctt
    12721 tttacctctc acaagctgaa gaggactttc cgttggtgaa cgaggattat tcattaaacg
    12781 gacaaaacat gatcattgcg tataattctc ctgtaaagga tattacggtt atcggaaaac
    12841 cattcaactg gggattcttt ttcctcggaa gagatcgagg actttccttt tattgggcga
    12901 tgaaattagt cgtcatggtc ttgcttggat tcgaagtact gatgatatta acgaagagaa
    12961 aaaaagctct atcgctaatc ggtgcatttg cgctgacatt ttctccagca gtccagtggt
    13021 ggttcatgca acatgtgggc gatttgattt tctttacttt gggactgatg actgcttttt
    13081 atcattattt ctacaatcat gataaaaaat ggctacgaac gttgatgatg cttttggtgg
    13141 ttattttcgg tattgggttt attttagtga tttacccagc ccatcaagtg atgctggcgt
    13201 acttgctgat tttttatttt gttggcctgc ttatttacta tagcaagaaa gtttcatggg
    13261 atctgttgga tgtagtgttg attggagggg cagttttatt tattggcgga gtcatggttc
    13321 atttctggct aacgtcaaaa gacgcattgt tagcttcttt gaatacctta tatccaggaa
    13381 aacgggtaag tactggagga aagtggacga ttggcgaatt tttttctttc cttacaaatt
    13441 ggaaaattcc ttttgaagat atcaactatt cgaataactc tgaagtggcc ctgttctatc
    13501 acttttttcc aactgtattc ctagcaagtc catttgtgtt atttggcaaa aaagatagcg
    13561 aacaaaagtt actgggacgt attttgatga ttttttgtct gttttctatc ttttggatta
    13621 ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct gtcttatgtt cctccattaa
    13681 gagccatcct tacttttagt tttgcggctt gtttattatc gatctggttt atcgcttata
    13741 tttggagaga agatgttatt cctatttggt ataaattcat tttgttggca gtaaatgcat
    13801 ggtcttattt ctatgcgatt acgcattcga aaatggataa ttatttttcg gattttgaaa
    13861 ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt tgcattattt ggctggagac
    13921 gcttgtttta cctcgctttt gccgccttag ttatcgtatc tggctttttc gtcaatccag
    13981 tcgtagaagg tacaggagcg atttttgaaa aaacactggc aaaagaaatc caaaaaatcg
    14041 atcaacaaga tcctggtaaa gtttggttgt ctgaagatga attatataac ttcaccccaa
    14101 cattgggagt gaaatccttc aatacagtac gcttttatcc agatatagct gcatggaaga
    14161 aaatcgatcc gaaagaagaa tacgagaagt attataatcg gtatgctcat acaagagctt
    14221 ttatcgctaa ggaagataca aagttcattt tagatcgtcc agatatgttt tcggtcacgt
    14281 tgaattttga agacgcagaa aaattaggga tcgactatgt gatcagtaaa cgaccattaa
    14341 ctgattacaa ccaattatat gaagcgcagt tcacgcagct atatggaccg gataaagacg
    14401 gttatatgat ttttaaagta gaatatccgg attatccaga tgtaccacaa acacaaactc
    14461 aggaaaatca gctagtcgta cagtaatagg ctagctgaga ataatcctga aatgttagta
    14521 gaaaggagag tggagataat tcaagcaatc gtacaaaaat ttgtcaacaa aatgagtgtc
    14581 ggtttcttgc ttttgttact gacgctagtc ttttttgttt ggacagcagg gttaggtttt
    14641 ccagcaactt tgtttatata taacaatagt tcattactta tcgcttttag tgtggctatt
    14701 ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt ttctttatct aggtgttgcg
    14761 cttgcccttt tcattttctt ttcttacttt cgggaattaa gaaatgagtc tttatttgga
    14821 gacttactgt ttcctttgat tattttattt tgtttagtaa ttaaatggat cgagtggaca
    14881 aaatgggatc gcggtattta tctgattgtc ttttcggtct tcctcggaat cacgttatat
    14941 cgtgtattta cagagatcca agtaccagaa ggacaaagca tctgggctcc aggcaattcg
    15001 ctttctgata tttggatcaa tacgaatata atcggaagtt ctttgatgac tcttgcttta
    15061 ttgatcagtg gatttgccag ttcttttgaa cgatggtatg tacgaatcat cggtgcagta
    15121 gcagttgttg cagcttttct cgggatttgg gtctgccaat ctagaggtgc attagtcgcg
    15181 ttgattgttt ttgcgatatt ggatctttta cctaaatcgt tgatgcgtgt cattcgtgcg
    15241 ccatttattg cttacacggt tacgattctt ttagctttgc cgatttctta tctggcggct
    15301 gtctctgaaa aagtcaattt gtttaccggt cgagaagata tttggcataa attctatcag
    15361 acgctaggag aaaaatctga acaaatttta ctaggaatga aaacatttat cttccaacga
    15421 ggaaaccaat ttttaggaaa ccacaatagt tataattcta ttttgaatat ctatggattg
    15481 ataggatttg ggattgctgc tttgctgctt atccttttta ttggtcgctt aacgttgaaa
    15541 gcagatttat ccaacgggca gatgactttt atctgggcct tttttgcggt gatgatgcag
    15601 tcatttatgg aggatacatt gacttcatac ccatgggttc cgattattta tatcctgttg
    15661 gctatggccg ttcatcgata tgatgaacca gaagttgcgg aggaattcta tgttatggaa
    15721 gaggcatatg acgatatgac ttatgatgat acatcttatg aggaacaacc agatagcgct
    15781 cattcttcca ggatccaacg ctatcattga taggcacaaa atacgagaga ggctgtgaca
    15841 accgtcgcag ccttttttgg tttattgcaa ttgtctagca tcttctgtaa aatagaaacg
    15901 ttagagaaaa tcttgtatca tagaaaatta agaaagaaat aagaacgcag ctcggtacca
    15961 aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa atcgcttgga ggaacacaaa
    16021 tgtttaaaga tttgtattta tttatgaagg acatttatca gaacagaaaa ttattgctac
    16081 agttttcgat caatgacttc aaatcaagat atgcaggatc gtttttagga atcttatggg
    16141 cgtttatcaa tccgctattt actgtattga tttattggct ggtatttggt gttggactga
    16201 aagcacaaat gatcgatgca aaatacccat ttatcgtata tttagttacc gggatgattc
    16261 cctggttctt cttctcagat gcatttgcat caactacggt tgtttttcgg gaatatactt
    16321 atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc cacgacgaaa atcctatcca
    16381 atctgtacac ccatttattc ttcattctga ttggcatagc tgtcgcgctt ataaatggta
    16441 tatatccttc agcaatgtct ttacagctga tttattattt attttgcatg tgtgctttct
    16501 tgacaggatt gacttggctg acggcatcga tccaaccatt tttacctgat atcatgcaat
    16561 taattacgat cgtgctgcag ttgatcatgt ggacattacc gatattatgg agccctaatc
    16621 agtttgattc aggtcttgta agcatattga aattaaatcc gctgtactat gttgttcaag
    16681 gttatcgtga gtcattttta agtgaagctt ggttctggga gcatggaaca tatactttgt
    16741 atttctgggt tttcacgtta gtaatgttcg taatcggctc tgcagtgttc cgtagattga
    16801 aaccacattt ttcagatgtg ctgtaaggag aatagctatg acagaatatg caattagact
    16861 aaaagatatc acaaaatctt ataatatgta cgcaaaacct tcggatcgat ttcgtgaagc
    16921 cctcaatcct tttaagaaat cctatcatga tgtattttat gcgttgaaaa acgtgaatgt
    16981 agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa aacggatcgg gaaaatctac
    17041 aatgttgaaa atcattacgg gagtactgac accaacatcc ggcacgatgg aaatcgaagg
    17101 aaaaatctca gctttgctag aactaggatc aggattcaat cctgagtatt ctggctatga
    17161 aaatatctat ttaaatggaa tggttctagg attttcaaga gaagaagtcg atgaaatggt
    17221 cgatgatatc attgaatttg ctgacatcgg agaccatatt tatcaacctg taaaaactta
    17281 ttcaagcggg atgttcgttc gtctagcttt tgctgtagca atcaatgtga atcccgatat
    17341 tttgattgtg gatgaagcat tggcagtagg cgatcttgaa tttcaactaa aatgtatgga
    17401 aaaatttaca gaaatcaaga actcgggaaa gacgatttta ttcgtttctc atgatatcag
    17461 ttcgattcgt cgtttttgtg atcgttccta ttggttgaaa aacggagaag tcgtcgaata
    17521 tggcgatacg atggaagtaa cgacgaatta tgaaaacttt ttaaagaaaa aatcagttaa
    17581 aacagtggat cggaataaaa accaagtaga acggttcgcc gctccagata tcgtcgacgt
    17641 gacgaaagcg gaattgttgg ataaagacaa aaatccggtg gatatgctgg aacaaggaga
    17701 aaaactatac gttaaagtaa cttatgaagt aaaagatgat acaatcaaaa atccggtttt
    17761 aggaattgcg ctacgaacag tcgacaattt ttatgtatgc ggacttaata ctctactaga
    17821 tggtatcaag attccttgga aaaaaggaga gaatatcttt tatctggagt atgaaaagtt
    17881 aggtttgtta gctggtgaat attattttga cgttgcggtc tttgaagaaa atgcaacggt
    17941 tcctttggta tataagacaa agtacatgaa cttgtttgtc agcggttctt atatcggaga
    18001 aggcatcgtg gtcttagacc ataaatggga agaagggact catagcaatg aaatatgatt
    18061 ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat tgccgcacag atcaaaccag
    18121 gaagtaaagt tttagagttt ggtcccggaa acggccgatt aacgaaacat ttaatcggag
    18181 caaaacaatg tgaagtgagc attgttgagt tagataagga attgttcgat tttgtcagcg
    18241 aatttgctca agatggattt tatggtgata tcgaaagttt tgagtgggca aactattatg
    18301 ctggacagac atttgattac gtcttgtttg cggatgtttt agaacatttg gtagatcctg
    18361 gaaaaacatt gaagaaagtc agagaatttt tgaatgaaga aggagagatc ctgattacat
    18421 tccctaactt ggcgcataat tctgtcatga tcgatttgtt caataaccaa ttgccgtggg
    18481 cttcttatgg tctcctagat gaaacccata attcttttta tactcatgat ggcttccaaa
    18541 aagtttttga aaaagcggga ttattcatta atattgaaga ttatttgtac ttagctgtag
    18601 gtgatacaga attaaaatca acttatgaag aattgccgga agctgtacgc tatgatttca
    18661 aaatgcgacc atttggtgaa gtctaccaat actttttctc attgatgaaa catccagtag
    18721 cacaaagttc cattgcagag ccacaaaatt ctaattatgt aaaagtgtta gaagtaaccc
    18781 agcaaacgaa acaagatgaa acaatccaac aaataccatt caataatttc acaggggaaa
    18841 atgagacact aagtttcccc gtatcagaaa caacagaaag aatggtgttt cgcttcgcgc
    18901 aacaacctag ctttatcgaa ttcagcgcag aagctgccgg agaaaaacta acatttatcg
    18961 attcgaatgc tgtagtcaaa acagtaaatg attgttattt gtttgatggg aaagaattac
    19021 cagagttcgt tctaacggat atttctggga aagaagtgac tatccattgt cactatcgct
    19081 ttatcgggga attgacacca acaatgaaag aattgttgga aacgattcgt cccatggctg
    19141 aagtcacaaa gcagctttct gagaaaaatg acgaattaga aagagaaaat catcatcttc
    19201 ttgaggaaaa tacaagacta gaccatacgt taaaaacaac cacaaatcgc tattgcacat
    19261 tgttggaaag tgatgaatgg acaataaaac atagactagg acgacgtaag aaagaaactt
    19321 cgaaaaaaat ccaagaaaaa gaactttcta tatgtattga cgagaaaatc tgggatgcag
    19381 aaacaaaaat attaaaaatc atcggatggg gaatcgctaa ttcggatcgc cagcccttgt
    19441 cctataagtt atcagctgac caatcgccat ttttcgaagc aattcctgtt tcacgagaag
    19501 aagtcaatca agcagaacag ctcgcaaaag gaacagaagc gggatttgag ttacgaatcc
    19561 tttgcgagca agaacgttca ttcttagtgg aagcagtagc tcaaaatggc caatcatgga
    19621 tcattgaaat gtaaataatg attaacttat aaagataagg agtaaacgaa tcgtgcaaaa
    19681 aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa cgggccactg gcgatttgct
    19741 tattgtgggc tgggcaattg atgaagtgac caaagaaatc cctacgataa aggtagaaaa
    19801 agaaaatgtg attgctgaag caacacatgt cgtaagattg gatatcaatc atttatataa
    19861 tcttgatgtt aagacccaaa gcggttttaa ggtacgttta tcagggaaaa tgagaggaaa
    19921 agccatctta gacttccaaa cagcgagaca tcaaaatggc atagctgtga aattaaacgg
    19981 taaataccct tatgatgacg gcattgaatc ttcatgggaa agaaaaaaaa gattactgaa
    20041 aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa gcgatccgtc gggtcaaatt
    20101 ggaattgaag ccaggatcga tcgactatgc agagtggatc agtcgtcatg agaaaccaga
    20161 tttgaaagag caaaggaaag aagcacaagg atttgcgtac cgtccattga tttccatact
    20221 tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa tgcattgatt cggtactgga
    20281 tcaaacgtat gatcattggg aactatgtat cagtgatgat gcttctactg atccagctat
    20341 ccgaaagtgt ctggaatcct atcaagcaaa agatgataga atcaaagtcg ttttccgaaa
    20401 agaaaatgga cacatcagtc tggcaactaa ttctgcattg gaaatagcag aaggagaatt
    20461 tatagcactc ttagataatg atgatgaatt accgccattt gcattatatg aagtggcaaa
    20521 agtgctaaac gtacatccag aactggatgt gatctacagt gacgaagata aaatcgatgc
    20581 agatggcaat cgctttgatc cgcatttcaa agcagactgg tcgcctgata cattgatggg
    20641 aaataattat atctctcact taggcgtata tcgaacaagc attgttaaag aacttggcgg
    20701 tttccgtaaa ggctatgaag gttctcaaga ttatgatttg gtccttcgtg tgacagaaca
    20761 aatccctgca gatcatattt accacattga ccgagtcttg tatcattggc ggacgatccc
    20821 aggatcaact gcaagtaatg gcgaagccaa aagttatatc tatgattccg gagtgaaagc
    20881 attgacagat gccctgtcta gaagaaacat caaaggaagc gtacgtcctg gcagaatttc
    20941 tggattttat gagattgctt atgacgtctt gcaagaggat ttggtcagtg tgatcattcc
    21001 aacgaagaat ggctatgaag atctaaaaac ttgtgtggat tcgatcatcg aaaaaacgtc
    21061 ttattcaaat tatgaaatca ttattgcgga taacggaagt acagatccaa aaatgcaaga
    21121 attatttgca gaatacaagc atcagctgaa agaccgcttt atcgtagaat tgattgacat
    21181 tccattcaat tattctcgaa ttaacaattt agcagcagaa aaagcaaacg gcaaatactt
    21241 tttattcttg aataacgata cagaagtgat tgagccagat tggatgacgg tgatggtttc
    21301 ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa ctgttttatc cagatgatac
    21361 aacgcagcat gcaggagtat tattaggaat cggtggtgtt gcagggcatg cattgaataa
    21421 ctatgatcga acacattgtg gttatttcgg ccgattggtc attgacgtca attacttagc
    21481 agtcactgca gcttgtatga tggtcaaggc agctgacttc aatgctgtta acgggtttga
    21541 tgaaacatta gaagtagcat tcaacgatgt tgacttgtgc ttgaaagttt atgaactggg
    21601 tcgttacaat gtctatgcac atcaagcaga attgtaccat ttcgaatcta aatcaagagg
    21661 ttatgaagat actccagaaa aacaaagacg ttttgctggt gaaatcaaaa aaatgcaaga
    21721 taaatggcca gcatatattg cgcatgatcc attttacaat gataatttaa caaaagaagg
    21781 aatcggcgac ttttcgttga gaccagatta aactataata aaaagctgtt atgttgtgat
    21841 tcgctcgcac ataacggctt tttatagttt tttgtcaaaa aaaggcagaa ggtgccgact
    21901 caaaaaaata agtcgaggtt gggacagaag tgtttaactc cgagaaataa gaagaaattc
    21961 acgaaaattg cttttcaaat ttttgtgaat ttcggcttat ttaagaagaa gttgcttctg
    22021 ctcccaccgt ttattcgttt ttaaagaatg agacaaaagt gtttcttact tttgtcccac
    22081 gctcttattc atgctcagag cgccaataga tacaaaatca tagccaaagt ttcacgaacc
    22141 ctagctgtca aagaatcgat attttatttt aatataggct attttaaaat aaatggttta
    22201 aggtattcct ctacaaaagc cactctatca attatcatgg gtttaaaata agaagtgatg
    22261 gagataacca gattgttctt agcgtcaata taaagaacat ttccactatt accaattgct
    22321 gaatatattt cgttctcaga atctaatacc caccataaat agccatatct catattttgt
    22381 aagtcttcta aagcactggt tctaggtttg aacatctcgt ttatccacat gggtggtacg
    22441 atttgttttc ctttatacat ccctttattt aggcataata caccaatttt tgccagatca
    22501 cttgccgata aacacaaacc atatcctgcc gtcccgatat ttagtggatc acaaaaccag
    22561 acattggttt taggagactt atccattgta aactgcttat gttcttcagc tgtttcagca
    22621 taataattga catggtttgg tatccctata ggatcaaata gatacttatt ggcaaaatca
    22681 acagttgtta gcttacttgt ctgatacaac aagccagtta atatgtgtaa acagacagtt
    22741 tgatagttga attcgccggt tatacctttt cttcctccca ataaatctag tgatgttttt
    22801 gtccaatctt cacttgaaca gacttttgtc catggatcgc ctttacattt gtagggtgct
    22861 ttcattgtta gcaagtgctt gattgtaata gcgtaaatag ttttctcccc gcgtttcact
    22921 ttatactctg gaaaataatc taatacttta tcatcgatac ttttgattaa tccttgctct
    22981 aaagcaatgc caatcagcaa agatacaata cttttcgttg ctgacatgat atgataagta
    23041 tcgtcttttt tataaccatt ccattcatct gtataaactt ctaccccatc cttatatgcg
    23101 acaatttgac atatatttgg ttgtcgaaaa ccaataaaat catgcaactc ttgtttattc
    23161 ataagatatt acctcacttt cttctataag cataatgtca ttaaaaatcg atttcaattt
    23221 tttcgattct tcacgtaata caggtattct tatggactag acagcgcttt gggaagatgg
    23281 attctctgaa aaatataaag gttcacttta tgagtggata aaaaaagcag caaatacttc
    23341 gacttcttta atttaccaac attagtgaaa atcgattcat ctggagattt gtaatttaga
    23401 gtccttgtct tgattagagg tgtataaagc tttttattag acatttttaa attccattac
    23461 aatttgctta taaactaggt ttttaaaggt gactatggta agataataga gatattattt
    23521 tgtgattagg agtgtatata tgaataaaaa tggagaatgg agtgctgcac gacggatatt
    23581 cattatatta atggatgtta ttgtatacaa tgcagcaata tatagtagtt tcttactgaa
    23641 gtttcgtgga gagatcccat tacgaaattt agaaactttc caacactcag caattattat
    23701 ttcagtgatt tttattgcgt tgaacatttt gttgggtgca tatgtttttt ataatcgaat
    23761 gatcagtgac atcgtgtttg tcacagtcat cggtcaggta ttgatgacac tagggatcat
    23821 ggtagtgact tttgcgggcc gctggtttgc ttttccacgt cttgttatct tactttcatt
    23881 tgttctcggt acgcttttct taagtatcta tcgtattctc gtatataaac tttatttacg
    23941 cgtaagtcga gataaaaagg tcgtcattat tgggttagaa aaggatgtcg caccagcaat
    24001 ccaagatttt ggttcaaaga aaaacaacaa acataaagtc gaagcagtca ttcttgataa
    24061 ctattatgca aacatcaaaa atatgattaa agacatcgat attgtctatc ttgcttctca
    24121 tatcgaagaa actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg aaaaaaaatt
    24181 gtttttgaat acaacatttg aaaacttgac attgatcaat ccaaatatta tgaatttcga
    24241 agatgaaagt attattgaag catctggatt tagaatccaa cctgaatatg aactattcaa
    24301 acgtttgttt gatatcatcg tttcacttgt tttattagtt attgcttcac catttatgtt
    24361 attcacagca attttagtca aagtgacatc accgggacca atcatctata aacaagtgcg
    24421 gatcacaaaa aatcaaaagg aattttcaat ctataaattc cgctcgatga ccgccacagc
    24481 cgaagcaaaa tctggaccag ttttagctaa gagcaatgat gcacgagtaa cgcctgtagg
    24541 taaatttatt cgagctgtcc gctttgatga attaccacaa atcttcaatg tgctgaaagg
    24601 cgatatgtcg atcgtaggac cacgtcctga acgtcctttc ttcgtcgatc agttcaatgc
    24661 agaaaaccct tattactact tacggcataa tgttcgtgct ggtatcactg ggtatgcaca
    24721 agtctatgga aaatatgtct ctgattacaa cagtaaatta cgttttgatt tactttatat
    24781 caaaaaatat tcattgatgc ttgatttaaa gatcttgctg caaaccatca agatcctatt
    24841 tgataaaatg tcctctcaag gacttgaaga agacgaaaat gtgatctcat tggcagaaat
    24901 tgcttttccg aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga acaatccgaa
    24961 attcagcata atcgtaccag tatacaatgc agaagcaact attggtcgaa cgattgatat
    25021 tctaaaagaa ttggattatg aagagtacga agtcgtttta gtaaatgatg gatcaacaga
    25081 tcaaacacag gaaatcattc aagaactgat tgaaaatgat gaacgttttg aattgattac
    25141 tacgaccaat caagggcccg gattagcaag aaacgaagga ataagacagt caaaaggaga
    25201 atatctctta ttcttcgatg cagatgacac gccagtaaaa acgatcttgt cagattatga
    25261 tcatttgcta gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg tttttcgtac
    25321 aatggacgag ggaagaatcg tttccgaaaa agaaaactta gttacagaat gtcactatct
    25381 aactcacgaa gagtttataa aagacatgta tcagttaatg aatgaacagt tgatgtatgt
    25441 tgtatggaac aaatgctatc gtagcgatat tctgaaaaat aaccaaattc tatttaaaac
    25501 ttataatagt tgtgaggatc gaatatttaa cttacattat tatgaatact gtcatcaagt
    25561 gatcatgaac ccaaaagtag aatatattta tgaatttgaa ggtgggaagg ggatcactaa
    25621 tcaataccga cctaacaagt tcgaaacatt taaagaattc tattcattag caaatactgt
    25681 aacagacgat attaataaag acggtatggc ggcgcttctt ttaaaaggga caacttcagt
    25741 tattttttct atttatagta cctctgcaag aacttcaaaa gagaagaaag acgaagcaaa
    25801 gcagatattg agtgatccag tgatccgaga agccaagcaa attgctaaga cggattcttc
    25861 agcaaaaaaa ataacaaagt tgctttacaa tatgccagct ccactatttt tatctgctgt
    25921 aaaagttgga agcttcgttg aagtgaaaat gccagggtta atggctgctt tgaagcgagt
    25981 atattagtta atggaggaaa atataatgaa aagaattttg cattttcagg gacgaatggg
    26041 tttaggtgga gcagagtctt tcatgatgaa tttatatcgt aaaattgatc gtacaaacta
    26101 tcaatttgat tttttgattt atgaagatta tgcagatgtt caagactatc attcagagat
    26161 tgaaagatta ggaggccgta tttttgttgt acctaatcct aagaaaaata tttttaaata
    26221 tttaatagaa gtgaataagt tactaaaaaa agaatctttt tctatagttc acaatcaggt
    26281 ttattttggc gggggaatta atttatggtt agccaaaaag aatggaatca gacagagaat
    26341 tgctcatagc catgcaactg aagatggaaa atctcaaaat attgtaatga atgtattgcg
    26401 taaattttta accaaattgt tgttacaaaa tgcaacagac taccttgccg ttagtcaaga
    26461 agctggtgaa agtctgtttc aaaaccatcc ctttgagata gttcataatg gtattgattt
    26521 agagctttat agtaaaaatt cagaagctaa ggttaataag agaaaagaat tggatatctc
    26581 aatgagtact tttgttgtgg gaaacattgg acgattagag aaacaaaaaa atcaatctta
    26641 tttgttagag atttttttag aaatactaaa aatgaattct gattcttact tgattttagt
    26701 aggtgaaggg tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa
    26761 aagagtaaaa tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt
    26821 ttttgtaatg ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc
    26881 aaaattaaaa ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt
    26941 ggattttgta tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc
    27001 gtatgctaat aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca
    27061 aatggagagt atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta
    27121 tttttgaaaa tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg
    27181 gtcaatcgat tgaacgaaaa tgcagttttt attattcata tagataaaaa aactgatata
    27241 gaaccattta ttgaagcatt taaatcatat gataatgttg aatttttatg taataaaaac
    27301 cgtgttaaaa tttattgggg tggtttttcc attgtacaag cagaaatcaa tttagtaaag
    27361 agagcattac aaaatgagaa atatttaaaa tatgtattat tatctggtgc tgattatcca
    27421 atcaaagata atgagtatat ttataattat tttaaaaaga atagttcagt ggagtttatt
    27481 cgtggaatag atctagatca aattaaacat aaagaatttt attataaaca tatagatgta
    27541 tatcaaaaac atgattatcc tagaattaat aggaataaca caactgcatt taaaattttt
    27601 agagctataa ttaatcgttg tctgaggatg attaaacttc ccccaaaaat tagacaccat
    27661 aaatttgatt tgtatcatgg ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa
    27721 ttaatacaaa tgtatgaaca aaatcaacaa gactacctga attttaaaat aggtatgttt
    27781 gccccagatg aaaaattttt tcatacactt ttttttaatt cctcgttcaa aaataaaaat
    27841 gttattggag gaccagattt accacttgaa ttgaaaaata ttgaagaaac tactttacaa
    27901 acttcgaaat tagcaaatat tcatattatt gatccttcca tgaacaaatg gtttgatgaa
    27961 agggatatag atcaaataaa agaaagtgaa aaattatttg taagaaaagt taaatcggat
    28021 tactctgata aactattaaa tagaattgat aaagaaattt tgaaagttgg aaatatagat
    28081 gaatagaata gtacctgttt tagagaagag tttattaagt cgagatgcgt ttcgagtaat
    28141 aaatttaatc atttgtctgc tttattttat gcctagtact tttaatgtaa ctaatatccc
    28201 gatgaaaata gcttttgctt ggggtggaat tatagttttg tatgaggttt ttataaagaa
    28261 aaattttttt aaaatgcgtt tttcttggct actgattgga gcaatgatta gttttatctt
    28321 aactataatc attaacagag aaaatttttt aattccatct atttataatt ttggatattt
    28381 gctgattact ttgatagtaa tttttcctgc agacttttca caaagtgaag aggaaaaaaa
    28441 gaaaaaaatg gtgtatttta ataatgtttt cattatcata atttttttag ctgcgctaat
    28501 atctatctat caatttattt ttttgatttc ttatcatgtc ccaacaggaa cgccaggatt
    28561 acttgcaaga caaggtttta ttgaacatcg gttatttggt gtctatacaa gtccaaatgt
    28621 tggagctatg tttggatata cttctattat tttaagttta attagtgtat taatttcaaa
    28681 aataacaaaa acgattcgtg ttttttatat gatgaatttt atcgtacaaa tattttatat
    28741 tacactttct tcgtctagag gtgcccaggt agtaatttgt tcttttttag tattatttct
    28801 tttgttgttt gccaattcaa aatttagaaa gagcattaga gaagtactac ctgtaaaaat
    28861 caaatggatg gtaagcatat tattagttct attggtatat ttttcttttg gtactaatta
    28921 tgctaaggat tttttagcaa caattcctgt atcgattgat agaaaagtag aagttataga
    28981 gtcaaatgaa tcagataaaa ctccacttcc agaaaaagaa gtaatattac agcattctag
    29041 tgataactcc gaaatttcag ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat
    29101 aagaccaact ttgttatttg gttatggaga gaccgatttc tatgggaata gcgatgcaaa
    29161 atttttgaaa aaagtagatc ttgatactac agatgtgaat gaactaaaaa gagcacatgg
    29221 aaacatgcat aatggatttc ttcaagtact tgtatcaagt ggtataattg cttttatatg
    29281 tatctatgct ttctatgtgt taaatatctt tagtttactc aaaacatttt ttagaagtaa
    29341 agtgaattat gcgtttcttg gtattgtttt aatttttata ctatctattt ttatagatga
    29401 aatggtggag gctcatgtac ttt
//
LOCUS       DACCQP010000027        28601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCQP010000027 DACCQP010000000
VERSION     DACCQP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.027"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            237..1877
                     /locus_tag="IVO87_001363"
     CDS             237..1877
                     /locus_tag="IVO87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7449568.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IVO87_001364"
     CDS             2031..3353
                     /locus_tag="IVO87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7449569.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IVO87_001365"
     CDS             complement(3415..3846)
                     /locus_tag="IVO87_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7449570.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IVO87_001366"
     CDS             complement(3910..4746)
                     /locus_tag="IVO87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7449571.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IVO87_001367"
     CDS             complement(4936..5238)
                     /locus_tag="IVO87_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7449572.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVO87_001368"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IVO87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7449573.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IVO87_001369"
     CDS             complement(5692..5958)
                     /locus_tag="IVO87_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7449574.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IVO87_001370"
     CDS             complement(6212..6937)
                     /locus_tag="IVO87_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7449575.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IVO87_001371"
     tRNA            complement(7303..7373)
                     /locus_tag="IVO87_001371"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IVO87_001372"
     CDS             7490..8242
                     /locus_tag="IVO87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7449576.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IVO87_001373"
     CDS             8258..8737
                     /locus_tag="IVO87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7449577.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IVO87_001374"
     CDS             complement(8988..10568)
                     /locus_tag="IVO87_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7449578.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IVO87_001375"
     CDS             complement(10561..11304)
                     /locus_tag="IVO87_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449579.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IVO87_001376"
     CDS             complement(11480..12256)
                     /locus_tag="IVO87_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449580.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IVO87_001377"
     CDS             complement(12504..13340)
                     /locus_tag="IVO87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7449581.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IVO87_001378"
     CDS             complement(13351..14280)
                     /locus_tag="IVO87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7449582.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IVO87_001379"
     CDS             14472..14825
                     /locus_tag="IVO87_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7449583.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IVO87_001380"
     CDS             complement(14899..15555)
                     /locus_tag="IVO87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7449584.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IVO87_001381"
     CDS             15781..17715
                     /locus_tag="IVO87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7449585.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IVO87_001382"
     CDS             complement(17801..19024)
                     /locus_tag="IVO87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7449586.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IVO87_001383"
     CDS             complement(19018..20916)
                     /locus_tag="IVO87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7449587.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IVO87_001384"
     CDS             complement(20910..22028)
                     /locus_tag="IVO87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7449588.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IVO87_001385"
     CDS             complement(22111..22944)
                     /locus_tag="IVO87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7449589.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IVO87_001386"
     CDS             complement(22937..23854)
                     /locus_tag="IVO87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449590.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23877..24056)
                     /locus_tag="IVO87_001387"
     CDS             complement(23877..24056)
                     /locus_tag="IVO87_001387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449591.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24081..25205)
                     /locus_tag="IVO87_001388"
     CDS             complement(24081..25205)
                     /locus_tag="IVO87_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7449592.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="IVO87_001389"
     CDS             25388..26296
                     /locus_tag="IVO87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449593.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="IVO87_001390"
     CDS             26437..28050
                     /locus_tag="IVO87_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7449594.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8891 a         5621 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta a
//
LOCUS       DACCQP010000028        28407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCQP010000028 DACCQP010000000
VERSION     DACCQP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.028"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IVO87_001391"
     CDS             complement(451..1761)
                     /locus_tag="IVO87_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7449595.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IVO87_001392"
     CDS             complement(1773..3191)
                     /locus_tag="IVO87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7449596.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IVO87_001393"
     CDS             complement(3431..4999)
                     /locus_tag="IVO87_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7449597.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IVO87_001394"
     CDS             complement(5003..5413)
                     /locus_tag="IVO87_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7449598.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..5778)
                     /locus_tag="IVO87_001395"
                     /pseudo
     CDS             complement(5410..5778)
                     /locus_tag="IVO87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
     gene            complement(5864..6607)
                     /locus_tag="IVO87_001396"
     CDS             complement(5864..6607)
                     /locus_tag="IVO87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449599.1"
                     /translation="MTEIQIKNLIKEYEKEYIEFMEIEKLPQYKIDFFEINVEESDAA
                     GFASAAQAYYNTKTDEHILRICKSSEIPRYIVFHEFTHILDTEMYAKQDSWKYMALSG
                     YTEYHAAQVELMIMLGADSIQTQDFSFTVDVEIGNSTVRNYLNSRHQLVVNMMNRTDF
                     PRDIEALKTTVGVLYNYFGVRSICKMYAKDYTEEVDNTIIIQKLSKVLFEEINSFMVG
                     WFNEAQVELSFVSYMKIMWPMLQSYFGKE"
     gene            complement(6748..7113)
                     /locus_tag="IVO87_001397"
     CDS             complement(6748..7113)
                     /locus_tag="IVO87_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449600.1"
                     /translation="MIEENQRKSKEKIELALQAIQDMLANKERISVPKLMKKTGLSRG
                     FFYKNPTVRDTLNQAVEQQAGMIDPRREILNMAMEKQIELLNQKVAALSRENKELKRK
                     NEKLQKALRKQDLNFIKNL"
     gene            complement(7106..8605)
                     /locus_tag="IVO87_001398"
     CDS             complement(7106..8605)
                     /locus_tag="IVO87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP7449601.1"
                     /translation="MENDIIYFSDFPNLQETGTRKDNGKFDLTLLPTQELKEEFRGYI
                     MYRCKNGTFRALIQDRTAYNHIAKFLNSRINRRIKSLGDRNPEKWISLLKGWMLEQGI
                     TIVKEKKSVYGTVSYGEAVTILYFRNVLKFLGPEDLRDEIEKDVWELKNLDIKIRSNP
                     IYNVKTLDFRKIYQPDIREECKKAVYMNLQYEAIGTVQGELTIMRIFSEYLQKEYSKI
                     KSCSEIDREVLEEFLIHLSTKDTSHSANSSYVISLRRQLETIGKIYSYERLEHLFINT
                     DIPPEVNAEFRVYSDDEMKRLNAEITQMDVQIARCLLIHQMLGTRISDTLTLRPDCLT
                     RENGQDMIEIYQVKTKRYKKPISKELAKLLQSSIEYTQEKFGDTEYIFVNEKEPDRPM
                     QYMAIKTKVMSMIQEKQLKDDHGELFGFGTHMFRHYYGVKLTEMHLDDWTIARLLGHK
                     RLNNVQHYRKMSNQRMADETREVRQRMSDIIYMSLARWGEEYEQIRQDD"
     gene            complement(8595..10340)
                     /locus_tag="IVO87_001399"
     CDS             complement(8595..10340)
                     /locus_tag="IVO87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7449602.1"
                     /translation="MPIYLLQQAEHITESEVDIEKKKQELYSEIDALGIKSDSRINKL
                     KKFLADRKIWHLEEMDYPLRNSYEQYLRGQIRSQIVSFYLKIYDTVKQQHIYQQMQTL
                     NGKRIYEWKYQNKIYFLKYYPEKEIAETFETSYKTNLLVWDFTQNCSEVLKKQIFYTL
                     SRIIANTSMSKAYRNVRLKSLKLLYDSCVQLNITDIGLLEMEQVETILKNFPETSQRS
                     ILGECRRDAFMQQEQIQWEANVWYLERLHLGKHRIDESKSLISISFMEVKEIQNREIL
                     QAYMKYELGITGQAVSTIVRRFVCIRNFIELLEQEKILAIHATVAEVKKYADGLRERG
                     IQAKGFNERIFGIGHFYKFMEVKQYITRMPFRIEYFQQKEVIVHHDRSVEETVYMEIL
                     KKLYLFPERLRCMFLHLWCLGLRASEVCTLKGNAYYQQGEDYWIQVYQVKMKNYKRIP
                     IPQALYQIMKVYLKKHEIQPEEFIFKNSRGGACLYGTFRTQMIEACRENKIANGEYLF
                     QSHDYRHTVATMFYDSHVSLQSIRDYLGHTYEEMTRQYIDYMPQRIAKANDEFFEMQG
                     SSLASWLKKEEKDGK"
     gene            complement(10354..11478)
                     /locus_tag="IVO87_001400"
     CDS             complement(10354..11478)
                     /locus_tag="IVO87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7449603.1"
                     /translation="MIYKVETIKDGTEKYFFIRNLETMSIEELPSKYLMHKIKCKRSP
                     NTVKRTAFSICYYMKYMAEKEMELTEVYQLDYEKQTEHFVEFLYWLKAGNHTEQTAGE
                     KKCPNEGTCNAYLKDVFRFYLFIEAEYEQYGSLKTLSYNQIIAVNQVGVKKVLRNHSF
                     KAYLKEEEHRGRTAKENQIITILQACTNRRDQLLLLMLAETGYRIGELLGVKYVKDID
                     YRNHMIRVCFREDNENEARAKNAEYRSAKISNDTFEFLMDYLAKYRKILQHQDYLFVN
                     IMGENIGKPLRVDSVYDMLDRMEKKTGVKITPHMLRHYFANMRKQAGWELEMISQALG
                     HKHLDTTIKYLDWLDDELIEASNEFYAQHTAIYGAERLLE"
     gene            complement(11634..13601)
                     /locus_tag="IVO87_001401"
     CDS             complement(11634..13601)
                     /locus_tag="IVO87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7449604.1"
                     /translation="MYYTREYLNRAHREIDTIFRVLFPEHGMAVREEQIMLCHKMLDN
                     LLGRNIALCDAGVGIGKTYAYLVACVLMRKYSLLAEGCSPYEQRPVVISTSSIALQKA
                     ILTEYIPFLSRILQENGTIQAPIKAVIRKGKEHFVCDERLEHRIVAIEEKNKNALQKE
                     ALLSLKEHYDMDEVSNLSGFDRRMVSVPKFCSGDCPKRGSCRYQQYLERSRDHEMFIQ
                     ICNHNYLLADGYHRLQDYRPLLKDYRALIVDEAHKLPDAAKQMFGKSLCYDDIREICF
                     YLGNEYQGPEIRKLSGTIRMVLDIIGENHRTRYGIKEEFHMTEECAMYLYEGIQTMNK
                     IIEKLEKKIPKWIRNKLEETKSVLECFFHQDKKYVLHLKQDHDHRIILCASSRRIPQY
                     LDQMLWSRGMGAILTSGTLKTGQGFSHIRKMTGLQRVRRVREYVADSPFEYQKNCLLY
                     LPKNLKTCRRESQEEAEMIAGHIHSLICSTYGHTLVLFTSYHLMGNVYQMLRDEIPFP
                     MIEVWRHSPEEITRFKQMDNAVLFAAGSCWEGVDFPGDMVSSLIIVKLPFAVPDPISE
                     AQKKEYASLKDYIQAVIVPDMQKKLRQGFGRALRTETDTCVVSILDERAGEGGRYHEE
                     VMCALPSCKMAKDIKDVQHFIRNRKGVEYYL"
     gene            complement(14136..14570)
                     /locus_tag="IVO87_001402"
     CDS             complement(14136..14570)
                     /locus_tag="IVO87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7449605.1"
                     /translation="MAYNKTSEERKWNYWKEQEEKKLRELGMEEEKIQKLRQMDREDF
                     LEERRYREHLDETMQDMDSIKEKDSELFARDVKELLDHIGDKRIYELLKETEHQTLEI
                     LLLSTWGYSGREVAKIMGMAEQTIYTKRNRLRKKLKNLSKIE"
     gene            complement(14898..15524)
                     /locus_tag="IVO87_001403"
     CDS             complement(14898..15524)
                     /locus_tag="IVO87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449606.1"
                     /translation="MQKKNCVLCISIGIIVCILLSACSRQPDFDAKSYVQSSLDAHYH
                     GEYKDYANLLEISEKDAKKEIEEDFNESIQQQFDDSDNITDKGIADYAEKLTEVKKLA
                     KYKVQDVKEEDGVYTVSVQVEPSNVFQTLQQSSTEVSNEKIKQGLDGNDPEVFAAVLT
                     ESVQKSLEKNRYGKAVTVNVSVEKDNSGKYGLLDTEMNKLEAAMFPTE"
     gene            complement(15977..16402)
                     /locus_tag="IVO87_001404"
                     /pseudo
     CDS             complement(15977..16402)
                     /locus_tag="IVO87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
     gene            complement(16955..25825)
                     /locus_tag="IVO87_001405"
                     /pseudo
     CDS             complement(16955..25825)
                     /locus_tag="IVO87_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
     gene            complement(26017..27291)
                     /locus_tag="IVO87_001406"
     CDS             complement(26017..27291)
                     /locus_tag="IVO87_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7449607.1"
                     /translation="MNIHLITYLTSKLFWIYTVFRYNTILYPKNEDTQKLFAYFCAYL
                     LSCWGSMQWGTMYGDLIFELIALLIITVAYQADWQHHVWNTLMVYIMGILCDGIPIVF
                     FQRYAVARNPVHHHMGPGTIVMGNFIFFLLEVIIERKVGVKRSYYIKNRHWLAMLSVP
                     VISIIVCIRILLPDRRGVRSTIVLFLLFINVFIFYLHDEVQKSYIETAKQEEMKIQMS
                     QYAKELEMMMKSQDRLNKIHHDYKHHLTTIGAMAKTGGNEEILSYLKQMENIAGTALC
                     HHLYTENRNMNNLLNYILEDSKSVIENPEISVEIPNHIGEELFDISIIVGNLMDNAIR
                     GTAASDERRLSFQMYYGKGIMNIQIENSIKDTPKVRNGIYLTTKSRKEGHGIGLQNVK
                     LVVEKYHGQMEICHTEKSFQVKILLYMKLDEK"
     gene            complement(27288..28001)
                     /locus_tag="IVO87_001407"
     CDS             complement(27288..28001)
                     /locus_tag="IVO87_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449608.1"
                     /translation="MLELAICDDDIILCNWLEQKLLHYGKANDCQISIEIYYSAEQLL
                     NRLEEESYDMLFLDICLEHENGIDIGTEIRKKNYGKELRITYISTYQEYAMKLFRIHP
                     FDFLVKPIREEELFRVIGELRELLKERKKIFEYTDTKGIHKISYDKILYFYSLGKRVH
                     IVTSEKEYEYRGKLKDVADVTEEYFLLIYKSYLINMEHVEHYEYDNVVMKDKTRLSIS
                     KANRKKVRERLLQTEKKRL"
BASE COUNT         7361 a         6414 c         4488 g        10144 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgc agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaataggg aactaaaatg tagccagttc ccctcatgcg ttgattttac
     5821 tgggtttaac tgtatacaaa actgtgttta ctgttactat ctttcattct tttccaaaat
     5881 agctttgaag cataggccac attattttca tatatgacac aaagctcagc tctacttgtg
     5941 cttcattaaa ccatccaacc ataaagctat ttatctcttc aaataatact tttgaaagtt
     6001 tctgaataat aattgtgtta tccacttctt cagtataatc tttcgcatac attttacata
     6061 tagaacgcac tccaaaataa ttatatagca cacctaccgt tgtttttaat gcttcaatat
     6121 ctcttggaaa atctgttcta ttcatcatat tcactactaa ttgatgtctt gagtttaggt
     6181 aattcctaac tgtactatta cctatttcaa catcaactgt aaatgaaaaa tcttgagttt
     6241 gtatactatc tgctcctaac attatcatca attcaacttg tgctgcatga tattctgtat
     6301 aaccagacaa agccatatat ttccaagaat cttgctttgc atacatttcc gtatctaaaa
     6361 tatgcgtaaa ttcatgaaac acaatatacc ttggtatttc cgaacttttg catatcctta
     6421 atatatgttc atctgttttc gtattatagt aagcttgggc ggctgaagcg aatcctgctg
     6481 catcgctttc ctcaacatta atttcaaaaa aatctatctt atactgtggt aatttctcaa
     6541 tttccataaa ctcaatatat tctttttcat attctttaat taaattttta atttgtattt
     6601 ctgtcatatt gttttatacc tcatatcaaa gttagcattt cacttttagt ataccataaa
     6661 acaatcccat tctcattcta tatattttat cctcaacgat gaaaagatgt gtctgcacac
     6721 atctcttcca tcttattatt ttcaatgtta taagttctta ataaaattca aatcctgttt
     6781 ccgtaatgcc ttctgtaact tttcgttctt tctcttcagt tccttatttt ctctgctcag
     6841 agctgccacc ttctggttca acagttcaat ctgtttttcc atcgccatgt tcagaatttc
     6901 tcttcttgga tctatcattc cggcctgttg ctctactgct tggttcaatg tatcccggac
     6961 ggttggattt ttataaaaga atcctctcga caatccagtt ttcttcatca gctttggaac
     7021 gctgatacgt tctttatttg ccagcatatc ttgtattgct tgcaatgcca gttcaatttt
     7081 ctctttactt tttctctgat tttcctcaat catcttgtcg tatttgctca tattcttcac
     7141 cccatcttgc cagactcata tatatgatgt cactcatcct ctgtcttact tctctggttt
     7201 cgtctgccat tctttgattg ctcattttcc ggtaatgctg tacattattc agtcttttat
     7261 gccccaatag tctcgctatc gtccagtcat ccagatgcat ttctgtaagt ttgacgccat
     7321 agtagtgccg gaacatgtgc gttccaaacc cgaataattc gccatgatca tctttcaact
     7381 gcttttcctg tatcatggac atgacttttg tttttatagc catatactgc atcgggcggt
     7441 caggttcttt ttcattaaca aatatgtatt ctgtatcacc gaacttttcc tgtgtatatt
     7501 caatagaact ctgtaacagt tttgcaagtt ctttactgat cggtttttta tatcttttag
     7561 ttttaacttg atatatttca atcatatctt ggccattttc tctcgtcaga caatctggtc
     7621 tcagtgtaag ggtatctgat atcctagttc caagcatctg atgtatcaga agacatcttg
     7681 caatctgtac atccatctgt gtaatttccg cattcagtcg tttcatttca tcatcagaat
     7741 acactctaaa ttctgcattt acttccggtg gaatatccgt attaataaat aaatgctcca
     7801 atctttcata gctgtaaatt tttccgattg tctctaactg tcgtcttaat gaaattacat
     7861 aagaactatt cgcactatgg gaagtatctt ttgttgacag atgtatcaaa aattcttcca
     7921 acacttctct gtctatttca ctgcagcttt ttattttgga atactctttt tggagatatt
     7981 ctgagaatat cctcattatt gtcaattctc cctgtacagt tcctattgct tcatactgca
     8041 gattcatata aacagctttc ttacattctt ccctgatatc tggctgatat atttttctga
     8101 aatccagtgt ttttacatta taaattggat tggatctgat tttaatatcc agatttttta
     8161 gttcccaaac atctttttct atctcatccc gtaaatcttc tggccctaaa aatttcagta
     8221 cgtttctgaa atataatatc gtcactgctt cgccatagct tactgtacca tacacactct
     8281 tcttttcttt tacaatagtt attccctgtt ctaacatcca gccttttaaa agtgatatcc
     8341 atttttctgg atttctatct ccaaggcttt tgattctccg atttattctg ctgttcaaaa
     8401 actttgcaat atgattataa gctgttcgat cctgaatcaa tgctctaaac gttccgtttt
     8461 tgcagcgata cataatataa cctcgaaact cttcctttaa ttcttgcgtt ggaagcaacg
     8521 ttaaatcaaa ttttccatta tccttacgtg tccctgtttc ttgtaaattg gggaaatcgc
     8581 tgaagtatat aatatcattt tccatctttt tcctctttct ttagccatga tgctaaacta
     8641 cttccttgca tttcaaagaa ctcatcattt gcttttgcaa tcctttgcgg catataatca
     8701 atatactgtc ttgtcatctc ttcataagta tgtccaagat aatccctgat actttgcagt
     8761 gatacatgac tgtcataaaa catagttgct actgtatgtc gataatcgtg tgattgaaat
     8821 aaatattctc catttgcaat cttattttcc cggcacgctt ctatcatctg agttcggaat
     8881 gtaccatata gacaagcacc acctctgctg tttttaaaaa tgaattcttc aggctgaatt
     8941 tcatgttttt tcaagtatac tttcataatc tgataaagtg cctgtgggat tggaatcctt
     9001 ttataattct tcattttgac ctgatacacc tgaatccaat aatcctcgcc ctgctgataa
     9061 taagcatttc ctttcaatgt acacacttca ctcgcccgta atcctagaca ccacagatgt
     9121 aggaacatac atcgtaatcg ctctggaaaa agatataatt ttttcaaaat ttccatatat
     9181 actgtttcct caacgctacg atcatgatgt actattactt ctttttgttg gaaatattca
     9241 attcggaatg gcattcttgt aatatactgt ttaacttcca tgaatttata aaaatgaccg
     9301 ataccgaaaa ttctttcatt aaatcccttt gcctgtattc cccgttctcg caagccgtct
     9361 gcatactttt ttacttctgc gacagttgca tgaatcgcca gtatcttttc ttgttccagc
     9421 agttcaataa aatttctaat acatacaaat cttcgtacaa tcgtgctgac tgcctgtcca
     9481 gtaattccca gttcatattt catgtatgcc tgtaaaattt ctcgattctg tatttccttg
     9541 acttccataa aagaaatgct aattaaggat ttactttcgt ctatccgatg ttttccaaga
     9601 tgtaatcgtt ccagatacca tacatttgct tcccattgga tctgttcctg ctgcataaac
     9661 gcatctcttc tgcattcacc taaaatactt cgttgactcg tttcgggaaa attctttaaa
     9721 atagtttcca cttgttccat ttccagcaat ccaatatccg taatatttaa ctgaacacaa
     9781 gaatcataaa gaagttttaa ggattttaac ctgacatttc tataagcctt agacattgaa
     9841 gtattggcaa tgatacgact caacgtataa aaaatttgtt tctttaaaac ttctgaacaa
     9901 ttttgtgtaa aatcccacac caataggttt gttttataag aagtttcaaa ggtctcagct
     9961 atttcctttt ccggataata cttcagaaaa taaattttat tttgatattt ccattcatat
    10021 atccgctttc catttagcgt ctgcatttgt tgatagatat gttgttgttt taccgtatca
    10081 tatattttca aatagaaaga tacaatttgt gatcgtatct gtcctcttaa atattgttca
    10141 tacgaatttc gcaaaggata atccatttct tccagatgcc agatttttct atctgccaag
    10201 aactttttca atttgttaat tcttgaatcg ctttttattc caagtgcatc aatttctgaa
    10261 tatagttcct gctttttctt ctcaatatct acttcagatt ctgttatgtg ttctgcctgt
    10321 tgcaataaat atataggtat ttctcacacc tccctactcc agcagacgtt ccgccccata
    10381 aatagcagtg tgttgtgcat aaaactcatt acttgcttct atcagttcat catccagcca
    10441 gtccagatac tttatagtcg tatccaaatg tttatgcccc aatgcctgac tgatcatttc
    10501 cagttcccat cctgcctgct ttcgcatatt tgcaaaatag tgacgtaaca tgtggggtgt
    10561 gattttaact ccggttttct tttccattcg gtccaacata tcatatacac tgtccactct
    10621 taatggcttt ccaatgttct cacccatgat attcacaaac aggtaatcct gatgttgcag
    10681 gattttccga tattttgcca gataatccat gagaaattca aacgtatcat tacttatctt
    10741 ggcacttctg tattccgcat ttttggctct cgcttcattt tcattatctt cccggaagca
    10801 tacccttatc atgtgattcc tataatcaat atctttgaca tattttactc ccagtagttc
    10861 cccaattctg tatccggttt ctgctaacat aagtaacaga agctgatctc ttcggttcgt
    10921 acaggcctgt aaaattgtaa taatctgatt ttcttttgct gttctcccac gatgttcctc
    10981 ttccttcaga taggctttaa aagaatgatt ccgcagtacc ttttttactc ccacctgatt
    11041 aaccgctata atctggttat acgataaggt tttgagactt ccgtactgtt catattctgc
    11101 ctctataaat aaatagaacc tgaacacatc ttttaagtat gcgttgcagg ttccttcatt
    11161 cggacacttc ttttctccag ccgtttgctc tgtatggttt ccggctttaa gccagtataa
    11221 aaattccaca aagtgttctg tctgtttttc ataatctaac tgatagactt ctgtgagttc
    11281 catttctttc tctgccatat atttcatata atagcagatt gaaaatgctg tgcgtttcac
    11341 tgtatttggt gaacgtttac atttaatctt atgcattaga tatttacttg gcagttcttc
    11401 aatactcatt gtttccagat ttctgatgaa aaaatatttt tctgttccat cttttatcgt
    11461 ttccaccttg tatatcataa gctgcctcct ttcctctaac gctatacaca gtatacccac
    11521 ttcatgccga aatagctatt catcaatacc aacaatccca ccgaaaacgc ccttgaaata
    11581 ccaccaaact tttcgtgcaa aatctgcttt ttctgtagca gaaccacgaa cggttacagg
    11641 tagtattcca cgcctttccg gtttcgaata aagtgctgga catccttgat atcttttgcc
    11701 atcttgcagc tcggaagcgc acacattact tcctcatgat acctgccacc ttctcctgca
    11761 cgctcgtcta aaatggacac gacacaagta tctgtctcgg tgcggagagc acgtccgaat
    11821 ccctgtctta gttttttctg catgtctgga acaatcactg cctgaatata atctttcagg
    11881 ctggcatatt ccttcttctg tgcttcactg atgggatcag gaacagcaaa gggcagtttt
    11941 actataatca gcgaagatac catatctcca gggaagtcta ctccttccca acaggagccg
    12001 gctgcaaaaa ggacagcatt gtccatctgt ttaaatcttg tgatctcttc cggtgaatgt
    12061 ctccacacct ctatcatcgg gaacggaatt tcatccctta acatctggta aacgtttccc
    12121 atcaaatgat aagatgtaaa cagcactagc gtatgcccgt aagtcgaaca gatcagtgaa
    12181 tggatatggc ctgctatcat ctctgcctct tcctgacttt ctcttctgca tgtcttcaaa
    12241 ttctttggca gatataacag acagttcttc tgatattcaa acggggagtc tgccacatat
    12301 tcccggactc tccgcactct ctgtaatcct gtcattttcc gaatgtggga aaatccctgt
    12361 ccggttttta aagttccgct tgtcagaatc gctcccattc ctctgctcca taacatctgg
    12421 tccagatact gtggaatccg tctgctggat gcacacaata taatccggtg gtcatggtcc
    12481 tgcttcagat gcagcacata cttcttatcc tgatggaaaa agcattccag cacacttttg
    12541 gtttcttcca gcttgtttct gatccacttt ggaatcttct tttctaattt ttctatgatc
    12601 ttattcatgg tctgtatccc ctcatataaa tacattgcac actcttctgt catgtggaat
    12661 tcttctttta tcccatatct ggttctgtgg ttttctccta tgatgtccag caccatccgg
    12721 atcgtcccgg acagttttct tatctccggg ccttggtact cattcccaag ataaaagcag
    12781 atttctcgga tatcgtcata acagaggctc ttcccaaaca tctgctttgc cgcatccggc
    12841 agcttatgtg cttcatcgac aatcaatgct cggtagtctt ttaataacgg gcggtaatcc
    12901 tgcagcctgt gataaccatc agccagcaga taattgtgat tacagatctg aatgaacatt
    12961 tcatgatctc tggaacgctc cagatactgc tgatacctgc atgagcctct tttcgggcag
    13021 tctcccgaac agaacttcgg tacactcacc atccttctgt caaacccact taagttggac
    13081 acttcgtcca tatcataatg ttctttcaac gacagcagag cttctttctg caacgcattt
    13141 ttgttttttt cttcgattgc aacgattcgg tgctccaacc tctcatcgca gacaaaatgc
    13201 tctttccctt ttcgtatcac agctttaata ggggcctgaa tcgtcccgtt ctcctgaaga
    13261 atccgggaga gaaaagggat atattctgta agaattgctt tctggagtgc aatgctggaa
    13321 gtggatatca cgaccggacg ctgctcataa ggggaacatc cttctgcaag gagggaatat
    13381 tttctcatca gaacacaggc caccagatag gcatacgtct tgccgatacc gacacccgcg
    13441 tcacacagtg ctatgtttct tcccagcagg ttatccagca ttttgtggca taacataatc
    13501 tgttcttccc ttactgccat tccatgttcc gggaacagca ctcggaaaat agtatcaatt
    13561 tctcgatgcg ctctattgag atattctctt gtgtaataca taatttgtta cctgatatgc
    13621 gtgctgatat cacatcaata cggctgtaaa ggatttctcc ctacagccgt atgtatctga
    13681 tatcaacgac acattcttcc ttgatttctt atacttttga tttttggcgt tgtatttgtc
    13741 ctcgactccc ccattgcctt acgggaacat tacaggcgga tgcttggcat tgcaccgctc
    13801 acggattttc taaaagtctt gctgattaca acccaaagtc catcctctgc tcactggcag
    13861 ttcctatgta tcattatccc ctcgtaagtg atcctcgcaa aagaccgcca cggtcctgtt
    13921 tcagttaaag atgattcgct cgaaacgaaa acgccacaaa agaactctgc ctctcacttt
    13981 ctggtctaat ttcttccatg cccttttcat ctgtcctcgc gtcttcctgc cacgcttcta
    14041 cttacgggaa cgtacctgta atgctggtat gaggttgtca aagtacactg gcaaaattta
    14101 cctgcctatt aaatagccga cgaaaaacgc cataattatt caatttttga taaatttttt
    14161 aatttttttc tcaaacggtt tcttttggta taaatcgtct gttctgccat tcccataatc
    14221 ttagcaactt ctcttccaga atatccccat gtactgagta agaggatttc cagtgtctga
    14281 tgctccgttt ctttcaatag ttcatagatt cgcttatcac ctatgtgatc cagaagctcc
    14341 tttacatccc ttgcaaacag ctcgctgtcc ttttctttta tggaatccat atcctgcatc
    14401 gtttcatcca gatgttcccg gtatctgcgc tcttccagaa agtcttccct gtccatctgg
    14461 cgcagctttt ggatcttctc ttcttccatt cccagttcac gcaatttctt ttcttcctgt
    14521 tctttccaat aattccactt tcgttcttca cttgtcttgt tataagccat ttatctttcc
    14581 tccgatctga tttttgatat agaaatcaaa tcggagcatg gcggtgaccg gataggtacg
    14641 ttataatgtt cgaaaaatgt cgaagaaaaa aatgctgcaa cctcttcgat tacagcactt
    14701 ttttccatct tacgtgaata tttcttgttg gttcatagga gattccttct ttttctcata
    14761 atgaataaaa gactcttgtc atccattatt tattataatt tttctactat tacaatagct
    14821 tttttaacaa gttttcttgt cttggagttt aaaagaaagc taccagtttt tttcctctga
    14881 tagctttcca ttcattctta ttctgttggg aacatcgctg cttccaactt attcatctct
    14941 gtatccaaaa gaccatattt tcctgagtta tctttttcca cggatacgtt tacagtaaca
    15001 gcttttccat atctgttctt ttcaagactc ttctggacac tttctgttaa tacagctgca
    15061 aagacttccg gatcatttcc atccaatccc tgctttattt tttcattgct tacctctgta
    15121 gagctttgtt gcaatgtctg aaatacattg gatggttcaa cttgtacact tactgtatag
    15181 actccatcct cttctttcac atcttgtact ttgtattttg ccagtttctt tacttctgtc
    15241 agtttttccg cataatcagc aattccttta tccgtaatgt tatcggaatc atcaaattgc
    15301 tgttggatac tctcattaaa atcctcttcg atttctttct tggcatcttt ttccgagatt
    15361 tctaacagat tcgcatagtc cttatattct ccatgatagt gtgcatcaag agaactttgg
    15421 acgtaggatt tggcatcaaa gtctggttgt ctggagcaag ctgataacaa tatacatact
    15481 ataattccta tacttatgca caaaacacaa ttcttctttt gcatattctc tccttcacgc
    15541 tttatttatc ttcaattttt atcgaaaata acatttttaa atactgttct attgactcct
    15601 tttgaacttt tctttcaata ttatatggaa tgcacataaa aaactccaca taaccattat
    15661 caataaaact cagcaatact tctccatttt ctgttaaaaa atgtacatat tttttattac
    15721 atttactttc aaatttaata ttcgacattt gcttgtatat ttttttgtaa tgatttttta
    15781 tgcttacttc atctgtacaa tcagttccat cacctatcaa aaaagaaata tacaacttat
    15841 cataatcttc atttctatat gtcatataag aatctgtatg accatcacca atatttttat
    15901 aatgctcatt tggaatcgaa taactaatta ttttcccctc taatgtttca aatctttctg
    15961 tttcatcaat tctaatttca catggttcaa acattttttt tataaacaag cccaattcat
    16021 aatccgacat ttctataaaa ttaatataat ctttttcttc cgtttttcgc gaatatcccc
    16081 ctctattatt ccgttcccat cttctaattt ctaattcatc tccattttgt ttcaaatcaa
    16141 ttctttgata attcttggaa aatgttggaa aacttttata ttttgtttgt ttccaaaaat
    16201 tgtttaattg agattcgtct acttttgggg aactttctgc aatttttatt ccttttctaa
    16261 ttgcacagcc tatcttatct attgtttctt cagccataat ttcaattgta ggttcgatag
    16321 aaatataaca tcccataaac ctttcaatgg cattaggcac aattaagaaa ccatattttt
    16381 gattataata tacagaaatc atatccgttt tctcctctct attacttaag gggaataaaa
    16441 tattcccctt aagctattta ctttgctatt attttcctac tgtaataatg agtttaacac
    16501 ctttatcaat accatgtttt gtagcattat taactgcatt tatctgagct tcgcttaatg
    16561 ttacatttgg aacaactact tgtaattgtc tacccactat ttcttccgca ttaacaaatt
    16621 taccattaaa tgtttctcct ataaatcctg aaactttatt aatgtccttg acaattacct
    16681 tttccaattt attaccattt tgataagtct ttgcacctaa gtctcggctt ttaacactag
    16741 tcacaacacc attttctact ttatctatgg tcgggaaatt gtgtcccaag ttatttccca
    16801 tagctttgtc aataatatct cctcgctcta atggtggttt ttcccaagtg ccttctggca
    16861 attttgtttc ggttacttct gcaactaatt ttttaaacga tttatcaatc ccatcattat
    16921 gggtacttcc tgcactcctt actacattca aaagtttccc agccccataa ctaaggccac
    16981 ccataacgcc acctgtcact gctcctgcaa taccaccgac taacgtatct tttgctactt
    17041 gtttcagact atttccagac aaacctgaat tcactgcact atatgaagct ccaccaatag
    17101 ctccaataac tgctcctgaa accatcgccg ttcctaagcc acccattgct ggcaatgctg
    17161 ccagaactgc tcctgcagcc aaaccgcctg ttgcagcaac tacaagacca gaaactgtaa
    17221 ccgcagttat accaatggct acttttttca ccgcattcca gtctactttt ccaagcttat
    17281 ctgtaatacg atttgttgtc gtacacatat gtcgtaccac ttctgagcgg atttctgccg
    17341 ttctttctga aacataggat ttgaaagatg tataagtttt tccaattttc tgtaccccat
    17401 tgcttacaga acgaataaac tgacttcctc tggatgtgat cttttgatat gtactgctac
    17461 tggttacacg atttttcaca gattgatatg tctgttttgc atgtttagct gcatttttgg
    17521 cagtttttgc tacccccttc actgtgttaa caatagtttt tgctgctttc ttagctgtat
    17581 ttactactgt tttgactacc tttttcgcag tattaacgat tttcttgccg acactcttga
    17641 ctgctttcgc tgctttttta gctgtactct taatcctgct ccacaatgaa tgtcctgtcg
    17701 gatcactgta attcagcgga ttattggtcg tataatcgta gcggtttctg gtcaatggat
    17761 tttctgtcgt tccaaggtca ctatcttcag ttgtaaaggt tccgttctct gcgttatagt
    17821 atcttgcccg cagatactga agtcctgttt ttacatttgt ggactcccca ttgtaaccgt
    17881 agtaattgac accgtctgct gttcctgatg taaggttacc gtatgaatca tactgataac
    17941 tgttcgtcag attagctgtt tcggtcagga ttccggtcac gctgtttctt ccgtcatata
    18001 aataataact gctttcttcc gatttatcaa cgcttacccg ttctccgatt ccactttctc
    18061 cataagtata agcctgacgt actttctcgt ctgctccata ctctgcaagg acttctgtat
    18121 tctcgcgatt gatatcgttg atatactctg tcagtgtgta tcctttggca ttggaaccgc
    18181 cttttaccag agaggcaagc tgctctttcg ggctgtttcc atcttcggta cgctggttcg
    18241 ccgggatcag gacttcatcg ccatagcagt cttcccattt ataggtgtta tcaagttcga
    18301 atacccggtt gttatctcca tcgtacatgg ctgccatcag gactgttcca ccctgtttta
    18361 ctactgccaa acgattctct gctgtgtatt cataagtaac cggatctgca ctgtttgtct
    18421 tatcacattc ctggatcaga ttgccatctt tatcatagct gtaggttgtt cccggatcgc
    18481 cccagatctt tgcattggtg cgtttgatga gctggttgga atcgttgtac ttatagttat
    18541 attttgcttt gctgacacca tcctcgatct tctcgtaaga ggtccggttt ccaatcttgt
    18601 cataagtata attatggact gtctttttac cgccctctgc tttttctgta cagcgtgtca
    18661 gttcccagtt gtcatcgtat tcataggtac gtgtcgtctg gatctctttt tcctgatgct
    18721 cacagtctgc tttgtctgtc tctttctgtg tatcgcccca gttgtaccag tcttcccagc
    18781 ttggcgtctt tcttgttcca gcttccagtt ccgtagctgt ttctccaact acatatccct
    18841 gatcattgta cttgtactca taagtactga ttactttgcc gcaggaacca caggtattca
    18901 caatctttgt gatatgatct tctgcatcgt aagatacttc cgtctttgtt ccattacttc
    18961 tagtaacttc tgtcacacgg ttcagtgcat catgcttgta agtggttact tttctattac
    19021 ggtctgtcag ttttaccaga ttatcattta agtcgtattc atagctgatc tttgtcccat
    19081 ccgggtatac gattgcctgc agattatctg cgtcatcata aacgtagctg acatcctttt
    19141 tcgaaccgct tgtcactttg gtgattcttc caagtgcatc gtattcgtag ctgctcttgc
    19201 ctgtctgatc cttcatggat acccgttccc cggcactgtt atatgcgtag gtcacgtcct
    19261 tttcggatgt ctcgcctttt gcgtcctgat aagatttctc caacaggtca ttcagtttgt
    19321 cgtaatcata cgtggttttc ttgccgcttg cctgtgtatg tccggtcagt ctgccttttt
    19381 catcatagtc gaatttctcc gttcttcctg ccggatcttt gatggatgtc aggttgcctt
    19441 ctaagtcata agtataattc gttgtcttat ttgctgcatt ggtgaaggtc accaggttat
    19501 ccatgttgtc gtaaccataa gttgttacac cgccaagggc atcagttact ttggtcaggt
    19561 tgtcattctt gtcatattcc agatggctct ttaatccggt cttatctgta acaacctgaa
    19621 tattcccatg tgcatcgtaa tcaaatcccg ttgtgaatcc acgcttgtct gtaatagaag
    19681 ttaccctgct gtccttatca taggtatagg attccttctg ccccagtgca tcggtctctt
    19741 ctgttacacg gttgtcttta tcgtagactg tctttgcaac atagcccatc ggatctgtta
    19801 cagatgtaac attataattc ttatcatatc catagctggt ctttcttcca cccggtttgg
    19861 tgatctcagt aatatttcct gccgggtcgt aagtgtaagc agttatctta ccaagcggat
    19921 cagctgcaga agtcatctga cctgccaggt cataactgta ggtattgctc cgtccaagcg
    19981 gatctgtctc cttcaccaga ttgccatact tatcataaga aaagctggtt gttccatcca
    20041 gtgcattggt agaagacttc atgttatgga gcttatcgta agtataagta gtggtactct
    20101 ttaagctgtc gctttctttt accacgttgt ccactttatc ataagagaaa tttttagtat
    20161 aacccagtgg actggtcata ctcttcagac gtccggcttt atcataacta taggtatact
    20221 ctccaccatt cgcctgtgtc atctttgtga cattgtcatt caggtcaaca ctgtactgtg
    20281 tctcatttcc ctttgcatcc gttacagaaa tgacattgcc atgcttgtcg taatcatatt
    20341 tggtaactgc tccctttgga gaaatctgtt ccgtcatgtt actcaggctg tcatatttgt
    20401 attcggtaat gagtcccatc ggatcttttg atttggtaag atttccaatg gcatcataac
    20461 ggtattcatt tgtttctttt aacggagaag tctcaccgat cttgcggtcc agttcatcat
    20521 actggtaggt tgtcgtattt ccatttccgt ctgtctggga tacgagattg tcatttttat
    20581 cgtaagcata cttggtaacc gtaccgtttt catcgacaga ttccgtcaca ttatcatttc
    20641 cgtcatactt aaaggtgctg gatgctccca gtggatttgt ctgagaaata acccggtctt
    20701 tcttatcgta agcgtattta tatgtggctt cctcttcttc tgtcatggat aactggttgc
    20761 cgactttatc gtaggtaaag gttgttttat gaccttccgc atccgttacc gctgtaatgc
    20821 ggtccataac atcatattca taagatgtga tacttccgtc tgcttcagta agtttcagga
    20881 gtctgccgta ctgatcgtac ttgtatgttg ctgtcctgcc aagttcatcg gtctgtgtca
    20941 gtacatttcc ggcaccatca taagtataat ccatactgtt tccaccattg tcggaggaat
    21001 ggatcaggtt gcctgctccg tcatattgat aggtaattac gagtccctgt gcatccgtac
    21061 attttacaag ctgtccgatg gcatcgtact ccatcagcac cttgtcccct gtcggcagta
    21121 ttatggatgt gttatttccc tctttatcat acacataact atacgcattg ccgagggcat
    21181 ctgtcatctt tgtgctgttg ccctgtgaat cgtattcgta agtggtcttt cctttcaggg
    21241 catccaccgt ctgttccaga ttgtcggtgg aatcatattt cattgctgta atgttgccct
    21301 ttgcatcgct taccgatgtc atgtttccat taccatcgta agtcatggta ctggtatttc
    21361 cattttcatc ggtagaggat accagatttc catttaagtc atacttcatg gatacggtat
    21421 atccaagggc atctttggat gtagtcagat ttcctgcctt gtcgtaagca taagtatctg
    21481 ttcttccctc ttcatcggta gaagatacca gatttcctgc tttatcataa gtatatgtgg
    21541 tagttccacc ttctgggttg gtttcactta agcggtttcc tgtattatcg taggtataag
    21601 tccaggttct tccactgtca tctgattttg ataccagatt gtctgccgca tcataagtat
    21661 aagtggtaac tgcacctaat tcatctgtct ctttagcgag tcttccgcag ttatcatatt
    21721 ccttggaatg gctgcttcca tctccataag taatcttggt ttcatttcct aatgcgtcat
    21781 attcatattt ggttatatta ccaactgcat ctttggactg aagcacctgt ccaagagaat
    21841 caaacttgga tacggttgca tttccatttc catcggtata ttttttcaga tttcctgctt
    21901 tgtcatattc atagagcttt gttccaaaat ctttattact ctcttctatg acacgtccca
    21961 tgctgtcata cttataagta attgtatttc cttttgcatc ggttgctttg acctgctgat
    22021 aattcttatc ataaacgtag ctgatctgat ttcctttcgg atctgtacca ctggtcatgt
    22081 tgtacatctt atcataagta taagtggtgg tattccccag tgcgtcagta cttgctgtaa
    22141 tatttcccac accatccagc gtataagcgg tcacaccgcc atcggctgct gtcttggaag
    22201 tcagattgcc attcgcatcg taactgttcg aggttacatt tccaagtgga tctgtcatgg
    22261 taactgcacg attcatcgcg tcatacgtga atccccatgc tgctccattt ccatccacat
    22321 atccggtcat gttcggtccg tcatacgaat acgttgttgt gacacctctg ccgtctgtct
    22381 gggataccat acgatgtttg tcatcgtaag tataggtaat ggctgttcca tccggttttg
    22441 tagatgtgac catatttcca ttaccgtcat aactgtaagt aaccgttgct ccgttatatc
    22501 cagttgcact ggtcagctta ttctgctcat tataagtata agaggctgtc tttccatcct
    22561 ctcttgtctc cgttgctaca ttgccaaatg tatcataagt gtaagttttt gttccggctg
    22621 ctgtcgtttc ggaagcaagc tgattctctg cattataagt cttttcacag gttgttccat
    22681 ccggatattc aatggatgtt gtgcggtact gatcatcata atgataaacg gtcttatttc
    22741 cttcgttatc cgttgttgta gtagaactct ttccgtactt gaaagttgcc gttccttttt
    22801 ctgcatcagt ctgctctaca actcttcctt ctttatcgta agtattcttt acaaccgtat
    22861 tgccattctc atctttccag cttgtcatac ggctgtcatc atcatatttg tactcttttg
    22921 ttgttccatt cggatctgtt acagatatca gatttccctg ctcatcatat ttgtaagaaa
    22981 cttttccacc atctggaaga gacagttcct tgatatgccc gaatttatcc tgttttaccc
    23041 cgaatgtttt tccggatggt gttgtaatct tactcagatt ataatcatcg tcataatcta
    23101 atactgtctt aaatccattc acatccgtta cataacgtaa aattccatat ttatcaaatg
    23161 accagacgct ctgatccgca tctgtcagtt cccatccgat gtagtcatgg tctgtatctt
    23221 tatagctgac tgcttttaaa tcatatacat aaccatccgg agtagtgtag cttccatctt
    23281 cattcttatc aaagatcaga taacttccgt cccctctcat atacagaaga cttccgtcct
    23341 ccatttgaga aagtgactgg tcatagttaa agctccatcc acgtccgaac atggaatgct
    23401 ggtctgttcc tttggaattg tagcttcttg taatggagaa ttctccattc agttcattca
    23461 tcgtcgcatc ggactgatcc atgtagaagt taccagtatt taagttgact ggttcaaatc
    23521 caaattcaca gtgcaggcct cttcccatca atgctccgtc aatccgcatc ttatcctgat
    23581 cggtaagcgg tgcaggacta tatggaacat ttgtctgtgg gttacggata aagatcgtgt
    23641 tatttgcgac taccagtgcg tccataacct gattgtcttt cataatgttc ttaagcggaa
    23701 caccataata ttttgcaatc ttcgggaatg tatcaaactg cttcacttca tagatcacaa
    23761 agctgtcact ggtcacttct tttcctgttt ccagctttcc atcaatctct ttcgatgctt
    23821 ttgctttgat cttataaagc ttgtccttcg tcagccctga atacagtgca ctctgccagt
    23881 tggaatcttt gctgtaatac ttattcgcct ccgggaactg cttattgtat tcagtactgt
    23941 ctggatagga atacaatggt tttgcatctg tttcgtgatg ttcttcattt tcctcatctg
    24001 gaagcagata atattctact gtagatttgc tctttgcaat tccgtctgca aatacggcat
    24061 caaacttcag ctttccattt gtattctttt ctgtcatcgg gcgaagcagg atcgttgttt
    24121 catccagtga catatctcgc agatatggat cttcagccgg tgaccactgg atgctgagtt
    24181 tcggtccata aacggatgcc ctgttattca gtacctcaca ctgcatagaa gctccaaggt
    24241 tatttgcctc ggcaatggct accagtgcaa atccgttatt cgcatgagtt ccctgcaccc
    24301 agtcattgac cagatctttg acgtcataat tgatatagct gttcctgctg gtacttgcat
    24361 tctgtacatc ctgaaacgta agatttactg gttttgtttt ccatgtgatg gaggtgttcc
    24421 acgactgatc tacacgataa agtccaaact gggatgcacc accactgtat gcagttttct
    24481 gactgactgc aaatgttgca ctgtcaattt tggaatcttt cggaatctga ctaaaatttg
    24541 aatttacttt gatataagtc cggcagttct ggtgtgcagt accaaatcct gcaaggttcc
    24601 cagacttaat accatcatca aatccgacat acggataatt gttgtcacca atctgactgg
    24661 taggacttcc ctcttccaca ccgatcatgt taaaggaact cttctgaatc tctactggtg
    24721 tcggatcgat cctgaccgga tactgacgtt cttctgccga aagccaatcc ctatctggtt
    24781 ttacggtcag gattgttttt ccatcttctt cccgaagttc caatgtgatc aggaaactga
    24841 tttccccggc agcatcttcc attgatggcg tctccagaag atacttcgca tctttgattg
    24901 tcttcccttc cggatagata tagacctgat tgtcttttac ttctgcctga taccccggaa
    24961 tctgcagctc gtattcatag acttccctgt ctgttggctg ctgaagtacg atatcttcct
    25021 taatgctgga atccagtact gtatactgaa catctgcgcc ctcgtaaact tcattataaa
    25081 ggatcgcatt gtctttaata acggaatgtg tataatctcc atctaccgga atgatcccga
    25141 ttctggtctt tccattttca atcgttacac cattctcttc tgacatctgt tccggaagaa
    25201 ggatagcata atcgtttgcc ttgttctgat aaacttctgt tttctcttcc ttttcttcag
    25261 ttgccactac actgctggct tcctgtgctt cttcggatgc cggagtatct gcttcttctg
    25321 gttttacaag tgtattatca accagctcgg tatctccatc ctcattctta taagttccga
    25381 cataacctcc ataaacggtt gtatactgct tgtcccctgt cttataagtc ttggaaatgg
    25441 catcataatc aatcagttca ccttccggtt cttctggaat cggatagtaa tcctgtggtt
    25501 ctttcaactc cacttctttt tcctgcggat tttcttctgt ttcttccgct ggagttgtct
    25561 gttcttcctg cgtctctgta tcttctgatg gcgtcgtttc attttgtacg tccgcatcag
    25621 actgctgact cttatcttgt tctccagatg tggaatctgt aatccctgct tcctcttccg
    25681 tcaaagcctc tggtctggag aaatctgcat tcttctgtac atcctcaatg gcctcttttg
    25741 cctgtacaaa aataggctct gttccctgta gtgccatcag gagtgcaagt actaaggcta
    25801 tagccgactt tcgaaatctc ttcatagtct ctttctccct cttgtaatct ttttctataa
    25861 ttcctttaca aaataaccgc taccctcctt ttttctgact ttggcactac tataatctaa
    25921 aaaatcacaa aattttcata aatggtaaaa atgccccttg aggtggcaaa aatagacgaa
    25981 atcggtcgaa aaaaatagta agacaccatc gccttactat ttttcatcta atttcatata
    26041 taataatatt ttcacttgaa aggatttctc cgtatggcag atctccatct gaccatgata
    26101 tttttctact acgagtttca cattttgtaa tcctattcca tgtccttctt tcctgctttt
    26161 cgttgtcaga taaattccat tccgcacttt tggtgtatct tttatgctat tctcaatctg
    26221 gatattcatg attccttttc cataatacat ctgaaacgat aatctccttt catctgaagc
    26281 tgctgtccca cgaatagcat tatccataag atttcccact attatactta tatcaaataa
    26341 ttcttcccct atatgattcg gtatttctac agaaatctcc ggattttcaa ttactgattt
    26401 cgaatcttcc aaaatataat tcagcagatt attcatatta cggttttctg tatataaatg
    26461 atgacataag gctgttccgg ctatattctc catttgtttc agatacgata gaatctcttc
    26521 atttcctccc gtttttgcca ttgcccctat tgttgtaaga tggtgcttgt aatcatgatg
    26581 tatcttattc agcctgtcct gtgatttcat catcatctcc agttcttttg catactggct
    26641 catctgaatt ttcatctctt cctgttttgc tgtttcaata taacttttct gaacctcatc
    26701 gtgcagatag aaaataaaca cattgataaa cagtaagaat aatacaatcg tagaccgcac
    26761 acctcttctg tctggcagca atatcctgat acagactata atgctgatca ccgggacaga
    26821 aagcatagca agccaatgac ggttctttat ataataactt ctctttacac ctacttttct
    26881 ttcaataatg acttccagta aaaagaatat aaaatttccc attacaatag ttcccggtcc
    26941 catgtgatga tgcaccggat ttcttgctac cgcataccgt tgaaagaaca cgattggaat
    27001 cccatcacat aaaattccca taatatatac cataagtgta ttccagacat gatgttgcca
    27061 gtctgcctga tatgccactg ttataattaa cagagctatc agctcaaaga tcagatctcc
    27121 gtacatcgtt ccccactgca tgcttcccca gcatgaaagc agatatgcac aaaaatatgc
    27181 aaatagcttt tgtgtatcct cattttttgg atacaggatg gtattgtaac ggaacacagt
    27241 ataaatccag aataatttac ttgtcagata agtgatcaaa tggatattca taatcttttc
    27301 ttctccgttt gtaatagtct ttccctgacc ttcttgcgat ttgctttact gatacttaga
    27361 cgtgtcttgt ctttcataac cacgttgtca tattcgtaat gctccacgtg ttccatattg
    27421 atgagatagg acttgtaaat cagtaagaaa tattcttctg ttacatccgc tacatccttt
    27481 aattttcctc gatattcata ttctttttcc gatgtcacga tatgtactct ttttcccaga
    27541 ctataaaaat acaggatctt gtcataagaa atcttgtgga ttccttttgt atccgtatat
    27601 tcaaatattt tcttccgttc ttttaacagt tcccgtaatt cacctatcac ccggaaaagt
    27661 tcttcctctc ggattggctt tacaagaaaa tcaaatggat gaatacggaa taatttcata
    27721 gcatattcct gatatgttga aatataggta atccgcagtt cttttccata atttttcttt
    27781 cgtatctccg ttccgatatc aattccattc tcatgttcca gacaaatatc aaggaataac
    27841 atatcgtagg attcttcttc cagcctgtta agaagctgct ccgcagaata gtatatttct
    27901 atagatatct gacagtcatt cgctttccca taatggagca gcttctgttc taaccaatta
    27961 cataggatga tgtcatcatc acagattgca agttctagca tattttctta ccctcagtct
    28021 ttcgtaacac gacaaatggg gaaaagaatc cccttcaatt cttttcccga atggacatca
    28081 ctaattttgt gtcatttttc cgatgcaaca gcaccacttc ctttgctgtc ttattttgtc
    28141 ctctatataa taaacacttg aacaagaaaa aactgtctcc attttataat ttatttatat
    28201 tttttatgtt ttatttatat tttatccaaa tattacagcg ataactaata atgtcacccc
    28261 ctgcattatt ctttttattt ttagcgtcat ttgataaaac atcaacgttt ttgatggcaa
    28321 aaagggaacc tgcaaaacca gattcccttt aaaatacatg ctcttctcta aaaggatgat
    28381 aaatccacca tcctctgctt attttta
//
LOCUS       DACCQP010000029        28278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCQP010000029 DACCQP010000000
VERSION     DACCQP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.029"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(64..333)
                     /gene="rpsN"
                     /locus_tag="IVO87_001408"
     CDS             complement(64..333)
                     /gene="rpsN"
                     /locus_tag="IVO87_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7449609.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            523..1470
                     /locus_tag="IVO87_001409"
     CDS             523..1470
                     /locus_tag="IVO87_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7449610.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1776..4481
                     /locus_tag="IVO87_001410"
     CDS             1776..4481
                     /locus_tag="IVO87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7449611.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            5079..6902
                     /locus_tag="IVO87_001411"
     CDS             5079..6902
                     /locus_tag="IVO87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7449612.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7044..7229)
                     /locus_tag="IVO87_001412"
     CDS             complement(7044..7229)
                     /locus_tag="IVO87_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7449613.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            7440..7712
                     /locus_tag="IVO87_001413"
     CDS             7440..7712
                     /locus_tag="IVO87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449614.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            7705..7968
                     /locus_tag="IVO87_001414"
     CDS             7705..7968
                     /locus_tag="IVO87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7449615.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            7968..8234
                     /locus_tag="IVO87_001415"
     CDS             7968..8234
                     /locus_tag="IVO87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7449616.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            8435..8923
                     /locus_tag="IVO87_001416"
     CDS             8435..8923
                     /locus_tag="IVO87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7449617.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(9464..10135)
                     /locus_tag="IVO87_001417"
     CDS             complement(9464..10135)
                     /locus_tag="IVO87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449618.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(10132..11049)
                     /locus_tag="IVO87_001418"
     CDS             complement(10132..11049)
                     /locus_tag="IVO87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7449619.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(11046..11687)
                     /locus_tag="IVO87_001419"
     CDS             complement(11046..11687)
                     /locus_tag="IVO87_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449620.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(11691..12272)
                     /locus_tag="IVO87_001420"
                     /pseudo
     CDS             complement(11691..12272)
                     /locus_tag="IVO87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            12430..12834
                     /locus_tag="IVO87_001421"
     CDS             12430..12834
                     /locus_tag="IVO87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7449621.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            12851..13999
                     /gene="tnpB"
                     /locus_tag="IVO87_001422"
     CDS             12851..13999
                     /gene="tnpB"
                     /locus_tag="IVO87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7449622.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(14152..14757)
                     /locus_tag="IVO87_001423"
                     /pseudo
     CDS             complement(14152..14757)
                     /locus_tag="IVO87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            14950..15981
                     /gene="queA"
                     /locus_tag="IVO87_001424"
     CDS             14950..15981
                     /gene="queA"
                     /locus_tag="IVO87_001424"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7449623.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            16600..19239
                     /locus_tag="IVO87_001425"
     CDS             16600..19239
                     /locus_tag="IVO87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7449624.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(19407..20105)
                     /locus_tag="IVO87_001426"
     CDS             complement(19407..20105)
                     /locus_tag="IVO87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7449625.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            20323..21468
                     /gene="tgt"
                     /locus_tag="IVO87_001427"
     CDS             20323..21468
                     /gene="tgt"
                     /locus_tag="IVO87_001427"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7449626.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21565..21945
                     /gene="yajC"
                     /locus_tag="IVO87_001428"
     CDS             21565..21945
                     /gene="yajC"
                     /locus_tag="IVO87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7449627.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            22251..24848
                     /gene="adhE"
                     /locus_tag="IVO87_001429"
                     /gene_synonym="adhC"
     CDS             22251..24848
                     /gene="adhE"
                     /locus_tag="IVO87_001429"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7449628.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            25077..26183
                     /locus_tag="IVO87_001430"
     CDS             25077..26183
                     /locus_tag="IVO87_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7449629.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            26200..27224
                     /locus_tag="IVO87_001431"
                     /pseudo
     CDS             26200..27224
                     /locus_tag="IVO87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            27230..27709
                     /locus_tag="IVO87_001432"
     CDS             27230..27709
                     /locus_tag="IVO87_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7449630.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            27725..>28278
                     /locus_tag="IVO87_001433"
     CDS             27725..>28278
                     /locus_tag="IVO87_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7449631.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         9085 a         5166 c         5992 g         8035 t
ORIGIN      
        1 ttttttgatg atccaaggag tggtccatgc gccctcctcc tttacattac agccagactt
       61 ggattaccag ctggcttttt ttactcctgg aatcaaacct ttatgtgcta attcacggaa
      121 ttttatacgg gacatcccga atttacgcat gtatccgcga gggcgtccat cgattaaatc
      181 acgatttttc aatcgttctg gacgagcatc taaaggaagt ttacgcaagc cttcgtaatc
      241 tttttgcgct tttaattcct ggcgtaaagc agcatagcgt tcaatcgttt cttgctgttt
      301 ttttgctttg gcaatctttg attttttagc catcaacaaa ttcctccaat tattttttat
      361 tagcgtaaat cgtaatcttt acgcttttag ataaataatt taacacgtta cgcgaaaaaa
      421 gacaactatt ttgttttgaa tcatgcgcca aaacgaaaag acattttttt tgtcaaaata
      481 aaggcataaa cgtaaactta aagaaaaagg gagatggtga ccatgcgaag atttgcaatt
      541 aacggttacg gagaagcaca agatgttttt gaagaaatcg atgctcatcc cagagaagtc
      601 ggttctggac atgttcgtgt agaaataaaa gcattcagtg tgaatcctta tgatgttgcc
      661 ttgcgtttag gagaaatgaa agagatacga acattgaaat tcccttatgt accaggtaat
      721 gatggcgctg gtattgtgac agagattggt tcagatgtca caactgttca tgtaggtgat
      781 cgagtagctg tccatgcagt cggcgggact tacggagaag aagtcgtgtt gccatcagca
      841 aaagtagcaa aaattccaga taaaatgagt tgggaagaag cagccggaat ggtcacgcca
      901 ggaattacgg cttataatct aatcaaccat ttgacggaaa tccagccgac agatactgta
      961 atgatactag gagcatcagg tgcggtcggg tcaagtctga tccagcttct ccatgaaaag
     1021 ggaatccgaa tcttaaccag tgcatccagt aaaaatgagg aaaaggtaaa aaaattagga
     1081 gcttcagcat ttgctgctta tgataaaaca aatccaggtc tgcaatttgc tgatcaggca
     1141 gaccttgtaa tcgatgcgac gaaaggaagt atcaaaggag aaaccggcat ccagatcatg
     1201 aaaccaggcg gcaggtatgt tgcattaaat gacctcccag atctagattt gcggcaaaaa
     1261 aaggaaggct tttatgaatc gtttgttcca agaaaggaat atctagatgc agaagccttt
     1321 gctggcataa tcaaagccta ccaaaaagga gcctttcatg tttttatttc aatgaatctg
     1381 tctgcttcac ttaagcatgt catccaagca catcagctag ttgaaggaca tcctcctgct
     1441 gggaaaatca tcctgtcatt cgaaaaataa gaaatacagg aataaaaaac gaaaaaagct
     1501 gaaacatttt tgtaggggca aagacaaaaa atgttttagc ttttttgcgt atacgtataa
     1561 tagataaaaa ctttggcata aaattatctt tcccttttat ttcatgataa agtaaaggct
     1621 gtaatcctta attatttctc aatatgtaag agattacaat gagacaattg ccgtgatatg
     1681 tccaaaaatt cgactttttt gaaaaaagcc gattgaaaaa agtatgaaat acatggtaaa
     1741 gtaattcagc tgaaaaaaga agaaggggga gaactatgca gtcaatcaaa aatacgcttc
     1801 gattatattt gcttgactgg aaacggattt tcaaaaaccc gattgcaaca cttctgatca
     1861 tcgcgatcat gatcatccct tcgctatatg cgtggtttaa catcaaagca ttatgggatc
     1921 catatggaaa tactggagag ctgccaattg ctgtttacag tgcagacaag ccagcagagt
     1981 ttcaaggtaa agaagtggcc attggggagc aagtcatcga atcgcttcat aaaaataaac
     2041 aattaggatg gcaattcgtt gattcaaaag aacaattaga agacggagtc cgttccggaa
     2101 agtattacgc aggtatttac ttgccaaagg atttctcaga agatctgctg agttttacta
     2161 gtggtgatat caagaaacca aaaatcgaat atacggtaaa tgaaaaaatc aatgcgattg
     2221 caccgaaaat caccgataaa ggagcaagtt cgatccaatc gcaaatcaca aatgaattta
     2281 tcaaaacagc cagttcgact ttattgaaag tattcaatga gataggttac gacatcgata
     2341 cgaacctagt aagtatcaat aaagttaaag atatgatttt atcaacagat gaaaatttgg
     2401 acacgatcga cggctacaca aaacaagtcc tcgaattaca aagtcaacta cctgaaatca
     2461 aggaaaaact gaataaagca aatgaattcg tggattacat ccctaaagta gatgagatgg
     2521 gtgaaaaagt cgttgcattg aatgacaaaa tgcctgagtt gaaagatcaa gcaaaaatca
     2581 tccttgattt gcaagaaaaa atcccagaga tccaaaatgc cggtaagcaa ctagcagaga
     2641 tcgacggcga ttttgcttcg atccaagaga caatgaacca aggaatcgat gaagcaaaac
     2701 aaggcttgac gatcatccaa caagtacaag atatcttgcc agatgtgaaa aatttagaaa
     2761 atcaggcaag tgatctagca aaccaaacaa aatcaggtgc ctctcagctg aaagatgcga
     2821 taccaagcat cacaaacagt gtagatgtta cattgaagtc aattggacaa gtggcaacaa
     2881 cgacaggttc gttaatagat actgtaagaa cagctattga agatggtaag ctgactgatg
     2941 atgaaaagaa aaacatcagc aatgtattga acggctttat tgacggaata tctcaacaac
     3001 aaaatgcaat cgataaactg gtcgagttcc tgactaaatt gcaaacaagt gctggaaata
     3061 cgaaattaca gccaattatt gatcagctaa atcaattaaa acccttgcta agcaatctgc
     3121 aaaaccgcct atctgctttg aacacagctg ttcaaaatga agacacgaag gctattgatg
     3181 aattgctaga tcaaattgat gccgttgtta ataatatcaa tggcatcatt gctggtatca
     3241 atacaggtca aatcagttcc actgtcactt ctctactaga ccaagtcatc gatacgattg
     3301 ggactgcaca aggcgcactg agcaaagcgg accagatcga ctttgattcg cttctcagct
     3361 caacgaaagc aactgtaacg aatgcagtgg caattttaga aaaatatcaa aaagaaatgc
     3421 ctgcaattgg acaagaaatc catgatgcca atacaatgct gaatggaaat atggatacca
     3481 tcgtcaatgg catcaatgaa ggggcagacc tatacaacaa tgaattaccg atcattcagc
     3541 aaaaattagg attggccgct gattttatcc aaaatgattg gccagcacta aaaaatgaaa
     3601 tcaccggtac attgacaaca gtaaatgaaa aaatgccaga agtcgaaaca gctgtgaacg
     3661 ctgctgcgga cttgatcaac aatgactggc caaatatcaa agctggtatc caaaaagcag
     3721 cagacgcgat ccgaaaaggc gaacaagaag tcgatctagg cgaagtcatc aaattattga
     3781 aactagacgc aacaaaagaa agtgacttct ttacacaacc tgttgagcta caaaccaatg
     3841 cgctttatcc aatcgcaaat aacggttcag ctagcacccc gttttatact gcactatgtc
     3901 tgtgggtcgg cgctgttctt ctttcaagtg ttgctacgac agaatactat ttaggaaaga
     3961 aagaccgaaa caattttacg aaacgagaac aattcgttgc tcgtatgctg acatttttga
     4021 caatggcgat tgcgcaaagc ttgatcgtta cattagggaa tattttctta ctaggagtag
     4081 atgtccaaaa tccggtctac agcgtgtcat ttgctgtctt gatcgcctta gcgttcatga
     4141 tgatcgtcta tgtcttggct gcattattcg ataatgtcgg taaagggatc gctattatca
     4201 tcttagtact gtcgatctct ggcggaggcg ggaactatcc gatccaagtc tcagggaaat
     4261 tcttccaaat gatcaatccg ttcttgccat ttactcatgc ggtaaacttg ttacgggaat
     4321 ctgcaggagg aatttattgg ccaaatgcta cgaaagcaat ctggatcatg atcggtttgt
     4381 ttgttgtctt tggaatcgta ggaacggcaa tctatccgtt catcgaagaa aaaatgacga
     4441 aattcaaaga agcggcgcat gagagccata tttttcatta aaaaaagagg ttgtgaaatc
     4501 agtgttttga cctgagtatt agagaaaaaa atggaaaaat agttcctcat attttgccgt
     4561 ttttttgagc taataccgag ggtcagctga ttgaacaccg tgtaccaaga ggttgtaaaa
     4621 aagctgctct gcatcaagta ataagaacga ccaaataaaa acagcttctt atgtttttaa
     4681 tttggttggg cttattaccg cagatgcaag cttttaaaca ccgtttatca agaggttgtg
     4741 acaactgcgc tatgcttcga gtagtaagaa tgatgggtag aaagcagcct tacatctctc
     4801 ttccaaaagt taagatatca aaaaggatag aacttgattt tctaagtcaa gttctatcct
     4861 tttattatag aattcaaaga aaaagcccat aaaaaaatgt tagatttttg ctgtattaaa
     4921 gcgtttgaaa aagcgcttga tatttttctg tttttttcca attattttct taaagtttat
     4981 tgacttttaa ggtcagtgag cgtactataa catcttgttt actataaatt ttcgactata
     5041 aaacttgaag taaaattgtg tgtaaaggag tttagaaatt ggattactta aaaaggttgt
     5101 tagtaggtaa gcctttaaaa tctgctgaaa atgatgagca caaattaacc cgatttgctg
     5161 ctttggcatt attgtcgtct gatgcgttat catctattgc atatggtacg gaacaaatcg
     5221 ttgtcgtttt agtcgctttg tctgctgcag ccatctggta ttcgctaccc attgccgctt
     5281 ttgttattat tttattgatt tcattaacgc tatcatatcg tcaaatcatc catgcttatc
     5341 ctcatggcgg gggagcatat gtcgtcagca gtgagaatct tggaaagaat gctggcttga
     5401 tttccggtgg ctctttattg atcgactaca tgttgacagt agcagtatct gtctcagcag
     5461 gtgctgaagc gatcacatca gcggtgccag cactctacgg tcatcaagtc gctatttctg
     5521 tgacaatcgt tttgctactg atgatgctga atcttcgtgg gctgagggaa tctgcatcgt
     5581 tcctattatt tccggtgtac acatttatcc ttgtgatctc gttactgatc gttgttggtt
     5641 tatataatat cgtgacaggt gctgtgccat tgcaagctac tgcacttcca ggagcggctg
     5701 tgccgggtgt ttctatcgct ttgattttac gagcattttc ttctggttct tcctctttaa
     5761 ctggggtgga ggcgattagt aatgctgtac cattctttaa gaagccaaga gcaaaaaatg
     5821 ccgctgctac attgacgatg atggcattga tcttaggttt cttcttcgtg gggatcacat
     5881 ttatcaacta ttggtatggg atcgttcctg aaaaagaagt cactgttttg tctcaaattg
     5941 gtaaagctgt ctttggacat ggtattttgt attacgtcct tcaatttgct acagcgctga
     6001 ttttagcagt tgctgctaac accggttttt ctgcttttcc agttcttgca tacaatctgg
     6061 caaaagataa attcatgcct cacatgtatc aagatcgtgg ggatcgacta ggctattcca
     6121 atgggattat cacactagcg ctaggttcga tcgtattgct gtttattttc catggctcaa
     6181 cagaacgctt gattccgtta tactctatcg gcgtgttcat tccatttgct ttatctcaaa
     6241 cagggatggt cgtcaaatgg aaaaaagaag gaaaaagatg gttaagcaaa tcgattgcca
     6301 atattacggg tgcgttcatc tcatatgcaa tcattgcgat tttgtttgtc tatcggttag
     6361 gggacatttg gcctttcttc atcattatgc caatcgttat gttcattttt tataagatcc
     6421 atgatcatta taaaaaagta gcagaacagc ttcgtttaga aaatgatgca aaactacatg
     6481 attatgacgg gaataccgtt cttgtattag tcgggaatgt gacacgggta aatatcggtg
     6541 cgttgaatta cgcacgttcg atcggtgatt atgtcgttgc gatgcatgtt tcgctagacg
     6601 aagatgtcga aaaagaaaaa gaaatcgaag cagaatttaa aaagcatttc ccagatgtcc
     6661 gtttttcaat cgtccactct tcttatcgct cgattgaaaa tccaattatc cgttatgtag
     6721 atatcgtaag taaaaatgca gcgaaacaaa actatacaac tactgtgttg attcctcaat
     6781 ttgtaccaaa cagacgctgg cagaatattc tccataacca aacaagtttg cggttaagat
     6841 tgcgattgtc ttggcgtgaa aatatcgttg tcagcaccta tagctaccat ttgaagaaat
     6901 aaaccaagtt ttaaaatcaa aaacagaccc tctagctctt aattgaagct agagggtctg
     6961 ttttgtctta ttctcagcta aaggtgccct ctataagcca ttaaagatgg gaacagcgta
     7021 tgaaatgtta ttattcttcc tatttagctg taaaacattt tgattttgat gtcttgatca
     7081 taatttccga atcctttgcc aaataatgta cagcctccta tattttcagc ttcttccttt
     7141 acctcgactc ttaagtgcca tgtgtcttca taagtaggaa tctcagcctc gtcaagtgac
     7201 cattttttgt taccataact cagtgccatt tttaattggt gattcacaaa cccaacggtc
     7261 taagcaagta tcaaatcgat agtgaacatg aatgacagga gacgaaagag gcaatcattt
     7321 ttgaggcagt agaagacttg acggccaaaa tcagttttga ataaccgtta caaatctttc
     7381 gtgcgtatcc tcaggttatt caagatatca atcaagtcac actgcaacga ggaaccttcg
     7441 tgtattggtg ttgttgcata gactcacagg ctttggattc gacctttgct aaagatgata
     7501 cgttgttacc aattacttta cgtttggcta ccgctagtcg taaaggagta agaagtgagc
     7561 gtggacaaag atcctcccta aaaaggggtc tttttctcta tgtaaaaaag aggaaaagtt
     7621 tgctgaattt aagtgcttta ttgtattttt ttgacgtact taatcaagta cgctataatt
     7681 atatcagaaa aaggaggaga gacaatggct aatacaacga tgaatcctag cactgctcgt
     7741 aaaaattttt atcagttatt gaaagaggta aatgaaaatc atactgaaat cgagattatt
     7801 agtgatcgta gtggaaacaa tgctgtattg attgggctgg aagactggag agcgattcaa
     7861 gaaacccttt ttcttgagca aacaggtact ttagataaag tacgagaccg cgaaaaagac
     7921 gacagtagct ttacgaatat cgatgatatt gattgggaag ctctttgatg agtaactata
     7981 cagtcgcaat caaaaactct gcgaaagtgg atttaagaaa aatcaagcaa acgaatttaa
     8041 agaaacaatt tgaagaagtg attcagacat tgaaggaaga tccatatatg cccacgcagt
     8101 cttttgaaaa gctgagacct acacatgaag gcagatactc acgcaggctg aatagacagc
     8161 atcgagtggt ttacaaagtt gatgaggaaa ataaagttgt agaaatttat tctgcctgga
     8221 cacattatga gtgaagtgag aagagccaac ctttttagat agttaacaaa gagtctcccc
     8281 aaaaaggaga ctcttttttt gttctaaaac caaccttctt ggatttattg catgatttct
     8341 ttaatttgtt cctatatctc cacctttgaa gggtcattgt ttgagtttag gaatggaatg
     8401 ttgacgggtt tatgtaaaac aaggaggaag aagtgtgaca aggaaaatca gtaaaattgg
     8461 agcatcgttg ttagtgttgg cagtggcaac gaccatcgat tatcgccaaa gtgaggcgaa
     8521 gacaattgga aatgtcgttt atcgagaaac agccaaaagt atgttgaaaa aagcaatgga
     8581 caatcaaccg gagtcgtctt attggttccc agaggattcg ttggcatggt cttttggaaa
     8641 agatacagat acgaaataca ataccagtgt cgttccttta gcagagcggg tatccaaagc
     8701 gacattgccg aacatgaatg tcactcaaac ggaagaagtt aaagtcgtgg cgctttcggc
     8761 catgaatagc agtatcaacg gcaatgcatc acgagggatc gatatctttt acgccaatgt
     8821 cttttcttat tggcaatata tcgatcaatt ggtttacggg ggtgctcttc tggtgaagga
     8881 tcatcgtatc gccaagtcta gttgcgacgg atgcagtcca taagaatcgt gtaccggttt
     8941 taggaaccgc aaacctccca tggcgagaag ctggaatggc tagatatgtt tttagccaag
     9001 gatgaggatg gcaagttccc gattgtataa aagatgatcg aagttaccga tacggcagtc
     9061 accagttttg atgatgcagc tgaaggcatc gaacaatcga ggactgatgg aaaaggctta
     9121 aatgaaagcc acgccaaagg atgtaagaat tgatgcccaa ttcaaagaac aagcagacga
     9181 gtatttagag atcatgtggt atgaattcca tgacgagtga tgggaaaatg gattggcaaa
     9241 atgccttagc ggataagaaa aaaacttagt cggtggatgc agatatgaac ccgttggcag
     9301 tatgaagttg gagtatacgg aatggataaa tagctaagaa aagcaactct ttgcccataa
     9361 ggagggaaga atcagagtgg attattgcct taaaaatgac agaactaaaa aagcctttgc
     9421 gaccaacaaa tggtcgcaga agcttttgtt ttttacaata acatcagcgt gatgtacggc
     9481 tggctggatc tagccgtctt tccaaaatac ctaaaagcca gtccgtgatg atcgccatta
     9541 atgcagttgg caatgcacct gctaaaataa tcgatgtgcc ctctgttgca ttggttcctc
     9601 ggataatgat atctccaaga ccaccagcac cgacgaatgc accgatcgcg gttatcccga
     9661 tggcaacaac taacgcgttt cttatgcctg ccatgataac agaaacagag agtggtaact
     9721 ctaccatgaa tagtcgttgt ctagcagtca tccccatgcc tttgccgaca tcaaggatgt
     9781 ttttgtctac ttggatcatc ccggtatacg tattcttgat gatcggtaag agggaataca
     9841 aaaatacagt aacaatcacg acattgactc ccaggcctaa tccaatgatt aaaatggaga
     9901 tcatggctag agaagggatc gtctggatga tattcgccaa acgaatcacc cagtttgcta
     9961 atttacgtct cctagaaatc atgatcccaa ccggaatccc gacaactgct gcaaagagga
    10021 caccatagat agaaatcagg aagtgacgga taaattgtgc aaagatatag gagccatttt
    10081 cctggaaata atagaaaaat tgttgaaaga cattcatttt atccaattgc ttcacttccc
    10141 ttcaaaatag tggttttctt gtaagaaacg ttgtgctact actgatggtt ccactagatt
    10201 attatctgct tcgtagttca attcctgcat tttttccgta ctgatggtat gacctaactt
    10261 atttaaggct tcttttaaat gaggcgtatc agctaatatc ttgttgtcta caactggagc
    10321 agcatcatat ggtgggaaga attgacgatc atcttccaac atgactagat cataactggc
    10381 gatccgtcca tccgtagagt agccaagaac aatatccatt tttcctgctt cgactgcgtc
    10441 ataaacaagt ccaatctgca taggtaatat cgaattgaag gagaagccat aagtttcttg
    10501 gaagccgtca taaccgtctc ctttacgatt gatccaagaa gtatctacac cagctactaa
    10561 ttcatccgct acatttttca gatcgctgac tttagataga tggtattttt gtgcggtgtc
    10621 ttttcgaaca agaaagacat acgtattttc aaaaccatag gacgggaacc acgtttgacc
    10681 gtatcttttc tcaaattcat ctttcacggt agcaaacgct tttttcggat ctttttcagg
    10741 aggaaggttc aatgtagtcg ttaaatctgt tcctgtgtag cgagccgctg aaattgaagc
    10801 atctccgttc atcatcgctt gatggttgat agttgtagta gccagattgt tgatgattgc
    10861 ggtattttta tctgtataat gttcaaccat tccggcaact agacttgcta agatctgtgc
    10921 ttctgaagtg attccacctg tgatcgaaat ggtgtcttca tctgtattgc tggcaagacc
    10981 agggaaggaa caactcgcaa gtataagaag agagaaaaga accatggtca tttttagtat
    11041 acgtttcatt cttcattctc ctctctaagt gcgatcggtg tcagccggtg ttccaagaga
    11101 cctagaagcc aatcagccaa tagtgctaaa attgttacag ggatcgtacc tgccaaaatg
    11161 agatctggtt ggtaattatt cagtccacta aaaatcaaat cgcctaaacc accagcgccg
    11221 atataagagg caagtgtagc ccatgcaatc acgtaaacgg cagctaaacg aataccagcc
    11281 atgatcgttg gcatagcaat gggcaattca acggaaaaaa tggattggaa ctcagtcatc
    11341 cccatgcctt ttgccacatc ccgataattc caatcgacat ttttcatccc aatgtacgta
    11401 ttacgtaaaa tcggcaataa tgaataaata aagagtgcga taatcgcagg taatttacct
    11461 acgccaaata gcgggatcat caatgccaat aaagcaagag aagggattgt ctgcaaggca
    11521 ctggttaagc caatcacaat cgtggcagct tttggaaaac gtgtcagtaa gacaccaagc
    11581 ggtacagcaa tgatgatacc aatgatcaaa gcaagtcctg aaatataaag atgttcaaaa
    11641 ccttttgaga gaatttctga accgtgctga tgaaaaaact gctgcatagg tcagacctcc
    11701 ttagattctt caggagtagc tgactctgca acatcagtag ttgcgggatc ttctccccaa
    11761 atcacatcgt agacgatatc cactaatgat gcacgagtca ggatacctac gactttgttc
    11821 tgttcatcaa cgaccggcac gtatttcagc ccgcgtttta aaatgcgttg caaggtatca
    11881 cgaagatagg cggattcttt tacgtaaaaa acatgtttat tcaaaatgtc gccgacactg
    11941 gatacttttc ctcgtttttt attcaacgtt tctacatcga taaatccttt taatacattc
    12001 gaattatcgg taactaacaa cgtatctaca cgtttttccc tcatcagttt gattgcttct
    12061 tgtaatgatt tttccggtgt aatcgtgatt gctgtattta gcataacttc gccgactctt
    12121 gtcgcatttg gcttggcttg tagtaggcga tcttctccaa tcaactcttc tacgaaatcg
    12181 tttgcaggat gacgtaaaat attttctggt gtatcaaatt ggatcacacg gccttcactc
    12241 atgatggcga tacggcttgc tagtttaaca gaattaggca gaagtcgccc atcctctata
    12301 ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg gtaactcgaa gaccgacaac
    12361 taaggcgtat tgccttttta ttttgttcca tgttacaatc agtctataca agaaatgagt
    12421 tgatataaca tggaattaga tacaaataat cattcagtat ttcttcttta ttaccacctt
    12481 gttttagtaa cgaaatatcg tagacaagtg attgatgatg aaatatctga ttatgccaaa
    12541 actacttttg aaagaatttc agaatcgtat cacattactt tagtggagtg gaatcatgat
    12601 aaagaccacg ttcatattat gttcaaagct caacctaaaa cagaattgac aaaattcatc
    12661 aatgcctata aaagtgccag ctcaagattg ataaaacgtg actttccaag agtcaaacaa
    12721 tttctttgga aagaaatgtt ctggtctaaa agtttttgtc ttctgacaac tggtggcgca
    12781 ccaattgatg tgattaagaa gtatatccaa aatcaaggaa acaatcataa atagaaagta
    12841 ggtgaacttt atggaacaac taaaagcata caaattcaga atctatccaa cagaggaaca
    12901 agaaatcttc tttgccaaat cttttggttg tgtccgtaag gtctacaatc taatgcttga
    12961 tgaccgaaaa aaggcgtatg aagaagttaa aaatgattct tctaaaaaaa tgacttttcc
    13021 gacaccagct aaatacaaga aagaatttcc gtttttgaaa gaaattgata gccttgcttt
    13081 agccaatgcc caacttaatt tagataaagc atacaagaat ttttttcggg ataaatccgt
    13141 tggatttcca cgtttcaaaa gtaagaaaaa tcctgttcag agttatacaa cgaataatca
    13201 aaatggtaca gttgctttga ttgatagtaa attcatcaaa gttcctaaat tgaagtcatt
    13261 agtaagaatt aagcttcata gacaaccaaa agggattatc aaatctgcta caatatcacg
    13321 tcattctagc ggtaaatact atatttcttt gttgtgtaaa gaagaaatta tcgaattacc
    13381 taaaactaat tccacaattg gtattgactt aggtattatg gactttgcga ttctttctga
    13441 tggacaaaaa attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga
    13501 acaacgcaaa ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga
    13561 agctaaaaat tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa
    13621 tcaacgtact gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat
    13681 ctgtattgaa gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat
    13741 ttctgatgtt tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg
    13801 acgaaaaatc atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg
    13861 acataaagat ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac
    13921 tcatcatgat cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca
    13981 ttctatggga ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg
    14041 gtaaataaga gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa
    14101 gaagctccca cttcaagcgt taagaaccgt caggtttagc taagtggtga gtagttcact
    14161 cgcttcatcc atgtcatggg ttacaaatac gatcgttttt cccaaacgct cttgtaaatc
    14221 tttgacaagg tcttgcaagg aatcacgagt gattggatca agcgcaccga atggctcgtc
    14281 catcaagatg atatcttgat tcgctgcaag ggcacgaacg actccaatac gctgctgctg
    14341 accaccagat aattcatgtg ggtagcgatc taacatctca cggggcaatt ccacaagatc
    14401 aatcattttt tcagctgttt tattccgttc ttcttgactg actttcaaca atttttggac
    14461 aagagtgata ttttcacgga tcgtcatgtg aggcatcaat ccaatacttt ggataacata
    14521 gccgatctga cgtcgcaatt ccactggatc aatttcttgg atggattttc catcaatcaa
    14581 aattttccct tgtgttggat cagtcattcg gttgatcatc cgcatcgtcg tcgtttttcc
    14641 gcttccgctc gtaccgataa aacaaatgaa ctcacccttt tcaaatgaaa gattgacgtc
    14701 ctctacagcg acttttccac ctttataaat ttttgacaca ttttgaaatt caatcatggt
    14761 gccacctctt tttctatttt caatgaagaa ctatctatta ctagtatgcc taaaacaatg
    14821 aaaaaataca aatagggtag tttttttatt taggaaaaca aaaaatagaa gtgaaaaagt
    14881 gctgattttg ccaacagaac gaaaaacatg atagaatgat gaagcacatg atagaaagtg
    14941 agtgacgata tgttaacaac agaagatttt gattttgact tgccggaaga attaatcgca
    15001 caaacaccat taaaagaccg cgatcattct cgtttacttg ttttggatcg gaaaactgga
    15061 gagatggaag ataaacattt ccatgaaatc attgaagagt tacagcctgg tgatgcgcta
    15121 gttatgaata atacacgagt tctgcctgct cgtttatatg gagaaaaacc agatactggt
    15181 gcgcatttag aagtccttct tctaacgaat acacaaggag atacgtggga gacattgatc
    15241 aaacctgcga aacgtgccaa agttgggaca agaatctctt ttggtgatgg cagattgcaa
    15301 gcagtagtga aagaagaatt ggaacacggc ggacggatca ttgactttga gtatgaagga
    15361 atttttctag agattcttga atcattggga gaaatgccat tgcctccata tatcaaagaa
    15421 cgtctggaag acccagatcg ctatcaaact gtctatgcga aagaaaatgg ttctgctgca
    15481 gcgccaacag ctggattgca ttttacgaaa gaattacttg atgagatcga agcaaagggt
    15541 gttgaattag tctatctgac actacatgta gggttaggta cattccgacc tgtcagcgta
    15601 gataaaattg aagaacacca tatgcatagt gaattctacc gtttgacgga agaagcggct
    15661 gagcgattga accaagtacg taaaaatggc gggaaaatcg tggctgtcgg aacaacttca
    15721 attcgaacat tagaaacgat tggaacgaaa tttaatggag aaatcaaagc agacagcgga
    15781 tggactgata ttttcattac gcctggctat gaattcaaag tggtagaagc tttctcaact
    15841 aatttccatt tgccaaaatc gactttagtc atgttagtta gtgcctttgc aggaagagaa
    15901 ttgactttat ctgcttacca gcatgcgatt gaagagcgct atcgtttctt tagttttgga
    15961 gatgcgatgt tcgtgaaata aggttatgaa aagccgctag ttttgaataa caaaaaagct
    16021 ttacaaagat tttatgacaa agaaaaagcc gagctctcga taagctcggc tttcttgtac
    16081 ctcggaagga ttcaaataaa cagaaatgat tgtaactttc catcatacat gcaaagcacg
    16141 gtgttgaatt tcttcatgtt aaacaaaaat tttgttttcg ttacatcttc aacatttgaa
    16201 acatctcttg ttcctattca attttcagac gtttacgtta ttcaattgtt acgtatcgca
    16261 ttacttcttg tttcggatac ttcgtgatcc aaacatccat acgcattcac tatttatagg
    16321 tttgtactct acacgcatgt ctgggtgaaa cctgtacttc taacaacttt gtttgcttac
    16381 actgttctat cgagaaattc ttctgtctat ttgatggtat cctccttcct tacatctata
    16441 taatagcatc ctttggtagc gtttgcaaat ataagcactt taaattttac gctttcttaa
    16501 caaataatcg ataagccctt tatttgaagt aaataaaaaa aggatgttat agtaaaataa
    16561 gtaccttttt ttgtacgaag attttaaagg aggaatagga tggaagaata caagcagaca
    16621 atagaagaag taaagaaggt aacagaagca gatccagata ctgggttgtc ttcacaagaa
    16681 gtggatgaac ggcgaaaaaa gcagggattg aataaattcg atgaggcgcc gaaagaatcg
    16741 atgatcaaaa aattctttcg aagcttgtcc gattttacaa cgatcatcct tcttgtagca
    16801 gcggtgattt ccttttatac cgcatttgct acagaacatg gggatttatt tgaaggactg
    16861 ctgatcatcg ctatcgttgt gatcaactcg gtattggcga ttgtccaaga aggaaacgca
    16921 gaaaaagcac tggaatcctt gcaagatatg aataaacaaa cggcaactgt catacgagac
    16981 ggcaaagtga ccactgttga gtcagagcaa ctggtagtgg gtgatattct tgttttagag
    17041 tccggtgatg ctatttctgc cgatgcccgt ttgatcgaag catcacaatt acgtgtagaa
    17101 gaatcggcgt tgactggaga aagtgaagca gttgaaaaag atgcagcatt tattgctgaa
    17161 gaagatgagt cattaggtga ccaacttaat atggtattta aaggatgtac ggttgctgca
    17221 ggtcgtggga aagcgatcgt cactgccatt ggcatggcta cagaaatggg taagatcgcg
    17281 gatcttttga acgaaaatac tatgcaaaaa acacctttac aggtccgatt gaatcagcta
    17341 ggcaaacgaa tcagtatgat cgctttagcg gctgcggctt tagtatttgt gattggtgaa
    17401 ctgcaaggag aaccactcct tgagatgttc atgacttcga tttccttggc tgttgcagct
    17461 gtaccggaaa ccttgactgt tattgttacg ttgacacttg catacggtgt gcagaagatg
    17521 gcgaggaaac atgcgatcat ccgacagttg ccagcagttg aaacgctagg aaccgctaat
    17581 gttatctgct cagacaaaac aggaacgctg acacaaaatg aaatgcgcgt ccgccgggta
    17641 tggagcaaag aagatgaagt aacgaatatc gaagattcaa tgacgaacag cgcgatggaa
    17701 atattgaaaa tggctgcact atgtacagat gtgacggtcg agcaagaaga tgacgacctt
    17761 gttatcaaag gtaatccaac agaagtagcc atcgtaaggg ctgttgaaga gaattatcat
    17821 acaaaggcag aattagaaga aaaatatcca cgagtaaacg agcttccttt tgattctgaa
    17881 cgaaaaatga tgacaacagt tcaccaaatg ggcgaaaaat acatctcaat caccaaagga
    17941 gcattcgatg ttcttgctcc gcgctttagt tcaggcgatg tagaacaagc ggcgatcgtt
    18001 aatgatcgct ttggtaaacg ggcactgaga gtgattgctg ttggttatgc tacttatgac
    18061 gaagaaccgc aagatatttc ttctgaagca ctggaaaaag acttacgttt gattggtctg
    18121 atcggaatga tcgatccacc acgtccagaa agtaaaggag caatcaagcg agcgaaaaag
    18181 gctggcatca aaacagtcat gatcactgga gatcatgtgg tgactgccag tgccatagcg
    18241 aaagaattag gtatcttgaa tgatccgtca gaagcacttt caggatcaga actgcatcaa
    18301 ttatcagacg aagaattaga tgcgcgtgtc aaagctctct ctgtctatgc tcgggtaacg
    18361 ccagaagaca agatccggat cgtcaaatct tggcaacgta cgggtgcagt cgtagcgatg
    18421 actggggatg gtgtgaatga tgcaccagcg ttgaaagcaa gtaacgttgg gtgtgccatg
    18481 gggatcacag gaacagatgt ggcgcaaagt gccgcagata tgattttgac ggatgacaac
    18541 tttgcgacga tcgtagacgc tgtcagtcaa ggacgttcgg tttatcagaa tattcgtaaa
    18601 gctatcaact ttttgctaag ctgtaatatt tcggagatct ttattgtttt gattgcgatg
    18661 ctgttaggat ggggagcacc atttacagct gtgcaactac tgtttgtcaa tgtagttgct
    18721 gatggacttc caggatttgc tttaggaaga gagccagcgg aacacggcat catggatcag
    18781 ccacccattc cgaaaaatga agggatcttt gctcgaggtt tattgcaaaa aattgccata
    18841 aatgcagggg tcttcaccat cgtaaccttg tttggttatt atttaggtag ttatgtcgat
    18901 actatcagcc catgggtaga cgcaagtcag catgtcggtc aaactgttgc attcttgatc
    18961 ttagcttatt catcgattct gcatgtgttc aatgtacgca gcagccaatc gatcttcaaa
    19021 gtcaatttag cgaccaataa agctttggta gaaatggcgt tactggcatt agctattaca
    19081 acggctgttg ctttgctgcc atttacgcaa gaactattcg gcctagtaca catcagtttg
    19141 aatcactggt tcttggtagg gattttatct atcgtaccaa tcgcggtgaa tgaactgatc
    19201 aagttccatc gattaccgga agcagaagaa gaggaatgat taactgtttc taagttgatt
    19261 ttctttttac tggaactata caaaaaggag gtgtgacatg aatgttgtca cacctccttt
    19321 ttgctaatgg gctgaatgtt tcttttactg cctctcgata aacggtgttc tcctgccacc
    19381 ccctcggtca caagtcacac tctcttttaa atatcttttg gcaattgttc ataaaatgct
    19441 gcttttgttg cagaaatgta tgggctgagc catagataac caataccagc agttgctaat
    19501 gctaagatat gccagccgat aaatgaaaga tctaaccaga aaagttgtcc tttataacca
    19561 tccatcaatt ttcgacttgc agtgatcgta tccagtactt taggtgtttg tcctgtttct
    19621 tggacgatat cgtagtaaat aaaatagctt tgagaataag aatagctttt aatgattcca
    19681 ggaataacta atagcaaacc ccataatgtt gtaaagatcg agatcagcac agctaagctg
    19741 atcactccgc cgataaacaa acctttgaat ccacgaaatg catctttgaa tggttcgatc
    19801 tgatcttttt gtccacgtaa aatatcaaga taagtccaag aaataccgct catgaacaaa
    19861 gcgctaatga tgctggaaat aattccgcca ccgcctcctg aactaccgcc tcctgaagtt
    19921 gtcgcttctg agacgctcgc tgatccatca tatatggtgg tggcaaacaa gataatagga
    19981 atcataataa gaagaatagt agcaatacca atcaatgtag ggattagatt tagtaaaaca
    20041 gcttgtcccc aacgtccttt tagactctct tttgcttggc gttttaattc tgcagaagat
    20101 tgcatgattt ttccctcctt ttctttaata acaccaatat accatgtatc aaaaaatatt
    20161 tctgtcataa tgtttttctt ttttttgtta ttcataaaaa aaggataaaa aaaagaatat
    20221 taaataaagt agaaggaagc cgagggaact tgcaaaaagc acttgaaacg attatgatgg
    20281 tgggagttgt caagatattt tatatagaaa gtagggaaat gaatgacgca acctgccatt
    20341 cgctatcgtt taatcaagaa agaaaaacat actggcgcgc gtttagggga attgatcacg
    20401 ccacacggaa ctttccccac accaatgttc atgccagtag gaacattagc tacagtaaaa
    20461 acaatgtccc cagaagagtt gaaagaaatg ggtgctggcg ttattttaag taatacatat
    20521 catttatggc tacgccctgg tgaagactta gtagaagaag ctggcggact gcataaattt
    20581 atgaactggg atcaaccgat tttaacagat tcaggcggct ttcaagtttt ttcattaagt
    20641 gatatgcgga atatcgaaga agaaggcgtg catttcaaaa atcacttgaa cggttcgcgt
    20701 atgtttttat caccagaaaa agcaatcaac atccaaaata aattgggctc tgatatcatg
    20761 atgagttttg acgaatgtcc accattcgat gaaagttacg actacgtgaa aaaatcagta
    20821 gaacgtacgt ctcgatgggc agaacgagga ttgaaagctc atgctaatcc agaccgtcaa
    20881 ggattatttg gaatcatcca aggagcaggg tttgaagatc ttcgtcgtca aagcgcgaaa
    20941 gatctagtcg ggatggattt ccctggttac tcaatcggcg ggttatctgt cggggaacct
    21001 aaatcagaaa tgaatcgtgt attagaattc actacaccgt tgatccccga aaacaaacca
    21061 cgttacttga tgggtgtcgg tgctgctgat tcattgatcg atggtgttat ccgtggagta
    21121 gacatgtttg actgtgtctt gcctactcga atcgctcgaa atggcacatg tatgacttct
    21181 caaggccgtc tagtagtgaa aaatgcgaaa tatgcccatg atttccgtcc aattgatgag
    21241 aaatgtgatt gttatacatg taaaaactat acacgtgcct atatccgcca tttgatcaaa
    21301 tgtgatgaaa catttgggat tcgtttgact tcttaccaca atttatactt cttgttgaat
    21361 ctaatgaaac aagtacgtca agcaatcatg gatgataatt tgcttgaatt tagagaagcc
    21421 ttttttgaag aatacgggtt caataaagaa aacgcaaaaa gcttctaaag gaaaatcttt
    21481 taaaaattaa gtaaaaaact agtgttatca gcatttttct gctaaagtgt tttttgtaga
    21541 tacagaatta gtgaggtgaa aaaaatggga acattaccaa tgcttgtgat gtttatcgcg
    21601 ctattagcaa tgtggttttt tatgtcacgt tcgcaaaaga aacaacagca agaacgtcaa
    21661 aacttgttag atagcatgaa accaggtgat tcagtcgtaa cgatcggcgg attacatgga
    21721 gttgtttcag aaatcgacaa tgacaaacgg acagttgttt tagactgcga agggatcttc
    21781 cttgagtacg atcgtgcagc aatcaaaacg gtcaaaccag ggactgtcgt aacaaatgat
    21841 gcaacgacta ctgttgaaac aacagaagaa ccagctgaga cagttgaagt aaaaactgaa
    21901 acaacagaaa cgctggaaga gacagaagag aaggaagaaa aataagtatc aaagcttatt
    21961 taaagacgag gtagggcaga aagatgtcct acctcatttt ttattcaaaa aagaaagaga
    22021 taagagcatg ggatatattg ctcaatattg aataatcggg tgtgtcatat aaataataat
    22081 aaataacttt atatattttt ctatacaaac acctgtaaat aaagcaattt tatatttaaa
    22141 aataactgtg aaaaaaaatc acaaaaaagt gtttacatag aaaaataaat attgtatgat
    22201 atacatgtga attatttaac aaataaccat gacccattag gaggataatg atggctaaag
    22261 taatagaaaa agaaaaaacg aagacaattg acgtacaagc aatgattgat gatttggcag
    22321 aaaaagcaaa tgcagcattg aaagaaatgg aaagctttga tcaagaaaaa gtagaccata
    22381 tcgtgcatga aatggcaatg gcagctttgg atcaacatat gccattagcg aagatggctg
    22441 tagaggaaac aggccgcggt gtttacgaag ataaagcaat caaaaatatg tatgcgtccg
    22501 aatatatttg gcataatatc aaacatgata aaacagttgg tgtgatcaat gaagatgtcc
    22561 aaaaagggtt gattgaaatt gctgaaccag tcggtgtggt ttgcggagta actccaacaa
    22621 cgaatccgac ttccacaacg attttcaaat caatgatcgc gttgaaaaca agaaatccaa
    22681 tcgtttttgc tttccatcca agtgcccaaa aatcttctgc agaagcagca cgagtggtac
    22741 gtgatgctgc tatcgctgcc ggagctcctg aaaattgtat ccaatggatc gaacatcctt
    22801 cgatcgaagc gacttctatg ctaatgaacc atccaggtat cgcgatcgtc ttagcgacag
    22861 gtggagctgg aatggtcaag tctgcttact ctacaggaaa accagcttta ggtgtaggtc
    22921 caggtaacgt accagcttat attgaaaaaa cagcaaaaat taaacgtgca gtgaacgatt
    22981 tgatcgtttc gaaaacattt gataatggaa tgatttgtgc ttctgaacaa gctgtgatcg
    23041 tggataaaga aatctatgca gcagtaaaag cagaattcca agcacaccaa gtttatatcg
    23101 taaaaccaga tgaattgcaa aaattagaag atgcagtgat gaacgaagga aaatatgcgg
    23161 tcaatccatc aatcgttgga cattctgcga tggaaatcgc aaaattggca ggaatcagtg
    23221 taccaaaagg aacgaaaatg ctgattgctg aattggaagg cgtaggccca gattatccgc
    23281 tatcaagaga aaaactttct ccagttcttg ccatgatcaa agctaacaac acggaccatg
    23341 cgtttgattt gtgtgaaggt atgttgaatc ttggaggatt aggacataca gcagttatcc
    23401 attcagaaaa cgaagagttg catgtcaaat tcggtcttcg aatgaaagct tgtcgtattt
    23461 tagtgaatac accatctgca gaaggcggca tcggtgatat ctataacgaa atgattccgt
    23521 cattgacttt gggatgcggt tcttatggta agaactctgt ttctcgtaac gtctctgctg
    23581 taaacttaat caatgtcaaa actgtagcga aacggagaaa taacatgcaa tggttcaaat
    23641 taccgccaaa aatcttcttt gaaaagaact ctctattgta cttagagaaa atggaaaacg
    23701 ttgaacgtgt catgatcgtt tgtgaccctg gtatggtcca attcggttac tgcgatacag
    23761 ttcgtgaagt attggcacga cgtaaaaatg atgtgaagat cgaagtcttc tcagatgttg
    23821 aaccgaaccc atcgacaaat accgtatacg caggaacgaa actgatggca gacttcaagc
    23881 cagatacagt aatcgctctt ggaggcggtt ctgcaatgga cgcagctaag ggaatgtgga
    23941 tgttctatga acacccagac acatctttct ttggtgcaaa acaaaaattc ttggatatcc
    24001 gtaaacggac atataagatc gacaaaccag aaaaaacaca attcgtatgt atcccgacaa
    24061 catcagggac tggttctgaa gtaacgccat ttgcggtcat cactgacagt gaaacacatg
    24121 tgaaatatcc gttagcagat tatgcattga cgccagatgt cgcaatcgtc gatcctcaat
    24181 tcgtcatgtc agttccagca tctgtcacag cagataccgg gatggatgtg ttaacacatg
    24241 caatcgaatc ctatgtttca gtcatggctt cagattatac acgtggattg agcctacaag
    24301 caatcaagtt agtctttgac tatttagaaa aatcagtaaa aacacctgat atggaatcac
    24361 gtgaaaaaat gcacaacgct tctacaatgg ctggtatggc gtttgctaac gcattcctag
    24421 gtatttgtca ctcagtcgca cataaaatcg gcggggaata cgggatccct catggacgga
    24481 caaatgcgat tcttttaccg catattatcc gctataatgc aaaagatcca caaaaacatg
    24541 cgatgttccc taaatatgac tatttccgtg cagatacaga ttacgcagat atcgcaaaat
    24601 tcttaggtct gaaaggcgaa acaacagagg aattagtcga agctttagca acggctgttt
    24661 atgaattagg aaaatctgtc ggcatcaaca tgagtctaaa agctcaagga gtcacacaag
    24721 aaacactaga tacaaccgta gaccgtatgg ctgaattagc ttacgaagac caatgtacga
    24781 cagctaatcc gaaagaacca ttgatctctg aattgaaaca gatcatcatt gatgcttaca
    24841 acggataagc aaaagaaagc gtagaaaaaa gataagaaaa tagatgaaaa agaacttgga
    24901 aaacgttgtt tctccaagtt ctttttttaa tttccaagta actgaaagtt acttttgtta
    24961 tttaacattt aggtcaattt tagacagttt ttgttcaaaa agcgatattt atagtggacg
    25021 gtattactgc tatattaagg atataaaatg aaagcgttta aaaaggtggc gtaaagatga
    25081 ttattaaaga agagattttt gtacctaaag aattagtgaa agaaaaaatt gacctgctcg
    25141 ttgaaaacct gacaacgatc aaagacgata atggtgaatt tttgttggac tttgatggac
    25201 tgaaagtaga tgacaaaagc tggacagtgt ggaattggcc acaaggtgtc ggtctatatg
    25261 gtatttataa aaattaccag atgacaaaaa atcctcgcgc ttatcaagtg gtcaatgaat
    25321 ggtttgagga tcgtatggaa gaaggcgctc cgcccaaaaa cgtcaataca atggcaccgc
    25381 tattgactat ggcttacttg tatgaagaca cgaaagattc acgctatttg ccttatttag
    25441 aacaatgggc agaatgggtc atgaatgaca tgcctcggac aaatgaagac gggttacagc
    25501 atgccacata tggaccggaa aataagaatc agttgtggga cgatacattg atgatgacag
    25561 tgcttccttt ggcaaaaatc gggaaactgt tgaatcgccc agaatattta gaagaagctc
    25621 gttaccagtt tatgatccat atcaaatact tgatggataa acgaacagga ttgtggtatc
    25681 acggctggac gtttgaagga aatcataact atgccgaagc attttgggct cgtggaaact
    25741 gctggttgac gattgctatt ccagagatca ttgaaatctt agaattatcg aaagaagatg
    25801 cattgcgcaa attattgatc gaaacacttg aagcacaagt gcgtgcatta aaaacttatc
    25861 agtctgaaag cggtttatgg catacgttgt tggatgatcc ttcttcttac gtagaatctt
    25921 ctgcaactgc aggatttgct tacggtattt tgaaagcggt ccataaacgt tatttaccac
    25981 aagaatataa agaagtcgct tatcgtgcta tccaaggatt gcttgaacaa atcgatgaaa
    26041 aaggagaagt tcaaaacgtt tcaatcggga caggaatggg ggacagttta gatttttatc
    26101 ggaatatcgg aatcacagca atgccttatg gacagtcgtt aacggtgctt tgtttgacag
    26161 aattgctcgt ctcttattgt taaaagaaag gaaacccata tgaaaaaaac atcactgctg
    26221 tttgcagcaa gttcgcttag cctaggtctg ctaggtctaa gcggctgcgg aaataattct
    26281 agtgtggcaa ctgtcgcaga tgcagacgta aatgagatag ttaccttgcg ctttgcttat
    26341 gcaagcaaca gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa
    26401 cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac
    26461 tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg
    26521 aaagtttctc taaagattat tcgatttttt ccgttcctta tctattcaat gatgaagaac
    26581 atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa
    26641 atttaggatt taccggattg acctattatg attctggtca gcgcagcttt tatatggtag
    26701 acggtccgat ccatacgccg gaagatttga agggaaagaa gatccgtgtg atgcaaagcg
    26761 aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg
    26821 aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct
    26881 tgtatacagc tggacatggc ggggtagcta aatactattc ctatgatgag catacacgtg
    26941 tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat gaacaggaaa
    27001 aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag
    27061 caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag
    27121 ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta
    27181 cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtaa tgaaaatgat
    27241 tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt
    27301 tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct
    27361 tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa
    27421 agcacgttta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt
    27481 agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg
    27541 atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg
    27601 atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa
    27661 tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa
    27721 taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt
    27781 agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat
    27841 accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt
    27901 ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat
    27961 agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc
    28021 tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc
    28081 ttctaccgcg attggtggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa
    28141 gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac
    28201 tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg
    28261 aggttatctc gtaggcgc
//
LOCUS       DACCQP010000030        27651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCQP010000030 DACCQP010000000
VERSION     DACCQP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.030"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(177..1568)
                     /locus_tag="IVO87_001434"
     CDS             complement(177..1568)
                     /locus_tag="IVO87_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7449632.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1781..2167)
                     /locus_tag="IVO87_001435"
     CDS             complement(1781..2167)
                     /locus_tag="IVO87_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7449633.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2223..2846)
                     /locus_tag="IVO87_001436"
     CDS             complement(2223..2846)
                     /locus_tag="IVO87_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7449634.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2910..3578)
                     /locus_tag="IVO87_001437"
     CDS             complement(2910..3578)
                     /locus_tag="IVO87_001437"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7449635.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3590..4030)
                     /locus_tag="IVO87_001438"
     CDS             complement(3590..4030)
                     /locus_tag="IVO87_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449636.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4089..4760)
                     /locus_tag="IVO87_001439"
                     /pseudo
     CDS             complement(4089..4760)
                     /locus_tag="IVO87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4945..5343)
                     /locus_tag="IVO87_001440"
     CDS             complement(4945..5343)
                     /locus_tag="IVO87_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7449637.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5340..6113)
                     /locus_tag="IVO87_001441"
     CDS             complement(5340..6113)
                     /locus_tag="IVO87_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7449638.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVTTGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6110..6793)
                     /locus_tag="IVO87_001442"
     CDS             complement(6110..6793)
                     /locus_tag="IVO87_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7449639.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6960..7847)
                     /locus_tag="IVO87_001443"
     CDS             complement(6960..7847)
                     /locus_tag="IVO87_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7449640.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7844..8713)
                     /locus_tag="IVO87_001444"
     CDS             complement(7844..8713)
                     /locus_tag="IVO87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449641.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8850..10136)
                     /locus_tag="IVO87_001445"
     CDS             complement(8850..10136)
                     /locus_tag="IVO87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7449642.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10409..10987
                     /locus_tag="IVO87_001446"
     CDS             10409..10987
                     /locus_tag="IVO87_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7449643.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10987..11916
                     /locus_tag="IVO87_001447"
     CDS             10987..11916
                     /locus_tag="IVO87_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449644.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11913..13649
                     /locus_tag="IVO87_001448"
     CDS             11913..13649
                     /locus_tag="IVO87_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7449645.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13712..14548)
                     /gene="pheA"
                     /locus_tag="IVO87_001449"
     CDS             complement(13712..14548)
                     /gene="pheA"
                     /locus_tag="IVO87_001449"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7449646.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14545..15054)
                     /locus_tag="IVO87_001450"
     CDS             complement(14545..15054)
                     /locus_tag="IVO87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7449647.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15047..16342)
                     /gene="aroA"
                     /locus_tag="IVO87_001451"
     CDS             complement(15047..16342)
                     /gene="aroA"
                     /locus_tag="IVO87_001451"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7449648.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16355..17446)
                     /locus_tag="IVO87_001452"
     CDS             complement(16355..17446)
                     /locus_tag="IVO87_001452"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7449649.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17463..18629)
                     /gene="aroC"
                     /locus_tag="IVO87_001453"
     CDS             complement(17463..18629)
                     /gene="aroC"
                     /locus_tag="IVO87_001453"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7449650.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18631..19695)
                     /gene="aroB"
                     /locus_tag="IVO87_001454"
     CDS             complement(18631..19695)
                     /gene="aroB"
                     /locus_tag="IVO87_001454"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7449651.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19728..20750)
                     /gene="aroF"
                     /locus_tag="IVO87_001455"
     CDS             complement(19728..20750)
                     /gene="aroF"
                     /locus_tag="IVO87_001455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7449652.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21409..22383)
                     /locus_tag="IVO87_001456"
     CDS             complement(21409..22383)
                     /locus_tag="IVO87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7449653.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22364..24247)
                     /locus_tag="IVO87_001457"
     CDS             complement(22364..24247)
                     /locus_tag="IVO87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7449654.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24265..26490)
                     /locus_tag="IVO87_001458"
     CDS             complement(24265..26490)
                     /locus_tag="IVO87_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7449655.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26605..27639
                     /locus_tag="IVO87_001459"
     CDS             26605..27639
                     /locus_tag="IVO87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7449656.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         7941 a         5996 c         4911 g         8803 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agctttttca caacctctta ttcacggtgt
       61 ttcagaagta gcttctcgga aataagccga aatttcacaa aaatttgaga aactattttt
      121 aggaaacttc ttcttatttc ttaaagctga ccacttctat cacaacctct ttttgactat
      181 gcattcacat cgatcacttt agaatcatca ttgatgacat cgcgatctaa atctttgatg
      241 cgtgcacttg tcacgatacc agctaccatg ctaccgctga cgtttactgc tgtacgtgcc
      301 atatcgatta atggttcaac agagataact aacccaacga ttgctactgg caagttcatt
      361 gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac cgactccagc tacaccaaaa
      421 gaactgattg ttacaattgc cacaagcatc aaaataaatt ccaaactaaa gacattcact
      481 ccagctgctg gtgcgacgat cgttgccaac atagctggat aaacgccagc acagccgttt
      541 tgtccaatag acaaaccaaa acttgctgag aaattagctg ttgcatcatc tactcctaaa
      601 gcttttgtct gagtctcgat attcaatggc aaagctccag cacttgaacg tgaagtaaac
      661 gcaaagctca ataccggaaa ggcttttttg aaatagttga ttggattgac ttttgcacca
      721 atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg ctacgtaaga agcaacgaca
      781 aatttaccta aattgacgat agctgcaaaa tcacttgttg ccaaaacgtt tgtcatcaaa
      841 gcaaatactc cataaggtgt caaacgcaaa acaagcgtca cgatccgcat aatgatttta
      901 tacagactgt cgactaaatc tgaaaagaat gctgcttctt ttggcgcttt tcttttcacg
      961 cctagataag cgattccgac aaatgccgcg aaaataacga ctccaatcgt acttgttgca
     1021 cgactacctg ctaaatcagc aaaaacgttt ttagggataa aggataaaat ctgctgtgga
     1081 atcgataagt tttcaactgt ttcctgacgt gttgccagtt cagagattcg tgctgtttct
     1141 gcagctcctt gtgtgaattt agctccttgg agtccgaaca gcattgttga accaattccg
     1201 atcaatgctg caatagctgt tgttccaagt aaagaagtca atacagtcgc actaattttc
     1261 cctagctttt cagaaacttt catttttgtg aatgcaccaa tgatcgaaat aaacactaaa
     1321 ggcataacta gcatttgcaa gaatgataca tatccttgac ctacaatccc gatccattcc
     1381 attgattgag taacgacttt gtcgtctaca ccaaagatca attgaagcag accgccaaag
     1441 acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa attttgtatg gcgcttttgc
     1501 atacgataga acacatataa taatgccgca aaaactagaa ccactaaaac tgtgatgagt
     1561 gttgtcattt ttctcctcct catattatta atgacaagtc catcgaagga agtaaaggtt
     1621 cccgagaatg attcttcaac catcctcctg aaaaaatttt tttcagtgac ttttatattt
     1681 tagacgattt ttatcgaaat gacattcttt ttgcttacaa ttagtaataa aaaaacatac
     1741 ctgtgatagc tttaatctat cgcaggtatg tttttactgt ctattcttgt ttttctgatt
     1801 gaaaattcaa ttttacccca ggctgatcta aggcgatctc tgtcacctca taagacattc
     1861 ggcgtacgat atcgaataac ggtgctacta gctcgtccac ttcttcttgt gtgacatcat
     1921 tttgaccttc aattttccga ttgaggatat gattgatctg actcacactt ccagataaaa
     1981 catatgtatc aaacactaaa cggaactcta agcgtgctcc tacgatgcta tgttctttcg
     2041 gatagttttc gatcgtttct tttaaaggcg agaagcctac tttcaaatct gtttctactt
     2101 cttggttcgg caatctcata tcataatgaa atgcttctac aacttctttt tgtcttctga
     2161 tttccatata attttatcac tcctttatga aaattataac atatctcctt tgaaagggga
     2221 tactactttt taatgatgct ctgcaaggta aggatttcat tcagatttcc taccacataa
     2281 tctgccggga tctcacccag aaaatgatcg atgtcgtaaa gacaagtagc gactcctgca
     2341 tttttccctg cttcgatatc taaacgacga tcgccgatca tcatcgtgtc tgttctttct
     2401 aaatgaaaag tatcgatcaa ataatttaaa gatgctggat caggtttacg aggaaagtcc
     2461 tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc gatgttcttt taacaggccc
     2521 catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac gaccgccgtt gtcttttagc
     2581 atttccagta cttgtttcgt ttctggaaat ggacgggaaa cttccgtact ttctttttca
     2641 tatcggtgaa ataattcatt aaactctctg gcattcaagt ttgattcatt gatcaaataa
     2701 ctagtagaat actttttcat tttgaagtag atttcttttt tgtccatact gataccaaaa
     2761 tcttttagtg cttgccatgc accatctacc attgcgggat acgtatcaaa caatgtacca
     2821 tcaaagtccc atatataatt tttcatgttc cttttgttcc attcctttgc ttcttttttc
     2881 tatctctcac ttttagataa aggtgttctt taaagtattt ttttgtccag aattgtcaaa
     2941 tcatctgcta atgtatactg gattggttgt gcatttggga tatcgatcgt atccatttga
     3001 tcttcaggaa catcttctaa atattttgtc agtgctcgga tgctattacc atgacccaca
     3061 actagtaaat cttgccgatc ttttagtttt ggtgctagat gatcttgcca taagggaatg
     3121 atccgattca ctgtcatttc cagactttct ccgtatggga tcaaatgcgg atccaagtga
     3181 tcatatctgc ggtcaaatct attttctgcg gttaacggcg gaacttccgt atatcctctg
     3241 cgccatgttt ttacttgatc ttttccgtat atttctttca tcttatcttt gtttttaccc
     3301 acaagatccc cataatgccg ttcatttaga cgccatgttt ttattatagg aagccataac
     3361 tgacccattt cttctagtgc gacttgtccg gtaagaatcg cacgtttcaa aacagaggta
     3421 taaacggcat cgaattgaag tccggcttca agcatttttt gaccggcaac tgctgcttgt
     3481 tcttttcctt tttctgttaa ggcgacatct agccatcctg tccagtaatt ttcaaaattt
     3541 gcttcgcttt cgccatgacg taataatact aatttcattc ttttctccct taaacattcg
     3601 tttcgaattt tataccacat gttttgcatg tcacacgtat tcgtcctttt cctttaggtg
     3661 cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt ataacttttt cgatccattt
     3721 tgacttgctt tttgtttttt atctgatgat atttttcttt gactctatca agatatttca
     3781 aataacgttg attctcattt agtctggggt agattttttt tgataggaaa cgaaatatca
     3841 tccatccgaa aaagacgatg aacaacaatt gtccgattct atagggcaac caacgactta
     3901 gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt caactgatca aatttcgcat
     3961 atcttccctg cataagtttg tgatagttct gctgaaatcg tattagcctt tctaaccatg
     4021 tttgcatcat attatctcct ccatcatcta atatttatta tgacaaaaaa atccaaaaag
     4081 aaaaagcctt aattggcctt ttccattgca acttggtgaa ggacattccg atttctggta
     4141 aagtcaatca caatcacttt ctttttatct tctctgttga ctactcgaaa tgtgtccgtc
     4201 aatggaagaa acagcttctt agatggtagc tgttgcatac gtataaaacg atggatcatc
     4261 gtaatcaatt gaggaaattg aatagacgtg tgaagcagac ctggatattt tttcaacagc
     4321 tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt ttaaaaagct ttttagaatc
     4381 ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat ctatttgata acgcatttgt
     4441 ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt ttgtatcgat cattaaacct
     4501 tctggaaaca gttcaaccac tgctcgatcc caagattcaa gagaaagctc gatataatct
     4561 tttactggta attccaattc tacctaaaaa aagtgcaaaa cagccgacag cccttctgct
     4621 tgatagattt ctcctaatgt tagcccctct agatggaact ctggtgaaaa atgcagcaat
     4681 gttttatttt cctgcaattg aacatataga aatgtagatc gctcaatcag agtatctttt
     4741 aaaacaacta aaaaattcat ttttctcccc cttttttcat catttttaaa atagcatatt
     4801 ttgaaaagaa agtctgatgc ttagagtaga atttcataaa aatttattat ttcataagta
     4861 ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt cttgtttgtg
     4921 tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt tattttcatc
     4981 taaaagttct tgcattccga taatttctcg atattgatga atcttagtga taccaatgac
     5041 tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt tgatgccttg
     5101 accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca acacaatatc
     5161 tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac cagaacctct
     5221 tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga taacatcttc
     5281 gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta caagatcttt
     5341 catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt ttcatctttt
     5401 cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc aatcattgca
     5461 accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc gattgccacg
     5521 ctcattgata aaataaatgt cgaacacatc gggcccgttg ttactccacc gttatcaaaa
     5581 gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag cccgtatcct
     5641 gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga caatcctact
     5701 gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat tctcccctcg
     5761 ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa ccctactaag
     5821 aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc cgcaatagca
     5881 ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag gaaaaggatc
     5941 aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt tttgcgcata
     6001 cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac tgccaaaatg
     6061 acttccatgg taacgatttc gattccatct atcacttgct gccagaaact cattggaaga
     6121 tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg accattccaa
     6181 aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg gcaagggcca
     6241 agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa gcaatcggca
     6301 taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct atagccatca
     6361 attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga aatactccag
     6421 tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca gatatatcat
     6481 atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg atcccgatag
     6541 caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat ttccctacta
     6601 gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc attccgatca
     6661 tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa
     6721 caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc acttctttaa
     6781 aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa tgaaagagtt
     6841 ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat aacacacgtc
     6901 ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat tgtgtttttt
     6961 tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa aaatacgatg
     7021 gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc tggtgtatag
     7081 gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga ttgcaaagct
     7141 actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc agccgcggca
     7201 taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc ttttagaccg
     7261 tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg tcgaaagaag
     7321 aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact gttgattccc
     7381 aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa tgccgcaaaa
     7441 ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt actttgataa
     7501 atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt ggtcaccaaa
     7561 gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga aaacaaattt
     7621 tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat atccacgggt
     7681 gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa tgataggatg
     7741 cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa tgcttgtttt
     7801 ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga ctttctcctc
     7861 ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca aaggaaactg
     7921 ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg tattgagata
     7981 gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca tcaaaaagct
     8041 gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt aaatttggga
     8101 tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct gcttcataaa
     8161 tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat ccagtccaac
     8221 gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc aaccagccaa
     8281 tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca gattgagaaa
     8341 atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga agaaagatag
     8401 cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg gaaataaaaa
     8461 gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta ttaaaaaaag
     8521 cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg ttaaatgtca
     8581 tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc ggaataaaaa
     8641 ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt ttgaataaat
     8701 ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg aaaagacaag
     8761 aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt ctgatgaaaa
     8821 agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc tacttgtttt
     8881 tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg aagagcttca
     8941 gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg tatttcagat
     9001 gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga agcttcttga
     9061 taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc tttcgcaaga
     9121 gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt cgctgaatct
     9181 tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga agcttgcgct
     9241 ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc agattggttt
     9301 tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc tactgctcct
     9361 gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag atcttctttc
     9421 aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg gatcgtgact
     9481 gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat ccttactcga
     9541 ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt gacatctcgc
     9601 gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt ataacctgct
     9661 tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa aggaacaccg
     9721 tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg atcttccttt
     9781 acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc ttgtatccta
     9841 taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc taaagcggtg
     9901 ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc tgtttcttcg
     9961 ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc atcccacgcc
    10021 cagacagtga ctttttcttc tttgtctgct gtactactag cacttccgca tcctgttaag
    10081 aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat gttcattttt
    10141 atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat accaaaattt
    10201 gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata ataaaaaaat
    10261 acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc gaaatggtga
    10321 ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat ttttaaatta
    10381 caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta tcgatgatga
    10441 acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg ggtttcaatt
    10501 aatagggact ttttccagtc cgctgaaagc gcttgatttt tctttggtac atgcaatcga
    10561 tatcgtggtc acagatgtaa tgatgcctga attaaatgga atcgaattat cgcggctatt
    10621 aaaacagcaa caacctgaaa cacagatcct tatcctctct agctatgatg aatttgatat
    10681 ggttaaagat tcttttaaag aaggcgtcgc tgattatctt ttaaagcctc gattgagtcc
    10741 tgattttttt ctaaattcct taactgccgt ttcacaaaaa atcaaaaaaa agcccaacaa
    10801 aagcccaatc accaatcgtt atgagcagat ctgcgagaat ctttcacagc agattgctgg
    10861 acaaactcgt ccattgcttg accctcttct ttttgaacac accttgtttt tctgttatat
    10921 accgaaaaac ggccattcag ttcaaaacaa ggctctaaac aaaaacagtc gatcacccat
    10981 tcattgatgg atagcgacga ttcgtttact atttatcctg tttctacctt ttcggaagaa
    11041 tccggctatg taatcaatac tgattccaaa gatacactta ggaagtttgt tcaaaagttt
    11101 cgtcaagaag tttttccagg tgattttttt gttgtttctg attcttttgc aatccaagat
    11161 atcaatgaaa tattccaaga gatcagaaat tgcaccaaag gacaacgttt ctatcataag
    11221 ggaaagaacc ttgcttatca gcatgaactt cttccgttat taaccaacca cgagcagcaa
    11281 gcaagtagtt atttaacaaa aatcgttcat aaagactacg ctttagctgt caaagaactt
    11341 gagaatttct tcacgacgat attggaacag cctattgatc cagcttattt gaaacatgag
    11401 gcaatcaatc gtttctatgc attactcaac gctttggctg acgagcatgt tgggaatgaa
    11461 gaactttctt tgctcaagat cgcccttcct tctttattag ccgatgctga gtatttcgaa
    11521 gatttttcta ttcttgtcaa agaaacactt gagaaactca cccacttttc tgcaaatgca
    11581 aaaaaagaca atttcgatat cttgactttg atctacgagt tcgtccagga aaattatgag
    11641 aaagaaatca atttacaact tttatccgaa aaatatcatt tttcatatag ctatctctca
    11701 gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat atctaaaaaa agtccgtatc
    11761 agtaaagcca agctcttttt gacggaaaca cggcttactt tgacggaaat ctgtgagaaa
    11821 gtcggctata ccgaactggg ttatttctcc agagttttta aagaagagac aggaatgaca
    11881 ccttctcaat accgtaaagg aacttttgta aaatgagaaa aaaaatcaaa gagcatgaac
    11941 tctttgccaa acttctgttc attttgttca tcggtctttt tctgcaagcc atcgtcattt
    12001 ctttgtttat ttatcatcga tcaagggatg cctatatcca gctgttcaat caatcgaatg
    12061 atgtggtact aaaaaagatt caatctgaat ttgaaagttt gaatgataca atcgagaata
    12121 cattagccgc ttttgattct aatccagctg tcaaaagcta tttttccaat gatccagccc
    12181 aacatatgga acagcttcag caactaagaa caatccaaaa aatgaatgac tctctttcaa
    12241 aaatccatcc aatgatcgat tacgatgtat tgatctttgg agaaaatgga agaacttttg
    12301 tcggaaacga catgctcact gccgtatctg ccgattcttt ttttcaatct gcgattgctc
    12361 agagagtgaa tgaacgtgct gctgatacac aaatgctttt tgcacaccac ggattgactc
    12421 ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa actgaaaaat acattgaatc
    12481 acgtttacgg atatgctgtt ttatcgatct catccaagca actagcgaat ctttttcaat
    12541 ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa cgaagaacag acgatcatcg
    12601 catcaaatga agaaaataca attgggagcc gatcaatgat tttcaaaaaa ctaacagtag
    12661 gtgaaacaac gatttcaaat ggacagcagt tgacccgtct tcctctgtac cgccaggatt
    12721 gtacattact cagtcaagtc gatcttcatt ctttatctaa ccaaatgggc gtgatttttc
    12781 cgattatttt gtataatatc gtgacacttg cttttgctgg ctcattggta tttatgtatt
    12841 tggatcgaca cacaaaatcg atctaccgct tgattgattc gctaaaacgg ctagaggaag
    12901 atggcttaaa agctactgtt cctgtacaag gaagccatga agtccaattg ctagggaata
    12961 cgatcaatca attgctaagt aatgctcagc atcattatga acagacgatc caagacgaaa
    13021 agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca gatacagccc cattttattt
    13081 ataatacgct gactagcttg aaatttttga tttggcagaa aaaaacagat gaagcagttc
    13141 aaggaatcga cagtttcatt actttactaa gaagtacgat tgggaaaaaa gaagaagtga
    13201 ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat cgatcttttg agtatgcgat
    13261 atggcgaaag aatctcggct aaaataatga ttcctgatga actgcagtca ttatctatgc
    13321 cgaatatgat ccttcaacca attatcgaaa acgcctatct tcatgctttt cacaataaaa
    13381 cgcaaggatt catcacggtg tacggcaaaa caaaaaatca gcttctttta ttcgaagtga
    13441 ttgataacgg cgatggcttc gatactgaaa ataccaaaca aaaaaatgat catttttcag
    13501 gaatcggcat cagccatgta gatgagcgga tacgcctaat gtatggcgat gcatatggct
    13561 taacgatcca atcagtgatt gggacaggaa cgactgtaac gatttgtcta ccgcttcttc
    13621 ctaaaaatca atcagaatat caaaaataaa gaatagccaa gcgctgattt ttttatttta
    13681 agttaaataa atcaatcagt attcttgact ttcatctacg cgtctcttct actgtcagca
    13741 ctggataaca gcccaatatt tgactttctc ctccgagcat cttgatttct tggagcgcat
    13801 tctctactaa tgtcattgga cggtccagca acaaatcgat cacgaaaaaa tattctccca
    13861 agctagtttt caatggtcgg gattcaatct tacttaagtt gatttcccgc cagccaaaag
    13921 ctgccagcat tttatgaagc atccctggcc gattagcagg tagtgtcaag atgacactca
    13981 tttttactgg agctgactgc tggctggtca tctttcggtc accgacgatc caaaaacgtg
    14041 tctggttcaa ctcattatct tggatattct cagctaagat ctccaatcca acatgctgtg
    14101 ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg atgttctgct acatacattg
    14161 ctgcagcagt tgttgattct gttgctacta aaggaacatt aggataatgc gtttctaaaa
    14221 actgttgtga ttgtgctagt gcttgtggat gtgaaaggat ttttgtaatc tttgttgctg
    14281 gattgcctaa aagctgctgc ttgataggaa gaacgatttc acttttaact tcgacttctg
    14341 gatgtttgga aagaagatcg attgtgtgat gaacagctcc ttccaatgaa ttctctacag
    14401 gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg caaacaaaca gggatggaag
    14461 catacgcagt caattgttct gtaggaaata gctgacaagc cgcctgataa gtgaatgaac
    14521 tttctggccc tagatagctg acgttcatat gcttttgatc ctttctataa tggtatcgat
    14581 gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt gtcgcacttt cttcatacca
    14641 agaaagtcgt tgtgccaata atgcttttat ttcttctctt gtttttccaa gagctaaagg
    14701 tcgaatagac gtttcatctt gttggatacg atcaactaga cagtccgctt ctgcctctaa
    14761 gtaaagaaca atcggctgtg tttttaaaaa acgccgattt tctgcacttt ggacaatccc
    14821 tccaccagtc gcgataattt tgtcttcatt tgacagcttt cttaaaatat ctgactccac
    14881 ttttcgaaag cttttttcac cgtaatagtg aaaatattcg gcaatcgaca attggatatg
    14941 ttcctcaatc tttgtatcta aatctacata ctctttttgt aaacattgag ctaacccttt
    15001 accgattgtt gtttttccag cgcccatgaa accaatcagc aaaatgctag ccatgagctt
    15061 ctcctcctga cacaagttct gctaaatcgt caaaaaatgc cggataagag attgaaacag
    15121 cttcagctcc ttctagttcg caaggtccat cagctatcaa agcagctact tgcagcatca
    15181 taccgattcg atgatcgcct ctgctggaca cttttgcgcc atgcagaggt gtcggcccat
    15241 ggatgatcag accgtctgct gtcgctttta tctttgctcc cattttctgt aattcttctg
    15301 ccaccgcatc aattctgttg gtttctttta cttttaattc ttctgcgtct ttgatgatcg
    15361 tgattccttc tgcttgagta gccactaaag ctagaatggg caattcatca atcagacgcg
    15421 gaatgatttc tccaccaatc gttgctttct tcaaatgact cgtcttgaca cttaaatcag
    15481 ccgattggtt ctgttcattg tgattcgtct gggtaatcgc tgcgcccatc tcctccaaca
    15541 catctaagat accagttctt gttggattga ccccgacatt ttttaacaac agttggcttt
    15601 ccggaacaag aagtcctgct gctagaaaga aagcagctga tgaaatatca cctggaacag
    15661 ttatctgttg accagttaat ttttgcggtc cggtcactat gatcgtttta tcttccactg
    15721 tgatccttcc gccaaattga cgaatcattt cttccgtatg gttacgggaa cgttcttttt
    15781 caacgattac ggatgtgcct tctgcttgta atgccgcaaa cagcaaagca gatttgactt
    15841 gcgcactggc tacaggcata ttgtatcgga taggtgataa agagcgggtt cctgtcacag
    15901 tgattggtag atactggtca ttttcttctc cttgcaagtc agcgcccatc tcgcgtaaag
    15961 gttccatcac tcgccccatt ggacgtttag acaacgaagc atccccgaac agagtagtcg
    16021 taaaaggctg tccagccaaa atacccatca ataaacgtat ggtcgtaccg gagtttccca
    16081 tgtcaattga ttcggttggt tggacaaatg agtggctacc gcgaccatga atcttgatca
    16141 cctcttcctc ttcttcgatc tcgacaccta attgttggaa ggcatgtaaa gtactcagac
    16201 aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc ttcagcaatt gctccaaaca
    16261 tgatgctgcg atgggaaatt gatttatctg ctggtatcct aacagtccct cttaatccat
    16321 gtatttgttg taataattgc atttatccag cctcctattt gatccagcaa tgataactgg
    16381 tattagacat aatgattctt tttgcttgta acaaatcctg ttggttttta aaactgattt
    16441 gcagtattcc taaaatatct tccctggtct cttggatctt caaattaatc aaagaaactc
    16501 ccgcttttcc gatcagtcca gttacttctg caataacgcc aggttcatct ggcacatcga
    16561 taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg gacaggcaac tggtctcgac
    16621 tttcttttgc ccgattgaaa aaagaaaaga tttccgattg gttctcagtc atgatccatt
    16681 ctgctatttg tgtcatttct tgttgccaat ctgccaatcg ttccaataat atctgcttat
    16741 tgctcaacaa aatatctgtc cacatgacag gatctgagga agcgatccta gtgatatctc
    16801 gaaagcctcc tgctgccagt tgtttggctc gcggatactc ttgattgaat tgttctgcct
    16861 gattgactaa gccagctgcc agaatatgtg gaaaatgact tagcattcct gttatacggt
    16921 catgttctct tgctgtaaga gttacatatt ttgctttggt tccgcaaaat agttcttgca
    16981 gtttttcaac atcttttttc gtcttttcca aaggtgtaaa gatgtaatag gcattttcaa
    17041 atagtttagc atctgctgcc tgtatacctg atttatgaga accagccatt ggatgtccac
    17101 cgacaaaccg aagatttttc tgctcagcgg atgtcacgac ttgctgcttc gtactgctga
    17161 catccgtaat gatgacgtga tcagacaagt tgattctttc aaggacatca agataagcta
    17221 aggttgtacg gacgggaacg gctaataaga taacgtctgc ttgttctgca cctgtttcaa
    17281 aatcacttgc aacgtaatca acgatctgct gttctactgc ttgctcacgg gtttttgact
    17341 gactatccca accaactact atcagttctg gattggtctg acgaatacaa agtgccatag
    17401 aagcaccaat cagtccaagc cccacgataa aaacatgttg tgccatctta tttgccccct
    17461 ttttagtaat gtttcaagta gtcacggtac tccttcactg cagctttcaa ctcaacaagc
    17521 gaatcacttg ggaattttga caatagctct gtcgctacca cagtggctac aacattttcc
    17581 gcaacgacgc tagctgcagg aactgctgta ctatccgaac gttctacact tgctttgtaa
    17641 ggttcttttg tatcgatatc aacactttga agtggcttgt acaatgtagg aatgggtttc
    17701 atgaccccgc gaatgacaag tggttcgcca ttggtcattc cgccttcgaa gccgcctaga
    17761 ttattggtac gtcggaaata gcctctttct tcttcccata ggatctcatc catcacttct
    17821 gagcctggta atgaaccaga agtgaatccg accccaaatt ctacgccttt aaaggcattg
    17881 atactaacca cagcttgtgc gattttggca tctagcttcg tgtcccattg tacgtagctt
    17941 cctaatccag ctggaacgcc tccaacgagc acttctacaa ttcctccgat cgtatctcca
    18001 tttttctttg tctgatcgat caaatctttt attctttgct caactgctgg atcaacaact
    18061 cgtacttctg acgcttcaga aatttctttg atttcttgaa tcgagatatt tccaggtatt
    18121 tctgcttgga ttccaccaag acaaatcacg tgtcctgcta cgatgatgtc aagttcttcc
    18181 agaattttct tggcaattgc gccgattgct acacgcatgg tcgtctctct tgcagaggac
    18241 cgttcaagca cattccgaag atctcgatga tggtatttca taccgcctac caaatccgcg
    18301 tgtcctggac gcggacgtgc aactcgccgc agtgattggt ctttttcagg cacttctgct
    18361 acagacatga cagttgtcca atttttccaa tctttatttt ctaccactaa agtgacaggt
    18421 gaaccaattg ttttgccgtg acggatgcca gacgtgatcc gaacacgatc tttttcgatc
    18481 ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag cgagttcgtg attgatttct
    18541 tcttctgaca gaggtaatcc tgctggtagt ccttcaataa tggctgttaa ttctggtcca
    18601 tgggattctc ccgcagtcat ataacgcatc ttacagtcct cctaagtaat ctttgaatgt
    18661 ctctgtggga acaggaacga tttttgcttg cccaatcttt tctagcaaga tgatattcaa
    18721 ttggcttcct cttgctttct tatcatgggt tatcgcgtgg aataattcat ctggatgact
    18781 tagatcgtac gtaatcggca gatggaattt ttgaagtatc ctttctaaag cagctgtcga
    18841 tccagttgga gacaagccga ttttttctgc atgagaagtg atggctacca ttccaatagc
    18901 tactccttct ccatgactga ctaccccata tcctgctgta ttttcaattg catggccaat
    18961 tgtatgacca aaatttaaaa taagtcgatt tccttggtca aagacatctt cttccactac
    19021 ttttcgtttg atttccaacg tagcagtaat gattggaaca gcatgttcca gaagatcttt
    19081 ttcgtctttc agctcgttca gctgtttcca aagcgttgca tctgctatag ctgctgattt
    19141 gatgatttca gcgattcctt ctcgtaaacg acgtaccggc aaagttttta gaacctcagg
    19201 atcaatcaat acaccagctg gctggctaaa tgttccgaca aggtttttcg ctttgtttgt
    19261 attgactgct gtcttcccgc caatactaga atctacttgt gcaagtagcg tagtcggaat
    19321 ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg aatgctgcta gatcacccac
    19381 cattcctcca ccaagtgcaa gtatcccatc gctgcgggta aaatcattat ctgctaaaaa
    19441 atcgtataac tcagctgctt gttctaaaga tttactggtc tcacccgccg gtacgattga
    19501 aaggactggt tcaaaattag cttgtctcaa ttggaacatg attttttctg catatagtgg
    19561 agcaacgttt gtatcagtga tcacagctac tttttgaggt ctccaaatcg tttgaagcca
    19621 tgatccagcc tgttctagtg cgcctttttc aatatgaata tcgtattttt ggttatcaac
    19681 atgtacagta agcattcgtt tcacttcttt cttttaaaaa gctggaatta atcgagtgtc
    19741 tgaatctttt ccattgcttc tttcatgatc gcaacttctt tcatcatgcg tgagtaagtc
    19801 ttttcattca acgattgggg accgtctgac caagcatttg caggatcagg atggatctct
    19861 acgatcatgc catctgctcc gcttgccaca cccgcacgag ccattggtgg tacaagttcc
    19921 caaattcctg taccatgact tggatcaaca atcacaggga aatgagttaa ttttttcatc
    19981 aatgggacag cacttaaatc aaacgtatta cgtgtcgctg tttcgtaagt ccgaatccca
    20041 cgttcgataa aaatgacagg agatttgtct tcgactgcaa tgtattctgc agcattaagc
    20101 cattcattga tcgtaccgga aattccacgt ttcaatccaa caggtttacc tgttttaccg
    20161 acagcagata gcagtttaaa attttgcata ttacgtgcgc caatctgtag gatatcgctg
    20221 tactcagcca ccatatcgat atgcccttca tccatcactt cagtaatcac tttcattcca
    20281 aattcatcag ccgcttgacg gatgtatttc aatccttctt cttctaaacc ttggaaagca
    20341 tatggagaag tacgtggttt gaaggcacca cctcgcagaa tttttgcgcc cccggctttg
    20401 gccattcgcg cacattctct gatttggtct aatccttcga tagaacaagg accagccatc
    20461 gtcacgaaac tgccgtcacc aatttttact ccgtctacat ccacgactgt attgccagga
    20521 tggaattctc gagaagtcaa tttgtatgtg tttaaaatac gcacagcatt ttctactcct
    20581 tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat caccttttag tccaatcact
    20641 actcgttctg ttccttgatc gattgagact ttgaatcctt ctttttcgat atgatcgacg
    20701 acttttttta tttgttcttc cgttgcttct tttttcataa ttactatcat tgtttttccc
    20761 gctttctttt gtttacttat ctatcttggc tgattttctc tactttttca aggtggtttt
    20821 acattaattc ctactaaatg accgtgtcct gttgttctca ttttcgctcc ttctttccta
    20881 cttatgaccc agataatcaa caaaaaaaga tgtccctgta ggaaattgcc tctacagtga
    20941 acatcttcaa cgagccttaa aaagcgctgc aaaaatataa aatagccact atagatgaat
    21001 ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc catcatagat acaaacctgg
    21061 tttgtttgta cccacgaagt catgaataca aacctatcta aaatagctaa agtaatacgt
    21121 attcagtttt gtaagttttc tagatttcat aaaaaatcgc tccaaactga tgtaagaatt
    21181 gtacctagta tagacataaa aaaatcgact gtcaatcatt tttatgaaac tttttttaat
    21241 tttctgatag ttaactttgt ttttcagaaa aataacaatt ggttttattg aagaaaatag
    21301 tcatgaacaa aagtatctga gaactttttt actctcttgt tcatgacata tagaataaag
    21361 cagctttgtc ttgtaaactc cgtttttcaa ataataatct aaagattgct actgaataat
    21421 aaagccgatt tcatggtcct cttcgctgct gattcggtaa ggtgactcaa catccgctcc
    21481 ccagctattg atacccccga ctcctcttac ttttgcaaaa atcgtaagaa ccgttcgtct
    21541 ttctggcggt agttcttcta tatgtgttgc ctgttccagt tcttctgctt tgaatggtaa
    21601 agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc aacgaaaaat cttcttgatc
    21661 tttatccgta ttatccaacg tttgatttct tgttagttct acccatttcg tttccatatg
    21721 catgccgcat tcttggggaa caaggtatgg ggaaacgggg agtcctgcca ctttataaat
    21781 accttcgaca ccaccagcca tccgatccgg atatgtttcc cctgataggc cttcatactc
    21841 aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc aatgcgggta attccggtaa
    21901 tcctttttgt ccataataat gcatattgac aagcatttta ccctctccag cgatttggta
    21961 ggaaattgtc acttgggttt ttggtactgt gttgatttcc agtgtaaaaa tgatttcaac
    22021 cgtttcagcg tattcattac ttgaatattg attatttact ggagcaatcg gcaaatcgat
    22081 ctttttattg tccaccagta cttctatttc tttgactttt ggatacaatc cggctcctaa
    22141 ccacataccg gatttaaatc caaatccgct acctctgtca ttatcggtcg ttgctcgcca
    22201 aaaaactgga agtgtttccc ggtatagcca ttcttttccg tttttattga gcgattccag
    22261 tccgcctctt gtataagaaa aaatataatg cgtattcccc gatttgacac ctaatgtaca
    22321 gtcgccgaag acgattgcaa ctttttccgt atccatatga cttttattta ctgtattttt
    22381 cataataata tcttacctcc tgcatggcgg gtttttcttg tccatctgca aaaacgatcc
    22441 cattccctga gaattcatag tcacttggtt tatcgtcaaa gtcaccgcca tagcgcatca
    22501 cttctctgcc tgtgattgga tcagtcactt ggatcgcctg atctttaaaa tcccatatat
    22561 agcctccttg gtacatttca taacgatcaa gcaacgtcat gtaagaatcc attccaccta
    22621 acgaattccc catgtcatgc atatattcac ataagatgaa cggtttttcc gctttatttt
    22681 caaggtagtc aatgatctct tctggtggcg catacatgca gctttttaca tctgaaatcg
    22741 aatcttcgaa tgctggattt ggaaaattcc cttcataatg gacaagtcga tcggggtcta
    22801 cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc cgcataagat tcatttccaa
    22861 gagaccaaaa aagaatactt ggatggtttt ttaatgtttc gaaattcgtt tttgctcgat
    22921 caagaacagc cgcctgccac tgaggtactg cacctggtac gttgtaagaa ggctcaaatg
    22981 cgcccatttt ttgccatgag ccatgggatt cgaggttcgt ttccgccatc acatggattc
    23041 ctgcttggtc gcataaataa taccaagtga tctggttggg gtaatgagat gtccgtacgc
    23101 cgttgatctt tgcatttttt atgatagcca aatctttttc catatcttct atagtgatgg
    23161 cgcgtccttt tttcggatgc cactcatgtc gatttacccc gttgataatc atccgttttc
    23221 cattgaagta gatgatttta tctttgattt cgattttacg aaaaccgaag gaatagtcaa
    23281 tatactccgt tactcttttt tcttcgtcta tcgtctccaa ttcaagctta tataaattgg
    23341 gctgaaaatg cccccacagt gccacatttt caaatccatc gatgcgaatc gtcatttgct
    23401 cttttaacgg atattgctgt tgatagattt ccttcccatc aggatctagt atcgctacat
    23461 tcagctgact gtttttggct acttgctcaa aagacaaatc tacttcaagt ttgccatttt
    23521 gacatttgtc atctaagcga ggtttgatcc aaagatcttc taaatggttt tgtcctcgag
    23581 caactaagaa gacatctctg aagatcccaa aaaatcggaa aaagtcctga tcttccaaga
    23641 atgcagctgt acttcttttg taaacctcca cagctagaat attttcttct tccaaataag
    23701 gtgttaaatc aaattcagac ggagtgaacg aatcttcggc atacccgata aaatgaccat
    23761 tcaaccaaat aaacattgct tgttctacac cagcaaaatg aatcgagatt tgttttccct
    23821 ttagaccttc ttctaatcga aaagtcgtca aatacgaacc aaccggattg tctttagcag
    23881 ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt gaaaggggga cggcgaaata
    23941 catgtccctc ccacggatac atcgtattga tgtaatggat cttgtcataa tttgctaatt
    24001 caatatgact aggaacagtg atttcatcaa aagaacaggc atcaaaatct tttttataaa
    24061 atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa acgccatgat ccatttaaag
    24121 actgtttaag ggaggatgtg gtttctactt cttcccttgt tgcataacaa acatgatcgc
    24181 tatgtgcatt tgattggttc actctaaaaa cttgtggatc atctaaccat tttatatttg
    24241 ctttcatact tttttccttc tctctcataa ctttttgaaa tgaagcagtt ctgtttggaa
    24301 atcaccttga aaatcgggga agtaaagtcc aactgtcatc aattcggccc ccgaaaatat
    24361 ccgcttcgag gtctgttcct gataaagtgc ctcatcatct aaccccatca gatatacttc
    24421 atggaacgct ggttgagctg aagctagtat cctccctaaa aacacaatag cttcatcttg
    24481 tttaggagac acaaacatcc atgcggcaat attttcttca aaaggactcg ccaaacgata
    24541 ataatggcca tactggatca ccggacgaat cgtttgatag gatataattt ggtcttttac
    24601 ttgctttttt tcttcaggag acagctttgt caaatccagt tcataaccaa aaacagcgct
    24661 catagctgtt tctcctcttg ttttcaacga agtagatctg tcggtctgat gatttggtac
    24721 agctgataca tgagctgtga tggacgaaat cggataagca aggcttgttc catattggat
    24781 tttcatacgc tccactgcat ccgtgttatc acttgtccat gactgcggca tataataaat
    24841 aaacccggca tcaaaccgtc cacctccacc agaacagcct tcccataaga tttccggata
    24901 atcagttgtc aaatgttcaa gtagttcata aagcccgagc atataacgat gcgctacttc
    24961 tccttgtctc tgtgggtcaa gggtaatcga atagacatct gaaagcgaac ggttcatatc
    25021 ccatttgatg tagtcaagag gaatcgtatc taaaatcttt ctcatttgtt tctcaatcgt
    25081 ctgccgaaca tctaaccttg taaaatccag tacatgttgt gatctggaag cagaaggcat
    25141 tctacctggc tcctgcataa gaaaatcagg atgtgttcga tacagttcag aatcaaccga
    25201 gatcatttca ggttcaaacc ataaaccaaa tttcaatcct ttgctatgag cataatctgc
    25261 tatttcacgt aatccatttg tcaacttgcc ttcatactcg aaccagtctc ctaatgaaga
    25321 attgtccgca tctctttttc caaaccagcc atcatctaat acgaacattt cgatgcctag
    25381 ctcctttgct tcatcaatga tcgccttgat tttttcacta ttgaagtcaa agtaagtggc
    25441 ttcccagtta ttgaccagaa tcggccgttc agcaaattga tgacgacctc ttgcgattct
    25501 ttcacgtagc aactcatggt ggatctgact cattttgttc aatccctgac ttgagtaaga
    25561 aaggatcatt tcaggtgttt gaaaagactg gcctgcattc aattcccacg aaaaacgatg
    25621 gctgttgatc ccaccgacga ctctcaattg attgatctga tctttttcaa gttcgaaaga
    25681 atggttgccg gaataaacaa attgaagtcc gatcgcttct ccagtatttt ctgtcgtatg
    25741 atgatgaact aaagcaataa agttattcat atggtgactg ctcgttcctc gacggctttc
    25801 aaatactttt gttccagata aaacagattg ccgtgaaatc tgtctttcat tgacatgtgc
    25861 tcctggcaaa gcaatgactt catcaaatcg cctagtatgg gcaaagtcta gctggaaact
    25921 agccgctttt tctagtttga ttgtttctcc ggtttcattt cttatcttta cagatcgtgt
    25981 gatcactgct ctatcagtaa atatggtata gaacaaatcg aaataaatcg ctgtttctct
    26041 atccttaagt gaaatcacca acgtttgtgc ctctgacttg tccatcacat aagtttgagg
    26101 caagccttcg atctctggct tcccatctgt aatataatgg ctgtcatatc tcagatccag
    26161 taagtctgat ccattcttgc gctgaatgat cgatgcgggc gttcgaaagt ccatgctgcc
    26221 atgagaagag tattcttgcg gtagtgtatc tttcgacaga gaccggtcag gatttaaggg
    26281 aacatttcct gaaaatccac gatctcttcg gggataattt ttattgtctt tgtactgctt
    26341 gactggtttg ccaaaataaa tatgcgccaa tacattgctt tcttctatct ttatcagata
    26401 agaaagaaat gtatttcgta aatggaagac tcggctcttt tcatcaaata agatgaaatt
    26461 tttatctgtt tttctatgcg taatatccat catttcactc ccaacaaaac ggaaaccgtt
    26521 tacttttttt tataattcct gtatactgtt tactaaacaa ttttgtcaat aaaaattttt
    26581 tatttttttg aggagtgtgt aaaaatggtt ggaattagag atgtcgcacg aaaggcaaat
    26641 gtctctcctg ctacagtttc ccgtgtgttg aataacgatc agacgatcca tgtggctcag
    26701 actactcggc aacggatagt agaggcagct gctgcgttgg attatgacat cagtactcgt
    26761 aaatatatca aaaagagaat gccttcgatt ggtgtgatct ctacgatcgg caaagcgagc
    26821 gaagaacagg atatctatta caaacatttg cgagtaggga tggaagaaga agctcgacgt
    26881 ctgcatttag ggatgaatcg aatctataat ctatctgata atcctaaaca atgggaagat
    26941 ctagatcagt taggtgcggt gatcgttgtt gggactgtta cgaaagaatc gattaccgcc
    27001 ttgttaaaac aaaacatgca aatagtcgtc gtggataacc ctgacatcca gcaagaggtc
    27061 gatatggtgt atgcagatct tgaacggatg actaaaaccg tactggaatt gttcttagaa
    27121 aatggtcata aaagaatagc ctatatcggg ggatataaag tcgatatgga tgaattcggt
    27181 gggaagcaga tttctataaa tgaaaagagg atgcgtgcct atcagcattt tatgatcgat
    27241 catcacttag aaaaatatat gtcttatcat ctaggtggct ggacagaaaa acaaggtgag
    27301 gtacttgcga ctaatctatt agaaaatgaa tcccctcttc ctactgccat tcttgtcggg
    27361 agcgatccaa tggcaattgg tgtctatcgg gcattgaaac aaaaaaatat ccaaattgga
    27421 gaagagattg ttgtagcaag ctttgataat atcgagacaa tcgcaaacct ctcccctggc
    27481 ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag cagctgttcg aatggcattg
    27541 gagcgaattg acggtatacg ggacgaacca atcattttga cttatcctgc acgattgatt
    27601 gttagggaaa gttttatccc aagaacaaaa aggtattgaa agagaggttg t
//
LOCUS       DACCQP010000031        27586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCQP010000031 DACCQP010000000
VERSION     DACCQP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.031"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(16..561)
                     /locus_tag="IVO87_001460"
     CDS             complement(16..561)
                     /locus_tag="IVO87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7449657.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(573..1265)
                     /locus_tag="IVO87_001461"
     CDS             complement(573..1265)
                     /locus_tag="IVO87_001461"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7449658.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1284..1568)
                     /locus_tag="IVO87_001462"
     CDS             complement(1284..1568)
                     /locus_tag="IVO87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449659.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1581..2144)
                     /locus_tag="IVO87_001463"
     CDS             complement(1581..2144)
                     /locus_tag="IVO87_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7449660.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2372..3034
                     /locus_tag="IVO87_001464"
     CDS             2372..3034
                     /locus_tag="IVO87_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7449661.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3158..4354
                     /locus_tag="IVO87_001465"
     CDS             3158..4354
                     /locus_tag="IVO87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7449662.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4455..5831)
                     /locus_tag="IVO87_001466"
     CDS             complement(4455..5831)
                     /locus_tag="IVO87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449663.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5868..6734)
                     /locus_tag="IVO87_001467"
     CDS             complement(5868..6734)
                     /locus_tag="IVO87_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7449664.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6823..7848)
                     /locus_tag="IVO87_001468"
     CDS             complement(6823..7848)
                     /locus_tag="IVO87_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7449665.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7866..8540)
                     /locus_tag="IVO87_001469"
     CDS             complement(7866..8540)
                     /locus_tag="IVO87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7449666.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8724..9617)
                     /locus_tag="IVO87_001470"
     CDS             complement(8724..9617)
                     /locus_tag="IVO87_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7449667.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9691..11388)
                     /locus_tag="IVO87_001471"
     CDS             complement(9691..11388)
                     /locus_tag="IVO87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7449668.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11381..12694)
                     /locus_tag="IVO87_001472"
     CDS             complement(11381..12694)
                     /locus_tag="IVO87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7449669.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12694..16212)
                     /locus_tag="IVO87_001473"
     CDS             complement(12694..16212)
                     /locus_tag="IVO87_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7449670.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPECGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16505..16963)
                     /locus_tag="IVO87_001474"
     CDS             complement(16505..16963)
                     /locus_tag="IVO87_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7449671.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17261..17779)
                     /locus_tag="IVO87_001475"
     CDS             complement(17261..17779)
                     /locus_tag="IVO87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7449672.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17781..18398)
                     /locus_tag="IVO87_001476"
     CDS             complement(17781..18398)
                     /locus_tag="IVO87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7449673.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18395..19249)
                     /locus_tag="IVO87_001477"
     CDS             complement(18395..19249)
                     /locus_tag="IVO87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7449674.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19246..20157)
                     /locus_tag="IVO87_001478"
     CDS             complement(19246..20157)
                     /locus_tag="IVO87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7449675.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20198..21532)
                     /locus_tag="IVO87_001479"
     CDS             complement(20198..21532)
                     /locus_tag="IVO87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7449676.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21740..22570
                     /locus_tag="IVO87_001480"
     CDS             21740..22570
                     /locus_tag="IVO87_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7449677.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22814..23063)
                     /locus_tag="IVO87_001481"
                     /pseudo
     CDS             complement(22814..23063)
                     /locus_tag="IVO87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23254..23448)
                     /locus_tag="IVO87_001482"
     CDS             complement(23254..23448)
                     /locus_tag="IVO87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449678.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(23953..24138)
                     /locus_tag="IVO87_001483"
     CDS             complement(23953..24138)
                     /locus_tag="IVO87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449679.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(24988..25329)
                     /locus_tag="IVO87_001484"
     CDS             complement(24988..25329)
                     /locus_tag="IVO87_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7449680.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25329..25559)
                     /locus_tag="IVO87_001485"
     CDS             complement(25329..25559)
                     /locus_tag="IVO87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7449681.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            25846..26184
                     /locus_tag="IVO87_001486"
     CDS             25846..26184
                     /locus_tag="IVO87_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449682.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26389..26628)
                     /locus_tag="IVO87_001487"
     CDS             complement(26389..26628)
                     /locus_tag="IVO87_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7449683.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26604..26951)
                     /locus_tag="IVO87_001488"
     CDS             complement(26604..26951)
                     /locus_tag="IVO87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7449684.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         8223 a         5894 c         4697 g         8772 t
ORIGIN      
        1 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
       61 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
      121 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
      181 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
      241 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
      301 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
      361 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
      421 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
      481 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
      541 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
      601 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
      661 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
      721 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
      781 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
      841 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
      901 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
      961 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
     1021 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
     1081 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
     1141 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
     1201 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
     1261 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
     1321 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
     1381 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
     1441 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
     1501 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
     1561 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
     1621 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
     1681 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
     1741 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
     1801 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
     1861 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
     1921 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
     1981 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
     2041 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
     2101 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
     2161 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
     2221 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
     2281 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
     2341 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
     2401 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
     2461 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
     2521 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
     2581 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
     2641 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
     2701 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
     2761 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
     2821 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
     2881 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
     2941 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
     3001 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
     3061 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
     3121 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
     3181 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
     3241 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
     3301 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
     3361 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
     3421 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
     3481 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
     3541 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
     3601 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
     3661 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
     3721 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
     3781 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
     3841 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
     3901 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
     3961 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
     4021 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
     4081 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
     4141 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
     4201 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
     4261 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
     4321 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
     4381 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
     4441 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
     4501 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
     4561 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
     4621 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
     4681 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
     4741 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
     4801 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
     4861 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
     4921 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
     4981 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
     5041 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
     5101 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
     5161 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
     5221 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
     5281 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
     5341 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
     5401 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
     5461 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
     5521 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
     5581 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
     5641 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
     5701 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
     5761 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
     5821 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
     5881 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
     5941 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
     6001 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
     6061 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
     6121 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
     6181 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
     6241 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
     6301 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
     6361 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
     6421 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
     6481 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
     6541 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
     6601 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
     6661 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
     6721 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
     6781 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
     6841 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
     6901 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
     6961 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
     7021 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
     7081 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
     7141 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
     7201 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
     7261 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
     7321 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
     7381 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
     7441 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
     7501 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
     7561 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
     7621 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
     7681 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
     7741 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
     7801 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
     7861 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
     7921 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
     7981 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
     8041 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
     8101 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
     8161 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
     8221 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
     8281 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
     8341 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
     8401 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
     8461 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
     8521 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
     8581 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
     8641 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
     8701 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
     8761 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
     8821 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
     8881 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
     8941 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
     9001 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
     9061 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
     9121 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
     9181 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
     9241 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
     9301 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
     9361 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
     9421 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
     9481 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
     9541 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
     9601 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
     9661 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
     9721 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
     9781 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
     9841 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
     9901 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
     9961 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    10021 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    10081 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    10141 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    10201 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    10261 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    10321 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    10381 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    10441 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    10501 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    10561 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    10621 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    10681 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    10741 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    10801 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    10861 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    10921 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    10981 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    11041 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    11101 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    11161 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    11221 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    11281 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    11341 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    11401 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    11461 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    11521 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    11581 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    11641 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    11701 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    11761 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    11821 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    11881 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    11941 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    12001 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    12061 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    12121 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    12181 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    12241 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    12301 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    12361 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    12421 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    12481 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    12541 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    12601 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    12661 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    12721 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    12781 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    12841 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    12901 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    12961 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    13021 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    13081 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    13141 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    13201 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    13261 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    13321 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    13381 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    13441 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    13501 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    13561 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    13621 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    13681 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    13741 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    13801 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    13861 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    13921 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    13981 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    14041 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    14101 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    14161 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    14221 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    14281 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    14341 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    14401 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    14461 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    14521 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccaca
    14581 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    14641 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    14701 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    14761 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    14821 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    14881 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    14941 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    15001 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    15061 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    15121 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    15181 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    15241 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    15301 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    15361 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    15421 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    15481 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    15541 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    15601 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    15661 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    15721 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    15781 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    15841 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    15901 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    15961 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    16021 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    16081 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    16141 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    16201 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    16261 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    16321 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    16381 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    16441 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    16501 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    16561 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    16621 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    16681 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    16741 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    16801 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    16861 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    16921 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    16981 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    17041 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    17101 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    17161 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    17221 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    17281 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    17341 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    17401 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    17461 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    17521 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    17581 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    17641 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    17701 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    17761 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    17821 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    17881 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    17941 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    18001 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    18061 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg ataatcgatc
    18121 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc
    18181 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    18241 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    18301 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    18361 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    18421 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    18481 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    18541 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    18601 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    18661 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    18721 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    18781 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    18841 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    18901 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    18961 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    19021 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    19081 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    19141 tccaaagcaa tggaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    19201 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    19261 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    19321 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    19381 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    19441 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    19501 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    19561 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    19621 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    19681 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    19741 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    19801 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    19861 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    19921 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    19981 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    20041 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    20101 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    20161 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    20221 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    20281 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    20341 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    20401 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    20461 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    20521 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    20581 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    20641 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    20701 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    20761 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    20821 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    20881 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    20941 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    21001 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    21061 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    21121 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    21181 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    21241 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    21301 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    21361 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    21421 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    21481 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    21541 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    21601 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    21661 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    21721 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    21781 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    21841 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    21901 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    21961 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    22021 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    22081 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    22141 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    22201 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    22261 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    22321 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    22381 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    22441 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    22501 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    22561 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    22621 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    22681 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    22741 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    22801 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    22861 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    22921 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    22981 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    23041 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    23101 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    23161 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    23221 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    23281 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    23341 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    23401 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    23461 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    23521 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    23581 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    23641 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    23701 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    23761 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    23821 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    23881 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    23941 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    24001 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    24061 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    24121 tttagaattt ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt
    24181 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca ttatactgga
    24241 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
    24301 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
    24361 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
    24421 gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa aaactagcgg
    24481 tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg caaatttctc
    24541 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
    24601 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
    24661 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
    24721 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
    24781 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
    24841 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc atcgaccaca
    24901 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
    24961 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
    25021 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
    25081 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
    25141 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
    25201 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
    25261 tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
    25321 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
    25381 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
    25441 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
    25501 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
    25561 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
    25621 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
    25681 aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta
    25741 tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat
    25801 aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa
    25861 caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta
    25921 gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg
    25981 gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt
    26041 tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa atggcaacct
    26101 cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga
    26161 ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg
    26221 cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag
    26281 atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct
    26341 gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc
    26401 cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac
    26461 ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac
    26521 tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct
    26581 acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt
    26641 ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc
    26701 tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg
    26761 gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc
    26821 acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt
    26881 ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa
    26941 cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt
    27001 cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac
    27061 tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa
    27121 catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg
    27181 acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    27241 aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc gactttttca
    27301 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    27361 gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt
    27421 ttcacaacct ctccactaaa cgatgttcaa tcagctaacc ttcggtatta gctcaaaaaa
    27481 cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt caaagcgctg
    27541 atttcacaac ctctaataca cggtgttcca gaagtagctt cttcga
//
LOCUS       DACCQP010000032        25353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCQP010000032 DACCQP010000000
VERSION     DACCQP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.032"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            96..1197
                     /gene="prfB"
                     /locus_tag="IVO87_001489"
     CDS             join(96..158,160..1197)
                     /gene="prfB"
                     /locus_tag="IVO87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7449685.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            1302..1988
                     /gene="ftsE"
                     /locus_tag="IVO87_001490"
     CDS             1302..1988
                     /gene="ftsE"
                     /locus_tag="IVO87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7449686.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            1972..2865
                     /locus_tag="IVO87_001491"
     CDS             1972..2865
                     /locus_tag="IVO87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449687.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            3017..4465
                     /gene="cls"
                     /locus_tag="IVO87_001492"
     CDS             3017..4465
                     /gene="cls"
                     /locus_tag="IVO87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7449688.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            4802..5659
                     /locus_tag="IVO87_001493"
     CDS             4802..5659
                     /locus_tag="IVO87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7449689.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            5774..6694
                     /gene="pstC"
                     /locus_tag="IVO87_001494"
     CDS             5774..6694
                     /gene="pstC"
                     /locus_tag="IVO87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7449690.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            6694..7578
                     /gene="pstA"
                     /locus_tag="IVO87_001495"
     CDS             6694..7578
                     /gene="pstA"
                     /locus_tag="IVO87_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7449691.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            7593..8399
                     /gene="pstB"
                     /locus_tag="IVO87_001496"
     CDS             7593..8399
                     /gene="pstB"
                     /locus_tag="IVO87_001496"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7449692.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            8415..9173
                     /locus_tag="IVO87_001497"
     CDS             8415..9173
                     /locus_tag="IVO87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7449693.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            9186..9863
                     /gene="phoU"
                     /locus_tag="IVO87_001498"
     CDS             9186..9863
                     /gene="phoU"
                     /locus_tag="IVO87_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7449694.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            10086..11117
                     /locus_tag="IVO87_001499"
     CDS             10086..11117
                     /locus_tag="IVO87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7449695.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            11114..11881
                     /locus_tag="IVO87_001500"
     CDS             11114..11881
                     /locus_tag="IVO87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7449696.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            11981..13549
                     /gene="liaX"
                     /locus_tag="IVO87_001501"
     CDS             11981..13549
                     /gene="liaX"
                     /locus_tag="IVO87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7449697.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            13849..14166
                     /locus_tag="IVO87_001502"
     CDS             13849..14166
                     /locus_tag="IVO87_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7449698.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            14166..14516
                     /locus_tag="IVO87_001503"
     CDS             14166..14516
                     /locus_tag="IVO87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7449699.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            14683..15621
                     /locus_tag="IVO87_001504"
     CDS             14683..15621
                     /locus_tag="IVO87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7449700.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            15636..16466
                     /locus_tag="IVO87_001505"
     CDS             15636..16466
                     /locus_tag="IVO87_001505"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7449701.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            16510..17535
                     /locus_tag="IVO87_001506"
     CDS             16510..17535
                     /locus_tag="IVO87_001506"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7449702.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            17550..18488
                     /gene="galU"
                     /locus_tag="IVO87_001507"
     CDS             17550..18488
                     /gene="galU"
                     /locus_tag="IVO87_001507"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7449703.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            18613..19053
                     /locus_tag="IVO87_001508"
     CDS             18613..19053
                     /locus_tag="IVO87_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7449704.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            19056..19658
                     /locus_tag="IVO87_001509"
     CDS             19056..19658
                     /locus_tag="IVO87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7449705.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(19721..20824)
                     /locus_tag="IVO87_001510"
     CDS             complement(19721..20824)
                     /locus_tag="IVO87_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7449706.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            21034..22035
                     /gene="ccpA"
                     /locus_tag="IVO87_001511"
     CDS             21034..22035
                     /gene="ccpA"
                     /locus_tag="IVO87_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7449707.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            22390..22785
                     /locus_tag="IVO87_001512"
     CDS             22390..22785
                     /locus_tag="IVO87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449708.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            22785..23036
                     /locus_tag="IVO87_001513"
     CDS             22785..23036
                     /locus_tag="IVO87_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449709.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            23079..23474
                     /locus_tag="IVO87_001514"
     CDS             23079..23474
                     /locus_tag="IVO87_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449710.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            23471..24727
                     /locus_tag="IVO87_001515"
     CDS             23471..24727
                     /locus_tag="IVO87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7449711.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            24739..25062
                     /locus_tag="IVO87_001516"
     CDS             24739..25062
                     /locus_tag="IVO87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449712.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            25055..25321
                     /locus_tag="IVO87_001517"
     CDS             25055..25321
                     /locus_tag="IVO87_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449713.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         8473 a         4339 c         5471 g         7070 t
ORIGIN      
        1 ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt tagactagat
       61 ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa attcgaaatc
      121 tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga cttagatcag
      181 ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt ttgggatgac
      241 agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac ctatgaccaa
      301 ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat gctgcaagaa
      361 gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt agatgaaaaa
      421 atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa caatgcgatc
      481 gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag catgctttta
      541 cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact agattatcaa
      601 gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca taacgctttt
      661 ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc ttttgactca
      721 gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct tgatgaaacg
      781 atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc aagtggtgca
      841 ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt gcctacagga
      901 acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca agcgatgggg
      961 atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga agcagctgca
     1021 cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta tgttttccat
     1081 ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt ccaagcagtt
     1141 atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct acaataagaa
     1201 ctcattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa cattgccatg
     1261 ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa atgaaggatg
     1321 taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac
     1381 aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca ttcatcaaat
     1441 tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa
     1501 tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg gtgttccaag
     1561 actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta
     1621 tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac cttgttggtt
     1681 tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag caacgtgtct
     1741 ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa ccgacaggaa
     1801 atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc aatgcccaag
     1861 gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt cggcaccgag
     1921 taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa tatggatacg
     1981 atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa acgtaacggc
     2041 tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt cggtgtattc
     2101 atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa cgttgatgta
     2161 tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga aaaagaattg
     2221 aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga attgaaaaaa
     2281 atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa cccgttatat
     2341 gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc agaagaagca
     2401 gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga tcggattttc
     2461 caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct gctgttcgta
     2521 gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca gcgtgaaatt
     2581 caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt tttccttgaa
     2641 ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac atttggttat
     2701 gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc attgattcgt
     2761 ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt gatcattggt
     2821 tcacttggct cgatcatttc tatgagacgt tttttgaaat tctaatacca caatataaat
     2881 aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct aggagaatag
     2941 aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt aagttggtag
     3001 aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc ttttgattat
     3061 gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg
     3121 ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat atattttttt
     3181 aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga aaatcggtaa
     3241 gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc ctcatccgcc
     3301 tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg aaagtacgct
     3361 ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat tcgatgcgct
     3421 gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata tttatcgcag
     3481 tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac gaggggtaaa
     3541 agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct tttttcagaa
     3601 tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc cgtatattaa
     3661 tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga aaatcggata
     3721 tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat ttggttattg
     3781 gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa accgtttttt
     3841 gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat ttttcccgtc
     3901 cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag ataccgaaca
     3961 tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg agatattgat
     4021 ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt tggctcttct
     4081 ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc tcgtttactg
     4141 ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg aaacatatga
     4201 gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt cagttggctc
     4261 agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac tggtttatga
     4321 tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa tttctacgca
     4381 tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag aaggattagc
     4441 aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat caacagatgg
     4501 agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa tggagaaagc
     4561 gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa agcaagaaat
     4621 aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat agaaactttt
     4681 aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg attttgattc
     4741 gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa ggagatttct
     4801 aatgaaaaaa cttaaattat tcttaccaat aataggatta gccattcttt tatcaggctg
     4861 ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg ctttacagcc
     4921 tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat tcatcaatgt
     4981 acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg
     5041 aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat tagtcgatca
     5101 tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg taaaagacat
     5161 cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga gtgaagtcgg
     5221 tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac
     5281 gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac aagattcaag
     5341 cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg ttgctttttc
     5401 ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga cagatgaaaa
     5461 tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa gagatcatcc
     5521 aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac aagataacat
     5581 cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg
     5641 aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa cttttacatg
     5701 ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa acctgattaa
     5761 gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa
     5821 aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg tgttggttgt
     5881 attatctatt ttttattttg tagctagtaa aggattggcc acctttttta ctgataagat
     5941 caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg gagcaaatgg
     6001 gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga cctttttatc
     6061 tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg agatctctcc
     6121 taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta ttccttctgt
     6181 cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta tttttggcgg
     6241 gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct tgccgactgt
     6301 cacaacgatg actgttgatg cattaaaggc agtaccacgg cactatcggg aagcttcatt
     6361 agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc
     6421 cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag ccttagctat
     6481 ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc ctgcttctac
     6541 attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag ttgaaaataa
     6601 tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta atatcgtgat
     6661 ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc agataaaatt
     6721 gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc agctttacta
     6781 ctttatatct tagttcgggg aataccgcac atttcatggt ccttcttgac cgaaccttcc
     6841 aaagcctacc aagaaggtgg tggaatcggg atccagctat ttaactcgat ttatttgctt
     6901 ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta tctttcagaa
     6961 tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat cttgagctcg
     7021 ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca attccagtat
     7081 ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc tcttttgact
     7141 agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc aggattggcc
     7201 ttaggtttgt cacgctggga aacagtgatg aaagtggtgg ttccagaagc gacaccaggg
     7261 attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc tgcattgatc
     7321 tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc attaagtgtt
     7381 tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat ttggaagatc
     7441 aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg
     7501 atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg attataccga
     7561 aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg aatgaaacaa
     7621 atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat atacatgtct
     7681 ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa aataagatca
     7741 ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa
     7801 tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa ggcgtagata
     7861 ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg gtttttcaac
     7921 gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg aaacagcacg
     7981 gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct
     8041 tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct ggcgggcaac
     8101 agcaacgttt atgcattgct cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg
     8161 agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca cttgtaaact
     8221 tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca gctcgtatca
     8281 gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa actaacaaga
     8341 tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat tttggttaag
     8401 acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat ctgtattatg
     8461 gaaagaaaga agcactaaaa gggatcgatc taagtttcaa tcaaggggaa atcactgcga
     8521 tgatcggtcc ttctggttgc ggaaaatcca cttatctgcg ttctttgaac cggatgaatg
     8581 acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa gacatctatg
     8641 gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc cagcagccaa
     8701 acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta aaaggggaaa
     8761 aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg tctgtttggg
     8821 atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggcggg cagcagcagc
     8881 gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg gatgaaccaa
     8941 caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta gaattaagag
     9001 agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt atttcagata
     9061 agacggcatt ctttttagat ggtcacttga tcgaatacaa cgatacgaag aagatattca
     9121 tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga taaggaggaa
     9181 acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat cagttctacg
     9241 aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg
     9301 ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac atggaaacac
     9361 gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca actgatttgc
     9421 gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc gatcatgcgg
     9481 tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg gaaatcgaaa
     9541 aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc ctagttgctt
     9601 atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa agagtcaatg
     9661 actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag
     9721 tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc ggagactatg
     9781 tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact gagttaaata
     9841 cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt tatgaaaaga
     9901 ggttgtgaaa aagctgttct acatcaagta ataagaacag tcaaacaaaa aatagctcct
     9961 catatttttc gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10021 cgggaaaggg tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat
    10081 aaagaatgca aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat
    10141 gggttattca gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac
    10201 gaataaaaga ggatcatcaa ttgattacag gaaaactgat gaactcggat caaaaaggag
    10261 aatcgattta tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag
    10321 cgagacaaat gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa
    10381 atgatggctg gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggcg
    10441 gaaaacaggg tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt
    10501 tgttgctcat ctttactaaa acagaaggga tttctctatt aaataaatct atcctgttcg
    10561 tcgttatagg gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa
    10621 tcaaaatttc agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa
    10681 tgaatcattg gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt
    10741 atcgttctgt ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg
    10801 ttatcacagt cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga
    10861 aacagttatg gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata
    10921 tccttttgtt cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc
    10981 aatggtcttt caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg
    11041 ctggcagctt aggaatagta ttatcgatcc ccatctcggt cctctttttt ctgactgtaa
    11101 ggaggctgaa acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct
    11161 acttattggc ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat
    11221 ctttttcctg ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat
    11281 aggattttta ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat
    11341 gcgtgcagct tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat
    11401 cactgatttt ttagcgatcc aaggttttac atcggtagaa ctagaagaat tatctggctt
    11461 agataagact ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct
    11521 tagtggtgct gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca
    11581 ggcaaatccg caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa
    11641 aatattggcg tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt
    11701 gatcctctgg tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt
    11761 tgttctagaa tatacgatgg ctattcttac cacattgtcc gcactaattg ttcttccttt
    11821 aacctgcgtg actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta
    11881 aaagttacaa cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag
    11941 gaaaagcttg caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat
    12001 tttagattta gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag
    12061 catggcaact gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag
    12121 tcataaagaa aaagataagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga
    12181 aatttctgag ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa
    12241 gatcttagat gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa
    12301 tgctcaattc gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct
    12361 gaatacaaaa gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt
    12421 agaggcagaa atcaaagagt tagaagctag tggcgataca ctgttagaag aacaattgaa
    12481 attagaagca gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt
    12541 tactgataag tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca
    12601 agtaggcgaa aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt
    12661 ccaaactgtt tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt
    12721 ccctgggatt gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag
    12781 tatccttgat atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga
    12841 tgtgaaagtt gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc
    12901 attctctgaa cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa
    12961 taaacgtgtg cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc
    13021 tattaaactt ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac
    13081 aaaatctacg aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga
    13141 aggtgtaaac ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac
    13201 agtaaatggt acagtgacat ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa
    13261 tggtgatgta cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt
    13321 gaacggaaat gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac
    13381 aagtcttgga agtatcaata gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga
    13441 acggacaaac caaatgttgc aattccgtcg tgtttcagat gatgagatcg cgcaagtgca
    13501 actttcaacg acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga
    13561 aagagatgtt cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta
    13621 caaaattgtg ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg
    13681 aacctgccaa ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta
    13741 tgaatatggt aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg
    13801 tcacaacgat tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag
    13861 ttgacaaaat ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac
    13921 atcgggattg atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt
    13981 ggaagtccaa ttattttata cattttatta gctttgatta tcccatctgc gcgttctgca
    14041 cgaacaaact atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact
    14101 cgtgaacaaa caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac
    14161 ttttaatgtc gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg
    14221 ttctttttcc tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg
    14281 tattgtctgt gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta
    14341 cattagtcac attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct
    14401 ccttttttgt gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag
    14461 ctatcttgat gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga
    14521 aagctgtgcc gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt
    14581 acattggtaa ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga
    14641 taaaatgata gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa
    14701 gttagccaat tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta
    14761 gaccgtgtca tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt
    14821 aattattatt cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa
    14881 cggatgatcc cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc
    14941 gcatttatta tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaatgaa
    15001 aatgggatag ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc
    15061 agttatttag atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt
    15121 tacggattag gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg
    15181 gagttgatca aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa
    15241 gatgaattaa cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga
    15301 ggaatcggga tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg
    15361 caagtccagt tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg
    15421 ggctctgaag acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct
    15481 gtcaaaacag gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca
    15541 cgtacaatgg gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa
    15601 gaaaactcag gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg
    15661 cttttgagct tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca
    15721 ttatcgctat gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg
    15781 tgacagattt tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg
    15841 ttttatttga tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag
    15901 gaggcttagc tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta
    15961 aatatcattt tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag
    16021 cacaggcaat cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga
    16081 cgcgaagctt cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg
    16141 aagggatcta tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta
    16201 ttttgctttt gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat
    16261 gttatctaat gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc
    16321 tttacttttt aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta
    16381 cattgattct atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat
    16441 taggaaaaaa tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg
    16501 agtggtaaaa tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg
    16561 gcacaagtcc tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa
    16621 gtagaagaaa tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc
    16681 gattccatcc aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta
    16741 ttcgtcattc caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat
    16801 acaaagccag tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc
    16861 tctgacatct tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct
    16921 ggaccaagcc atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt
    16981 acagatgcaa aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc
    17041 tataccaatc ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct
    17101 attggtgctg gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg
    17161 acgcgcggat tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc
    17221 tttatcggat taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg
    17281 aactggcgcg caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat
    17341 atgggaatgg tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg
    17401 atggaaatcg aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa
    17461 atcaaaacag ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca
    17521 ttaacaattt tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag
    17581 caggattagg aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa
    17641 ttgttgataa accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag
    17701 acatcttgat cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata
    17761 ttgaattaga aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag
    17821 aaacgacaga tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg
    17881 ccgttcttca agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg
    17941 atataatgga agacgaagta ccattgacac agcaattgat cgatgactat gagcgtacac
    18001 acgcttctac gattgccgtt atgcaagttc ctcacgatga aacttcaaaa tacggaatca
    18061 tcaacccagg ggaagtactg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac
    18121 cagatccaag caaagcacca agtgatttag ctattattgg gcgttactta ttgactccag
    18181 aaatctttga tgtattatca gaacaacaac caggtgctgg aaatgagatc caactaactg
    18241 acgcaatcga tacgttgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac
    18301 gttatgacgt tggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctgacac
    18361 atccagaagt aggggcacct ttgcgtgaat acatcatgtc tttaggtaaa acattagaag
    18421 cagaaggaag caagatcaat cataatccaa aaggaataac agaaatcaaa gaagaaaaga
    18481 aaaaataatc tttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc
    18541 tcgtttttat ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa
    18601 gggagtttga tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg
    18661 atacttgtcg ttttcatcgt tcgcgtattg ttgcaagttt ccaaaacttt agaaaaagta
    18721 gaccagactg tcgcagaagc aaatacgaca attgagatcg tcacacaaga cgtagatctt
    18781 ttatctagac aagtagaagg cttgctagtc aaaagtaatg agctgctctt ggatatcaat
    18841 ggaaaagtcg caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta
    18901 tcggaactta atcattctag taaaaacatc gtgacgaaag tcggcggaat cggaaaaacg
    18961 acagctaaag cgactgttgc cggaaaagtc ggtggaacag cgatgaaatt ttttaaaaac
    19021 aaaaaacata ctgaaacgac aggaggaaaa taagaatggc taaaaaaggt ggatttttat
    19081 taggtgcagt agttggaggt acagccgcag caatcgccgc attattgtta gcacccaaat
    19141 ctggtaaaga acttcgggat gaattggctg ctcaagcaga tgatttgatg gacatggctt
    19201 cagattacac agatatggca aaagaaaaat catctgagtt aacaggtatt gcaaaagaaa
    19261 aagcgcaagg tctttctcaa caagcagggg atctagcagg caatgttaag cagaaggtaa
    19321 gtgaatcagc agaggaagca ggaagtgaat cagatgatat cttgttaaac ctcaaagaac
    19381 aatcagctga tttatctaat cgtttcaaaa aaacagcaga agatctgaaa aagccagcag
    19441 acgaattagg tagtattgct aaagatactt cagaagatat catgatcgat gtaaaagatg
    19501 ctgcagataa agcaggagag acggtcaatg aaggaatgtc agaagcaaaa ccagttgcag
    19561 atgacacaaa agaaatgttg aaaatggcaa aagaagatgc aaaagaagct aaaaaagaaa
    19621 tgaaagatac aatgacagat tcgactacca atcactaaga ttactcattt ctagaataaa
    19681 aaaggtgaga gcggaccagc tctcaccttt tttattttca ttactctaaa acaagtaatt
    19741 cttttggtgt agtcgtgaat ggcacacaac catcacttgt cacatacaca caatcttcga
    19801 tacgtacccc tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt
    19861 cttcaatcac taagtcgttt ccttctacaa gagaagggaa ttcgtggacg gttgttccga
    19921 ttccgtgacc taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc
    19981 gtgcgatttg atcaagttca cctgctgtca cacctgggcg aactgcctct tgtgcggcaa
    20041 gctgtgcttc aagagtgatg ttatagattt tttcttggaa ctcagttggt ttttgataag
    20101 caactgtacg agtagcatca ctgcaatagc cattccaaat aacgccaaga tcgaataaca
    20161 ctagttcatt tggtgaaacc ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg
    20221 ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac accttgtttt tttaattggt
    20281 attcgatttc tgcgataatt gcttgttcag ttacgccttc ttttactgtt ttgaagccaa
    20341 tttcaaaggc aacatcggcc catttaccag cttcgatcag tttgtctttt tcttctaccg
    20401 ttttgatcag ttgcagacgc tggataatag aagttagatc ggtgtaagaa tctgtacctg
    20461 tcaagatatc agagatagca tcgaagcgat cgacagtcag agcattcttt tcaataccta
    20521 cattaccagg ttgcccatag cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt
    20581 cgctgtctaa gtagccacgt acatcgtacg cccagctgct gttttgagca tcttcaactt
    20641 ccagagcagg agtgaaaagg aaaggatctt gttccagagg gataaaaaga gctagtacac
    20701 gctcgtgagg atcactttca tatccggaaa agtaagcgat gtggccaggg tcgctgatat
    20761 aagccaattc tacttggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat
    20821 tcatttggca acctactttc taaattaatt ttgtattcaa taattgtacc actgattgat
    20881 taaaagacca acgttcttaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa
    20941 acggttgcaa ttgttgagat tatttgatat cctattttag aaatgtaaga cgtattttca
    21001 gaaaacatat aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat
    21061 gatgtagcga gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat
    21121 ccaaacgtca aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat
    21181 cgtccaaatg cagtcgcacg tggcttggca agtaagaaaa caacaacagt tggggtaatc
    21241 attcctgatg tcagcaatat gttctttgcc tctttagcac gtggtatcga cgatgtggca
    21301 acaatgtaca aatacaatat catcttagca aactcagatg gcaacgatca aaaagaagtc
    21361 aatgtattga ataatttatt ggctaagcaa gtggacggcg tgatctttat gggtcaccat
    21421 atcacagacg aaatccgtgg agaattttca cgttcaaaaa cgcctgttgt actagctggt
    21481 tccattgatc cagatgaaca agtaggtagc gtaaacattg attatgtgtc agcaacaaaa
    21541 gatgcagtaa ataaattagc aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg
    21601 ctgattgatc ctatcaatgg tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa
    21661 aacaatctac catatagcga aggattgatt tttgaggcac aatacaactt taaagatggt
    21721 ctgtctctag ttgaccggat ccataatagc ggtgcaacag ctgtctatgt ttcagacgat
    21781 gaattagcca ttggtatctt ggatggttta ttagaccgcg gcgtaaaagt accagaagaa
    21841 ttcgaaatca tcacaagcaa caattcttta ctgacagaag tagctcgtcc tcgtttgaca
    21901 agtatcacac aaccattata cgatttagga gcggtatcca tgcgcctatt gacgaaaatg
    21961 atgaataaag aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa
    22021 ggatcaacta aataataaaa gatcaatcat ttaaaggttg ttccaaaagt ggtcaacttc
    22081 tggaacaacg tttatacgag aaagggggtt atgacaaaat tttgtcataa cccctttttt
    22141 tttgtgactt taaaaaaaga gaaaatggcg gaaacaaacc ttacgtccat cgtatacgac
    22201 acaatctgtg cgtagatgct acattttgtt cactactatc aaaattactt taaaatgaaa
    22261 acgtgataaa aaagaaagta gaaaagtaga taagtaactc taaaaaatcc aaaaaaatcc
    22321 aggctcgatt gcgtacttat cttaaaggag aaacaatcca tcatatataa cccgaaagga
    22381 gacttctatt tgcctaagaa aagacgaaag accatagtca tcattttgac cattcttact
    22441 ataggaatcg gagggaaatt ttacatggac aaaagagaaa tgcaaagaca gaaagattta
    22501 ctcgcagtag aaaaacagtc cgtcaaagtt ctgaaaaata cctttgcgga tatcaaggaa
    22561 gtgaagattg aaaaatctgc tcgaaatgaa atgacgggtt cataccggat agttattcta
    22621 atgactaata aacaagatca atcgatttat ttttcttata gtttttggaa agaaagaaat
    22681 gagataggaa gttacggaat agttgatgaa aaaaagcaaa aagaaggaaa tacgctcaat
    22741 aaagttaaag taacttactc taatggaaat gaggaaagta tttaatgatt actgacaaac
    22801 aatatagtga attaagtgat gctgtttact ggctagatcc taaacataga gactatgtac
    22861 ctgaaatgca agaaaattta agttttaaaa ttaacggcac tatgtataaa atcctaaaaa
    22921 tcaaaaactc cttcgacggc atgcaggcga tggctgtagc gccaatcgtc catagcaaat
    22981 tggagaaaaa tttcaaaaat aaaaaaattc ctgcaaactt tcgcgtactt aaataatgaa
    23041 ggtgtcaata cttacataaa aagaaaggag gcacgttctt gtcaaggaaa ataagaaaac
    23101 tcagcttagc cattttatta atcgtcatat tagggatcgg agggaaagtc tatatggaca
    23161 aaagagaagc acaaaggcag aaagatttac tcgcagtaga aaaacagtca gtcaaagttc
    23221 tgaaaaatac ctttgcggat atcagggaag tgaaggtaga agaatttaaa aaaaaccctg
    23281 ttaccggaag ttatggtgca cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt
    23341 ttgattatag tttttggaag gagagaaatg aacttggtgg ttttggcatt gagaacagag
    23401 cggtacaaaa gacaggagtc actataaaaa gagtaaaagt aattttttct aacggacagg
    23461 aggagttagt atgacaataa acgataagca ttacaatgat attagtgaga aggtttattg
    23521 gctagatccc aaatatcccc gctacaatga gggatacaaa aagaactctg ttaaagaatt
    23581 tgctggtatg gaatttcaaa tcctacaaat caaagactca ttggacggca tgcaggcgat
    23641 gtctgtagcg ccaatcaaga acggtaaagt ggatctttct caagtagtga ttgcttttgc
    23701 aggaacgaac ttaagtgatt ggaaagattt ggaaacggat gcaagaagta tcatgaatct
    23761 ctacgggaat agaccaggag caagtttttc tgaaaaagca acaacaaaca agattttatc
    23821 cgttagtgga ttgaaaacag cggggcaaat caattcagcg attgcatttg ctaatgaagt
    23881 aaaaaaggaa tatggagaag ctgctatttc tactaccggc cattcattag gtggattctt
    23941 agcattatat gtagcagcag agaaacaatg gaaaaatgtc ggcttcaacg ggccggaccc
    24001 gtatgatctc ttatcaccag aagcaaaaaa atgggtggaa gaaaatcccg gtatgctcac
    24061 caattaccga aatcacgccg attttatcgg taattttggt ggtaatggca caggtgctga
    24121 agttcggatc agtatggaaa cagcattctt gaatctgctt gcttcccaca gtttaacctc
    24181 ttggaagttt gatgaaaatg gaaagctgat tattcctgaa aatgaaaaca atgttaaagc
    24241 tcgaaaacaa cagaaagaaa accaagcgac ccagttgttt tcctatcagt tgcaagaact
    24301 gaacaacata cgtaaaaaac tacgattaag tggcggccgg ctgacagtta atgaacaaat
    24361 ttacttagat gacacacaag ctaggttagt agtggaaaag ttatcggcaa aaatgaaaag
    24421 cgctatggaa gaagttatcc gcatcaatca aagaggaatt tttgagctag agaaaaaatg
    24481 gcaagaaggc ttgcggcaag cacgtgttgt agccccttcg ttgactgaac aggaaatcat
    24541 agaagcatta gctagtgtgg gcgccacaaa agaaaactta gtcgattctg cgaaagaagt
    24601 ctatctgcta cgaatcaata aggcaagacg tatgggggag gcctttgata cattggcgaa
    24661 ggagatccaa gacaaaatca atgaaatcgt ggcacgagat cgagaattag cacagcaatt
    24721 caattaaagg agaagtagat gaaactagaa gaattggaaa gagattatcg aagtgaacga
    24781 agacaattag aagctcaaga agatgaattg atccacttta agaatcaagg attacgattg
    24841 atggagcaag aggagcagcg actgcatcat gtattaaaga acttagcttt agataatgag
    24901 cctctgtttc aagcaagacg aagttattcg aattatcagg agcattatca acaagcccta
    24961 caagtagaac gacgaaaact ctatcagcag caagatgaac tagaacgttc ctatcgtcaa
    25021 aaaatcagac aaataactat ggagaaatca tcttatgaat gaatttttaa tgcaattact
    25081 aaaagaatta gagaaaaatc tacatcgatc aaatgtacgt tcagttggta tggggatatc
    25141 tcagcaaatt tgggcagaaa aacgattgac agatcaaaac taccaaatga ctgcagaaga
    25201 agctgctcaa gaattttcta accgacatgt acacttgaaa ggatcagtaa aagggcaatt
    25261 tgcagaaaaa ctaactgaat tattccaaag acaagaaaag aaattaaaac agtttcgtta
    25321 gtaaagttat atagaaaaat tttgtgaaaa tgg
//
LOCUS       DACCQP010000033        24979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCQP010000033 DACCQP010000000
VERSION     DACCQP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.033"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(526..1233)
                     /locus_tag="IVO87_001518"
     CDS             complement(526..1233)
                     /locus_tag="IVO87_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449714.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1298..2134)
                     /locus_tag="IVO87_001519"
     CDS             complement(1298..2134)
                     /locus_tag="IVO87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7449715.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="IVO87_001520"
     CDS             complement(2152..3561)
                     /locus_tag="IVO87_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449716.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="IVO87_001521"
     CDS             complement(3575..5398)
                     /locus_tag="IVO87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449717.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="IVO87_001522"
     CDS             complement(6241..6567)
                     /locus_tag="IVO87_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449718.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="IVO87_001523"
     CDS             complement(6613..6975)
                     /locus_tag="IVO87_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7449719.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7226)
                     /locus_tag="IVO87_001524"
                     /pseudo
     CDS             complement(7012..7226)
                     /locus_tag="IVO87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7353..7688
                     /locus_tag="IVO87_001525"
     CDS             7353..7688
                     /locus_tag="IVO87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449720.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7764..9761)
                     /locus_tag="IVO87_001526"
     CDS             complement(7764..9761)
                     /locus_tag="IVO87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449721.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9859..10134)
                     /locus_tag="IVO87_001527"
     CDS             complement(9859..10134)
                     /locus_tag="IVO87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449722.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10366..11100
                     /locus_tag="IVO87_001528"
     CDS             10366..11100
                     /locus_tag="IVO87_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449723.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCSNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11139..12281
                     /locus_tag="IVO87_001529"
     CDS             11139..12281
                     /locus_tag="IVO87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7449724.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="IVO87_001530"
     CDS             complement(12376..13767)
                     /gene="sufB"
                     /locus_tag="IVO87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7449725.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13772..14242)
                     /locus_tag="IVO87_001531"
     CDS             complement(13772..14242)
                     /locus_tag="IVO87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7449726.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14229..15464)
                     /locus_tag="IVO87_001532"
     CDS             complement(14229..15464)
                     /locus_tag="IVO87_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7449727.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="IVO87_001533"
     CDS             complement(15461..16747)
                     /gene="sufD"
                     /locus_tag="IVO87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7449728.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="IVO87_001534"
     CDS             complement(16760..17533)
                     /gene="sufC"
                     /locus_tag="IVO87_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7449729.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17730..18578)
                     /locus_tag="IVO87_001535"
     CDS             complement(17730..18578)
                     /locus_tag="IVO87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7449730.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18602..19288)
                     /locus_tag="IVO87_001536"
     CDS             complement(18602..19288)
                     /locus_tag="IVO87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449731.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19281..20315)
                     /locus_tag="IVO87_001537"
     CDS             complement(19281..20315)
                     /locus_tag="IVO87_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449732.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20710..21048)
                     /locus_tag="IVO87_001538"
     CDS             complement(20710..21048)
                     /locus_tag="IVO87_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7449733.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21050..21406)
                     /locus_tag="IVO87_001539"
     CDS             complement(21050..21406)
                     /locus_tag="IVO87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7449734.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21412..22599)
                     /locus_tag="IVO87_001540"
     CDS             complement(21412..22599)
                     /locus_tag="IVO87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7449735.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22790..23635)
                     /locus_tag="IVO87_001541"
     CDS             complement(22790..23635)
                     /locus_tag="IVO87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7449736.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23869..24834
                     /locus_tag="IVO87_001542"
     CDS             23869..24834
                     /locus_tag="IVO87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7449737.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7311 a         5245 c         4240 g         8183 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tctttttacc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgttc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat
     7201 cggctgagat tacttacttt tttcatgatt ctcccctcct gttttattat atacaacaat
     7261 atggtgtatt tcaactcata aaatataaaa aacttcaaat accattacaa atacaacaac
     7321 ttgttgtact atactgagat gggggtgtat taatgaaaca gaaacaatat gcaaaggtca
     7381 ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga attagctgaa aaaatcttct
     7441 ctacacaaca ggcagtagca agatgggaaa attctgtaac agagcctaac ctcgatagct
     7501 taacagctct ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgtcg
     7561 tagattatga ggaagagttt ttggctctct atcgttctct tagcactgaa gatgcagttc
     7621 gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga
     7681 aagattgaaa aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag
     7741 attaagagct atggataacc tgtctagttt aatacaattg ggtcttttct cttccttact
     7801 ctccgtccat catctcttgt tacacttgta aattgataca gaggaactat cccagctcct
     7861 gataatatca attcttttat tgtttcatct atccctgccc ctttcgctaa atatattaaa
     7921 aaactactac ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga
     7981 gtgaatcctt gtttaacaaa gtagcctctg ttttttatta aagcattttt cacgctgtta
     8041 tccccaacat tacgtaggcg ttcacaaaca gtgttaataa gttctttctc tgtcattaga
     8101 tatgtccctt gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt
     8161 ttttccttgc tgagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg
     8221 ttaaaacata tcttaaccca ttcataagaa aaatattgat atgtaaaatg aattttttcc
     8281 agaagtggaa ggatatcttc attcaatttt tgaatcacag aagttgcatt aaaaacgtct
     8341 aatcgataga cattgcttct cttatgatta aagtttccta acgctttctc ttcctttaac
     8401 ctatttaggt attcgagcat cttcaaagga acatcttcat catctaattt ttttatcaat
     8461 cctataaaag tcataagtga tagtaactta ttacatttat ctatgtcata agttttacca
     8521 tagtaagaac tcattttttt agctaattgt tcccccgaca aaatacaagt cacactttcc
     8581 ttactatcat caaaaaaatt aacatcagaa agcaagacat catagacacg tcgcatttcc
     8641 tctagataat tccttattgt cctgaaaagt ttgtctttca ttgattcaag agtgtcaaaa
     8701 agtacttttg attcctcttt caattttcga aaatgtgtag gatacaagtc taggttctct
     8761 aatagccaat taaatgtttc tacccgactg tctttcttta aaattagcat cacaactcca
     8821 ataaaatttt cactgatatc acaattactg gaatgacaat aaaaaatgga atgcccagtt
     8881 ttactggtaa aaatactagc agagggatta tgatcgttgt gaaacagaca tcttaagttt
     8941 ttacttgact taagtaattt attcatatca atggttaaca atctttcata aatctcatta
     9001 aaagacatgt cggaggaatc aaacaggcag tctttaaacc actgtttcat ttcttctttg
     9061 tcgtcatttt ttatcaactt aacaaaatcg gtttcattga ctagttcata ctctctccct
     9121 gaatcacttt gacaattttt agaacatgtc atggtaaaat caatcggaag ctcagtaata
     9181 tctaacatgt tgtcttcatt aattattatc actgcatctg acttatatcc tccaaaaaac
     9241 attctattcg aagcatttac attgtcatca cacccgggga tgttcttttt caagtattcg
     9301 taaacagcaa ccattttacc atagtcagta attgcttctt ttaatagaaa gacaatcttg
     9361 tatttttcta gattttcaga gtaccgaata cttttttgta tcgcaaacgc agaattatga
     9421 actaaattga tagataaaac cttttctatg cttaagtatc tactatcatc tttactcaaa
     9481 tctactacct tccccttctc atctttaaca gtgttatcga catccagaat aattactctg
     9541 gtttccacaa aactctcctt gtcattgcct ttattctttg tcttgcttgg aataaatgat
     9601 tgtgatgaat ttagtatctc tacaaactca tctcttgtaa cttctctata gttatctaaa
     9661 cacctatctc ttacatcagt tttttctttt tttgtaactg gcttctctaa taatttttct
     9721 tgatctacat aaattggaat attaatcatt gatgtttcca tttgtgtttc ctccttataa
     9781 aattctgtta aagactaaaa catacagata ataaaaacaa actctttatt actatatgtt
     9841 ttagtcttat ttgtttactc aacctttata ccgttccatg aaactatcaa tatcagattt
     9901 tttaaatcta atgaccgtat caatttttat aacaggtaac ccgtattgag aaacatattt
     9961 tctaatcgtt cctactgata cacctagata ttcacttgcc tgctgatagt ccatatactc
    10021 tagagtgact ccagaaataa tgtcttctag cataggcttt agcactgtaa ttatctcttt
    10081 cacttgttgt tcaacttgta tagaaacaat gctatcaatg atttgtagag acatatttcc
    10141 ccctcctttc gtgattgctt gaacaaattt tctaagaatt atcttcgtat tttttgctca
    10201 aaataaaagt attttgttat taaccgatta atcaaacaaa aaatgattac cagttcatcg
    10261 acatacacat aatacctcga taaaaaaggg gtgtcaacag ctttgtaaaa tttatttaca
    10321 acaccccttt tttgaggtat actaatttta ggaggtgtgg tcaatatgat aaaacgtcca
    10381 ccaataaatt atttagaacg aaaaaagata ctcggaacaa aaataaaagc tataagaaag
    10441 agtaaaaaat taacacaacc agcatttggt cttatgatta acaacggtca attaattgac
    10501 aaaaaaacta tctacgaatg ggagaaggga acctatttac caataccaga gcgattatcc
    10561 cgaattgctg atttgggaaa tatgagtata gaggagttag tttgtagcaa tgtagaagaa
    10621 tatattttgg gaattattct ttatagagat agcattgtac tagatggtat aacatttcca
    10681 gataaaaacc tgttccagca tcttcgtcaa cagttcccac cagtacactc taatttagat
    10741 acatggctgg atagatattc caaattagaa ccagagatgc aagaatttat tgctaacaag
    10801 acttgtaata aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa aatagaagaa
    10861 ttattcatca atgctattgt agaagaattt gacaataaca ttcttttcct cactagcagt
    10921 atagaagaac tactagaaag aatggtagat gaatggctac ctattcaatt gaaagacatg
    10981 tcttatcccg aagaagctgt gagagaaatt actgataata taaacaagct agaacaaacg
    11041 atttcatcaa ttgggaaaaa atacactaag aaaaaaatga agggaggtga taccatctag
    11101 tggtaagtac tccttgtaaa ctaagaggag atgatttaat ggcaaaaata tcaaagtaca
    11161 aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca cattgccacc gatccacgaa
    11221 ctggtgaaaa aataaatact acgagaagag gttttagtag caaacaagaa gctcaaaaag
    11281 attatgacga gtacagacat caaatattat acggtgttaa aaagaaagca cctgatatga
    11341 catttgaaga cctgtataat gaatggataa ctcatcagcg taccagtgtt aaagcttcaa
    11401 caatcgcaat ctcagtaaga tacgcaaata atcaaatact tccagcattt ggtaagctta
    11461 aactatcgaa tatctctgtc ccatattgtc aaaaagtggt agatgaatgg cacagtaaat
    11521 atgaaagtta cgattatatg agaaaacaga ctgcacaaat tttacgctac ggggttgcaa
    11581 tgcaatatat tgacaataat cctatggaaa aaacacttct tccacgaaaa aaagagtatg
    11641 agaaaaatcg taaattttac tcaaaagagg agcttaataa tttactggat gcttttaaag
    11701 attttggtaa catgaaacaa tacgccttct tcagattatt ggcatacaca ggtatgagaa
    11761 agagtgaagt actcgcttta cagtggaaag acatagacac gtttaataaa gaactacatg
    11821 tcaataaaac tcttgctgta gatgaatttg gtaaagttat aattcaatct ccgaaaacaa
    11881 gagcttctag acgagttatc tctttagata ctgagactct ttctatcctc aacaactgga
    11941 aattacagca aaaagaagag tatttaaaac ttggatacaa tactagctct aaagagcaac
    12001 atgtttttac aacagttaaa aacactctat acattccaaa cactgttaat gattggttaa
    12061 gatatatttt gaaaaaatat aacttgcctc gtattacacc tcatgggttt agacatacac
    12121 atgctagttt attattagaa gcaggagaat ctgtcaaggt agttcaacaa cgattagggc
    12181 atgaaaatag taaagtgaca ttagacatct atgcacatat tacaaataat gcacctaaga
    12241 aaacaggtca agactttgct gatatgatgg ctcatcagta aaagagctgg tcaaaatctg
    12301 gtcatttcta gtatacaggc aaaacaaaaa agctagaacc cttatatatc aagggttcta
    12361 gctcctataa caggtttacc caacactgcc ttccatctca taactgatca atcgattgag
    12421 ttcgactgca tattccattg gaagttcttt ggtgaatggt tcaacaaagc ccatgacgat
    12481 catttcagtt gcttcagatt cggtcaggcc tcggctcatc agatagtaga gttgttcttc
    12541 tgagattttt gagactttgg cttcgtgttc taaagagact tggctgttgt ggatctcatt
    12601 gaatgggatc gtatcggatt tcgaccattc gtccatgatg atcgtgtcac attcgatatg
    12661 agagatcgaa cccgcactgt ttttggcaaa ggttacttgt cctcgatagt ttacttctcc
    12721 gccgtctttt gaaattgatt tagagacgat agagctagat gtatttggtg cattgtggat
    12781 catttttgca cccgtatctt ggatttggtt tgctcctgca aaggcgatgg agagcatggt
    12841 gcctcgtgca ccttttccat ctaagtaaac acttgggtat ttcatcgttg tttttgcacc
    12901 taggtttccg tcgatccatt caacggttgc gccttcgtat gcttttgctc gtttggttac
    12961 taagttgtac acattgtctg accagttttg gatcgttgtg taacggcaat acgcatcttt
    13021 tcttgtaaag atttctacga tggcggcatg caggctgtta cttgaataag taggtgccgt
    13081 acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca atgatcagcg tacgttcgaa
    13141 ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt aatggaacat cgactttgac
    13201 gccttttggc acgtaaataa aggtcccacc tgaccatacg gctgaattca gcgctgctaa
    13261 tttgttgtcg gttggcggta caagttttga aaagtactct ttgaataatt cagggtattc
    13321 tttcaaagca gagtcagtgt cagtaaagac gattcctaac ttttcgaatt catctttcat
    13381 attatggtac acgacttctg attcatattg tgcggaagca ccggctagat aagctcgttc
    13441 agcttctggt ataccaatct tttcaaatgt atctttgatt ttttctggta cgtcttccca
    13501 gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg atggcatcga aatcgatgtc
    13561 tgaaaggtcg gggccccatg tttgcattgg cattttgtta aatgtttcta gagatttcag
    13621 acggaattca agcatccatt ctggttcgcc tttgattctt gacatctcac ggatcacttc
    13681 ttctgtcaag ccttctcctg tactgaaaac cggttcgaca tcatcgtgaa acccgaattt
    13741 atattcttct aattctggta cgcccatgtt ctcactcctc ttccttgtgg taatgatgga
    13801 aagctgcggt accatcgttt tccattgctt ttcctagtgc tttccaggca agtgtcgcac
    13861 atttgatccg agcgggaaat ttagctacac caccaagcat agcagcatcg cctaattggt
    13921 cgggatcttt gacttctttt ccttggacaa gctgtagaaa gtctacagtt aaatgttctg
    13981 cttcttctat tgattttccg atgacggcgt ctgtcatcat cgaggcactt gccgtactga
    14041 tggagcagcc actgccgtca aaggcgatat ttttgatgat tccgtctttt acatcgactt
    14101 gcaattcgat cacatctcca catgtggggt tgttcaattc gatcttgctt gtcgagtcag
    14161 tcaagcttcc acgatgatgc ggatgggaag aatgatccaa gatcacttga cggtataaat
    14221 tgtctaattt agatagtccc atgttggaaa aactcctttg ttgccatgat tgcttcaact
    14281 aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc tcgcgcgagc agtcgctgat
    14341 acttcaaggt acttcaatag cggttgcgca caatgatgac ctgctctgac ggctacccct
    14401 tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc catccaaatt gaatgcaacg
    14461 actcctgtcc gaaaattcgg gtcttgtgga ccataaacag tcaatccatc aattgcttgt
    14521 aattttggca acacataagc ggctagttct tcttcatatc gatggatatt gtccataccg
    14581 agtttttgca aataatcgat tgcatggcct aatgcgatag cgccggcgat atttggtgtc
    14641 cctgcttcga atttccaagg tagttctttc catgtactgt cgtataattc gacaaaatcg
    14701 atcatttcgc caccaaattc aacaggttcc atttcttcta accattttcg tttaccatat
    14761 aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc tgaaagcaaa gaaatcacag
    14821 tctagtgctt ggacatcaat ggccatgtgc ggggcagatt gagcaccatc tactacgatg
    14881 accccgccgt tttcatggac aagttcagct aattcttgaa caggattgaa gacacctaag
    14941 acattggata catgagcaat ggagacgatt ttggttttgt ccgtaatctt tttcgctgca
    15001 tcctccatat ccaggaagcc ttcttctgtt agttcgatat atttcaagac agcacctgtt
    15061 cgttttgcca actgctgcca cgggataaca ttagaatgat gttccatgta agagagcact
    15121 atctcgtctc ctgctttgat atagcggtcg ccgaaggttc tggctaccca attcaagctt
    15181 gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt ttgcatggat gaacgcccgt
    15241 actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt ctgctaacgt atggacaccg
    15301 cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta atgcctggag tacttgtttt
    15361 ggcttttggg tcgtcgctgc attatccaga taaactaaag gttcatcatt gacgtcttga
    15421 aataagatag gaaaatcatt gcgtagcttt ttagcatcga tcatccattc aacttccctt
    15481 cgattacttc gactaattct ttccgaactt gttctacagg gattgctgtt aagacagaac
    15541 cgaggaatcc tcgaatcact aagcgctctg cttcttcttt atgcaatcct ctactcatca
    15601 gatagtacat ttcttctgga tcaactcgtc ctacactggc agcatgtcca gcagttactt
    15661 cgttttcatc gatcaaaaga ataggattcg catcaccacg tgctttatca gaaagcatca
    15721 agacacgact ttcttgttgg gcatctgcac cttttgcccc tttcaagatg tggccgatac
    15781 cattaaaggt caatgttcct ttttctcgga tcactccgtg ttgcaaaata tgcccgatcg
    15841 tatgaggggc tttattggtt actcgtgtat cgattccttg gatttgtctg cccgagctga
    15901 ttgccacgat tttgacttct gcgtgtgcac cttctcctgc taaatctgag tcaaagtcag
    15961 cgatcacatg accgtcattc atgacaccaa ttgcccagtc gacagaagca tctcgtaaaa
    16021 tgtgcccacg gcgattcata taagaagtaa tattttctcc taattgatct acggctgaat
    16081 atttgatttt tgaaccagct ttagcgatga cttccacgat gatattacct gatgattttg
    16141 ctacttgttc tttggttgtc tggaaacgtt ccagataaga aaattcacta tgttcatctg
    16201 ccacgatcaa cacatgcttg aagaagtgct catcggatgc gccatcttga atgaataagg
    16261 actcgagtgg ttcatcgatc acgacatttt taggcacata caggaaaata ccgctgttca
    16321 taaaagctac atggagtgca gtcaattggt cttcgttcat gtttactgct tttttcatat
    16381 aatattcttg gactagttct ggatattcga tcattgcagt gaacaagtcg gtaaaaatga
    16441 ctcctttttc tgctaattcc acaggcagtt gttcaaaaat cgtccatgaa ccttgttgga
    16501 caagcacagg attgtctttc attgcatcaa atgccggaat cgtgcctgtt tcactgatgg
    16561 tttgtgtttc tttcacatca aataacgacc agcgatggaa tttcacccga tcgatatgcg
    16621 gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg gaaggttctc atccaatcag
    16681 gttcgccttt acttaaagaa aaatcattta ccgcgtcaag ataatcgagc cagttttttt
    16741 ctttcattct tgctccccct tacgcttctt cttctttata gtcgattcct aattctttac
    16801 tgataccagc gtagccttct gcttccagac gtttagctag gtcagcattc cctgtcatga
    16861 ctactcggcc ttccatcatg atgtgaacga catcaggtgt gatatagttc aataatcgtt
    16921 gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg catttcattt actcctttag
    16981 caacgacttt caaagcatcg atatccagac ctgaatcgat ttcgtctaaa attgcaaatg
    17041 taggttctaa catcaacaat tgcaggatct cattccgttt cttttctccg ccagaaaaac
    17101 cttcgttcag ataacgttca gccatttctt ctggcatatt tagtaaagcc attttttcat
    17161 ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt ttcatcacgt ttcgcattga
    17221 tcgctgcacg catgaattcc gcattcgtga tacctggtat ctcacttgga tattgcatag
    17281 ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa gtctaaaaca tttttcccgt
    17341 caaacaaaat ttcgccttct gttacttcat agtttggatt gcccatgata gcggcagata
    17401 aggtagattt tcctgtgccg ttaggtccca tgatggcatg gatttctccg gttttcattg
    17461 ttaggtttac gcctttaagg atttcttttt cctcgatcga cacatgtaga ttttttattt
    17521 ctaagacaga catgcattat acccccaaat tctctttcgt tctcactcat tttagactaa
    17581 ttgagaatga agcgcaatga ccttccttac taaaatcaaa tatcttttat ttattattaa
    17641 acaattttga tttctaaatt agaataggta taaaaaagat gagaaataag ccattacaga
    17701 cttatttctc atcttttttg ttccatacct tattttgcat cagcctttac tggtttaaca
    17761 gagccgcccc atttgtctgt gatccattct tggacttctt tgcggtgaag cacttcaatc
    17821 agttttttga ttttgtcact gtcttcatca cctttacgag tcgcaataat attcacgtaa
    17881 ggagagttgt ccttttcaag caatacggca tcttctaatg gattcaaccc tgcgccgtaa
    17941 gcgaagtttg cgttgattgc aactaggtca ccttcatcat tttggtacgt agaagctaaa
    18001 agtgatgggt cgatcgtatg gttgaacttc aagttcttag ggttttcttc aatatcatcg
    18061 aatgttgctg tttccagatc tactccatct ttgactttga ctaagccagc atcttgtaga
    18121 attgtgatga tgcgtcccca gtctgattca gaatttgaag taatgattgt tgcaccatct
    18181 tttaattcgg aaacatcttt gactttttta gagtacaagc ccattggttc gatgtggaca
    18241 gcaccggcat tgacaaagtc atatcctttt tctttgattt ctttgttcaa ataaggaata
    18301 tgctggaagt aattggcatc aatgtcgcct tcttccaaag attggttcgg taatacatag
    18361 tcatcgaatt tgacgatttc aagatcgatt ccttcatctt tcaataatgg tttcacatgt
    18421 tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt tcaatgtcgt gtcgtctgct
    18481 ttatttgctg aatctttgct gtcgctgttg ttaccgcatg ctgctaatcc tacagtcagc
    18541 aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat cctacatccc cctatatcct
    18601 attatctttt gtctgttttc ttagttaaga aatcaccgat tccttgaacg ataaatacga
    18661 ttagtaaaat aattaaagtc gcaacgaatg tcactgttag attaccacgc tggtagcctt
    18721 cctgccaagc aagacctcca agtccacctg cgccgatcgc gccggccatc gcagtaaatc
    18781 cgatcatcga gatcgttgta actgttaatc cagaaatcaa tgcgggaata ctttcaggta
    18841 tcaagacctt ccagatgatt tcccagtaac ttgctcccat tgcatctgct gcttccagta
    18901 caccagggct gacttcacga aaagcaatct ctactagacg ggcataaaat ggtgctgcgg
    18961 ataaaacaag agctggaatg gctgcttgcg cacctagcat agtgcctacg attgctttag
    19021 taaatggaat gatcaaaacc atcaggatca taaatggcgt tgaccggaaa atattactga
    19081 tgatcgatac gattccataa atgattcgag catacggttt gtttttgcgt ccaatagagt
    19141 aaaggatcaa tcccaaaact aatccgatga tcgtcactaa gaccattgag attaatgtca
    19201 tatatagtgt atcaaggata gcctgattca tcgtttccgg gttgatttgt ttaaaatcta
    19261 gatatgtttc tgaaaagctt ttatccacga tggatcacct caattcctac tccttgttct
    19321 ttgatgaaca cttctgcttg atctaattct ttggtttcac cagtcatcaa gacagtcaat
    19381 gaaccaaagg aaccttcttt cacttgtttg atctttccat acacaacgtt gacatcgatg
    19441 ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg catttctttc gctaaaggtc
    19501 aaagttgcca aacggccgct tggattttct ttgatgagtt cagctaatag ttctgtcgaa
    19561 tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg ttacttcttt tttcggttgt
    19621 ctgaagacag tcaatacgtc tccttcttct acgactttcc ccaactccat cacagcgact
    19681 ttgttgcaga ttttgcggat cacattcatt tcatgtgtga tcaagacgat ggtcaaattc
    19741 aatttcttat tgatcgctaa caataagtct aatacttctt ctgttgtttg cgggtccaat
    19801 gcgctagtag cctcatcaca aagcaggatc tcaggatcat ttgccaatgc tcgtgcgata
    19861 ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat atgcttgtcc gcgtccttct
    19921 aacccgacta aacgcaacag ttcttctgcc cgttctttac gtttgctctt tggtacgcca
    19981 gctaattcca gcggcagctg gatattttca agtacagttc ttgaccataa caggttgaaa
    20041 tgctggaaga tcataccgat tttctttcgg aatgtacgta attctttgtt ttttaatttt
    20101 gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg gaagctcaag tccattcagg
    20161 agacggacta atgtactttt tcctgcgcca gaatagccga cgattccata aatatcccct
    20221 ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt ttccgttttt cccattgaat
    20281 gtctttttga cattcgttag ttggattaat gacatgccat tcacatcttt ctcttatgat
    20341 ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa aaggacgaaa gcttgaactt
    20401 ccgtgttacc accttgattt accgttactt cgcaataaca gcctcttcca gtacttggac
    20461 ttagcttctt tccgtatact gtcgcgctgt aacgggcact cccgtagtaa gtttgcttac
    20521 gctcgcttac tctgctctaa gaccatcttc catctcattt tcgttaccca ttttcagcaa
    20581 acagggctct cttcaaacgt ccgtgacatg tactcttctt ttcaaagctt tctcgtttca
    20641 tataggattg cttatgatta tagcaactgg aagaatcagt gtcaactttt ttcaagtgat
    20701 tttctgattt tataattcgt caaattcttt tggatcaaca tctttgaaac ttaagatcca
    20761 cgcattcatt tcatctgaat cattcaagat attgatatct tcaccgattt tatcattaac
    20821 agaagaaact gtacccgtta gcgggctggc aaattcattg accgcttttt ccgcttcaac
    20881 ttcgatcaga ggttcatctt tttttagcga ttgtcctgct tttggcagat tggcaaacgt
    20941 gatgtctcca agttcttctt gcgcttcatt tgtcaaacca acacagtatt cttttccatt
    21001 aaataagatc caaaggttgt cctttttctt caaatgcttg cctgccattt tatatcagtt
    21061 ctccttcata aattttttca ttgaatccaa ttgcaacaag ctggtcctct cgtaagatca
    21121 gtggtcgctt gatcagcata ccatctgtcg ataagacttg gctcgctttt gaagccgtaa
    21181 actcgcctac ttgatctttc agtccaagtt cccgatattt catcccgctt gtgttgaaca
    21241 ggcgacggat tggtacttga ctcgtttcta gccaagtctc aatcgttgag gccttcggta
    21301 cttctttgat catatcaacg gtttgataac taacgccatg ctggtctaac cacgctttgg
    21361 cttttttgca tgttgaacat ttcgggtacc agtaaaaggt gtacaatttt cttacctcct
    21421 tgaagatctt gcaggtgctt cattttggta tctcatcgat aatatcaacc cgataccgat
    21481 catgttccct aaaatcgagg atcctccctg actaatgaaa ggcaatggga tccctgttaa
    21541 aggaagtaat ccgatatttg ctccaatatt ctcaaagaca tggaacagca gcatcatgat
    21601 caaaccgctt gcgatatagg cgtaaaattc attattcgta tcgaaacaca cccgaatcat
    21661 acgatagatt aaaataaaat acaagaaaat gacgaatgca ccaccgataa agccgaagtt
    21721 ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca ggcacataga tattgcttac
    21781 gttgaatcct ttaccaaaca agccaccggt accaattgcc aaaagtcctt gggccggctg
    21841 ataactcatt cctgacgtat catggaaagg gtccagccat gaatcaatac gtgcaaactg
    21901 ataagatttg aatccaactt tataaagcaa gtctcttccg ccttcagtcg taaccaagaa
    21961 gatcgttcct gctccaacga ctactgccaa gattactaca ggtgcgatga tgcgccaaga
    22021 gattccagac atcaggaaaa tcccgccaaa gatcgcaaga aagacaagca tcgttccaaa
    22081 gtcatcttgg gccatgatca atgctaaaac cggtattgtc acgatcaaga gcttcccaat
    22141 cagccaaaag tccgatttca tcgtccgttc ttttacttgc gtattatgtt tagttacgat
    22201 cagtgccatc atcaagatgt acgcaatctt catcaactct gctggctgga aagtaaacgc
    22261 accgaatcga aaccagtttt tagaccctgt tgaagcagct aaattctgat catagaattt
    22321 gagcaagagt cccattacca ctaaccccaa accataaagg taaggagtca gtttccagat
    22381 ccatttggaa ttcaaatgca tgatgacaac gattgctgcc gctccaacta aataccagac
    22441 aacttgcatc cctacgcctt tcaagactgc tggattgttc ggatcatggc taagtgctac
    22501 atataatgag agcagaccaa tcaaacataa cagaaagact ggtaaaatca cgccataatc
    22561 aatacgatta tcgttcgaag cttttaattt tttttccatg tttcatccct tttcttaaaa
    22621 accatactgc tgattattat agcggtccag ttagataatg gaaagtcttt ccatagacat
    22681 ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc aaatgcagta caaaagccta
    22741 ctgcttatct ttagtgtatc atagaacttt ttttgataca tgcaattatc taccagctga
    22801 gattctcctc gctgtgagaa gtttctgtgc tttcattgta tcgaaggatc tcttcggcaa
    22861 tctctagcag ctgatcagaa accagattgg ttcgttggat gaaataaaaa tatcgatgga
    22921 aattcaacgg agtcattggc acattttctg gaacagagcg cttagacaaa atcgagcgac
    22981 cgattccttc cttcagcaac gcaacaatta catcgttact tttcacgatc atcttcttat
    23041 cttggatatt catctcttca aaataacgct gcgtgtaatg aaaaacacca gagtttgctt
    23101 ctcttacaag ccacaatttg ctttttgggt ccccagcgat gactagctgg tcttccatca
    23161 aagagtatct ttgtacgccg gaagtctcta atggcttttc gataaaacca aaatcggatt
    23221 catgtttcgc tacttgttct aatgattcat gagaattcgt gatttcgatc tcaaaatgca
    23281 ctttaggaaa ccttttgatc agatgtttca tcaactgagg caaaagatag acagcaaagg
    23341 tgtgggaagc aacgatccga catacttctt cttcattttg ttcattgagc aatttttgct
    23401 gagtttcttc ccattcttct aacaaaacca gtgtccgttc atataataag tcggcttgct
    23461 tagttggaag catctctttt cttccgttac gttgaaaaag atcgatattc aattcccgct
    23521 ccaactgttt gatttgattc gagactgctg gttgagataa aaacagtact tctgctgctc
    23581 tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt taataactta aacatggcac
    23641 cacttccata actttttatg ataattacta taaaaactat ttgatttaaa tatacaaaaa
    23701 accatctata atagaaatgt acaacaaaac agttgctgat ccaactagta acttagatga
    23761 agacaatcag ctgtctgctt gctcgtaatc atacattcct attcatttta caatgtattc
    23821 gttcatcttg gtactaaaat ctattaaata aaaaggagaa tattatatat gaagaacttt
    23881 ttgaaaatta ttccgatccc gatctgcggg ttgatccttg ggttagcttc ccttggaaat
    23941 ctgtttaaag actatcacca tgttgcttta ggcaatctaa ctggcggtat ctctatgatt
    24001 cttatgatat tcatttttgt taaattgttt atcttattcg aacacacgaa acaaacgttg
    24061 catgatccaa tcatcgcctc cgtttcacca acttttacga tgtcactcat ggttatttgt
    24121 acttattttg tttcatatga aagtattgca ccaattgtca aattcatttg gctgtgcgct
    24181 gttatcttcc aagtgatcct tgtcttttat ttcaattatc accatgttat caaagctgat
    24241 ctttcaattg aagcaattta tccaagctgg tttatcattt acgttggatt tggggtcatc
    24301 actgtaactg ccgggaactt cttcccactt attggaaaaa ttttcttttg gatttcattg
    24361 atctgctatg cgttcttatt gccgatcatc atcaaacgtg tctttttcgt aagaaatatg
    24421 gctcatccga cacgtccgct gattacgatc gttgcagcac ctggttctct ttgcctaaca
    24481 ggttatctta aaaactttgc tgaacctaat ttttatcttg tcgtagcatt gttcgcttta
    24541 tctcaagtat tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa gcttcgtttt
    24601 tatccaagct atgcagcctt tacattcccg cttgttatct ctgcaacagc tatatttact
    24661 actgttcatt acttcaacag tttaggtatc tatagtagcg tattggattt cttaaaagtt
    24721 gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta cgtacgtttc
    24781 ttaattaaag aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc ttaataatcg
    24841 attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc aaacaaaaac
    24901 agctgttcat gtttttgtac tggttgggct tattaccgca gatgcaagct tttgaacacc
    24961 gtttaccaag aggtcgtaa
//
LOCUS       DACCQP010000034        24336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCQP010000034 DACCQP010000000
VERSION     DACCQP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.034"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(132..2477)
                     /locus_tag="IVO87_001543"
     CDS             complement(132..2477)
                     /locus_tag="IVO87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7449738.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="IVO87_001544"
     CDS             2756..3658
                     /locus_tag="IVO87_001544"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7449739.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="IVO87_001545"
     CDS             3869..5644
                     /locus_tag="IVO87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7449740.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="IVO87_001546"
     CDS             complement(5710..7296)
                     /locus_tag="IVO87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7449741.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="IVO87_001547"
     CDS             7547..7771
                     /locus_tag="IVO87_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7449742.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="IVO87_001548"
     CDS             7913..9664
                     /locus_tag="IVO87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449743.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="IVO87_001549"
     CDS             9664..11448
                     /locus_tag="IVO87_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449744.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="IVO87_001550"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="IVO87_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="IVO87_001551"
     CDS             12732..14618
                     /locus_tag="IVO87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449745.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="IVO87_001552"
     CDS             complement(14659..15561)
                     /locus_tag="IVO87_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7449746.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="IVO87_001553"
     CDS             15791..17194
                     /locus_tag="IVO87_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7449747.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /gene="ascB"
                     /locus_tag="IVO87_001554"
     CDS             17194..18627
                     /gene="ascB"
                     /locus_tag="IVO87_001554"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7449748.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="IVO87_001555"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="IVO87_001555"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7449749.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="IVO87_001556"
     CDS             19911..21638
                     /locus_tag="IVO87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7449750.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21711..22004
                     /locus_tag="IVO87_001557"
     CDS             21711..22004
                     /locus_tag="IVO87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7449751.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="IVO87_001558"
     CDS             complement(22098..22958)
                     /locus_tag="IVO87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7449752.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="IVO87_001559"
     CDS             23050..23478
                     /locus_tag="IVO87_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7449753.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="IVO87_001560"
     CDS             23640..23816
                     /locus_tag="IVO87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7449754.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="IVO87_001561"
     CDS             23843..24289
                     /locus_tag="IVO87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7449755.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4352 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaatt
//
LOCUS       DACCQP010000035        24090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCQP010000035 DACCQP010000000
VERSION     DACCQP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.035"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            210..3488
                     /locus_tag="IVO87_001562"
     CDS             210..3488
                     /locus_tag="IVO87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7449756.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4029..5936)
                     /locus_tag="IVO87_001563"
     CDS             complement(4029..5936)
                     /locus_tag="IVO87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7449757.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6066..6941
                     /locus_tag="IVO87_001564"
     CDS             6066..6941
                     /locus_tag="IVO87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7449758.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7021..7995
                     /locus_tag="IVO87_001565"
     CDS             7021..7995
                     /locus_tag="IVO87_001565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7449759.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8184..9320
                     /locus_tag="IVO87_001566"
     CDS             8184..9320
                     /locus_tag="IVO87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7449760.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9457..9801
                     /locus_tag="IVO87_001567"
     CDS             9457..9801
                     /locus_tag="IVO87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7449761.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10072..11359
                     /gene="efmA"
                     /locus_tag="IVO87_001568"
                     /pseudo
     CDS             10072..11359
                     /gene="efmA"
                     /locus_tag="IVO87_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11497..12096)
                     /locus_tag="IVO87_001569"
     CDS             complement(11497..12096)
                     /locus_tag="IVO87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7449762.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12500..13621
                     /gene="mnmA"
                     /locus_tag="IVO87_001570"
     CDS             12500..13621
                     /gene="mnmA"
                     /locus_tag="IVO87_001570"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7449763.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13867..15288
                     /gene="arcD"
                     /locus_tag="IVO87_001571"
     CDS             13867..15288
                     /gene="arcD"
                     /locus_tag="IVO87_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7449764.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15302..16420
                     /locus_tag="IVO87_001572"
     CDS             15302..16420
                     /locus_tag="IVO87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7449765.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16480..17772)
                     /locus_tag="IVO87_001573"
     CDS             complement(16480..17772)
                     /locus_tag="IVO87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7449766.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17922..18482
                     /locus_tag="IVO87_001574"
     CDS             17922..18482
                     /locus_tag="IVO87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7449767.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18611..18988
                     /locus_tag="IVO87_001575"
     CDS             18611..18988
                     /locus_tag="IVO87_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7449768.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18921..20993
                     /locus_tag="IVO87_001576"
     CDS             18921..20993
                     /locus_tag="IVO87_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7449769.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21204..22151)
                     /locus_tag="IVO87_001577"
     CDS             complement(21204..22151)
                     /locus_tag="IVO87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7449770.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22397..22861
                     /locus_tag="IVO87_001578"
     CDS             22397..22861
                     /locus_tag="IVO87_001578"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7449771.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRT
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22923..23966)
                     /locus_tag="IVO87_001579"
     CDS             complement(22923..23966)
                     /locus_tag="IVO87_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7449772.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7825 a         4409 c         5058 g         6798 t
ORIGIN      
        1 acgactttgt tttatgctct tttttatacg ttattttaaa aaaacttatt ctttttacgt
       61 taataaatct ctttattatc aacaacttaa gttatatttt ttctttttat aaaatacact
      121 tattataatt ttgtttttta ttgacagtaa aaataaatta ggtaatactt taagtaaacg
      181 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
      241 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      301 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      361 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      421 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      481 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      541 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      601 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      661 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      721 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      781 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      841 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      901 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      961 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
     1021 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
     1081 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
     1141 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1201 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1261 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1321 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1381 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1441 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1501 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1561 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1621 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1681 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1741 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1801 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1861 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1921 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1981 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     2041 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     2101 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     2161 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2221 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2281 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2341 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2401 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2461 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2521 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2581 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2641 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2701 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2761 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2821 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2881 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2941 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     3001 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     3061 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     3121 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3181 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3241 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3301 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3361 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3421 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3481 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3541 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3601 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3661 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3721 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3781 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3841 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3901 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3961 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     4021 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     4081 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     4141 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4201 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4261 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4321 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4381 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4441 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4501 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4561 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4621 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4681 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4741 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4801 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4861 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4921 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4981 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     5041 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     5101 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     5161 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5221 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5281 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5341 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5401 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5461 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5521 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5581 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5641 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5701 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5761 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5821 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5881 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5941 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     6001 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     6061 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     6121 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6181 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6241 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6301 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6361 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6421 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6481 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6541 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6601 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6661 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6721 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6781 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6841 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6901 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6961 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     7021 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     7081 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     7141 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7201 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7261 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7321 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7381 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7441 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7501 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7561 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7621 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7681 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7741 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7801 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7861 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7921 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7981 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     8041 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     8101 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     8161 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8221 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8281 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8341 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8401 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8461 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8521 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8581 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8641 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8701 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8761 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8821 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8881 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8941 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     9001 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     9061 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     9121 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9181 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9241 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9301 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9361 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9421 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9481 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9541 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9601 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9661 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9721 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9781 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9841 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9901 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9961 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
    10021 atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
    10081 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
    10141 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10201 gaaaccggtt cggcgacaat tttggagttt tgctacttta ctaggaatga ttcccatggt
    10261 tttactaagc ccatttgtag gaccgctagt ggatcgctgg gataaaaaag ctcttttgat
    10321 tgtcacggat attattgtag cgattttcgc gcttatttta gcagtagtcg gaacgatttc
    10381 agaatccttc ccgatttggc tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac
    10441 attccaaatg ccaacgatcc agtcgattat gccgacaatc gttccatctt ctcacataac
    10501 aaggacgaat ggacagttag gaatggtcca atcagcgaat ttcatcattg cgccagctct
    10561 aggtgcggct ttgttttcag tcgttcctgt caattacctt attttattag atgtattggg
    10621 ggcagttttt ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat
    10681 attggaagtt ccgcttacta tttttaaaga tgcaaaattt gggctgcagc aattgatgga
    10741 taataaaggg ttatggtaca taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc
    10801 agcaatcagt ctgtatccat tgatgacgct agattacttt ggtggttccg ttggacaggc
    10861 gggagcagtg gaagtagtct acgcagtcgg aatgctctta ggcggtgcac tgatcagttt
    10921 tataggaaca tggaaagacc gaatgaaacc tattattata gcctacatca ttatgggcct
    10981 gacgatcggt gcaagcggat tggttccaaa cgatagtcaa gggttcttgt actttcttat
    11041 cttgaatgca ggagcaggtt gtgcaacgcc atatttcaat acactgctga tggcaatgat
    11101 ccagcagagt tatgaatcga atgttttagg tcgtgtctta gggaacttca actctttgat
    11161 gaacctggca ggtccaattg gattattatt tgcaggacca ttagctgatc gtttaggggt
    11221 agaaaaaatg ttcttgtttt ccggaatcgg gattttatta tgcggaatcg ttttattttt
    11281 aacgtcagct gcccgaaatt atgataaaga attacagaaa aaactggtaa aagaacacca
    11341 tgagcaaaaa gacgaatagt atggaatgga gttgtgacag aagtcttgtt acaactccat
    11401 ttctgatcct aatgttcctg tggcaataag cctactcaaa tgaaaaatat gagtaattat
    11461 ctttatttgt ttaatacttg atatagggca gttttttcac gacctatcgt acttcctctt
    11521 tgcttcatag atttctttga tttgttgctc agataacgga gtgccaaatg gtagtttttc
    11581 ctttaagagt tctctttccg gtgcgtctac gcctacattg acatcgttct cgataggtac
    11641 gctgtaatga tggatatgtc catgaagatt gcggatagtg tcgttaggcc cgagcaacat
    11701 cggataatga gtcaagtaat attgttgatg gtcgaattta acgatggcac ctacatcata
    11761 aaaataaaat ttgggacttc cgtcagtaag cctaggatca tttttttcta aataacggaa
    11821 aaaagcgcga ctgtcatggt tccctttgat caagtggatt gttccttgca gttgagtgac
    11881 cagttccaaa atttctgcat ttgctttttc atattgcgga tgcatagcaa ggtcgcctag
    11941 atgataaaca tggtcggttt cttttattac tgcattccag ctagtgatca actgttggtt
    12001 catttcatcc acatttgcaa agagccgcgg agcaaagtcg ttttttccta ataagtgttc
    12061 atggaaaaag tgcatatcac tgataaagta attcatgtat cttttgcctc ccttttttct
    12121 tagagtaaac aaaatcactt atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa
    12181 atgagacgtg cagctttaag cgataagaag caattttgaa aaatagtttt tcacaattta
    12241 taaaattatt ccttattgcc gaaaggctgg ccctttttat gagtatgcca attttttata
    12301 tcgaatgctc aaaagatgag tgattttttg gttgactatc atgtttgtgt actgaatagg
    12361 tccagtagaa cagtattttt acgacttgat aattaaggga tgaaattttc agaaatatga
    12421 cgtataatgt aaaatactga aaagttgcga ccttcaactc gtagatgttt cagaatctaa
    12481 ttgaaatgat ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag
    12541 cggcggtgtc gactcatctg taacagcact tttgttaaag gaacaaggat acgacgtgat
    12601 cggaatattc atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca cggccacgga
    12661 agattataag gatgtagcga aagtagctgc tcaaatcggg attccttatt actctgtgaa
    12721 ttttgaaaaa gaatattggg atcgtgtatt tgaatatttc ctagccgaat accgcgcagg
    12781 acgtacacct aacccagacg tgatgtgcaa caaagaaatc aaattcaaag cattcttgga
    12841 ttatgcaatg gatcttggcg ccgattatgt agctactgga cattatgcgc aagtcactcg
    12901 cgatgaaaac ggcgtggtcc acatgcttcg tggagtagac aataataaag atcaaactta
    12961 cttccttagc caattatcac aagaacagct ttcaaaaacg atgttccctc taggcggcat
    13021 ggaaaaatca gaagtacggg caattgctga acgcgcaggc cttgcgactg ctaagaaaaa
    13081 agattcaacg ggaatctgct ttattggtga aaagaacttc aaacaatttt tgagcaacta
    13141 tctgccagca aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag gacaacatgc
    13201 tggcttgatg tattatacga ttggtcaacg tcaaggacta gggatcggtg gcggaggaga
    13261 ttcgcaagaa ccctggtttg ttgtaggaaa agatctagcg acaaacacat tatatgtggg
    13321 gcaagggttc catcatcctg cattgtatgc cacaagcttg gatgcaagtg aaatccactt
    13381 tacgacgaat gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc gttatcgtca
    13441 gcaagacgtt cctgtgacag ttcgcttatt ggatgataat cgtgcagaag tcgtattcga
    13501 cgaacctgta cgtgcaatca ctcctggaca agcagttgta ttttatgatg gaatggaatg
    13561 tctgggtggt ggtttgatcg accacgcata tcaagaaaca aaagttttac aatacgttta
    13621 agcgatatta aatgaatcaa aacagctatg gctaacaaaa gaaacacctt ttcttttgga
    13681 tagcatagct gtttttttgc aataaaatta atttttattc attttatact tgaaatactt
    13741 gtgtaaaaag ttcataatat ccaataatat gaataaagtt gctttttgtt aagcaagagc
    13801 agttgcaaaa tagaagggaa tcttttgcaa gtgatttgtt caattttata agtgagggtg
    13861 atggaaatgg aaaagaaatc gacgggtatt agcaaatggg gactggttgc tctggttgtc
    13921 agttcgtcaa tcggaagcgg tgtgtttggg atcacaagtg atctggctag ttctgcagct
    13981 ccagggccag cgatcctttc atggataatc gttgggattg gtattttagc tttggtatta
    14041 tcgctgaatc atttaggaga aaagcgtcca gatctagatg gtggtatttt cggttacgcg
    14101 gaagcttcat ttggtaaatt aggtggcttc atcagtggtt ggggttactg gttgtctgcg
    14161 tggctgggaa atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga atttttcccg
    14221 atattcaaag gtggacaaaa tgttccttct attttatttg caagtatttt tatttggggt
    14281 ctgactttat tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg
    14341 atctgtaaat tagtgccatt attcctattt ttaatatttg tatttatcgc atttaaagtg
    14401 aatatattca ctgctgattt ttggggtaac gtatctgata atctagtgaa tggaacaggg
    14461 cagcaaggaa cgatctggtt gcagattaaa ggttgtttga tggttatgat gtgggtcttt
    14521 gtagggatcg aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc agaagcccag
    14581 caagcatcga tcattggtct gctttgttta ttgtttatct atatccttgc ttcgatcttg
    14641 ccatacggcg tcttatctca agaagagtta gcaactattt cacaacctgc tatggcaaat
    14701 attttaaaag gaatcgtggg aaattgggga gcagcgctga tcaatatcgg cttgattatt
    14761 tcgatcattg gttcttggtt gtcttggacg atgctcccag cagaaacaac gatgttgatg
    14821 gctcgagacg gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact
    14881 tattcgttag ttgttactgc aatcttgact aatctattct tattgacttt cttagttact
    14941 gattatgcct atcaatttgc ctattcttta tgtacagcgg caattttgat ttgttatcta
    15001 cttgtcggga tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg
    15061 atcggtggga ttgctgtagt ttttgaattg atgggcatca ttttagcagg attttcttat
    15121 gtccttttat gtagtattgc ctatcttcct ggtttttgct tctactggat cgcttgtcgt
    15181 gaaaccaaac atcaaattac gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc
    15241 gcactgcttt caatcgggtt gcttgctgta ggctggatca agatataagg gggaaagaaa
    15301 catgaaaatc gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa agaaagcggt
    15361 atatgatatt gcacaaagct cgatcgaagc attgacatta gacgaactcg ttggcttaga
    15421 tgggactagc gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg actatggctg
    15481 gatcgaagga tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc
    15541 ggaaaacatc ttacaaacga atggagcgac tggtgctaat cttttagctc tatacgcgtt
    15601 agtggaagca ggagaccatg ttgtttcgat gcttcctact tatcagcaat tatatgacat
    15661 ccccaaatct ttaggtgccg ctgttgactt tgttcatttg aaggaagaag aagattggca
    15721 gtttgattta gaacagctag aagccttagt caaacctgaa acaaaaatca tctgtttgaa
    15781 tagtgcaaac aatcctacag gcacattgct agatcggaaa actttagaaa aaatcgcaga
    15841 aattgctcgt acagtagatg catatgtatt gatagatgaa gtatatgcgc ctttaacgga
    15901 taaaggtgaa ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct
    15961 atccaaaacc tactccattc caggtattcg tattggctgg acagctactg gacctgagtt
    16021 ggcagaagtt tttcgaaagt atcgagacta cacgatgatt tgcggcggag tcctgtcaga
    16081 tgatcttgct gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat
    16141 cattacagag aaccttgtga ttttaaaaca atgggtagcc aatgaaccga aagttgaact
    16201 tgtcgctccc aattacgtct ctacttcttt cattaaatta gccatcgaag aagatgacca
    16261 gacattttgt atcaatttat tagaagaaac aggagtacta ttagtaccag gaagtgcctt
    16321 tgatttaccg aagcatgccc gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg
    16381 attattcctc ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca
    16441 gtctgtgaca actgttcaag cagtgttttt catttacgct tattgtctat gagaaggaga
    16501 gtgccctgaa gctatggaga ctcctgaagc tttttcttct gcagtttctt gctgatggat
    16561 ctcttgtgta ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag aagtcgaaag
    16621 aaagacgcca cgataaccaa atgcactgga aacactggag ccgatcatag gccctactac
    16681 gttaccagtt gcttggaaag attggttata actgaagatc cgtccagcag cttgatgagg
    16741 cgaatatttt gtgatcaatg cttggacagc agggagcaga caagcatcag agataccaat
    16801 caaaaaacgt aaggcagcta attgccaaac attcgtgacg aatgccattg ggatataaac
    16861 cagaatcgca aaacccaaac caatcgcaag tattctttca ctcccaatct tgtcacctaa
    16921 gcgaccaaat cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag caatgattcc
    16981 gctgatcaac gtaacattcc catgtccatg cagcaattgg cgaatgtaca gactgatgat
    17041 tggactaatg gaattgttgg atgcttggat gatcatcgtt gtgacgaaca taccgatgac
    17101 gacgtgtgga tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt tttctttttt
    17161 cactgggaca aatttttcat ggacgaaaaa cagactcaac aagaagacga aaaataagat
    17221 gatccctgta atgaaaaagg tagggcgata gccaaacgct gaagcagaga tcccgccaag
    17281 aagcggtcca agtaatgttc ctgtaacaga accagttgct aaggttccaa gaacttgacc
    17341 acttttttct tttggcgtcc cagttgcgac aagggcagtg gcattactga tataaccaga
    17401 aaaaatccct tgaagcagac gcaatgcgac taattgatac acactagtca cgcaacccat
    17461 caaagaaatg acgattgcca ttcctaatga agcacgcaac agcatcaatt ttcttccttt
    17521 ttggtcggct aattttcccc accaaggaga gataagcgta gtcaccaaga aagtagatga
    17581 aaaagtcaat ccgctccaaa agttcaattg tgacgtacta taattaccta acgtatctat
    17641 gtaaagtgac ataaaaggca tgactaaact aaatccgata ccagccataa atgttccaaa
    17701 ccataagaca aacaagtttt gttcccaagt ttctcttttc ctaaataatt tttcctttat
    17761 tcttgcagcc aattagactc cttctttctg aaaattttca tctacagctc tttattataa
    17821 actacaatat gtagtttatc aatgaaagtc tcttttttct ctttcttttt cgtggtaaac
    17881 tgaaagagtg aaggatttat tttatgagag gaaacatgga aatgaaaaaa agaaatctgt
    17941 ctcaagagaa aatcatcgac tgctttcgag aactagcaga agagatggat gttcagcaaa
    18001 taacctttca gcatttagcc aaacgcttgg atatcaaggc tccctcgctg tataaccatt
    18061 tcaaaaatat tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag gaacttaatg
    18121 accagctgcg acgggctctt gtgggaaaaa gtggtcagga agcactgcag atctatgcag
    18181 aaacttatcg atcctttgct ttttcaaatc aagcagtgta tgagttactg atcagtgtac
    18241 cgcataccaa tgaagctgtt ttattagatg gaatccatga aacgaatcag atcattttgc
    18301 agatatttga ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc
    18361 gtagtatgat ccacggttat ctttccttac gttttttagg atactttaca aaagaaccaa
    18421 caatcgatcc agaagacagc tatttttgga tgatcaataa ctttatcgct actttgcctt
    18481 aaacaatcag attcatttgt aacaatttta agatgttcat taaatgaccc ttttataaga
    18541 gaaaattctt gcaactttct tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag
    18601 gagcacgagt atgtatcaag taattaccat gttcggggac aatgagcctt ggtggttttt
    18661 tgaagactgg gaagaagata ttgaagaaga agaaacattc gagtctttcg aagaagcacg
    18721 acaagcgtac gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa
    18781 aagtaacttt ctcagcgcct tttggaatga cggagatgag cgctggtgtg aagagtgtga
    18841 cgatgattta cagcaataca agggacttgc gttgttgaaa aatcatcagc ccatcacagt
    18901 agaaagcaga aaggaatttt atgaaacaac taattctagc ggaaaaacca agcgttgcga
    18961 aagacctaag caaggtgctt ggtgctaatc aaaagcacaa aaattattat gaaggaccca
    19021 aggtgatcgt tacttgggcc cttggtcatt tattaggatt gaaaatgccg gaagatctga
    19081 ataaggaatg gcagagctgg caaatggaca cgttgccaat gatccctaaa aaactaggaa
    19141 tcaaaccact tcctaaaaca ggacatcaat taaaagcaat caagcagctg gctcagcgaa
    19201 aagatgtctc agaagcagtc attgcgacag atgccggaag agaaggggaa cttgttgctc
    19261 gctggatctt agagtatgtg catttcaata aaccagtgaa gcggttatgg atctcttctc
    19321 aaacagataa agcaatcaaa actggattca agcaactaag acctgcgaaa gaatatgatg
    19381 cattgtatga atctgctctt gcccgggcaa aagcggactg gctggtagga ttgaatgtga
    19441 ccagagcctt aactgtgaag tacgcggata atttatcagc aggccgtgtg cagacaccga
    19501 cactagcatt agtgagagac caagaaaaga aaatcgagca gttccgtcca caaacgtatt
    19561 tcacgatcac gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg
    19621 ctttaaaaac acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa
    19681 ctgtcacaga agtccaagaa aaattaaaaa cggaatctgc gccattgcct tatgatttaa
    19741 cagagatcca aagagaagcc aatcaacgct atggctattc tgcaaagaaa acattaagtc
    19801 ttgtacaaag tttgtatgag acccataaga tcgttactta tccgcgtaca gacagcaaat
    19861 atctgacaaa tgacatgaaa agtacgatga aagaacgatt gcaagcagtc agcgactttg
    19921 ctcctgaggt caagcagtat ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc
    19981 aagacagtaa agtgaccgat caccatgcac ttttgccaac tgaaaacaga gcacgttatg
    20041 aaaaattaag taatgaagag cgaaaaatct atcaaatgat tgtctctcgt tttttaggac
    20101 tgtttgctca accccataaa gtcagccaaa caaaagttac ggttgaattt ggtaaagaac
    20161 gattcatttt tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg
    20221 agacggaaac agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa
    20281 aagaactatc tgcaccgcct aaaccattgt cagaagcaag cttgcttggt caaatggaaa
    20341 aatacagtct gggaacccca gctacgcgtg cagagattat cgagaaacta atcaaatctg
    20401 aactgatgga acgtacccct caaggactgc aagtatctcc taaaggtaag caattattag
    20461 tattggtaaa tccctcttta gtaacaccag atttgacaga aagctgggaa aaagaccttg
    20521 aagctattgc tgcaggaaag aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa
    20581 caaaacgctt ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat ttctcgatta
    20641 cccaaaagaa atgcccagaa tgtggggcca atctgcgcga gaaaaacaca cgtgacggca
    20701 aaatctatgt ctgcaccaat tcagattgca actaccgcag aagaaaagat ccaaaagtct
    20761 ctaatcatcg atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg
    20821 cttatttccg ttgtaaatac gatggaacga cagaaaaatt gcttgataaa aaagagcaga
    20881 agaagaaaat gaccaaacat gaagaacgac gcttgctgaa aaaatatgca caagcagaag
    20941 aaccagaaga aagcccatta gcagcagcac taaaagcagc aatgaagaag taataagata
    21001 tgaaagaggt tgtgatagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
    21061 gcttctcaaa tttttgtgaa atttcggctt atttccgaag aagctacttc tggaacacca
    21121 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
    21181 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
    21241 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
    21301 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
    21361 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
    21421 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
    21481 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
    21541 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
    21601 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
    21661 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
    21721 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
    21781 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
    21841 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
    21901 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
    21961 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
    22021 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
    22081 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
    22141 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
    22201 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
    22261 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
    22321 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
    22381 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
    22441 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
    22501 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
    22561 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
    22621 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
    22681 tagtccgaaa gctgtgcgaa cgattgggca ggcaaatcga accaatccat tgccaatcgt
    22741 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
    22801 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
    22861 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
    22921 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
    22981 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
    23041 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
    23101 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
    23161 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
    23221 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
    23281 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
    23341 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
    23401 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
    23461 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
    23521 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
    23581 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
    23641 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
    23701 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
    23761 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
    23821 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
    23881 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
    23941 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
    24001 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
    24061 aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DACCQP010000036        24022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCQP010000036 DACCQP010000000
VERSION     DACCQP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.036"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IVO87_001580"
     CDS             134..409
                     /locus_tag="IVO87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7449773.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IVO87_001581"
     CDS             415..1182
                     /locus_tag="IVO87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7449774.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IVO87_001582"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IVO87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7449775.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILRIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IVO87_001583"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IVO87_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7449776.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IVO87_001584"
     CDS             5326..5628
                     /locus_tag="IVO87_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7449777.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IVO87_001585"
     CDS             5631..6794
                     /locus_tag="IVO87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7449778.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IVO87_001586"
     CDS             6820..10248
                     /locus_tag="IVO87_001586"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7449779.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IVO87_001587"
     CDS             10269..11393
                     /locus_tag="IVO87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449780.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEIMSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IVO87_001588"
     CDS             11383..11820
                     /locus_tag="IVO87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7449781.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IVO87_001589"
     CDS             11804..12142
                     /locus_tag="IVO87_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7449782.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IVO87_001590"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IVO87_001590"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7449783.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IVO87_001591"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IVO87_001591"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7449784.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IVO87_001592"
     CDS             13299..14342
                     /locus_tag="IVO87_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7449785.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IVO87_001593"
     CDS             14501..15229
                     /locus_tag="IVO87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7449786.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IVO87_001594"
     CDS             15345..15590
                     /locus_tag="IVO87_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7449787.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IVO87_001595"
     CDS             15587..15805
                     /locus_tag="IVO87_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7449788.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IVO87_001596"
     CDS             15941..16480
                     /locus_tag="IVO87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7449789.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IVO87_001597"
     CDS             16473..18746
                     /locus_tag="IVO87_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7449790.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IVO87_001598"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IVO87_001598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7449791.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAVQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IVO87_001599"
     CDS             complement(19919..20173)
                     /locus_tag="IVO87_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7449792.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IVO87_001600"
     CDS             complement(20573..20842)
                     /locus_tag="IVO87_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449793.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IVO87_001601"
     CDS             complement(21312..21767)
                     /locus_tag="IVO87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7449794.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IVO87_001602"
     CDS             complement(21905..23833)
                     /locus_tag="IVO87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7449795.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8003 a         4121 c         4999 g         6899 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcgcattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat aatgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccgtgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tg
//
LOCUS       DACCQP010000037        23691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCQP010000037 DACCQP010000000
VERSION     DACCQP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.037"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..678)
                     /locus_tag="IVO87_001603"
     CDS             complement(<1..678)
                     /locus_tag="IVO87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7449796.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCN"
     gene            complement(1246..2313)
                     /locus_tag="IVO87_001604"
     CDS             complement(1246..2313)
                     /locus_tag="IVO87_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7449797.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2333..2812)
                     /locus_tag="IVO87_001605"
     CDS             complement(2333..2812)
                     /locus_tag="IVO87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP7449798.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2829..3290)
                     /locus_tag="IVO87_001606"
     CDS             complement(2829..3290)
                     /locus_tag="IVO87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7449799.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3304..4554)
                     /locus_tag="IVO87_001607"
     CDS             complement(3304..4554)
                     /locus_tag="IVO87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7449800.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4932..6383)
                     /locus_tag="IVO87_001608"
     CDS             complement(4932..6383)
                     /locus_tag="IVO87_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7449801.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6704..7468)
                     /locus_tag="IVO87_001609"
     CDS             complement(6704..7468)
                     /locus_tag="IVO87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7449802.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7496..8194)
                     /locus_tag="IVO87_001610"
     CDS             complement(7496..8194)
                     /locus_tag="IVO87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7449803.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8206..8985)
                     /locus_tag="IVO87_001611"
     CDS             complement(8206..8985)
                     /locus_tag="IVO87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7449804.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(9001..9945)
                     /locus_tag="IVO87_001612"
     CDS             complement(9001..9945)
                     /locus_tag="IVO87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7449805.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9991..10770)
                     /locus_tag="IVO87_001613"
     CDS             complement(9991..10770)
                     /locus_tag="IVO87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449806.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11150..13225)
                     /locus_tag="IVO87_001614"
     CDS             complement(11150..13225)
                     /locus_tag="IVO87_001614"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7449807.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13227..14144)
                     /gene="glyQ"
                     /locus_tag="IVO87_001615"
     CDS             complement(13227..14144)
                     /gene="glyQ"
                     /locus_tag="IVO87_001615"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7449808.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14530..15348)
                     /gene="recO"
                     /locus_tag="IVO87_001616"
     CDS             complement(14530..15348)
                     /gene="recO"
                     /locus_tag="IVO87_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7449809.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15491..16390)
                     /locus_tag="IVO87_001617"
     CDS             complement(15491..16390)
                     /locus_tag="IVO87_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7449810.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16405..16806)
                     /locus_tag="IVO87_001618"
     CDS             complement(16405..16806)
                     /locus_tag="IVO87_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7449811.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16784..17260)
                     /gene="ybeY"
                     /locus_tag="IVO87_001619"
     CDS             complement(16784..17260)
                     /gene="ybeY"
                     /locus_tag="IVO87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7449812.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17289..19481)
                     /locus_tag="IVO87_001620"
     CDS             complement(17289..19481)
                     /locus_tag="IVO87_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7449813.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19505..20476)
                     /locus_tag="IVO87_001621"
     CDS             complement(19505..20476)
                     /locus_tag="IVO87_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7449814.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21181..22560
                     /gene="brnQ"
                     /locus_tag="IVO87_001622"
     CDS             21181..22560
                     /gene="brnQ"
                     /locus_tag="IVO87_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7449815.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            22842..>23691
                     /locus_tag="IVO87_001623"
     CDS             22842..>23691
                     /locus_tag="IVO87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7449816.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYW"
BASE COUNT         6710 a         4976 c         3949 g         8056 t
ORIGIN      
        1 attacatcta taataataga tacaatcttt aataaaactt gttatttttg aatgttgcaa
       61 atatgaaata tggaatatga tatcttcaga tatataatcc ctttctgacg gaaacttgat
      121 attgtgctgt tgaattatct cagatgaata cagcgcatgc caaactgaca ttggcaatat
      181 aaaatcgact ttataactcg gctcagtgcc aatcattcca agcaacactt tttcaattat
      241 agcgttattt gtaaatatcg tactatcata tcttgctggc tttgaaataa tattattatc
      301 atcataatat tcataatatc cagcataaca tgtgtctgca ttactccttt ttataccatt
      361 taacattgct tcaatataat tagctgcaac aaaatcatca gaatcaataa aagcaacata
      421 ttttcctttt acatgttcaa gtcctgtatt tcttgccaat cccaagccag cattattttt
      481 atgaataata cgaatccttg aatcctttaa tgcatagtct tcacatattt tattagatga
      541 atccttagat ccatcatcca caattataat atctaggttc ttatatgttt gattacacac
      601 tgattccaaa caacttccta catacttttc aacattatat acaggtatga ttacactaat
      661 tagatcactc gaattcatcc ttctatcctt tcactcacgt tttctcaatt agtcaatgac
      721 ggcatcaacg attttcttaa attctctgaa aaaaatagac tctgatcttg atgatgtgaa
      781 tgacatacta tcttcttcta gaaaaattat ttcctttaaa ttatttatta aatcaataat
      841 atgatcctcg taagtatgct ttcccttgga ataatgaaaa tatacttttg tattacacaa
      901 tattgcaatt ttatttctta atatgattat ttcactcatt cattactgcc acatatacac
      961 aacttataac caccccctgc aaagtttagg gtaagttatc agataatatc ttcctcttca
     1021 tcatccagaa tacaaaatct atttatttat agaaatatca ttaatgtttt tatataatgt
     1081 accgtgcttg tactcttgct ctattacaag cactaaaaca ctactattca tttgaattct
     1141 tacacggata aaaatttaca tcgtctattt caatattttt tctctctatt ttattcactt
     1201 aataagttca ttacataatc gaaacatttt ttattttcct tgtcatcact ccttgctccc
     1261 tttttgatac agatttaaaa ttctatcaaa gaaatttcca tcgttaaatt tcgttattgc
     1321 caaatccttc gaacaacgtg aaaattttac gtatttatct ttatcattga tttttagtat
     1381 tgcagatgtc aacgctgata cgtcaccagg ttgaaacaaa tatccatttt ctccatcacg
     1441 tactgctgaa gaaatatctc ctacatctgt agcaattaca ggcattccat aagaaatcgc
     1501 ctctagaata gatataggca atccctcgtt ataggacggt aaaatcatca catttgatct
     1561 tctatagcac tcctcctttt tttctcccac aacccaacct gtaaattcaa aatacgattg
     1621 caaattatat tcttctatca attctttgag cgatatttct tcatcacctg ctccagcaat
     1681 ctcaaaaata aacttttttt ctggatattg ttctgcaatg tttttaactg cttctaccaa
     1741 atcgtgtaca ccttttcgct ttatcaaaac tcccataaac agaaatcggc acatttttga
     1801 attccacttt atggtatatt ctggaactac aatagaattt tctacaacaa cagttttaca
     1861 atttggctca atttctttaa ttatcttctt ccatttttct ccaagtacaa tcgtggaatc
     1921 agactctctt aacagcaagc gaatctgatt ttgttttttt acagaaacac tatcatacca
     1981 ttttttaaat tccgatccat gaagatggat gattacagga atatttaata atctaaacca
     2041 cttattcagc acatatttcc tactaaaact ccctctgtac gacatgtgca tatgaataat
     2101 attaggttta ctcaataaag agatacctat aattttagca aatgcataca taaaatataa
     2161 agatttaaat attgcatttc cacccttata actcggtata aaagtcatct cgatattctt
     2221 tttttcccaa ttaaaattga gcatttgtgt aattatactt gtaattccac cttttaccga
     2281 gctgtcatta ccaatcataa gtactctttg cataacgctt tactccttat atttatattt
     2341 tttctattaa tacctctatc gaattaataa tcgtttccgt gttagataca tacttgttaa
     2401 acatccgaac tcttgtttct ttaacggcat cttctaaatt ttctaattca taacaaggat
     2461 agataatatt catctcatca aattgtctaa ttaactgcag ttggtgatca tcgacatgct
     2521 caccatactt agccaatcga ggaacagcaa tgaccctttt accttttttt acggccccaa
     2581 ttatagcacc tgtacctcca tgagtaatca ccatttctgc tttttgcata gtttcagtaa
     2641 actcatccct atttagaaat gctttatagg aataatttag tggtttataa tcactataac
     2701 cactctgtgc aaatgtactt tcgattatta ctcctttttc aattaaatca tcaatagctt
     2761 ttaacaatct atcaaattga aatttttgtg aacctaatgt tataaatatc atttttttct
     2821 cctattattt aatatatccc acccaaataa atagcattag gataaaattg tagcatagtt
     2881 tcccactgaa cataaaattg atctgcaaaa tgatacatca attttcctgt ctgagtaggg
     2941 ctggttactt ttgcaaacga ttcaatataa atcagttttt ttcccatcat ttttgttatt
     3001 aagcagatag ggatcatagc taaaacacct gtacaaatca cgagatccgg tttttccttt
     3061 atatatataa taattgattt gaataaatta ataagtatag tacacaaaaa aaacttttct
     3121 tttctattta cttgtctcat gtagtacgtt ttttcgcctt ttgactcagc tgaatattct
     3181 gtctgttctg ttataacaaa actctcatac ttctccatca gtggttttaa catcattaac
     3241 tgttcataat gccccccaga ggaagccgca aaacatatct ttttgttcat attaacctct
     3301 ctatcagtca cgctgaaaaa tatctctagt gtaaactttt tctttaatat catccaagca
     3361 ggtatcatat cgattggcaa taatagaatc acacatcgcc ttaaactgct ctaaatcgtt
     3421 gataacctta cttccgaaaa aagtctctcc atctttcagt gcaggctcat aaatcaccac
     3481 agtcgcacct ttagctttta cgcgcttcat aactccctgg attgaactct gacgaaaatt
     3541 atcactgttg ctcttcatgg tcagccggta tataccgatt aaaactggat tttccctgtt
     3601 gacatcatac tgattatatt cagcgtagtt gtaatagccc gccatctgaa gaacacgatc
     3661 tgcgatgaaa tccttacggg tacgattaga ttcaacgata gcctcaatca aatttccagg
     3721 aacatctgca tagtttgcca acaactgctt ggtatctttt ggcaagcaat aacctccata
     3781 tccaaaggat ggattgttgt agtgagttcc aatacgagga tcgagacaaa caccattgat
     3841 tatctgctga gtattcagcc ccttcatttc agcataagtg tctaattcat tgaaatagct
     3901 gactcgcagt gctaaatagg tatttgcaaa cagctttact gcttcggctt cagtgaatcc
     3961 cataaaaaga gtatccacgt tctccttaat tgcacttttc tgcagaagtg ttgcaaaaac
     4021 atgagcagca tttaacaagc gctcatcagt catatcagta ccaacaataa tacgagatgg
     4081 atacagatta tcatataaag ccttgctctc gcgcagaaac tctggactaa aaataatgtt
     4141 cttactcccc gttgtctcac ggactgattc agtgtagcca acaggaattg tagacttgat
     4201 gaccatgatg gtatcaggat tcacgtccat cacaacctca ataactgact caacagcaga
     4261 agtatcgaaa tgctgagtac gactgtcata gttagtcggt gtagcaataa caacgaaatc
     4321 tgcggattta taagcgctcc ttgcatccaa agtagcagtt aagtcaagtt ctttttctgt
     4381 aagatacttt tcaatatatt catcctgaat tggagacttc cgtttattaa tcagttcaat
     4441 cttttcaggg ataatgtcta ccgcggttac ctggtgatgc tgcgccaata gtgtggcgat
     4501 actcaaacca acataaccgg tgccggctac tgcgatgttg tactttttct tcacaatatc
     4561 tcctctttcc tctcttaagt atggagaatg ttactcctac ggctattcat catgtcaaaa
     4621 taacacagct tagaatgttg ataaaataat tttccagaat gtgataaaat ctctttcatt
     4681 tataaaccac acatctaatt ttatctattc tccacaagac atgtagagta ttctttaata
     4741 acaagatacg accttactaa atacttcaaa aaattatcat atttaattca taaatataaa
     4801 tcttcatttt aaatgtatgc ccaaaaattt atctgtaatg cgtcatcaaa atctagattc
     4861 caaatcttta tcaattttta tccactcatt ctaccacttt agtaatattt tctatttata
     4921 acactttcgt cctacattga tccccccctc tgaagtacag tttttactgt cttaaataaa
     4981 attttaaaat ctaaccccat actccaattt tttatatatt ccgtgtcaag ttttgctact
     5041 tcttcaaaat cagtaatatc acttctacca cttacctgcc acattccagt aattcctggc
     5101 ttaatagcaa ttcttgcacg atgatgataa tcatattctt catactctga tttaaggcat
     5161 ggtcgaaatc ctataaggct catctttcct gttagaacgt tgaaaaattg agggaattca
     5221 tctaaagacg tggatctaat aaattgaccg attcctgtct tttttgttcc atctggaagt
     5281 attttatttc cgataattct tggatcaaac tccatcttaa acataagatt attgctcatc
     5341 ttattttctt tcattaattc tacttttctt tcttctgcat ccatatacat acttctaaac
     5401 ttatacatct tgaacggttt accattctga cctatacgtt cttgtgcaaa gaaaattggg
     5461 cctggtgaat taatataaat aagtggagca ataaagataa aaataattcc cgttgcaata
     5521 catccaacaa tacctgcaag aatatcaatt gttcgtttta taaatgcctg ttgagttgtc
     5581 ataacattga tacttgttgt aagtactgta tattctccta tacgaccaat agattgtttt
     5641 cccaaagcac tatctgatac ttttgcaaga ttcaaatgta cagtaaggcc catttcagag
     5701 catcgttcaa taaattcttt catacacgaa gtacttttat tcaaaataat aaagacttca
     5761 tctacccatc cctgacataa gtaatccgct gcatttttgg aatcagaaac aactgtcact
     5821 ccctcaacat cttcaccaat catgctttta tctatgataa tgacaccatt tattttatac
     5881 attttatagt tatgctcttt cacattacga ataactactt cagcaatact actagatgta
     5941 actataataa gtgatttgtt cccctcattt ttcattttat gtttcaagta atgtttccat
     6001 aagaggcgag ttatatatgt aattacaacg tataaacctc ccatcaagaa aagaacgact
     6061 ctagaataat catttccacc ctgaacacta atgagataca aacttcctaa cagcaccaac
     6121 ataatcgctt gctttacaga agaagcaaat tctatatagt accctctttt taatacacct
     6181 ctatatgctt caaacatgaa aataactaaa agatctgcca gttcaatgaa gactgccatg
     6241 ttttgataca ttggaatcac ataaggattc caatctacct ggcgtaagac atacgataga
     6301 taaaaagcaa gctggataca caataaatct aaaataataa aatcaatatg tttaacccat
     6361 ccccttgaac ttttcttgta cattcctttt tactcccctc tttttaataa aataaattgt
     6421 attacttgtt ttctctccta aatatctttc tgaaggatta ttgactcgac aaattggctt
     6481 gtggatattc ttatatatag aacatccgta agccaactta aaactcctta acaaaatttt
     6541 tttactcata ttgtagatca aaacaagtat ccaagaaatc atattttatt tcttttttct
     6601 gcttactcaa caacagctaa aagaaaaatc atcataaaca aattcaacta agccaacgat
     6661 ccccttgtga aatcaagttt tttcttgatt tacgaataaa gagctaaaat aaaccaaact
     6721 tcttcttttt tacctcttca tagactggat aactgatttc atccccgttg atcagatcct
     6781 ttgcgacttg tttcatctgt actacttttt ccttaccaaa gtcctttgca atctgctcat
     6841 agcactcttt taagtaaaag gtgcgcttag agaccccatg ggcatcagaa gcagccatct
     6901 gcacgagatt atgtttgacc atcgttttgg ctgttttctg gatgtctttg ccaaataccc
     6961 caacataact tggcgcagtg agttgggcca aacatcccat atctaggaac ggaagcaaat
     7021 gatttgggtc ttcgcgaaaa tgcacgttgc gttctggatg gacgattact ggtgtctttc
     7081 ctagttctaa caactcaaaa aaaagccgtt ctgcatataa cggcacttcc agtgtcggaa
     7141 attcgatcag aagatatgta tcatctaaat ctgtaaataa tatttcatct cggtggatgt
     7201 cttcgatgag cgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaact
     7261 gtcttttctc taattcggct tgtaacgaag cgactagaga aatgacttgt gatttttcat
     7321 tgctgtattt tccattattg tggtgtggtg tgcataaaat atgcgtgatg ccttgctgaa
     7381 tcgctttttc agccattgca atacttgctt ctaagttttc tgccccgtca tcgattcctg
     7441 gtaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt attccttaat
     7501 ttccgtagta ataaccagca tctttgctat gttctgctcc gttgtacaca acacctaaga
     7561 ctcgtgcttg taccatctcc agcagttctt ttgctttagt caaggattct ttacgtgaca
     7621 tattttcacg tacgaccaat aaagtgccat ccgtttttaa agacatgatc tgtgcatctg
     7681 ttacggctac tactggtggc atatcgaata tcaccacgtc aaataactgt cttgcttcgg
     7741 ataggatctg atccattctt ggcgagctta acaattctga tgggttgggt ggcttaggtc
     7801 cactaggcaa aatcgacaag ttctcgactg gcgttcgttg gatcacatct gctacacttc
     7861 ctgatgaact aagtgctgta cttaatccac tggcattgtt cagctggaat gtcttataga
     7921 cgacgggttt acgtaaatct gcatcgacta gtagaactcg ttgacctgat ttagcaaaaa
     7981 cgactgcaat gtttgctgca gtggtggatt tcccttctcc aggacctgat gaagtcacga
     8041 caatcgtttt gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac
     8101 gatattgttc ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actgcatgag
     8161 tttgtgctgc tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg
     8221 gcttctgcgt gatggggctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgttcc
     8281 ttttttacgg ataccagcgt tcaattcttt cgctgacatt tctggaactg ttcctaagat
     8341 tgggaatcct aatgtatctg tcacatattt ttcatctttc actgtccgat cgagtaattc
     8401 caataagaat gccagaccta cacctaccat cattcctaac attaagccag tcattaaatt
     8461 caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaaa tgatcgagat
     8521 tttatcaaca ttcatcacat cttttgcgtt ctcttggaaa acttgtgaag ttgtgttagc
     8581 gatctgttga gcagccactg cattcgtact tattgcctga atcgagaaca tttgtgaatt
     8641 ttctgcctga ttaactgaga tggcatcttt gatttgttca gcagtcattt tcacattgtc
     8701 ttctgtttct aaacgatctt tgacttcatt gagcactaaa tcactgacga tcatatcttt
     8761 atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgcag tgtctgattg
     8821 tggcagtgtc acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt
     8881 tacgattcct gagatggcca agccagcaaa cattgagatg atgattatta ccaagtgttt
     8941 gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc
     9001 ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg
     9061 atcactgact ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc
     9121 ttgcttaatc gtaccaaatg cctcccgata atctaaagca atttttttca tttcatcaaa
     9181 gttcatgtct gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac
     9241 accgtttaga ctcaatactt ttttcgctac gccttctacg atctttcttt ggcgcccttg
     9301 tctaccgtaa tcaccatttg ggtcttcata acgcatacga gaataagata atgcctgatc
     9361 cccgttcaat gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc
     9421 attattgact tcgataccac caactgcatc aacgagttcc tttagtcctt tcatattgat
     9481 agagacatag tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc
     9541 agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc
     9601 gacatacgta tctcgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt
     9661 ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat
     9721 ccccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc
     9781 tccttgggtt cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata
     9841 taattttgct cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat
     9901 caaaaatatt tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg
     9961 ctaaccttgc ttgaatcaac gttttaactg tcaatagctt atattgctat agcccatttg
    10021 ctgagcaaca gtttgtgctt ttgttgcgta tgcggctctt attttttcat tccggatctg
    10081 ctctatttga ctgagcgccg tcaaatattg atcgtaagta gcggtagctg ttacttgacc
    10141 gtctttcagc atcgtatcga atagctgatc tagttgcgtg acttgtgcaa tttgggtatc
    10201 agcgaatcct agatattgca taacgactgt tttccaagca ctatccttaa aaaagctcat
    10261 attttcacgt acatgggcga catctgcact tgccaacttt tctttgatga tgacatcatc
    10321 cacagcttgc tgccagtttg atacgttttt agtaaataat ttattgacag ccgtctggat
    10381 cttcagtttt gattctgcat cctctaactg cgttaagact gctttctttt cttcttggat
    10441 cgcagcagct tctttaggta attctgattc cttgatgtca taatcatctg ccgaaagttt
    10501 gatagaagat agtttatgtg acaaatttga aactgtttct tctgttattt caggatttag
    10561 gaaaattttt tcttttgtac tgtacagctt atctacttct tttgataaag aggtcacggt
    10621 gtctttttga ttggtgatgg cagattctgc gtaattgatc cgttgatgat attgccgctg
    10681 attattgaga tggtatccaa acaagcaaat cacaatagcg cctgtgccta ccgccacagt
    10741 agccaataac ttgacgaacg gcttttccat agattcactc ctaaatattg atgatagata
    10801 agtaaagatg tgattcattc acatcagaat gagcgctttt tattttaaaa gcataattaa
    10861 actaatatat tcatagaaat atttataaac aggttttccc tatataatca taccacatac
    10921 agtttttttg actagggaaa ttcctaattt aaaagtaaat aaaacagtaa caagattata
    10981 attaatcgtt ttcattttta tttcctcact tttcatgagc gttttgaaaa ttcgaacaaa
    11041 gaataaaata acaaaaatta ttaaaataga attaataaca gctatttata acaaaaaaaa
    11101 cagagacaca gcacgtttcg tgcatgctct ctgtttttca aacaaaagac tattttgtga
    11161 tgatttgatc caagcttgct aatgacaaag ccatggcagc tatttgactt agttgtgtta
    11221 gtcggttctt acgtactgct ggttcatcag ccatgaccat cgtttgatca aaataatttt
    11281 cgatcagtgg acgaagagcg acaagctggt cgtagttttc gtggatcgtt tggtcagcaa
    11341 aacttgcttt cattgcttct actgctttgt gtaattcttt ctctgcgtcg ttttcgaaca
    11401 gctcagggtc tacttctgat tgtttttcaa gctgcgcttt tttcgctaag ttcacgacac
    11461 gtgtcaaagc ttccattgaa ggtttgaagt cttcatctgc aagatgttgt ttgaacaatt
    11521 gtgctgctgc aaatactttt gtcagatctt tttgttccgc attcaaaaca gcatcgatga
    11581 catcatgacg aatattcttc gttgtcaata attggcgcag acggcctttg ataaagtcga
    11641 tcacttgttg ttgcccttct gataaaccaa tgccgaaacg atccgtatct ttactgataa
    11701 catcgctaat cgtttcttgc agaacagaca aaggaaaggc ccattttttc gattcgatga
    11761 tccgaacgac accgtatgct tggcgacgta aggcataagg atcgtttgac ccgcttggaa
    11821 tcatcccaac tgcaaagaaa gaaaagatgc tatctaattt atctgcaatc gctaaaacag
    11881 aacctactgt agactgaggt agctctcctt cactggatat aggcatgtaa tgctcacgaa
    11941 tcgctgtcgc tacagcaggt ttctcgcctt tcagaagagc atatttttcg cccatgatcc
    12001 cttgtagttc aggaaattcc cctaccatgt tggttactag atcaaattta tagatttcag
    12061 cagctcgttg caaatccatc aattcttcct cagatagtcc tacttgttcg ccaatgatac
    12121 gggcaatgac ttgtacccgt tgcattttct catagatact accgattttt tcatggaaag
    12181 taacagtttt tagtttttca acgtattggt cgattgaagc tttcttgtct tcattataaa
    12241 agaattccgc atcttctaaa cgagccgtca atactttttg attgcctcga accacatctt
    12301 ccaattttac ccgatcacca ttacgagcag aaataaaatg aggaagcagc ataccttgat
    12361 cattccgtac ctcgaaataa cgttgatgtt ctttcataga agtgactagt acttcttcag
    12421 gaacagaaag atatttttct tcgaagtcgc cagaaaagac agttggatat tcgactaaat
    12481 tgttgacttc ttctagtaaa tcttcgtcta gatcaataac ccagtgattt ttttctgcta
    12541 attcgtttgc ttgtgtaacg atcatttctt gtcttttttg cggagacgca atgacgaatt
    12601 gttcattcaa tttttcttca tattcttttg catgcgcaaa tgtcacatct tcgcctaaga
    12661 aacgatgacc tcgagaggct ctgcctgttt tgacatccaa gatttcgaat gggattactt
    12721 cgtcatctaa caatgccacg atccagtgaa tagggcggat atattcaaaa tcgtattctg
    12781 cccaatgcat cgttacaggg aaagtcaaac ttttgatcac ccctgctaag cctgtcaata
    12841 cttcttttgc ttccttacca tgagtgtatt tcgttacata gacatattcc acacctttta
    12901 attctctaaa agtgatagcg tctgtcgtca gaccttgtcc gcggacaaat ccttccgctg
    12961 ctttcgtcca gtttccatct gcgtccaaag caattttttt ggctggtcct ttgacttctt
    13021 cttcactgtc cgcttgtcgt tctggaatat ttgttacttt gattgctaaa cggcgcggtg
    13081 tggaaaatgt ttcgatcgta tcataagtca attggttttc atctaaaaat tttgctgttt
    13141 tttcttctaa ttgtttcatg cttggtgtga caacatgggc aggcatttct tccagcccga
    13201 tttcaagtaa taagtctttt gccatcttat tcagcctcct tcacggtttc tggatttgct
    13261 gtgtttttat tcaacaaagg gaatcctaat ttttcgcgtt ctgccacaaa gattttggct
    13321 acagcacgag ccatgttgcg gatgcgagaa agataaccgg cacgctctgt tacggatact
    13381 gcaccgcggg cgtctaataa gttgaatgta tggctacatt tcaagatata atcatacgcc
    13441 ggatgaacaa gattttcatc gatacagcgt ttagcttctt tttcaaaacg atcaaagttg
    13501 cttagtagca agtcttggtc gcttacttca aaagaatatt tggaatgttc atattctggt
    13561 tgtttgaaga tctcgccata tttcacgcca tcagcccatt ctaaatcata gacactttca
    13621 acttcttgga tataagaagc aagacgttct aatccataag tgatctcaga agttactggt
    13681 ttacaaggca gtccaccaac ttgctggaag tacgtgaatt gtgtgatttc cattccgtca
    13741 agccatactt cccaacccac accggcacag cccatggatg ggttctccca gttgtcttca
    13801 acaaaacgga tgtcgtgttc taatggatcg attcctaaaa ggcgcaagct ttctaaataa
    13861 agctcttgaa tattctcagg tgaaggtttc atcaccactt ggaattgatg atgctgatac
    13921 aaacggtttg ggttttcacc ataacgtcca tcagctggtc ggcgagatgg ctctacgtaa
    13981 gcagcattcc atggttctgg tccgattgcc cgtaaaaagg tatatgggct catcgtacct
    14041 gctccttttt ctgtgtcata agcttgcatc agcatgcagc cgtttgatga ccaaaatttt
    14101 tgtaatgtta aaatcatatc ttggacggtc aattttttac tcatctgttt tgcccctttc
    14161 aattttaaag cgttgctgtc aaataagaat acaggaaaga aaacgacaaa aaatccctat
    14221 gtctgattac agacataggg acgaataatt cgcggttcca ccctacttcc gaataaagat
    14281 tcggcagctt catttatcgt actcgcaagt gcctcttcaa aaatcgtttg ggtccggctc
    14341 ccactctccc gaactcgcta aaccacgtgt tttttactga tcttggtcaa tgtacgtatt
    14401 cattttgcag ttttcttgtt tctataatag atcctatcac tgaacgatct atttaatcta
    14461 tggttaataa tagacggaac tccctgtgtt tgtcaaatgg ttttgatttt ttgttgtatg
    14521 ttttatttac tattcttctt tttcaggtgt ctctttttct ttttcagaca tgtctttctc
    14581 tgacgtcttt aatacgtgtg cccaagactt catttgattg atgaactttt tgcttttcaa
    14641 atggataccg acatattcat catacagctg gtcgatgagt tcacggattg cttgtttggt
    14701 ttcttctttc agatggattc cactaatttt atcataagaa atagctgaaa acatccgaac
    14761 gaaataaaca gctcgaggat ctgcatgata acggtgatcg tccatcgacc aatgtctttc
    14821 gcatatcaca ccaccatatt tggaagaata gtcaaatttt ccttttgttt ccccgcatac
    14881 gacgcagtgt gtccagattg gtgcaattcc gaagcgttgc agtatttgga cttcaaaaat
    14941 attcgtgatg atttctgcat cgtctccttg gtccatccgt tgcagtgctt gttgcgtaaa
    15001 ctggaacaaa tgcgggtcgt actgatgatc ttcgattgcc gcatctacta gattcaataa
    15061 atatgtgcca tagccgctca aaaagatgtc ttcttggatg tgtcgaaacg catggacatc
    15121 tttgacactg cttaaaaatg acaaaccttc acttcgaaaa ttcccgatat aaactgcttc
    15181 agtaaaaggg agcaaagccg gagctaaggg ctgattttgc cgatggatct ctttgatata
    15241 aaacatcatt ttgccagaag attctgtaaa gattttgact aatttatctt tttctttgta
    15301 attacggcta gataaaatca agccttttga ttctgcaagc tggctcatat tctccctcct
    15361 aaaaaataaa tctggaaaag cagattctgc cgatccagat catttatgtt gcgatcttcc
    15421 gcagcttttc tcaacacgcg gatttttatt cagtttattt ctatacctaa tttacaagaa
    15481 gctcagaata tcagtattcg tcttttcggt agccgaagtc ttgcagatag acttttttgt
    15541 ctcgccagtc tttttgaact ttgacccaaa gttctagaaa gactttgtct cctaaaagat
    15601 tttcgatatc ttttcttgct ttcgttccta cttgcttcag catttttccg cctttgccga
    15661 taatgatccc tttttggcta tctctttcga caataatcgt agcctggata tggattttat
    15721 cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac agaatgaggc acttcgtctc
    15781 gtgttaaaaa caagactttc tcacggatca attcagaaac gatgaagtat tcaggatgat
    15841 cagtcacttg atcatctgga aaatattgtg ggccctctgg catttcactg actaatacat
    15901 ccatcaaacg ttcgacgttg tttccttctg tagcagagat tggaatcact tgtgcaaaag
    15961 gcatctgcgt tgaataatct tcgatgattc ccaacaactc gtctggatgg attttgtcga
    16021 ttttattgat aatcagataa acaggagaat gcacgttttt tagacgttca ataataaaat
    16081 cgtctccccg accgcgtttt tgatcagcac taatcataaa taagaccgcg tccacttctt
    16141 tcaacgcact gtaagctgct tctaccataa aatctcctaa acgatgcttt ggtttatgga
    16201 ttcctggtgt atcaataaaa atcaactgag cttctggtgt agtgtatact ccttggattt
    16261 tatttctagt tgtctgtgcc ttgtcactca tgatggcaat tttttgaccc acgattcgat
    16321 tcaataatgt ggatttccct acatttgggc ggccgacgat tgcgacaaac cctgatttat
    16381 gttctggcat atatgttctc ctttttaatt aaacaacaaa tgatatattt tagggacaaa
    16441 taaaatcaac ccaacgatga cggtaaaaca agcggtcact aaaactgctc cagctgccat
    16501 atctttgatt tttttcccaa tcggatgaaa atgaaaatcc gtaaacatgt ccacgacatt
    16561 ttcaaaaaca gtattgatga tctctactat ccatacgaga aaaacagcta aaagcagcca
    16621 taaccattct atccgatcaa gttggaaaat cacgcccata aacaaagcga ggattccgaa
    16681 cgcaacatgt ttcctcatgt tgcgttcttc ctcaaaaaca gttttgactc cttggacggc
    16741 aaattccacg gacatcagga aatgtttgtt tttctctacg tttttatctt ttaagtccat
    16801 aggcatctaa gatctctttc tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat
    16861 ggtcataccc gttgatatgc aagaatccat gaaccgctaa aaagccaagt tcccgatcat
    16921 acgaatggcc gtaagcttct gcttgctctg cagcacgttc agtagagatc atgatgtctc
    16981 ccagtacacg aggcaatgga ttctctccat cttcaaaaat cactgggatt tcgtcttcgc
    17041 cctcttcttc caaggcaaaa ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac
    17101 gattgattac ttggattgct gcattgtcca tgaaagtaac agacatttct gtttcttctg
    17161 gcaactctaa atatgatgct gcaaattgca gcaagtcgtc gatttctttc attttctctt
    17221 ctgagacttg gtttgtttca tccatgaagg taatatccat tatacatcgc ttctttctat
    17281 tcttcttgct attcttcttg ttcttctttt atcttctcaa cttccgattt cattttagga
    17341 tatttgatcc gagaatggaa ggtactgctt aaaccgctga tcaatgattt ttctacttta
    17401 cggatttctt ttaacgtcaa tccactgtcg tccaattgtc catcggaaat acgctcttca
    17461 ataagattat ggacaaaact ttcgattttt tcgctcgttg gatggtccat tgcccgtact
    17521 gccgcttcac aactgtcagc tatattgacg attcctgctt cacggctttg aggttttggt
    17581 ccaggataac ggaattgctc ttccgtaact tccgggttgc gttcctttgc cttaacgtag
    17641 aaataacgca tcagcgtggt tccatgatgc tgtctacaaa tatcgatcac catttgcggc
    17701 atattataat cttccaatat tttggcacca tctgtcacat gtccaaagat aatatgttta
    17761 ctgtcttctg gcaataagaa attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg
    17821 aaaaagctgg cgtgtttgat cttgccgata tcatgataat agcaagctac tcgagttagt
    17881 aaagaacgtc caccgatttc tgctacagca ttagcactta gattggctac catcatgcta
    17941 tggtgatacg tacctggtgc ttcttccagc aactgtttca ataaaggatg attgggattg
    18001 ctgagttcat ttaatacgat cacactatca tcattgacca tcaattcaat ataggggtgc
    18061 agacctatcg tcagcaagaa tgaaaacagt gcacctgtga atccgcagac tagcgttaac
    18121 caagtcgttg catccccaaa gctcattcct tggtagataa tcaaaatgac atccatcatc
    18181 acaggaaaaa taatgaccca catagcagcg gataaccctt gttctgagat ccgtttgcgt
    18241 ctgacgatcg ttgccatgag accagaaaat acataggaca tcaaaataac tgtcagaaag
    18301 ttcgtgccaa tcgaatcata aaaaatgaaa agtgcggata ctgcttgaaa aataccagcc
    18361 atgatccctg ctcttcgatt caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg
    18421 taaaacagcg gaatataagc agcttgttct gtttggaaga gttggaaaaa tttcattagt
    18481 acgacgctta ctgacattgc cgtcacgtag aaaagtacgt attttgttct ttctcctgca
    18541 ttttggaatt gaagtgaatt ataaatcagt acaccgattt gaagcaaaac agctaaaacc
    18601 atggcaatga gtgggaaaat agaagtcgtt tgattcgtca atcctagtaa ttccaatttt
    18661 ttcatcgctg aagcatcaat ttgatttcct tcgcgaacaa taatttctcc ttggaagatc
    18721 atcactggtt gtacagcttc tcgtgcagat tgttttaatt cttctgtctt tttctcattc
    18781 aatacatcat taactacgat gccttgatcc actaagtagc gaatcatttg ttgctgctct
    18841 gttgtgacat tcaaatattg aatctgatct tctgcttctt gtttaaaagc ttccaattcg
    18901 ctttcacgaa cttgtttact catctgttcg tcaatgagct tcaaactttc atccctaact
    18961 gtgtgaattt ccgtttcagt catttcaaat atcgtcgaat aaaaattgtt cggcagtttt
    19021 tggtaaaacg caacgttttc tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc
    19081 cgctcttctg tagtaggttt cgggatattt tcatcttcct tcgccttatc taccttgcca
    19141 ttataagatt tgttgatggt atcattcgtc ttatcgatca attcgaataa ttgcttgatc
    19201 cgattgttct ggtcatctgc cagatctttt tgataagtat attctggagt cactgcttct
    19261 gctgccagtt ttcttctttg ttcggtttct tccgtgttct caatcgtttt attcgcacgg
    19321 atactctctt ctgctacttg gccttctctg aaatcaatgg ccttttgttt gacactacta
    19381 aatgtaacgc cgcagacaat gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt
    19441 ccgagttttt cttgtagttt ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca
    19501 aagcttattc ttgatgaaga tccgcctttt cataagcttt gatgatttcg gcaactacag
    19561 ggtgccgaac aacgtcactt gcttcaaaat taacaaaatc gatttttttg atttgcttta
    19621 atgttcgttc tgcatgaaca agaccacttg tcgtcccacg aggcaagtct atctgacttg
    19681 tatccccatt gacaatcatt tttgattgat aaccgagacg ggtcaagaac attttcatct
    19741 gagcgactgt cgtgttttgt gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc
    19801 cccgcatgta agctaaaggt gcgatttcga tcacgcctcg ttccatcaat cggttcgtat
    19861 gatccaatcc aaaaatttgg tataaagcat cataaacagg ccgcagataa ggatccactt
    19921 tttctttcag atccccgggt agaaagccta ggttttcccc tgcctccaca gcgggccgcg
    19981 tcaaaatgat tttttgcact tcgccttttt ttagggcagc aattgctaat actacggcaa
    20041 gaaacgtttt ccctgttccg gctggcccta caccaaatac gatgtcatgt ttggctaccg
    20101 attcaacgta tttcttttga ccgctgtttt ttacccgtat aggttttcca ttacggtctt
    20161 tgacgatctc ttcctcatac atctcaacaa aataatctaa tgtacctttt tgccccattt
    20221 tcaatgccgt cacaacatct ggcgtactta cgtggattcc tcgtttgatc aattcctgta
    20281 atgtccgaat gactgaggca gccaaggaaa tctgggattg ctcaccaatg atctgaatga
    20341 tttctccacg tgtatggatg gttgtttccg ttgtttcctc gatcagtttt atatgcttgt
    20401 cgtgtgcacc taaaagcatg ctgacatcat cagcagctgt caatctaatc tctaaagaac
    20461 tagactcttc cgtcaaaaat agacatcccc tttttttcga ttcattcttt cctattttac
    20521 catactcaga agaaactata tagcaaataa gaaaaggttg taaaagagtc gcttaaccat
    20581 acgtaccaag aaacgttaaa aaatagccgt ttccattcgc taaaggtctc gttttgataa
    20641 aaagaagctg agaatgtaag ttatatgaaa gtggagaaca aaatatttcg tttcattcaa
    20701 caagtattct ctaggtattt ttcctgtttc aaaaaaagaa aacaaaatag ttaaaatatt
    20761 cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca aataaacata cagcaaagcg
    20821 tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt gagtcatgta agtgagaaat
    20881 gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg tagatcattg
    20941 tggctgcctc cattatcagg ctagcggata actttatcaa aaagcgtccg tgaatgaaga
    21001 agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt ttcccaagtg
    21061 gaaaagggct tttttttatt ttaaggtggt ggttttcatg gaatctgata ttagtggcat
    21121 tactaattgc tgcaacgttt atatcgtcaa tcaaaatgat aattattgga ggaaaaattt
    21181 atggaagaaa caaaaactta ttcatggaaa caactattga tggtcagttc acttatcttc
    21241 gggatgttct tcgggtcagg taatctaatc tttcctgtcc acttaggtca aatggctggc
    21301 gcaaactggg tcagtgctgg cgtaggattt gctatctcag gtgctctttt cccattacta
    21361 gctatcttag ctgtagtagt gacaaaaagt gatggtctgt atgacttagc taaacctgtc
    21421 ggaaaatggt atgctgcctt gttcttaatc cttgttcatt tgacgatcgg accatttttc
    21481 ggtacaccac ggacagctgc tactgctttt gaaatggctg ctaaaccatt tttgcctgaa
    21541 aaatataatt cattaggtat gtttttattc tctgctcttt tctttttact tgcttatttc
    21601 cttactgtcc atccaacacg gttggtcaaa tacgtcggaa agtatctaaa ctccttgttt
    21661 cttgcattat tagcaatcat ttttgtcgtt tctttattcc atccaatggg aaatctaaat
    21721 caatctgctg cagatactta tcaaacaaac gctttaacaa atggtgtcct agaaggttat
    21781 aacacggttg atgcagtagc tttgcttgcc ttaagtgtca cttttgtcca tgcagtccgt
    21841 gaccttggtt tcaaagataa aaaagtcaca gaattaacgg caaaagctgg tagcctgagc
    21901 gttgcgctag aagtcatgat ctattttgga cttgttcttc tcggtgcgat gagtttgaat
    21961 aagatgtctt tatcagaaaa tggcggaact gcactttctc agatcactaa tttttattta
    22021 ggtaacttcg gcgctagctt cttaggagtg atggtcacat taggtgtctt cactaccgct
    22081 atgggactcg ttgtttcttt tgcgcaagat ttccataaac tgttccctaa agtcagttat
    22141 atgacatggc tgcggttaac gactttcgtc tcttttgtcg ttgcaaatgc tggtttagac
    22201 aatatcatcc aatggtcttt accagtattg atgcttttgt atccattgtc gcttgcgttg
    22261 atcttgcttt ccttaacagc aaaatttttc caaaagacac catttgtcta tcaagtaacg
    22321 atgttgtttg ctgctgttcc tgccgtattg gatatgctgg caaattcgcc tgctttagtg
    22381 agtcaacaaa gagttgtagc aagcatgctc gaattttatc atcatcacgt tccttttgct
    22441 gcattaggac ttggctggat ggtcccaacg ttacttggtt acgctggcag cctactgttt
    22501 tattacgcct atcgcctatc agggtataaa caagaagcaa atgaactacc agaagaataa
    22561 ccaaatcaaa cacaatttaa cataagaaat attaaacagg aaaaaaccag ctagatgaaa
    22621 gaatcctatt ctttcgtcta gctatttttt agttctcttt tttacgcgca caaaaaaact
    22681 gcggacaatg attgtccgca gtttaaatgg gctctataat atctagtgtt ggtgacgcct
    22741 tctgttgcca atactttttc ttttttctaa taaacgcaaa cagacacctt gaaatccttt
    22801 agaataaagt cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat tcagagttta
    22861 ccaaagagtt actagatatt ctagacctaa atcttatctt tcatgaagat gcctttcgca
    22921 aagagcgaat caatgacgaa acatgttttg tttttgatgg cacattgacc tatcaaccag
    22981 aggaatgctt ccattgtcac tatcaaaata agcaaacgat cattaaatgg ggctggaaaa
    23041 aagtatccat cttattgaat gatgtcagta actacaagac cattctgcgg attaacaaac
    23101 agcgcttcaa atgtaaacac tgtggaaaga cttttttagc agaagattca gttagtgatc
    23161 gtcgctgctc cattgctcgc agagtcaaac aagccattct tgagctatta agcgaacctc
    23221 tttctatgtc tttgatcgca cgtatgaagc acatttctcc gaccactgtc attcgaatac
    23281 ttcgcagcct tcggcctaaa accgtttctt tgaaccagcc gttaccagaa gtggtgtgtt
    23341 tcgatgaatt caaatcggtg aaaaacgtct cgggagccat gagctttgtc atgatggacg
    23401 gcaagaccca tcaactgatt gatattgtgg aaaatcggca actaaatcct ttacgcgact
    23461 atttcttgcg ctaccctaga aaagtccgtg aacaagtacg tctagtggtt tcagatttct
    23521 atacacccta tcgcacattg gtgaaagagt gctttccgaa tgcccgaatc gtcgccgatc
    23581 gctttcatat tagccaacac atcggtcggg cctttaccaa ccaccgaatt caagtaatga
    23641 agacctttaa aaaaagggat cgtcggtcga agcacttaaa gaaatactgg a
//
LOCUS       DACCQP010000038        23016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCQP010000038 DACCQP010000000
VERSION     DACCQP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.038"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(160..444)
                     /locus_tag="IVO87_001624"
     CDS             complement(160..444)
                     /locus_tag="IVO87_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449817.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="IVO87_001625"
     CDS             complement(462..1352)
                     /locus_tag="IVO87_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7449818.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="IVO87_001626"
     CDS             complement(1372..1590)
                     /locus_tag="IVO87_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449819.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="IVO87_001627"
     CDS             complement(1560..3260)
                     /locus_tag="IVO87_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7449820.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="IVO87_001628"
     CDS             3527..4300
                     /locus_tag="IVO87_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449821.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IVO87_001629"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IVO87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7449822.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="IVO87_001630"
     CDS             complement(5124..6095)
                     /locus_tag="IVO87_001630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7449823.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IVO87_001631"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IVO87_001631"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7449824.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IVO87_001632"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IVO87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7449825.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="IVO87_001633"
     CDS             complement(9050..9874)
                     /locus_tag="IVO87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7449826.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="IVO87_001634"
     CDS             complement(9847..10527)
                     /locus_tag="IVO87_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7449827.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="IVO87_001635"
     CDS             complement(10550..11488)
                     /locus_tag="IVO87_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7449828.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /locus_tag="IVO87_001636"
     CDS             complement(11733..12584)
                     /locus_tag="IVO87_001636"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7449829.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IVO87_001637"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IVO87_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7449830.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="IVO87_001638"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="IVO87_001638"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7449831.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IVO87_001639"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IVO87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7449832.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IVO87_001640"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IVO87_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7449833.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="IVO87_001641"
     CDS             complement(19462..20703)
                     /locus_tag="IVO87_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7449834.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IVO87_001642"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IVO87_001642"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7449835.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22614)
                     /locus_tag="IVO87_001643"
     CDS             complement(21730..22614)
                     /locus_tag="IVO87_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7449836.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         6550 a         5014 c         3983 g         7469 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaa
//
LOCUS       DACCQP010000039        21832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCQP010000039 DACCQP010000000
VERSION     DACCQP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.039"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVO87_001644"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVO87_001644"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7449837.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVO87_001645"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVO87_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7449838.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVO87_001646"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVO87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7449839.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVO87_001647"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVO87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7449840.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVO87_001648"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVO87_001648"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7449841.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVO87_001649"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVO87_001649"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7449842.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVO87_001650"
     CDS             complement(8053..8523)
                     /locus_tag="IVO87_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7449843.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVO87_001651"
     CDS             complement(8569..8994)
                     /locus_tag="IVO87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7449844.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVO87_001652"
     CDS             complement(9061..9330)
                     /locus_tag="IVO87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7449845.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVO87_001653"
     CDS             complement(9341..10936)
                     /locus_tag="IVO87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7449846.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVO87_001654"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVO87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7449847.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVO87_001655"
     CDS             complement(14489..15049)
                     /locus_tag="IVO87_001655"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7449848.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVO87_001656"
     CDS             15402..16376
                     /locus_tag="IVO87_001656"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7449849.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVO87_001657"
     CDS             16546..17946
                     /locus_tag="IVO87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7449850.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVO87_001658"
     CDS             complement(18024..18746)
                     /locus_tag="IVO87_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7449851.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVO87_001659"
     CDS             complement(18825..19331)
                     /locus_tag="IVO87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7449852.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVO87_001660"
     CDS             19454..21253
                     /locus_tag="IVO87_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7449853.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KYAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21832)
                     /locus_tag="IVO87_001661"
     CDS             complement(21321..>21832)
                     /locus_tag="IVO87_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7449854.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4789 c         3964 g         7030 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaata
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tg
//
LOCUS       DACCQP010000040        21007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCQP010000040 DACCQP010000000
VERSION     DACCQP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.040"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IVO87_001662"
     CDS             168..362
                     /locus_tag="IVO87_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449855.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVO87_001663"
     CDS             631..930
                     /locus_tag="IVO87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449856.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVO87_001664"
     CDS             complement(1027..1461)
                     /locus_tag="IVO87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7449857.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVO87_001665"
     CDS             complement(1693..2403)
                     /locus_tag="IVO87_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7449858.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVO87_001666"
     CDS             complement(2393..3253)
                     /locus_tag="IVO87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7449859.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVO87_001667"
     CDS             complement(3258..3896)
                     /locus_tag="IVO87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7449860.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVO87_001668"
     CDS             complement(3911..4210)
                     /locus_tag="IVO87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7449861.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVO87_001669"
     CDS             complement(4253..5737)
                     /locus_tag="IVO87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7449862.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVO87_001670"
     CDS             complement(5757..6218)
                     /locus_tag="IVO87_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7449863.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO87_001671"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO87_001671"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7449864.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVO87_001672"
     CDS             7521..8291
                     /locus_tag="IVO87_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7449865.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVO87_001673"
     CDS             complement(8564..9391)
                     /locus_tag="IVO87_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449866.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNPMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVO87_001674"
     CDS             9959..11194
                     /locus_tag="IVO87_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7449867.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVO87_001675"
     CDS             complement(12193..12453)
                     /locus_tag="IVO87_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7449868.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVO87_001676"
     CDS             complement(12480..13781)
                     /locus_tag="IVO87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7449869.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVO87_001677"
     CDS             complement(14073..14531)
                     /locus_tag="IVO87_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7449870.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVO87_001678"
     CDS             complement(14544..15869)
                     /locus_tag="IVO87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7449871.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVO87_001679"
     CDS             complement(15886..17298)
                     /locus_tag="IVO87_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7449872.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVO87_001680"
     CDS             complement(17315..18079)
                     /locus_tag="IVO87_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7449873.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVO87_001681"
     CDS             complement(18183..19058)
                     /locus_tag="IVO87_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7449874.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IVO87_001682"
     tRNA            complement(19313..19397)
                     /locus_tag="IVO87_001682"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IVO87_001683"
     tRNA            complement(19415..19486)
                     /locus_tag="IVO87_001683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IVO87_001684"
     tRNA            complement(19496..19568)
                     /locus_tag="IVO87_001684"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IVO87_001685"
     tRNA            complement(19630..19714)
                     /locus_tag="IVO87_001685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IVO87_001686"
     tRNA            complement(19735..19805)
                     /locus_tag="IVO87_001686"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IVO87_001687"
     tRNA            complement(19851..19922)
                     /locus_tag="IVO87_001687"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IVO87_001688"
     tRNA            complement(19934..20006)
                     /locus_tag="IVO87_001688"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IVO87_001689"
     tRNA            complement(20009..20082)
                     /locus_tag="IVO87_001689"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IVO87_001690"
     tRNA            complement(20089..20169)
                     /locus_tag="IVO87_001690"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IVO87_001691"
     tRNA            complement(20171..20246)
                     /locus_tag="IVO87_001691"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IVO87_001692"
     tRNA            complement(20282..20354)
                     /locus_tag="IVO87_001692"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IVO87_001693"
     tRNA            complement(20364..20436)
                     /locus_tag="IVO87_001693"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IVO87_001694"
     tRNA            complement(20452..20523)
                     /locus_tag="IVO87_001694"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IVO87_001695"
     tRNA            complement(20538..20627)
                     /locus_tag="IVO87_001695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="IVO87_001696"
     tRNA            complement(20667..20740)
                     /locus_tag="IVO87_001696"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IVO87_001697"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IVO87_001697"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6617 a         4409 c         3339 g         6642 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcggatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gtacttttct tttaacctat tggttaagtc ctcgatcgat tagtatcagt ccgctccata
    21001 catcact
//
LOCUS       DACCQP010000041        20916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCQP010000041 DACCQP010000000
VERSION     DACCQP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.041"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="IVO87_001698"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="IVO87_001698"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7449875.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="IVO87_001699"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="IVO87_001699"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7449876.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="IVO87_001700"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="IVO87_001700"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7449877.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3413)
                     /gene="tsaB"
                     /locus_tag="IVO87_001701"
     CDS             complement(2589..3413)
                     /gene="tsaB"
                     /locus_tag="IVO87_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7449878.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="IVO87_001702"
     CDS             complement(3502..4482)
                     /locus_tag="IVO87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7449879.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="IVO87_001703"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="IVO87_001703"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7449880.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="IVO87_001704"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="IVO87_001704"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7449881.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7239..7406
                     /locus_tag="IVO87_001705"
     CDS             7239..7406
                     /locus_tag="IVO87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7449882.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7468..9279)
                     /locus_tag="IVO87_001706"
     CDS             complement(7468..9279)
                     /locus_tag="IVO87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7449883.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9276..9641)
                     /locus_tag="IVO87_001707"
     CDS             complement(9276..9641)
                     /locus_tag="IVO87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7449884.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9804..10199)
                     /locus_tag="IVO87_001708"
     CDS             complement(9804..10199)
                     /locus_tag="IVO87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7449885.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10217..11179)
                     /locus_tag="IVO87_001709"
     CDS             complement(10217..11179)
                     /locus_tag="IVO87_001709"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7449886.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11179..11391)
                     /locus_tag="IVO87_001710"
     CDS             complement(11179..11391)
                     /locus_tag="IVO87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7449887.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11412..12110)
                     /locus_tag="IVO87_001711"
     CDS             complement(11412..12110)
                     /locus_tag="IVO87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7449888.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12130..12672)
                     /locus_tag="IVO87_001712"
     CDS             complement(12130..12672)
                     /locus_tag="IVO87_001712"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7449889.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12804..13808)
                     /locus_tag="IVO87_001713"
     CDS             complement(12804..13808)
                     /locus_tag="IVO87_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7449890.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13805..14794)
                     /locus_tag="IVO87_001714"
     CDS             complement(13805..14794)
                     /locus_tag="IVO87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7449891.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14791..15597)
                     /locus_tag="IVO87_001715"
     CDS             complement(14791..15597)
                     /locus_tag="IVO87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449892.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15763..16719
                     /locus_tag="IVO87_001716"
     CDS             15763..16719
                     /locus_tag="IVO87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7449893.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16796..17314)
                     /locus_tag="IVO87_001717"
     CDS             complement(16796..17314)
                     /locus_tag="IVO87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7449894.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="IVO87_001718"
     CDS             complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="IVO87_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7449895.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17779..18225
                     /locus_tag="IVO87_001719"
     CDS             17779..18225
                     /locus_tag="IVO87_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7449896.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="IVO87_001720"
     CDS             complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="IVO87_001720"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7449897.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(20590..>20916)
                     /locus_tag="IVO87_001721"
     CDS             complement(20590..>20916)
                     /locus_tag="IVO87_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7449898.1"
                     /translation="WGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWT
                     KYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLE
                     QLLSTV"
BASE COUNT         6227 a         4479 c         3606 g         6604 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7081 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7141 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     7201 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     7261 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     7321 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     7381 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     7441 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     7501 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     7561 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     7621 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     7681 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     7741 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     7801 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     7861 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     7921 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     7981 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8041 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8101 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     8161 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     8221 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     8281 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     8341 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     8401 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     8461 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     8521 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     8581 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     8641 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     8701 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     8761 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     8821 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     8881 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     8941 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9001 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9061 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9121 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
     9181 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
     9241 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
     9301 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
     9361 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
     9421 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
     9481 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
     9541 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
     9601 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
     9661 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
     9721 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
     9781 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
     9841 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
     9901 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
     9961 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10021 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10081 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10141 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    10201 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    10261 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    10321 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    10381 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    10441 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    10501 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    10561 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    10621 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    10681 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    10741 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    10801 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    10861 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    10921 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    10981 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11041 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11101 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    11161 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    11221 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    11281 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    11341 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    11401 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    11461 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    11521 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    11581 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    11641 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    11701 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    11761 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    11821 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    11881 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    11941 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12001 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12061 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12121 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    12181 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    12241 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    12301 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    12361 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    12421 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    12481 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    12541 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    12601 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    12661 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    12721 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    12781 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    12841 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    12901 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    12961 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13021 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13081 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13141 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    13201 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    13261 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    13321 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    13381 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    13441 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    13501 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    13561 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    13621 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    13681 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    13741 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    13801 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    13861 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    13921 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    13981 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14041 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14101 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    14161 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    14221 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    14281 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    14341 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    14401 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    14461 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    14521 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    14581 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    14641 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    14701 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    14761 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    14821 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    14881 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    14941 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15001 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15061 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15121 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    15181 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    15241 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    15301 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    15361 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    15421 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    15481 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    15541 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    15601 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    15661 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    15721 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    15781 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    15841 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    15901 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    15961 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16021 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16081 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16141 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    16201 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    16261 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    16321 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    16381 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    16441 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    16501 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    16561 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    16621 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    16681 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    16741 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    16801 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    16861 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    16921 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    16981 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17041 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17101 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    17161 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    17221 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    17281 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    17341 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    17401 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    17461 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    17521 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    17581 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    17641 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    17701 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    17761 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    17821 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    17881 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    17941 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18001 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18061 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    18121 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    18181 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    18241 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    18301 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    18361 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    18421 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    18481 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    18541 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    18601 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    18661 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    18721 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    18781 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    18841 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    18901 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    18961 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19021 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19081 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    19141 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    19201 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    19261 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    19321 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    19381 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    19441 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    19501 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    19561 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    19621 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    19681 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    19741 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    19801 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    19861 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    19921 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    19981 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20041 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    20101 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    20161 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    20221 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    20281 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    20341 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    20401 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    20461 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    20521 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    20581 gatcgtgtct cagactgtag acaaaagctg ttccaaactc gtaactcgag cttggaacag
    20641 ctttttatct acttcgtttc gaatttttga acaaaaaaat aaaggctcct tgcctttcca
    20701 tagccaggtg gctaatttct ttaaattcat tgcagcaaac actagcatcg tatgcgtcgc
    20761 gactttttcc aagcctcgat acttggtcca acgcatccca tgcttttctt ttgcatccgc
    20821 aaatattcgt tcaatcgttt gtttccgttt tttgtaaatt gatttattca gatctgttag
    20881 gcgaagatgt tccacttcat ccatcagatc tcccca
//
LOCUS       DACCQP010000042        20362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCQP010000042 DACCQP010000000
VERSION     DACCQP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.042"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IVO87_001722"
     CDS             292..1431
                     /locus_tag="IVO87_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7449899.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IVO87_001723"
     CDS             complement(1498..2073)
                     /locus_tag="IVO87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7449900.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IVO87_001724"
     CDS             complement(2078..3052)
                     /locus_tag="IVO87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7449901.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IVO87_001725"
     CDS             3335..4303
                     /locus_tag="IVO87_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7449902.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IVO87_001726"
     CDS             4416..4694
                     /locus_tag="IVO87_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7449903.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IVO87_001727"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IVO87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7449904.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IVO87_001728"
     CDS             5160..5906
                     /locus_tag="IVO87_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7449905.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IVO87_001729"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IVO87_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7449906.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IVO87_001730"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IVO87_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7449907.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IVO87_001731"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IVO87_001731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7449908.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IVO87_001732"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IVO87_001732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7449909.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IVO87_001733"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IVO87_001733"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7449910.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IVO87_001734"
     CDS             10643..11185
                     /locus_tag="IVO87_001734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7449911.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IVO87_001735"
     CDS             11208..12764
                     /locus_tag="IVO87_001735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7449912.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IVO87_001736"
     CDS             12778..13680
                     /locus_tag="IVO87_001736"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7449913.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IVO87_001737"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IVO87_001737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7449914.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IVO87_001738"
     CDS             15123..15545
                     /locus_tag="IVO87_001738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7449915.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IVO87_001739"
     CDS             16014..16244
                     /locus_tag="IVO87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7449916.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IVO87_001740"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IVO87_001740"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7449917.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IVO87_001741"
     CDS             17655..17831
                     /locus_tag="IVO87_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7449918.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IVO87_001742"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IVO87_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7449919.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IVO87_001743"
     CDS             18326..18709
                     /locus_tag="IVO87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7449920.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IVO87_001744"
     CDS             18878..19762
                     /locus_tag="IVO87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7449921.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IVO87_001745"
     CDS             19879..20112
                     /locus_tag="IVO87_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7449922.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IVO87_001746"
     tRNA            20200..20280
                     /locus_tag="IVO87_001746"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IVO87_001747"
     tRNA            20287..20358
                     /locus_tag="IVO87_001747"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DACCQP010000043        20008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCQP010000043 DACCQP010000000
VERSION     DACCQP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.043"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            314..1975
                     /locus_tag="IVO87_001748"
     CDS             314..1975
                     /locus_tag="IVO87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7449923.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2045..2392)
                     /locus_tag="IVO87_001749"
     CDS             complement(2045..2392)
                     /locus_tag="IVO87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7449924.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2476..3417
                     /locus_tag="IVO87_001750"
     CDS             2476..3417
                     /locus_tag="IVO87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449925.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3418..4461
                     /locus_tag="IVO87_001751"
     CDS             3418..4461
                     /locus_tag="IVO87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7449926.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4467..5537
                     /locus_tag="IVO87_001752"
     CDS             4467..5537
                     /locus_tag="IVO87_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449927.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5527..6480
                     /locus_tag="IVO87_001753"
     CDS             5527..6480
                     /locus_tag="IVO87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7449928.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6492..7502
                     /locus_tag="IVO87_001754"
     CDS             6492..7502
                     /locus_tag="IVO87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7449929.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7787..8608)
                     /locus_tag="IVO87_001755"
     CDS             complement(7787..8608)
                     /locus_tag="IVO87_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7449930.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8705..8983)
                     /locus_tag="IVO87_001756"
     CDS             complement(8705..8983)
                     /locus_tag="IVO87_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449931.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9251..11260
                     /gene="metG"
                     /locus_tag="IVO87_001757"
     CDS             9251..11260
                     /gene="metG"
                     /locus_tag="IVO87_001757"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7449932.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11739..12326)
                     /locus_tag="IVO87_001758"
     CDS             complement(11739..12326)
                     /locus_tag="IVO87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449933.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12501..13823
                     /locus_tag="IVO87_001759"
     CDS             12501..13823
                     /locus_tag="IVO87_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449934.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13978..14754
                     /locus_tag="IVO87_001760"
     CDS             13978..14754
                     /locus_tag="IVO87_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7449935.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14747..15316
                     /gene="rnmV"
                     /locus_tag="IVO87_001761"
     CDS             14747..15316
                     /gene="rnmV"
                     /locus_tag="IVO87_001761"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7449936.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15309..16193
                     /gene="rsmA"
                     /locus_tag="IVO87_001762"
     CDS             15309..16193
                     /gene="rsmA"
                     /locus_tag="IVO87_001762"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7449937.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16644..17273
                     /locus_tag="IVO87_001763"
     CDS             16644..17273
                     /locus_tag="IVO87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449938.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17397..18506)
                     /gene="ugpC"
                     /locus_tag="IVO87_001764"
     CDS             complement(17397..18506)
                     /gene="ugpC"
                     /locus_tag="IVO87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7449939.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18715..19137
                     /gene="mgsA"
                     /locus_tag="IVO87_001765"
     CDS             18715..19137
                     /gene="mgsA"
                     /locus_tag="IVO87_001765"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7449940.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19460..19996
                     /locus_tag="IVO87_001766"
     CDS             19460..19996
                     /locus_tag="IVO87_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7449941.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6515 a         3594 c         4081 g         5818 t
ORIGIN      
        1 ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag tttctttttg
       61 tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat tatttaatgg
      121 cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa ttccttgaaa
      181 cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat tttttaataa
      241 ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa attccaataa
      301 aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat gcggccttgt
      361 acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa aaagcggttc
      421 agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta cagctgactt
      481 atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg aaggaattta
      541 ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg cagaagtcag
      601 tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt caaatggtga
      661 tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc cagctacagc
      721 ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta cagctggaaa
      781 gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag aaatcaaatt
      841 gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt tcccacaaaa
      901 acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata aagctgttta
      961 taatggacca ttcacattag aagattttga tggaccagga acagatacag aatggactta
     1021 taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag
     1081 tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag cagatgacgt
     1141 gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata cttctgttaa
     1201 agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa
     1261 tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag taaaacaagt
     1321 actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg taaaaagtcc
     1381 aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg acaaagataa
     1441 agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt tagaatttga
     1501 tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc aaaactcaat
     1561 ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct ctgtacgtct
     1621 tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg ctgactatgc
     1681 agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc gtggtcgctg
     1741 gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg ctggcgatga
     1801 acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg atatgggtgt
     1861 cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt
     1921 tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag aataatcgtt
     1981 aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc gttctcatat
     2041 tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt agtattccgg
     2101 caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag gaaaattctg
     2161 gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga
     2221 aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat gaccataaga
     2281 atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt tttattaaaa
     2341 taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc atttgattgc
     2401 tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga agtcaaaagc
     2461 tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt ctttttcatg
     2521 atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt gctgccgggc
     2581 acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct aaataaacaa
     2641 gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa tttgcttcaa
     2701 ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt gttagctgga
     2761 agaatcggac catccttgca gttagggtta caggcaatta tctttggaac agtcctaggg
     2821 acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac atcttctaca
     2881 ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt attacaatat
     2941 atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt tgtgtacaca
     3001 atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg gtttatccgt
     3061 acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc aaaaggattg
     3121 agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc attgatgact
     3181 ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga aaatatcttt
     3241 gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga ttatcctacg
     3301 atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt gatcgtggat
     3361 ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg aggataaatg
     3421 gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga atttcttcca
     3481 ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt gaattttatc
     3541 caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat ggttttacta
     3601 ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga tccaactgcc
     3661 caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat cgatggattg
     3721 aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc aaacgtccct
     3781 tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt aagccgctta
     3841 tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact agatgtaacg
     3901 attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg
     3961 caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg
     4021 gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac aaactggata
     4081 ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga atatgtttta
     4141 gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat tttgccgaat
     4201 atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat tttcttcgaa
     4261 gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt aggaacgatg
     4321 ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat tccggcagta
     4381 actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg tgatgccttt
     4441 gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa gacttagaaa
     4501 tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat tttcatttga
     4561 ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca gtgacgacca
     4621 gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa atcctcttta
     4681 aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa
     4741 tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc attgggaagc
     4801 aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga caaaaagctg
     4861 cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc aacagttatc
     4921 cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt
     4981 atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact attcaagcac
     5041 agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt atctttatca
     5101 ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa
     5161 gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat ccctatactt
     5221 ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg tatgctattc
     5281 ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat ccaagaaatg
     5341 aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta tcaaaaacac
     5401 acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg ccagaagaaa
     5461 tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt caaggaggaa
     5521 ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt caatgtagga
     5581 aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga aggagaaaca
     5641 ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac gatcattcgc
     5701 ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac gaagatcaaa
     5761 gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca agatccatac
     5821 gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat tgatgttaat
     5881 ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt gagaactgtt
     5941 ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg gcaacgccag
     6001 cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg tgatgaacca
     6061 atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca agatctccaa
     6121 aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt taaacatatt
     6181 agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac aagtgatgaa
     6241 atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat tcctctacca
     6301 gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc agatgacgga
     6361 caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac tgagcaggaa
     6421 gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt tgctaactag
     6481 gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa gaacaaaaac
     6541 agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg ctgaaaaagc
     6601 ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc gctacattaa
     6661 aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat gcacagctta
     6721 atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca aagcttaaaa
     6781 cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg atctatctgg
     6841 aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat cagcatcggc
     6901 cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa gaattttatg
     6961 tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa tggatcggga
     7021 tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta actttaccaa
     7081 aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc
     7141 gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag aaacaaaaga
     7201 gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat cttgaacaat
     7261 tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt ccaaaagaag
     7321 aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa ttgcgttata
     7381 agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca gaagaaagca
     7441 attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt atcaagggat
     7501 aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa aagtcttaat
     7561 gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg acaaaccaat
     7621 aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt ttttattcag
     7681 aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa gagttgtttt
     7741 tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat tcatggtgcc
     7801 ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt tcaccgatct
     7861 catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt ttataatcga
     7921 tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct ttacctaaaa
     7981 cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat gctttcaaga
     8041 tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca gtgactaaat
     8101 caagcgagat agccacttct ccgctagata aatggatctt gttactatga cgttctagtg
     8161 tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct ccaataaagg
     8221 ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta ctgaatgtat
     8281 gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt gtttctctta
     8341 agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat gcccatgaat
     8401 ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct tcttctttgt
     8461 tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct ggacgttcct
     8521 gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa tataattttt
     8581 cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta aaagtacttt
     8641 ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta
     8701 tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa agcagataaa
     8761 acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata acgatatata
     8821 cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa tgcccatgga
     8881 ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt gctaatgaaa
     8941 gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt ccagtccctt
     9001 actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa gccgtttttt
     9061 agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc ttttaaagga
     9121 tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa aaaagctaac
     9181 agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc gataaaggag
     9241 gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct attatccaag
     9301 cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa ttgctcgtta
     9361 taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac atggtcaaaa
     9421 aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc
     9481 ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat tcattcgcac
     9541 gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat tagaacaggg
     9601 agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag aattctttac
     9661 agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc
     9721 gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc gcatgagcaa
     9781 atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc agccagaatc
     9841 tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc ttgcagtttc
     9901 acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg tggtgtatgt
     9961 atggatcgat gcattatcca attatattac tgcactagga tatggctccg aagatgaaag
    10021 tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt
    10081 tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt
    10141 atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt
    10201 cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt attatttgct
    10261 tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg tttcccgtgt
    10321 caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg ctatgatcaa
    10381 caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat ttgatagtga
    10441 actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga
    10501 attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca aatacattga
    10561 tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt
    10621 catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc caatcatgac
    10681 agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa tgaatcttga
    10741 aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag gtacaccaat
    10801 cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg
    10861 tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac
    10921 gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt
    10981 catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac tagatgctgg
    11041 agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg atccaagcga
    11101 attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca
    11161 aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac aagtaatcga
    11221 agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag ggggtatgac
    11281 actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt aaattccttc
    11341 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
    11401 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
    11461 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
    11521 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
    11581 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
    11641 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
    11701 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
    11761 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
    11821 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
    11881 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
    11941 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
    12001 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
    12061 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
    12121 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
    12181 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
    12241 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
    12301 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
    12361 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
    12421 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
    12481 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
    12541 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
    12601 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
    12661 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
    12721 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
    12781 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
    12841 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
    12901 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
    12961 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
    13021 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
    13081 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
    13141 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
    13201 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
    13261 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
    13321 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
    13381 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
    13441 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
    13501 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
    13561 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
    13621 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
    13681 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
    13741 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
    13801 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
    13861 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
    13921 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
    13981 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
    14041 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
    14101 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
    14161 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
    14221 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
    14281 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
    14341 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
    14401 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
    14461 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
    14521 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
    14581 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
    14641 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
    14701 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
    14761 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
    14821 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
    14881 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
    14941 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
    15001 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
    15061 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
    15121 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
    15181 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
    15241 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
    15301 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
    15361 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
    15421 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
    15481 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
    15541 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
    15601 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
    15661 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
    15721 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
    15781 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
    15841 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
    15901 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
    15961 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
    16021 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
    16081 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
    16141 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
    16201 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
    16261 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
    16321 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
    16381 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
    16441 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
    16501 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
    16561 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
    16621 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
    16681 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
    16741 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
    16801 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
    16861 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
    16921 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
    16981 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
    17041 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
    17101 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
    17161 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
    17221 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
    17281 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
    17341 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
    17401 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
    17461 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
    17521 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
    17581 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
    17641 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
    17701 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
    17761 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
    17821 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
    17881 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
    17941 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
    18001 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
    18061 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
    18121 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
    18181 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
    18241 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
    18301 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
    18361 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
    18421 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
    18481 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
    18541 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
    18601 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
    18661 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
    18721 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
    18781 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
    18841 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
    18901 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
    18961 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
    19021 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
    19081 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
    19141 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
    19201 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
    19261 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
    19321 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
    19381 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
    19441 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
    19501 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
    19561 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
    19621 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
    19681 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
    19741 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
    19801 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
    19861 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
    19921 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
    19981 gatccacgct aaataaaaaa caagcagt
//
LOCUS       DACCQP010000044        19988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCQP010000044 DACCQP010000000
VERSION     DACCQP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.044"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="IVO87_001767"
     CDS             complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="IVO87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7449942.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1656..3077)
                     /locus_tag="IVO87_001768"
     CDS             complement(1656..3077)
                     /locus_tag="IVO87_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7449943.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="IVO87_001769"
     CDS             complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="IVO87_001769"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7449944.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5488..6744)
                     /locus_tag="IVO87_001770"
     CDS             complement(5488..6744)
                     /locus_tag="IVO87_001770"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7449945.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7324..8625
                     /locus_tag="IVO87_001771"
     CDS             7324..8625
                     /locus_tag="IVO87_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7449946.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8694..9293)
                     /locus_tag="IVO87_001772"
     CDS             complement(8694..9293)
                     /locus_tag="IVO87_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7449947.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9547..10428
                     /locus_tag="IVO87_001773"
     CDS             9547..10428
                     /locus_tag="IVO87_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7449948.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10441..12051
                     /locus_tag="IVO87_001774"
     CDS             10441..12051
                     /locus_tag="IVO87_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7449949.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12128..12787)
                     /locus_tag="IVO87_001775"
     CDS             complement(12128..12787)
                     /locus_tag="IVO87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7449950.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13070..13735)
                     /locus_tag="IVO87_001776"
     CDS             complement(13070..13735)
                     /locus_tag="IVO87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449951.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13882..14487)
                     /gene="dhaL"
                     /locus_tag="IVO87_001777"
     CDS             complement(13882..14487)
                     /gene="dhaL"
                     /locus_tag="IVO87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7449952.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14515..15498)
                     /gene="dhaK"
                     /locus_tag="IVO87_001778"
     CDS             complement(14515..15498)
                     /gene="dhaK"
                     /locus_tag="IVO87_001778"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7449953.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15500..15895)
                     /gene="dhaM"
                     /locus_tag="IVO87_001779"
     CDS             complement(15500..15895)
                     /gene="dhaM"
                     /locus_tag="IVO87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7449954.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(15985..16944)
                     /locus_tag="IVO87_001780"
     CDS             complement(15985..16944)
                     /locus_tag="IVO87_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7449955.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            17114..17434
                     /locus_tag="IVO87_001781"
     CDS             17114..17434
                     /locus_tag="IVO87_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7449956.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(17546..18415)
                     /locus_tag="IVO87_001782"
     CDS             complement(17546..18415)
                     /locus_tag="IVO87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7449957.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18511..19815)
                     /locus_tag="IVO87_001783"
     CDS             complement(18511..19815)
                     /locus_tag="IVO87_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7449958.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         5695 a         4133 c         3651 g         6509 t
ORIGIN      
        1 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
       61 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt
      121 gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt
      181 caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca
      241 agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata
      301 atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct
      361 agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt
      421 tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag
      481 ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga
      541 acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa
      601 atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc
      661 tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc
      721 gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat
      781 gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat
      841 gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct
      901 agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat
      961 gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata
     1021 cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg
     1081 gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa
     1141 aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt
     1201 gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa
     1261 accataatcg tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa
     1321 agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc
     1381 tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg
     1441 atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac
     1501 acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta
     1561 tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa
     1621 tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct
     1681 acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg
     1741 accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca
     1801 aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt
     1861 ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc
     1921 aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt
     1981 gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata
     2041 acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat
     2101 ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca
     2161 gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt
     2221 ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt
     2281 gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc
     2341 aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct
     2401 ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca
     2461 gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag
     2521 tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg
     2581 aacatgatga ctactgggat atatacccct agccatacac cgatgttccc gccgactttt
     2641 gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct
     2701 aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt
     2761 cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca
     2821 acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct
     2881 tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa
     2941 actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc
     3001 cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt
     3061 acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct
     3121 ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag
     3181 tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag
     3241 gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt
     3301 tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt
     3361 tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt
     3421 ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt
     3481 tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt
     3541 ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca
     3601 cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt
     3661 tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa
     3721 cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat
     3781 gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga
     3841 catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat
     3901 aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag
     3961 agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat
     4021 agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct ttatacgcac
     4081 tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa
     4141 catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc
     4201 cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata
     4261 cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta
     4321 caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt
     4381 caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta
     4441 aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca
     4501 accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt
     4561 cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta
     4621 attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca
     4681 atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa
     4741 cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt
     4801 cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca
     4861 tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt
     4921 aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata
     4981 gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg
     5041 taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca
     5101 attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg
     5161 catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata
     5221 tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg
     5281 cctgattttt aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa
     5341 taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg
     5401 acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc
     5461 tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt
     5521 cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa
     5581 atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg
     5641 tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg
     5701 tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt
     5761 tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc
     5821 ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt
     5881 ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt
     5941 aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa
     6001 ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa
     6061 tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg
     6121 tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc
     6181 accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag
     6241 gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc
     6301 ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa
     6361 agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa
     6421 caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg
     6481 caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat
     6541 cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa
     6601 atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt
     6661 ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca
     6721 agttaattca tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag
     6781 gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt
     6841 cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga
     6901 tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc
     6961 tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac
     7021 attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat
     7081 catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt
     7141 ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa
     7201 ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat
     7261 tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg
     7321 tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt
     7381 ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct
     7441 gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact
     7501 caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa
     7561 gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa
     7621 gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg
     7681 atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg
     7741 gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta
     7801 gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt
     7861 gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa
     7921 tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa
     7981 aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc
     8041 gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc
     8101 ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat
     8161 gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac
     8221 ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa
     8281 gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca
     8341 aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa
     8401 aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct
     8461 tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc
     8521 gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc
     8581 aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat
     8641 gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc
     8701 ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt
     8761 tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag
     8821 aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca
     8881 agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt
     8941 tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga
     9001 taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca
     9061 atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc
     9121 agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca
     9181 gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct
     9241 gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga
     9301 gcctcccgta attttaaact tttataatat atatagttca aatttattct gaggtttatt
     9361 ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat tcattgacag
     9421 aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata aaaaggtata
     9481 aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg
     9541 gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa
     9601 gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca
     9661 aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc
     9721 ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt
     9781 ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc
     9841 gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa
     9901 cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa
     9961 gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact
    10021 tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa
    10081 cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat
    10141 aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt
    10201 catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa
    10261 ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac
    10321 gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct
    10381 attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa
    10441 atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg
    10501 aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg
    10561 tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg
    10621 gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga
    10681 gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt
    10741 ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt
    10801 tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc
    10861 aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc ggattcgatc
    10921 actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt
    10981 gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct
    11041 ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc
    11101 ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac
    11161 tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt
    11221 ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat
    11281 gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca
    11341 atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat
    11401 atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc
    11461 tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct agctgctatt
    11521 ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag
    11581 ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt
    11641 ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct
    11701 tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc
    11761 ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga
    11821 atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga
    11881 attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg
    11941 ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg
    12001 ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc
    12061 atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt
    12121 tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc
    12181 tttttacata accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg
    12241 taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg
    12301 tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca
    12361 aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca
    12421 gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa
    12481 tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc
    12541 tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag
    12601 ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg
    12661 gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt
    12721 cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat
    12781 gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt
    12841 attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca
    12901 aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa
    12961 ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca aaccttcact
    13021 cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt
    13081 tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca gcggttattt
    13141 gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga aaggttcggt
    13201 ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag tcagacaaag
    13261 ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc ttgatatcga
    13321 ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca atgctaccat
    13381 atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt ttttgattca
    13441 acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac gcgctccaaa
    13501 aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg ctcatgccgt
    13561 acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt tgccagtgtt
    13621 ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct ttgagctgtt
    13681 tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt tccattgttt
    13741 cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac aaatagaggt
    13801 tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac tgcgatgagc
    13861 atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa
    13921 aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga
    13981 agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc
    14041 ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt
    14101 tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac
    14161 ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt
    14221 catgaatgca gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat
    14281 agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc
    14341 ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc
    14401 tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc
    14461 ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca
    14521 tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat
    14581 cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa
    14641 aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg
    14701 ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt
    14761 cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat
    14821 cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc
    14881 tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc
    14941 gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc
    15001 accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg
    15061 ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc
    15121 tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc
    15181 tgtataattc ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt
    15241 ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat
    15301 cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa
    15361 accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac
    15421 aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc
    15481 attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta
    15541 agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca
    15601 ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg
    15661 ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat
    15721 cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta
    15781 ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt
    15841 taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa
    15901 tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca
    15961 agtatataat aaatttttcg tttgtcaaat taagctagac aaatcatctt tacctacgta
    16021 actcaaaaat acttccaatg gtgtgcgata gtgtaatgat tttctaggaa tatgatttcg
    16081 ttttgatgcc accgattgga taaaggtttc ggatacctta ttaaaatcca tagctttata
    16141 taagccatct ttacgtaata agccattcga atgttcattt agtcctcgtt gagaaggtgt
    16201 tcctggatca gcgaaataaa tcgaaatatc attggtatta ctaatctttt tccaattcga
    16261 gaactctttt ccacaatcaa atgtgatgga tttaaacaag tgtttcggta cagattgaaa
    16321 ccattgattc agtctattct caatatctat agcttgccta ccagcgggtt tcaaggtgat
    16381 gatcacttta gataatcgct caactaacgt gatcaccgca cttttatgtt ttccaccgac
    16441 aatggtgtca ccttctaggt gaccaaactc atgattgaac tgtatataat cagttgttct
    16501 atcctgaatc gaacggcgaa aagcttgttt tcctcttttt tctttatgtc cgtttggttt
    16561 tcttttccct ttcattggta aatcgtgagg gtcaaaggtt ttatctttaa acatccgata
    16621 tagtgtacgt accgaacaag caataggaaa ctctgctcga ccaataagga catctggagt
    16681 ccacccttga gccacctttt tttgaatgta ttcttgttgc ccatctggta acacaatcgg
    16741 acgtcttccg cagttacgtt tgtttttctt atattgttca tagtagctta ttgcgcttcc
    16801 accagattta agaaaacgat agacattata aatggtttgt cgagaacgtt gtaccagatg
    16861 ggctactttt gaaacagagt gtgattgatt gaaataagct tctatcatta cgagttcagt
    16921 tggtgtaaga tgggtatagg tcatttgtac tcactcctta taattctttc gacggaacta
    16981 ttttgagtgt agcacaaatg acttttttag ttgtctagct taattttaca atcggcgatt
    17041 tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga
    17101 gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc
    17161 ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga
    17221 ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg
    17281 tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc
    17341 gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt
    17401 cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta
    17461 aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt
    17521 actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg
    17581 cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt
    17641 ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg
    17701 tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga
    17761 aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc
    17821 tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct
    17881 ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga
    17941 tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt
    18001 tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga
    18061 ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt
    18121 ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta
    18181 ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag
    18241 tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt
    18301 ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg
    18361 aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta
    18421 ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac
    18481 aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca
    18541 tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt
    18601 tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa
    18661 ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat
    18721 ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc
    18781 ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat
    18841 tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa
    18901 aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca
    18961 acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat
    19021 ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc
    19081 ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat
    19141 cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat
    19201 gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt
    19261 ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata
    19321 attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat
    19381 caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt
    19441 ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg
    19501 tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt
    19561 cgtcatcaaa tactcgattg tatcttcgat cacaatttct tcattatggg ctggcaccat
    19621 gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt
    19681 gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta aaatcggata
    19741 agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg
    19801 ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc
    19861 gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt
    19921 gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca
    19981 tcttcata
//
LOCUS       DACCQP010000045        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCQP010000045 DACCQP010000000
VERSION     DACCQP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.045"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IVO87_001784"
     CDS             133..3660
                     /locus_tag="IVO87_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7449959.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVO87_001785"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVO87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7449960.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVO87_001786"
     CDS             7448..7789
                     /locus_tag="IVO87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7449961.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVO87_001787"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVO87_001787"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7449962.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVO87_001788"
     CDS             9146..11566
                     /locus_tag="IVO87_001788"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7449963.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO87_001789"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO87_001789"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7449964.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVO87_001790"
     CDS             13000..13737
                     /locus_tag="IVO87_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7449965.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVO87_001791"
     CDS             13751..14566
                     /locus_tag="IVO87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7449966.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVO87_001792"
     CDS             14579..15226
                     /locus_tag="IVO87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7449967.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVO87_001793"
     CDS             15242..15898
                     /locus_tag="IVO87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7449968.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVO87_001794"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVO87_001794"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7449969.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVO87_001795"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVO87_001795"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7449970.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVO87_001796"
     CDS             18240..18758
                     /locus_tag="IVO87_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7449971.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVO87_001797"
     CDS             18865..19365
                     /locus_tag="IVO87_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7449972.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACCQP010000046        18630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCQP010000046 DACCQP010000000
VERSION     DACCQP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.048"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IVO87_001798"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IVO87_001798"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7449973.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="IVO87_001799"
     CDS             1131..1406
                     /locus_tag="IVO87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449974.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1833..2288
                     /locus_tag="IVO87_001800"
     CDS             1833..2288
                     /locus_tag="IVO87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7449975.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(2368..3117)
                     /locus_tag="IVO87_001801"
     CDS             complement(2368..3117)
                     /locus_tag="IVO87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449976.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(3118..3444)
                     /locus_tag="IVO87_001802"
     CDS             complement(3118..3444)
                     /locus_tag="IVO87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7449977.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            complement(3592..4032)
                     /locus_tag="IVO87_001803"
     CDS             complement(3592..4032)
                     /locus_tag="IVO87_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7449978.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            complement(4113..5510)
                     /locus_tag="IVO87_001804"
     CDS             complement(4113..5510)
                     /locus_tag="IVO87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7449979.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            5663..6934
                     /gene="celB"
                     /locus_tag="IVO87_001805"
     CDS             5663..6934
                     /gene="celB"
                     /locus_tag="IVO87_001805"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7449980.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7001..7780
                     /locus_tag="IVO87_001806"
     CDS             7001..7780
                     /locus_tag="IVO87_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7449981.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            8158..9132
                     /locus_tag="IVO87_001807"
     CDS             8158..9132
                     /locus_tag="IVO87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7449982.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            9185..9790
                     /locus_tag="IVO87_001808"
     CDS             9185..9790
                     /locus_tag="IVO87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7449983.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(9884..10636)
                     /locus_tag="IVO87_001809"
     CDS             complement(9884..10636)
                     /locus_tag="IVO87_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7449984.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(10650..10910)
                     /locus_tag="IVO87_001810"
     CDS             complement(10650..10910)
                     /locus_tag="IVO87_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7449985.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            complement(10932..12293)
                     /locus_tag="IVO87_001811"
     CDS             complement(10932..12293)
                     /locus_tag="IVO87_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7449986.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(12366..12665)
                     /locus_tag="IVO87_001812"
     CDS             complement(12366..12665)
                     /locus_tag="IVO87_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7449987.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            complement(12678..13136)
                     /locus_tag="IVO87_001813"
     CDS             complement(12678..13136)
                     /locus_tag="IVO87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7449988.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITNYI"
     gene            13360..14151
                     /locus_tag="IVO87_001814"
     CDS             13360..14151
                     /locus_tag="IVO87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7449989.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            14117..15070
                     /locus_tag="IVO87_001815"
     CDS             14117..15070
                     /locus_tag="IVO87_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7449990.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(15468..16442)
                     /locus_tag="IVO87_001816"
     CDS             complement(15468..16442)
                     /locus_tag="IVO87_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7449991.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            16557..16991
                     /locus_tag="IVO87_001817"
     CDS             16557..16991
                     /locus_tag="IVO87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7449992.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(17116..18102)
                     /locus_tag="IVO87_001818"
     CDS             complement(17116..18102)
                     /locus_tag="IVO87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7449993.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            18530..>18630
                     /locus_tag="IVO87_001819"
     CDS             18530..>18630
                     /locus_tag="IVO87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7449994.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEI"
BASE COUNT         5725 a         3714 c         3194 g         5997 t
ORIGIN      
        1 ctttttcaca acctctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat
     1501 aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca
     1561 cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg
     1621 agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt
     1681 agtctagttg tttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg
     1741 ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta
     1801 acaaattaat ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac
     1861 atagttacca tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa
     1921 ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag
     1981 aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc
     2041 aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt
     2101 tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat gaagatagca
     2161 tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt
     2221 ttacatgggt aagcaatcta gaaaattcta cggatttaga tgatgaattt ttaaattatt
     2281 atatataaaa aagactgtga caaaagcgtg tcacagtctt tttttctacg ccggataaat
     2341 agaatatttt tacaaatctg aatacattca ccagtaataa gcgctttgag tgaattgacg
     2401 ataagaatga cggactagac tgccataaat gatcaagaag acacccgttc ctaccgtaac
     2461 aaaaaatata gaggcaccga tactaccatc catataccac attaaagaag ccaaaagtgg
     2521 gaaaagtgaa aaaatacata atccaattcc tgcaatcagt gagaagccaa aacttcgctg
     2581 atactcttct tctttttctt ttgcttctgc taatactttt tcaggtattt tccgatggtg
     2641 caaaaagtga tttaaacgtg tgattttcat accagaagca ataaaaaaac cgacagctaa
     2701 tgctataccg aaaatcagac aagaaatacc catgaacaca tatcctgctg aacaaaagaa
     2761 aagaaaactt gcaaaagata agctaatgaa tagaacacct gatgccactt ggagacttgc
     2821 cgctctgtat tctttccagt agttttctgc ttcgcttaaa taaatagaag ctgtgtttgt
     2881 ttccgtctcg tcagcttgat gttttttttc gacatctagt tcttctaata attcatcgat
     2941 tgaaccaaat gtcgaaatta ctgtaccgat ggcctcctgt tcattttttc cttctcccat
     3001 caattcatga taatggtctt ccatatttgc caacaaatca gttttcagct tatctatctc
     3061 ggtactttga ggtactccga taaaaagact gtctaaataa ttacggattg tatccattta
     3121 atttccctcc aaaaattgat cgactacgtc tttcgtcgtc agccactctt gctttttttc
     3181 ttctaaaaga ttacgcccta tttcagtgat tttataatat gtccgctttt ttccatgagt
     3241 cacttctcca ggatacgaaa caatcagttg attcttttct aaacgggcaa aaacggagta
     3301 aagtgttgtc tctttgatca cataggaatc atttgtcctc tcacgaatcg ttttagaaat
     3361 ttcatatcca taagaatcgt ttttgttaag gatcgataaa ataatcgcat cattgtatcc
     3421 gcgtattcca tcacttgaaa tcattttctt tcctccatat tactccatct gtcgttgtaa
     3481 ttagaatagc atgattactt catcaggtaa agtaattttt tacaaaaaaa taaaaggaac
     3541 agaaagcaaa agaagcgtta tgcttttttg ctttctgttc ccttaattta tttattttac
     3601 aaccatgacg ttacatgatg cgtggttgac tacataggaa gtagtggaac ctactagtgc
     3661 acgttctacc gcattcagac ctgtcgcccc catgacaatc agatcgattg gttctttcga
     3721 ttcatggaaa ttagcaatca gtgtctttgg ataaccgtaa agaacatatg tctcgatatc
     3781 tgtcaggcct ttttcaattg ccaagtttcg atgttctttc atctggtttt ccgcaacctc
     3841 atccaattct tcctgaaggc ttgttgtaac aaatgcaaaa tcaccaaagt aattgcctgt
     3901 tacttcattg acatacagga tgtgcaacgt tgcctgattt cgtttggcaa tcgtgattgc
     3961 ctcaagaaat gctttttctg ccttttcaga accatcaatt gctactaaaa tattacgata
     4021 agtatctacc attgtaaacg cctccttcac tgttagtgta gcacgattac agtgttagaa
     4081 caaacatttt gtgaacgttt tatcaaaaaa tatcaggcat caaatccgtt tttttcagag
     4141 accgttttaa accagcgacc tgattttttg atcgtccgtt taccgtcttg tgccaaatca
     4201 acagcgataa agccatagcg attcttataa gcatttgtcc aagaccagtt gtccatacat
     4261 gtccacatgt ggtaacccac acaattggtt ccttcttgga ttgcttgatg gacgtatttc
     4321 aaatgatccg taacaaactc gatccggtaa tcatcttcaa taattccttc gctgttaaca
     4381 aatcgttctt ctccttcgac ccccatccca ttttcagaaa tatagcattt gatattacca
     4441 tagttttctc ttgtattcgt taaaatatca tagattcctt tttcgtaaat ttcccatcca
     4501 cgatatggat tcattttttt attgggcatg tcgtaattat caaaataatc atctggcatg
     4561 gggctatgtt cgtgattgat tggagtttct tttgctttga ttcgacgagg ctgatagtaa
     4621 ttgattccta gcaggtcaat cgtgttttca cggatgagtt ctaggtctcc ttcatggatc
     4681 tctggcatta tagccaattc tttgacaatt tcgatcaact cttgtggaaa ctctcctttc
     4741 actgcagggt caaggaatga tcggttgaag aatgcgtcag cgatcttcgc cgcttttaca
     4801 tcttctgggt tcgtttcatc tctcggatag cttggtgtca gattcaagat gattccgatc
     4861 tcaccatcat gtccgccttc atggtaggct ttgattacct ttgcgcttgc tagattctca
     4921 tgatagccta cttggaccgc ttctttcata ttgatatgat ttggatagtg gaattgatac
     4981 aagtagcctc cttctacagg tacgattggt tcattatgag taaaccattt tttgaccaga
     5041 tctccaaaca gttcaaagca cgctgctgca taagaaacat aagcgtcgac cgtttctctt
     5101 gataaccatc cgcctttttc ttgcagtggt agtggcatat cgaaatggta aaggttcaca
     5161 aaaggttcaa taccattatc cagcaattca gtaaaataat ctcgataaaa agaaacagct
     5221 ttttgattga tttcacctgt cccttctgga atcaaatgac tccattggat agatgtacga
     5281 aatgtgttat ggccagtttc tttcattaac tgaacatctt ctttgtattt tgtgtaaact
     5341 tgcgaagttt tttcaggtcc tacttgatcg aagaattttt caggctctaa ttcataccaa
     5401 tgatcccaaa tattttctcc tttaccatca tcacttactc ttccttctgt ttgcggtccg
     5461 ctagcagcag aaccccacca aaaattttct gggaatgtgt aggttttcat ttttttactt
     5521 cctctctttt ttcttgatac cgtaatcata acgaacccta aaaaataaat caatactttt
     5581 tatgaaaatg actagacatt taaatttttt ggcgttatac tttgtatgta atcgattgca
     5641 aataaatttg aggtgaataa aaatgaacgg ttttatggac aagctttctg agaaaatcat
     5701 gcccttagct aatttattag ggcaaaatcg ttaccttaca gtgctgcggg atgctttcat
     5761 gctttccttc cctttaacga tgttcggttc gatcgttgtc gttatcaata acttgccttt
     5821 tttcagtgac gcaacaaagg gaactttatc gaatttattc ggtaatgggc aaaatgcaac
     5881 aatgagcatc atgtcggttt ttgttacttt tggtatcggc tattatttat caaaatctta
     5941 tgacgttgaa ggtattttcg gtggtgccgt gtcttttgcc agcttcttga ttcttacgcc
     6001 ttttgcaatg aaaaccgctg gcggtgaaga tgtgactggt gtgctttcac ttgatcgtct
     6061 tggcgcaaaa ggaatgttca ttggaatgat cgctgctttc ttggctggag aaatctactg
     6121 cagaatcacg aaacgcggct ggcagatcaa aatgcctgat ggagttccgc ctgcagtaac
     6181 aaaatcattt gccgctttga tccctgctgt agtgacacta actattttct tagtaatcaa
     6241 tgctgtgatg actggtattt tcaatgctaa cttacatgac gtcgtttatg aagtgatcca
     6301 aaaaccatta actggtttgg gaagtagttt acctgcaaca ttgattgctt tgttctttgt
     6361 ccaattctta tggttcttcg gacttcacgg acaaatcatc gtcaactctg taatggaccc
     6421 gatttggaat acactgatgc ttgataactt agaagcttat caacatggaa aagaattacc
     6481 acacatcatc acaaaaccat tcatggaaac cttcactgtt ggtattggtg gctctggaat
     6541 gactttagct gtcgttttat tgatggcctt tgttcttaaa aagaaacagt atcgtgatgt
     6601 tggacgatta gctttagcac ctggtatttt caatgtaaat gaaccagcga tcttcggtct
     6661 accgatcgta ttgaatgcaa caatccttat tccttgggtt gtggcacctc taatcgtaac
     6721 gacgttcaat tatttggtaa tggctgctgg acttgtccct gcaccaactg gcgtctctgt
     6781 tccatggact gtgccaatca ttgcaagcgg tgtcttagca acaaattcat ggcttggtgg
     6841 tatgctgcaa gtcatcgact ttgtcatcgt tgcctttatc tggtacccat tcctgaaagt
     6901 actagataaa caaccagatt tagatattgc ttaatagatc attaaggggt tgtgacaaaa
     6961 acttgtcatg acctcttttt tcgaataaac aatgttccag atgctacttc gagttgtcgg
     7021 aagcataatc tttcgttata ctctagaaga ggtgaataac gtgccaaaat acgaagaaat
     7081 cgcaaatatt ttaagagaac gtatccgttc aaaagagtat cctctcgata gttttttgcc
     7141 taatcaagtc gacttggtaa aagaatttgg tgcgagccgg atgacaatca aaaaagccat
     7201 caatatctta gcaatggaag gacttgtcta ttcgcagcga ggatcaggaa cgaaaatctt
     7261 gaatcatcca tttatgcaaa aagacacatc gcccgctaac gagtatgaag gtttgagtct
     7321 ccaaatgaaa cgcagacatc gttctttgaa gagtcaaatc attcattttg atgttgaatt
     7381 ccctaatcaa tatatccaag aacgattgat gatcgatgcc gagcaacctg tctacaatat
     7441 tcaccggtta aggatcttag atggcgagcc atatgtttta gagcatacct ttatgccagt
     7501 ccatcttgtg ccgggtttaa aaaaagaaca tttgttagcc tctatctatg atcatcttca
     7561 tcaagaattg aatgtccatt ttgcaggggc ttatcgtgct atttctgcag ataaaagtga
     7621 tgaatatgat cagcaatatt tgaactgtgc agaaaacgat cctgtactgg aagttcagca
     7681 agtcgtctat ctcaaagatg gacagccgat tgaatattct tccagccgaa accgatttga
     7741 tgttcggaac tattctgtgc tggatgtgcg ttcaacttaa ccaaagaggt tgtgaaaaag
     7801 ccgtttacca agagatcgtg acagtggtaa gcagtttcaa gtaacaagaa gagatcgtga
     7861 aatcaacatt ctattctatc ctaagcatta gctcaaaaat gaaaaaataa ctcagcataa
     7921 aaagacttat gaccacccgt aatgattcta aagttagctt tttaaagcta gtttttttga
     7981 aatcgataca gctagtcgta agtcttttta ttttattgat ccgtccctcc tcttttccat
     8041 tcttgattcc ttcctgatac tttagaagac aaattaattg caaaatagag atgcaccctc
     8101 tatactatgt ctaaatcaac tacttacttt ttgtaagtta acaggaggaa atttatcatg
     8161 gatacacaaa tcagaggaat tcaccacgtc acagcaatga catctagcgc tacaaaaatc
     8221 taccgctttt ttacagatat tttaggaatg cgcttaatca aaaaaacagt taaccaagat
     8281 gacatcgaaa cttaccatct ttactttaca gacgatactg gtgcgccagg aacagatatg
     8341 actttctttg attttccaaa tcagccaaaa ggaaaaaaag gaacagatac gatctctcgt
     8401 gcttctttcc gtgtgccaag tgacgcagca ttagactttt ggttggaacg ttttgaaaaa
     8461 tatgacgtta ctcattcagc aatcaatgaa cgtttcggca aaaaatatat cgaatttgaa
     8521 gatttcgata atcagtatta tcaattgatc tctgacgaaa acaatcaagg tgtagcagct
     8581 ggcattcctt ggaaaaacag caatgtaccg acagagtttg cgatcacagg acttggacct
     8641 gtctttgtcc gtgtcagtca attagagcat atccgcttga ttcttgaaaa aatcttagga
     8701 ttcaaagaaa cagaccattc aggagatttc tacttatttg aaatcggtga agggggaaat
     8761 ggtgcaagca tcattattga acatcggaca gacttgccaa aagcaatcga aggatttgga
     8821 aatatccatc acttagcatt gcgtgtagca gacaaagaag cattggaata ctggattcaa
     8881 gtaatcaata agattgaatt gccaaattct ggtttcgtcg atcgtttcta tttccaatct
     8941 gagtacttcc tagccgctca tcacgtactg tttgaattag caactgatgg tcctggattc
     9001 cttgaagatg aaccagcaga atcagcagga gaaaaactat ctcttcctcc attcttagaa
     9061 gaacgtcgtg aagccattga agcttatgtc cgtcctttcg atacaagtga tgcaaataaa
     9121 aaacgtgaat aatctcttca tgctacttca tttgcatcta tagaaacaga aaagagggaa
     9181 caaaatgacc tacatttttg aaaaaggaac aagtggcaag catttgatct tacttcatgg
     9241 cacaggtggc gatgaacatt ctttactgga tatcgctcac tttcttgcac caaacagtac
     9301 attgctttct tttcgtggca cggtccaaga agatgggatg aatcgttttt tcaagcgtaa
     9361 tggattgaat caattcgacc tggacagttt ggaaaaagaa acagataaac tccatgaaaa
     9421 aatcaaagaa atcagcgaaa aagaactgat accgttaagt gactggctgc ttgtcggcta
     9481 ttcaaatggc gcaaacatcg ctgctcattt attattggag cgtgagaccg agctaaataa
     9541 agggttgttt ttccatccga tgtctctagg tgttcatcaa agccgttcca cattagagga
     9601 taaaaccgtc tggatttctt atggtgataa cgatccaatc gtttcccctg cttcctttaa
     9661 agaattggcc gatcaattcg aaacacgcca agcaaatgtg actattcagc taactgtaac
     9721 aggtcatcag ttgacgatgg acgaaatcaa gcaagcgagg gaatggacag accacctctt
     9781 aactaaataa aagaaaagaa ctacgactta cataatacaa cctgaagcag atatcgatct
     9841 ggatcagatt ttgtgaaagt cgtagttttt tgtgtcagat aggttagtct ccaccagctg
     9901 caataacaga tttattataa atagccgttt tatgtttcaa ctcttccaat cccccgattt
     9961 tctcaccttt tgaataaaaa ccgtcagtaa tccctaatgc ttgaaaaatc gtctgcttgt
    10021 aggcaatttc tgcattccat tcaactgtat caaggatttg cagtgcttta tggatagctt
    10081 cgattcctga ttctccacct gtgttgatca atacgccatg agagttgagc agcaacatat
    10141 gcggtaattc gagatgctgt ttttttacat agtcgcttgt caattcagca agttcttcag
    10201 agcaattagg ctcaaagtcc atgacaggaa tctcttttac tttagctgtg gcttctgtca
    10261 aattaggcat aggcaaccca gtagtcgccc agaacattgc attaggcgca tgagcatgta
    10321 ggacagcttt gatagctgga ttagtctcat acatcgcttc atgcatattg atttcacgag
    10381 ttaaatgacc gactccgtca acgattctac gcgttcttgg ttctactact agaacctgtg
    10441 caggagaaat ctctcctaga taggcttctg acatcatggt cggcgtcatg atgatgtagt
    10501 ctttcccttg atcatcagtg gttttaaagg aaaaattccc tcctgcgaca tttgtgtttt
    10561 tcctttcgaa aatcactctt acgatacgtg ccatgtcctg tcgttctctc tcaaataaca
    10621 tacgtccatc tgacataatg acccctccat tatcttttta taaaactttt ttctctcaat
    10681 atttctttgg tagcttcttt tattttcatt gattcttcta ttttccgttt tcttttttca
    10741 tcgtactctc tgaataaaac gtgatttcca tatttataga tcactagtgc gaaaaaaatg
    10801 accaatagat tgatgatgat attctgagta aagagtccat atagaaatag cagaagtgta
    10861 ccacaaagag cgactgtcca ttttatttta tctgtctctt ctcttttcat tactttccca
    10921 ccatttcaat gctatttttg tagttcagca taacgttcac tcggtacttt cgcttttatc
    10981 atatatttat aaagtagaac aaacagtcct agaaaaatca cgcatcctac aatagcgaga
    11041 atattcctat taaatgcctc cgcaaataaa accctgaact ccggcccttc tatggatgac
    11101 catgtgatca actgtccttt cttgattccc tcgactgctc cgcttttttc tgctaaatca
    11161 ctgagtattg gcgcaaggta agtggccccg tacaaataaa taggcatagt gatgattccc
    11221 aagatcagca tacgagccaa gtttcctcct gttaaaagta atccgccgac cgcaatcgaa
    11281 tagttgataa tccctgctaa tggcaatact tggtttcctg gcaaaataat ggcataacca
    11341 ataaacacag gaacaagtaa aatagcagta acccaaattt ctgaacgacc agctaatatc
    11401 ggccagtcca atccgatata tacttcacga tccttaaacc gctttttcat aaattctgac
    11461 atagcgtctg ctagaggaga caaagattgc atgaacattt tagagatcat aggaaacaaa
    11521 cttaatgctg tagctgtttg gatcgctaga ttaagagaac ctgaaacgcc atacgcagca
    11581 gcaaagccaa gccccaatcc aataatgaat cccattacag agttttcaga gaagatccca
    11641 attttcttct tcaatgcatt cgtatccgct cgtttattta aagcaggaat tttatccatg
    11701 atcatattga aaggcagcaa caagacagcg gcagatgtca taaaatgagt cactgttact
    11761 aatggaatcc ctgtgagatc ttgtatatgt ttttgaaaca tatcaccaag ctttaattcc
    11821 aatataactt ggaccattgc agtaagaaag cctgcagcca aactaccgga aacataataa
    11881 actaaataag cagttaaagc tttcccccaa acattccaca tatcaacatt taacgtcttc
    11941 gtccaattaa aaagtaacag tacaaaattg accccaatcg ttactgcaaa aaatataaaa
    12001 gcataactcc acgaccatgt gatgcttgct gcacctgtcc acccaagatc aagtgcaggc
    12061 aaactgattc ccgtattttt agccaatgct tctgatgctg gacttactgc gttagacata
    12121 tagccaatga ccatagacat tccgctaaaa gcaatcccta aagtcaaagc agacatgaat
    12181 gcttttttta atttcatccc tgccaacaat ccaagtatca aaataatcag gggaacaaaa
    12241 atggctgatc ctaagttcaa aacataattg ataatggttt ggaaaacttc cataaataaa
    12301 ccttctttct ttctaaatat agtaaataaa actatgaacc aactttttta tccaacaatc
    12361 ctcctttact gtactaactc tttgatactg tcataaatag gttctacacc cattccagtt
    12421 aaaaatggaa ttcctggaaa tacttcaata cccaaatctt ttgcttgttc aagtacttcc
    12481 tcatcaggtt gtgcgacata cacatagatt ccagcagtta gaagctcatt ttgaatcgat
    12541 ttgtaatcaa ctgcttctac aggaaatgcg atgccgtcat cttctaacat actttggatc
    12601 ttactagcga ttgtctgact cgttgctact ccacttccac atgcgacaat taaccgattt
    12661 ttcatttttt cttcctccta aatataattt gtaataagtt cgtatatgtt ttgcggtgtt
    12721 tcttgtttct tgtaattttc cacgaatgtt tcatccatga ataaattcat aaacgcttga
    12781 agtaatccta cttgttcttt tggttctttg attcctaaaa aaaatagatt ttctacagtc
    12841 atttcctgat tatcccccat ttgataaaaa taaattgggt tcttcagctg cataacaaaa
    12901 acaaatggct tttcgacata ttcacattcc gaatgtggca gcgcgatatt taaatactta
    12961 gtaatcaatc cagtaggaaa ttctctttct cttgcaatca gtccattcaa ataattctca
    13021 ttgacgtacc ccattttttc taattgttgt gcagcgatta aaaaagcttc ttctttattt
    13081 tttacttcta ccgctaattg aatcaaacta ggaacgaaca tctctttata tttcattcca
    13141 acctcctcct ttttgattaa gtttgttaat ttattgttct actaagttca tctttgtttt
    13201 acaagaacat tatatttctg ttagtttgtt ttgtcaacgc tttcaaaaga aatttatttt
    13261 gttaaatttg atctacttga aaataaaatc gtttatctat ataattaaga agaacaaagt
    13321 cgaacaaaaa ttgactttta tgttaaagga gtaattcata tgctgaaaaa agaaagacaa
    13381 gaacaaatca ttacattatt agagaaagat ttttatttaa ctaattcaga aatcgctaac
    13441 aaattaggcg tatcagaaat gaccatacgc cgagatgtca ctgaattagc aaaaaaaaat
    13501 aaagtaaaaa agctatatgg tggcgttgaa cgaaaggaac cttataatcg agaactatcg
    13561 actcaagaaa agattgatac gaatgtagcc gaaaaaaaat atattggtca agtcatgaac
    13621 agtatgattc ccgataattc tactatttat gtcggtgctg gaacaacgat cctatatgca
    13681 cttcctttca ttcaaaagaa aaatctattt gtaattacaa acagtttgat tgctttcaac
    13741 tatttaaaga atcatacaag ttatcggatt ttacttactg gtggcgaatt ttctcctatt
    13801 acagaagagt tcattgggga agttgcagaa agatcttttg aaagtttgaa tgtcgatatc
    13861 tctttcgctg caacaaacgg catatttgat aacaatgtga caaccgctca atttattgag
    13921 ggaaatattc agaacgcagc gctcaatgca gcaaaaatca aatgcattgt tgcagattat
    13981 tcaaaattaa ataaatcaga cgtctatact tttagaaaat taaccgattt tgattatcta
    14041 ataaccgata atcatttatc agaacaggat ttcaatcatt attcaaaata cttaacaata
    14101 ttaaaggagc ccaaaaatga tactgacaat cacgatgaac ccatccattg attccattta
    14161 ttttacaaaa cactttgaat taggtacgat gacacgcttt gattctccgg tacgctatgt
    14221 aggcggcaag ggaatcaatt gcggacgagc ttctgctcta ctaggttcat cagttatttt
    14281 aacagggttt ttgggcggtt attttggcag tatgattcaa gatatgctga aaaatgaaaa
    14341 tttgtttgat ttgcattttt tacctattgc agaagaatca agaggtgccg tcacgatcat
    14401 gcatgatgat gaaacgcaga cagagctagt ggaagctggt cctgaaatag cagatactac
    14461 tatcaaaaaa ttaatgaatg atatcaaact aatcagtaaa aataattacc tttcagtcat
    14521 tacaataaat ggttctgtta acaatccaga tccctttttg tatgctaaat tattacaata
    14581 tatcaaagaa caaatagatc cttccaccta tatattaatg gatatatctg gacaacatct
    14641 ccgtgaagtc ttagcaagta agtataaacc gatgttcatc aaacctaata tccatgagtt
    14701 ttcagatatc atccatcaaa aaattactca aaaaagcgac ataaaacacc atctaaaaac
    14761 aaatccacta tttaaagata tcgatgttat catgatttct tgcggctcgt atggtgcagt
    14821 agtcaaagta aaagaagaat tttggaatgt tgagatccct aagattccta tcattaatac
    14881 aacaggctct ggggatgcca ctgttggagg ttttgctcat tcattagaac agaattatcc
    14941 tttaaaagaa tgtgtaaggt acgcaatggc ttgcggcatg tcgaatgcac agcaatcaga
    15001 agtcgggaaa gttattccag ctgatgtaaa aaatttagca aaaagcatta aaatcacaga
    15061 aatgaaataa atagtacaaa gtaaataatt gtttatatga aatgagacac aaagagactg
    15121 tgacaaaaaa cgtgtgtacc gacccccaaa agttagattt tttggtctaa cttttgggag
    15181 tcgatacatt gtcacagtcc ttttttcgaa taaacggtgt tctcctgcca actcctcggt
    15241 cacaagtcac aatattcaac aaacatgaga agctattttt tgaaattgct ccttgtgact
    15301 cagagttaaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gcttgcatct
    15361 acgctaatga gtccaaccaa gtcaaaaatg aggagctttt ttggctgtcc ttattacttg
    15421 atgcagggca gttttttcat aatctctttt agattaaatc cttttggtta cacaaggttc
    15481 tcttcaaaga atgttgccat tttttcaaat ggaatgatgt ctgtctggtc gtatagatct
    15541 gtatgggatg ctcctggaat gatcatgagt tccttgttgt caccttttag ttctttgaaa
    15601 gcgtcctccc caaagtaacg tgaatgagct ttttccccgt gaatgactaa gactgcatga
    15661 cggatttctg gaatataatg caatagtttt gcattcataa gtgacaaccc tgatgtccta
    15721 ttccaaccat cattggagtt taaagaacgg acatgatatc cgcggtctgt tttataatac
    15781 gcatgatagt ctttgacaaa ttgaggagca tcttcaggca acggatcaac cacacctcca
    15841 gcacgtgcgt agtccccttg tttgaaatca atgatgcgtt gtgcatttaa ttcttcacgc
    15901 atttcatatc gggcattttc atccataaca tcgaagtagc catttgctgt tactcgagtc
    15961 atgtcataca ttgtggaaac aactgttgct ttgacccgcg tatcgatagc tgctgcattc
    16021 aatgccatac ctccccatcc acaaatacca atgattccga ttctctcagg gtttacattt
    16081 ttttgtacag ataaaaagtc tactgccgct tggaaatctt ccgtattgat atcaggcgaa
    16141 gcaacatatc gaggtactcc tccgctttct cctgtaaacg atggatcaaa cgcaatcgtt
    16201 aaaaagcctc tttcagccat cgtctgcgca tatagaccag acgattgttc cttcactgca
    16261 ccaaatggcc cgctcacagc gattgccggc aatttctttt cacttacttc ttttggttca
    16321 tataaatcag cgactaatgt gataccaaaa cgattcggga agctcacctt tcgatgattg
    16381 acttgattgc tttttggaaa tactttgtcc cattcttgtg tgatgtttaa ttctttattc
    16441 atattctaga ctctcctttg atcaaaattg acatttgttt ttgaatgtat tattatgata
    16501 aaacttaaac ttgactttag gtcaaggtaa aaattttatt ttaggaggat ttttttatgt
    16561 attccattgg acaagtgtct gaaatgtttg gattgcctgt ttctacttta cgttattacg
    16621 acaaagaggg attatttcct gatatccagc gtgtttctgg tattagaaaa ttttcagaga
    16681 atgagttaga ggctttaaga gtcattgact gtttgaaaaa atctggttta gaaataaaag
    16741 acattaagca attcatggaa tggagcaaag aaggcagtaa gacatttgag gttcgtaaag
    16801 aactatttga aaaacaaaaa gaagtagtag aaaaagaaat agcaaaatta gaaagagtac
    16861 tcgctatgct aaactacaaa agctggtact atgaagaagc aataaaagcc ggcaatgaag
    16921 aagccgtatt gacaatgatt cctgatgatt taccccaaca tgtaaaagaa gcttatgtac
    16981 attctcattg aacaaacgga taactcactc tccttctcta tgaacttcat gaaaacatga
    17041 ataaacacct ctaactgatt gttagaagtg tttatttttt cactcaagta tcctgcactt
    17101 acaaaaatat cctttttacc gccatcctaa ttctggagcg atatctttta cgattgtttc
    17161 aagcaagtgc atgttatatt ctacccccaa agtattagga atcgtgacaa gtactgtgtc
    17221 tgcttctttg attgcttcat cttcagctag ctccttgatt aattgatcag gttcaccagc
    17281 aaaagttttt ccaaaaatcg ttgggtttcg gtgataagca agatagccga cagcatcttg
    17341 ttgcggacta ccgtcttgtc caaataagcg tcgatccaaa tcagtggtga ttggctgaat
    17401 cgagcgagta actaatgttc ttggttcaaa atcatgacct gcttgcttcc acgcttcttt
    17461 atacgcacgt aattggtttg cttgttggat atggaatggc tcgtgggatt cgtaattgac
    17521 taatgtagat gattgcagat tcattccatg ttgtgctgcc cagattgccg tttgctgcga
    17581 accagctccc caccagattc tttgtcgtaa tccttctgaa taaggttcta tccgtaattt
    17641 tcctgatcct tgtgggaaca ttggttgagg atttggttct tcaaatggtt ttccttcaag
    17701 taattccaag aattcaaggg tgcgttcccg tgttacctct ttgatttctt cttcactgta
    17761 atcataacca aagtatttcc agccatctag aacttgttca ggtgaaccac gccccactcc
    17821 tagttgcact cttccttgcg tgatgatatc tgtcaatccg gcattttctg ccatataata
    17881 agggttttca tatcgcatat caataagtcc tgtaccgatt tcaatcttac ttgtctttgc
    17941 gccgattgcc gctagcaaag gaaacggcga cccaatctga ggggcaaaat gatgtacgcg
    18001 gaaaaacgct ccgtccactc cgattttttc tgcttccacc gctaaatcaa tcgattgcaa
    18061 ataagcatct tgagctgatt tcacaagcga tccttgctcc atccaatgtc caaagcttaa
    18121 aaagccgatt tttttcaagg taatgacctc ctttaaacct acaaaaatgg aaggtttttt
    18181 atttttaata tgaactgatt ctactacttt ttttcttaaa agacgaataa ttgttctcgc
    18241 ctcacaatca agaaatgtat cggcttacta cacccctact ttcttcatca tctgccgtgt
    18301 cttaatctct tttaacagtc tgttacatcg ttgatttttg actcaaaatg attgtttcta
    18361 acatgtattt atggtaaaaa agacaagatt agttctttgt acacatcgat ttatacgctg
    18421 aacaaaaaga aatatataaa ctaagaaacc cattgttttt tcgctccatc acatagagtt
    18481 tactttagat aaacagtgac atttactaat ccataacgga ggaaatcaaa tgaaaaaaat
    18541 ctatttctta tgtacgggca attcatgccg cagtcaaata gcagaaggct acgcgcgtaa
    18601 atacttgtct ccttcaatgt ttgagataag
//
LOCUS       DACCQP010000047        18504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCQP010000047 DACCQP010000000
VERSION     DACCQP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.049"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IVO87_001820"
     CDS             99..746
                     /locus_tag="IVO87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7449995.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVO87_001821"
     CDS             1139..3784
                     /locus_tag="IVO87_001821"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7449996.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVO87_001822"
     CDS             4094..5407
                     /locus_tag="IVO87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7449997.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVO87_001823"
     CDS             5397..6050
                     /locus_tag="IVO87_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7449998.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IVO87_001824"
     CDS             6105..6797
                     /locus_tag="IVO87_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7449999.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IVO87_001825"
     CDS             6947..7147
                     /locus_tag="IVO87_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7450000.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IVO87_001826"
     CDS             complement(7401..9080)
                     /locus_tag="IVO87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7450001.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IVO87_001827"
     CDS             complement(9082..9294)
                     /locus_tag="IVO87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7450002.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IVO87_001828"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IVO87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7450003.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IVO87_001829"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IVO87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7450004.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IVO87_001830"
     CDS             13269..13466
                     /locus_tag="IVO87_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450005.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IVO87_001831"
     CDS             13619..14104
                     /locus_tag="IVO87_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7450006.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVO87_001832"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVO87_001832"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7450007.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IVO87_001833"
     CDS             14981..15508
                     /locus_tag="IVO87_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7450008.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IVO87_001834"
     CDS             15599..16336
                     /locus_tag="IVO87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7450009.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IVO87_001835"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IVO87_001835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7450010.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IVO87_001836"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IVO87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7450011.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACCQP010000048        18117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCQP010000048 DACCQP010000000
VERSION     DACCQP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.050"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IVO87_001837"
     CDS             complement(24..722)
                     /locus_tag="IVO87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7450012.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IVO87_001838"
     CDS             complement(715..2067)
                     /locus_tag="IVO87_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7450013.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IVO87_001839"
     CDS             complement(2290..3690)
                     /locus_tag="IVO87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7450014.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IVO87_001840"
     CDS             3851..4588
                     /locus_tag="IVO87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7450015.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IVO87_001841"
     CDS             4713..5192
                     /locus_tag="IVO87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7450016.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IVO87_001842"
     CDS             5189..5956
                     /locus_tag="IVO87_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450017.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IVO87_001843"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IVO87_001843"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7450018.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IVO87_001844"
     CDS             complement(6859..7461)
                     /locus_tag="IVO87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7450019.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IVO87_001845"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IVO87_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IVO87_001846"
     CDS             8142..8726
                     /locus_tag="IVO87_001846"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7450020.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IVO87_001847"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IVO87_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7450021.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IVO87_001848"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IVO87_001848"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7450022.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IVO87_001849"
     CDS             10627..10806
                     /locus_tag="IVO87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450023.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IVO87_001850"
     CDS             10828..11853
                     /locus_tag="IVO87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7450024.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IVO87_001851"
     CDS             12028..13272
                     /locus_tag="IVO87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7450025.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IVO87_001852"
     CDS             13425..14372
                     /locus_tag="IVO87_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7450026.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IVO87_001853"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IVO87_001853"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7450027.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IVO87_001854"
     CDS             complement(15274..15516)
                     /locus_tag="IVO87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450028.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IVO87_001855"
     tRNA            15637..15709
                     /locus_tag="IVO87_001855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IVO87_001856"
     CDS             complement(15828..16466)
                     /locus_tag="IVO87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7450029.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IVO87_001857"
     CDS             complement(16589..17221)
                     /locus_tag="IVO87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7450030.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IVO87_001858"
     CDS             17572..17871
                     /locus_tag="IVO87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7450031.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6210 a         3162 c         3570 g         5175 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattgg
//
LOCUS       DACCQP010000049        18101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCQP010000049 DACCQP010000000
VERSION     DACCQP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.051"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1013)
                     /locus_tag="IVO87_001859"
     CDS             complement(<1..1013)
                     /locus_tag="IVO87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7450032.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(1094..1696)
                     /locus_tag="IVO87_001860"
     CDS             complement(1094..1696)
                     /locus_tag="IVO87_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7450033.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            1881..2582
                     /locus_tag="IVO87_001861"
     CDS             1881..2582
                     /locus_tag="IVO87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450034.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            2592..5303
                     /locus_tag="IVO87_001862"
     CDS             2592..5303
                     /locus_tag="IVO87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7450035.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            5700..7328
                     /gene="treC"
                     /locus_tag="IVO87_001863"
     CDS             5700..7328
                     /gene="treC"
                     /locus_tag="IVO87_001863"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7450036.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(7388..8569)
                     /locus_tag="IVO87_001864"
     CDS             complement(7388..8569)
                     /locus_tag="IVO87_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7450037.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            8821..10347
                     /locus_tag="IVO87_001865"
     CDS             8821..10347
                     /locus_tag="IVO87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7450038.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(10416..11144)
                     /locus_tag="IVO87_001866"
     CDS             complement(10416..11144)
                     /locus_tag="IVO87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450039.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(11157..12437)
                     /locus_tag="IVO87_001867"
     CDS             complement(11157..12437)
                     /locus_tag="IVO87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7450040.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(12578..13180)
                     /locus_tag="IVO87_001868"
     CDS             complement(12578..13180)
                     /locus_tag="IVO87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7450041.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            13426..13632
                     /locus_tag="IVO87_001869"
     CDS             13426..13632
                     /locus_tag="IVO87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450042.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            13945..14664
                     /locus_tag="IVO87_001870"
     CDS             13945..14664
                     /locus_tag="IVO87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7450043.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(14745..15428)
                     /locus_tag="IVO87_001871"
     CDS             complement(14745..15428)
                     /locus_tag="IVO87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7450044.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            15660..17846
                     /locus_tag="IVO87_001872"
     CDS             15660..17846
                     /locus_tag="IVO87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7450045.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         6335 a         3141 c         3527 g         5098 t
ORIGIN      
        1 tatttcaagg gtgcagttga aaatcttttt tctaatcgta caggtaatct tttttcttcc
       61 cattcttttt ctgggaattg gatccgatct aacaataatt tggcagatgt ttgtcccaat
      121 tcgaagatcg gttgtgcgat cgttgtcagt ttcggcttga tatactcgca catatctata
      181 ttatcatagc caataatgct gtagtcttca ggaatgcttt ttccagattc ctctaatccg
      241 cgatataatc caaaagctaa ttcatcattt aaagcaaaca caccagttgc agcagaatca
      301 agaagttctg ctgtgatttg gtagccacct tgttttgaga actgtgtagg taagaggatc
      361 agttggtcgt gagagatttg gtaaacatca agagcagatt taaacccctc aatccgagcg
      421 tggacatttt ccggcggatt ttcaggatag acaagggcga tcgtttgatg acctagacta
      481 agcaagtgca tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta
      541 aatccttcag attttttctg atccaaaaca atgaagggcc ggccttgttt tttcaaattt
      601 tcgttgattg catctgtaga gacagcagaa gtcgcgataa tgaatccatc cacacctcga
      661 cgtgtgagct cggctagata ttcgatttct ttttgatgat cagaatcagc attacataat
      721 atcgtgacga aattctgctt atacaagata tcctcaattc ctcgcatcag tgtactgaag
      781 aatggattcg tgatatcagg aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt
      841 tgtctagcaa aatagtcagg ctgatagcct aattcttctt tggcagctaa aactttctga
      901 acagttttcg gactgaattt ctccgtattt ccatttaata tcaaggagac agttgtgatt
      961 gaaacgccgg aaagtttagc gacttctttg attgtgatct tttttttcac catgttcata
     1021 ctcctatcta aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa atgaaaatca
     1081 cgtgtgccag tacttatttg ctttgttgga tagcttcgat tatttgtttc gttgagcgta
     1141 ctcgtgcagt tttcggaaat atctttttta tgaacaattg atgggattct ttgtcacgat
     1201 cactgcaagc atcttcagca agtacaacat gatacccatg ttggaaggca tctaatgcag
     1261 tagcatatac accgtttgtt gtcgctacac cgcttaaaac gatagtttcg attccacgcc
     1321 ggcgaagttg aagatcaaga gaagtaccaa aaaaggcact tggattgtat tttgtgatag
     1381 tcaacatatt gtttgtatct ttcaaggaat ccttcaacaa caagtcagtg aattctgccg
     1441 gagcagacaa agcagatatt cctccagcat ctgtctgatg acttagataa gagaaagtag
     1501 tgatgtctac attcactaaa actttcaagg cagcggtacg tttcaatgtt tttgctaaca
     1561 aatcattgtt tgtcagaata gattgtgcag tgtgtggaga gagtgtaccg ttatttacga
     1621 tccctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
     1681 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
     1741 cataaatcgt ttatttgtca actaaataaa ggattccttt ctttagtcta gagaaattca
     1801 gtggtaaaat aaataagtat gagaaaacgc attcgatgca tagatatagc gagaaatatc
     1861 gtgaaaatgg aggaatcaga gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca
     1921 aaatgggaga aacgaccatt acagcaaatg acgatatttc tttttcaatt gaaaaaggcg
     1981 aattagtgat tatacttggt ccaagtggag caggaaagtc aacgattctt aatattttag
     2041 gtgggatgga tacacctgat gaaggtcaga tcattattga tgacacagat attgcgcaat
     2101 tttcagataa acagcttact gcttaccgac gaacagatgt agggttcgtt ttccaattct
     2161 acaatctcgt tcctaatcta acagcaaaag aaaatgtgga attggcaacg gaagtttctc
     2221 ctgatgcgct tgatcctgta gaggtattga ggcaagtggg cctagctcat cgattgaata
     2281 actttccgtc ccaattatca ggaggcgaac agcagagggt ttcaattgct cgtgcactag
     2341 ctaaaaatcc caaacttctt ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg
     2401 gcaagcaagt attgaaatta ttgcaaaacg ctagcagaca acacggcaat accgttttga
     2461 tcattacaca taattctgct ttagcgccca tcgctgatag agtcattcac attaatgatg
     2521 ctaaagtaag gtctgttgaa ttaaatgatc atccttcatc cattgatgag attgtatggt
     2581 agggggattt tatgaaaaca agaacatatc ttaaagcagg ttttcgcgaa attcggcaat
     2641 ctaaaggacg attcattgca atcatcctta ttgtcatgct aggaacactt ttatttgtag
     2701 gtgtcaaaac tgctggacct gtcatgcaaa aaacgatgga ccactatgtt ggtacagcag
     2761 gactttctga tttgcaaatc gtttctactg gtggcttgac acaaaaagat attgctcaag
     2821 cagaaaagat cccagatgca aaagtagaaa caggaaaaca gatctattat tccaatactg
     2881 gtaaaaacga agtgattcaa atattttctt ataacaaaaa taacaagcag aacaagctac
     2941 aattaactga tggaaaactc ccggaaaaac caaaccagct tgtattggat gaaaaagcgg
     3001 aagatgaagg gtataaaatc ggtgatattt atcagattga ttcagatgaa ttgaaagaaa
     3061 aagagtatac catcacgggc tttgtccgtt ctccattgtt catcaacaat ttggagagag
     3121 gatacgcgaa cgtcggaaat ggtagcgtcg attattttgt ctatttacct gaatccgttt
     3181 ttaaaacaga tatttattct gtcttatatc ttgattttac aaacgtaaag aatttgaata
     3241 cgtacagcaa agcttacaaa gataaaatgg aacacaatca agagaaggca gaaaagtatt
     3301 tacaagatcg ccctgaagaa cgcttagaag agctgaaaaa atcggcaaac gaagaattag
     3361 aaccagcaca gcaaaaaata acagaaggca aagcacaaat agcacaggga agaacacagt
     3421 tagaagatgc taaaaaacaa ttagcgcaac agtcagcagc tatccagcag cttcctgaag
     3481 cgcaacgaac aatcgctgtt caaacattat ctcaacaaga agctcaactt gctgaacaag
     3541 aaaggcaatt agttgagaaa gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa
     3601 atgcagaaaa aattgaaaat ttaactaaac catcatacct atacaatgaa cgttcagaaa
     3661 atgtcggttt tcaagaattt ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt
     3721 ttcctgtttt ctttttcttt attgccgcat tgatcacctt tacgacaatg acacgtatgg
     3781 tagaagaaaa ccgcagggaa atcgggacat taaaggcgtt aggttatacg aaagttgaaa
     3841 tcgcaaaaaa atatgcaatc tatgcaagtc tagcctcagg gataggcatt gttttaggaa
     3901 cgatattagg aacgaatctt ttgccaagga tcatttttga gctgtctaac gagcggtatg
     3961 atattgggag tgcgcttatt ttttatgact ggccgccgat tttacaagca gctgccgcat
     4021 tcttcattgc cgcatttggc gctgcaatga tcgttttatt caaagattta agagaacggc
     4081 ctgctgcatt gcttcaaccg aaagcaccga agccagggaa acgcattttt cttgaataca
     4141 tcactccgct gtggtcacgc cttagcttta atcaaaaaat cagttaccga aatctcttcc
     4201 gatataagtc aagaatgctt atggcgatta tcggaattgc tggatgtgcc gggctgatgg
     4261 ttgcaggcgt gggattgaaa gattctctaa gttctgtctc agctaaacaa tttggaccga
     4321 tcattgacta tcaagcaatc gttacattgg attcatccac ggaagatagt cgtcaaaaag
     4381 cagcggatat gctgcagaag caagaaaaag taaatgagat cctagctatt catactcaaa
     4441 ccgtagaatt gagaaaaaaa ggacatgctt cgcaaagtct gacagtgatc gtaccaagta
     4501 aaacagctga tttggatcgt tttatccatt taactgatct tgaagagaac ccacgctcct
     4561 tgcctaaaac aggaatcgct attacgcaaa aagcagcaga actatttgac ttatctacag
     4621 gagatcatat ttcgctttat gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa
     4681 tcgtgaaaaa ctatctaggt agctttattt atatgtcccc tgaatattat gcttcagtta
     4741 ctgatcaatc gatgatggat aatgcatttt tgctgcgtac agataagatg acaacaaaag
     4801 aagaagaaat gctttccgag aaactgcttg ccacagacgc tgtgacaaat acgtctttca
     4861 tgtccaaaca aatcgaaacg caagaagaat caatggcgaa cttagactcg gttgtcttaa
     4921 tctttgtcgt attatcagga ctgctggctt ttatcgtctt gtataatcta actaatatca
     4981 atatttccga gcgtgtacgc gaattatcta caataaaagt ccttggattt tttgataaag
     5041 aagtcacgat gtacattgtt cgagaaaata tcatctttac tcttttagga atcatcggag
     5101 gatttggtgt aggatatata ttgactgact tcatcttgca acaagcgtct atggagaatg
     5161 tcatctttcc attagtgatc acatggtggt cttatgtctt atcagcagga ttaactatcg
     5221 tatttacggt tattgtgatg attgttaccc atttcaaatt aaagcatatc gatatgattg
     5281 atgcgttgaa atcgaatgaa taaaaacaga ggttgtgaaa tcagcgtttt gagctaataa
     5341 cgagggtcag ctgctaattg aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat
     5401 cgagcaaaag gaatactcaa ataaaattag ctcctcatgt tttacatttg agaagactga
     5461 ttgccgtaga taaaagcttt tgagcaccat ttatagaaga aacaagaata ttttgctatt
     5521 ttaaacaaga ggatagacaa atacatgtct gatctcttgt tttttattgg gcaacagacc
     5581 tcttagaatg tataaagata aattttacaa acgctttctg ttcagttata ataataaatg
     5641 ataacttgta tatataagtt gcggtaggta ataaatcagt tgaatggagg taaatatcca
     5701 tgacatttaa agacaaagtc atttaccaaa tctatcccaa atcttacaaa gacagtaatg
     5761 gtgatggaat tggagattta aaaggaatca aagaaaaact cccgtatctg aatgatctag
     5821 gcatcgacat gatttggcta aaccctattt atccaagccc tcaaaaagac aacggttatg
     5881 atatctctga ttacacagcc atcgatccta tctttggtac gatggaggaa ttcgaacagc
     5941 taatagccga agcaaagaaa taccggatag aaatcatgct cgatatggtg ctgaatcacg
     6001 tttcgacaga acatgaatgg ttcaaaaaag cactagcagg ggaccagcat tatcaggact
     6061 tcttcatttt aagagaagag ccaacggatt gggtatcaaa atttggtgga agtgcttggt
     6121 cgccatttgg agatacgggg aaatattatt tgcacttata tgatcggaca caagctgact
     6181 tgaactggcg gaaccaagca gtaagagagg aactattcaa agttgttcgt ttttggatgg
     6241 aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat
     6301 tgaaaaataa caaagaaaat gatggcaaac ctgaatatac ggacaaacca atcacccata
     6361 cgtatctaaa agaattgaac caagcaacgt ttgggacgga ttccactatc atcacagtcg
     6421 gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta tacagaacct agtagagaag
     6481 aattatcaat ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat gggaataaat
     6541 ggtctactcc tccatttgat tttgcagaat taaagcgact atttcataca tggggagaaa
     6601 agatgagtga gggtaacggc tggaatgctc tattttttaa taatcatgat cagccacgtg
     6661 cgctcaatcg atttgtagct atcgatcagt atcgaaaaca aggagcagag atgctagcaa
     6721 ccatgatcca tttgaaccgt gggacgccct atatttatat gggagaagaa atcggcatga
     6781 tcgacccaga ctattcatcg atttccgact atattgacgt agaaagtttg aatgcttata
     6841 agttactcct tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat
     6901 ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac agaacaagca ggatttacta
     6961 cagggaaacc ttggttaaaa gttggaggca agctagaaga gattaatgta gaaaaagaac
     7021 tatcttctga agattctatt ttgtcctatt acaagaaatt gattcggctg agaaaaacat
     7081 atccaatcgt tgcacaagga gattatcatg cttatgaagc aaatcatccg gaagtttatg
     7141 gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc tatgataaag
     7201 atacaaggat caccatccca gaaggattca cagaagccaa tattcttatc gataattatt
     7261 ccgatactgt tcttcatcaa agtctggagc taaaaccata ccaggcaatt gctttgctga
     7321 aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt
     7381 ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga gttttcatac tctcctttat
     7441 cgccataaaa tttattgtag cgttgtttga atttacggaa aataaaagta acgagtaatt
     7501 tgactaacgt atagatagga ataccaaaaa taacgcccac aattcccatt agatcaccca
     7561 tgacaagtaa aacgattaag atcgtaatcg gatggatctg taaacgatca cccattactc
     7621 gaggaatcac caagtcaccg tggagaagtt gtacggcaaa ccagacaatg acaaatttga
     7681 cagccataaa agtagagtct tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg
     7741 gcccaatata tggaatgata cataacaacc cagcagaaat agctaaaaca ctagcaaatt
     7801 ctaatccgat cagtaagaaa caaacccagt agactgctcc aaggataaat gaagcaatga
     7861 tttgcccctt tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa tttttaaaat
     7921 ctgtacgaaa tgctggtggg acaatcgcta aaactgactg ataaaatttt tgaggatctc
     7981 tcaaaagaaa aaaagcgaca attggaccag taaataaagt aataaaaata gctgtaacag
     8041 ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca ataatttcca ataaatccag
     8101 ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa tcggcaaaag
     8161 gtgttttctc taaaaattgt cggagactct cttgaaaatc ttgaaacaaa tctggcaaac
     8221 tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaac aagccatacg cttccaagcc
     8281 ccaataaaaa taaaataatc acataaacga taatgactga caagttcctc gaaaggtgct
     8341 tgctcaaacg tttgatgact ggttccaaaa gataatacaa gatcagtgca aacaataatg
     8401 ggggcaagat agtagcgata atgattccga caggcttgaa tataaacgag atctgatgga
     8461 aaaaataaat gatcagcgca agtaagataa ctattcctaa aacatagtaa gaggtttttc
     8521 ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc ttttttcact ctttttcctc
     8581 ctttatctat aacttcatta tagacaaaag cgggtataaa aggaaaatga tagaggttga
     8641 ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat aagaaaacgc
     8701 ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt aactattacg
     8761 ttttgtaaaa ggcgaatcgg aacattcaaa tgaaagaaga tgagaagagg agtgtcataa
     8821 atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt aactgcttgc
     8881 ggcggggcaa gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt
     8941 tatcctatgt atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg
     9001 attccagcag ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc
     9061 caagatacag atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc
     9121 caaaaaacat tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta
     9181 cttccgggaa gtgaagaagg acatgatcat gaccatgaac acggggaaga aggccataca
     9241 catgagctag acccacatgt atggctagct ccaagtttag caatcaagca ggtagcaaac
     9301 atacgtgatc agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca
     9361 gcagcctata ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa
     9421 gcaaaacaaa gatcttttgt cacgcaacat gctgcattca actatctggc attagaatat
     9481 ggattgaatc aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga
     9541 attgctgaac tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa
     9601 aacgccaaag atagtattgc cagaacgtta gcaaacgaag caggtgtatc tttagaagta
     9661 ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct
     9721 atcatggaag caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgctagaa
     9781 gatactttga cccccaaaaa agaaaagagc gtctacaacg gttattttga agacgaggat
     9841 gtacaagatc gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatgta
     9901 gaagatggga cttttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca
     9961 gcacaagaat ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag
    10021 atcagtgaga aagagatggc atttacgttt aaggatggaa caacgaaacg ttcaaattat
    10081 cgttatgtag ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta
    10141 ttcgaagcag aagataagga aagtggcgct tttagatata ttcaattccg tgatcacacg
    10201 attgcaccgg ctgactccga acattttcat ttatatgcag gaaatgaaag tcaagaagca
    10261 ttgctgaaag aaatggagaa ttggccaaca ttttatccag atacaatgga tggacgtgaa
    10321 attgcacagg aaatgctttt gcactgatag gcaaaaaaga accgaagggt ttggatcggc
    10381 tagtcattta gtcggtccaa actattcggt tctttttatg aaatcaattt ctttaatcca
    10441 cggatagagg ctgggatggt ctcgccgttt gccaacgtga cttctctggt ttttttatta
    10501 atcgaccgca cattttgtgt attgataaca taggatttat gacaacggat aaaattccga
    10561 tgcattttct cgatttcttt gatcttctca tagaattgta attgggcatg agtcaaatgg
    10621 acttccagtt tatgtggtgc tcctagcgaa gtagaaaaat attggatgtc tttgatcggt
    10681 aaaaatttga tctcatcatt aaactctatt ttgaagatat ctttttggat ctgggacatt
    10741 tgaaaatctt cgaaaatttt gctcaggata tgctcgacat tttcttttaa atcataaaga
    10801 tcttctttga cgatataatc agtcggttcg atgttgcttt ttaagatttt catcgacatt
    10861 tccgtatggg aagtcacata gattattttt cctaagggat ctaattcacg gatcaattta
    10921 ccgatcgcta caccatccat ttcactgtct tctagttcga tatctaaaaa gtaaatacca
    10981 aaagaagaag ttctgatctt ggctgcattg agaagttctt ccggatcaga ggtagaaata
    11041 tggacataac tgtctaagtt ttcaatcatg attctatttt tgatcaccgt tgataaaact
    11101 tctaattgtt ttggatcgtc ttcacaaata taaatgggaa tcatatcatt actcctttat
    11161 tttatttgta tataaatcgt ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa
    11221 agataaggat tggtctttgt caattcatcc aagataaaca agccaaggcc ttcgtttttt
    11281 ggatttgttt ttgttgagaa ccctttttgt ttcaactggt ttaacggcaa tgtatttgtt
    11341 gttgtattga tcacacgaat catcacggaa tgattcttct tgagtatggt caattcgatt
    11401 tttttcttct ttgcagcact agctccttca atcgcattgt ccaagataat accaagcata
    11461 cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa atgaagctgg
    11521 acgaccaacc ctttttcctg tgccatcatg agttttaatg acagtaaact acggatctca
    11581 ggaactttca tattatccaa catacttaga ttggccggaa aaaggttcat catctggcgt
    11641 gtaggtaata tcgtttgagc ataataagac cgtaatcctt ctaagtcgcc ttctgaaata
    11701 tagctgtgaa gagaaaaaag cagattttgg taatcatgtt tgaattctct caagtcttga
    11761 tagttttttt ctagatcttt gatataatcc tgttgcatcc tgacttgttg ctctaataac
    11821 tgatcagcta atttcttatt agagattttc caataataaa ccaagatgat caggccaaca
    11881 cacagatata gaatgaaaaa actcgtattc aaagctaaga catcaggtgt ttcaccagaa
    11941 aaacgggtat aaaaaataat gatatagtat gtaaataata tcaatgtccc gatcgtaccg
    12001 atcatacggc ggtccacagt gtgaaaccaa gtctctaacg attttttaaa acaaaaagct
    12061 aataaagcag ataaaaaagc ggataaggca atgtgaaaat actgcaatga atctcctggt
    12121 tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa
    12181 agcgacgtca ataacccaaa aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag
    12241 aagaaaaaag taagcatcag cattcctata gcaattagag cagtaaaaat ccctaaaatc
    12301 gaatagaccc atccaatcaa aatacaagaa atcagaatga ctcccgcaaa cagatatttg
    12361 tatcggatgt gatggtcaat caataagata caaattgtgt aaatcaaata ttgcaacgct
    12421 atggaccata aaaacataaa gtacagctcc cattgatttt taccaaatta tagcacaatt
    12481 agcgtattat ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca
    12541 gccagataca aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa
    12601 agattcctcc aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa
    12661 caatcccaag tagcaaatag gtagaaaagt aagaagctga gaaaaataaa gtaatgagag
    12721 aaaaaattag aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa
    12781 tgggattgat tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta
    12841 acaaaaagac catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa
    12901 cgccccataa ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc
    12961 cataagcata tctgcggatg catatataag aaagaaaggt gattcctgtt tccgcccatg
    13021 agcctgtcac cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa
    13081 cttctaaggc aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga
    13141 ataaggcttc taataatttg ttcacaactt tatctttcat ctcatacgta cctctaccac
    13201 aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat
    13261 caaaaccgtc atgatttcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt
    13321 gacagttttg atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat
    13381 gttaaaaaaa ttactgacct tataaaaggt gatggaggga ttcttatgga aaatgaacaa
    13441 ttgtttgaag tcggcgaaac atatgcattt aaatctgatt tatttgaatc accaataaaa
    13501 ggaaaaatag aaaaaatcta tgaaaatagt gtgatgatac gtgtcatgaa atgcgcagta
    13561 gaagacaatg ataccgctca taatctgcac tggcagttag ttgtacgcaa atcaagtaca
    13621 atagaagaat aaaaacagat aaaatgttga gagcataaag aggttgtgag tctccgttta
    13681 gctctgagtt acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcca
    13741 cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga
    13801 cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt
    13861 agcttttaag gagcagataa agtgaaggct tttatttcta taaatgaagt agtaaaatga
    13921 agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc
    13981 ggtgcaggga aaagcacact gactgaaatg cttgcccaag atttagaaac aaaacctttt
    14041 tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat
    14101 acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc
    14161 agccatgatg acaatgtact agaccgctcc atctacgaag atagtatgct gtttcatttg
    14221 aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa
    14281 acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat
    14341 attcgtgtat cttttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa
    14401 cagctagaac aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa
    14461 gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat
    14521 ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag
    14581 aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca
    14641 aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt
    14701 tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga
    14761 ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa
    14821 atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata
    14881 gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct
    14941 ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt
    15001 gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat
    15061 gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta
    15121 acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct
    15181 ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg
    15241 gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat
    15301 agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata agtataactg
    15361 tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa
    15421 gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac
    15481 gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt
    15541 ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg
    15601 aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa atcaatcagt
    15661 tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag aacaagtata
    15721 aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat gaagcggctt
    15781 atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt gaaggaaaca
    15841 gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa gaacaaaaag
    15901 cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct gggagcggca
    15961 aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa gtagacgaag
    16021 aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa aaacgagtag
    16081 ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt atctttttta
    16141 ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga atacaaggag
    16201 acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg atacgtaaat
    16261 ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg ttagactatt
    16321 ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa aaatatgatt
    16381 ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg ctcacggaac
    16441 tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc caaaatctga
    16501 aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat gattatctat
    16561 tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa cggatgacaa
    16621 aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc tatggattca
    16681 gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc aaaactcttt
    16741 tcttattaac taattatcgt tctcgggcag ctattgtcca aggagcaaat agattgatca
    16801 gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa gaagcaggtg
    16861 ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta atcaaacaaa
    16921 ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta taccgatcag
    16981 cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct tttgtactgg
    17041 aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat ttctatcaaa
    17101 aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc taccagcaat
    17161 tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat caatactttt
    17221 ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc cccaatttaa
    17281 aatcaaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca gaccagcatc
    17341 aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct aaggaaatcg
    17401 accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact ttcagaagac
    17461 tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc cgcttgattg
    17521 cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa agtaaaggtc
    17581 ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct tataacggag
    17641 cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg acgacgatag
    17701 aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt ctgtatctat
    17761 gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc aaagaaaccg
    17821 ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt ggtcagtttc
    17881 aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat ttcagcttat
    17941 tttcgaagaa gctatttctg gaacaccgtt tattcggaaa aaggaggtgt gacaagaatt
    18001 ttgtcacacc tcccttattt attttgaaaa tgagaatact attatcaaaa tagagaatat
    18061 atagaataat tctcattatt caaacgtaaa taatcagtta t
//
LOCUS       DACCQP010000050        18054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCQP010000050 DACCQP010000000
VERSION     DACCQP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.052"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IVO87_001873"
     CDS             214..498
                     /locus_tag="IVO87_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7450046.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IVO87_001874"
     CDS             complement(779..1198)
                     /locus_tag="IVO87_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7450047.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IVO87_001875"
     CDS             complement(1200..1784)
                     /locus_tag="IVO87_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7450048.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IVO87_001876"
     CDS             complement(1831..3306)
                     /locus_tag="IVO87_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7450049.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IVO87_001877"
     CDS             3487..3798
                     /locus_tag="IVO87_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450050.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IVO87_001878"
     CDS             complement(3913..6249)
                     /locus_tag="IVO87_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7450051.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IVO87_001879"
     CDS             6391..7059
                     /locus_tag="IVO87_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450052.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IVO87_001880"
     CDS             7062..8105
                     /locus_tag="IVO87_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7450053.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IVO87_001881"
     CDS             complement(8342..9223)
                     /locus_tag="IVO87_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7450054.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IVO87_001882"
     CDS             9382..10110
                     /locus_tag="IVO87_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7450055.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IVO87_001883"
     CDS             10266..10583
                     /locus_tag="IVO87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7450056.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IVO87_001884"
     CDS             10576..11184
                     /locus_tag="IVO87_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7450057.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IVO87_001885"
     CDS             11200..12030
                     /locus_tag="IVO87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7450058.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IVO87_001886"
     CDS             12168..12680
                     /locus_tag="IVO87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7450059.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IVO87_001887"
     CDS             12706..13578
                     /locus_tag="IVO87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7450060.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IVO87_001888"
     CDS             13754..14116
                     /locus_tag="IVO87_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7450061.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IVO87_001889"
     CDS             14125..14877
                     /locus_tag="IVO87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450062.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IVO87_001890"
     CDS             14891..16693
                     /locus_tag="IVO87_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450063.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IVO87_001891"
     CDS             16970..18010
                     /locus_tag="IVO87_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7450064.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5664 a         3139 c         3728 g         5523 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aaactatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagact tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cctggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgata ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggtatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt ttta
//
LOCUS       DACCQP010000051        17872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCQP010000051 DACCQP010000000
VERSION     DACCQP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.053"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IVO87_001892"
     CDS             complement(51..1517)
                     /locus_tag="IVO87_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7450065.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IVO87_001893"
     CDS             1904..2500
                     /locus_tag="IVO87_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7450066.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IVO87_001894"
     CDS             complement(2558..3040)
                     /locus_tag="IVO87_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450067.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IVO87_001895"
     CDS             complement(3146..3343)
                     /locus_tag="IVO87_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450068.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IVO87_001896"
     CDS             3638..4396
                     /locus_tag="IVO87_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7450069.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IVO87_001897"
     CDS             4456..4884
                     /locus_tag="IVO87_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450070.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IVO87_001898"
     tRNA            4930..5002
                     /locus_tag="IVO87_001898"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IVO87_001899"
     CDS             complement(5152..5523)
                     /locus_tag="IVO87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7450071.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IVO87_001900"
     CDS             5738..5923
                     /locus_tag="IVO87_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450072.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IVO87_001901"
     CDS             complement(6006..6194)
                     /locus_tag="IVO87_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450073.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IVO87_001902"
     CDS             complement(6218..6481)
                     /locus_tag="IVO87_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450074.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IVO87_001903"
     CDS             6639..6896
                     /locus_tag="IVO87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7450075.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IVO87_001904"
     CDS             complement(6967..7158)
                     /locus_tag="IVO87_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450076.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IVO87_001905"
     CDS             complement(7299..7619)
                     /locus_tag="IVO87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450077.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IVO87_001906"
     CDS             complement(7642..8076)
                     /locus_tag="IVO87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7450078.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..9049
                     /locus_tag="IVO87_001907"
     CDS             8411..9049
                     /locus_tag="IVO87_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7450079.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9373..9456)
                     /locus_tag="IVO87_001908"
     tRNA            complement(9373..9456)
                     /locus_tag="IVO87_001908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9420..9422),aa:Leu,seq:cag)
     gene            9984..10484
                     /locus_tag="IVO87_001909"
     CDS             9984..10484
                     /locus_tag="IVO87_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450080.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10674..11018)
                     /locus_tag="IVO87_001910"
     CDS             complement(10674..11018)
                     /locus_tag="IVO87_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7450081.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11172..12092)
                     /locus_tag="IVO87_001911"
     CDS             complement(11172..12092)
                     /locus_tag="IVO87_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7450082.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12279..12770
                     /locus_tag="IVO87_001912"
     CDS             12279..12770
                     /locus_tag="IVO87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7450083.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12794..15169)
                     /locus_tag="IVO87_001913"
     CDS             complement(12794..15169)
                     /locus_tag="IVO87_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7450084.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQIAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15495..16121
                     /locus_tag="IVO87_001914"
     CDS             15495..16121
                     /locus_tag="IVO87_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7450085.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16144..16848
                     /locus_tag="IVO87_001915"
     CDS             16144..16848
                     /locus_tag="IVO87_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7450086.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17040..>17872
                     /locus_tag="IVO87_001916"
     CDS             17040..>17872
                     /locus_tag="IVO87_001916"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7450087.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5547 a         3484 c         3013 g         5828 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 cctaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa cctgttcaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac
     8761 acaagcagcg cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac
     8821 tccagcaagt acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta
     8881 cacagcaacg aacggacgtt acatcggccg ttaccaatta gattcttctt acttaaatgg
     8941 tgactactct gctgcaaacc aagaaagagt agcagaacaa tatgttactt cacgttatgg
     9001 ttcttgggaa gcggcgaaag cattctggga agcaaacggt tggtactaag attcattatt
     9061 tgaacgttta aacacattca aaatatagta agaggtcatg cgatttattc tttcgcatga
     9121 cctctttttg agtttaagtt tagtctttat tccttaacta tcgtttgcca gtatttcttg
     9181 tatcagaatc tataaatcta ctatttgaat caatctatta ggttttaaac tgtcccacct
     9241 tcacacaaat tttttttgat ttatttgaat ctggttccgt tgtttaaaat ttttcctata
     9301 ttcttttaga taatatgtta ttttctttct catcgctcca ggtgtacctt ctctttataa
     9361 aaaaacaaat tatgcggcta agaagactcg aacttccacg aggtcaccct cactagcgcc
     9421 tgaagctagc gcgtctgcca attccgccat agccgcatca catcttttag tttaggatta
     9481 catgtgtttt ttgtcaagaa catatttttg ttctttttca tttctgtacg ccctccctta
     9541 tgccatctac acaaataatt catccgagca actccatcat ctattgaggg ttggtgaaaa
     9601 aatctgtttt atccatatga tcgatttcaa tgttaaacga acgactctgg gattagtaac
     9661 tattttattt ttttcttcat ttcttttttc ctctttttct cgattcagct atcttctttc
     9721 atttggatat tgattatttc ttctgttgat tactatcctt ctggaattct agacgctgaa
     9781 gtaattcacg ttttctttct tctagagatc ataagtaacg aatgaacgaa cttcaaataa
     9841 tttttttatt gtaattcata tttgtattgt tcgatgattc ttaatacgat atgacacaat
     9901 aaataaaaat aggcatggct atttacaaca atagtgctat agtagaaaac aaatgaataa
     9961 ggttacttaa agaggtgaat acgatgaaac tgatcaaact atgggaagca gatttaaaga
    10021 aagcttatca gctacaaact agctttggac caaatgagag tggatttatc aacaatgctt
    10081 atggctatac atttgctgaa tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag
    10141 gaatcgatct tcctgagagt tacgttcccg caaccgtctt tattttagaa agtgatgaac
    10201 atgagtatgt aggaattttt aatttgcgcc ataaactgaa cgaaacatta agaaacggcg
    10261 ctggccatat tggttacggc atctccccta attatcgtaa aaaaggatat gccacacaag
    10321 gattagcatt agtactacaa gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg
    10381 taaataaaaa aaatcttggc agtcttaaag cacaacaaaa aaatggggct gcgatttatc
    10441 gtgaagatga gaaagaatac tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa
    10501 aaggaaaggg atttgaatgt cctttatcaa tagaacacat ttatttaatt aacttataat
    10561 atagcagaca aaattttata aacaaagaaa ccttttatct cttttcttct agaaaggata
    10621 aaaggctttt ttgtttttcc tatttgcttc tcatagctag tcagccaaga tttctatcgt
    10681 atccccaacg caatccgtgc ataacggctc atcttatctg tcgtccatgc tggataccag
    10741 actagtttga cttctgcttt cgttacttct ggcacttctt ttaatgcgtc atggatagat
    10801 tctgttaaaa tatctgctaa tggacagccc atagttgtta gagtcatctt aatgactgtt
    10861 tctccatttt caggattgaa ttctatatca taaatcaagc ctaaattgac gatgtcgatt
    10921 cctaattcag gatcaatcac catttccaag gcagcaagaa tacgttcttt gattagttct
    10981 atttcttgtc cagaccgttc ttggtttgct tcgctcatcg tacgacactc cttaacacga
    11041 tttttattta tcatactctt tttttagctg cttgtaaatg agaatttaga agtaagattc
    11101 tcgtttaaat ttataaaaat gttcttatta gatgtaatag attaaattta ttctaaaata
    11161 aacctgattc ctcatttttt ttgtgaacga ttttttattt ctggcaatac gccagttgtt
    11221 cgatacattt tttgttcgtt ttcatttgca aaccggccaa atactgaatg agttggtaaa
    11281 atattgataa atgcatcgtc gtcgatttca tgaacgatct gatcaagatc atacatttcg
    11341 tagcgggtga tgaccatcat gatcatgttc ccttgttttt ttgaatagcc accgacagac
    11401 ggcagtaatg tcattccacg taccatccgt tttgctagtt cttctgttac ctcttctggc
    11461 ttgacggtaa cgatcattgc tgttactttt tgatgactcg tgtggatggc atcaattact
    11521 tgagtcattg cataaatgga aataatcgta tacaaggcac tttcccagtt aaagaagaag
    11581 cctgcgatga ctacaatgat accatttaga atcagcatgt agtttccgac tgtttttcca
    11641 gttgtttgtg aaagtacaag agagatgata tccatcccac cagttgtaaa gcccattttt
    11701 aaagataatc ctacgccaag acctaataaa acaccgccta ctagggcatt catcaaaata
    11761 ttatcagtga tctgtccgac tggaagcagc atcgtcatca ccgatacaca tacaacattc
    11821 acaaaactaa ggatcgttgc tcgttttcct actttgagaa accctaatac aaacacaggg
    11881 atattcaaaa gaaaaataaa tgttcctgtg tctatcttga tatgaaacca ttcatataaa
    11941 agcgaggaaa tgatttgagc gatcccattc atccctgcag aaaagacatt ggctggaatc
    12001 aaaaaataat ttaaagcgac cgcagcggtc aatccggtta tcaaaataac cactattttc
    12061 tttaatcttt catttttatg atacgcagtc ataattttac tcacaagcac ctctccccta
    12121 ctttggtatt ccaaatgtaa gaataacagt ttatctatta aaataaaata cttcgaactc
    12181 aaatgagaag aattttagca aacaggtaag aatctgccct aatcctatga taagatagat
    12241 gtatggtaaa tatttatatg tatatcagga gggttcctat ggcaaaagat gctagcttcg
    12301 acattgtatc agaagtcaat acagaagaag taaaaaatgc gattcagcaa acaacaaaag
    12361 aattgtccaa tcgctttgac ttcaaggatt ctacagtaga aatcaaatta gagaacagca
    12421 atctagttgt attgagcgat gatgatttca aaatcgaaca gatcaaagat gtattatttg
    12481 gtaagctgat caaaagaaat gtgccaatca aaaatatcca tttttccgaa tcagaacacg
    12541 ctctaggtgg caaagctcga caaactgccg atttggtcaa cggaatcgat cgtgaaaacg
    12601 cgaagaaaat cactacagct atcaaaaatg aaaaactgaa agtaaaagca caaatccaag
    12661 aagatcagat tagagtgaca ggtaagaacc gtgatgatct acaagctgtg attgctttat
    12721 tacgtaaact tgacttacca atcgagttgc aattcactaa ctatcgttaa tcaaaaaacg
    12781 aggctgacgt ctgtcattcc tcgttttttt gatttactgc ttcttttgat aaccaaaaac
    12841 gctgtattcc ttcttccaat tccccaacac ttcgtacttc tcttgctgtt tgccaatgtt
    12901 gtgggaataa acgtcgataa gaaaaactag aaaatcgttg atctgccaat acaacgattc
    12961 cttgatccgc catgccacga atcactcgac caccagcttg caagactttg ttcattccag
    13021 gaagctgata agcgtaagca aatccaaggt gttccttctc atcataatat gatttgatca
    13081 gttcctgctc atgattcatc tgcggcaaac ctacacctac gatcatactg ccgatcaatc
    13141 ggtcccctct caaatcgatt ccttctgaaa aaatcccgcc aagtacgcaa aatccaatca
    13201 atgtttcagc gggatttgtt tgaaattttg ttaaaaatcg ttcgcgttct tcctcattca
    13261 tgtccgtttc ttggattagc acattgctgt cgggatattg ctgtctgaat gcttccaccg
    13321 caagatctaa atattgataa gaaggaaaga aaacgaaata attcccgacc tttccatcag
    13381 caaaagcatg aatcgtctct actaattttg ccagactgtg ttcccttttt cgataagtcg
    13441 tctctaggta atttgcgatg aaaacttgct ggtttccttc tgggaaagga ctcggcagtt
    13501 tgtaagccag acttttttgt ccccccagtg tttcctgata ataagagagt ggtgaaaaac
    13561 tagcagaaaa aagaatactg ctttttcctt tatccaacga ctgctcaaga aacaagcttg
    13621 gatcaataca aaactctttt actatcagat cgcgatacgt tttttcgact gttgtttcgt
    13681 agtgatcatc ataaaattca ctgaccttca aaaaatgaag caaatcaaaa taataaggaa
    13741 gcaattgttc ttgtgatggg tgctcaggaa attctgccag ccattcttta attttttcgc
    13801 ttaattgata acctgccttt actaaagatt cagccggtgc tttttgatga tgataatccc
    13861 aatgatcttc ttttgctatt tctcggatcg aatcaaattc ttttaatagt ttgttgaaac
    13921 ggcgatgaag ttttttgaat tcctttggta tagcttcctt acttcgtttc gttttttcat
    13981 aagatagttc tgccgaatac atctctcggg aacgattcac caaattatgt gcttcgtcaa
    14041 tcaaaaacag atagtcctcg tttttttcat cttcaaaaaa acgtcttaaa taaacagttg
    14101 gatcaaataa ataattgtaa tccccaatga ttacgtcaca ccagatactg acatctaatg
    14161 atagttcgaa aggacaaagt gtatgctttc ttgcataagt ttcgatgact tcacgagtga
    14221 tctgattctc atgattcaaa agatcccata aaccttcatt aatccgattg taataaccat
    14281 tggcatatgg gcactgatct ggattacagg tcgtctcatc taaaaagcag atcttgtctt
    14341 tcgccgtcaa agtgacactt tttacttcag ctcctgtatc ttttaatgct gtcatagcat
    14401 cttcagctat ttgtctagtt attgttttag ccgttagata aaagatcttt tctccctctt
    14461 cttcccctac agctttcaaa gcggggaata acgtagagat tgtttttcct gttccggtag
    14521 gtgcttctac aaacaatttc tgtttcgtac ggatcgtttt ataagcagct accgctagtt
    14581 cacgctgacc tttcctatac tcatcatggg ggaatttcaa agccatcaaa gaggcattgc
    14641 gtacagtgcg ccagttttct tgaaaaacaa gccattcttt ataatcttca gtcagctttt
    14701 taaaaaaagt ttctaactgc tctcttgtct gatgttcaac ctttctcgtg atcacttctt
    14761 ctgccgtctg gaagtaagtc aattgcagat ttatttcttg taaatcatgt tcttgactat
    14821 agatataggc atacaccctt gcttgatgaa agaaaagttc cacttgttct ggttctaaat
    14881 cttcaaatcg tggttcggag gttttgattt catctatata ataaataccg tctcttttga
    14941 aaataccgtc tgcacggcct tcaacgatca ggtaatattc atctaattga actgttgttt
    15001 ttaaaaagac ttctttttca tagttttctc ccgcatcttt ttggagcttg cggtggattc
    15061 tcgctccttc taatgcagta tggttacttt ttttacgatt gtcgatactt ccacgtcgca
    15121 aaataaattc tactaagtgc cgcacagata tttttgactg tttgatcatt tccaccacct
    15181 ctttctctct aagattacct gaaaaaacct agtttagcaa aaagagctct ctttccaaac
    15241 ataaaaaaac accctgctga ttaggcaggg atataggata tagaatttgg atttttggat
    15301 gaatctaatg taacaaggac atattaaaat gctatgaaaa cttactttcc ttctctttag
    15361 aatatccaaa tacttttcat atagtctgat aattcagata agtaaagaga aataagtacc
    15421 tgtttttgtt tcctagcaat gaatgcgtta tactaatgga tgaaagaagt tttggctatc
    15481 aaggaggaat gcacatgcat atcgaaagat taaaaacagg aatgattgaa gaaaactgtt
    15541 atttggtcta caacgaagaa gcacttttga tcattgaccc tggagaagat gcggaacgaa
    15601 tcaaacaaga aatcaaaaaa acaaaccaac agccggtagc gattttgctt acccatactc
    15661 actacgatca tatcggtgca gtggaacctt tacgcgcgta ttatcaaatc ccagtttatg
    15721 tcagcccact agagcaaaaa tggctaggtg atccgatttt gaatctttct ggactcggta
    15781 gacatgatga tattgctgat gttattgtct cgcctgcaga atatgaattt gaaatgaaaa
    15841 cttatacttt aggaaacatg tcttttcgcg tcgtccctac cccagggcat tctataggaa
    15901 gcgtcagctt tatttttgat gattttgtcg tttctggcga cgccctgttt agaggaagta
    15961 tcggtagaac cgatctgtat acagggaatt tagaacaatt acttcatagt attcggaccc
    16021 agctgtttgt attacctgat gaatttgcag tctatccagg acatggcgat gcaacgacga
    16081 ttgaacagga aaaaaggaca aatccttttt tcaatcgtta aaacgactta ggaggagaat
    16141 aaaatgacag aaactacact ttatatcgta cgacacggaa aaacgatgtt caatacaatt
    16201 gaacgagtac aaggctggtg tgatacaccc ctcacaaagc aaggacaaga aggcattcat
    16261 tatttaggtc gcgggttaaa aaatgtcgac ttttcatttg cttattctag tgacagtggt
    16321 cgtgcaatcg aaactgcccg tattattcta ggagaacatt ctaagggaaa agaaattcct
    16381 tattttattg aaaaaaggat tagagaatgg tgtttcgggt ctcttgaagg tggatatgac
    16441 atggaaatgt ggggtgtgat ccctcgtgta ttgaatttta aaacatatga tgaaatgttt
    16501 accacagaag tttcttttga acaaattgct aatgctgtat acaacttaga taccgctggg
    16561 tgggcggaaa cttatgaaac attgcgagac cgtgtatgga caggatttga agatatcgcc
    16621 catcactgcg aaaaaaatgg tggtggaaat gttttagtcg tatctcatgg tttgacgatc
    16681 gcctttttgc ttaatctgat cgatcctaaa cagcctgttc gtgctgggct tttaaatggc
    16741 agcgtaacaa aggtcactta tgcaaacggt cgattttcta tccacggcat caatgatacg
    16801 tcttatatcg ataacgggaa aacacatagt cgcgactcgt ttctataatg gggatatttt
    16861 aatttttacc agagaatcat aaaaggcaaa gtctgaacga gggctttgtc ttcttttttc
    16921 tcttcgtgta cttatctgct ttatttctca agtttttctt aatattaaaa aacagattgg
    16981 gtttctcaaa aaataacggt atactataag atgtatctta attgggaagg aaggaattta
    17041 tgttttttgc aaatattatc aaagcagtga ttctcggtat tattgaagga ataacggaat
    17101 ggctgcccat cagtagtact ggtcatttga tcctagcaga tgaattcatc aaactggatg
    17161 ccagttcaca atttatgtcg atgttcaatg tcgttatcca attaggggcg atcttggctg
    17221 tcgtcgttct ttattttcac aagttaaacc cattttctcc aagtaaatca acggtagaaa
    17281 aaaaagatac gtggacactt tggtttaaag tgatcgttgc ttgcatacca gccatcatca
    17341 ttggtttgcc tttggatgat tggttagaag caaatttcca caagttctta ccagttgcca
    17401 ttgttttgat cgtttacggt attgccttta ttattgtcga aaaacgcaac aaaaacaaaa
    17461 cacctcgttg gacttctctg aatgacttta cgtaccaagc agctcttctt gttgggttat
    17521 tccaagtctt ggcacttgtt cccggaacat cccgttctgg cgcaacgatc ctaggatcta
    17581 tcttgattgg tgcttcgcgt tttgtcgcta cagagttttc atttttctta gggattcctg
    17641 tcatgtttgg cgcaagcggc ttgaagatcg tcaaatattt agctgaaggg aattcattca
    17701 aaatggaaga aactgttgtt cttcttgtag gaacattcgt atcttttgtg gtttccgtct
    17761 tagctatcaa attcttacta agctatttga agaaaaacga ctttaccgct tttggctggt
    17821 atcgtattat ccttggtatc attttgattg gttattggtt tattgcaatg ta
//
LOCUS       DACCQP010000052        17203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCQP010000052 DACCQP010000000
VERSION     DACCQP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.054"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(90..1109)
                     /locus_tag="IVO87_001917"
     CDS             complement(90..1109)
                     /locus_tag="IVO87_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7450088.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1131..2138)
                     /locus_tag="IVO87_001918"
     CDS             complement(1131..2138)
                     /locus_tag="IVO87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7450089.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2131..2796)
                     /gene="pgmB"
                     /locus_tag="IVO87_001919"
     CDS             complement(2131..2796)
                     /gene="pgmB"
                     /locus_tag="IVO87_001919"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7450090.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2789..5083)
                     /locus_tag="IVO87_001920"
     CDS             complement(2789..5083)
                     /locus_tag="IVO87_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7450091.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5343..7508
                     /locus_tag="IVO87_001921"
     CDS             5343..7508
                     /locus_tag="IVO87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7450092.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7812..8645
                     /locus_tag="IVO87_001922"
     CDS             7812..8645
                     /locus_tag="IVO87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7450093.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8747..10447)
                     /locus_tag="IVO87_001923"
     CDS             complement(8747..10447)
                     /locus_tag="IVO87_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450094.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10954..12114
                     /locus_tag="IVO87_001924"
     CDS             10954..12114
                     /locus_tag="IVO87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7450095.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12130..12573
                     /locus_tag="IVO87_001925"
     CDS             12130..12573
                     /locus_tag="IVO87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450096.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13056..13586)
                     /locus_tag="IVO87_001926"
     CDS             complement(13056..13586)
                     /locus_tag="IVO87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7450097.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13691..14242
                     /locus_tag="IVO87_001927"
     CDS             13691..14242
                     /locus_tag="IVO87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7450098.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14395..14868)
                     /locus_tag="IVO87_001928"
     CDS             complement(14395..14868)
                     /locus_tag="IVO87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7450099.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15006..16478
                     /locus_tag="IVO87_001929"
     CDS             15006..16478
                     /locus_tag="IVO87_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7450100.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16475..17185
                     /locus_tag="IVO87_001930"
     CDS             16475..17185
                     /locus_tag="IVO87_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7450101.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5351 a         3092 c         3533 g         5227 t
ORIGIN      
        1 agataaacaa tgtgttagaa gtagtatctt cttttacata aaaagctgac cataggataa
       61 atggtcagcg tatttagtga gtcagctatt tagtatgggt tttctaaagg aacgactgtc
      121 tctcttttga tcaagcgatg agggatgacc aaacgtacgc cactgctcaa ttcttcatgg
      181 atcaattcgc gcagcttttg catagcgaca cgtcctaatt cggagatatc gatatcgata
      241 cttgtaaggt aagggtgtgt taatgttgag aagatcgagt tgttgaagct aatgacggag
      301 agtgtctctg gaatgacata gccatacatt tgcgccaact gcatcgtgcg aagggcgaag
      361 atatcgtcaa tgaccaccaa tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac
      421 agataatcct ccggatgctc tagtactaca ctggataacg caggaagacc agccatcatc
      481 attgcttttt gataaccgaa atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc
      541 acaaacagga tattcttatg tccattctgg atcaaaaatt ctgttgcttg tttacctaga
      601 agctggttat cattatctac ataaacgatt tcgttctcat gatgataagg ctgtccgatc
      661 aaagtgaaag ggatgtggtt ttggtataaa taatcaatga cagtatcttg actatctgaa
      721 tacactagaa taaaaccatc tacttgtttt tgtaaatgca tacgctggac gttctctaac
      781 agcaattcaa aatttttagc agtagcgatt gctgtagtca tatcataatt ccgcgcttct
      841 tcgttgattg cttccatgat ctcaagataa aatgggttac cgatccgctc ttttgaatca
      901 agagggggta agattacgcc gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc
      961 atgttaggaa cataacctag ttcatccata atttttcgga ctttcttctt tgttgtctcg
     1021 gaaatactag gatgatcgtt gatgacacga gagacggtcg aggtggcaac accggctttt
     1081 ttcgccacat cttttacggt aatagccatg ataaaaacac ctttctcttg ttattgtgca
     1141 gagaaccgat aaatcgtctg actgatccgt tcttgaccag gatggaggtt gatcgatcca
     1201 aatttgggga aatgaacgat atcagggatt tcttgcggtt cgattgctaa tccgtagttt
     1261 gaatgcatct gttttccatt gacagagaaa tcagcttcga atccagtcgt agaaaataat
     1321 accatacttt tattagaagt tgctatagtc atttggcgac cagaatgctt ctcgaataat
     1381 tgtaaggaag ttttggaaag cctaggatga tgcaaaagaa atacatcatc tagtccatct
     1441 ggataacatg caaggatctc ttgcagtgtt ggggattttc taaagtcgat tggcagtttt
     1501 tctttttttg tccaatttcc agtaggcagt ttttcttgat ctaattcaag cacaccatct
     1561 aaaaagaccg ttaactgatg ttcatagatg tctcttgttc catttcctga aaggttgaag
     1621 taggcatggt tggtcggatt gcagatggtt tcaacaaggc tactagcaga ggttgtcatc
     1681 tctaattcgt tttttgttaa acggtaagtg atcgtagcag tgattggtcc tggataggta
     1741 gaaaattcat cttttaagta aaagaccaca cctattgatg atggagtttt gaaaacttct
     1801 acttcccaaa attggaaaga ccagcctttc gtgccaccgt ggatatggtg tgatccgcag
     1861 tttttctcca atctatgctc accccaagaa gcatcccgga tccttccggc tacaggtccg
     1921 acagttgctc caaaaaagct tttgtctgtt agaacatctt cgaaacgatc gtaagacaat
     1981 aaaacatttt ctgattttcc ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg
     2041 taattgagaa gaacgacttc taagtagtcg ttcttcaatg tgattttctt tatttcagtc
     2101 tctggaactg gtacgacgga gatctctgga ttatccatgg tcatgccact tttctttcaa
     2161 gtaatcgatc gtaagaaaag tcgtatcagg gacaagcggg atctcctctc caaggtcttc
     2221 agcacgtccc acgccaattg gttgagcgcc acttgagagg atggcttgga taccggcttg
     2281 cgcgtcttcg atcccgatac attcttctgc ttttagtccg acagcttttg ctgcaaggag
     2341 gaagatatct ggcgcaggtt ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa
     2401 gtaaggtgta agacccattt tttcaagtag gaatggcccg tttttacttg cagaagcaag
     2461 agcaatcttg attttttctt cttttaaaga atccagtaaa ggcacgaccc cggggaagac
     2521 atcttttggt tcgatcgtct ggatcatctc taaataataa tcgttttttc ttttcgctaa
     2581 ttctgcaaat tcttcctttg aaaaagagtg ttcttttccg ccataagcta aaagcaaaga
     2641 gagtgagtct tctcggctga cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat
     2701 gccgatttcc tgaccaagct ttcgccaagc acgataatgg aattcagcag tatcggtaat
     2761 gacaccgtct aaatcaaaca ataccccttt aaacatttgc ttcctccttt cttagcgtta
     2821 tttctagacg atccgctaaa gaataagact gttcatagac gtaaatttct agaggagaac
     2881 cttctaataa ctcgaaagta acttgttcat ctgtgactga gatgaacaat aaacgtccgc
     2941 gataattgat atggaaagaa taagctttcc aagtttttgg cagaaatggt gcgaatctca
     3001 actgatcatg ggcagttttc atttgagcaa atccttggac aatcgacaac cagctaccag
     3061 tcatggaagt gatgtgcaag ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc
     3121 gagctgttcg ctggtacatt tctacagctt tgctttccat tcctaattct gctgctaaaa
     3181 tcgaatggat acttggcgaa agggaagact catggacagt cattggttca tagaaatcaa
     3241 agtttttttg cttttcttct tttgtaaaag tatcattgaa gaaataaatc ccctgtagta
     3301 catctgcctg cttgatgaag caagatctaa ggattttgtc ccaagaccaa tgctgattca
     3361 gaggaagatc gcttgtggga atatcggata caggcatgag atctttatct aagaatgtgt
     3421 catgctggac aaagattcct aattcttcgt caactgggaa gtacatgttc tctgcgatat
     3481 ctttccattt ggctagttct tcttcgctga tagtgatagc agcttctttt tgataagtta
     3541 aataattttc tcgcgtgtaa cgcaagaccc aagctgcgat cgtatttgta taccaattgt
     3601 tattgatatt gttctcatat tcgtttgggc cggtcacacc gtggatcatg tatttatttg
     3661 ctcgttttga aaaatggaca cggtctgccc agaagcgtgc gatttctgct aaaacttcca
     3721 gtccttcatt tttcaaatat tccgtatcgc cggtgtaatt cgtataattg tagattgcat
     3781 aggcaatcgc gccatttcgg tgaatctcct cgaatgtgat ttcccactcg ttatggcatt
     3841 cgacaccagt gaaggtgacc ataggataaa gcgcgccttt taatccttgt tgctgagcgt
     3901 tgtggattgc ttgcggcagt tgattatgcc gatatttcag cagattctta gttacttcag
     3961 gtttggctaa agcaagataa agagggacag catacgcttc tgtatcccag taagtggctc
     4021 cgccgtattt ttctcctgtg aatcctttgg ggccgatatt caatcgttca tcttcgccgt
     4081 agtaagtaga gaatagttgg aacaaattga atcgaatacc ttgttgtgct tcttcgtctc
     4141 cttcgatgac cacatcggca agcttccatc gttctgccca agccgctgtt tgctcttctt
     4201 ttgcttggat ataattgttt gtctggttcg ttaattctga gacagctttt tctgcttgct
     4261 gcgcttctgg aatatctcta ctagtaagaa cgataatttc tttttccact gaccatgtct
     4321 caccttgaga taattctgca gacaagcaac catgtaatgc taaagtatct tcgtgatagt
     4381 cagggatgac tgattttcca ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt
     4441 caatcccaaa gttgtttgga atagttttag tcgtcaaata gccgatttct tctatagttc
     4501 cacgatcaat ctcattccaa aaatgttcat catagttgct gtcttcgttt tgaacatttc
     4561 catccaattt tgatacgagt ttgactgttc cgctaccttc aagcatttct atagttaaat
     4621 agatataagc tgattctttt ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt
     4681 tattagtaga agtagtgatt gtgaatgtac gagataatac accatttttc atatctaatt
     4741 cccagtaaaa gtcttctggt tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa
     4801 tatctatcgc gataaagttc aaggcgttga tcacttttcc aaagtattct ggataaccat
     4861 ttttccacca gccgacacgt gtcttatcgg gataccagac acccgccaaa taagtacctt
     4921 gatggtggtc ccctgagtaa gtttcttcaa agttcccgcg catccccata taaccgtttc
     4981 caatacttgt taaagattct tgcagtcgaa gatttttttt atctaattgt gtcgtgctga
     5041 ttttccatgg atcaatagca aatagtcgtt tgatctgttt catttttctc ctcctaatga
     5101 gctgttttat acgttcataa taaacgcaac cgattgcaca agtcaataga caaaagaaaa
     5161 tatttcatag aaaaaagctt aaaaaaataa atttttagga gaaaattata gaagggaaca
     5221 cacgtattat aggttttata gaatggtata gacagaaaaa taaaaaataa agcatttaca
     5281 aaaaagaaaa cgcacgctat aatacgttta acgaaaccga ttgcgtaaag gagctaaacg
     5341 gaatgaaaaa gatatttagt tttgaatttt ggcaaaaatt tggaaaggct ttaatggttg
     5401 tggtagcagt tatgccggca gctgggttga tgatcagtat agggaaatca attcccttga
     5461 tcaatcctga ttggacgccg cttgtcacaa cgggcggagt aattgaaaat attggttggg
     5521 caattattgg taatcttcat attttatttg ctttagcaat aggaggaagt tgggcaaaag
     5581 aaagagcagg cggagcattt gcagctggga tctcatttat tttaatcaat cggatcactg
     5641 gggctatctt tggtgtcaca agtgacatgc ttgcgaatga agatgcattt acccacactc
     5701 tgttcggtac gaagatcatg gttaaaggat tttttacaag tgtattggaa gcaccagctt
     5761 tgaatatggg agtattcgta gggatcatcg ctggatttgt cggggcaatg gcttataata
     5821 aatactataa ttatagaaaa ttgccggatg ctttgtcatt tttcaatgga aagcgctttg
     5881 ttccgtttgt tgttatctta tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc
     5941 caaacgtaca ggctggaatc aataattttg gtttatggat tgcagaatca caagaaagcg
     6001 caccgattct agcaccgttt ttatttggaa cattagaacg tttactattg ccatttggat
     6061 tgcatcatat gctgacgatt cctatcaact atacacagct tggcggatcg tatgagattt
     6121 tatcaggcgc acaggcgggg acacaagtat ttgggcaaga tccgttatgg ttggcttggg
     6181 ccactgattt agtgaatctt aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc
     6241 ataactggac gccggctcgt ttcaaagtag gacaaatgat tggttcttca ggtatcttga
     6301 tgggaatggc ttttgcgatg tatcgtaatg tcgatcctga taagaaagca agatataaat
     6361 caatgtattt ttcagcagcg ttggctgtct ttctaacagg tgtaacagaa ccattggagt
     6421 tcatgtttat gtttgctgct gtgccattat atgtcattta ttcagtgatc caaggggctg
     6481 catttgcgat ggcagatatc ttgccattgc gggtccattc atttgggaat atcgaattgc
     6541 tgactcggac accgttagct atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt
     6601 tgtgtgtgat cgcttttggt gttgttacgt atttcttagc aaatttcttg atcaaaaaat
     6661 tcaactttgc tacaccggga cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg
     6721 tatctaacag cggaacagga acagcagatc agcaagtggt ccagatcatt catttgctgg
     6781 gaggaaaaga aaatatcaaa gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa
     6841 atgatcgaga aaaagtaggg actgaagaag cctggaaacg agcaggggcg atgggattga
     6901 tcgtcaaaga caatggagtc caagcagttt atggaccaaa agcagatgtc ttgaaatcag
     6961 atatcgaaga tctgctgcaa tcaggagcag agattccaga gccagaaatg gtttctacag
     7021 gaaatggaca agcaggtagt aagcaatact taggtataga acaagaacta gtttccgcag
     7081 cttctggaga agtgattcct ttatctgaag taaaagatcc ggtattctca caaaaaatga
     7141 tgggtgacgg ctttgctgtt attccaacat caagagaagt tgttgcgccg atcgctggaa
     7201 aagtgacaag tattttccct agcaaacatg caattgggct ggaaacgaaa gatggtatcg
     7261 aagttttgat ccatatggga atcgatacag tccaaatgaa tcagccagct tttgagattg
     7321 ctgtcaaaga aggccaagaa gtaggaacag gaacgaaatt agctgagatg gatcttgatg
     7381 tgatccaaaa cgaagaaaaa gatccaacga tcatgatcgt atttacaaac gataaagtag
     7441 aagaagtagt gatcaagcaa ttaggtacag tgacagctgg taaagtgatc ggctccatta
     7501 aaatgtaagg agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca
     7561 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgagaagca gcagtttgaa
     7621 tactgtttag tggagaggtc gtgaaaaagc tgccctgcac caagtgataa aaacagccaa
     7681 acaaaaaata gctcctggtc cattgagctt attactgagg agttagcagg agaacactat
     7741 ttgctggaga ggttgtgaca aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa
     7801 ggagaaaacc aatgaaattc atgacactca acacgcatag ctggcttgag ccagaaccag
     7861 agaaaaaatt gcaagaatta gcagataaga tcctgttaga agattatgaa gtgattgctt
     7921 tacaagaaat caaccaatta ctggaatctg aagaagtaga acctgcgaag ctaatgaagt
     7981 tctgtccggt aaagaatcaa gtacctatcc gtaaagataa ttttgcctac cgtttagttc
     8041 agcttttaaa ggaacacgga aaagagtatt tttggagttg ggaaatgagc cacatcggtt
     8101 atgataaata tgaagaagga aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc
     8161 ttaccgtctc ccaatttcaa aagaaagaag attatcagac acgcaagatc ttgattggga
     8221 aaacaaagat agatgatcag gacatttttg tctcgagctg ccacttttct tggtggacag
     8281 ataaaaaaag tggcttttat tttgagtgga aaaacttgga aaactacttt ttggaaactc
     8341 gcgtaccact ttttttccta ggtgatttca ataatccagt agactctcaa ggttattata
     8401 ctgtaagaga aagttgcctt cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa
     8461 aagcagctac tgtggaaaag aaaatagatg gttgggaaca aaatacagaa aaattgcgta
     8521 tagattttat tttcgtccct gaaggaatgc aggttaaaaa atatcaacgg atttttgatg
     8581 ggatcgatag tccaatcatc agtgatcact acggggtgga aatcgaatta gatgtgaacg
     8641 aataaaaaat ataacatata acataaaaaa actgattaaa ataaaactct ccaaacgatt
     8701 ataccgtttg gagagttttt tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa
     8761 gcttgtatct atgataaata aataaacata gcaaggtaaa ccagccaatc aaagtaagag
     8821 aaagaatgaa atagaaatag attggcgttt gataatgaag cgtgagctcg ttgattcctt
     8881 tttgctgctt cacgattggg gtcccgatat ctgttacttc ataatcagtc aatgtttttc
     8941 cattttgctg tagcacagtc cgatcgtaga cgataacagg gatatgaaca gttttgtccc
     9001 cagtgttttt ccacttgatt gtcaaaagac cgcctggatg tgtttttata aaagttgttt
     9061 cgttttctag tatttcttcg ttataccggt cgtatttatt gtcttttgtc tccttataga
     9121 ttggcagata gtcaggtgtg gttttttgga aaacgaacaa gctggtagac atatctttgc
     9181 tgaagaatgt tttccttgct tcttccggcg tatcgaatag ataagtatgt cgactgacaa
     9241 atgtttcatt ttcccacttt tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa
     9301 aaataagaat gccaaatata cttaaaagac ggaaccactt ttgttcagac cactggttca
     9361 gtgaaagggc aaggtaaagt agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa
     9421 actggatcaa actaataaaa ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc
     9481 tagtagaaag aaaaaagaaa acaagacttg tgtacaacac gattttttgg aataaggtgt
     9541 tcttttgttg aaagaaaata aaaagcaaaa aacctgataa tatcaatata ggaaaggcct
     9601 tagggtaatg gagccaataa ctgccatcca gatctatcgc cttttgactc atttctctgt
     9661 taacaaaggg aggaagaatc ttattcccac tgtaaacaat agctaatcct gcccaaatat
     9721 ttgctgtcag aacaaaataa agcaagaccg acttaatcag ttgaagcagt gcttttcctt
     9781 tttcagtctg gttaaaaaag taataaagat aaaaagggat atacatcaaa gctaacatga
     9841 tcgaagacag taaatggatc tgtgtcataa gtcccactga gacagcgact ttgataacag
     9901 ggaacttttt atgaaggacg aaatcagcaa tagggatcat acataaagga taaaaacaag
     9961 tcccccagct agtgaatcct tggttgatag accagtattg aatagaatac gtactcatga
    10021 ataaaacggc tactggtaca cttacccaat ttcgaacatt gacatatctc agtaaaatca
    10081 gcatagaaaa tcctgaaata agataaagaa tgaaatttga aagaatctga taatgaaacc
    10141 agctacctgc aagcaacacg atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg
    10201 cattcacgat cctgccactt tgctggaatc cataaaggga aggaaaataa tggaaatccc
    10261 catatttaat ttgttgcgcg gcatcataga accgattgta atggaatggg gcatcagcac
    10321 ccaaaattag tttatgtgtg aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa
    10381 ttgctaaaaa tgggccatat tttttcaatt gtcgaactcc tttgcaaact ttagataaat
    10441 tcatcatata tcctatgcga gaggtaggca agaaattaga aaattcatga aaatatttat
    10501 gaattttaca agaaaaacat aagtgaaagt tctaaggaga gaggatgcga cataagtttt
    10561 gttgcatctc cttgttttga aacagaattt ttcttaatac tcgaaacaaa gtagcttttt
    10621 catgacctct cggtaaacgg tgttcaatca gctgcttctc ggtaataagc tcactagcct
    10681 agaaaatatg agaagctatt ttctgactag cgctcttatt actcaaagca taacgcagtt
    10741 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    10801 caacaaaaaa tatgagtagc tatttttttg ggctgtactt attacttgat gcaggacagc
    10861 tttttcacaa cctcatcggc tttcattttc tatttttttc ctgaacccat ataataaaag
    10921 aaagaacaaa aaaacttgca agggaggaca catgtgaagc agagtaagca aaaacagtct
    10981 cgtcgacttt tgtttcgttt gtttttatta atatgtatcg tcgtagggtt actgatcgct
    11041 ttatatccat tttatgtgga ttctttaaat agttttattg atcaaaaacg tatcgaacaa
    11101 gtacaaaaac ggaccgcagc tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaac
    11161 caacgattaa ctgaccaagg attcaatcct ggtactgatc ctttcaatga aaagaacaga
    11221 aatgaaagta caacatccag tcaattagag gaatggctga tcggaagtat caatatacct
    11281 aagatccaat taaatatttc actttatgat cggctcaatg gtaggatcct tgaaaatgga
    11341 gcaggcgtct tgcaaggaac ctctttcccg cttggtggaa attcgacgca ttcagtgatc
    11401 tctgctcaca gcggtttacc taatcgacgg ttatttaccg aattagatcg tttagaaaac
    11461 ggagacacgt ttattttgac tgttttgggg gagaaattgg cttatcaagt taaaaatata
    11521 caagtggtac tgccagatga cacgtctgtt ctaacgattt cagaaggaga agacttagtt
    11581 accttgctta catgtacgcc gtatatgatc aacacgcatc gtttgctagt gacaggacat
    11641 cggattcctt ataacgaatc ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc
    11701 atgcggcaat ggtttattct tgcaggaacg ataatagctg tagtcatact gttagtgttt
    11761 atcggacgac taatctatca gtatagatta agtaaaaaag tgcttgattt ttcatttatt
    11821 attagtgatt cgtcagggaa accggtaaat ggtagttcat tcattttgaa acataagaaa
    11881 aaaacactta ctagaaatgg tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc
    11941 aagctggatc aattgcctgg tggaacttat cgaatcgttt cagctgatga tcctaaagtt
    12001 gctgtttctt ttggtattag aaaagtaaaa caagagaaga tatacttttt tgaggggcga
    12061 aaacttgtaa aagagcttca aaaaaatggt ttttatttta aaatcaatga ataagtaagg
    12121 agagaaaaaa tgatccgcac aacaacaaaa ttgtctgcca aagaactgga atctgttcta
    12181 tcgatttggc tgcatgctaa cttggattct catgaattca ttccatcaac ttattgggag
    12241 aatcaagtag aatctgttag gaaagcttta ccagatgcag tgatttactt agcagaaata
    12301 gatcaagaaa ttattggttt tgctggggtg attgatcagt ttatcgcagg aatctttgtg
    12361 aaaagggaat accggcaaca aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc
    12421 aataaaacac tgactttgac agtctatcaa aaaaatcaat cagctattca tttctatctt
    12481 tcacaaggtt ttacaattca aacacagcaa aaagatgaag cagtaaatga ggaggaactt
    12541 gtgatgaaat ggaatcaaaa cgctgatttt tgatgaacag aataacgaaa taatgctccc
    12601 agcctaattt tttgctggga gcattatttc attttgaagt cactaattta actgtgtgaa
    12661 tatgcagttg ttacaatctt tgataaacgg tgttcaatca gctgaccctc ggtattagct
    12721 caaaaaacgg caaaatatga ggaactattt ttccatttgt ttctctaata ctcaggtcaa
    12781 aacgctgatt tcacaacctc ttgataaacg gtgttcaaac ggctgcttct cggtaataag
    12841 cccactagcc tagaaaatat gaaaagctat tttctaacta gcgttcttat tactcaaagc
    12901 ataacgctga tttcacaacc tcttgataaa cggtgttcaa tcagctgacc ctcggtatta
    12961 gctcaaaaaa cggcaaaaca tgaggaacta tttttccgtt tctttctcta atactcaggt
    13021 cagaacgctg atttcacaac ctctctttca caaccttatt ttactcgttt ataattttca
    13081 tcgaagtatt ttccgctacg gatatcatct ggcattccat cgtatggttc ttcgctaata
    13141 acatcttcat tgtctatacc tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca
    13201 agtaattttg gataagtttc atatttttca cgagctgctt ccatttctgc gatatgttcg
    13261 ttttggaaag taacagtgat ttctggatgt tcttcaaccc atttagggaa aaagatcatt
    13321 ccgtgaagtt tcttttcatt tggcataaag tccaatgcca cgtctgtccc tgttggctct
    13381 gtccaagtga ttttgaatac gccgtccgtc aatttaacga tatcagcttc ttggtctttt
    13441 acccaacggc ctttgaccat tccgccatgg atacggtaat caacagtatg gtcatttttg
    13501 gcataccatt catattccca accattgtca tacgtataga taaagtgagt tccaataaaa
    13561 tcttccaatt ctttaaattc tttcatgttt aagcatctcc ttataaatta aaacatgtaa
    13621 taaactacat gttttcgatt actcatttat gatagaatgg atttaaagat ttgtcaagga
    13681 gggaaaactg atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa
    13741 gaagaagatg acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg
    13801 gcaagcaaaa catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat
    13861 tcagcatcaa gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga
    13921 agaagggaag gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa
    13981 tcgagacgaa tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt
    14041 agaaattatt cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg
    14101 aaaaaaaacg atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat
    14161 tttagcgcat gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga
    14221 agaaagtaac cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata
    14281 tgaaagaaac gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt
    14341 atttatgtag catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag
    14401 ttcttttaac aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa
    14461 ttcgtctgtc tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc
    14521 gatttgtaaa atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa
    14581 aactttacgg acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga
    14641 caaagtaaat ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa
    14701 acgatatttt ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc
    14761 ccaatacctt aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac
    14821 aaccaattga tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc
    14881 ttttgtcctc ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac
    14941 aaatagcgaa tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga
    15001 gaaatatgag taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt
    15061 tagttgttgt cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga
    15121 cagcttttcc tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa
    15181 cgactggttt cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata
    15241 aattcagttc aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta
    15301 cttgctttac agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg
    15361 gtgtgggagt atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa
    15421 aaagaggatc agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc
    15481 ctacattatc tggttatatc attgataact accattggcg tgacttgttt ggtatggtac
    15541 tgccaatcgt tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat
    15601 tgtcaaatcc tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt
    15661 tactttacgg attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt
    15721 tcttagttgt tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac
    15781 atccattctt ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag
    15841 ctggtgtaac aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa
    15901 atattcgtgg agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca
    15961 tgggggctat gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag
    16021 ccatcacagg gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa
    16081 cgactccagt tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg
    16141 tcatgatgcc ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg
    16201 ggacagcggt aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga
    16261 tcagtgtgtt gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata
    16321 cgccattagc ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt
    16381 ttatcgtagc gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa
    16441 aagaacctgt tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag
    16501 cttgcttgct tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc
    16561 tttgattttc ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa
    16621 tcactttgga atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga
    16681 ttcccaagga gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta
    16741 tctttaccga acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac
    16801 aaataaagta gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga
    16861 atacaaaaac gacatgtata aattctggtt caatattgct gataacaatc acgaatatga
    16921 cagccgagtg aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc
    16981 agccaagtta gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc
    17041 ttatgttcaa gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga
    17101 gcaagaacaa cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt
    17161 agtgaaagaa gcaaaaggcg aataatattt tgaacaccat gta
//
LOCUS       DACCQP010000053        16904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCQP010000053 DACCQP010000000
VERSION     DACCQP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.055"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..190)
                     /locus_tag="IVO87_001931"
     CDS             complement(<1..190)
                     /locus_tag="IVO87_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450102.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="IVO87_001932"
     CDS             complement(200..1015)
                     /locus_tag="IVO87_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7450103.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="IVO87_001933"
     CDS             complement(1277..1720)
                     /locus_tag="IVO87_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7450104.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="IVO87_001934"
     CDS             complement(1854..2192)
                     /locus_tag="IVO87_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7450105.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="IVO87_001935"
     CDS             complement(2180..2557)
                     /locus_tag="IVO87_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7450106.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLISPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="IVO87_001936"
     CDS             2781..3974
                     /locus_tag="IVO87_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7450107.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="IVO87_001937"
     CDS             complement(4140..4562)
                     /locus_tag="IVO87_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7450108.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="IVO87_001938"
     CDS             4957..5151
                     /locus_tag="IVO87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450109.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5642
                     /locus_tag="IVO87_001939"
                     /pseudo
     CDS             5148..5642
                     /locus_tag="IVO87_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5804..6976)
                     /locus_tag="IVO87_001940"
     CDS             complement(5804..6976)
                     /locus_tag="IVO87_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7450110.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="IVO87_001941"
     CDS             complement(7182..7997)
                     /locus_tag="IVO87_001941"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7450111.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="IVO87_001942"
     CDS             complement(8147..8593)
                     /locus_tag="IVO87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7450112.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="IVO87_001943"
     CDS             complement(8590..9594)
                     /locus_tag="IVO87_001943"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7450113.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="IVO87_001944"
     CDS             complement(9634..9963)
                     /locus_tag="IVO87_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7450114.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="IVO87_001945"
     CDS             complement(10089..12416)
                     /locus_tag="IVO87_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7450115.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13259..13552)
                     /locus_tag="IVO87_001946"
     CDS             complement(13259..13552)
                     /locus_tag="IVO87_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7450116.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13955..15133)
                     /locus_tag="IVO87_001947"
     CDS             complement(13955..15133)
                     /locus_tag="IVO87_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7450117.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15237..15551)
                     /locus_tag="IVO87_001948"
     CDS             complement(15237..15551)
                     /locus_tag="IVO87_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450118.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15658..15873
                     /locus_tag="IVO87_001949"
     CDS             15658..15873
                     /locus_tag="IVO87_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450119.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16173..16415)
                     /locus_tag="IVO87_001950"
     CDS             complement(16173..16415)
                     /locus_tag="IVO87_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450120.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16402..16830)
                     /locus_tag="IVO87_001951"
     CDS             complement(16402..16830)
                     /locus_tag="IVO87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7450121.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4928 a         3271 c         2934 g         5771 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgaa atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca taacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt
    12481 attttattga tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt
    12541 agtcttgtcc tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat
    12601 tattgaaaaa aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg
    12661 agggaagaat aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt
    12721 gttatctttg aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa
    12781 taaacggtgt tcaattagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc
    12841 tattccccat tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg
    12901 gaataaaagc atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct
    12961 gtccaaaatc taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat
    13021 tatctttatt catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt
    13081 actgacgatt gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat
    13141 tttgaataac gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg
    13201 cggtgtagaa ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct
    13261 aattttcaat tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa
    13321 atgtgattag ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta
    13381 tcgaattaac ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt
    13441 tcaataggat gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca
    13501 tgaatgtgtc taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc
    13561 tccttttgat tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc
    13621 ggttactctt aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag
    13681 atttattcta atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg
    13741 ttcaaattac atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga
    13801 taacaatgat taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat
    13861 aaaaggattt ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca
    13921 agaatcctgt aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa
    13981 tcagaggttt taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt
    14041 cagtatggcc taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg
    14101 ccattgtatg acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc
    14161 accgtttgac gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat
    14221 caagatacac aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac
    14281 ccaaagcctt aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc
    14341 tagcagaact aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg
    14401 ccgtttgctt atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag
    14461 caagcgcctc gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg
    14521 gtaattcctc atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa
    14581 acttgtcctc aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg
    14641 gcattctcac agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa
    14701 aaatcatatt tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg
    14761 ttccattaac gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca
    14821 aatcagacaa ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct
    14881 tatcataggt tatttccttt tgttcattta acgaacggta atattggaac cattgctcac
    14941 ctacaacact aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct
    15001 ccatttccag ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc
    15061 gatatgcttg aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat
    15121 ctgttttttc catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt
    15181 tggaaatctt tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat
    15241 aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat
    15301 tctaaaacat catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca
    15361 ccaagtttta ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca
    15421 gtatttgtta ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc
    15481 agtttttttt gtaaaattga atagaactct atttccagag aattaacatt tgttttaact
    15541 tccaactcca tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat
    15601 tgtaaaaaaa cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg
    15661 aaacaaggaa ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat
    15721 gcaatagcat tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag
    15781 agagctgaag aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa
    15841 aatatgccaa ccaaaatcga aatatggaaa tagtagtttt tctgatctat cagctgtttt
    15901 gccaaagcac ataggaatct cacctccccc cagtaatggc gggccgattt ggttacggaa
    15961 gtatcattat cgtagtcgcg attctcatat taaaatttgt cgcttaaaag ctaaccttgt
    16021 catggcgtaa ctagagaatc aaaatctaca tgtactgata gatttgctat tcatttgtca
    16081 aagagcgcta ggctattttc agcctatgga aatcctttat tttgacccga taaaaaactt
    16141 ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc
    16201 ttctcaatca attttgtttc cagttgtcta cgcatatatt catctacata cataaccatt
    16261 cctttttctt cttcataaac tggtttcatg gctaaccgtg aaatataatt ttgatatttt
    16321 gacagaacga tggacatggc aattgaatca ccagaagctg cggctttgat tgtcgttagt
    16381 ggtatgaatg gtatatatcg tttattttgc ttcatatttg acacccaaat actttcctag
    16441 ctcttccaaa gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc
    16501 gccaatttca cgatcattaa gaccgatgaa gaaggacata aaaatgattt cttgtctttg
    16561 actaggtaat gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc
    16621 aaggacaata aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc
    16681 taaccctttt tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt
    16741 cttttgaata tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg
    16801 ttgaatgtgg gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa
    16861 ctcgttttac ttctatccct ttccattatt actacaatct agga
//
LOCUS       DACCQP010000054        16587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCQP010000054 DACCQP010000000
VERSION     DACCQP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.056"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IVO87_001952"
     CDS             complement(79..786)
                     /locus_tag="IVO87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7450122.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IVO87_001953"
     CDS             complement(865..1203)
                     /locus_tag="IVO87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7450123.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IVO87_001954"
     CDS             complement(1314..2276)
                     /locus_tag="IVO87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7450124.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IVO87_001955"
     CDS             complement(2291..3622)
                     /locus_tag="IVO87_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7450125.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IVO87_001956"
     CDS             complement(3630..4310)
                     /locus_tag="IVO87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7450126.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IVO87_001957"
     CDS             complement(4529..5008)
                     /locus_tag="IVO87_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7450127.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IVO87_001958"
     CDS             complement(5135..6814)
                     /locus_tag="IVO87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7450128.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IVO87_001959"
     CDS             7362..8864
                     /locus_tag="IVO87_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450129.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IVO87_001960"
     CDS             8877..9539
                     /locus_tag="IVO87_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450130.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IVO87_001961"
     CDS             complement(9640..9843)
                     /locus_tag="IVO87_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7450131.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IVO87_001962"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IVO87_001962"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7450132.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IVO87_001963"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IVO87_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7450133.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IVO87_001964"
     CDS             complement(13807..14421)
                     /locus_tag="IVO87_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7450134.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IVO87_001965"
     CDS             complement(15027..15404)
                     /locus_tag="IVO87_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450135.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IVO87_001966"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IVO87_001966"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7450136.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4808 a         3573 c         2831 g         5375 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tgactgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcactt
//
LOCUS       DACCQP010000055        16504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCQP010000055 DACCQP010000000
VERSION     DACCQP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.057"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IVO87_001967"
     CDS             5..1087
                     /locus_tag="IVO87_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7450137.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IVO87_001968"
     CDS             complement(1305..2447)
                     /locus_tag="IVO87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7450138.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IVO87_001969"
     CDS             complement(2452..2637)
                     /locus_tag="IVO87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7450139.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IVO87_001970"
     CDS             complement(2991..3236)
                     /locus_tag="IVO87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450140.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IVO87_001971"
     CDS             complement(3230..3631)
                     /locus_tag="IVO87_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7450141.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IVO87_001972"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IVO87_001972"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP7450142.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="IVO87_001973"
     CDS             complement(5649..7415)
                     /locus_tag="IVO87_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP7450143.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="IVO87_001974"
     CDS             complement(7428..8222)
                     /locus_tag="IVO87_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7450144.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="IVO87_001975"
     CDS             complement(8232..9608)
                     /locus_tag="IVO87_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7450145.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IVO87_001976"
     CDS             complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IVO87_001976"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7450146.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IVO87_001977"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IVO87_001977"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7450147.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IVO87_001978"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IVO87_001978"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7450148.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="IVO87_001979"
     CDS             complement(13042..14673)
                     /locus_tag="IVO87_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7450149.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="IVO87_001980"
     CDS             14977..15648
                     /locus_tag="IVO87_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7450150.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         5354 a         3192 c         2212 g         5746 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcc
//
LOCUS       DACCQP010000056        16200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCQP010000056 DACCQP010000000
VERSION     DACCQP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.058"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..457
                     /gene="scm"
                     /locus_tag="IVO87_001981"
     CDS             <1..457
                     /gene="scm"
                     /locus_tag="IVO87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7450151.1"
                     /translation="SDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTD
                     TSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTN
                     KTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(612..1598)
                     /locus_tag="IVO87_001982"
     CDS             complement(612..1598)
                     /locus_tag="IVO87_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7450152.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1621..2187)
                     /locus_tag="IVO87_001983"
     CDS             complement(1621..2187)
                     /locus_tag="IVO87_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7450153.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2201..2617)
                     /locus_tag="IVO87_001984"
     CDS             complement(2201..2617)
                     /locus_tag="IVO87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7450154.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3074..5008)
                     /locus_tag="IVO87_001985"
     CDS             complement(3074..5008)
                     /locus_tag="IVO87_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7450155.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5310..6245
                     /locus_tag="IVO87_001986"
     CDS             5310..6245
                     /locus_tag="IVO87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7450156.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6335..7438
                     /locus_tag="IVO87_001987"
     CDS             6335..7438
                     /locus_tag="IVO87_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7450157.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7451..8404
                     /gene="menA"
                     /locus_tag="IVO87_001988"
     CDS             7451..8404
                     /gene="menA"
                     /locus_tag="IVO87_001988"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7450158.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9218..10564)
                     /gene="gor"
                     /locus_tag="IVO87_001989"
     CDS             complement(9218..10564)
                     /gene="gor"
                     /locus_tag="IVO87_001989"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7450159.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10698..11939)
                     /locus_tag="IVO87_001990"
     CDS             complement(10698..11939)
                     /locus_tag="IVO87_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7450160.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11957..12880)
                     /locus_tag="IVO87_001991"
     CDS             complement(11957..12880)
                     /locus_tag="IVO87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7450161.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13101..15593)
                     /locus_tag="IVO87_001992"
     CDS             complement(13101..15593)
                     /locus_tag="IVO87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7450162.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15600..16067)
                     /locus_tag="IVO87_001993"
     CDS             complement(15600..16067)
                     /locus_tag="IVO87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7450163.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4761 a         3387 c         2881 g         5171 t
ORIGIN      
        1 aagcgacagt acggatacat ctaccacaac aagtgatagc acggacacgt cagcttcaag
       61 tgacagtaca gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag
      121 tgatagcacg gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga
      181 tagctcagat tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac
      241 acgtcatgca agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt
      301 aactaataaa acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg
      361 aagtcaatcg aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag
      421 aatcactttt atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga
      481 gcaacgtcgc taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta
      541 caattcaatt tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa
      601 cctttcccta ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct
      661 tttgttcgaa gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa
      721 gaggccgcta acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg
      781 gcgtcgacat cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt
      841 ttgttttttt gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct
      901 agtacaggtt tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc
      961 tgaaaggcca tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt
     1021 gttccacttt caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct
     1081 gaaatatttt ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc
     1141 cctaattcgc cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag
     1201 gaagtggagt aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag
     1261 acagaaacga cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct
     1321 tcatcaacga tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc
     1381 aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag
     1441 gcaggacgca acagctttcc gccggcatgg atcgtttctt tgattgcttg ttcgacaggt
     1501 ttgtttttca ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt
     1561 aaagcaggat agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga
     1621 ttattttttg gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg
     1681 gaatcgttga agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat
     1741 ccctgataaa atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg
     1801 tgcaatgaaa gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact
     1861 aattccgatg atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata
     1921 actaagcaaa gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt
     1981 agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct cgttttttca tggtaaaaat
     2041 ggcaacgatc gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg
     2101 ataagggatc atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat
     2161 ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt
     2221 cgtcacctcc atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg
     2281 tttcgccatt tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt
     2341 cttttatccg tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt
     2401 tgtaagtata tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc
     2461 catccacacg cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa
     2521 tcatgactgg taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt
     2581 tgagcagact tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt
     2641 aactcaccca attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta
     2701 ataagaacga agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg
     2761 agaagcagca ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca
     2821 agaaataaga aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt
     2881 tttcgaagaa gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga
     2941 cacctgtgtc ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa
     3001 aaaggaggct gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac
     3061 gttctttatc tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac
     3121 ctagtgtacg ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac
     3181 cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg
     3241 ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc
     3301 cagcaatcaa tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca
     3361 ttgctacatc attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact
     3421 tctcaatcac agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt
     3481 gagtcgctgc ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag
     3541 cagcagaagc accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac
     3601 tgtttccagt gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat
     3661 tcccaatgat ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg
     3721 gcacactcca taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat
     3781 ctttgatatg gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat
     3841 caaagaagta tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat
     3901 ggatcttatc aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt
     3961 gatagtttcc tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg
     4021 cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga
     4081 attcttcata aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg
     4141 cctgcatata ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag
     4201 cagcgtcaga agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc
     4261 gtccgtcttt caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata
     4321 attccacgtt gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca
     4381 tattcaagat cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt
     4441 tataatcttt ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg
     4501 taaatccaga accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt
     4561 ccaatgcagc ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa
     4621 atgaccaaag ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc
     4681 cgcccattcc aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt
     4741 ttcgttcttt atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag
     4801 caaacaaatg ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt
     4861 catgcaactc ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat
     4921 cttctttttt cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc
     4981 ctacaacgac aatattttgc tttgccattg atcatccgct ccttatagac tatctatata
     5041 atgaatatat gataattctt ttagtttgat acttattaca caatctccat atattatata
     5101 gatgttctgt tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt
     5161 tttttcacga aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa
     5221 atagttgact tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg
     5281 gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg
     5341 ccgtattagc attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct
     5401 caaactcttc ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat
     5461 catctgcaaa agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa
     5521 aaaattattc aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca
     5581 ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat
     5641 acaacaaaaa catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga
     5701 atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc
     5761 actcatctga aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta
     5821 acacagatac aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg
     5881 aagaaaagaa ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca
     5941 aagtaactga atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata
     6001 cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta
     6061 ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag
     6121 aagttgttac aggtgctaca cacagctcac attcattcat catgtacgca caacaattag
     6181 tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa
     6241 aataatcaca taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag
     6301 tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt
     6361 aatcacactg ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac
     6421 aaccactgaa agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt
     6481 cttgttaggg acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa
     6541 acctgccttc gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc
     6601 tgagatcgat gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt
     6661 ctacacgtta gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat
     6721 ggcgattggt gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc
     6781 ccaagaagaa atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga
     6841 caaagaaaaa actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc
     6901 caaaggttac attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc
     6961 catcgttgac ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca
     7021 agactggaca gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat
     7081 taaagaacgg aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga
     7141 tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat
     7201 cgcaagtgtc acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt
     7261 cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac
     7321 ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac
     7381 cttcaaatta tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg
     7441 agtacgaacc atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc
     7501 tagtgtattt ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca
     7561 ttggctaaac acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc
     7621 tatcaataat tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa
     7681 tgtcattggc cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat
     7741 tgcatttgtt tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt
     7801 gatgggattt atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc
     7861 tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat
     7921 ggtcgtttat gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc
     7981 tggacaattt gctttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt
     8041 acccttggtg tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg
     8101 tgatattgaa aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt
     8161 cttattccag ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg
     8221 tatctatgaa tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt
     8281 ggatcaattc aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat
     8341 ggtcttattc aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat
     8401 ttaacagcat gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa
     8461 taaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     8521 gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt
     8581 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag aaactggctt
     8641 ttgaacaccg tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca
     8701 aatggaaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga
     8761 ttgaacacta tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct
     8821 tttttcactt gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac
     8881 tatatggtat tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca
     8941 cttttttaaa catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat
     9001 tttgaactta agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa
     9061 tcaaaatctc caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata
     9121 aataactaaa aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt
     9181 tgttcaataa aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt
     9241 ccgctcctgt tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct
     9301 tgatagccac ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc
     9361 cgacgattct ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat
     9421 catttaatgc aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc
     9481 catatttttc caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa
     9541 agacgactgt cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac
     9601 gttctgataa acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca
     9661 catcaccaac agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa
     9721 accctttttt gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta
     9781 cacgtccacc ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca
     9841 ccgtatattc gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta
     9901 ttccagcttc ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa
     9961 gtggtcgttc ttttcgatat gcccacatga cttcactacc taaaccacaa aatacacctg
    10021 aaagttcagc cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta
    10081 attcaaaaaa tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg
    10141 cacggcctcc agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga
    10201 tcgtttgact atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta
    10261 atcctctttg ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac
    10321 cggcgaaatc aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa
    10381 ttgtttccaa aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga
    10441 cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg
    10501 ctctgtttgc tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt
    10561 gcattttgtt cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc
    10621 tacagaaaac aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg
    10681 atatatcagc tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt
    10741 gcgactggca tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct
    10801 tcattatgca taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg
    10861 atttcatctc ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca
    10921 tcataagata atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat
    10981 aattgttcgt tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct
    11041 actttccata actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata
    11101 taactgtcta cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc
    11161 attcttcctt cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg
    11221 atcattgaca tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt
    11281 aaactgtttc gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat
    11341 tgagaacatc cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga
    11401 ccagaataag agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt
    11461 ttacgaatct ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac
    11521 ccttcttctt tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa
    11581 tggatcggca gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca
    11641 atcactgcag aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca
    11701 tttccttcat gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca
    11761 aaagcaactc cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca
    11821 tatgcattcc cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt
    11881 tccagtgttc ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt
    11941 tctttcatca tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta
    12001 attcacggtt ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt
    12061 gatcccagct accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta
    12121 cgaatttttc gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat
    12181 aaagttcatt caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat
    12241 gactatcttc ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac
    12301 ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg
    12361 gacgtttgga ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga
    12421 gtttttcttc catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa
    12481 ctgcgccttt tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat
    12541 cataaatata cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat
    12601 caccaactgt gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat
    12661 ttttcatttt ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact
    12721 tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca
    12781 atccaaattg ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg
    12841 cttgttcaat agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact
    12901 tcataatagt agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt
    12961 ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa
    13021 agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt
    13081 cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca
    13141 ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct
    13201 ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt
    13261 ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt
    13321 gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt
    13381 tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt
    13441 cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg
    13501 gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt
    13561 tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta
    13621 cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct
    13681 ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt
    13741 tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg
    13801 cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt
    13861 ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca
    13921 aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg
    13981 atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt
    14041 ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca
    14101 gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc
    14161 tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt
    14221 ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt
    14281 actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc
    14341 aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga
    14401 gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc
    14461 actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca
    14521 ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg
    14581 atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct
    14641 aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt
    14701 aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg
    14761 acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc
    14821 atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt
    14881 ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca
    14941 gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga
    15001 ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa
    15061 tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga
    15121 cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga
    15181 cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca
    15241 tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc
    15301 acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact
    15361 cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt
    15421 tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc
    15481 actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct
    15541 tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct
    15601 cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta
    15661 tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt
    15721 ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt
    15781 ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct
    15841 gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga
    15901 taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag
    15961 ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt
    16021 ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc
    16081 tttgactaat attgacctta attataacga atttgataat agaaagaaag aagaacgctt
    16141 tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACCQP010000057        16011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCQP010000057 DACCQP010000000
VERSION     DACCQP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.059"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IVO87_001994"
     CDS             226..894
                     /locus_tag="IVO87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7450164.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IVO87_001995"
     CDS             complement(1094..1480)
                     /locus_tag="IVO87_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450165.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IVO87_001996"
     CDS             complement(1452..2834)
                     /locus_tag="IVO87_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7450166.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IVO87_001997"
     CDS             complement(2990..4177)
                     /locus_tag="IVO87_001997"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7450167.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IVO87_001998"
     CDS             complement(4184..5215)
                     /locus_tag="IVO87_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7450168.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IVO87_001999"
     CDS             complement(5231..6685)
                     /locus_tag="IVO87_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7450169.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="IVO87_002000"
     CDS             6860..7765
                     /locus_tag="IVO87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7450170.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            7856..8533
                     /locus_tag="IVO87_002001"
     CDS             7856..8533
                     /locus_tag="IVO87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7450171.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IVO87_002002"
     CDS             8814..9710
                     /locus_tag="IVO87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7450172.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IVO87_002003"
     CDS             complement(9765..11135)
                     /locus_tag="IVO87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7450173.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IVO87_002004"
     CDS             11373..12119
                     /locus_tag="IVO87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7450174.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IVO87_002005"
     CDS             12116..12955
                     /locus_tag="IVO87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7450175.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IVO87_002006"
     CDS             12956..13906
                     /locus_tag="IVO87_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7450176.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IVO87_002007"
     CDS             14047..14799
                     /locus_tag="IVO87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450177.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IVO87_002008"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IVO87_002008"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7450178.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5195 a         2999 c         3223 g         4594 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccacagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg t
//
LOCUS       DACCQP010000058        15914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCQP010000058 DACCQP010000000
VERSION     DACCQP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.060"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(148..450)
                     /locus_tag="IVO87_002009"
     CDS             complement(148..450)
                     /locus_tag="IVO87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450179.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IVO87_002010"
     tmRNA           complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IVO87_002010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1207..1980)
                     /locus_tag="IVO87_002011"
     CDS             complement(1207..1980)
                     /locus_tag="IVO87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7450180.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2124..2474
                     /locus_tag="IVO87_002012"
     CDS             2124..2474
                     /locus_tag="IVO87_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7450181.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2455..3171
                     /locus_tag="IVO87_002013"
     CDS             2455..3171
                     /locus_tag="IVO87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450182.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3171..4619
                     /locus_tag="IVO87_002014"
     CDS             3171..4619
                     /locus_tag="IVO87_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7450183.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4690..5199)
                     /locus_tag="IVO87_002015"
     CDS             complement(4690..5199)
                     /locus_tag="IVO87_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450184.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5189..5914)
                     /locus_tag="IVO87_002016"
     CDS             complement(5189..5914)
                     /locus_tag="IVO87_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450185.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6137..8257)
                     /locus_tag="IVO87_002017"
     CDS             complement(6137..8257)
                     /locus_tag="IVO87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7450186.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8487..9665
                     /locus_tag="IVO87_002018"
     CDS             8487..9665
                     /locus_tag="IVO87_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7450187.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9681..10439
                     /gene="aroD"
                     /locus_tag="IVO87_002019"
     CDS             9681..10439
                     /gene="aroD"
                     /locus_tag="IVO87_002019"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7450188.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10462..10947
                     /gene="ybaK"
                     /locus_tag="IVO87_002020"
     CDS             10462..10947
                     /gene="ybaK"
                     /locus_tag="IVO87_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7450189.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11018..12271)
                     /locus_tag="IVO87_002021"
     CDS             complement(11018..12271)
                     /locus_tag="IVO87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7450190.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12388..13737
                     /gene="gdhA"
                     /locus_tag="IVO87_002022"
     CDS             12388..13737
                     /gene="gdhA"
                     /locus_tag="IVO87_002022"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7450191.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13849..15195
                     /locus_tag="IVO87_002023"
     CDS             13849..15195
                     /locus_tag="IVO87_002023"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7450192.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15503..15889)
                     /locus_tag="IVO87_002024"
     CDS             complement(15503..15889)
                     /locus_tag="IVO87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7450193.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5208 a         2843 c         3187 g         4676 t
ORIGIN      
        1 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
       61 acaatcttaa tactaactta aaaaatatcc cttttataag tatggtgata aaatccgttc
      121 cgggctacct tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      181 atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat cagtcacgat
      241 atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa ttttaaagta
      301 gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta aaaagaaaac
      361 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      421 ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact
      481 aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa
      541 aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg
      601 aaaataaaaa atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct
      661 gaaatctcac aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
      721 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
      781 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
      841 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
      901 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
      961 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
     1021 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
     1081 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
     1141 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
     1201 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
     1261 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
     1321 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
     1381 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
     1441 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
     1501 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
     1561 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
     1621 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
     1681 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
     1741 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
     1801 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
     1861 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
     1921 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
     1981 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
     2041 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
     2101 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
     2161 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
     2221 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
     2281 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
     2341 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
     2401 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
     2461 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
     2521 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
     2581 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
     2641 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
     2701 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
     2761 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
     2821 ttggcagaag caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt
     2881 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
     2941 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
     3001 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
     3061 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
     3121 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
     3181 tgtatcagca actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag
     3241 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
     3301 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
     3361 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
     3421 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
     3481 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
     3541 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
     3601 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
     3661 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
     3721 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
     3781 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
     3841 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
     3901 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
     3961 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
     4021 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
     4081 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
     4141 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
     4201 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
     4261 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
     4321 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
     4381 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
     4441 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
     4501 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
     4561 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
     4621 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa
     4681 atgattgatt tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc
     4741 atcatgagta aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg
     4801 gagtagctct tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt
     4861 gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa
     4921 ctcagctgcc gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa
     4981 acgatcttgt ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag
     5041 ataaggaaac atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag
     5101 aacctctttt ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac
     5161 ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt
     5221 gcaaatgatg gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga
     5281 gcccaataag agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga
     5341 aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca
     5401 agatatcacg gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa
     5461 gaagcgcttc ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa
     5521 gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac
     5581 cagtcaggtg ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa
     5641 agataaaaag gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc
     5701 tagctaattc taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc
     5761 gatcttggac ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca
     5821 ataaaaaagc agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa
     5881 ttaatcgagc agtagcatag cggattattt tcatactttt cacctctata aaaagtatag
     5941 aaaatgaata tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa
     6001 atattttgta aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca
     6061 agcttttgaa cactgtttat tcgaaaagag gagcagcgac aataattttg tcgctgctcc
     6121 ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg
     6181 tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag acactagttg
     6241 acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc ttgtagtcat
     6301 aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga agatcaccat
     6361 tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt gaatcaattt
     6421 cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta ctatagatcg
     6481 taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa gaaacaagct
     6541 gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt gtatctacac
     6601 ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg tgattgattc
     6661 cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt tgtgcattgt
     6721 cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt ttatttcttg
     6781 aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca tacaagccgc
     6841 tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg gctgtcgcaa
     6901 agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct tcgtcattgg
     6961 tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa aatggttgtt
     7021 gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc aacccgtatt
     7081 ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa tcatatccta
     7141 aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg aataccgcac
     7201 tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta ttttttccgc
     7261 ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc atcagcgttt
     7321 ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc
     7381 tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga ccattctcat
     7441 ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata taaatcacat
     7501 tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt tcttttacat
     7561 aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg tttgtctggt
     7621 aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg accaaataat
     7681 cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata aaaagatttc
     7741 ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct cggatcgggc
     7801 gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata aaatcgatcc
     7861 aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga ccactcgcta
     7921 ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct cgataataag
     7981 tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc gtgatataag
     8041 caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta gttgcaatag
     8101 ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg aaatcaacta
     8161 aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa aatccgagcc
     8221 tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt tctgcacttc
     8281 ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca tttattttac
     8341 tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat taaggttttc
     8401 tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt tatacttggg
     8461 aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc
     8521 gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc caaaaagaaa
     8581 ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag
     8641 gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca tttgatcaat
     8701 ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg
     8761 attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc ggtctgattg
     8821 ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta tatgcacatc
     8881 gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga ggctatgaga
     8941 aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga aaagaggcac
     9001 cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta aatgaaggat
     9061 taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca gaaggtttcg
     9121 cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct gttgctgccg
     9181 caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa
     9241 cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc attgttatgg
     9301 acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac atgattgtat
     9361 tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca aaagattatg
     9421 gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt attgcgtcta
     9481 caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag gaattccatg
     9541 ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat ttccagacga
     9601 cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt aaaaataacc
     9661 attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga agcggtagac
     9721 caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt gaagcagttc
     9781 ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat cgtgaagttt
     9841 tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt gatggactgc
     9901 cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata tcgatccgtc
     9961 agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg gtcgatatcg
    10021 agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag tctttaaaca
    10081 ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct gtcttgcttt
    10141 accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca gtaactccca
    10201 ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat gcttttgtct
    10261 ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt ctggttggcg
    10321 gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact tctgctccag
    10381 gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga
    10441 acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt
    10501 atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga cgaaagccac
    10561 ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat atttaaaaca
    10621 ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg agaagccgaa
    10681 ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat gctccattta
    10741 aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat aggtatgaaa
    10801 aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat cattgtctcg
    10861 gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc attaacgagc
    10921 ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag gaggccggga
    10981 tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc ccggcattca
    11041 ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca gtataaccat
    11101 tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc tccaatgttt
    11161 taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc tcaataggaa
    11221 aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct tgaatttgtt
    11281 tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga aagaaaacta
    11341 acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg gttgctttac
    11401 ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa caatccagag
    11461 aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt gaacaacgca
    11521 gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca
    11581 atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa
    11641 gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc gcataaagag
    11701 gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta gaaccagtag
    11761 atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac tcttcgaatt
    11821 ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact cgagtagaga
    11881 atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata atcgtttttg
    11941 atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt acgccttgtg
    12001 attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta tataaaaact
    12061 ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc gtaacattgc
    12121 accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt tcgatcaaga
    12181 gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt gttccagcgc
    12241 cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag tgtagcatat
    12301 ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
    12361 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
    12421 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
    12481 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
    12541 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
    12601 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
    12661 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
    12721 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
    12781 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
    12841 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
    12901 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
    12961 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
    13021 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
    13081 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
    13141 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
    13201 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
    13261 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
    13321 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
    13381 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
    13441 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
    13501 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgtccagcga
    13561 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
    13621 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
    13681 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
    13741 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
    13801 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
    13861 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
    13921 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
    13981 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
    14041 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
    14101 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
    14161 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
    14221 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
    14281 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
    14341 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
    14401 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
    14461 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
    14521 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
    14581 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
    14641 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
    14701 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
    14761 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
    14821 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
    14881 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
    14941 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
    15001 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
    15061 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
    15121 aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc taaagaattg
    15181 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
    15241 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
    15301 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
    15361 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
    15421 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
    15481 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
    15541 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
    15601 agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta atgacatcga
    15661 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
    15721 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
    15781 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
    15841 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
    15901 ttttttattt ttat
//
LOCUS       DACCQP010000059        15128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCQP010000059 DACCQP010000000
VERSION     DACCQP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.061"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IVO87_002025"
     CDS             278..787
                     /locus_tag="IVO87_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7450194.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVO87_002026"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVO87_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7450195.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVO87_002027"
     CDS             2322..3302
                     /locus_tag="IVO87_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7450196.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IVO87_002028"
     CDS             3424..4842
                     /locus_tag="IVO87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7450197.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IVO87_002029"
     CDS             4913..6382
                     /locus_tag="IVO87_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7450198.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLTNRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IVO87_002030"
     CDS             6392..6859
                     /locus_tag="IVO87_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7450199.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IVO87_002031"
     CDS             6895..7767
                     /locus_tag="IVO87_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7450200.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IVO87_002032"
     CDS             7848..10382
                     /locus_tag="IVO87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7450201.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IVO87_002033"
     CDS             10538..11311
                     /locus_tag="IVO87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7450202.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IVO87_002034"
     CDS             11304..12095
                     /locus_tag="IVO87_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7450203.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IVO87_002035"
     CDS             12105..12596
                     /locus_tag="IVO87_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7450204.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IVO87_002036"
     CDS             12614..13024
                     /locus_tag="IVO87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7450205.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IVO87_002037"
     CDS             13028..14428
                     /locus_tag="IVO87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP7450206.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14830
                     /locus_tag="IVO87_002038"
                     /pseudo
     CDS             14451..14830
                     /locus_tag="IVO87_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         5258 a         2124 c         3026 g         4720 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctgt ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactaaccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaat
    14701 tcaagattta ggatatgaat tattagataa aaatattatg gaattaccgt tctgggaaaa
    14761 tggaatccgt tactttaatt tctacggacc aaatcatgag attatagaga taagccaaat
    14821 gaagaaataa atagataacg aagtgctcta agttggtatg aacgaaaagt acttcatttt
    14881 ttggtatcag catttcaata gtttaatgag aatttactat ttcaaattaa ttcaaagtga
    14941 cagtattttt tacttatttg gtttcaaatt ttgtaatcct tcctatttat tggaatacac
    15001 tccacatttt gtatacaagt catatcatgt tttagcaaag ctcttaccaa gcaaagttat
    15061 aatgatgttt tccaaagttt gatctttcga tggaggctaa cacatatttt ttgcaggaca
    15121 gccaaaat
//
LOCUS       DACCQP010000060        14888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCQP010000060 DACCQP010000000
VERSION     DACCQP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.062"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(73..1809)
                     /locus_tag="IVO87_002039"
     CDS             complement(73..1809)
                     /locus_tag="IVO87_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7450207.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1806..2510)
                     /locus_tag="IVO87_002040"
     CDS             complement(1806..2510)
                     /locus_tag="IVO87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450208.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2641..3144
                     /locus_tag="IVO87_002041"
     CDS             2641..3144
                     /locus_tag="IVO87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7450209.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3104..3442
                     /locus_tag="IVO87_002042"
     CDS             3104..3442
                     /locus_tag="IVO87_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7450210.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3463..4881
                     /gene="ffh"
                     /locus_tag="IVO87_002043"
     CDS             3463..4881
                     /gene="ffh"
                     /locus_tag="IVO87_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7450211.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4993..5571
                     /locus_tag="IVO87_002044"
     CDS             4993..5571
                     /locus_tag="IVO87_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7450212.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5575..6096
                     /locus_tag="IVO87_002045"
     CDS             5575..6096
                     /locus_tag="IVO87_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7450213.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6175..6729)
                     /locus_tag="IVO87_002046"
     CDS             complement(6175..6729)
                     /locus_tag="IVO87_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7450214.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6982..7257
                     /gene="rpsP"
                     /locus_tag="IVO87_002047"
     CDS             6982..7257
                     /gene="rpsP"
                     /locus_tag="IVO87_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7450215.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7269..7520
                     /locus_tag="IVO87_002048"
     CDS             7269..7520
                     /locus_tag="IVO87_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7450216.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7645..8436
                     /locus_tag="IVO87_002049"
     CDS             7645..8436
                     /locus_tag="IVO87_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450217.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8606..9127
                     /gene="rimM"
                     /locus_tag="IVO87_002050"
     CDS             8606..9127
                     /gene="rimM"
                     /locus_tag="IVO87_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7450218.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9117..9878
                     /gene="trmD"
                     /locus_tag="IVO87_002051"
     CDS             9117..9878
                     /gene="trmD"
                     /locus_tag="IVO87_002051"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7450219.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10014..10361
                     /gene="rplS"
                     /locus_tag="IVO87_002052"
     CDS             10014..10361
                     /gene="rplS"
                     /locus_tag="IVO87_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7450220.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10493..11629)
                     /locus_tag="IVO87_002053"
     CDS             complement(10493..11629)
                     /locus_tag="IVO87_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7450221.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11746..12438)
                     /locus_tag="IVO87_002054"
     CDS             complement(11746..12438)
                     /locus_tag="IVO87_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450222.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(12576..13229)
                     /locus_tag="IVO87_002055"
     CDS             complement(12576..13229)
                     /locus_tag="IVO87_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450223.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(13279..13707)
                     /locus_tag="IVO87_002056"
     CDS             complement(13279..13707)
                     /locus_tag="IVO87_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7450224.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(13712..14086)
                     /locus_tag="IVO87_002057"
     CDS             complement(13712..14086)
                     /locus_tag="IVO87_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450225.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            14371..14556
                     /locus_tag="IVO87_002058"
     CDS             14371..14556
                     /locus_tag="IVO87_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450226.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14569..14817
                     /locus_tag="IVO87_002059"
     CDS             14569..14817
                     /locus_tag="IVO87_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450227.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
BASE COUNT         4753 a         2673 c         2906 g         4556 t
ORIGIN      
        1 aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa acgcacgagc
       61 agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg
      121 actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa tcgctaaccc
      181 taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa
      241 gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag aaaacgatag
      301 atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat attggacagc
      361 attttcaatt agatttttga taattggata aaggatatct gttttagtag taaatggttc
      421 gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct gataattttg
      481 aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag aattctcgcc
      541 atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg catctttttg
      601 catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat ccagtaaagt
      661 ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga cgttgccaac
      721 gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg ccacccctag
      781 aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg
      841 accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca cttgatgaat
      901 cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag gttgatagct
      961 taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag aatcagcatc
     1021 atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt gctcttctgt
     1081 ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa tcgtctgata
     1141 caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag gattttgcac
     1201 catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat agatcagata
     1261 atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga cccaacggcg
     1321 aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta aaatggcttc
     1381 taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat tcaaagtagt
     1441 acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga tttcgggacg
     1501 ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct ttccagtagc
     1561 atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag cagttttgtt
     1621 ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg ctttttttgt
     1681 caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca
     1741 gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat aaattttttt
     1801 ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt caccaagtac
     1861 tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg tacatctacg
     1921 attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg
     1981 atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt cggtgttagt
     2041 tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt gatttgtcca
     2101 atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc aattcgtctg
     2161 aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt caaatagtcg
     2221 tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc cgtcagcatc
     2281 aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat gccatccatc
     2341 gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc taattctaat
     2401 ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa attgaacgtc
     2461 aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat tcaaatctcc
     2521 tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag ggctatttta
     2581 caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga agagcagtca
     2641 atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa
     2701 gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt ctgtccagaa
     2761 gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg aaatggattc
     2821 gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg
     2881 tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat tgatctagtc
     2941 atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg aagtttttcc
     3001 ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca aaaaatgact
     3061 gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa tcgaaaagac
     3121 caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa
     3181 ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg aagaatacga
     3241 agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct tggaggatta
     3301 tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat tggaaaatat
     3361 gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat atgtacagca
     3421 aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt tgaaagctta
     3481 actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct
     3541 gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga tgttaacttg
     3601 caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg
     3661 gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt gacgatcaca
     3721 cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt tatcatgatg
     3781 gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa ttatttaaag
     3841 aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc agctgcgatc
     3901 gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat gggaactgaa
     3961 gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac
     4021 tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat ggatgagttg
     4081 aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga tgccatgact
     4141 ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat tacaggggtc
     4201 gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat ccgctcagtg
     4261 acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt agaagtattc
     4321 cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac attgattgaa
     4381 aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa
     4441 aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg tatgggccca
     4501 ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat cgaaaacgtc
     4561 aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat gacgccagca
     4621 gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc tgccggttct
     4681 ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg
     4741 atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat gtttggttca
     4801 ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa aaataagaag
     4861 aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg aaaatgtcta
     4921 ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac tctaaacgaa
     4981 gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg gcaatatcat
     5041 gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa attgatgctt
     5101 gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga agcattcgat
     5161 gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt
     5221 gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc agccgtccaa
     5281 aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga gtatctttgg
     5341 tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga agtcccaaca
     5401 acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt taaatttatc
     5461 ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga tcaccttgac
     5521 agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata aagcatgcga
     5581 aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa aacggatgat
     5641 cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc tttttatgaa
     5701 acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa atatttggag
     5761 accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc acaattacaa
     5821 ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg
     5881 acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa
     5941 catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg ggacgggatt
     6001 cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac gacacgattc
     6061 ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata aaaaaggcaa
     6121 ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg ctttttactc
     6181 tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga aacttaatgt
     6241 tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga tatatgcttc
     6301 cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc
     6361 gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt tatgatccca
     6421 aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata ctgctggcac
     6481 agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca cttcctcacg
     6541 aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat tgtaaaatgg
     6601 cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt tcgcaaaagc
     6661 ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa atccaatttt
     6721 ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa aagataagta
     6781 actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat tacttatttt
     6841 taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt aatcaagctg
     6901 taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt gtgaataaaa
     6961 caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat gggttctaaa
     7021 aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc
     7081 atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt tttaaaagaa
     7141 gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt acgtaacatc
     7201 ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa gaaataaggt
     7261 gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg ctagtcactt
     7321 atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag tacaatctaa
     7381 cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa
     7441 tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca
     7501 tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt
     7561 gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt
     7621 ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta
     7681 ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt
     7741 atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc caccactgaa
     7801 caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa
     7861 cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca
     7921 gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg
     7981 ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg
     8041 tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga cacaaatggt
     8101 cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt
     8161 tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc
     8221 caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca
     8281 gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta
     8341 gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct
     8401 gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca
     8461 tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt
     8521 ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca
     8581 gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa
     8641 cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg
     8701 ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg tggaattaac
     8761 agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc
     8821 tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt
     8881 agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga
     8941 tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt
     9001 cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga
     9061 agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga
     9121 agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca
     9181 tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt
     9241 cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc
     9301 tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca
     9361 aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag
     9421 aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac
     9481 gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag
     9541 aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga
     9601 atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata
     9661 ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata
     9721 aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg
     9781 atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga
     9841 aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tcttctttac
     9901 agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat
     9961 attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc
    10021 cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg
    10081 gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat
    10141 ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta
    10201 aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa
    10261 aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat
    10321 tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcattgtta tcagactgag
    10381 aaagaaccct tggtattcaa gggttctttt tttattatct gtttttttat attaacgatt
    10441 tctatttggt acatttttga ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt
    10501 tttctatatc ttctttcaat ttttctttta ctccttttgt cacatgcaaa taaatctttt
    10561 cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga ggagttccta
    10621 gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt tttttatcaa
    10681 tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt tggaaaggag
    10741 ttcctttcgt tgtttgaaat agaaattgac ccttcggatt taattctaga agctcattgt
    10801 aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca gtttttgtag
    10861 aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc ccatttataa
    10921 ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg attgcttctc
    10981 caggtctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt ttattgtgtg
    11041 aagttgtgaa atctactagt cttttatatt catcatcttc taaaaactta tctttaattt
    11101 ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga tttttttcta
    11161 catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta attacactga
    11221 cgtacttatt tgacaagtca tctttatata tcatatcttc aaaaatatta tttaaatcta
    11281 ctgtattaat accagaaacg atgtaagtct caggcatctt cttctttata gtgtttaaga
    11341 tattattcgt aggataatat gtagactcct taacttgcct tttgtaaata actaaccatt
    11401 cttcgacaag ctcatgaaat gttagattag gcttctggag tgttttttca tttagtttta
    11461 tatcaatttt tttatttaac tccagtaatg ccactttctg agtttctcga gacttatttt
    11521 tataagtaat gctcactttt tttctttttc tagtttttgg atcaatatat cgttcattat
    11581 atttatatac tttttttccg tttttatcag ttttggtttc aatccacatt gttttcatct
    11641 cctatttgtt gctataatag gaatggataa gtaagccgat tatagcaggt ttatttttca
    11701 tcacgtccac aaactttggc gagggagggg gcgtgttttt gtattttaac gatttatatt
    11761 gaatgttgat tcagcacttt cagcaactgt actataatca gacggattat ataacactcc
    11821 ggtttttatc gttacttttg taatatcgtt aactttagat ggatctaagt aagctaaact
    11881 ataaaattct ttactttcag tttttttgaa aaacgagttt gttgtcattg tatgcataag
    11941 atcgtctgaa ctgaaatcaa tttgttggcc gttatcaagt atggcgtgtg caagcccatt
    12001 ataaccataa tctacttcag aattgttttt aactttgaat gcaatttgta aaccataaaa
    12061 atttccttca ctatctttgt gattatttgc atcgtagtaa ccagcagcgc tagggtcagc
    12121 ataattggaa tacttgagta gtttgatatt agatatataa atgccgatat caccaagagg
    12181 aatatctgat tgtgggcttg ccgttccaat taattcaatt ttgccacttg tatctctttt
    12241 ttctttggtc cattgaccaa ctttgaggag ggggccatca acctcctgtg aatttttaga
    12301 agactgtgtt gtactttctg ataacgtaac tgaagagtct ttttcgtttg ttttgccagt
    12361 attatctaca acattagatt ggctacatcc ggtaagaaaa aaagcaccaa atactaaccc
    12421 taaaatcact tttttcattc tttattctcc gtttctatga tattattttt atgtaggatc
    12481 ttagaaacga gatcttagtc cgtgttgcag cacgggcttt tttctttata acttttttag
    12541 agattatagg caaaatagta ggacacaaaa atatattatt gtattccgta tttagaaaat
    12601 cctaattgaa cttcaccaga agttttttgc tgtgttgtac gcaatgcttc ttcttcagac
    12661 attccattct gtactttcca tgcaacaggc gacatcccgt atttgttaac aaaatcagta
    12721 agtgataaag tgtcagcgtc ttgctgaccg cttgtttgtt ggtcttctgg attttgttga
    12781 gatgctgctt gttgttcttt ctgatcttga ctgataatat tgccagcatc atctgtagtc
    12841 aatccatttt cataaagggc cacgccgaaa gcttcccact ctttgttgga ccaatttgca
    12901 cgatcagctg gagttgactg taaagtgcgt tgtttcatct gttcatatgt ttcttcttga
    12961 ggtgcggttt ggattgtatc ctgactggag cttataactg ttgggctagg ttccgccgta
    13021 gcttggttag agcttgtaac tgttgaacta gaatctgtct tagatgtaga tttgctagta
    13081 gaggaactgg tttcagttgt ttcttttgtt ttacttactt ttgtttcttg gttagaagtg
    13141 gcatctgttg attcagcttt tttattattt gaacaagctg aaagtagcag agcagtactt
    13201 aacaacaaca taacgctaac ttttttcatt ttataattcc tctttctcgt tgtaatatgt
    13261 gtgctaacac gggctttttt atataagaaa acgataagcg ctttctggaa gtccgtaaag
    13321 attctttaat tcatcgattt ttttgggata ttgatcatta tcttctttat aaagagaaac
    13381 aatgagatta gcagcaaagc aattagcttc gctttcagat ttgcttctag atgttcttgt
    13441 tgatacatag taactggata agccgcgatg aaaaatagcg tgacctaatt cgtgagcgca
    13501 aatgtagaat ctttcctcag agtctcgcag ttcatcattt aagaagatta ttgcacgacc
    13561 tctaatttct tgaaactgtc ctttgggatt ttcgataaaa ggaacgtatt gaattttaat
    13621 gcccatcttt tcacaaatat aaaaaggatt agcagaccgg tatttccgct tcaactcctc
    13681 gactaaatta atcgtatcca tctccataag ctcacatctt tttgcctttt tctttgtctt
    13741 ctttcacaat atcccagaag gttccaataa ggatatcttt tacgcgttgg acctgttcag
    13801 gcgtcagtgt ttctccgcca taagacatat tgacatttga atctagtaat ttatcaagtt
    13861 caactacttc atcttttgtt gcccagtcag gaacttggtt tctaccgagt aaataatcag
    13921 tagttacatt aaaataatct gctacaagtt gtagtctttt agtactagga gttctatttt
    13981 tccattgata aatcgtgttt ttaggtatat ttaattcttc ttccaattga gttatactta
    14041 tgcctctttt atgagctaat tcctttattc tatctagtaa attcattttc gcacctcaca
    14101 agctacgaaa acactaataa aaaaattagt taaaggtgtt gacaactaat aaaattgtta
    14161 gtatactgtt ttcgtaagct aaaatattag ctaaaaagac ttgataacta ataaacactt
    14221 cacggtcggc aaactctgaa atgtaaatta ctaggcattt ctgtgtctta tttagctata
    14281 tctatatact aataaaaata ttagttattg tcaacgaata ttagctaatt ttttagctta
    14341 cagactattt tttagaaagg agctattttt atgtctgaga atttagactt aaaaattcga
    14401 gcggagatga gaaaaagaag aatgactttc aaagaactag ctgcgcttgt tggtatttca
    14461 ggagcttatt tatcagatat tctaaacggc aatcgtgatg gaaagaaagc acaacagcat
    14521 atcgaaacag tgaaaaaaat attggacatc cgataggggg ggtaggcgat gacaaaacta
    14581 aaaaaacaag attttgtaaa aaaatacaat tattctccat ctacttatca acgtcgaatg
    14641 tcggaactaa aaaatacagc aattttctca gcggcgtatg aacgggtcac aggacaagaa
    14701 gtttggatca atacagaatt atacgataaa tttttgtctt tcaaatccta taacaggtta
    14761 cgcacaagaa agataacacc taaagaattt atcgagaagc atttagttga tttataaaaa
    14821 acagaacatt ttgagaggtg aaggttaatg ggcaaattca acagagcact agtattcagc
    14881 acaccatt
//
LOCUS       DACCQP010000061        14727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCQP010000061 DACCQP010000000
VERSION     DACCQP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.063"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(537..899)
                     /locus_tag="IVO87_002060"
     CDS             complement(537..899)
                     /locus_tag="IVO87_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450228.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(973..1353)
                     /locus_tag="IVO87_002061"
     CDS             complement(973..1353)
                     /locus_tag="IVO87_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7450229.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1435..2610
                     /locus_tag="IVO87_002062"
     CDS             1435..2610
                     /locus_tag="IVO87_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7450230.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2686..3270)
                     /locus_tag="IVO87_002063"
     CDS             complement(2686..3270)
                     /locus_tag="IVO87_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7450231.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3471..4469
                     /locus_tag="IVO87_002064"
     CDS             3471..4469
                     /locus_tag="IVO87_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7450232.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4581..5090
                     /locus_tag="IVO87_002065"
     CDS             4581..5090
                     /locus_tag="IVO87_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7450233.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5120..5779)
                     /locus_tag="IVO87_002066"
     CDS             complement(5120..5779)
                     /locus_tag="IVO87_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7450234.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5781..6545)
                     /locus_tag="IVO87_002067"
     CDS             complement(5781..6545)
                     /locus_tag="IVO87_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7450235.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6553..7230)
                     /locus_tag="IVO87_002068"
     CDS             complement(6553..7230)
                     /locus_tag="IVO87_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7450236.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7267..8652)
                     /locus_tag="IVO87_002069"
     CDS             complement(7267..8652)
                     /locus_tag="IVO87_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7450237.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8862..8946)
                     /gene="ffs"
                     /locus_tag="IVO87_002070"
     ncRNA           complement(8862..8946)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVO87_002070"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8969..9484)
                     /gene="tadA"
                     /locus_tag="IVO87_002071"
     CDS             complement(8969..9484)
                     /gene="tadA"
                     /locus_tag="IVO87_002071"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7450238.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9591..10448
                     /locus_tag="IVO87_002072"
     CDS             9591..10448
                     /locus_tag="IVO87_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7450239.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10529..10864)
                     /locus_tag="IVO87_002073"
     CDS             complement(10529..10864)
                     /locus_tag="IVO87_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450240.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10931..12301)
                     /locus_tag="IVO87_002074"
     CDS             complement(10931..12301)
                     /locus_tag="IVO87_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7450241.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12379..12915)
                     /locus_tag="IVO87_002075"
     CDS             complement(12379..12915)
                     /locus_tag="IVO87_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7450242.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13224..14705
                     /locus_tag="IVO87_002076"
     CDS             13224..14705
                     /locus_tag="IVO87_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7450243.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4525 a         2923 c         2391 g         4888 t
ORIGIN      
        1 cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca agcgattgtc
       61 aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc aactcattta
      121 tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc gatagaatca
      181 agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt tagtaacatg
      241 tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg
      301 ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga
      361 aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa
      421 ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac
      481 aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag
      541 aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct
      601 tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca
      661 aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg
      721 ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt
      781 ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat
      841 tttttagtac catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa
      901 atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa
      961 aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat
     1021 ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa
     1081 ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa
     1141 actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac
     1201 ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag
     1261 tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt
     1321 tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat
     1381 tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc
     1441 gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct
     1501 gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct
     1561 cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc
     1621 actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg
     1681 gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca
     1741 gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca
     1801 ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa
     1861 aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta
     1921 aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa
     1981 ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc
     2041 catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat
     2101 ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc
     2161 aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta
     2221 gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat
     2281 gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca
     2341 gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact
     2401 tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact
     2461 cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat
     2521 gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga
     2581 atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct
     2641 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2701 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2761 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2821 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2881 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2941 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     3001 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     3061 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     3121 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     3181 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     3241 actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca
     3301 taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt
     3361 tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga
     3421 gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct
     3481 atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa
     3541 tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa
     3601 ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg
     3661 aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag
     3721 aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca
     3781 tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat
     3841 tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt
     3901 tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt
     3961 ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat
     4021 ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc
     4081 cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa
     4141 aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat
     4201 tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta
     4261 ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta
     4321 cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga
     4381 acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct
     4441 tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa
     4501 cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa
     4561 aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag
     4621 aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac
     4681 aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc
     4741 taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag
     4801 aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg
     4861 gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga
     4921 attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg
     4981 gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca
     5041 gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa
     5101 aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt
     5161 ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc
     5221 tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata
     5281 accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta
     5341 tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca
     5401 accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag
     5461 tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg
     5521 ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga
     5581 gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat
     5641 ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac
     5701 caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact
     5761 gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta
     5821 gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa
     5881 gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg
     5941 attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct
     6001 ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac
     6061 cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg
     6121 aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa
     6181 ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct
     6241 aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct
     6301 gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga
     6361 atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg
     6421 gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc
     6481 ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag
     6541 tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac
     6601 ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg
     6661 atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata
     6721 cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc
     6781 gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc
     6841 caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt
     6901 taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc
     6961 actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg
     7021 ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt
     7081 gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt
     7141 gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac
     7201 gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc
     7261 ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa
     7321 ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa
     7381 catcaaatga tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt
     7441 ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa
     7501 aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt
     7561 ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg
     7621 catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc
     7681 agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc
     7741 ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg
     7801 caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat
     7861 ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg
     7921 cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta
     7981 ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg
     8041 ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc
     8101 ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac
     8161 taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag
     8221 cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat
     8281 tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag
     8341 gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac
     8401 gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt
     8461 ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc
     8521 tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa
     8581 ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac
     8641 tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt
     8701 cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt
     8761 acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat
     8821 aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc
     8881 ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc
     8941 ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta
     9001 atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt
     9061 cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat
     9121 tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta
     9181 cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg
     9241 cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt
     9301 ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt
     9361 ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg
     9421 cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta
     9481 ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc
     9541 tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg
     9601 ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg
     9661 atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc
     9721 aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc
     9781 catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga
     9841 tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt
     9901 tggaattatt tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag
     9961 tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt
    10021 tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca
    10081 ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc
    10141 tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag
    10201 atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa
    10261 tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa
    10321 cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg
    10381 gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac
    10441 ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga
    10501 atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc
    10561 ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt
    10621 ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac
    10681 catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta
    10741 ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg
    10801 ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt
    10861 tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga
    10921 actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta
    10981 cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc
    11041 catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca
    11101 agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc
    11161 gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag
    11221 aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag
    11281 cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt
    11341 gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag
    11401 tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt
    11461 tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag
    11521 gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt
    11581 cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta
    11641 ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc
    11701 actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa
    11761 ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg
    11821 tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt
    11881 tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg
    11941 ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag
    12001 cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga
    12061 ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta
    12121 attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc
    12181 gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc
    12241 ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca
    12301 ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct
    12361 actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc
    12421 cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc
    12481 atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt
    12541 gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc
    12601 taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa
    12661 taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata
    12721 aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata
    12781 aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat
    12841 tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt
    12901 tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata aataacataa
    12961 ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat
    13021 caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata
    13081 gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg
    13141 tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa
    13201 aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc
    13261 tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata
    13321 ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt
    13381 tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg
    13441 atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca
    13501 atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat
    13561 atttacttga acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca
    13621 atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg
    13681 aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag
    13741 gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg
    13801 agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca
    13861 tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga
    13921 tggacttatt gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc
    13981 gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc tattgttttt
    14041 ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca
    14101 aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc
    14161 tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt
    14221 ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca
    14281 cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa
    14341 ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac
    14401 acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt
    14461 ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta
    14521 aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt
    14581 cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt
    14641 taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga
    14701 tgtgacaaaa ttcttgtcac atcctct
//
LOCUS       DACCQP010000062        14639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCQP010000062 DACCQP010000000
VERSION     DACCQP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.064"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IVO87_002077"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IVO87_002077"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7450244.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IVO87_002078"
     CDS             951..1256
                     /locus_tag="IVO87_002078"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7450245.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IVO87_002079"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IVO87_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7450246.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IVO87_002080"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IVO87_002080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7450247.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IVO87_002081"
     CDS             4416..5354
                     /locus_tag="IVO87_002081"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7450248.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IVO87_002082"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IVO87_002082"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7450249.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IVO87_002083"
     CDS             6725..7465
                     /locus_tag="IVO87_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7450250.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IVO87_002084"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IVO87_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7450251.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IVO87_002085"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IVO87_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7450252.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IVO87_002086"
     CDS             10608..11258
                     /locus_tag="IVO87_002086"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7450253.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IVO87_002087"
     CDS             11259..11897
                     /locus_tag="IVO87_002087"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7450254.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IVO87_002088"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IVO87_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7450255.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IVO87_002089"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IVO87_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7450256.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..>14639
                     /locus_tag="IVO87_002090"
     CDS             13674..>14639
                     /locus_tag="IVO87_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450257.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEE"
BASE COUNT         4918 a         2716 c         3184 g         3821 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaa
//
LOCUS       DACCQP010000063        14549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCQP010000063 DACCQP010000000
VERSION     DACCQP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.065"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IVO87_002091"
     CDS             4..816
                     /locus_tag="IVO87_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7450258.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IVO87_002092"
     CDS             813..1613
                     /locus_tag="IVO87_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7450259.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IVO87_002093"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IVO87_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7450260.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IVO87_002094"
     CDS             3110..4819
                     /locus_tag="IVO87_002094"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7450261.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IVO87_002095"
     CDS             5031..9389
                     /locus_tag="IVO87_002095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7450262.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IVO87_002096"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IVO87_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7450263.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IVO87_002097"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IVO87_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7450264.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IVO87_002098"
     CDS             11227..11520
                     /locus_tag="IVO87_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7450265.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IVO87_002099"
     CDS             11517..11828
                     /locus_tag="IVO87_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7450266.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IVO87_002100"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IVO87_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7450267.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IVO87_002101"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IVO87_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7450268.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2581 c         3236 g         3773 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttag ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DACCQP010000064        14525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCQP010000064 DACCQP010000000
VERSION     DACCQP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.066"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IVO87_002102"
     CDS             <1..197
                     /locus_tag="IVO87_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450269.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IVO87_002103"
     CDS             314..811
                     /locus_tag="IVO87_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7450270.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IVO87_002104"
     CDS             898..1290
                     /locus_tag="IVO87_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450271.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IVO87_002105"
     CDS             1274..3721
                     /locus_tag="IVO87_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450272.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IVO87_002106"
     CDS             3724..5907
                     /locus_tag="IVO87_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7450273.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IVO87_002107"
     CDS             5904..6905
                     /locus_tag="IVO87_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7450274.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IVO87_002108"
     CDS             6920..7834
                     /locus_tag="IVO87_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450275.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IVO87_002109"
     CDS             8079..8195
                     /locus_tag="IVO87_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7450276.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8211..10130
                     /gene="tet(M)"
                     /locus_tag="IVO87_002110"
     CDS             8211..10130
                     /gene="tet(M)"
                     /locus_tag="IVO87_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7450277.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10228..11601
                     /gene="tet(L)"
                     /locus_tag="IVO87_002111"
     CDS             10228..11601
                     /gene="tet(L)"
                     /locus_tag="IVO87_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7450278.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            12165..13427
                     /locus_tag="IVO87_002112"
     CDS             12165..13427
                     /locus_tag="IVO87_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7450279.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            13672..>14525
                     /locus_tag="IVO87_002113"
     CDS             13672..>14525
                     /locus_tag="IVO87_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7450280.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         4742 a         2464 c         3048 g         4271 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
     8281 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
     8341 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
     8401 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
     8461 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
     8521 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
     8581 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
     8641 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
     8701 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
     8761 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
     8821 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
     8881 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
     8941 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
     9001 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
     9061 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     9121 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     9181 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     9241 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     9301 cacttttaga aatctccgac agtgacccgc ttctacaata ttatgtggat tctacgacac
     9361 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
     9421 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
     9481 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
     9541 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
     9601 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
     9661 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtatg
     9721 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
     9781 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
     9841 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
     9901 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
     9961 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
    10021 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
    10081 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
    10141 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
    10201 aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag ggttaaatac
    10261 aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt
    10321 ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc gagtacaaac
    10381 tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata tggaaagcta
    10441 tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa ttgtttcggg
    10501 tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc tcgttttatt
    10561 caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc gcgctatatt
    10621 ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc catgggagaa
    10681 ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc ctatcttcta
    10741 ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt aaagaaagaa
    10801 gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt aggcattgta
    10861 ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag cgtgctgtca
    10921 ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc cttttgttga tcccggatta
    10981 gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt tggaacagta
    11041 gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct aagtactgcc
    11101 gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt cggctacatt
    11161 ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg agttacattt
    11221 ctttctgtta gctttttaac tgcttccttt cttttagaaa caacatcatg gttcatgaca
    11281 attataatcg tatttgtttt aggtgggctt tcgttcacca aaacagttat atcaacaatt
    11341 gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa tgagtttgct taactttacc
    11401 agctttttat cagagggaac aggtattgca attgtaggtg gtttattatc cataccctta
    11461 cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa cttatctgta tagtaatttg
    11521 ttattacttt tttcaggaat cattgtcatt agttggctgg ttaccttgaa tgtatataaa
    11581 cattctcaaa gggatttcta aatcgttaag ggatcaactt tgggagagag ttcaaaattg
    11641 atcctttttt tataacagga attcaaatct ttttgttcca ttaaagggcg cgattgctga
    11701 ataaaagata cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac
    11761 actagaaaac cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct
    11821 agacaaaacg gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat
    11881 cttctcaaaa aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc
    11941 ccctttgctg aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga
    12001 aaggtcttaa aggttttatg gttttggtcg gcactgccga cagcctcgca gagcacacac
    12061 tttatgaata taaagtatag tgtgttatac tttacttgga agtggttgcc ggaaagagcg
    12121 aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt acatatgagt tatgcagttt
    12181 gtagaatgca aaaagtgaaa tcagctggac taaaaggcat gcaatttcat aatcaaagag
    12241 agcgaaaaag tagaacgaat gatgatattg accatgagcg aacacgtgaa aattatgatt
    12301 tgaaaaatga taaaaatatt gattacaacg aacgtgtcaa agaaattatt gaatcacaaa
    12361 aaacaggtac aagaaaaacg aggaaagatg ctgttcttgt aaatgagttg ctagtaacat
    12421 ctgaccgaga tttttttgag caactggatc caggagaaca aaaacgattt tttgaggaaa
    12481 gttataaatt attttccgaa cgatatggca agcaaaatat tgcttatgca acagttcata
    12541 atgatgagca aacccctcac atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc
    12601 aaggaaaaaa tgtgtttaat cgtcaagaac tgttatggct acaagataaa ttccccgagc
    12661 atatgaaaaa acagggtttt gagttgaagc gtggtgaacg tggctctgac cgtaaacata
    12721 ttgagacagc taaatttaaa aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa
    12781 atttagcagt taaaaaagat gaatggactg cttatagcga taaagttaaa tcagatttag
    12841 aagtaccagc gaaacgacac atgaaaagtg ttgaagtgcc aacgggtgaa aagtccatgt
    12901 ttggtttggg aaaagaaata atgaaaacag aaaagaaacc aaccaaaaat gttgttatat
    12961 cggagcgtga ttataaaaac ttagtgactg ctgcgagaga taacgatagg ttaaaacagc
    13021 atgttagaaa tctcatgagt actgatatgg cgagagaata taaaaaatta agtaaagaac
    13081 atgggcaagt taaagaaaaa tatagtggtc ttgtagagcg atttaatgaa aatgtaaatg
    13141 attataatga gttgcttgaa gaaaacaagt ctttaaagtc taaaataagc gatttaaagc
    13201 gtgatgtgag tttaatctat gaaagcacta aggaattcct taaggaacgt acagacggct
    13261 taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa ggtaaaggat aaaacagcac
    13321 aattccaaga aaaacacgat ttagaaccta aaaagaacga atttgaacta actcataacc
    13381 gagaggtaaa aaaagaacga agtcgagatc agggaatgag tttataaaat aaaaaaagca
    13441 cctgaaaagg tgtctttttt tgatggtttt gaacttgttc tttcttatct tgatatatat
    13501 agaaataacg tcatttttat tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg
    13561 tgttttaaag tattgaaaac ccttaaaatt ggttgcacag aaaaacccca tctgttaaag
    13621 ttataagtga ccaaacaaat aactaaatag atgggggttt cttttaatat tatgtgtcct
    13681 aatagtagca tttattcaga tgaaaaatca agggttttag tggacaagac aaaaagtgga
    13741 aaagtgagac catggagaga aaagaaaatc gctaatgttg attactttga acttctgcat
    13801 attcttgaat ttaaaaaggc tgaaagagta aaagattgtg ctgaaatatt agagtataaa
    13861 caaaatcgtg aaacaggcga aagaaagttg tatcgagtgt ggttttgtaa atccaggctt
    13921 tgtccaatgt gcaactggag gagagcaatg aaacatggca ttcagtcaca aaaggttgtt
    13981 gctgaagtta ttaaacaaaa gccaacagtt cgttggttgt ttctcacatt aacagttaaa
    14041 aatgtttatg atggcgaaga attaaataag agtttgtcag atatggctca aagatttcgc
    14101 cgaatgatgc aatataaaaa aattaataaa aatcttgttg gttttatgcg tgcaacggaa
    14161 gtgacaataa ataataaaga taattcttat aatcagcaca tgcatgtatt ggtatgtgtg
    14221 gaaccaactt attttaagaa tacagaaaac tacgtgaatc aaaaacaatg gattcaattt
    14281 tggaaaaagg caatgaaatt agactatgat ccaaatgtaa aagttcaaat gattcgaccg
    14341 aaaaataaat ataaatcgga tatacaatcg gcaattgacg aaactgcaaa atatcctgta
    14401 aaggatacgg attttatgac cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg
    14461 gaggaaggtt tacaccgtaa aaggttaatc tcctatggtg gtttgttaaa agaaatacat
    14521 aaaaa
//
LOCUS       DACCQP010000065        13979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCQP010000065 DACCQP010000000
VERSION     DACCQP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.067"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(269..1555)
                     /locus_tag="IVO87_002114"
     CDS             complement(269..1555)
                     /locus_tag="IVO87_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7450281.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1548..2816)
                     /locus_tag="IVO87_002115"
     CDS             complement(1548..2816)
                     /locus_tag="IVO87_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7450282.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3068..3529
                     /gene="mscL"
                     /locus_tag="IVO87_002116"
     CDS             3068..3529
                     /gene="mscL"
                     /locus_tag="IVO87_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7450283.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3588..4034)
                     /locus_tag="IVO87_002117"
     CDS             complement(3588..4034)
                     /locus_tag="IVO87_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450284.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4127..4372)
                     /locus_tag="IVO87_002118"
     CDS             complement(4127..4372)
                     /locus_tag="IVO87_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450285.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4487..5065)
                     /locus_tag="IVO87_002119"
     CDS             complement(4487..5065)
                     /locus_tag="IVO87_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7450286.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5142..5672)
                     /locus_tag="IVO87_002120"
     CDS             complement(5142..5672)
                     /locus_tag="IVO87_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7450287.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5676..6536)
                     /gene="rlmB"
                     /locus_tag="IVO87_002121"
     CDS             complement(5676..6536)
                     /gene="rlmB"
                     /locus_tag="IVO87_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7450288.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6529..6924)
                     /locus_tag="IVO87_002122"
     CDS             complement(6529..6924)
                     /locus_tag="IVO87_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7450289.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6921..8333)
                     /locus_tag="IVO87_002123"
     CDS             complement(6921..8333)
                     /locus_tag="IVO87_002123"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7450290.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8330..8866)
                     /gene="cysE"
                     /locus_tag="IVO87_002124"
     CDS             complement(8330..8866)
                     /gene="cysE"
                     /locus_tag="IVO87_002124"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7450291.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9188..10648)
                     /locus_tag="IVO87_002125"
     CDS             complement(9188..10648)
                     /locus_tag="IVO87_002125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7450292.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10818..11975)
                     /locus_tag="IVO87_002126"
     CDS             complement(10818..11975)
                     /locus_tag="IVO87_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7450293.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(12009..13379)
                     /gene="radA"
                     /locus_tag="IVO87_002127"
     CDS             complement(12009..13379)
                     /gene="radA"
                     /locus_tag="IVO87_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7450294.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13442..13969)
                     /locus_tag="IVO87_002128"
     CDS             complement(13442..13969)
                     /locus_tag="IVO87_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7450295.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3674 a         3051 c         2502 g         4752 t
ORIGIN      
        1 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
       61 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
      121 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
      181 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
      241 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
      301 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
      361 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
      421 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
      481 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
      541 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
      601 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
      661 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
      721 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
      781 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
      841 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
      901 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
      961 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
     1021 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
     1081 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
     1141 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
     1201 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
     1261 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
     1321 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
     1381 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
     1441 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
     1501 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
     1561 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
     1621 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
     1681 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
     1741 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
     1801 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
     1861 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
     1921 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
     1981 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
     2041 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
     2101 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
     2161 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
     2221 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
     2281 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
     2341 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
     2401 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
     2461 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
     2521 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
     2581 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
     2641 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
     2701 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
     2761 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
     2821 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
     2881 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
     2941 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
     3001 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
     3061 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
     3121 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
     3181 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
     3241 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
     3301 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
     3361 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
     3421 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
     3481 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
     3541 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
     3601 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
     3661 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
     3721 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
     3781 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
     3841 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
     3901 gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
     3961 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
     4021 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
     4081 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
     4141 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
     4201 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
     4261 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
     4321 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
     4381 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa
     4441 gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat
     4501 aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct
     4561 tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat
     4621 gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta
     4681 ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc
     4741 cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg
     4801 cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc
     4861 caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg
     4921 cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta
     4981 gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg
     5041 cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa
     5101 tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt
     5161 aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg agaattacgt
     5221 cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt
     5281 atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac
     5341 tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca
     5401 gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac
     5461 tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg
     5521 acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca
     5581 tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg
     5641 tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa
     5701 gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc
     5761 atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct
     5821 tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg
     5881 gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc
     5941 aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg
     6001 ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg
     6061 tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa
     6121 aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat
     6181 tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct
     6241 aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt
     6301 tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt
     6361 tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca
     6421 ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt
     6481 tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt
     6541 ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt
     6601 caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc
     6661 agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc
     6721 ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc
     6781 agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat
     6841 caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc
     6901 gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct
     6961 tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca
     7021 cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac
     7081 tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct
     7141 tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca
     7201 agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg
     7261 aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct
     7321 gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg
     7381 gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc
     7441 gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg
     7501 aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg
     7561 tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt
     7621 tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa
     7681 tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt
     7741 gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct
     7801 tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg
     7861 gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct
     7921 gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc
     7981 atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct
     8041 tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt
     8101 gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag
     8161 cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg
     8221 atatggatat aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt
     8281 atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc
     8341 tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt
     8401 cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg
     8461 ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt
     8521 gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat
     8581 cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc
     8641 cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag
     8701 cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc
     8761 aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg
     8821 gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct
     8881 ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat
     8941 aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt
     9001 atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt
     9061 tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt
     9121 gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc
     9181 atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt
     9241 cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca
     9301 tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga
     9361 cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag
     9421 taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat
     9481 tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca
     9541 ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc
     9601 catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga
     9661 tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta
     9721 ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca
     9781 caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt
     9841 cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg
     9901 tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt
     9961 catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag
    10021 tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc
    10081 gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca
    10141 ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta
    10201 gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt
    10261 aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg
    10321 tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga
    10381 tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat
    10441 cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat
    10501 tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa
    10561 acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg
    10621 gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac
    10681 taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag
    10741 cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag
    10801 ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg
    10861 tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga
    10921 agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg
    10981 acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt
    11041 actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac
    11101 gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca
    11161 ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca
    11221 ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag
    11281 gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc
    11341 gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat
    11401 ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta
    11461 tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct
    11521 gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt
    11581 gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca
    11641 ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc
    11701 aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt
    11761 ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa
    11821 aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac
    11881 tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca
    11941 atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca
    12001 tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc
    12061 tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg
    12121 aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct
    12181 ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag
    12241 cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca
    12301 cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc
    12361 actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt
    12421 ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc
    12481 attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt
    12541 gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc
    12601 gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct
    12661 tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct
    12721 tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt
    12781 agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc
    12841 tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca
    12901 cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg
    12961 aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact
    13021 tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata
    13081 cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg
    13141 ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt
    13201 tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct
    13261 tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac
    13321 ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt
    13381 gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt
    13441 tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg
    13501 cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat
    13561 ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt
    13621 aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat
    13681 tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag
    13741 taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat
    13801 ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct
    13861 cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga
    13921 tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc ggttttcct
//
LOCUS       DACCQP010000066        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCQP010000066 DACCQP010000000
VERSION     DACCQP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.068"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IVO87_002129"
     CDS             131..505
                     /locus_tag="IVO87_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7450296.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVO87_002130"
     CDS             complement(619..1248)
                     /locus_tag="IVO87_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7450297.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVO87_002131"
     tRNA            complement(1382..1454)
                     /locus_tag="IVO87_002131"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVO87_002132"
     tRNA            1668..1741
                     /locus_tag="IVO87_002132"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVO87_002133"
     CDS             1696..1845
                     /locus_tag="IVO87_002133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450298.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IVO87_002134"
     CDS             2338..3666
                     /locus_tag="IVO87_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7450299.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IVO87_002135"
     CDS             3868..4701
                     /locus_tag="IVO87_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7450300.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IVO87_002136"
     CDS             4717..5454
                     /locus_tag="IVO87_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7450301.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IVO87_002137"
     CDS             complement(5609..6577)
                     /locus_tag="IVO87_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7450302.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IVO87_002138"
     CDS             6809..7642
                     /locus_tag="IVO87_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7450303.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IVO87_002139"
     CDS             7676..8515
                     /locus_tag="IVO87_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7450304.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IVO87_002140"
     CDS             8541..9101
                     /locus_tag="IVO87_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7450305.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IVO87_002141"
     CDS             9300..11021
                     /locus_tag="IVO87_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7450306.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IVO87_002142"
     CDS             11186..12328
                     /locus_tag="IVO87_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7450307.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IVO87_002143"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IVO87_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7450308.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACCQP010000067        13831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCQP010000067 DACCQP010000000
VERSION     DACCQP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.069"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            149..1552
                     /locus_tag="IVO87_002144"
     CDS             149..1552
                     /locus_tag="IVO87_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7450309.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1901..2533
                     /locus_tag="IVO87_002145"
     CDS             1901..2533
                     /locus_tag="IVO87_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7450310.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3121..4560
                     /locus_tag="IVO87_002146"
     CDS             3121..4560
                     /locus_tag="IVO87_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7450311.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4855..5073
                     /locus_tag="IVO87_002147"
                     /pseudo
     CDS             4855..5073
                     /locus_tag="IVO87_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5322..5537
                     /locus_tag="IVO87_002148"
     CDS             5322..5537
                     /locus_tag="IVO87_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450312.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5754..6010)
                     /locus_tag="IVO87_002149"
                     /pseudo
     CDS             complement(5754..6010)
                     /locus_tag="IVO87_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6197..7108)
                     /locus_tag="IVO87_002150"
     CDS             complement(6197..7108)
                     /locus_tag="IVO87_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450313.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7127..8131)
                     /locus_tag="IVO87_002151"
     CDS             complement(7127..8131)
                     /locus_tag="IVO87_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7450314.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8128..10251)
                     /locus_tag="IVO87_002152"
     CDS             complement(8128..10251)
                     /locus_tag="IVO87_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7450315.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10256..12703)
                     /locus_tag="IVO87_002153"
     CDS             complement(10256..12703)
                     /locus_tag="IVO87_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450316.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12690..13079)
                     /locus_tag="IVO87_002154"
     CDS             complement(12690..13079)
                     /locus_tag="IVO87_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450317.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13139..13642)
                     /locus_tag="IVO87_002155"
     CDS             complement(13139..13642)
                     /locus_tag="IVO87_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7450318.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13655..>13831)
                     /locus_tag="IVO87_002156"
     CDS             complement(13655..>13831)
                     /locus_tag="IVO87_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450319.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4277 a         2714 c         2519 g         4321 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      481 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      541 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      601 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      661 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      721 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      781 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      841 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      901 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      961 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
     1021 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
     1081 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
     1141 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1201 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1261 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1321 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1381 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1441 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1501 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1561 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1621 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1681 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1741 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1801 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1861 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1921 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1981 ctgtgggaat gaaacgattg tatatcgatc aaacttacta acaggtagaa ccaaaagttg
     2041 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     2101 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     2161 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2221 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2281 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2341 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2401 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2461 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2521 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2581 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2641 tttgattgac aaatatcgag gtaatcatta attattgtgg ataataatta acagtgatat
     2701 ataaacatct ataatatatt attcatacta ttgatattta gtataatcct atggaattga
     2761 atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat
     2821 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
     2881 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
     2941 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
     3001 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
     3061 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
     3121 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
     3181 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
     3241 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
     3301 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
     3361 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
     3421 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
     3481 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
     3541 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
     3601 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
     3661 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
     3721 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
     3781 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     3841 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     3901 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     3961 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     4021 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     4081 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     4141 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     4201 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     4261 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     4321 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     4381 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     4441 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     4501 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     4561 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     4621 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     4681 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     4741 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     4801 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     4861 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     4921 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     4981 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     5041 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     5101 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     5161 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     5221 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     5281 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     5341 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     5401 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     5461 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     5521 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     5581 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     5641 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     5701 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     5761 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     5821 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     5881 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     5941 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     6001 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     6061 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     6121 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     6181 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     6241 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     6301 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     6361 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     6421 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     6481 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     6541 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     6601 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     6661 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     6721 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     6781 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     6841 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     6901 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     6961 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     7021 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     7081 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     7141 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     7201 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     7261 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     7321 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     7381 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     7441 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     7501 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     7561 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     7621 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     7681 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     7741 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     7801 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     7861 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     7921 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     7981 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     8041 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     8101 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     8161 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     8221 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     8281 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     8341 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     8401 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     8461 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     8521 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     8581 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     8641 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     8701 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     8761 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     8821 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     8881 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     8941 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     9001 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     9061 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     9121 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     9181 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     9241 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     9301 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     9361 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     9421 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     9481 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     9541 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     9601 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     9661 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     9721 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     9781 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     9841 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     9901 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     9961 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
    10021 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
    10081 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
    10141 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
    10201 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
    10261 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
    10321 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
    10381 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
    10441 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
    10501 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
    10561 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
    10621 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
    10681 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
    10741 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
    10801 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
    10861 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
    10921 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
    10981 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
    11041 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
    11101 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
    11161 ccgcaatttc tggcaaggtt tctttccagc caccccgttc cccttttgga tcgacaatca
    11221 cagcttgtcc accaaaaagg acggcgtaat aaactaagag attgttacta aaggactttc
    11281 cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt ggtaactgaa tccttgaccc
    11341 cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc cgtatctacg ttataaccaa
    11401 agtaaatgcc ttccaactcc cctaacatct gagtggcgcc aaaaccaagt ccggcgagaa
    11461 aatcagacgt aacatactgg atataatcat tcatgtatcg cttactagaa ggtaagaatt
    11521 cttcatgtag ccccatcata tcgccaaaag gccggactag ttttacattg gtatcatcat
    11581 aaaaatctag cacttcatca caccggcgct ttagttcatc tactgattcg gcacttactc
    11641 gaaccacata gctcagtttg tacatggatt ctttggactg atccaaggta gtttccaatt
    11701 catctacgga atccaatgca tctaagacat tggaatttgt ttcattgtca gattgatagg
    11761 catggttgtc caagtctttt aattcctttt tcttattgcg aacggtagca agtgcctttt
    11821 tgttcgtcac aatctccaca ttcatactag tgtcaattgg gaatgtaaat tgttgttgct
    11881 ggtaatagaa aagttctgag gagggaaact ccatttcccc cacaattgta ttaattgtta
    11941 agtacgctac atatgattca tgattctcat gttccatgca taagtaccgg ggcttttctt
    12001 caatcaaaca acgactggga cgtaataagt cgtaacgttt caccaaagtt tccgacttca
    12061 gctttttctt tggtagctga aattcatatt cttcaaatgg tgtacccttt tcgccataga
    12121 tatgttcaat caaatacatc agatcactcg gtgtgactcg tctgacttta aatcttcgag
    12181 ccaatttact ttccattagt ttttcaagct tggtatatcg gcgaatttct tcattggata
    12241 aggaaacaaa atcgcccatc aaatgatgat tcactccata gacaaattct tgaaagccgg
    12301 agaagaaaga tttcttgaga ttctttagat tgacctcttc atctgtcgca atcagtttga
    12361 agccaataaa aaagcgataa tcaatctgac tatcgccaat catggatact aatgcctctg
    12421 tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg cccggtaatt tccttttttg
    12481 atcgttcttg cgtggcacga atactacttt ctgtggcaat ttgtaaggca tgaatcttgc
    12541 cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg gacttgatat ttctgttcag
    12601 gtgacaaaaa ggagtaattg taaggtatta actcatagta ggcaaaacat tctccctctt
    12661 gattaaagac aagattattt tcaatatact taatcgggta catagttcac actccttacc
    12721 agagtaatgg gttcgtccca tcgtttcttc tgttctttga ctttctttcc tgcgaaagtg
    12781 accttaaatc gtaagtgata gctaataaaa gatcgtaaaa atcccaatgg tttcttaccg
    12841 tcaaacgttt tttgcgacat gaaccaagta actgccaccg gtatacctaa gtattttaaa
    12901 aaggccccct ctatcataga tagtggaggc acatgggcaa atacgatgac aaacagcaag
    12961 gataaaacaa accaagccat ctggttaaaa gtcacaggaa aaggcagttg aaagtcatta
    13021 attgcataaa tgaccttttc aactgcccaa atactggtgt agctttttat tttcttcatc
    13081 aaatcattcc tttaaaaaga aggatagttt agaaatcaac tacccttcaa taattttgtt
    13141 aatatggata ctcaaaaaca cctcgactcg ttaccaaata attgcctcca atctccaagt
    13201 ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg ttctggtact tctccaaaat
    13261 caccagcttc tacaatcaag aaatatgcta catcactcat atcatcgcaa tctggataat
    13321 agataatatc atccacatgc tctaccatct cctcaaaaga attgaagaaa gcatgttgaa
    13381 tctcagaagc aacttcacca actggcgttc cttcaagttc ctccgctaag ccacacaacc
    13441 gattaatttc ctcaattggc gtatactcat caatctcaaa gggaagttca taatcatgaa
    13501 ttgcatattc ctcgtattcg tcatttaacc caattcgttc tttcacttca tcaaaatcga
    13561 caggtggggt aaaccacgcc cccaccaatt caccttcgtt atatttcccg aggttcgcaa
    13621 tataaacacg catctgttcc atcgtgcttc ccccttacgc accgacaatt cggttaaaca
    13681 aactgagtaa gacatccttc accccagaag cattaaagac caatccgacc gcaatcaaag
    13741 caatcaccaa aaagccaatc agtttggaaa actcccgctt aaatcctaaa aacaaaccaa
    13801 tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DACCQP010000068        13826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCQP010000068 DACCQP010000000
VERSION     DACCQP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.070"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(22..1041)
                     /locus_tag="IVO87_002157"
     CDS             complement(22..1041)
                     /locus_tag="IVO87_002157"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7450320.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1674..2678)
                     /locus_tag="IVO87_002158"
     CDS             complement(1674..2678)
                     /locus_tag="IVO87_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7450321.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2691..3740)
                     /locus_tag="IVO87_002159"
     CDS             complement(2691..3740)
                     /locus_tag="IVO87_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7450322.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3757..4578)
                     /locus_tag="IVO87_002160"
     CDS             complement(3757..4578)
                     /locus_tag="IVO87_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7450323.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4565..5344)
                     /locus_tag="IVO87_002161"
     CDS             complement(4565..5344)
                     /locus_tag="IVO87_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7450324.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5361..5831)
                     /locus_tag="IVO87_002162"
     CDS             complement(5361..5831)
                     /locus_tag="IVO87_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7450325.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5843..6262)
                     /locus_tag="IVO87_002163"
     CDS             complement(5843..6262)
                     /locus_tag="IVO87_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7450326.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6343..9111)
                     /locus_tag="IVO87_002164"
     CDS             complement(6343..9111)
                     /locus_tag="IVO87_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7450327.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            9311..9535
                     /locus_tag="IVO87_002165"
     CDS             9311..9535
                     /locus_tag="IVO87_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450328.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10314..12029
                     /locus_tag="IVO87_002166"
     CDS             10314..12029
                     /locus_tag="IVO87_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450329.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12032..13795
                     /locus_tag="IVO87_002167"
     CDS             12032..13795
                     /locus_tag="IVO87_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450330.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4064 a         2829 c         2401 g         4532 t
ORIGIN      
        1 gattaactat tgatcatcta tttaacgaag agaaaattca ttctccattt ttccgtcacg
       61 taacatgatt tcttttgctg cttttttgat atcttctcca ttatacaaaa cattatagat
      121 tgtttgagta attggcatct caacacctag ctgttgagct agctctactg ctgcttttgt
      181 cgttgccaca ccctcaacga ccattcccat gttttctaga acttcttcca gcttatgtcc
      241 ctgtcctaac aagttaccgg cacgccaatt gcgagaatga acacttgtac aagtaacgat
      301 caagtccccc acaccactca aaccgataaa tgtcagagga ttggcaccca tcgcgacacc
      361 aagacggctg atttctgcca atcctcgagt catgattgct gcttttgcat catctccgaa
      421 tcctaatcca tggatagcgc cggcaccaat tgcaatgata tttttcagtg ctgcaccagt
      481 ttctacgcca atcacatcat tatttgtata aatcctgaaa taatcattca tgaacagttt
      541 ttgtacatag gctgctgctt tttgatcaat acttgcagct gtaattgttg tgatatcatg
      601 tactgccact tcctcagcat ggctaggacc agataataca acaattgcct gtcttttttc
      661 ttctggaatt tcttcagcca aaacttctga gattcgttta tgcgtatctt gttctagtcc
      721 tttacttgca tgtataatga ttggcttagt attcatttta ccaataagtt cctgtgcgac
      781 tgaacgaatc gcttttgttg gaacaacaaa taaaatagca tctgcgttat ccactgcttc
      841 tgccaattct ttatatccga taatcgattc tgggatcgtc aaatcaggta aaaaatgctg
      901 atttgtatga tacttattga tttcatcaat ctgttcaggt acatttcccc aaatacggac
      961 atcatgcccg ttttctgcca atgtttgtgc taaagcagtg ccccaagatc caggaccaag
     1021 aactgcgatt ttttgtttca tggatattcc tccaaataaa taattgataa tttccattat
     1081 agtatacatt tcttaaagtt gggactaatt tccattgttt ttaagtgttt gtttacttct
     1141 tttatcgttt ttacttgaaa taatcaatga tatgttacaa tagtctacat tttattttta
     1201 cactcgttat gctttgcttg ttcctaagag gaagcagttt tgacaatgtt actaccagca
     1261 ttactattag gaggttagga aacttctcct agtgagtatt gatttaaaat acataagaga
     1321 cagattgaac atttctaaat ctgtcaggtc ctgcaacgga cagattgtct ctgttgacgg
     1381 ttgacatagg aacttttgtt ttagtcaaca ttctggatac aaaacgctat gtaaactggc
     1441 gcaactcgtt gtacttggac ttttactacg gatccatgca acaactaaat agtactattg
     1501 cataacaaaa aatggttggc acccacatgt cccattaaac agattataag ttgaactgta
     1561 gataatgcaa aaaatgtgtt ttataacgta attgatgcca atattttaaa aaaataaagg
     1621 gagcagttat cgctgctctc tttatttttt atattctttt tatccagtct atcctaataa
     1681 tcaaactgac gatagtagcg gcgtatctct aatggatggc agttcaactg ctccacatga
     1741 gcatcgatgc gttgtgtcac tacatgcatc aataagaaag acaaaacacg tccgcgatat
     1801 ttttcactga taccttctac tggatagtct tttgtgtcta tcaatgtata attgctgcaa
     1861 atttttggca aaagtttagc cacacgttca gataacactc tctgttcatc ttcgcctaaa
     1921 aataatgtga ctggggttgt ttcttctacg atctctaacg tcccgtgaag aaactcggca
     1981 gcatggatag atttcgatgg aagccagctt tgttcttccc agtaacacat ggcatatgaa
     2041 taaacagctc cccattgatt ccctgctcca ataaaataat gcatactatc acgtcgatgt
     2101 ttttctgcaa atgataagcc aaatgcatct gcttttttct ctgcttccac taaccctgtt
     2161 ggtaaataac gttcgagctg ttcatagtat tctgaataag cttcgaattc tccattcaaa
     2221 tacatcaatc gatctgctgc cataaaaaat ttgatttgtt ctgttccagg agctggataa
     2281 gtgaccacaa aatcgcatag ctgagacaac ttactgttcg ctttatcgat aaatccaatc
     2341 agtgtcgctc ctactttctt gatttgttct actgctttca ctacttcttg agttgtaccg
     2401 gtaacagagg acaaaataac gatagaatct tttgttaatc ttttgtttcc agtagtgtaa
     2461 tattctgcag catgctgtac aaagacaggc aagttgcttt tcccattcat ataagtgaca
     2521 gcttgcatcg aggaagcata cgttccaccg attcctagat aatagacagc gtcaaatttt
     2581 tccgcataaa gtttatcgat gatttcttct acttgcggac gtaaagctag tgcgcctttt
     2641 attgctttga tttgttcttc ttcattgaat ttcagcatga tttccacctc ttataattta
     2701 cttttcaaga ctgtatcaaa gtcttcaaaa atctttttgt tcaagtcaat acctttagct
     2761 actgcggttt tataagcgaa tagttgaaac ggaacgacaa gcgctaatac agacagatat
     2821 tcatcacatt ctattgctaa tccaagtgtt ttctcgtttc ttgctttttt tgtggtaatg
     2881 gtgagagtct ctccaacgta ccgactcata tatctttgca acttttgcga acgttcctta
     2941 tttacacttt ccccttcaat aaagaatagc aaatgcgaag catctgcctc caggtatggg
     3001 ccgtgcatat aagcttctaa ctcaaatcct tgtgacggaa cgcggattgt ttcagtcaat
     3061 ttcgtctctg cttctttagc tgttccccag tttggaccat atccaatggt gacgaaacgc
     3121 tgtgctaaac gaaataaact ttgatatcca tcaaaaaatt cttccgtttt ttgaataatc
     3181 gctggtaaat ggttgattat tttgcgcaac tgttgcatgt agtcttgttc tatttttttg
     3241 ctgattttat ttttacttat tccaattcct atccctaaga gtagtaactg taaaacagtc
     3301 gcagaatagc cttttgtaac gaagcctact gattctaccc cggcacctaa atcaataatt
     3361 tgtgcagctt ctaaagcaag tggactttgc acgtcatttg tgatagcaac aacaggtaac
     3421 ccttgttttt gtatcatttt tactgcttca acagtagaag cacttttccc gctttgtgac
     3481 acagcaatca cagtatcgat tgaaggagaa agttttccat aattgaaaaa ataaaaaggt
     3541 tcttgaatat cgacagtaag atcaccataa gattccaatg ctggcttcgc tgctaaacaa
     3601 gcgttataag atgaacccgt tgctaagatc agtaaattca ctgttttttt catatgttct
     3661 cttgtcgaaa aatcattctt tttcaagatc gtacttagta cttccttttc ttcatagata
     3721 taatcttgca tcgtaaacat tttctctgct ccttttttaa aaaatcccta tccacgaacc
     3781 aaaaattcca aagaaagcta gtccgatcaa gatcgttaat ggtttcactt ctttcttcaa
     3841 tagataaaac actatgccaa atgctccaag acataagata tttggaacga tatcatcaaa
     3901 aatactttga acagtcacag cattttctcc tgaaccgatt tttaaaggaa tattgatggt
     3961 gatcatcgtg gcagtcattg ctccaacgac catcagtcca ataatagacg cgcctaatgt
     4021 caaactttcc atcataccgt ttgcctggat ctttttcaaa aaacccgttc ctaagcgata
     4081 tccccaacct gttgctagat agcgaaacag aatgtgcgga atattgaaaa tcaaaatgaa
     4141 caagataggg cctaaaatat ttccttgcat ggctaaagag gtacccacac ctgtggcaat
     4201 caatcgcaaa gttccccaaa agaaagaatc tcctaaccct gcaagaggtc ccatcaaaga
     4261 tgttttgata ctatcgatcg cagtcacatc aaattcagga tcatccttat tttcttcctc
     4321 cattgcagcc gtgattccta aaatcagtgt aacgatatgt ggggtcgtgt taaaaaactc
     4381 tagatgccgt ttcaaagctg ctgccatttc ttctttcgac tgatataact ttttcaaaat
     4441 cggaatcatt gcataaacat aagccatatt catctgacgt tcataattcc aagaaaactc
     4501 catttgaaaa gaacgccaaa atacgtgatt tagctctttt ttcgttattt tgctgttttt
     4561 gatctcagaa ttcttcatct tcttctacct cccctgcagt tgtttgaagt ggtagttgac
     4621 ggacattcat aatattgact accacaactg ctaaaatcgc accaaaaata gctactccgg
     4681 tcacaggaat ctctaagtaa gaagctatag caaaacctaa gaaaaaatat ggtgcaactt
     4741 gtttgttgat cagcaaacga gcaagcatcg caaaaccaag agctggaatc aaccctgtag
     4801 ctactgacaa ccccttttga acgaagtcag gaatcatatt taatagattt ccgatcgtat
     4861 tgcttcccac catataagaa accattacca caacaccaag catcaaggaa agaccgatac
     4921 cggcaagcaa gtgcatatgc tcgactcctt tataatttcc ttcttctgca taattgtctg
     4981 ctttatggtt cataattgga atcaaaagcc ctaagtaaat atttttcaat atcaatgtca
     5041 acgtagcaat cggcaaacct agtaaaagcg cggtttctgt accggctcca gctgtaatag
     5101 caaaagccgt ccctaaaatc ccaccggtca caacatcagg tggaatcgaa gcacctactg
     5161 agaacgatcc gataaatgct aactctaaag ttgcgcccat aatgatccca gtttttacat
     5221 cacctaacac gattcctgta aacagccccg tcacaatcgg acgagaaagc aacgatgtcc
     5281 ctagtgcata ttctgattgt gcgacaaatg caacaattcc taataagact gcttgtacaa
     5341 tcattgaggt tcttcctttc ttacaaaact tctcttacat ccactttttt atctgctggt
     5401 acttgacgga tttctacttc tatttgtttg tcaattaatc gtttcaagcg ttcctcctct
     5461 tctggtaaaa tatttaccgc ttttccgatg ctgcgagtac caggttttgc cttgatccct
     5521 cctaagttga tggttttgat ttctggatac gcttctgcca gcttatatgc atcctcgatc
     5581 gattccacta cgatgaataa cttatattta tctgtcaccc cgcttttcaa cgcggcaatc
     5641 gagtcatcaa ttgatttgat cactagtttg actccttgtg gcttggctaa tttgatcgtt
     5701 gtctttcgta tctcattcgt aggtacatca tcattagcaa tcaaaataca gtctgcgcct
     5761 attgtttgtg tccatgaaaa agctacttgt ccgtgtaata accgatggtc gactcttgtc
     5821 aatacaatca tttttcttcc tcctaaaatt cgtcttcttc tattttctga ttgagccgct
     5881 cgttacaaaa ctggatcatt ttttttgaat cagttagcac ctgctggatc aaaacagttg
     5941 tctctacttc agcgtccaac cgagatacta gctcaatcaa tagtggcaaa ttcaatccgg
     6001 caacgagata gacatgatcc cgattgatat attgtaagaa ttcgttgttg acacttccgc
     6061 caaaaagatc cgtcagaaca atgatttctt tatctttgta cctcataaaa agctgcctta
     6121 ctgtttccgc taaatcaaaa ttctcatcag tatagcaatc gaatacagta atagaatgat
     6181 ttttcccata gatcagctct aatgaatcta atatccctga agcaaatttt ccatgggaag
     6241 ctaagataat ttctctatcc actttttcat ctcctctttt cacttacaca tactaaaaaa
     6301 gcaagttgca tgccaaaatt agtggcaaca acttgcttat gttcaaaatt cttcttctat
     6361 cgagagaaac tctgtatctt cactcaaaat atcatagata taggcaatct cagaattggg
     6421 gatcatgaca ctataatgct cttcaatgac actaaacgca gatttaatag cagataaccc
     6481 ttctttttga cactgcttcc atttctctaa tccttgatag gtttcgattg gcatttgtcg
     6541 gatcaatcgt tcaatgagac agctcacatg gacatacaac gctaattttt tgggattgct
     6601 gatgcttcga ttcaactgca cctctaattc tctcataaaa tgatcgattt ctcccatcac
     6661 cttctctgta tctagaatag tcacggaatc cagtactttt tccaatgaaa aattacgaat
     6721 gatgcgctga ttgaatattt ccttctctga agcattcata gtagtgtcca accattcagc
     6781 tagtacagac atcttttccc ccgagatgag gtcttctaat gaaagatagt cgatattggt
     6841 gatatcagga tctgtggtgc caatcattcc tacgacttcg tatatggaaa ataatgggtc
     6901 actgtttttc ttatctttta attgctgata ttcatatggg atgattttca actgacaagc
     6961 cgtaggtaag cttttttcta gcagttcact taagtggacc gccgtaccga ttccagtgaa
     7021 gcaagtagta atgagtgctt tgggtttgtt ttcctctgga tagagcagtc gccattctgg
     7081 ctggatctgt cgaacagtct cttctgctat ctcttctaat gatacatttt tttgcaggca
     7141 ttcccctact gtgattgcta aaggtgtcgt cacattattc aaaatcaaaa gcggtacgga
     7201 taactgtttt ttaaaaaact gatggatttc tttcagagat cccatgtcta ctaatatgat
     7261 caatccattt gaaacatcgc tgttttcgca ataatcaagg atttctgaag caatctgctg
     7321 tggtgtgaca tctagtggca tatcaaatga ttcaaataga tcctcttgta ataaacgatt
     7381 tactacattt gccatactgc tagcagttgc ataaccgtga gcagcaatca tcgcttttgg
     7441 cagtcgtttt tcttttgacc atttcgcttt ttttagatag atcgtcagta aaatccgatc
     7501 catacttgtg tcttcaatat ctaatttgat cttacttaat tccagaattc gttggacata
     7561 gtgataacta gatggataag actgagaaac ttgttgttct aattctttga ttagttggat
     7621 aatatcatgt tcttccggta accatttgat agtgcttcgc tgatagagat agtaactcaa
     7681 ggcatagaca ctgtttccat cgaatttgat ttgataggca gtttccattt gtcgaaaggt
     7741 ctctcggata gattgtgtcc aataaaccag cagctcgtgt ttttcttgtc tactggtctc
     7801 aaacagcaaa aaatcaaata attgaatcac ttcttgcttt aattttcctt cgcaagaata
     7861 tagcagacag ttatttttct tgaaatcaat taggatttgc tcaaaacttt taacgattcg
     7921 ctgctgttcg ggctgttgct tctccattaa ttgctctaag gtctggtctt gcgtaatata
     7981 gattccttgc tgtgctgtat ttttagtaat aggctgcgca agtagttctt tttgcaaatg
     8041 atggatcgtg acatggattt caggctgttc acgctgttca gcatatgctt ttgctgctga
     8101 aactttgacg atatttttca gctctcctat gtttccggat aatttctgac ttgttaaaag
     8161 tgacaaagct ggtccactga ctgacaaagg gctccccatt tttctttgtt cttcgagaaa
     8221 aaaagagtaa atcagttcta gccgttcatt acgactacgc tgattaagtg caggtagttc
     8281 gattcggata ggtattcttc gaatgaaagt tgttaagaaa tgactagtaa tctcttcagt
     8341 cgtagcaaaa aagagacgtg ttttgatccg gcgtgacaaa ttaggttctc ccatacgata
     8401 aatcacacct tgatcaagat aggtgaacag tttttcctga ccttctggtg ttaagcgatg
     8461 tacctcgtct aaaaacaaga tcccaccatc tgctgcttca aaagcccctt ttttattttc
     8521 ttctgcacca gtgtacgctc ccttaacatg tccaaacaaa ttgctagtca aaagttcagg
     8581 attatctgca tattgtgcac aattaaaagt gatgaatggt gcagaatctt ctagcagatc
     8641 atgaagcaga caatactgat agaccaaatt aactaaataa cttttacctg tgccactttc
     8701 gcctgtaatc aaaagtggta atccgccatc tggatagtaa agagctgttt tgatttgttc
     8761 gatactttct ttcaaactgg cgtcgtatcc gatcataaaa gagaataagt cttttggttg
     8821 cgcgaaaaaa ggctgttcct ctttgagatc atcgatagaa aaataaaaat tcgtgcgtaa
     8881 cttaaaaaat tgtttttcaa aagcttcttt gtggaaataa atcactggtc gagaattgat
     8941 tttgactagt agttgctcat catttaactg gttcaaataa tgactgactg tgtttctttt
     9001 taccccaaaa tgatcagcta acctttgtgc tgtaaagacc tcgttcatat gttctaaatc
     9061 gaaaaaggct gtctggttct ctaagtaaca taaaatttcc ttcttcaaca acttattcac
     9121 ccctcctcca tcatagaaaa cggattcatt ttgtcaataa aatctttaat aaagaattga
     9181 aaaatatatc ttattataga cagcagccta cttatccctt ataattttta ttctaaaaaa
     9241 cgtacagttc cttccagttt ttatactgga atttcaacat atgaaagaat aaggaggcta
     9301 tgactattca atgaaatcgg ataaatataa aaacaggaaa catacttcct ggaaaacttt
     9361 tcgagaaatc atcggatctt atctcgctgt agttgtattt ttgcctttgc tttatatggc
     9421 tatgacagaa caaactcgtt ttattggcgt agatacgcaa tatatagccg aaacaggatt
     9481 tttattcatc tttatcttga tttttgcgtt ctataaatcc ttccatacaa aataagattc
     9541 acagatttca actttcctgt ctgtttttag ctattacaaa tccttatatg tggtataaca
     9601 acaatttatt ggcataatga ggaaaaacga gttatgatgc tttcagagag taaagaggta
     9661 ctgttgtcac catccttttt attgattacg tacaccactc ttagtttttt cccattgatt
     9721 acgaaaacaa ggtagaaaca ttgatgttcc taccttgttt tcttttttta ttttcagatc
     9781 aaaatctcaa gtagcttttg acttgatgtc gggaggttgt cttcgttcca tcaaacctga
     9841 tagaatctgc gccaaaccat agttttctcc actattattt attcctctaa gatagtaccc
     9901 acaataaacg aataaatatt tttctcattt tcatactcct tctttgaaaa tcaattagat
     9961 ttctctcaac ccactttttt acaaaaatta cgtagcaaaa aagattgcaa gaacaaatta
    10021 aaaaagaagt aaatagagtt tcatctataa acatttactt gatatatttt ttatgcagaa
    10081 ttcatttata gaaaaattaa gtaaaataga cgttcctatg aatgaagaga ctggcatgtt
    10141 ttctcatttt ctttttttta aaaaaaatag tctattcaat ttctaaagct tcttcataat
    10201 aaaactttca tttatgaaaa ggcttgttct cttttcattc taaatttcct aagctacaat
    10261 gtggtagtac ttgttttttc aattgtggac aaataaataa taggaggaat catatgattc
    10321 agttattacg ttatgcaaaa gactatcgta agcaaatcat tttgggcccg ttcttcaaat
    10381 ttcttgaagc tgtcttcgaa cttgtcctgc ctttaatgat ggcttccttg atcgataatg
    10441 gactaaaaat gaatgaccga ggaaaaatca ttgaaatggg cttatggatg gtcgctatgt
    10501 ctgtgattgg tctgatttgt gctatcattt gccaatacta tgcttcagta gcttcacaag
    10561 gatttggtac agagttacgt aatcagctaa tcaaaaaaat caatactttc tctcacaacg
    10621 agttgaatca ttttggaaca gataccttga tcacccgaat gacaaacgac atcaatcagc
    10681 ttcagctagc tttagcaatg gtcattcgtt tattgatcag agcaccgttt ttaagtatcg
    10741 ggtcagtggt catggctttt gtgatcgact gggaagtagg tctgtttttc ttagcattat
    10801 tgcctatttt ctcaatcatt ttatttttta tcatcaaaaa aaccgttcct ctctaccaaa
    10861 aagtgcaaga aaaactagat cagctcaatg aacacgttag tcagaatctt agcggtgttc
    10921 gtgtcatccg cgcatttgcg cagacaaaaa aagaaacaga gaagttcgat aaaagcacaa
    10981 atgatctagc gaacaattat ttacgtgttt ccaatatctc tgccctatta tcacctgcta
    11041 caaccttgat catgaatgtc gggatcatct gtttgctgac agttggcggg atcaaagtaa
    11101 atattggtag cttgcaacaa ggacaagttc ttgctttgat caattatatg aatcaaatgc
    11161 ttttggcact gatcgttgtt tcaaacctcg tcgtgatctt cacacgtgcc gaagcttcag
    11221 gaaatcgggt gaaagaggtg ctagacacag aaaattcgat tcttgaagct gaggtcagta
    11281 ctactcccga tttttcttct gaagcaatta tcgcttttga ccatgttgac ttccgttata
    11341 ctcctgattc tggtctttct ctccaaaata ttacatttca gctgaagcga aatacagtcc
    11401 ttggaatcat tggtccaacc ggaagcggga aaacgacttt gacacaattg attccgcgtt
    11461 tctatgatgt cagttccggt acagttataa tggacggaat ggatgttcgt tcttggcagc
    11521 tggatcatct ccgtcagcaa attgctcttg ttccccaaac ttctgtacta tttaccggga
    11581 ctattcgtga aaaccttcaa tggggtaaga aagacgccac agaaaaagaa tgttgggaag
    11641 ctctgaaaat cgctcaggca gaagaatttg tccgtcagct tccaaatggt ttagatacaa
    11701 aagtcatgga aaatgggaaa aatttctctg gcggacaaaa acagcgactg acgattgcaa
    11761 gagctttgat tgccaagcca gcattactga ttttagatga ctctttaagt gcgttggatt
    11821 atcaaacaga cttggatttg cgtaaggcgc tacagactta tcttggcagt acagtgatta
    11881 ttatctccca acgtgtccgc tctattcaag aagcacatca tatcttagtg atggatcaag
    11941 gtaagattgc tgctcagggt acccatgaag aattacttgc tcaatcagca gaatatcagg
    12001 aaatcgtcgc atcacaggag gaagaatagt tatgaaagta aaaaaattat ccttgcagac
    12061 gctccgccga ttcattcctt accttactgc ctatgcaaaa gacatttggc tagctgtgat
    12121 actaggaatc atcaatggga ttgcaacagt tgtgatgacg ttacaaattg gtcaaagtat
    12181 cgatcaaatg attggagaag gacaggtccg cttcaatcga ctgttccatt tacttattct
    12241 attcggtggt atcgttttaa tcaacgtagt cagtcaatgg ctcatccaag tgttaagcaa
    12301 tcggttagct tacttatccg ttgcaaaatt aagaaaagat gcatttgctc atctgaatcg
    12361 attgccactt cgttattatg atcaaaactc ccacggaaat atcgtcagtc gatttacaaa
    12421 tgatatggac aatatttcca ttgcaatttc tgcggccttc aatcaattat tttctggagt
    12481 ggcaatcatc attctttcgt ttatttttat gatgcgtcta agtccacttc tgacattggt
    12541 cgttctttgt tctacaccaa ttattttcct tgtgagttgg atcgttgctc gtgcttcaca
    12601 aacaaatttc gatagacagc aaaggattat tggggatatc tcgggctttg tgaccgaacg
    12661 aatcggtaac cagaaaatcg tgaaagcttt tcagcaagaa gaagctagtc aacagcaatt
    12721 tgaactgcta aatgctgatc tatatgaaaa aggacaacgg gcacaatttt cttcttcctt
    12781 gacaaacccg tcttctcgtt ttgtagatca ccttgcttat ttatcgattg gtttagttgg
    12841 cggcctattg gcattgaaga gtggttcgtc aatcactgtt ggtgtgattt ccagctttac
    12901 gatctactca agtcagtttt ctaaaccatt tatcgaactt tctggaatca cgacacaact
    12961 gcaaaccgct ttttctggtc tagaacgagc atttgatttg atcgatcagc cagaagaaaa
    13021 gcctgatttg ccagatgcct atgccttgga acccaaaaat atcaaaggaa aagtctcttt
    13081 tgatcatgtt tctttttctt atgaccccaa gcagcgtttg atcgaagatt tcaattttac
    13141 tgcttatcca ggtcaaacca ttgctatagt aggaaaaaca ggggccggaa aatccacact
    13201 tgttaatttg ctgatgcgtt tttatgacac aacaaaagga acaattacag tcgatgacca
    13261 tgacatccgc tctatccaac gtgatacatt acgtaaaagt tttggaatgg tgttacaaga
    13321 tacttggcta tttgacagta cattgcgaga aaatcttcgt tttgggaatc cggaagcaag
    13381 tgacgcagaa atcaatgatg ccctaaaagc ctcttatatg tatgaatttg tagaacgttt
    13441 gccggaaaaa ttagatacta agattggcgg tcaagggtta aagatatcag atggacaacg
    13501 ccaactgttg acgattgctc gaacgatgat cagtaatccc cctatgttga tcttagatga
    13561 agctaccagc tcagtcgata cattgacaga gaaaaaaatc caacaagcct tcttagcaat
    13621 gatggaagga aaaaccagtt ttgtgattgc ccatcgcctg tccacaatca aaaatgcgga
    13681 ccagatatta gtgatggatc agggagctat tgtcgaaatg ggcacccatg atgagctgct
    13741 tcaaaaagat ggttattacc gacggctcta tcaagcacaa ttcacagcac aataaaaaca
    13801 agcagtcttc tgggatggga agactg
//
LOCUS       DACCQP010000069        13807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCQP010000069 DACCQP010000000
VERSION     DACCQP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.071"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO87_002168"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO87_002168"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7450331.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVO87_002169"
     CDS             complement(2260..2511)
                     /locus_tag="IVO87_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7450332.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVO87_002170"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVO87_002170"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7450333.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IVO87_002171"
     CDS             3461..3679
                     /locus_tag="IVO87_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450334.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IVO87_002172"
     CDS             complement(3968..4480)
                     /locus_tag="IVO87_002172"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7450335.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO87_002173"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO87_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7450336.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IVO87_002174"
     CDS             complement(6933..7367)
                     /locus_tag="IVO87_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7450337.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IVO87_002175"
     CDS             complement(7377..8165)
                     /locus_tag="IVO87_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7450338.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IVO87_002176"
     CDS             complement(8162..9103)
                     /locus_tag="IVO87_002176"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7450339.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IVO87_002177"
     CDS             complement(9280..9867)
                     /locus_tag="IVO87_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7450340.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO87_002178"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO87_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7450341.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IVO87_002179"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IVO87_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7450342.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IVO87_002180"
     CDS             complement(12784..13719)
                     /locus_tag="IVO87_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7450343.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DACCQP010000070        13732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCQP010000070 DACCQP010000000
VERSION     DACCQP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.072"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(63..1268)
                     /locus_tag="IVO87_002181"
     CDS             complement(63..1268)
                     /locus_tag="IVO87_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7450344.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IVO87_002182"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IVO87_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IVO87_002183"
     CDS             complement(1447..2832)
                     /locus_tag="IVO87_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7450345.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IVO87_002184"
     CDS             complement(2861..3247)
                     /locus_tag="IVO87_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7450346.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IVO87_002185"
     CDS             complement(3263..3577)
                     /locus_tag="IVO87_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7450347.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IVO87_002186"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IVO87_002186"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7450348.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IVO87_002187"
     CDS             4837..6126
                     /locus_tag="IVO87_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7450349.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IVO87_002188"
     CDS             complement(6165..6770)
                     /locus_tag="IVO87_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7450350.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IVO87_002189"
     CDS             complement(6966..7430)
                     /locus_tag="IVO87_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7450351.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IVO87_002190"
     CDS             complement(7452..7772)
                     /locus_tag="IVO87_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7450352.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IVO87_002191"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IVO87_002191"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7450353.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IVO87_002192"
     CDS             9194..9544
                     /locus_tag="IVO87_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7450354.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IVO87_002193"
     CDS             9729..10682
                     /locus_tag="IVO87_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7450355.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IVO87_002194"
     CDS             10695..12482
                     /locus_tag="IVO87_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450356.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IVO87_002195"
     CDS             12469..13671
                     /locus_tag="IVO87_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450357.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2519 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg ga
//
LOCUS       DACCQP010000071        13488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCQP010000071 DACCQP010000000
VERSION     DACCQP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.073"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(164..388)
                     /locus_tag="IVO87_002196"
     CDS             complement(164..388)
                     /locus_tag="IVO87_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7450358.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IVO87_002197"
     CDS             complement(586..2166)
                     /locus_tag="IVO87_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7450359.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IVO87_002198"
     CDS             complement(2567..3943)
                     /locus_tag="IVO87_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7450360.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IVO87_002199"
     CDS             complement(4071..5189)
                     /locus_tag="IVO87_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7450361.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IVO87_002200"
     CDS             5331..5765
                     /locus_tag="IVO87_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450362.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IVO87_002201"
     CDS             complement(5843..6376)
                     /locus_tag="IVO87_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7450363.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVO87_002202"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IVO87_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7450364.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVO87_002203"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IVO87_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7450365.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IVO87_002204"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IVO87_002204"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7450366.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYVLPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IVO87_002205"
     CDS             complement(10101..10679)
                     /locus_tag="IVO87_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7450367.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="IVO87_002206"
     CDS             11001..11471
                     /locus_tag="IVO87_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7450368.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="IVO87_002207"
     CDS             complement(11579..12805)
                     /locus_tag="IVO87_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7450369.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3959 a         2718 c         2248 g         4563 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgt actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DACCQP010000072        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCQP010000072 DACCQP010000000
VERSION     DACCQP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.074"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IVO87_002208"
     CDS             complement(<1..914)
                     /locus_tag="IVO87_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7450370.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IVO87_002209"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IVO87_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7450371.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IVO87_002210"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IVO87_002210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7450372.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IVO87_002211"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IVO87_002211"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7450373.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IVO87_002212"
     CDS             4440..5321
                     /locus_tag="IVO87_002212"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7450374.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IVO87_002213"
     CDS             5318..6034
                     /locus_tag="IVO87_002213"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7450375.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IVO87_002214"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IVO87_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7450376.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IVO87_002215"
     CDS             6751..7752
                     /locus_tag="IVO87_002215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7450377.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IVO87_002216"
     CDS             7749..9110
                     /locus_tag="IVO87_002216"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7450378.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IVO87_002217"
     CDS             9070..10221
                     /locus_tag="IVO87_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7450379.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IVO87_002218"
     CDS             10196..11365
                     /locus_tag="IVO87_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7450380.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IVO87_002219"
     CDS             11540..12919
                     /locus_tag="IVO87_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7450381.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IVO87_002220"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IVO87_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4486 a         2340 c         2922 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcaaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACCQP010000073        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCQP010000073 DACCQP010000000
VERSION     DACCQP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.075"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            147..1016
                     /locus_tag="IVO87_002221"
     CDS             147..1016
                     /locus_tag="IVO87_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7450382.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IVO87_002222"
     CDS             1225..2250
                     /locus_tag="IVO87_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7450383.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IVO87_002223"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IVO87_002223"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7450384.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IVO87_002224"
     CDS             3333..5036
                     /locus_tag="IVO87_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7450385.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IVO87_002225"
     CDS             5038..7071
                     /locus_tag="IVO87_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7450386.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IVO87_002226"
     CDS             7082..7522
                     /locus_tag="IVO87_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7450387.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IVO87_002227"
     CDS             complement(7722..8849)
                     /locus_tag="IVO87_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450388.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IVO87_002228"
     CDS             complement(8852..9529)
                     /locus_tag="IVO87_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450389.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IVO87_002229"
     CDS             9793..11559
                     /locus_tag="IVO87_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7450390.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IVO87_002230"
     CDS             11574..12011
                     /locus_tag="IVO87_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7450391.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IVO87_002231"
     CDS             12024..13175
                     /locus_tag="IVO87_002231"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7450392.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2730 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgttta catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACCQP010000074        13183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCQP010000074 DACCQP010000000
VERSION     DACCQP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.076"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVO87_002232"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVO87_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7450393.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVO87_002233"
     CDS             1995..4661
                     /locus_tag="IVO87_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7450394.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVO87_002234"
     CDS             5025..5777
                     /locus_tag="IVO87_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7450395.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVO87_002235"
     CDS             complement(5799..6953)
                     /locus_tag="IVO87_002235"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7450396.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVO87_002236"
     CDS             7138..9579
                     /locus_tag="IVO87_002236"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7450397.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVO87_002237"
     CDS             complement(9909..10613)
                     /locus_tag="IVO87_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450398.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVO87_002238"
     CDS             complement(10800..11045)
                     /locus_tag="IVO87_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450399.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVO87_002239"
     CDS             complement(11062..11913)
                     /locus_tag="IVO87_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7450400.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVO87_002240"
     CDS             12079..13083
                     /locus_tag="IVO87_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7450401.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4209 a         2341 c         2754 g         3879 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtg
//
LOCUS       DACCQP010000075        13179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCQP010000075 DACCQP010000000
VERSION     DACCQP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.077"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..975)
                     /locus_tag="IVO87_002241"
     CDS             complement(<1..975)
                     /locus_tag="IVO87_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7450402.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGL"
     gene            1130..3364
                     /gene="pcrA"
                     /locus_tag="IVO87_002242"
     CDS             1130..3364
                     /gene="pcrA"
                     /locus_tag="IVO87_002242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7450403.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            3381..5417
                     /gene="ligA"
                     /locus_tag="IVO87_002243"
     CDS             3381..5417
                     /gene="ligA"
                     /locus_tag="IVO87_002243"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7450404.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            5523..5828
                     /gene="gatC"
                     /locus_tag="IVO87_002244"
     CDS             5523..5828
                     /gene="gatC"
                     /locus_tag="IVO87_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7450405.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            5828..7300
                     /gene="gatA"
                     /locus_tag="IVO87_002245"
     CDS             5828..7300
                     /gene="gatA"
                     /locus_tag="IVO87_002245"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7450406.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7297..8727
                     /gene="gatB"
                     /locus_tag="IVO87_002246"
     CDS             7297..8727
                     /gene="gatB"
                     /locus_tag="IVO87_002246"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7450407.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            8737..9792
                     /locus_tag="IVO87_002247"
     CDS             8737..9792
                     /locus_tag="IVO87_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7450408.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            9903..11276
                     /gene="rlmD"
                     /locus_tag="IVO87_002248"
     CDS             9903..11276
                     /gene="rlmD"
                     /locus_tag="IVO87_002248"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7450409.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11279..11407
                     /locus_tag="IVO87_002249"
     CDS             11279..11407
                     /locus_tag="IVO87_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7450410.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            11847..12035
                     /locus_tag="IVO87_002250"
     CDS             11847..12035
                     /locus_tag="IVO87_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7450411.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12113..13126
                     /locus_tag="IVO87_002251"
     CDS             12113..13126
                     /locus_tag="IVO87_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7450412.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
BASE COUNT         4564 a         2377 c         2871 g         3367 t
ORIGIN      
        1 aagaccccca ccgtaagaaa tcatggaaaa aacgaatcga agaacaaaca aaccgataat
       61 cataatgatt ggcaaggaag catgtccaaa agctaaaacg atttgattgc ctccgcctag
      121 agtactaggc cataaataac caattggcaa aatcagcaag aaaggaacga acccgtgaaa
      181 ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa gccagcaaga cattctgata
      241 aataaaacct aagatgccta gaagtactcc taagatcact aaggaaccat aatatttcaa
      301 cggcaaagaa ggaaggtcac ctagatataa aacaggtctc aagccgaaaa agtaaagaga
      361 gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc caaacaagtg gagaaaagga
      421 gtgatggatt tcttctaaaa caaagagtaa tcctgcgata ggagcattga atgctgctgc
      481 aagtccgcta cttgcaccgc tagaaattaa aatcttttct tctgaagatg gactccgaaa
      541 caattgtcca gctccttgtc caatggatgc gccaagctgt atagaaggtc cttctctacc
      601 tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg aactttttcc ataaaacaga
      661 gaaccagttc attgataatt ccccgcgaag ctgtccctct acttgtggaa tcccgcttcc
      721 cttgatatcc ggttcatttt tgaccagata acccacgaaa aatgctgcaa ggatgcagca
      781 aatagctagg ccaattaagt aaatcggctg ttcccgactc atttcataga tacttcgaac
      841 taaatcgctt agcttttcga tagataatcg aaacaggcta acgacgattc ctgtcagcag
      901 tccaacaaat attcctttta gaataaaata tatctgagtc ttatctaatt tcttgatttt
      961 tccttttttt tccattcaaa cgcctcgctt tcttttaatt tagcgcgaaa tcaacgaatt
     1021 gacaaggaaa aatccataaa aagaaacaaa tgattttgaa ctagctagtt taatgaaagc
     1081 caagaaaatg ctataattaa caagattgat aaaagaaagg atgttgttaa tgtcatccaa
     1141 agctgaatta ttaaacggga tgaacccccg tcagaaagaa gctgttcttc acactgacgg
     1201 accattactg ctaatggcag gtgcaggaag tggaaagacg agagtgctga cacaccgcat
     1261 tgcctatttg attgaggaaa aagaagtcaa tccttggaac atcctagcga ttacttttac
     1321 caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg attctcgcat ctggaggcga
     1381 agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt attttaagaa gagatgttga
     1441 ttttatcggg tataaccgga actttacaat catcgattct tctgaacagc tgaccttgat
     1501 gaaacggata ctaaaagaac tgaacattga tccgaaaaaa tatgatcctc gaagtatatt
     1561 aggaacaatc tctcaagcaa aaaacagcct gcagacccct caggattttg caaaaatgca
     1621 aggcagttat tacgaagaga ttgcggcaaa atgttatgct gcttaccaaa aagagcttca
     1681 atataatcaa tgtatggact ttgacgacct gatcatgaat acgattcgat tatttgaaga
     1741 acatcctgat tcactcacgt attatcaaaa caaattccat tatattcatg tagatgagta
     1801 ccaagataca aatcacgcac agtatacgct agtcaattta ttggcaggta gatttaggaa
     1861 cctgtgcgtc gtgggagatg cagatcaaag tatttacggc tggcgcggtg cagatatgca
     1921 gaacatcttg gattttgaaa aagattatcc agatgctgca gtaattttgc tagaacaaaa
     1981 ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa gtcattgaaa acaatagcaa
     2041 tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga aacaagatca cttactaccg
     2101 tgcagataat gaacgagacg aaacccgttt tatcgtagac cggatgcaag aagagattcg
     2161 aagcaatcat cggaattacg gcgactttgc catcttgtac cggacgaacg cacaatctcg
     2221 tgtaatggaa gaaactctgt tgaaagcaaa tattccttat aaaatggttg gagggcacaa
     2281 gttctacgat cgtaaagaaa tcaaagatat tttagcgtat ttgaatgtgc tggtaaatcc
     2341 tcaagattca atcagttttg agcgtatcgt taattcgccc aaacgaggca tcggaccagg
     2401 atctatcgaa aaactacgca gttttgcttc tcttcatgaa tggccactgc ttgaagcagc
     2461 gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga cagcaattag gtgcatttgg
     2521 agagatgatc caagaagtca ctcagatgat cccatacttg acagtgacag aattgacgaa
     2581 agaggttttg gatcgtagcg gatatctaga agacttaaaa atccaaaata cattagaagc
     2641 acaagcacgc atcgaaaact tggaggaatt tttaacggtc acacaagaat tcgacaaaca
     2701 attcgaacag caaaatgaag aagacgctga tgcaccagaa gaaaaactaa cggtcttctt
     2761 aaatgatctg gcacttgttt ccgacattga caatttagaa gaagatgctt cccaagtgac
     2821 attgatgact ttgcatgcag ccaaaggatt agaatttcca gttgtattcc tgattggtct
     2881 agaagagggg gtttttcctt tatctcgagc attgatggaa gaaagcgaac tggaggaaga
     2941 acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa gcgttgtatt taacaaatgc
     3001 tttctccaga acattatatg gacggacgca atataaccga ccttctcgtt ttgtagaaga
     3061 aatcgatcag gagttactgg aaatcgaagg catgcgtcct acgcctaaaa aaacgcctgt
     3121 attcgctaaa aaaacagcat atagctataa acagccagag acagcggttg ttccctctaa
     3181 aagtgctact ggaggggaaa ataacagctg gaaacctgga gataaagtca aacacaaaaa
     3241 atggggtcta ggaacagtcg tccgagtaag cgggacgtcg aaggatctag aactagatgt
     3301 ggctttccca agtcaaggag taaaacgtct tttggctgcc tttgcgccaa ttgaaaaagc
     3361 ttagtagtgg agggtaaatg atggaacatg aaatgacgat tgacgaagct gctaaacgag
     3421 cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga atactacgta gaagataaac
     3481 caacagtgga agactatgtt tatgataaag aatacgcaga attagtagca atcgaggaac
     3541 aatatcctga tttgatcacg tctgattctc ccactcaacg agtaggcgga aaagtattag
     3601 aaggttttga aaaagtcact catgatattc cgctttacag tttgaacgac gttttcagca
     3661 aagaagagct gatagctttt gatcaacgtg tacaaaaagc agtgggacgt gtagttgact
     3721 attgctgtga actaaaaata gatggacttt ccgtttcttt gcgttatgaa gatggaaatt
     3781 tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga aaacatcact gaaaacctaa
     3841 aaactgtccg gtcggtccca atcaaattaa aagaaccaat gaacatcgaa gtaagaggcg
     3901 aatgtttcat gccaaaacgg tcatttgtcc aattgaacca agacagagaa gcagagggaa
     3961 aagatatctt tgcgaatcct cggaacgccg cagctggttc tttgcgtcag ttagattcta
     4021 aaatcacagc gaaaagaaat ctggacactt ttttatacac agtagctgat ttcggtccta
     4081 tgcaagcaaa aacccagtat gatgcgctag aagagctaga aaaaattgga ttccacacga
     4141 atcgggaaaa acgtctatgt cattcgattg atgaagtgtg gtcatacatt gaagaatatc
     4201 atgacaaacg tgtggatttg ccctatgaaa tcgatggaat cgtcatcaaa gtgaatgaat
     4261 tctcgctaca agatcaactt ggttttacta tcaaagcacc gagatgggca gcagcttaca
     4321 agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat cgaatggaca gtcggtcgaa
     4381 caggggtagt aacgccaaca gctatcatga caccagtccg agtagcagga acgacagtaa
     4441 gccgtgccag cctgcataac ggtgattata ttaagttgaa ggatatccgc ttgaaagata
     4501 cagttctgat ttataaagcg ggagacatca tcccagaagt ttcgcaagtc gtgttagata
     4561 aacgacctaa agacagcgaa gaataccaat tgcctacaca ttgtccagtt tgtggaagtg
     4621 aacttgtcca tttggatgaa gaagtagcct tacggtgcat caatcctaaa tgccctgctc
     4681 aaatgaaaga aggtttgaac cactttgttt caagaaatgc gatgaatatc gatgggttgg
     4741 ggccgcgcgt actagaacag atgtatgata aaaaactagt agcagatgtt gctgatcttt
     4801 acaaattgac agaagaagag ttattgacgt tggacaaaat aaaagaaaaa tcagcaaata
     4861 atattttgac agcaatcgac aacagcaaag ataattcggt cgagcggttg attttcgggt
     4921 taggaatccg acatgtcgga gcgaaagcag caaaaatttt agcagaacat ttcggtgatc
     4981 tagaaacatt gagtaaaagt gactatgagt cgattattgc gcttgatacg atcggtgata
     5041 tcattgccga cagtgtagtc acttatttca gtaacgaaga agtccatgaa ttgatgaatg
     5101 aattaaaaca agcaggcgtc aatttcgaat acaaagggct tcgaaatgcc caattgcaag
     5161 aagtcgagtc tccttttaaa gaaaaaacag tagtccttac tggtaaacta acacgattta
     5221 ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg gaaagtgact ggtagcgtat
     5281 cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg cagcaagctg accaaagcac
     5341 aagaattagg aatcgaagtt tggacagaag atcaaatggc agatgcattg gctaaaagtc
     5401 gctcagtaga agaatgaaaa atgagttttt tcagacaaat ttcaataaac aatttcagac
     5461 acggcactct tgtgttaaaa tagagagcaa agtttttaac tcgtttagaa agaaggggta
     5521 gtatggcgat tagtgaagaa caagtgaaac acgttgccaa attagcaaaa cttgcgttcg
     5581 cagatgaaga acttgcaagt tttaccgacc aattaggtaa aatcatcgat atggttgaaa
     5641 tgctggaaga agtagatacg gaaggtgttc catttacgtc gaatgtggtt gaaacagtaa
     5701 acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg cgatgaatta ttgaaaaatg
     5761 tgccggaaac agaagacggc tttatcaaag tacccgcaat catcgataat ggggaggctg
     5821 gcgcataatg acagaattac acaatcgttc tctagaagaa ctgcatcata tgcttgtatc
     5881 gaaagaaatc actgtacaag atttgacaaa aacaacattt gaacgaatca aagaaacaga
     5941 gcctgaggtc gaggcattca ttacattgaa cgaagaaaaa gcattggaac aggcaaaagc
     6001 tttagatgaa aaagggattg atgaaagcaa cgtccttgct ggtattccaa ttggtatcaa
     6061 ggataatatc gtaacaaaag atatcttgac aaccgctgca tctaagatcc tttacaattt
     6121 tgaaccaatt tatgatgcaa cggtcatgga taaaatctat caagcagata tgatccctgt
     6181 tggtaagcta aacatggatg aatttgctat gggtggaagt acagaaactt cttatttcaa
     6241 gaaaacaaaa aatgcttggg acccaacaaa agtacctggt ggctcttctg gtggttctgc
     6301 agcagcagtt gcagctggac aaattccagt ttctttaggt agtgatactg ggggaagtat
     6361 ccgccaacca gcagctttta acggcatcgt agggatgaaa cctacttatg gccgtgtctc
     6421 acgttttggt ttgattgcct ttgcttcatc tcttgaccaa atcggaccaa tgacccgtac
     6481 ggtgaaagat aatgcattag cattgacagc tatcagcggt tatgacgaaa aagacggtac
     6541 ttcttcaggc gtttcagttc ctgattttgc agagggactg acaggcgaca tcaaaggaat
     6601 gaagattgct ttacctaaag aatacttagg cgaaggtgtg gcccctggcg taaaagaagc
     6661 tgtattgaaa gcagcagaaa cattccgttc attaggtgct gttgtggaag aagtcagctt
     6721 gcctcattca aaatacggtg tggctgttta ttatatcatt gcctcatcag aagcaagttc
     6781 caatttgcaa cgattcgatg gtatccgcta cggttatcgc tctgaaaatg tgaaatcatt
     6841 agatgatgta tatgtcaact cacgttcaga aggattcggt gaagaagtaa aacgccgtat
     6901 catgttaggt acattctcct taagcgcggg ttattatgac gcttacttca aaaaagcagg
     6961 acaagtccgt acattgatca aacgagattt tgaaaaagtc tttgcagatt atgacttgat
     7021 catcgggcca tcttcaccaa cagttgcatt cggaatcggt gagaacatta acgatccaat
     7081 cacaatgtac atgagtgaca tcttaacgat ccctgtcaac ttggctggac ttccaggtat
     7141 gtctgtaccg gcaggtttat cagaaggatt accagttgga ttgcagatca tcggtaatta
     7201 ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt gaacaagcaa cagaattcca
     7261 taaacaacaa ccagctattt taggagggaa agacgaatga attttgaaac agtcattgga
     7321 ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt tctctccagc gccagctcat
     7381 tttggtgcag aaccaaacag caatacaaac gtgatcgact ggggttatcc aggcgtgctg
     7441 ccagtaatga ataaagctgc gctggaattc gggatgaaag cagctttggc attaaactgt
     7501 gaaatctcta aagatactca ttttgaccgg aaaaattact tttacccaga taatccaaaa
     7561 gcatatcaaa tttcccaatt tgatcaacca atcggtcatg acggatggat cgaaatcgaa
     7621 gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc atttagaaga agatgctgga
     7681 aaaaacatgc atggtattgg cggttattct tacgttgatt tgaaccgaca aggcacgcct
     7741 ttgatcgaaa tcgtttctga agctgatatg cgttcaccag aagaagcata cgcttatcta
     7801 gaagcattgc gatcaattat ccaatttaca gaagtcagcg atgtgaaaat ggaagaaggc
     7861 tctatgcgct gtgatgccaa tatttctctt cgcccttatg gacaagaaga gttcggtacg
     7921 aaagcagaat tgaaaaactt gaactcttta tctttcgtga aaaaaggttt agcttttgaa
     7981 gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa tccaacaaga aacacgccgt
     8041 tttgacgaaa caacgaataa aacagttttg atgcgtgtca aagaagggtc tagtgattat
     8101 cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg atgacgaatg gattcaaaaa
     8161 gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg aacgctatat ccgtgagttt
     8221 ggacttccag agtatgacgc aatggtattg acacttacaa aagaaatgtc tgattttttt
     8281 gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat ctaactggtt gatgggtgaa
     8341 gtttctgctt acttgaacag tgagaaactg gaattagctg agacaaaatt gacacctgag
     8401 aacttagcag gaatgattcg tttgatcgcc gatggaacga tcagctcaaa aatcgccaaa
     8461 aaagtcttcc gtgaattgat cacaaatggc ggagaagccc aagaagtagt tgaaaaaaat
     8521 ggttgggtgc aattatctga tccgaacaaa ctactgccaa tcattaatga aattttggat
     8581 aacaaccaac aatctgtcga tgatttcaaa aatggaaaag atcgtgcggt tggcttccta
     8641 gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc ctggtgtcgt gaataagcta
     8701 cttaaagatg aattagcgaa acgatagagg ctgaaaatga aaaaagcacg cgtaatttat
     8761 aaccccacct cgggaaaaga attattgaaa aaaaatttgg cggatatcct acaggcatta
     8821 gaagaagcag gatttgaagc cagcgcctat gcaactacac cagaaccaga ttcagccaag
     8881 aatgaagcat gtagagtcgc agaactaggt tttgatctag tagtggcagc tggtggcgat
     8941 ggcacaatca acgaagtggt taatggaatc gctccgcttg aacaccgtcc taaaatggcc
     9001 atcatcccag ctggtacaac gaacgactat gctcgagcat taaaaattcc tagagataat
     9061 attaaagcag cagcagaagt gataaaaaaa caccaaaccg taaaaatgga catcggcctt
     9121 tctgataaca gttacttcat caatatcgct gcaggtggct atttgacaga attgacttat
     9181 gaagttccat cagaactaaa gagtattttt ggttatcttg cttatcttgc gaaaggagca
     9241 gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga agtatgatga aggggaatat
     9301 atcggaaatg cttcgatgtt tttcctaggg ctgacgaact ctgttggagg attcgaacag
     9361 atcgcgccag atgccaaatt ggatgatggg aagttttctt taatcattgt gaagacagca
     9421 aatatctttg agatcttgca tattgcagca ctcatgctga atggcggtcg acatgtcgat
     9481 gatccaagaa taatctatac caaaacaagc agtttgttcg ttgaaacaga agatgatatc
     9541 aatcgaccag taatgatcaa cttagatggt gaatacggcg gggaagcacc gatgcatttc
     9601 caaaatcttc atcaacacat tgagttcttt gccaataccg atcagattcc tgatgaagca
     9661 atcacaggta cagatgaaga agaattagag gttgtcagca aagaattcgt aaaagaagtc
     9721 gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa tagcagacga aacacctaat
     9781 caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg tcagtgccgg ttttagttgt
     9841 gtaattagct gatttatagt agagttcagt aggattgaaa aataggctta aaggagcaaa
     9901 atatgacaga acagcaaata gtaaagaaaa acgaacgata tacagtcgaa atcgtcgact
     9961 tgagttatga aggaatgggg gtagcaaaaa tcgatggtta tccattattt atcgaaaatg
    10021 cactccctgg agaagaagta gaaatactag tagtaaaagc aggcaataag tttggatatg
    10081 gaaaagtcga aacatttatt acacaatcac ctgatcgtca gcctgtagaa aacaatgcat
    10141 tacttcgcat aggtatcgca cctttagccc atttgaagta tgaacaacag cttgtcttca
    10201 aacaaaaaca agtagagcat gtaatggcga agattgctaa gatgccagaa gttcctgtac
    10261 ttccaacggt cggaatggca gaaccattcg gctaccgtaa taaagcacag atccctgttc
    10321 gccgtatcga tggacagtta gccaccggtt tttacaaaaa gaatagccat gatctagtcc
    10381 ctatcgaaga tttttatatc caagatcctg tcatcgatca agcaatcaaa gtagtcagag
    10441 atatcttgca acggttccaa gtacgaggat ataatgaaga aaaaaatgaa ggacagcttc
    10501 gtcacatcat tatccgtcgt ggtcattatt cccatgaaat gatggtcgtt ttagttactc
    10561 gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc tattcatgaa gaattgccag
    10621 aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac aaatgtgatt ttaggagaca
    10681 aagaaaaagt tttatatggc agagactata tcgaagatca gctattagga aaaacctatc
    10741 gaatctcagc aaagtcattt tatcaagtaa atactcaaca agcagagtat ctgtaccaaa
    10801 cagccatcga gtttgccgag ttagataaaa aagacattgt gatcgacgct tattcaggaa
    10861 taggtacgat tggcttgtct ttagcagaca gagtggataa ggtgtatggg atggaagtta
    10921 ttccagaagc aattgaagat gcacagttca atgctttaaa taataaaatc gaaaatgttc
    10981 attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg gcaagaaaaa gggatcaaac
    11041 catctgtgat cgtagttgac cctccacgaa aaggattaga tcatcatttt attgaatcag
    11101 caatcgaaat gtcaccagaa cgaatcgtct atatctcatg taatccggca acttttgcaa
    11161 gagatgctaa gttatttgca gaagcaggtt atgccgtgaa gaaagttcag cctgtagatc
    11221 ttttcccgca aacccatcat atcgaattag tcgcttcatt cgttaaagga gaataaaaat
    11281 ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa caaggtggag gccgatccaa
    11341 acaaatggaa acacctaaga atgatcgtaa aaacatgaga aaaggcccta aaatctttaa
    11401 taaatagacg tgctaaagaa agactaagaa gtgttaactt ttgttattgt ttcttagtct
    11461 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
    11521 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
    11581 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
    11641 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
    11701 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
    11761 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
    11821 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
    11881 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
    11941 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
    12001 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
    12061 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
    12121 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
    12181 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
    12241 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
    12301 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
    12361 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
    12421 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
    12481 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
    12541 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
    12601 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
    12661 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
    12721 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
    12781 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
    12841 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
    12901 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
    12961 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
    13021 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
    13081 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
    13141 gccgtttcgt tttgagtatg aagaaacgaa gcggctttt
//
LOCUS       DACCQP010000076        13057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCQP010000076 DACCQP010000000
VERSION     DACCQP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.078"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..224)
                     /locus_tag="IVO87_002252"
     CDS             complement(<1..224)
                     /locus_tag="IVO87_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7450413.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKS"
     gene            complement(221..874)
                     /locus_tag="IVO87_002253"
     CDS             complement(221..874)
                     /locus_tag="IVO87_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450414.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1162..2095
                     /locus_tag="IVO87_002254"
                     /pseudo
     CDS             1162..2095
                     /locus_tag="IVO87_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2003..2719)
                     /locus_tag="IVO87_002255"
     CDS             complement(2003..2719)
                     /locus_tag="IVO87_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7450415.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2769..2978)
                     /locus_tag="IVO87_002256"
     CDS             complement(2769..2978)
                     /locus_tag="IVO87_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450416.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3255..4528
                     /locus_tag="IVO87_002257"
                     /pseudo
     CDS             3255..4528
                     /locus_tag="IVO87_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4635..5666)
                     /locus_tag="IVO87_002258"
     CDS             complement(4635..5666)
                     /locus_tag="IVO87_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450417.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5673..6503)
                     /locus_tag="IVO87_002259"
     CDS             complement(5673..6503)
                     /locus_tag="IVO87_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450418.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6500..7306)
                     /locus_tag="IVO87_002260"
     CDS             complement(6500..7306)
                     /locus_tag="IVO87_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7450419.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7312..8136)
                     /locus_tag="IVO87_002261"
     CDS             complement(7312..8136)
                     /locus_tag="IVO87_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7450420.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8126..9226)
                     /locus_tag="IVO87_002262"
     CDS             complement(8126..9226)
                     /locus_tag="IVO87_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7450421.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9404..10189
                     /locus_tag="IVO87_002263"
     CDS             9404..10189
                     /locus_tag="IVO87_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7450422.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10222..10605
                     /locus_tag="IVO87_002264"
     CDS             10222..10605
                     /locus_tag="IVO87_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7450423.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10681..10812
                     /locus_tag="IVO87_002265"
     CDS             10681..10812
                     /locus_tag="IVO87_002265"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7450424.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10781..11017
                     /locus_tag="IVO87_002266"
     CDS             10781..11017
                     /locus_tag="IVO87_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7450425.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11095..12066)
                     /locus_tag="IVO87_002267"
     CDS             complement(11095..12066)
                     /locus_tag="IVO87_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7450426.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12700..>13057
                     /locus_tag="IVO87_002268"
     CDS             12700..>13057
                     /locus_tag="IVO87_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7450427.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVR"
BASE COUNT         3904 a         2941 c         2390 g         3822 t
ORIGIN      
        1 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
       61 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
      121 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
      181 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
      241 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
      301 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
      361 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
      421 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
      481 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
      541 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
      601 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
      661 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
      721 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
      781 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
      841 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
      901 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
      961 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     1021 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     1081 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     1141 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     1201 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     1261 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     1321 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     1381 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     1441 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     1501 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     1561 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     1621 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctagt
     1681 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     1741 cgcagacttt acaaactaac tcaatacttc ctgcactttc ctagaaaagc ccgtttaaag
     1801 gtcaagtacc tagaatgcca gctattgtca gctgcttaaa acggtctttc caaatgcgga
     1861 gatcgtaact gatcgctttc atattgtcca acatatcaat cgcagcttca atcaattgcg
     1921 tatccagatc atgaatcgtt ttcgagcctc gcactctgaa aatcagaaga agtaccgtcg
     1981 gctcaagcga tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg
     2041 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt
     2101 gtgcacaatg gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat
     2161 cacggggatg gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt
     2221 gcggtccgta aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca
     2281 gttcttgctc ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc
     2341 ctgcaatgtt cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt
     2401 aaagattgac aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca
     2461 attgacgttt gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa
     2521 aggcgaaaaa atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg
     2581 caccgactgg actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca
     2641 acaaataacc ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat
     2701 caattttttc cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag
     2761 aattgagact actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc
     2821 actggtctcc caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca
     2881 acaccttgtt gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct
     2941 tcagtaaata gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat
     3001 caaaatcaaa aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag
     3061 ctgtgtcctg ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg
     3121 aaagaaatgt ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg
     3181 agcataaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     3241 aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg
     3301 gaaacattca aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg
     3361 tttctgccac gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac
     3421 atgggctgat cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga
     3481 atcaacggac ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct
     3541 attttacggc gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt
     3601 ataaaatatt ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg
     3661 gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga
     3721 aaataaattg gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag
     3781 tcggtcccta ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc
     3841 cttcacgtaa gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt
     3901 taaacgtaaa aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt
     3961 gctttcctca tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca
     4021 gttttaagga tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag
     4081 ccaaacacta tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc
     4141 ttgtttatga taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg
     4201 aagtcgtaga acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc
     4261 aagaaattct tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa
     4321 caatgcccaa gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg
     4381 aatccggcat tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc
     4441 atacccacat caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt
     4501 cttaatcaat catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat
     4561 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt
     4621 ttgccgacta tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt
     4681 gctagaatat ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc
     4741 aaccgatcca ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg
     4801 cctaaaataa atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca
     4861 aattgaatca gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg
     4921 tgggtatact gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca
     4981 acaaatcggc tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc
     5041 ccagcttcca tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc
     5101 acaaaaatca tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt
     5161 tttctaattc gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca
     5221 gtgaccaatg agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca
     5281 tttttatctg cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga
     5341 atcacctcag gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc
     5401 atagttggaa acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc
     5461 ttatcggcaa tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc
     5521 gtgatacagc gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc
     5581 tcaccctttt caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga
     5641 ttgagatcat gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc
     5701 gactgcttct cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc
     5761 ataaaggtag ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta
     5821 gccaaatggc cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga
     5881 ccgccaatca acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga
     5941 gaaactaggg tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac
     6001 atcggggcgc ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc
     6061 tggttgttaa gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac
     6121 actacaaaca ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc
     6181 actcctggta aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg
     6241 aaatacaaat gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc
     6301 acacctgcta ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc
     6361 tgttggaacc attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag
     6421 ctggcataca aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa
     6481 atcggtttta ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca
     6541 ttgccgttac cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg
     6601 cgaacacatc tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc
     6661 tggtcagggc atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg
     6721 tgctggcaat cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg
     6781 aagcgcccag caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat
     6841 acaaaaacaa gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc
     6901 aagaatacaa attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc
     6961 ccaacaaaat aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg
     7021 aaacaaaggt gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa
     7081 taaacgtccc gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa
     7141 aggcctgtgt gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt
     7201 tagttaaatc actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg
     7261 cacagaagat aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct
     7321 catcaattct tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa
     7381 caatggagcc gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga
     7441 aatcaaataa aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc
     7501 atccatcccg tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag
     7561 gtaaatagcc aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc
     7621 ggtaaatttg tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg
     7681 atactgattc actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc
     7741 actataaaag atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa
     7801 acgattatag ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt
     7861 ttttccgcca gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg
     7921 gtaattacta tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa
     7981 cggacgtgaa ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc
     8041 aactaaatga ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag
     8101 tataactaca atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa
     8161 gctctggaag ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg
     8221 cttcataagc atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc
     8281 gaataggtcg cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt
     8341 cccgtgctag ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc
     8401 cgctaatgat ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt
     8461 cacgataatt tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa
     8521 gatttgctac gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt
     8581 aattcagacc tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata
     8641 cagcaaattg tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct
     8701 cttgcagctc cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg
     8761 aaatggttcc cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa
     8821 tttcctccca atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac
     8881 cgaagctgat accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact
     8941 tagccaattc ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt
     9001 caaggtcgtt ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa
     9061 tctctccttc ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg
     9121 attgcttcgt tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac
     9181 aaaataaacc gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc
     9241 tttcgataat tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt
     9301 tcatgaacaa tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt
     9361 ctaaagcttt tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca
     9421 gcatctccac acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta
     9481 tcagccggag tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa
     9541 agatgacatt ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta
     9601 ttccaccaaa tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat
     9661 cgtggattac ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg
     9721 ccgctttgat tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga
     9781 atattttgat cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca
     9841 ctttgattat ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga
     9901 ggctcagctg ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa
     9961 tgccaagagc ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta
    10021 tcaaagtgtc ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta
    10081 tcagctgggc tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct
    10141 agtcattcaa ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa
    10201 tagaagaaag tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc
    10261 cattatttaa ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg
    10321 ttagcagccg ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg
    10381 gaaaaagttc aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg
    10441 cagtttcatc ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag
    10501 ctgcaattga cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc
    10561 cttggtgatc aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc
    10621 gtttcagttg caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt
    10681 gtgctggtca ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg
    10741 gctgtagcca actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc
    10801 tcgtgtcatt aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc
    10861 tgtttccagc tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa
    10921 tggcgccttg gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt
    10981 gcaaaacaca aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga
    11041 ggactaccgg agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa
    11101 atcgtagcag cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg
    11161 ttcattcgac aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa
    11221 aagctctatt cttgagctgg ctgcagctag tgagtgtctt tcatttatcc ataagataaa
    11281 gcttggattc aataaataaa taaatgtttt ggttaaagct gtgaccagca aatgaggaac
    11341 cgtgcgagcc agttgatctg ccaatgcaaa catttcttta ggtgaaggcg ctggcgtgtc
    11401 tgccaacgga tactccagtc ccaatgcttg gtaaccgtgc ataccatagc agtggtacgg
    11461 tagtaattct aacggtacga accgatcaag atctgccaca aaacaagcaa tcgtcagcag
    11521 ctcggtcagg ttcatattga tacctggaac cacaggaact cgaattcgca cttcgcagtt
    11581 aggatactca attaaaaagc gacgaatatt cttcaaaatt tgttggttat ctaagccggt
    11641 ccattgtttg tggagcttgc tggtaaacat ttttacatcc acaaacaatg tatccacata
    11701 gggagctacc ttttggattt cattataagc accacaaaag ctggtttcca gtacggtatt
    11761 gattccaatg tatttcgatt tttgcaaaat ttctttagca aaatccgcct gaaccagtgc
    11821 ctcgcctccg ctaatggtca ctccgccatc cgagtgaaag taaaaaacag catccttctc
    11881 aatctcatcc attacctctt cagcggtcat ggtctggcca aatccttttt caatctgctt
    11941 gctttgtgat tcgggaatcg aacaccaggc acagcgtaat gggcagccct ttaaataaac
    12001 aacggtccgt aaaccagcac catcatgtag agaaccctgt tctattctta atactgttcc
    12061 ttccatatag atttttgaat tacacactag tttaaactaa aacacacaag gaaaaactta
    12121 ccatttaatc ataaaattag ctcatataaa tatactagga tgtaaaatag actggtcaaa
    12181 atctggtcaa aaaagaggac taatacaggt agtcctcttt tttgttattt taatattcta
    12241 aatcattttt tattggttta ttcccttatt cgatgtagac tcaaatcact atatcactag
    12301 atatcaaacg gcgtcatacg gaatcaaaaa catcaaattt gacctgttaa atggtcattg
    12361 aataacattc aaaatcaata aaacggagtc agacacggag tcaaaaacgc tgagagtaaa
    12421 aaatatatac agccttgtag gtaaagtacc acagggctgt atatttttta tttcagatta
    12481 ccttaaatga ttccatttac tttttatctc attttttcct actagttagt atcaaatact
    12541 ctcctatttt ctcaataaac tatttttgga tacatggtat ctgaaacagc taaaaccttg
    12601 tcattacagc aaaaaatcac gttatccaga ctgtagacaa agtccatttt atggatgatg
    12661 tctacagtct tttcttttat aatagattta tgaaaacgaa tgcaaggagt gatagctatg
    12721 atgtctaaaa acaatacgaa tggtagaaat caatttgcga tgcttacgat tgatgatcta
    12781 gtttctcaag atcatctcgt tcgaaagatt gatgccgcgc ttgattttga atttatttat
    12841 ccaatcgtcg aagccactta ctccgattta ggtcgcccaa gtatcgatcc agtgattctg
    12901 atcaaattgg tgtttattca gtatttattc ggtatccgtt ccatgcgaca aaccattaag
    12961 gaagtcgata ccaatgtcgc ttaccgctgg tttttaggct actccttcga ggagaaaatc
    13021 ccccatttct caacttttgg aaagaattat gttcgcc
//
LOCUS       DACCQP010000077        13017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCQP010000077 DACCQP010000000
VERSION     DACCQP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.079"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            9..2138
                     /locus_tag="IVO87_002269"
     CDS             9..2138
                     /locus_tag="IVO87_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7450428.1"
                     /translation="MLNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVA
                     LIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTR
                     GTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQL
                     GNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVV
                     AERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDR
                     VGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKV
                     EEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTI
                     TSAFDPGSVVKAGTLTAAWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAV
                     KASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGID
                     LPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANG
                     GKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTD
                     PYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPN
                     LLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2324..3133)
                     /locus_tag="IVO87_002270"
     CDS             complement(2324..3133)
                     /locus_tag="IVO87_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7450429.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            3426..4862
                     /locus_tag="IVO87_002271"
     CDS             3426..4862
                     /locus_tag="IVO87_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7450430.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            5032..6228
                     /locus_tag="IVO87_002272"
     CDS             5032..6228
                     /locus_tag="IVO87_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7450431.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(6290..6475)
                     /locus_tag="IVO87_002273"
     CDS             complement(6290..6475)
                     /locus_tag="IVO87_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7450432.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            6706..10020
                     /gene="dnaE"
                     /locus_tag="IVO87_002274"
     CDS             6706..10020
                     /gene="dnaE"
                     /locus_tag="IVO87_002274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7450433.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            10187..11149
                     /gene="pfkA"
                     /locus_tag="IVO87_002275"
     CDS             10187..11149
                     /gene="pfkA"
                     /locus_tag="IVO87_002275"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7450434.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            11223..13007
                     /gene="pyk"
                     /locus_tag="IVO87_002276"
     CDS             11223..13007
                     /gene="pyk"
                     /locus_tag="IVO87_002276"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7450435.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
BASE COUNT         4141 a         2332 c         2756 g         3788 t
ORIGIN      
        1 tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca caagaaccta
       61 agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt tttttcatta
      121 tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc gtgaacggcg
      181 aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa agtactcctc
      241 gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg aatcaggcca
      301 tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc gcaacgaagc
      361 tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat aaaaaagatt
      421 tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg gcaaaagaaa
      481 agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca gctgatgaga
      541 ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg aatagtgcat
      601 atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag ttagctgttg
      661 tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg acacgagaat
      721 ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa aagcaaggac
      781 ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat gaccgtgtag
      841 gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa acccaatatg
      901 aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc tctggggaaa
      961 aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta gaagaaattt
     1021 tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg cctggtgctt
     1081 atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt ttcagtcatg
     1141 aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc gcctttgatc
     1201 cagggtctgt agtcaaggct ggtactttga cagcagcctg ggcaagtaac gcgatcagcg
     1261 gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa aaaagctctg
     1321 tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa ttgtcttcta
     1381 atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca cctggaatgg
     1441 gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac tctttcaaag
     1501 agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct gggatctcac
     1561 gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg tatgctccag
     1621 gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc caattagctc
     1681 aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta gtcaaagcga
     1741 tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt ggcacaacag
     1801 tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa ggattccacc
     1861 aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt gcgaaaatgg
     1921 acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca gatattgcga
     1981 cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt gccatcagtg
     2041 ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt gctaaagaaa
     2101 taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt gaaaaagccg
     2161 cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt ttgcttaaat
     2221 ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa aaggttgtga
     2281 caaaaattgt cacaacctct tttttgtgga atctaattct ctattatagt ttaagtagtt
     2341 tttccagata aacagctaaa ccgtcttcct gattgctcaa aggagtgaca tcatttgcaa
     2401 tgcctttgag ttggtcagta ccgttagcca tagctactcc ccaacctgca taatccaaca
     2461 attccaaatc attatgctcg tcaccaaagg caatgacgtc tttttgatcg atagacaggt
     2521 agttagcaat ctcttgcacg ccttttgctt tttggattcc ttttgaaaca atctctaaga
     2581 tggcagtagg tccaccccat gtccgaacat cgacataact gccaaattga cgtgttaatt
     2641 cagcagatac agctcctgca aaacgtttgt ctgttcggac aagcaaagat gtaggatttg
     2701 tcgtcaaatt tttaggagaa agcaaattct tctctcctgg acgagaagaa gcaaaaattt
     2761 tttcatcaaa gaaatcaaaa ctgtcaataa aaaaagtatc acgattttca gcagcgataa
     2821 aatctaaatt tagctgcttt ttttgcgata agatttcaaa agcgatttca cgattgatca
     2881 acgtttctct ttcaccatcc cagtgctgat tagggatgtg tactaatgcg ccgttaaaat
     2941 taaccatcgg tgttgttaat tctaattgtt ggtaaaactg atggctcatt cggaatgggc
     3001 gtcctgtagc aatactgaca tgatgaccgg cattgattgc tttttttaga gtctcttttg
     3061 ttttagatgt aatcatcgaa tcttgattga gggtagttcc gtccagatcg atggcaatta
     3121 attttttatt caataagggt ccttccttcc tcaactagca tttgttttag tctagcatat
     3181 tttaggtaat taacaagagc aatcagctat tcctaacaaa aactacagtt atggctatat
     3241 aaatagctaa acataagcta aacacgaacg ttaagtatta ttttcgcttt ttttgttaaa
     3301 aaaacgttga aaagcgaatg aaacgtctat ataatttgaa attgttcgat ggatttgccc
     3361 tattttactt attttttatt attatagttt tccatcagaa aaaaattcag ggagtggaac
     3421 tttttatgga aaagtttttc ggtttaaaga aaaacaatac aaatgtatca actgaaataa
     3481 tggcgggagt aacgacattc tttgccatga gttatatctt attcgtaaat ccgacgattt
     3541 tgtcagcatc aggtatgccc ttccaagcag tatttctagc gacaatcatt gcatcgatta
     3601 ttggtacgct ggtaatggga ttgttcgcaa atgtaccata tgctcaggca cctggtatgg
     3661 gattgaacgc atttttcact tttacggtcg tttttgggct tggttattct tggcaacaag
     3721 ccttggcaat ggtgtttatt tgcggattaa tcaatatttt tattactgtc acaaatattc
     3781 gaaaaatgat tattcgggcg attcccgaaa gtcttcaaca tgcgattggt ggcgggatcg
     3841 gtattttcgt tgcgtatgtc ggaatcaaaa atgcaggatt cttatctttt tcagcagacc
     3901 agtcagctat ttcgagctct gtcgttgaag gcggaaaagc gacaaatgtt acgataaatg
     3961 gtggaatcgt ccctgcgtta gccaactttg acaacgctcc tatcttattg gctgtgattg
     4021 gtttggtact gacagccatt ttagttgtaa aaaatgtccg tggggctatt cttatcggta
     4081 tcgtagcgac cacagtcctt ggtattttta tgggtgttgt tgatttaagt tccattgatt
     4141 ggcaaacgaa ttcgttaggt aattcattta aagaattagg aacaactttc ggtgcagcat
     4201 ttggttctga aggcatgcaa tctttattca gtgattcttc caagattcct caagtattga
     4261 tgacgatcat cgcattcagt ttatctgata ctttcgatac gatcggaaca tttatcggta
     4321 caggacgccg cacaggtatt ttctcaaaag aagacgagat cgcgttggaa gatggtcgtg
     4381 ggttcaaaac aaaaatggat aaagcactat ttgccgatgc gatcgctact tctgtggggg
     4441 ctatctttgg tacatcgaat acaacaactt atgtcgaatc tgcagctggg atcggggccg
     4501 gtggacgtac tggtttgact tcagtagtag tggcagtgct attcgcatta agtagtttat
     4561 tctctccact gatcgccatt gtaccaaatc aagcaactgc gccagcattg attttagttg
     4621 gagtaatgat gatggcttct tttgcagaca tcaaatggct agatatggaa gaagctttac
     4681 cggccttctt tgcatcgatc tttatgggat tgtgctatag catttcctat gggattgcag
     4741 caggatttat tttctataca attgtgaaag taatcaaagg aaaatacaat caagtttctg
     4801 ttgctttgtg gatcgttgat attttattca ttatcaactt catcattcta gcaacaattt
     4861 aactaaagag aagggattgg aacagtattt gttctgatct ctttttgttt tacataaaaa
     4921 gaacgttaca tataaaatga cctttgcggg atttttctct ttttactggc gagatcgttg
     4981 cttgtttatt cgaaaatagg tatgataaaa gagacgaaag agggatgata gatggagatt
     5041 aattggagaa aaaatttagt gattgcgtgg attggctgtt ttttcactgg tgcaagtatt
     5101 agtttagtta tgccattcat accagtttat gttgagcaac taggaacacc aaaagatcaa
     5161 atcgaattgt tttcgggact agcaatctct gtgactgctt ttgcatctgc cgtggttgca
     5221 ccaatctggg gaaatctagc tgatcgaaaa ggccgaaaac tcatgatgat ccgtgcagca
     5281 gcagggatga cattaacaat gggatctttg gcttttgtac caaacgcgta ttggcttttg
     5341 atcatgcggt tttggaatgg gattttgtct ggatatattc caaatgccac agcgatgatt
     5401 gcttcacagg cacctagaga aaagagcgga tgggcgttag gaacattgtc taccggagcg
     5461 attgctggaa atctgatcgg accttcaatg ggcggtgcac tggctcagtg gtttggtatg
     5521 gaaaatgtat tcttgatcac aggggctttg ttgatgatca caacggtatt gaccattttc
     5581 ttagtcaaag aagatttcca tccaatcgag aaaaaagatc tgataagcac caaagaaatt
     5641 ttttcaaaaa tggatcatct ctctattttg atcggtctct tcattacgac cttgatttta
     5701 cagataggaa tcactacgat cagccctatt ttgaccttat atattcgtga attgagcgga
     5761 agtacagaaa atatcctttt tgtcagcggc ttgattgttt cgatagctgg tgtttccgct
     5821 gtcttttctt caccgaaact aggaaaacta ggggataaga ttgggaatca aaaagttcta
     5881 attggaggac tcattttatc attttgctgt tatttaccta tggcattcgt cactacaccg
     5941 ctccaattag gtatacttcg ctttatttta ggcttttcaa ctggcgcttt gatgccttct
     6001 gtcaatacac tgatcagtaa gattacgcct caagaaggag tcagccgaat ctatagctac
     6061 aatcagatgt ttagcaactt tggacaagtt ttaggtccca tgctaggatc gacagtcgcg
     6121 catgcttata attacagtgc tgtttttatt gtgacaagtt tgtttgttct gtcaaatatt
     6181 cttctttctt tattcaactt tcgtaaagtt cttcgcagca gattataatg gtggtgagga
     6241 gtagaaaata aaaaagagct tgagaaatct caagctcttg gctgtttttt tacgaatggg
     6301 cagaggtttc ttttcttggc gcaactgcac caatcaaaag gataatgaca cccgcaatga
     6361 atgaaatgat tgttgtcaaa gtaaaatcat atgttccgct tgttaatgca gcaccaatat
     6421 aaccgactac ttgacctaga acgacagccc agaataatgt aacgatatat cgcataaaga
     6481 acacctcttt tctatcttaa ttttaccaga ttttctttga atgtaaagaa acgagaccaa
     6541 atctcaataa aatttgagtt cttgcgaaag aactcattcc tacagattac aagcaagcaa
     6601 atgaagtgga ataatcaact tcagcgcctg ctatcttaat gaaagtaaaa ggatcaaacc
     6661 ttttaacgct agtgaatttt aacgaaaaaa aaggcggaat gcaagatgtt tttcccacaa
     6721 ctttatacaa ttacttctta ttctctttta caaagtacga tacgtattcg agaatatgta
     6781 caagaagcaa aaaaacaagg ctacacttca ttagccataa cggataaaga tgtcttatac
     6841 ggtgcacctg aattctatcg tatttgtgta gaagaaggag tacagccaat cattggatta
     6901 catcttagtt atacttatga agaaaaaaat tatggcttgc ttgcgtacgc aaaagatgaa
     6961 ataggttatc agcagctgat gaaactttcc agccgaaaaa tgatccaaga aaaattggaa
     7021 cttgcagaaa tgacagaaac cgaccatcta gcgtttgttg ttccggcaaa tcattctgtt
     7081 tctgaggctt tcaataaaga ggataacttt ttcggtcatt ggcagttttt acttgaaaat
     7141 gtttttacta gaaattttta tatcggcttg acagaaaaca cgcctgatca attgcctgaa
     7201 tggtatcagt atgtgataaa agaaaattat tcgctttgtg cattacatcc catcgctagt
     7261 ttacaacaag aagaactttt tgcacttgaa gtgatgaaac atatcaaaga gggaagccaa
     7321 atacctttag aaaatttatc tgcatcttta ccactaacag caaatgtcta tcttctttca
     7381 gaacaagaaa tgatcaaacg attcgaaaaa gcgggtcttt cccaagctgt aataggcgcc
     7441 cagcgtttag ctgaatcgat ttcatttgaa ttccagttcc atcagaaatt acttccgcat
     7501 tatccagttc ctaatggaaa acaagctgga gaatatttaa gagagttatg ctattcgcaa
     7561 gtggaacgcc gaatcactga ctatacaaag gaataccaag aacgtttgga ctacgagtta
     7621 tctgtcatcc atcaaatggg attcgatgat tatttcttga tcgtatggga tgtaatggct
     7681 tttgctcata gaaacaaaat cgttaccggt gcaggtcgtg gttctgctgc gggttcatta
     7741 gtagcctatg tgttagaaat cacagatgtg gatccattag aatatgattt gctgttcgaa
     7801 cgctttttaa accccgaacg ctttacgatg ccagatatcg atttggatat tcctgacaat
     7861 cgacgtgaag aagtattgaa ttatgttcga gaaaagtatg gacaatatca tatggcacaa
     7921 atcgcaacat ttggcacaat ggcagcaaaa atggttctta gagatgcagc tagagtgttt
     7981 ggtttatcgc aaagtgaagc aaaccgctgg tcaaaagcaa tacccaatca attgaaaatc
     8041 acattgaccc aagcttataa aacttcgcat caattagtag aattagttca ggcaagcgaa
     8101 aaaaaccaac ttttattcaa aactgcagtg attttagaag gattgccaag acatgtttcg
     8161 actcacgcag caggtgtagt tatcagcgat aagaatctac ttgaactagt tccgctccaa
     8221 cgcggttctg atgaagcttt tctgacccag tatacgatga atgaggtaga agcaatcggt
     8281 cttttgaaaa tggacttttt aggactacgc aacttatcga ttattgattt tactttgaaa
     8341 gctgttaagc gagtagaagg gtatgagatc cgattaaaag agatcccgct aaatgatgca
     8401 aaaacgctgc tcttgtttca acgaggcgaa acctcaggag tatttcaatt tgaatctgct
     8461 ggtatccgga acgttttacg aagattagat ccagaaaaca tcgaagatat tgccgcagtc
     8521 aatgcattgt atcgaccagg ccctatgcaa aatatagata ccttcattgc caggaagaaa
     8581 ggaaaagaag ccattcgcta cccagatgat tcactgatcc cgattttgaa aaacacatat
     8641 ggagtgatcg tctatcagga acaaatcatg cagatcgcct cgactatggc tggatttaca
     8701 ttagggcagt cagatattct gcgacgggca gtgagtaaaa agaaaaaaga cgtattagat
     8761 gaagaaaagt cgcattttgt atcaggagca ttacaacagg gatattcgga aaaaacagca
     8821 gcgaccgtat atgaatatat cgaaaaattt gcaaactatg gattcaatcg gtcacatgca
     8881 tttgcttatt cattcgtggg attccaaatg gcgtatctaa aagcccatca tccaggtgct
     8941 ttctttacca gcctgatgaa ttctgttcgg cataacacat caaaattgaa agaatatatt
     9001 gcagaagcac gtaaaaataa actcaaactt tcctcgccgt ccattaatca aagtaattcc
     9061 agttttgagt tggtcaataa ggaagaaatc agattcggac tgacagcgat aaaaggtatt
     9121 cggcgtgatt ttgtagaaga tatattaaac gaaagaaaac aaaatggccc gttccagtca
     9181 gtggatcaat tcctcatacg gatcgataag cgttggctga aattagagtt actgcaatca
     9241 ctgacagcag taggagtttt tgatgaatta tctcctaaca ggaaacaatt gatgcttgat
     9301 ctcgaaggaa aaatccaaaa tatcgtttac agtggtggaa gtcttgactt gctgggaatc
     9361 atggcattaa aagaagaaga agtcgctgac tatacactag aagaacgctt gaagctggaa
     9421 gaggagtact taggtattta tttgtcaggt catcctgcag aagggtttga tcgtcttcgt
     9481 ttagctaaac aagttcagct gattacagat atcgtcccta atcaaccagt caatttatta
     9541 gtgatgatca aggaagttag agaaattcgg acaaaaaaag gagaactgat ggcctttgtt
     9601 gaaggaaccg acagtagtgg agaaatttct gtcacagttt ttccaactct ttatcgtaaa
     9661 tatcgtaatt tgtttgccgt tgatcgagtg atctttgttc aagggcggag cgaaatcagc
     9721 aaatataatc aggaactgca actaattgga gaaagctttg ctgatcctaa ccagttagaa
     9781 gcccagtatc cttcacaaac ttgctatttg cgtattacag aagaacggga agatcccgag
     9841 ataatgaaac aattacaagt gatttttcag aaacacccag gatatatacc agtagttatg
     9901 tatcacgaaa aaacgcaacg aaaagtagtt ctgtcagatg cttattgggt ggatgacagc
     9961 aagagtataa agcggcaact tatttatctg cttggcgaag ataatgtggt tttcaaataa
    10021 cttacggtaa aaaatactac ttgctttcat ttttttcatt tttttatccg taaatcaaga
    10081 caagtgagaa aaataatgtt agaatgttag tcgtggttaa cgcaaaaatg gttgtaaacc
    10141 taagtcatac ttaggataaa gtattttaat gaggtgaatg tttattatga aacgcatcgg
    10201 aattttgacc agtggcggag acgcacctgg aatgaatgca gctgttcgtg ccgttgttcg
    10261 taaaggtatt tacgaaggac tggaagtata cgggatcaat tatggttttg ccggtttagt
    10321 tgctggagat attcgtcgtc ttgatgtggc agatgtaggt gacaagatcc aacgtggtgg
    10381 tacattcttg tattctgcac gttatccaga atttgcaact gaagaaggtc aattaaaagg
    10441 aatcgaacaa ttgaaaaaat tcggaatcga aggtctagtg gttatcggag gagacggctc
    10501 ttatcatggc gcaatggctt tgacaaaacg cggtttccca gcagtaggta ttcctggaac
    10561 aatcgataat gatattcctg ggactgattt cacaatcggt tttgatacag caatcaatac
    10621 agtactagaa tcaattgacc gtattcgtga cacagcgact tctcatgttc gtacattcgt
    10681 tatcgaagta atgggacgca atgcaggaga catcgcttta tggtcaggtg tggctggcgg
    10741 agcggatgaa atcatcatcc cagaacatga ttttgatatg gcttcagttg ctaaaaaaat
    10801 ccaagaaggc cgcgatcgcg ggaagaaaca ttgcttgatc atcttggctg aaggtgtaat
    10861 gggcggaaat gaattcgctg agaaactttc agaatttggt gactatcata cacgtgtatc
    10921 tatcttggga cacgttgtcc gtggtggagc accaagcgca cgcgaccgcg tgttggcaag
    10981 taaattcggt gcttacgctg tcgaattgct tcaagaagga aaaggtggat tatgtgtcgg
    11041 tatccatgac aacgaaatcg ttgcgtctga catcattgat acacttgaaa acaacaaaca
    11101 taaaccagac ttatcactat atgatctaaa taattcactt tcattttaag agtgaaagaa
    11161 accaagctat ttaagctgtg tacagcttta aataaacaaa attgaatggg agagtttttt
    11221 aaatgaaaaa aaccaaaatc gttagtacat taggaccagc aagtaactca gtagaaatta
    11281 tttctcaatt gattgaatct ggggcaaatg tcttccgctt taatttttct catggtgacc
    11341 atgaagaaca attatcacgt atgactatgg tccgtgaagc agttaaaaag acaggaaaag
    11401 atgtcggtat tcttttagat acaaaaggag ctgaaatccg tacaactgtt caaggcacaa
    11461 cagaagctga ttttggtcgt gcaggataca tcaaatttga agtaggagat caaacacgca
    11521 tctctatgga tcctgaacat gtaggttcaa aagaaaaaat cgcggttact tacccaggat
    11581 tatttgacga tgttcacgtt ggcggacaca ttttatttga tgatggtttg atcgatatgc
    11641 aaatcattga aaaagacgaa gcaaaccgtg aattagtcgt agaagttaaa aatgcaggta
    11701 tgctaggatc acgtaaaggg gtaaatgcac caggcgtttc tattagtttg ccaggtatta
    11761 ctccaaaaga tgctgacgat attcgttttg gtttagacaa cgatatcgac tttatcgctg
    11821 caagttttgt acgtaaagca caagacgttc tagatatccg cgaaatctta gaagaaaaag
    11881 acatgactca tgttcaaatc ttccctaaaa tcgaatctca agaaggtatc gacaacattg
    11941 acgaaatcat caaagtttct gacggtgtta tgattgcccg cggagatatg ggtgttgaaa
    12001 tcccagctga actagtacca atggttcaaa aacgcattat caaaaaatgt aatgctgctg
    12061 gtatcccagt tatcactgct acacaaatgt tagaatcaat gcaagaaaac ccacgtccaa
    12121 cacgtgcgga agcttctgac gttgctaacg ctgtatttga tggcacagat gcaacaatgc
    12181 tatctggtga atctgcaaac ggggactacc cagttgaagc tgtttcaaca atggcacgta
    12241 tcgaccaaga agctgaaaat gcattatcag aattaggtac attccaattg aacgaattcg
    12301 ataaaacaga tgtaactgaa acaatcggtt tatctgtagc tcgtgctgca aaaaatctag
    12361 gcgtgaaaac aatcgttgct gcaactgaat caggatacac tgcaaaaatg atttcaaaat
    12421 atcgtccaga tgcagatatc ttggctgtta cttttgacga acgcacaaaa cgtggtttga
    12481 tgttaaactg gggcgtttac ccaacagttg ctgaaaaacc atcaacaact gatgaaatgt
    12541 ttgaactagc agctaaaaaa gctgttgaat taggtttcgc atcagaaggc gatttaatct
    12601 tgatcactgc tggtgtacca gttggtgaac gcggaacaac taacgtgatg aaaattcaaa
    12661 tgattggttc taaattatta gaagctcaag gcgttggaca aaaatctgtc gttgcaaatg
    12721 cagttattgc ttcatctgct gaagaagctg taaaaaaagc aaaagacgga atggttctag
    12781 ttgtaccatc aactgacaaa gaatatatgc ctgcaatcga aaaagcagct gctgttatcg
    12841 ttgaagaagg cggattgact tctcacgctg cagttgtagg aattgcacaa gatattcctg
    12901 taatcgttgg tgctaaaggc gcaacaacaa caattacaga aggtgaattg attaccgttg
    12961 attcacgtcg cggcatcgtt taccgtggcg aaacaacagc tatctaattt agcttaa
//
LOCUS       DACCQP010000078        12731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCQP010000078 DACCQP010000000
VERSION     DACCQP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.080"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..903
                     /locus_tag="IVO87_002277"
     CDS             <1..903
                     /locus_tag="IVO87_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450436.1"
                     /translation="TLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLL
                     ICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFS
                     TLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSN
                     EQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSK
                     GKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISL
                     FISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(1170..2339)
                     /locus_tag="IVO87_002278"
     CDS             complement(1170..2339)
                     /locus_tag="IVO87_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7450437.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2543..3718
                     /gene="mltG"
                     /locus_tag="IVO87_002279"
     CDS             2543..3718
                     /gene="mltG"
                     /locus_tag="IVO87_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7450438.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3854..4333
                     /gene="greA"
                     /locus_tag="IVO87_002280"
     CDS             3854..4333
                     /gene="greA"
                     /locus_tag="IVO87_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7450439.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4502..5233
                     /locus_tag="IVO87_002281"
     CDS             4502..5233
                     /locus_tag="IVO87_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7450440.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            5230..6297
                     /locus_tag="IVO87_002282"
     CDS             5230..6297
                     /locus_tag="IVO87_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7450441.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            6304..6936
                     /locus_tag="IVO87_002283"
     CDS             6304..6936
                     /locus_tag="IVO87_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450442.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            7076..7741
                     /locus_tag="IVO87_002284"
     CDS             7076..7741
                     /locus_tag="IVO87_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7450443.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7769..8062
                     /locus_tag="IVO87_002285"
     CDS             7769..8062
                     /locus_tag="IVO87_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7450444.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(8130..9077)
                     /locus_tag="IVO87_002286"
     CDS             complement(8130..9077)
                     /locus_tag="IVO87_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7450445.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(9255..10058)
                     /locus_tag="IVO87_002287"
     CDS             complement(9255..10058)
                     /locus_tag="IVO87_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7450446.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            10204..11118
                     /locus_tag="IVO87_002288"
     CDS             10204..11118
                     /locus_tag="IVO87_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7450447.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            11166..11636
                     /locus_tag="IVO87_002289"
     CDS             11166..11636
                     /locus_tag="IVO87_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7450448.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11721..12329
                     /locus_tag="IVO87_002290"
     CDS             11721..12329
                     /locus_tag="IVO87_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7450449.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12463..12663
                     /locus_tag="IVO87_002291"
     CDS             12463..12663
                     /locus_tag="IVO87_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7450450.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4303 a         1940 c         2503 g         3985 t
ORIGIN      
        1 acattgtatc aagatctgaa tagaccttat gtggatatat tacaaaaaag agcaatttat
       61 ctgggtaaag agtttttgat tggaagtata gattcagaat tactatcatt tggtattggc
      121 ttaactttgc ttatttgttt aattatacta acatttaact ttaaaaaatt aggaatctta
      181 tctaaaatca cttatataat gggtataata tctattctac tttgtacaaa aatatttcca
      241 tggtttgcct tacaaaatac tcctttaaat attattcaag ctccttggcg ttttatgata
      301 ttttcaacat tgttcatatc tttttcagta tctctattta tttctccaca gttggaaaaa
      361 ttttcttata caaggagaag aaattttata ctattattaa tattagctat ttcaatttta
      421 aattattcat cagtagttaa catggtgaaa gaaatgaatt ttcaaggaac ttattctaat
      481 gaacaaatgg aaaataaact tgaagaattt gagaattttg attatgtacc tataggctat
      541 aaaaaagatc aattgctttt gaaaaaccat caagttacag aagatggtca agttatttat
      601 agtagctttt ctccaaataa agatgaaatt acatttacgc ttaaatcttc aaaaggcaaa
      661 acaatagttt tacctgtatt ccggtataaa ggattaacgg ttactgttaa ttcaaaaaat
      721 gctacagtac taaaacaagg aggtcctttt gttacagtaa aaagtgaaag tggaataaat
      781 aaagtaacaa tcaggtattc atacacattt ttttctaaaa tctcattatt tatttctata
      841 atcacgctaa ttgttcttct tttttattgc ataaaattaa aaataagaac aagccatgaa
      901 tgatttgcta gcacatattt tagcaatcta aagatactat tttagattta ataggccctt
      961 tgtcaaataa tgttggtaaa gagaaatctt agaataattt ggacagaaca gaaagaagaa
     1021 cacctctttc tgttctgttt atttggaatt aaagtctgta gcaaaaattg atttttagat
     1081 ttgtcccaca ctctttccta cgtttagtcg agtacttttt ttaagcaaat gctattcaaa
     1141 gaagtagtat ttgctatctt ttcatcatat cagctatttg ccaacttagt agttccttcc
     1201 atcaggtcat agatttggtt tcttagatca gcagtaggca gtttgatagt tttgtcagct
     1261 tttatattgc tttccatgat gatagcatcc tgtgaatctt tgctaaatat agcagatgta
     1321 tcaaaagagt cgatatttcc ttgcgacagg atataattat ctccgctata taccccacca
     1381 ctatatggat gtccattcgc attttcacgc cataatgaga agagtaagtc tttggaaact
     1441 agttttccag ataaaacttg tgtaaacagc gtatttaaat catagacgga catactgata
     1501 ttaccagtcc ctaattcgtt atggatctct gtttcagatt ctgtcagtgg tttaaagtaa
     1561 tcatcctctt tagacatttg ataggatagt gcatgattgg atgaattttt gacttggtca
     1621 taaaattttg tatgctgtaa tcctaaaggt tcgatgatat catcggtcaa taatttttca
     1681 tacggttgat ttgttaattt gatcaaaatt ccagtcaata tggtaaagtt caatgaatca
     1741 taatgagagg tctgataatc tgtaaatttt aagtgctgaa tcgcaaattg aacgacttct
     1801 tcatcagtca tcggtatggt tggagaagca gttcggtgca agcctgagcg catatacagc
     1861 aagtcacgga tagtaatttg ctggctgcca gcaatttgtg gatagaattg agccagctta
     1921 gtatctaaag aaaggtgccc acgctcgact tgttttaaaa tcaaaagagt agtcaatgct
     1981 ttttgaaccg aaccaatctg ataataaaga gaaggatcat ttggttgttg cgtgagttta
     2041 ttcgcatagc catagccttt ttccatgatg atgcgattat ttttctttac gtaaattgag
     2101 ccaatataat cattttttct taatagttga tcgatttttt caacgagctg ttgttcattg
     2161 gtagtcagta tcaacttttt ttgagctgat tgagcatggg caactatctg agagtgttca
     2221 ttattgtaaa attgccatcc aagaatagtt cctgtagaga acgcactaag gctaaacaac
     2281 acgacaacga tcattaaaaa tgaatatttt ctttttttag catgttttcc ttttttcata
     2341 atagtttatt tagtctcctt tcttttttac agaaagaata aagctaaaat agcttgtatc
     2401 ttgataacat ttattttaat cttatagtaa tggatttact acttacattt ttgatagaat
     2461 atttaaataa aatcattcac gccaattcaa atatcatgta taatgaataa agttacgaac
     2521 ttgatagtaa ggaggagttg ttttgtcagg aaacaaacaa cacccaagta aagataaaaa
     2581 agaagccagt aaggcagcta cagcagatat aatcgacaag cggagaaatc tgcgtaaaaa
     2641 agaagataag attgtgagga agatcatttt agtgattgct cttacattat tgattattgg
     2701 cggatttctt ggatttaccg tgtatcgtta tgtagatagc gggttgaagc ctttagataa
     2761 atcagatgac caattagtcc aagtcgagat cccgagtggc tcttctaaca agcagattgg
     2821 tgagattttg gagaaggaca atattattaa aagcgggatt gttttcaatt attatacaaa
     2881 atttaaaaat ttaactggct ttcaagctgg ctattatcag ttagcgccta atatgactct
     2941 tgacgagatt ggcaaacagt tgcaagaagg cggtacttct gagccaacaa aagttgcaga
     3001 tgggaaaatt gccattccag aagggtacga tatcgatcag attgctgaac gggtagcgaa
     3061 ggtaactggt aaagacaaga aagaattttt agatttagtg aatgatgaga ctttctttaa
     3121 cgagttgcac caaaaattcc cagaactgtt ggaaagtgca tctaaagctg agaacacaaa
     3181 ataccgtttg gaaggttact tattcccagc cacatatgat tattacacga gtacttcgct
     3241 taaggatcta gtgattgaaa tggtgaacaa gacgaatacg gtcatgcaaa actattattc
     3301 agctatcaag cagaaaaatt tgactgtaca agaagtttta actctagctt ctctagttga
     3361 aaaagaaggc gtaaaagaaa atgatcgtaa aaacattgca caagtcttct ttaaccgaat
     3421 caaagcaaat atgccacttc agtcagatat ctctgtttta tacgcattag gtgaacacaa
     3481 agaattagtg acgtatgaag atacagctgt agattcacca tataatttgt ataccaatac
     3541 cggctatgga cctggacctt ttgacaatcc aagcgaagag gcaatcaagg cagtattaga
     3601 accagcagaa aatgactatt attatttcgt agcagatacg agcactggaa atgtttattt
     3661 cgctaaaact tatgaagaac atatggaatt agtggaaaaa tatgtaaata attcttagaa
     3721 aaacaggctc tagacattga tcatttttag aaaaacattt acatttgaag aaaagcgtgg
     3781 taatcttttt tggactatat tcctcaaaag attacctttt gcggtatttt atcgaaaaaa
     3841 ggagacgtta cacatggtag agaaagttta tccaatgact ctagaaggta aagaaaaatt
     3901 agaaaaagaa ttagaagaac taaaaacagt caaacgcgga gaaatcattg aacgaatcaa
     3961 aattgcccgc ggtttcggag atctttccga aaactctgaa tatgaatcag cgaaagatga
     4021 acaagcattt gttgaaggaa gaatcacaac aattgaaaat atgattcgtt ttgctcaaat
     4081 catcgataac gacggtgtag atgcagatga ggtatcaatt ggtaaaacag ttacattcat
     4141 cgaactacca gatggagaag aagaagagta cacgattgtc ggaagcgctg aagcagatcc
     4201 gttttctggc aaaatttcca atgattctcc tattgcgcaa gcattgattg gcaaacgtaa
     4261 aaatgatgaa gtaactattg ctactccagg tggcgatatg ttagttaaaa tcgtcaaagt
     4321 agaacaagcg tagtctgact gaaagatcta gagagttcat cttactgatg atacactcta
     4381 gatctttttc tctttgtgta ttttagattt caacaaacga tcttacatct aaagtcctat
     4441 agccaacagt taaaaaaata ggtatgatga aatgagaatg tgtcaggagg gagaaagagc
     4501 gatgaatagt tcttggcgtt tttttgtggt agttgaagca ctactgctgt tatttgctgt
     4561 ttggcaaatc gtaaacaata ctggactttt gcttttagtg atattcggta ttttcaatat
     4621 atatctagct atgcgcaaac atcctcgtac taaatttcaa aattttcaat tgatattagg
     4681 aagtttggtc atttttttta gcttagtcaa cagtcctgct ttatggatga tggctgtatt
     4741 tgccgtttta tttattggtc tgaaaggtgt agagatttca gggatagatt taaccaaaaa
     4801 tgctttttgg cgaaagaaac agatcatgat gatccaaaca gagcaattaa aaacacataa
     4861 taatgagcgg aaaagacagc aattattcgg taaccaacgt attggaaacg acgtctatga
     4921 atgggacgat atcaacatcg ccgtcatttc tggtgatact atcatagatt tgggcaatac
     4981 gcttttgcca aaagatgata atattgtgat tgtaagaaaa gggataggca gaacacggat
     5041 cttagtacct ttgggcgtag ccatccgatt agagcatgcg acattagtag gaaatgtctt
     5101 gtttgaagaa gagcaatttt ctttgaaaaa tgaacaaatc aaaatctaca gcagtgatta
     5161 tgatgaaaac cctcgtagat tgaagattat tactaatacc ttgattggag atgtcgaggt
     5221 gatacgaata tgatgggaaa aatatccaga gcgatgctag ctgtttattc ggggattgcg
     5281 gctttcctta ttattctatt ttcacttttc acttattttt atgccagcaa tcaaagtcat
     5341 tggtggggag aattactgcg tgcacgttta ttatatgttc cgcttatctt ccatttgtta
     5401 gccatttcct taggtgtagg attaattgtc tttctattat tatcacttat tcaaaagaca
     5461 aaatacggga aaatcgaaga aaagctgcgt gcactttctt ctggcaatta tgaatccaaa
     5521 ttgttgcttc ttccgatccc aagtgcatcg gacgatttat acatcaaaga tattgataag
     5581 gaaatcacta agataaaaga aaaaatgatt gaagtatcta gtgaattaca aattgtaacg
     5641 agccgtcccc aatatgtaga tggacaaacc aaagaagaaa ttttagaatt agaaagacat
     5701 cgattagccc gtgagctgca tgattcagtt tcgcaacaat tgtttgcagc aatgatgatg
     5761 atgtcggcat tgacggagca agcagaaaaa agcgagacac cagaaatgtt tcgcaagcag
     5821 ttgaaaatgg tagcagagat catcaacgct tcccagtctg agatgcgtgc gctgcttctt
     5881 catctacgtc cagtcaattt agaagagaaa agtttaaagc aaggtatcga gcagttattg
     5941 aaggaattgc aaaataaaat ccagatttcg ctgaaatggg atgtagaaga tgtaaaacta
     6001 tctagttcca ttgaggatca cttgttccga atcgttcaag aattgttatc aaatacatta
     6061 agacatgcta aagccaatga attagaggta tatttgcaca aaatagacaa taatcttttg
     6121 ttacgtatta ttgacgacgg aacagggttt aatatgaatg aaacgaaaac gggaagttat
     6181 ggattgaaca atatcaaaga acgagtagct ggtattggtg gaacagtaaa aatcattagt
     6241 tttaaaggtc aaggtacaag tgtagaaatc aaggtccctt tgatgaagga ggcatagata
     6301 aggatgataa aagttttatt agtagatgac catgaaatgg tgcgcttagg cgtctcttct
     6361 tatttatcta ttcaagaaga cattgaagta attggagaag ctgaaaatgg acgacaaggc
     6421 tatgagaagg cgatgacact tcgaccggac gtcattttaa tggacttagt catggaagaa
     6481 atggatggta tcgaatcaac aaaagcaatc ttaaaagatt ggccagaagc taaaatcatt
     6541 atcgtaacca gttttattga tgacgaaaaa gtttatcctg ccattgaagc aggtgcagca
     6601 gggtatttgt tgaagacctc taccgcacat gaaattgctg atgctataag ggcaacgcaa
     6661 cgtggcgagc gagtattaga accagaagtg acaacaaaaa tgatggaaaa aatgagtcgg
     6721 cgaaatgagc ctgtattaca tgaagaacta acgaatcggg aaaatgaaat tttaatgctg
     6781 atttctgaag gaaaaagtaa tcaggaaata gcggacgaat tgtttattac actgaaaaca
     6841 gtaaaaacac atgtttccaa tatattggct aagctagaag tggaagaccg tacacaagcc
     6901 gctatctatg cattcaaaca tggtttggtg aaataaaaac gatgataaag aggataatca
     6961 aaagctgaga aaataactat tatttttctt ggctttttta caataatgat gcttgctttt
     7021 gtttttgtaa aggctttgtt atactgagaa aagaagatac taaaggagga aaacggtgaa
     7081 acaaaatttt gcaattgtag gtcttggacg atttggcgga agtatctgcc gaacactgat
     7141 cgaagctggt caagaagtac tggctattga tagtaatgaa gatcgggtca atgaatatat
     7201 gaatatagcc actcatgctg ttgtggcaaa tgcacaggat gaaatgacac ttcgatcatt
     7261 aggcataaga aatttcgatc atgtaattgt cgctattgga gaagatattc aagccagtat
     7321 cttagttact ctgatggtaa aggaaatggg cgtaccaaat attttagcaa aagcccaaaa
     7381 tgagtatcat gccagagtac ttgaaaagat cggagctgat cgcgtcgtcc atcccgagcg
     7441 agacatggga ataaggattg cgcataattt agtgtcaaag aatattttag attacctaga
     7501 gttgtcagat aaatattctt tagcagaaat ccgagtctcc aatcctaaat ttttcaacaa
     7561 aacattagct gaattaaatt tcaggcaacg ttttgactta acagtagtcg ccatacgaag
     7621 agtagatcaa acggttatcg cttctcctgc agcagatgaa tatgttcgtg aaaatgataa
     7681 tctactggtt attggagaga caggcgatgt ggatattcta gacgataaga tgaatcaata
     7741 gaaagaaaag agaaagtagg ttgttgggat gaaattaacg gtaacgccaa aagcacaaaa
     7801 gtggtttgac tcagaaattg atctgccaga aacttacgga atacgttttt ttggaaaagt
     7861 ctacggcaaa acagaggttc atgatggttt ttcgattgga atgtcagttg aacagccaga
     7921 acatcctgta aagaaagtgg aaattgatgg gacacttttt tttatcgagg aagcagatga
     7981 atggttcttt aaaggatatg atttacttgt tgattatgat cccaacctgg aggagccaac
     8041 ctatcacttt aaaaaacaat aaaaaaagag agggtgacac aagttttgtc accttctctt
     8101 ttttcttttg aaaatattgt tttcagctat taatatcctg cagacaattt tacttgattg
     8161 gtttgaagct catgggtggc aaagtagctt ttcagattgg ttaaaaaaat agccattaat
     8221 ttcttttgaa aatcaggtgt caaacctgca atgtgggaag taatcagtac attgtccatt
     8281 ttccaaagag gatgatcttg tggaagaggc tcttcctcaa aaacgtctaa tgctgcaaag
     8341 gaaagttgct ggttatttaa agcctgcacc agatcttgtg tcttaacaga agcaccacgg
     8401 ccaacattga tgaatacaga ttttggatca aagtgttcaa acatatgctg atcaaagagc
     8461 ccagttgttt cctccgttag aggtaagata ttgactacga aatctacttc aggaagaact
     8521 tcctttagtt gatttaaaga aaaagtcttt tccattcctt ctgctggatg accggatgta
     8581 ttgattccat agcagttcat gtctaatgct ttgcattttt ttgcaatcat ttttccaata
     8641 tggccagttc caatgattag tatttttttc ccttggatag attggtaatg gaccgatgaa
     8701 cctaaccatt tcatgtcact ctgtgccttt tgtgcttgaa aaagaccacg agaataccca
     8761 aggatgattc ctaagacatg ctccgtaatc gattcactgt ggatgccact accatttgaa
     8821 agtaaaatat gtttctttga aaaagtctca aggggtagct gatcaacacc agctgagata
     8881 gattggaccc atttcaaggt agaagaggct aatagttttt cttggtagtc tgagttccag
     8941 cccaaactga tctctacatt ttgtagatcc tctggttgta attctgtttt aaagatataa
     9001 ccaggcgcta tagtatggat ttcttcgata aattcatcac gaaatggacg atctgcataa
     9061 atgattcttt ctgacatagt taatttcccg cctttcttta ctctaagtgt agcgcataca
     9121 agaagaactt tgaagcgcct tgcttgtttt ttgagaaaga aatatttttc aaataaaaaa
     9181 ggggctgtga caaaaaaatg tcacaacctc taaaagggtc tattgaagta gaaataacga
     9241 gttgtcttta tttcttaatt gatcgatatt tttgcccatc ctttgacagc ttgagcaaaa
     9301 ataaagattc ctgcaaaagc aatgaaggcg ctagtgtcaa aagaaggaag aaagaccatc
     9361 aagattgttg caattatagt aggcaatgaa attgaataga tcaatgtttt taaacaatca
     9421 agaaaagtag cttttgttag tcgaaagcgt gcatagagaa aagcagcaat gttggtaaat
     9481 agcaaagtga tcagcagatt cagaaaacta ggataaatag aaaatataaa tgtcaggact
     9541 tttatccaaa gtggtataga tgcttcactt aaaaaagaac ggagctgctt acctgttaag
     9601 ttttttaaag attcattggt gtagggaagt tctaattggt tattgttcaa caaagcagaa
     9661 gtcgtgcctg tattaggaag agctatgata agtttgtttt ttaacatccc gacactaaca
     9721 aaattcccca tcaaatcagt agagatgtcc tgttctgttc gttttccctc tggatcaaat
     9781 gtgaagataa tggagtttgt ttggtaaatg aatccttctt tttcttcggt ttgaagctgc
     9841 ccgttttgga ttgtaaaatc aggaatgcga gtagcaatct tttgcccatc caattgaatg
     9901 ttgtttaaga cttgaaaaac ttgaaaagag attggcaaag ccataattat actcaaaact
     9961 aaaagataga caatggcttt ccaaaatggg acatttctcg cctcctttag gtctttgaat
    10021 cgtataaagg aactgataat taattgtttt gttgtcataa gtactcctca tctagtagtg
    10081 tcatcacttg caattctagc gaagtatcaa gtgaaataca agtgctttaa ggaaacagct
    10141 gaatgattgg tgaaatatca aaaaaatgtt attcttgtct aaagactatc caatataaat
    10201 aaaatggagg aaggaatagt gcctacacag ccacagagat catcaaaaaa actatttgga
    10261 gtagtggttt tgaatgcggt gatcacttta tcagaattta ttggagggat tatgtcaggg
    10321 agtttgtctc ttgtttcaga tgctttccat aaccttagtg atacattggc aattattttt
    10381 tcttacgggg cacaaaaaat ggcccgaaaa gaagcaaata aaaaacgtac gtatggctac
    10441 cagcgtttag aaatattgag tgctttcatc aactcattca tattgatcat attatccttg
    10501 tttttaaccg ttgaagcatt caagcggttc acttcaccag aaaagataaa tagtcatttg
    10561 atgctaacag tagcagttat tggattgctc gcgaatttgt tctcagtatt attattacga
    10621 caagaagcag atgaaagttt gaatataaaa tctagttatt tgcatttgtt aagtgataca
    10681 ctgtcttcgg taagtgtgat tatcggagca attttgatcc gttttttcga tatctactgg
    10741 atcgatccag ttattacact gatcatttcg atatatatat tgatagaagc gataatggtg
    10801 atcaaaaaag ctgccgctat tttgatacaa agtgcacctg ctatcgacta tgaaaaaatg
    10861 gaacaagaaa ttgaagcaat cgatggggta aaggatgttc atcatgtcca tatttggcag
    10921 tatagcgaaa agataattat tttcgacgga catattgatt ttgaagatca gctactaagc
    10981 gagattgaaa agatatatcc tcgtataatt tctttattaa aattaaaata tggtattaca
    11041 catgtgacga tccaagcaga gacaaaggca aaggatcaaa aaaaattaat ttttttaaat
    11101 aaagatgaaa gttattgaaa tatagagtat gataaataag aatttttaat aaggtaggtt
    11161 ggctcatgaa gcagtatcga cagttaaaag gatatttaga agcaaaagga tattgggaga
    11221 tactagtcta tctctttttt ggtggtttgg ctacgattgt aaattttgtt agttttgctc
    11281 tcgcacgcca atattttgat ttaagtatgg ccttatccaa ttctatttcg tggttttgtt
    11341 ctgtattgtt tgcttttgta acgaacaaac tttgggtatt ccattcaaaa tcaccgaatt
    11401 ttacacatgc acttattgaa tgcggtaagt tcttcttcta ccgtatcctt tcttacggat
    11461 tggacatggg ggcaatggta ttgttgatca atgttatgaa cagcaatgag tatgtggcga
    11521 aaatcatcac tcaaattatt gttatccttg cgaactatat tttcagcaag ttgtttattt
    11581 ttaaagaaac tgaggttttt gaagaagaag taggtcaaag tgaaaaagac gactaggcat
    11641 aacaattgaa gaatttcctt ctctttgtta ctctttgtaa tgtagacatt attaagaaaa
    11701 cattgaggag gaattttttt atgacttaca cattaccaga tctaccttat gcatatgacg
    11761 cattggaacc ttatattgac gaagaaacga tgcatctgca tcatgataag catcacaata
    11821 cttatgtgac gaatttaaat tcagcaattg agaaataccc agaattaggc gaaaaaacaa
    11881 tagaagaatt attatctgat atggacgcta ttccaacaga tatcaagaca gctgtacgta
    11941 acaatggtgg cggacatgct aaccattcat ttttctggga aatcatggca ccaaatgctg
    12001 gtggcgaacc tacaggagaa ataaaagaag cgattaatga agcttttggt gatttttctt
    12061 cttttaaaga agaattcaaa aaagcagccg ctggacgatt tggttctgga tgggcttggc
    12121 ttgtaatgga aaatggaaaa ttagctatta cctctactgc aaatcaagat tctccattga
    12181 tggaaggaaa gacaccaatt ctaggtttgg acgtttggga acatgcttat tatttgaaat
    12241 ataaaaatgt ccgaccagat tatattgcag cattctggaa tgttattaat tgggatgaag
    12301 taaacaaacg tttcgaagca gcaaaataac catttcctcc gtcatatcag tcttaaaaat
    12361 aatttggaaa ataaaagagc gaaagcttgc tagcgttttc taaattgggt ataatgttct
    12421 tgcaaggtta cttgcaagaa cttttttgga ggatatacat ttatggaaca cggaacggtt
    12481 aaatggttca acaatgaaaa aggatttggg tttattacag tagacggcgg agatgacgta
    12541 tttgttcatt tctcagcaat tcaaggagac ggcttcaaga gcctagaaga aggtcaagaa
    12601 gtagaattca cgattgttga aggtgcacgc ggacctcaag ctgctgaagt gactaaatta
    12661 taataatttg ttgaataaag acacgacttc ttagagagtc gtgtcttttt gtctatttat
    12721 gaagttttta g
//
LOCUS       DACCQP010000079        12582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCQP010000079 DACCQP010000000
VERSION     DACCQP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.081"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IVO87_002292"
     CDS             <1..537
                     /locus_tag="IVO87_002292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450451.1"
                     /translation="DMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IVO87_002293"
     CDS             564..2375
                     /locus_tag="IVO87_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7450452.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3905
                     /locus_tag="IVO87_002294"
     CDS             2436..3905
                     /locus_tag="IVO87_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP7450453.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3916..5925
                     /gene="topB"
                     /locus_tag="IVO87_002295"
     CDS             3916..5925
                     /gene="topB"
                     /locus_tag="IVO87_002295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7450454.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6271..6867
                     /locus_tag="IVO87_002296"
     CDS             6271..6867
                     /locus_tag="IVO87_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7450455.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6880..7779
                     /locus_tag="IVO87_002297"
     CDS             6880..7779
                     /locus_tag="IVO87_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7450456.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7782..7913
                     /gene="ssb"
                     /locus_tag="IVO87_002298"
     CDS             7782..7913
                     /gene="ssb"
                     /locus_tag="IVO87_002298"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7450457.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7935..8267
                     /gene="ssb"
                     /locus_tag="IVO87_002299"
     CDS             7935..8267
                     /gene="ssb"
                     /locus_tag="IVO87_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7450458.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8289..8546
                     /locus_tag="IVO87_002300"
     CDS             8289..8546
                     /locus_tag="IVO87_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450459.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8705..8827)
                     /locus_tag="IVO87_002301"
     CDS             complement(8705..8827)
                     /locus_tag="IVO87_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7450460.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9017..9241
                     /locus_tag="IVO87_002302"
     CDS             9017..9241
                     /locus_tag="IVO87_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450461.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            9684..9890
                     /locus_tag="IVO87_002303"
     CDS             9684..9890
                     /locus_tag="IVO87_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7450462.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9890..10141
                     /locus_tag="IVO87_002304"
     CDS             9890..10141
                     /locus_tag="IVO87_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450463.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10322..10594
                     /locus_tag="IVO87_002305"
     CDS             10322..10594
                     /locus_tag="IVO87_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450464.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11039..11218
                     /locus_tag="IVO87_002306"
     CDS             11039..11218
                     /locus_tag="IVO87_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450465.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11277..11633
                     /locus_tag="IVO87_002307"
     CDS             11277..11633
                     /locus_tag="IVO87_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450466.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4639 a         2066 c         2617 g         3260 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc
     2941 atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc
     3001 aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca
     3061 agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat
     3121 tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag
     3181 atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag
     3241 agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg
     3301 acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc
     3361 cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta
     3421 aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa
     3481 ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc
     3541 atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg
     3601 atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg
     3661 gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag
     3721 gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     3781 aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac
     3841 aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     3901 agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     3961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     4021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     4081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
     4141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
     4201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
     4261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
     4321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
     4381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
     4441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     4501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
     4561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
     4681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
     4741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
     4801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
     4921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
     4981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
     5041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
     5101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     5161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     5221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     5281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     5341 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     5401 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     5461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     5521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     5581 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     5641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     5701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     5761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     5821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     5881 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     5941 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     6001 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     6061 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     6121 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     6181 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     6241 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     6301 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     6361 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     6421 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     6481 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     6541 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     6601 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     6661 tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat ttggaatccc
     6721 tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat ggagtcggac
     6781 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga caaaacgatg
     6841 cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac gcagttaaaa
     6901 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     6961 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     7021 ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac aaccaaagat
     7081 attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac ggttctaaac
     7141 gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     7201 acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca tttggaaaac
     7261 atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc tgcaacgatc
     7321 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     7381 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     7441 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     7501 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     7561 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     7621 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     7681 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     7741 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     7801 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     7861 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     7921 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     7981 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     8041 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     8101 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     8161 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     8221 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     8281 gagtaagcat ggcaaaaaaa attagtctga cgatggatct attgataccc gtaattgcat
     8341 ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga attgcgaaag
     8401 gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa agagcaggaa
     8461 aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct gttatactta
     8521 cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt cttgcgaaat
     8581 attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt aacagcaccg
     8641 attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct gctaaagtag
     8701 acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc aaagcaatgg
     8761 taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa aggcttactc
     8821 ctttcataga ttttagcact tacattcata agcaccacca cctttccaga agtcgccacc
     8881 gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata aaaattctca
     8941 attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa tatattggtt
     9001 tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta cattacgcac
     9061 gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata aattgctgat
     9121 tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac gaaagcaact
     9181 agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgcgatcga tgagcatata
     9241 aaagaagagc tgcagtccta aatgaggatt gtagctcttt ttgttttaca tagaaacagt
     9301 aaaaatgaaa aggcctgttt aggaaggcac aagcgccttc tcaattgaaa gacctttctc
     9361 tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctacgc gttcactaag
     9421 ataaagtgtc ttgcatagca agacaaaata gaaaaaattt cctctcacca aaaggttcgc
     9481 tcctgttcgt tatctcttta ttgatttgtc agaccggcaa caagttgcct tttatcacca
     9541 aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtcttgt cgcttttcgt
     9601 tgattcattc tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt
     9661 cctaagagga ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca
     9721 gcgaattatt cccaaactat catgcaccac atactgtcga atgcgattgt ggagaaagag
     9781 cgaaatgtat caaaagctat tgtttgtatc aatgtccaac atgcgggaaa aaatatacct
     9841 tgtcttatgg cgattatatt ttaattgatg agaaaggaaa gaaacgctga tgaaaacaca
     9901 acacaaatta ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc
     9961 tgaaaaagta gcagaagttt tacttattcc gcggtactgg acgatagaaa cccctttagt
    10021 agaggatgca ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca
    10081 ttatcaagaa aacttggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta
    10141 aaaaggagaa aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga
    10201 taaaatggca caaaaaatgg aggatatgac gttcctatac aacgaaacac gtgttccaaa
    10261 aaaacattat aaagaaaaat tgtcggtagc tgtggaagaa atgattgaat aaggtgtcga
    10321 aatgaatctt attgcgacgt actatcgaac actggaagaa ctgaaaaagc aaaatgcaaa
    10381 atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc
    10441 ttctgaatac caagctttag aactaacgta tgcgaaattt atcgaaacga aaaaagccaa
    10501 gactgtttca agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcatatta
    10561 caccatgaaa aaggaagaca acgaaaatga ataaggtgat ttatcaacta acaacggatt
    10621 ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa aagtgagaat
    10681 gtgtgaaaca aggacaaaaa aagcctagta actaaggaaa tggttcttat ttcgcaccaa
    10741 tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa acgggcaaag cccgataact
    10801 taagagaaag aaggtgatcc atttgaacaa gattattccc ttattgttag tactgtttgt
    10861 tggcgaaata cttatgcgag atagtgcagc tgtacagtta aacctcgctg tatttttggt
    10921 aatactggtg ttttgtttga tcttttggcg gtttgtatta gtcctcgtga gtctactatt
    10981 caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggagt gagtaccaat
    11041 gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga gacaaaaaga
    11101 attgcagcaa ctaaagcaga agtatggcaa aagtgaatct aagattatgt gtattgcgtt
    11161 agatctattg atcgcgcaag aaaaagcagg atttgatgta ccagcgctaa aaaagtgacg
    11221 tcacttttta atttgatcta tataagtgat atcactttta ccaaggaggg aattaaatgt
    11281 taaaaactaa aaagaattcg tacaaattaa agcttagtat actatctatt atttgtcttt
    11341 taggcatgat aattagtggt catttgtata gagtggcata tacgcagttt attgagtaca
    11401 aggttcactc tttaggatta gctggggatc gatacgttga atatgtccat tattcattaa
    11461 aatgttcttc atatattctt ctttggctta ttttttcatt gtttgaattg ctaaatttaa
    11521 tagcaatatc ttcatatcac ttagataagc taaataccaa atctagccgt tataacgaag
    11581 tgtttaagga aaatgtgaag ttagaaagtg acttaaacaa gctaaaaaaa taaaaagaag
    11641 aaaacataag ttaactaaaa ttattttatt aagaatgatg gaatataagg ataaggttac
    11701 caacataacg atcattactt ctctgatatg ctatggactt ctatcaatca gtattttgtc
    11761 tatagattat ggagtaacta tactgcaagc cttagttttg gctatgccta tgtatctatc
    11821 tttgtttatc atgcatgaaa tatataagct atttgtttta tcaaaaaatg tagaaatacg
    11881 ggtaaatatt ttggtgaata gttatcaaaa agaactcaaa gaaagaagac ggtggcggta
    11941 tttattgaaa ggatgaaaaa gtaggatttg acatactggt attcagaaag tgccgtcact
    12001 tttaaaacaa tggaaaaggt ggagataaga tgaagaaaaa acaaactaaa ctatcattag
    12061 cagatattta acacagttaa ccgcaactga agatggtgtt gttgaatttg aacaatctga
    12121 aattgcggta gtagatgatc gttaccttaa aatgccttac ttctttgacc aagcaaaggt
    12181 catttgcctt tttcactacg atggggtacg tgattttagg cgttaaaaag tagtgattat
    12241 atcaacttta cgagtaaata taaatttaga catgaaactg aattatccaa tggaaaatca
    12301 tggtttataa tctagaaaaa ttatagtaat gattggatta tatatattct ttcgtatttt
    12361 cgtacaactg tagcttgtca aaattgagaa atttaaccga ttaggtactt tacatcgtta
    12421 tttgggtaat ttctaaaaca tgaagcttac caacgaataa actgagacta tcttattaat
    12481 tgatcaatat aagaaaatat ctttgaggag atgatgtact atgttttcgt ctatccacga
    12541 ttggttaggt ggtcttttcg gcggtggcgg taaaggtttt ta
//
LOCUS       DACCQP010000080        12285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCQP010000080 DACCQP010000000
VERSION     DACCQP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.082"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IVO87_002308"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IVO87_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7450467.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IVO87_002309"
     CDS             complement(738..1460)
                     /locus_tag="IVO87_002309"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7450468.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IVO87_002310"
     CDS             complement(1596..2477)
                     /locus_tag="IVO87_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7450469.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVO87_002311"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IVO87_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7450470.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IVO87_002312"
     CDS             3717..4196
                     /locus_tag="IVO87_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7450471.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IVO87_002313"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IVO87_002313"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7450472.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IVO87_002314"
     CDS             5497..5625
                     /locus_tag="IVO87_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7450473.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IVO87_002315"
     CDS             5629..6174
                     /locus_tag="IVO87_002315"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7450474.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IVO87_002316"
     CDS             complement(6628..8319)
                     /locus_tag="IVO87_002316"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7450475.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IVO87_002317"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IVO87_002317"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7450476.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IVO87_002318"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IVO87_002318"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7450477.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IVO87_002319"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IVO87_002319"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7450478.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3370 a         2540 c         2209 g         4166 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagc
//
LOCUS       DACCQP010000081        12009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCQP010000081 DACCQP010000000
VERSION     DACCQP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.084"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(113..475)
                     /locus_tag="IVO87_002320"
     CDS             complement(113..475)
                     /locus_tag="IVO87_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7450479.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(486..1607)
                     /locus_tag="IVO87_002321"
     CDS             complement(486..1607)
                     /locus_tag="IVO87_002321"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7450480.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1623..1973)
                     /locus_tag="IVO87_002322"
     CDS             complement(1623..1973)
                     /locus_tag="IVO87_002322"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7450481.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2103..3614)
                     /locus_tag="IVO87_002323"
     CDS             complement(2103..3614)
                     /locus_tag="IVO87_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7450482.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3936..5288)
                     /locus_tag="IVO87_002324"
     CDS             complement(3936..5288)
                     /locus_tag="IVO87_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7450483.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5441..6517)
                     /locus_tag="IVO87_002325"
     CDS             complement(5441..6517)
                     /locus_tag="IVO87_002325"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7450484.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6713..8458
                     /locus_tag="IVO87_002326"
     CDS             6713..8458
                     /locus_tag="IVO87_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7450485.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8738..9175)
                     /locus_tag="IVO87_002327"
     CDS             complement(8738..9175)
                     /locus_tag="IVO87_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7450486.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9159..10532)
                     /locus_tag="IVO87_002328"
     CDS             complement(9159..10532)
                     /locus_tag="IVO87_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450487.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10854..11342)
                     /locus_tag="IVO87_002329"
     CDS             complement(10854..11342)
                     /locus_tag="IVO87_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450488.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11364..11966)
                     /locus_tag="IVO87_002330"
     CDS             complement(11364..11966)
                     /locus_tag="IVO87_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7450489.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3445 a         2612 c         2168 g         3784 t
ORIGIN      
        1 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
       61 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
      121 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
      181 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
      241 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
      301 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
      361 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
      421 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
      481 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
      541 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
      601 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
      661 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
      721 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
      781 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
      841 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
      901 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
      961 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
     1021 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
     1081 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
     1141 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
     1201 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
     1261 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
     1321 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
     1381 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
     1441 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
     1501 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
     1561 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
     1621 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
     1681 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
     1741 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
     1801 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
     1861 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
     1921 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
     1981 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
     2041 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
     2101 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
     2161 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
     2221 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
     2281 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
     2341 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
     2401 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
     2461 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
     2521 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
     2581 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
     2641 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
     2701 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
     2761 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
     2821 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
     2881 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
     2941 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
     3001 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
     3061 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
     3121 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
     3181 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
     3241 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
     3301 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
     3361 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
     3421 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
     3481 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
     3541 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
     3601 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
     3661 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
     3721 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
     3781 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
     3841 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
     3901 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
     3961 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
     4021 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
     4081 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
     4141 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
     4201 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
     4261 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
     4321 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
     4381 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
     4441 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
     4501 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
     4561 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
     4621 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
     4681 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
     4741 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
     4801 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
     4861 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
     4921 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
     4981 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
     5041 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
     5101 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
     5161 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
     5221 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
     5281 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
     5341 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
     5401 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
     5461 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
     5521 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
     5581 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
     5641 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
     5701 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
     5761 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
     5821 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
     5881 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
     5941 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
     6001 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
     6061 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
     6121 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
     6181 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
     6241 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
     6301 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
     6361 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
     6421 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
     6481 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
     6541 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
     6601 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
     6661 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
     6721 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
     6781 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
     6841 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
     6901 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
     6961 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
     7021 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
     7081 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
     7141 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
     7201 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
     7261 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
     7321 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
     7381 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
     7441 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
     7501 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
     7561 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
     7621 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
     7681 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
     7741 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
     7801 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
     7861 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
     7921 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
     7981 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
     8041 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
     8101 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
     8161 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
     8221 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
     8281 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
     8341 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
     8401 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
     8461 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
     8521 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
     8581 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
     8641 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
     8701 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
     8761 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
     8821 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
     8881 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
     8941 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
     9001 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
     9061 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
     9121 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
     9181 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
     9241 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
     9301 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
     9361 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
     9421 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
     9481 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
     9541 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
     9601 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
     9661 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
     9721 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
     9781 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
     9841 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
     9901 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
     9961 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    10021 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    10081 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    10141 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    10201 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    10261 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    10321 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    10381 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    10441 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    10501 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    10561 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    10621 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    10681 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    10741 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    10801 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    10861 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    10921 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    10981 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    11041 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    11101 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    11161 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    11221 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    11281 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    11341 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    11401 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    11461 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    11521 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    11581 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    11641 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    11701 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    11761 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    11821 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    11881 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    11941 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    12001 ctgcctcct
//
LOCUS       DACCQP010000082        11995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCQP010000082 DACCQP010000000
VERSION     DACCQP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.085"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            349..957
                     /locus_tag="IVO87_002331"
     CDS             349..957
                     /locus_tag="IVO87_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7450490.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1125..2762
                     /locus_tag="IVO87_002332"
     CDS             1125..2762
                     /locus_tag="IVO87_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7450491.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2834..3946)
                     /locus_tag="IVO87_002333"
     CDS             complement(2834..3946)
                     /locus_tag="IVO87_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7450492.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(4021..5394)
                     /locus_tag="IVO87_002334"
     CDS             complement(4021..5394)
                     /locus_tag="IVO87_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7450493.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5615..6232
                     /locus_tag="IVO87_002335"
     CDS             5615..6232
                     /locus_tag="IVO87_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450494.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6330..6785
                     /gene="argR"
                     /locus_tag="IVO87_002336"
     CDS             6330..6785
                     /gene="argR"
                     /locus_tag="IVO87_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7450495.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6803..8305
                     /locus_tag="IVO87_002337"
     CDS             6803..8305
                     /locus_tag="IVO87_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7450496.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8400..8606
                     /locus_tag="IVO87_002338"
     CDS             8400..8606
                     /locus_tag="IVO87_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450497.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            8692..9093
                     /locus_tag="IVO87_002339"
     CDS             8692..9093
                     /locus_tag="IVO87_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7450498.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            9248..10579
                     /locus_tag="IVO87_002340"
     CDS             9248..10579
                     /locus_tag="IVO87_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7450499.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            10601..11227
                     /locus_tag="IVO87_002341"
     CDS             10601..11227
                     /locus_tag="IVO87_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7450500.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            11410..11991
                     /locus_tag="IVO87_002342"
     CDS             11410..11991
                     /locus_tag="IVO87_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7450501.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
BASE COUNT         4140 a         2147 c         2503 g         3205 t
ORIGIN      
        1 aattgttgta acaattactg gtttttttgt attttttaca tttttttaag agaatttgat
       61 aaaaatcaaa aatttttttg ctttttttta tggattattg cagtgaatgt tacagaatct
      121 gcgtttttca ttcaaaaata aaaaaaacat agaaaaacga ttactttgct tatacaacaa
      181 ggcttttatg gttttgtatc aaaaaaatat ttgtttaaat gttacaaata ttaaataatt
      241 cgttattttg tcattaaatg tcatgtttct tttacctatg aaatatttct aaatgataaa
      301 gttaatagcg tcgaaagaat aaaaaacgga gaaaaaaagg agtttaatat gaattcattt
      361 aagaaaattg ttttaggtac aacttttgct gctggtgcta ctgcaatgtt tgtaggaact
      421 acaaatgctc acgctgatga agtttataca gtaaaatcag gtgattcatt atctaagatc
      481 tcacaaaaat ttgcaggaga caactcaatg atcgatgcaa ttgctgaaaa aaattcgatc
      541 gcaaacatca accgcattta tgttggtgaa caattaacaa tcccaacttc aaatgattct
      601 tcagcaacaa cagaaaacaa aacaacagaa aatacagcat ctacaactga aacagcaacg
      661 caagaacata catacgttgc acctgttgaa acagttgaag tagctcctgc tgccccagct
      721 gctgcaacag cgccaacttc aagctctgca aaagaatgga tcgcacaaaa agaatcaggc
      781 ggttcataca cagctactaa cggacgttac atcggccgtt atcaattaga tgcttcatac
      841 ttaaacggcg actattcagc tgccaatcaa gaacgtgtag cagaacaata tgtaacatct
      901 cgttatggtt catgggatgc tgctaaaaca ttctggttag caaacggctg gtactaagcc
      961 gataaaagga aaatgatgaa accgagaaac ttgtttattc aagttctcgg tttttttgtg
     1021 tgaaaaataa ctcgtttgct tcccacagcg cagcagtaca aataaaaact cgaataatgt
     1081 ataataaaga aaacgttttt ttgtttaggg aatgaggagg agccatgagt acaaaagaga
     1141 gtctcgaatt aaacgaaatc acaagtggaa atccactgtt atccagtatt cgatcgattg
     1201 tagaaacgac attttacggt aataatgttc atgaagtttt tgataggaag acagcttatc
     1261 aactagcaaa agggagtcca ggaacgatca tcacagattt aacaatctca catgctgaag
     1321 aactagattt accagctgat gttaggacac tagtattcaa tgatggaagc attgtgggaa
     1381 gaacagcaag tgctcggcgg atctttgagg acttagacaa ggaacaaagc aaatacgaga
     1441 aaattttaag agaagctgtg tatcaaagtc gcaagcgcca gttttaccat acaaaagtaa
     1501 tcgttggcct atctgaagaa ttttctgtgc aaagtcattt gctgattcca gaaggatttg
     1561 agaacaattt gtattcttat ttgctgaatt ttcagccagc gaattcgaag atcttagaga
     1621 cttatcaaaa gtctgtttcc tttgatgagg gagatcttta tatcttttgt gatcccgaat
     1681 ggacacatga aacatttcca aatggactca ttttgtttga tgctctccat aatacagcgg
     1741 ttgtgctagg attacgttac tttggagaat taaaaaaagc gactttgacg cttgcttggg
     1801 cattgggaaa tcgcaatggc tttattgcct gccacggtgg gatgaaacaa tatgctagta
     1861 atgaccagac ctatacgatg gctgtatttg ggttgtccgg ttctggaaaa tcaacgatta
     1921 ccttagcaaa acatacggaa ggtcactctg tttcgatctt gcatgacgat gctttcgtta
     1981 tttctcgaaa aaatggtttt gctactgcct tggaaccttc ttactttgat aaaacacaag
     2041 actatccgtc tgatgatccg gccatttctt ctttccttac ttgtcagaat gtgggcgtga
     2101 catgtacaag taaaggagaa aaagtcctag taacaggaga tatacgaaac ggaaatgggc
     2161 ggacagtcaa atcacgttat gcgacacccg accgagtcga tcatcttagc gaaaagatcg
     2221 atgctgtttt ttggatcatg aaagatgatt cgcttccgcc aattatcaag atcaatgacc
     2281 cagttttagc agcgtctttt ggtgcaacat tagcaacaaa acgatcagac gcggaaaata
     2341 tcactacaaa aagacagcta gatgagctag tcattgaacc atttgctaat cctttccgtg
     2401 tctatcctct tagggaagac tatcaagctt ttcgagagtt gtttttgaaa cagcaaacag
     2461 cctgctatgt cctgaatact ggatatttcg atgataaaaa aatcaaacct gaaacgacat
     2521 tgtcaatcgt tgaaagaatc gtgcaacaaa cggcagaatt caagtcgttt ggtccatttg
     2581 aagacatcca ctatttacct ataccagatt ttcctgtttc ttttgaggat acgtcctata
     2641 aaaatctggt gaaacaacga cttgatattc gtttacgatt cattgaagct cagataggaa
     2701 aagatctctt gccagatgaa gcaaaagaaa gtttggagaa tttgattcag aaattgaact
     2761 aaaataacag ctttttcaag aagtaatcca tgcgcaatat cgctcgggat ccttacttga
     2821 aaaagctgtt tttctatgat ggatttttcg taatataggt ggagatgtcc ttttgataat
     2881 cttccgtagg ccaatgacca gtatttaaga agttggctaa tagatagccg atataaggac
     2941 ctgtggtcaa gccagaagag cctagaccgc tagcagccag taagcttgga tcatctggca
     3001 gtacgccgaa aaatggggaa aaatctgacg tataagcacg ggtacctaca cggaactcgt
     3061 atggccagtt ttttatctga tctggatcag tcaagaactg ttctgtactc ttagtcaatc
     3121 ccttataagc ttcgctagta ggagccaaat cccagccttg atcgttttca tgggtagcac
     3181 ctaaaaggat ctttccatcc atgaaaggaa tgaaatcagc ttctccgtca agcatagcaa
     3241 caggccagtc ttgactcatc ttataagatg tctgaaatgc taagagctgc cctttttgcg
     3301 gacgtatatc tgcagcatag cctaattctt gtaataaagg cttaagtgcc gggccaggtg
     3361 tcaaaattac acgatcagct gtgtattctc caaacggtcc cttgatgagc caagaatcgc
     3421 tcttcttatg caaagtgact tttgcctgga tctgctccat ttgtttttgt gctaaacgct
     3481 gaccaagatg ttctaaatat gcttttccat ccagacgacc accaccactg atacgcagtg
     3541 aaactgctgg tttcaataac ggaagtgctt gagatgtctg ttcaggtgaa agccattcga
     3601 tgtttccaat ttctggcgct tcttcttttc gtttctcggc aagaacagcc aattcagcta
     3661 aatctttttc aggtcgaagc aaaagtgtcc ctgattgtgc gtaaatcgtt tgatcaagtt
     3721 ggaaatcttt tataagttta gggaaaaaag cagcgccgtc tttagccaga cgataccatt
     3781 gtttgtttct gcgtttcgac aaccaagggg agataatccc agcgctagct ttagttgcct
     3841 gtcctgtccc ttcatcaaat aaagcaacat tgaattttga tgtatctaag taactggcta
     3901 gggtcatacc aatgatcccg ccaccgataa tggcaatttt ttccataaaa aagcctcctt
     3961 ttacaaacaa ccattttcac acaaaaaaac ggcagatgca acacctgccg tatatggaat
     4021 ctacgctttt ttagcaaaga acagataata ggcgaatcga gcaaagctta cgagaagcaa
     4081 cagcaacaag acgcctaatg ctgcttttga tcctaatgta gtagacactt catagttatg
     4141 ggaagtatga tgctggacgt aattgataat gaccgctact aatgaagtag ccacagcgcc
     4201 agaaaactgc tgcaaggtat tgaacattgc gttcccgtct ccttgtagtt cttcattcag
     4261 tgcattcatt ccggtggtca tcatattgct gtaagataat ccgataccga tcatataaaa
     4321 cacatgacaa gcaatcaata cacccactga agcgtgtcct atgagtagga ataatgctag
     4381 ccaaccaatc aaagaaagaa tcagcccagc aaaaattggc ttcttcggac catattgatc
     4441 cagcattttc ccggaaaaag gagaaagcaa agcaccaacc gctgcgccgg gaagcatcgc
     4501 taatccagcg acaaaaggac tacttccttg aacgatctga aggaaattcg gcaagacaaa
     4561 agatacacca agtaataaaa actgataaac taagaagcca atcaaaaaca agcggaatgt
     4621 tttatttgaa agaatagaca gtcggatcag tggatgctcc acttttttac ttcgatagta
     4681 aaaaaggaac cagccaatac cgccgatgat taaagcaatc cagctggaaa ctgttcccaa
     4741 cgtactcaaa aatgttagac ttccactaaa caaaagacaa attcctaaaa cgcttatcca
     4801 atccaaataa ccactttttt gtactgccat ctcgggaata gcaaaccagc cgataatcaa
     4861 agaaagaatc aaaacgggca caagaaacag gaagatatga ttccatgtaa aatgtgaagt
     4921 cataatgcct ccataagtgg ggccaattgc tggagcaatg gccgttgtca aagtaccgac
     4981 acccatcatc gcgcctcgtc gattgagggg ggcaaatgtc aagataatat ggaacatcaa
     5041 cggaagagcg atacctgtcg ctgcaccttg gagcaatcga ccaaacaata ggaccatgaa
     5101 ggtaggagag aagaaatcga tcattatacc tgccaagaaa agcagatttg aagctaaaaa
     5161 gagttttttg agactaaaat tgcgattcaa ataagtagac agcggaacaa tgatcgaaat
     5221 catcaaaaga taaatcgttg ttacccattg gacttgtgca gtcgttaaat caaactgtct
     5281 gataagagtt gggaaagtta cattcatggc cgtctcgatc aatacgcccg caaaagacat
     5341 catccctgtt gcaattactg caggtactaa aagcagctgt ttttgttttg tcatttacat
     5401 cacctctaaa aaatttcgta aaaaaagaga gtgatttcta ttataaaaat agaaaccgcc
     5461 tctctctcga cacgccgaaa cgtgttatct gtacgattta tttacttcac ttattctagc
     5521 acaaactgaa aaatagtgtc aacacgatta gccaagtcac tcaaaaagag gtatgataat
     5581 aaataactaa atggaatttc gcggaggaag aaaaatggaa aaaattgaat ggcgtaaaga
     5641 caagagctat agtatgaaaa aaagcccgca aatactagag gttcctaaaa aatcattctt
     5701 tagcatcaga gggatcggcg acccgaatca ggaagacttt aaacgaagag tagcctgtct
     5761 atatgcagtc agctatgcaa tacgcatgtc gcctaaaaaa aattggctga ttcctgatta
     5821 tcagatgtat actgtctatc cactggaagg acaatgggga ctgcaagaaa agtatttgca
     5881 tgaaccagtg atgaaaaaag aacatttttc ttatcaattg atgatccgac agcctgactt
     5941 tgttacagaa gcgatagcac aagaagccat tcagcgttct agcaaacttc cggaagactt
     6001 gaaagaacaa gtagtattca aaaagatgga agaaggtctt tgtgcccaaa tcttacacgt
     6061 cgggccctat gccgaagaac cagcaacgtt tgagaaatta gaagcattca tcgcaaaaga
     6121 aggctatgaa agagcttcta aagaacacaa agagatttac atcagcgatc caagaaaatc
     6181 agcacctgaa aaaatgaaga cgattttgcg tgttaaaata gaaaaaggtt agcaacctga
     6241 atgaaaacaa aaaataaaag taaaaaatac caaaatatga agaaatatta cttatctttt
     6301 gctataatca acattgagag gtgtctaata tgaaaaagaa acagcgacaa gcactgatcc
     6361 gccagatcat tacagaacaa cctattggga cacaggaaga gctgcttgcg cggctgcatg
     6421 aagcaggcgc cgatgtgact caagcaacga tttctcgaga cattcgagaa atgaagttag
     6481 taaaaagtca aaatgaaaac aaaatagtac gctatacatt atttaaccag ccatctgttt
     6541 ctttaaatga agaacgtttg cgtacagcaa ttagacgcga ggttttgcgt atccagtctg
     6601 ttcagttcat ggttatcgta ttgactgaac gaaatggtgc cgatgtggta acgaattggc
     6661 tagatgaagt ggcttaccct gaagttgtcg gaacgatggc cggtgtagat acattcatta
     6721 ttatttgccg ctcagaagaa gaagcacagc gatttgcaga aaagctggaa aaaatgaggg
     6781 aataaatgag gaggactttt atatgaaaga tatgaaagcg ctgcaaaatg gatcggatat
     6841 tcgaggaatc gcattaccag ttgaaggata tgaagtaaac ttgacgacag acatggtaaa
     6901 aaagatcggc tggggactag caaactggat aaagaaaaag aagaaatcaa ccgatccaaa
     6961 taaaaaatgg acgattggga ttggtcatga cagtaggata agcggaccag cactgaaaga
     7021 ggcgttgatc caaagtctga aagaacaagg gatacatgtc atcgatttcg aattagctac
     7081 tacgcctgct atgtttatgt ccactcaatt tgaacagttc tcgtgcgatg ctgcggttat
     7141 gctgactgct agccatcttc cctattatta taatggaata aaattcttta cctcagaagg
     7201 aggagcagaa aaagaagata ttgcttttat cctatcacag cagcaaaaaa taaaaagcga
     7261 acaaactgga agcgtagaaa aagcagacct tttgacacct tatgcgcaag atttagtcac
     7321 aaaaattaaa aaggggattg gaacactaga aagcaaacca ctaaatggtt ggaaaatcat
     7381 tgttgatgcc ggaaatggtg ctggtggatt ctttgctgaa aaagtattga atagcttagg
     7441 tgcggattca acaggttccc aatttttgga accagatggc cgatttccta atcatatccc
     7501 aaatcctgat aataaagaag cgatggaaag tatcaagcag gctgttttag caaatgaagc
     7561 agatcttggg atcattttcg atacagatgt ggatcgttct gcagttgtca gtgcttctgg
     7621 aacagtgatc aatcgaaata atttgatcgc agtattaagt acaatcgtat taaaagagca
     7681 tccaggaaca agaatcgtga cgaattcccc tacttcttct catctacaaa gattcattga
     7741 agaaaaagga ggcatccaag ttcgttatat ttccggctat cgaaatgtaa tcaataaagc
     7801 attagaactt aaccgtcttg ggaaagatac acagctagcc atcgaaacaa gcggacacgc
     7861 tgcattcaag gaaaactatt ttctggatga tggtgcatat gtcatcgcaa agatcctgat
     7921 gcttttgcca gaattaaaaa gaaaaggtat cacattagat gaacaaatca aagagttgaa
     7981 acagcccgta gaagtacaag aaatccgatt aaaattgttc agcgatgaac cacgcgaaca
     8041 aggaaacaag atattagaag aaatgaagcg actgttggaa gaagaaaaag ggatgaactt
     8101 cgacccggaa aatgaagaag gtattcgata tgatctagaa gagccatacg gaaacggctg
     8161 gtttttgatc cgtttaagct tacatgagcc gttactagta ttacagatag aaaatgatga
     8221 attcggaaag aacaagttgg cgttagtaaa attagcaaaa ttattcggac atttttctga
     8281 tcttgaatca ttaacagcag aataaaccag tctttttgaa aggctagttg cttggacggc
     8341 aaaaaaagtg gtatggttaa atcgagtgct ggtagggaga ccagcaaaag gaggaaaaaa
     8401 tggtaacgaa tatggttatt tttttaacag tattggttta tggggtcaac tttctggtat
     8461 atatgtatct taaaatcaat catataaaag attatgtaga aaagattgct atttattttg
     8521 gaacaaatat gacgctgttg ttcacgagta gtattttttt gttttttgga aaaatagtag
     8581 aagacggcat tctggtattc gaataagcag aatttttgta gaaagcgcct tacttgtcat
     8641 acactataat tatagccaag tagcaaatgg tcaatcgaaa ggagcagtac aatgaaaaaa
     8701 gcatacgaaa cagcaatgat caaccatggc gggcgcaatg gagaagtcga ggcaccaaat
     8761 ggctcgatgc atatgaaaat cactcctcca ggaatccatg cagaaggaac aaatccagaa
     8821 caattatttg cagcaggtta tgcttcttgt ttcaatgggg cggtacaaca tatgatcaaa
     8881 gaagcaggaa tgacagcaga gtccacagta aaagcacgtg tctctcttta taatttagat
     8941 gatggcggct atcagatcgg tgtagtatta gaagtgcata tcgatggcat cagcgaagaa
     9001 gaagcccaaa aaattgcaga agatgcgcat gaattctgcc cttactccaa agcaactaga
     9061 ggaaatatcg atgtagaagt aacgattgtt tagaagaata aaggaaatcg aaccagacta
     9121 aaaacgtgaa aggaccgctt tttgtctggt tttttttgac aaataaagca gatgatttgg
     9181 agaatagaca attatcggtt atcatatttt tgtaccaatc ggtaggtaaa aaagaagcga
     9241 gggaaaaatg atggaaaaat atcaaaatat tgtgatcggt tttggaaagg gcggtaagac
     9301 tttagcgaag gcgttagctt caaaaggtga atcggtttta gtcgttgaaa agtcaacacg
     9361 tatgtatggc ggaacatgta tcaacatcgg ctgtattcct tctaaatcat tgattttcaa
     9421 tggagaaaga ggaatcgact ttacagaggc tgttgctcgg aaagaaaaat tgacgggcat
     9481 gcttcgcgcc aaaaattatc atatgatcag cgatgaggca actgggacag tgatggacgg
     9541 tacagcacgt tttctttcta accatcaaat cgaagtgacg aataatggtg aaaaagtgat
     9601 tgttgaaggc gaacggattt tcatcaatac tggttctgaa ccaatcattt taccaatcaa
     9661 aggattgaat acaagtcgct acttgattga ttctacgcaa gcgatggatc aggaacaatt
     9721 accggaaaaa ttagtgatca tcggggctgg atatattggc ttggaatttg catcaatgtt
     9781 caatgaatac ggatctaaag ttgtcgttct tgatgcccat tctgaatttc ttccacgtga
     9841 agatgaagat atcgcgcaaa tgattttaga agacatgaca aatgcgggaa tcgaattcca
     9901 tctaggtgta tctgtagatc aagtagcaga ccaagaatca acagctgccg tcacctttac
     9961 agaagatggc caagaagtaa caatccaagc gtcaaaagtt ttggctgcaa ctggaagaaa
    10021 accaagtaca gcatcacttg gattagaaaa tacagatgtt aaactaacag atcgcggagc
    10081 aatcgctgtg gatgaatacc ttcgtacaga tgcagacaac atttgggcga ttggagacgt
    10141 caaaggcgga ttgcagttca cttatatctc tctagatgat tatcgaatca ttatggatca
    10201 gttgaagggc gagaataaac gaacaacgaa taaccggaaa gctgtgcctt acagtgtatt
    10261 tatcactccg actttatcca atgtaggttt aacagaaaaa caagccaaag cacaaggcgt
    10321 caattacaaa ttattcaagt atatgacaag cggtgtccct aaagcacaag tattagaaga
    10381 tcctaaaggt gtattcaaag ttttagtcga ccctgaaact gacttgattc taggggcaag
    10441 tatctatgcg gaagattctc atgaagtcat caacttgatt tcgctagcta tgaatggaaa
    10501 acttccttat acgttattga gagatcaaat ctattctcat ccgacaatga gtgaagctct
    10561 aaatgatgta ttaaaataaa taacgaatgg agaatccacc atgttcaata ataaaaatac
    10621 agcagtagtt ctcactgacc cgcaagtcga gttcttgaaa ccagcaggcg gtggctttgg
    10681 tttgacaaaa gatatcttag aaaaatacaa tacgatcgaa aatatgattg aaatggtaaa
    10741 acaagcaaaa gaaaaaggat acaaactctt tatttctcct cattattttt acccacatga
    10801 ccataaatgg caatttggcg gtgcaggaga acatatgatg cttgagcagc agatgtttgc
    10861 tagaaaaagt caatatgaat cagtggaaga aggttcagga gccgatttta tcgaagaatt
    10921 gaaaccttat ttggatgaaa atacgatcat cagctctcct cataaaatct ttggaccaga
    10981 aagcaatgat ttggcgctcc aattgagaaa aaatggaatc gacacagtaa ttcttggtgg
    11041 aatgaacgga aacttgtgta gcgattccca tatgcgtgaa ttggtagaga gtggattcaa
    11101 tgttgtggta gcgacagatg caattggtgc tcctggccaa gaagcaatgg atgcagcgtt
    11161 gacgaacttt ggctttattg cagatcaagt gatgacaaca gaggaagtat tagagcaact
    11221 atcataagat aggaattgct tttgtcctcg cagttcttga atcaatgaac atctagctat
    11281 atgagaacag ttttatagtg agggctgtgt cagataattt ggcacagtct tttttaaacg
    11341 attaagaaga aaaaatgatg aatttactgt ataatatagg actatgagaa atgaaaaagg
    11401 agaatatcat tgaaaacaaa ggaaaagatc ttggaagcga ccaaagatct tatttataaa
    11461 aaaggctatc atgaaacgtc aatccgagat attctagcag cttctgatac aggaaaaggt
    11521 caattttatt attacttttc ttcaaaaaaa gaagtttgtc tagcggtgat ccatgcgcat
    11581 atggacgtat ggcagacaga attgttcgaa gacatcttaa atgctggaaa acagccagaa
    11641 gaagcattag aagaaatgtt ggactggatt ttcgctgatc atgagacaca gatcacttat
    11701 catggctgtc ctgtaggtaa tttgattatt gagttgagta cagtggatga agaataccgt
    11761 ctgttgctac tagaattagt caatcaatgg gtcaagtata cgaaacaaaa tatcctggca
    11821 ctttctgaag aaaaactgtc cgatgaagaa gcagaccgtc aagccaaagc gttgattgct
    11881 gaaatccagg gaagtatctt attgttgaaa gtcacacagg atctatcact acttaggcag
    11941 aattttcaat tgatcaaaga aagattgtta acttcgcaaa aggttcttta ataat
//
LOCUS       DACCQP010000083        11757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCQP010000083 DACCQP010000000
VERSION     DACCQP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.086"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(116..640)
                     /locus_tag="IVO87_002343"
     CDS             complement(116..640)
                     /locus_tag="IVO87_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450502.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(633..1106)
                     /gene="tsaE"
                     /locus_tag="IVO87_002344"
     CDS             complement(633..1106)
                     /gene="tsaE"
                     /locus_tag="IVO87_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7450503.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1229..2209)
                     /gene="pta"
                     /locus_tag="IVO87_002345"
     CDS             complement(1229..2209)
                     /gene="pta"
                     /locus_tag="IVO87_002345"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7450504.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2362..3042)
                     /locus_tag="IVO87_002346"
     CDS             complement(2362..3042)
                     /locus_tag="IVO87_002346"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7450505.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3168..4037
                     /locus_tag="IVO87_002347"
     CDS             3168..4037
                     /locus_tag="IVO87_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7450506.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4063..4578
                     /locus_tag="IVO87_002348"
     CDS             4063..4578
                     /locus_tag="IVO87_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450507.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4625..5188)
                     /locus_tag="IVO87_002349"
     CDS             complement(4625..5188)
                     /locus_tag="IVO87_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450508.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5243..5422)
                     /locus_tag="IVO87_002350"
     CDS             complement(5243..5422)
                     /locus_tag="IVO87_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450509.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5499..6008)
                     /locus_tag="IVO87_002351"
     CDS             complement(5499..6008)
                     /locus_tag="IVO87_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450510.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6104..6484)
                     /locus_tag="IVO87_002352"
     CDS             complement(6104..6484)
                     /locus_tag="IVO87_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7450511.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6814..7563
                     /locus_tag="IVO87_002353"
     CDS             6814..7563
                     /locus_tag="IVO87_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7450512.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7747..9024)
                     /locus_tag="IVO87_002354"
     CDS             complement(7747..9024)
                     /locus_tag="IVO87_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450513.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9103..9747)
                     /gene="pcp"
                     /locus_tag="IVO87_002355"
     CDS             complement(9103..9747)
                     /gene="pcp"
                     /locus_tag="IVO87_002355"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7450514.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9946..10890)
                     /locus_tag="IVO87_002356"
     CDS             complement(9946..10890)
                     /locus_tag="IVO87_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7450515.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10892..11578)
                     /locus_tag="IVO87_002357"
     CDS             complement(10892..11578)
                     /locus_tag="IVO87_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7450516.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3359 a         2482 c         2057 g         3859 t
ORIGIN      
        1 tgaacaccgt ttattcaaaa aaaatggact gcgacacgac ttttgtcaca gtcccttcgt
       61 gtgtttttct gccttctcaa aaggatgaca gtttctcact cttttcctat ttcttctatt
      121 caatcaaata actcatttta tatacatcta aaaattcacc tagatctgtt cttgcccctc
      181 taggcatgac cgcttcgatt tggaagccca tttttcggta aaggtgtacg gctctttcat
      241 tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc catttccttc gcccaactga
      301 tgatttcttc tagcataaga gtgcctagtc ccattcccca atattcctgc agaatactga
      361 taccgacttc tcccacgtga gacaaacgaa attgactgtc tgccttgaca gatgctgttc
      421 caataatcgt cccttcatag attgcaagca gcaataaatt attattgctt tcgtatatat
      481 aatccaactc ttcttctaag gtttctggtg gtaacaggag ttctgcttca tccaaaacta
      541 aaaattctgt ctccttatta acttgtccca tcatcaaaag aatgtctttt gcatctcctg
      601 gcactgcttc ccgcaagatg atcccaactt cttcactcat gattttcagc ccatttttct
      661 agaattcgct gtttgaaggt ttcactttgc tccccgtatg agattagttt cacataccgc
      721 ttttctaaat ctgtatcact tttttctaaa atcagttcca aataatcctc cggcagtgct
      781 tcttctaata gatttcccca ttcaacaacg gaaagcccat caccttcaaa atattcatct
      841 aaccctaaat ctgctccgct agctgctacc cgataaatat ccatatgata caacggaatc
      901 cgaccttgac tatattctct aataatcgtg tatgtcggac ttttgatcat ttgcgttatc
      961 cctaatcctt gggcaattcc tttcgtcaac gttgttttac ccgcacctag atcaccagtc
     1021 aatatcagat tgtcacctgg cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc
     1081 attgttaatc ctgatagttc gatcattttt catcttctcc cgctcttctt tctttttgcc
     1141 agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg
     1201 ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa
     1261 tcgataattt atatacatct tcttcatttg ctccacgaga caaatcagaa accggtttgt
     1321 tcaagccttg tagaattggt ccgattgctt caaagttacc gaaacgctga gcaattttat
     1381 agccgatatt tcctgactgc aattctggaa atacaaagac tgttgcttga ccagctactt
     1441 ctgaatctgg cgctttcagt tgtgcaactg aaggaacata agaagcatca aattgcaatt
     1501 cgccgtcgat catgtattgt ggcgctagtt ttttagcgat tttagttgct tcaactactt
     1561 tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa gctcatcatt gctacttttg
     1621 gttcgatatc gaacaattct gctgtttttg cagaatctac ggcgatttca gccaattctt
     1681 gcgcagttgg attcacgttg atcgcacaat ccgaaaagac atatttttct tgatctcttc
     1741 cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct
     1801 gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc tccagagacc attccgtctg
     1861 caatccccat atacgtaagc atcgtaccaa agtaattcac atcttttaaa atcttttctg
     1921 cttgttcttt ggatgccttt ccatttctac gttcaacaaa agtatccacc atttcatccc
     1981 acttatcgta attatcaggg ctgatgatcg taaagctaga tgttttgatt cctctggcat
     2041 gcgctgcatt tcttatttct tcttcgttcc ctactaaaat gggttccaca agttcttctg
     2101 ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt agcttccggg aaaacgattt
     2161 tgatcccacg gcgaacaatt ttaaatctta aactatcaaa caattccaca agatgacctc
     2221 cagacgtttt tatatgtatt atcatacact attccagaaa aaaagaacag tttattttat
     2281 ctgtatcagt caaaaatgct ggaaaaatta agtttttcac atacaaagcg aactgcacta
     2341 taacaggaaa gcgctgtttt attacactgt ttcaggcagt tgccaatcaa tcggttcttc
     2401 tcctaacgca aggagcgcat cattcgtttt cgagaagggt ttggaaccaa aaaagccacg
     2461 acttgcagat aacggactgg gatgtggaga tgttatgatg atatggcgac tcttatcgat
     2521 cattttgatt ttttcttgtg ccggttttcc ccacaagata aacacgattg gtttttcccg
     2581 ttcatttaat ttttcaatga tcgtgtctgt taatctttcc caacctttcc cgcgatgaga
     2641 atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt aaaaggactc cctgtttagc
     2701 ccatgacact agatttccat gttgaacagg tgcgattcca agatcatcat aaagctcttt
     2761 atagatattt cttaacgatg gcgggatctt tacgccaggt tgcacagaaa aagataagcc
     2821 atgagcttga ttttctccat gataaggatc ttgtcctaaa atcaccactt taacttcttc
     2881 ataaggagtc aattccaatg cttcatagat atgatacata tctggatgga tcttttggga
     2941 cgcatattct ttttttaaaa attctcttag atgcagataa taatcttttg aaaattctga
     3001 ggacaaaact tcttgccaac tgttatgaat gatttctttc aaaaactcca cctcctaata
     3061 aaaagaataa ctcacatttt cttcaatcac aagggacaat cttcaccctc agcacttgat
     3121 taatatggta aactaggaac aagaagcaga aaggaacgat aatcattatg atcaaattaa
     3181 ttgcatcaga tatggacggc acattattag atgcccatat gagtgtttct cctgaaaatg
     3241 ccgaggcgat acgttttgca aatgatgccg gtgttgagtt catggtcgct actggtcgaa
     3301 actatcaaga agctcgcgca gctttagatg aggtaggaat cgattgtgcg atgatcactt
     3361 tgaacggggc acaggtattt gataaagaag gaaattcatt gtttaccgtg tcgattccaa
     3421 ataatcaagc aatctctgtt ttagatattc tagatgctaa cggtatttac tatgaagtag
     3481 caaccaacga tggtctttat tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca
     3541 tggtggctac ccatttacct caccttacgt ataaaatggc gattgccatg gcctcagcta
     3601 agctggagtt attgcacatc acatacgtgg acagtattcg tgctattttg gaagaaaaga
     3661 atctggaagt tttaaaaatt atttgttttc atacagaagg gccagctgta ttaggtccag
     3721 ttggaaagca gatttctctt ttaggcgatc tagccgttac ttcctctggt caaaacaata
     3781 tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc
     3841 gtgggatttc tttagaagaa gtcatgacga ttggcgataa cttcaatgat gtcagcatgc
     3901 tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg
     3961 ccaaatatac aacggacaca aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga
     4021 ttgcagaaga cttgtagttt tatctagaaa agagggatat agatgtcaga atttttcatt
     4081 caggaaaagc agcttggacg tgcaactcgg acaattgtca aaaacgaaaa aggacattcc
     4141 ctgtttttga tggttggtcg ctggggaaca aagggcgatg cattgtctct ttatgccatg
     4201 aatggcgatc tcgttgctca catcaagcag ataagcctga cattcggtac gcgttttgag
     4261 ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa tattcaattg gcccggtgac
     4321 ttttattata ttcgtagact tcactggaca gtccaaggtg atatctataa tcatcaatat
     4381 caaatccatc atttcaatca gcaaatcatg aaaatggata aagcaacact tttttcagga
     4441 gattactatg tattggatgt ctcaaatgat gaagatgctc cagtttgtat ttgtattgct
     4501 gcggttttag attactggct ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac
     4561 cgacttaact taagttaaaa agatgtacct gaaatgcgga aatacgcatt tcaggtacat
     4621 ctttttagat attcttttgc atatgatcat aaagatgacc actgatcatc cgctgaccga
     4681 caactttgaa gtcatggcgt tcgtataatt ttttagcctt tggatttttt tcatccacac
     4741 ttagaccgat gacggagcga ttagctttct tcgccaattt aggtaatgct tctaataatc
     4801 gcgaaccgat gccttgtcct ctaaaatctt ctgatacact gatagagtcc aaataccatt
     4861 cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga cgcatctagc tgatatttct
     4921 ctaagatagg taccagtggc tgatcgatga ttgattcttc ttcatctgta taaccgtaag
     4981 caactcctgc aactttaccg tctatttctt ctacgatacc tcgtgaataa ctgtaacgat
     5041 aatttggatc agtgattgct tcttctaaaa tctctaaagt cttttgttcc cctgtctgct
     5101 tcaaaaatgg cagttccata tcttttagaa tcactaagat caatggggcg atttctggag
     5161 catcttcttt tctcgcaaaa cgaatcatag tttcctctcc ttttctccat tctaacatgc
     5221 agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc aatcttttca gtaatgaaat
     5281 aaccatgagt aacgattctt gccgcttcag ttcggcaata agggcagacg actggcattt
     5341 ctttatcatt atgttcgact acattattga cttcttcgta tgcaaatttt tttccgcaat
     5401 tagggcattt cattttttcc atgttatctc aacttccttt catctttact ctcttagctt
     5461 actcttatat tgtaaaaaag agaagcaata tccttcttct atgcagatat ccatacctga
     5521 cggtcctgtt ctccatctat taatcggact ttataataag gacgttcatc tttgactaga
     5581 agtgtccact ctttcacttt tgcttttttc ccgtagtagc tgactgcttc cttcatcgct
     5641 gcttgcggtt gtttgaactc ttccgttttt tctatatcaa aaagatgttc tgcggccttt
     5701 tccgtgtcat ttcgtaccgt tgttttccca gtggatggat tgactgctat gacttgtcct
     5761 tcacaaaaaa actggtattc atatgttgca ttcgtaggat tctccaaatc tttttcaact
     5821 gcaaaatgaa tagcagatat gttatgttct tttgtttcgt tttttacgat tgctgctgct
     5881 tttttcaatg agatccgata ttcctggtca ctaatatcat ccatgtgaaa agcggcattg
     5941 agcagtcccg tcataccggt aatccctatt acgatgaggg cagcaacacc aaacatcatt
     6001 tttttcattc ttcattctcc tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg
     6061 aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta tctttattcg tgcagttcta
     6121 atggcaatcc gtctggatca aagaaaaacg tcattttttt tcctgtaaag gtatcttctc
     6181 taatcggttc tgattcgatc cctaaggact gcaattctgc aattgtttct tcaatatcgt
     6241 ctactttgaa agcaagatga cgcaagccac aagcttctgg atagtttggt cgttgtggcg
     6301 cattagcaat tccaaaaatc tccaattcag cacttcctag tttcagatct aatttatagt
     6361 catctcgttc tttgcggtaa ttttctctga ttacttcaaa tcctaattta ttaacataaa
     6421 aatcacgtga tttctgataa tctgaaacaa tgatggctat atgatggatc gttgtaaggt
     6481 tcatatgagc actcctattc ttcgtttaag ctcggatcta tattaatctc cagttccatt
     6541 gtaaatgtgt cagattgaaa aaaatagctg tttttccttt ttataagaaa aaaggcaaaa
     6601 aaaacagaaa gtaggtcctc caagacactc ttcctgttcc caatataata aatgaaagta
     6661 agaaattata ttttcaagaa aacaaacaga aaattatcat aacacaaatt atttataatt
     6721 gaaagactag ttttttttca agaaaagcat acaatagatt gtgttagtaa atacagaaca
     6781 cactacattt agtgtcttaa ggagtcctca tacatgaagt catattttca gtttttagct
     6841 catcagctaa aaggctggcc gcaacaaaat tattatcttt tctttttcag tcttggctgc
     6901 caggtcatga ctcttgtcaa tagtcccatc acttggctgt ccgttattac atttatcggt
     6961 accacgcttg gtgtattgtg tattttatcc atcaatgcgg caaaatcagt caacggtatt
     7021 ttaggtattt tgtctgcgat ttgttttatt atcgttgggt tttcagcaaa gaactattta
     7081 agtatcggtg aacagattgc ttatatgatc acattggata tccccgtatt gttaagtaaa
     7141 gaatggaatc ataatatggc ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc
     7201 gcaatcatca gtactttcct tgtctatctt gtttcaggat tcctgattgg tcgttataca
     7261 gatgatcctc ggccgtgggt agatgccatt gctttttcta ttagcttgac tgctggtatc
     7321 atcagtttct tgcgttacaa caaccaatat tactggtggt tagcttcagg gcttgctcaa
     7381 atggtccttt ggtatatttc atttacgcat ggatctgcaa gtttggcgat gttcgtgaac
     7441 agctctgttt acttgctgaa tgatgtcctt gcatttaccg tttcaccttg gtataaccaa
     7501 aaagaacgtg aacgtatgca aaaaatggaa gaagaatact acaacgcaca acaacacgca
     7561 taaaaaaact gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
     7621 ataaacggcg gaatcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     7681 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
     7741 tattttttat agtaattcct tcagatactt tcttgcctca tcattgaaac gcagtgattc
     7801 ttctaacttc tgataatatt ccaacgcttg tttctgagct tcagtaaaac gataatgttt
     7861 tcctttccat ttgtcgattg tctcttgcac atttccagat tggatatctt tcactactag
     7921 ttgccatttt tcttttgaga ctgtacggtc tttcttatca gcagattgta tagcttctcg
     7981 ttctttacgc aatactttta aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa
     8041 cccttctgta aaaccatagc gacaatattg cttccaaata aatagataga tttttggttg
     8101 gatgaatcgt tggtttttcg caaaatattc catctgagca gtgacttctg gtgttcgttg
     8161 acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa taagcattag gatgtactgg
     8221 cacttcttca tatacgatgg attgccaatc tttacgtaaa aatgcgcgat agttgatcaa
     8281 gaattcttct acatccttta ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat
     8341 ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt ttcttgattt cattttccaa
     8401 tgcttgataa tccgctatgt aaatcggcat ttctacttgg cgaaattctg cgagccgctg
     8461 agctaatttt atatccggta aatatggctg tttcatccga cgattttggt acaagacaaa
     8521 acagtatttt tgccatagtc cttcaggaaa atcgatattc ttctgcttaa gccataataa
     8581 ttcgtctaag gccagatcaa cttttgtcat tccctgatgg atcgaagctg ccactgtcgg
     8641 agagacatgc aggtgatcgg caaagtggtt gatgcctagt tttaggatct ttccggtttt
     8701 tggcgattta acgatgtatt gcacttttaa ttctctacca cactcacaat acaatttttg
     8761 ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag tcagaaaaca cccaatctgt
     8821 tgcagctaaa taatgttctt ccataaaacg agagctgatt agatatcggc ggtgcttatc
     8881 gactagttcc cgctgttttt tcgttaattt tgcatagatc tcccaacgtt gactgcttgt
     8941 gtattgttta gaaattttga tgggatagct tttgctgatt tcttttggaa accattcttc
     9001 aaagacggat tgccattctt ccacggctgt ttctttcctt tctaattaca aaaagctggc
     9061 tactaacata acgttagcaa ccagccagtg tttcttgcta aattagtgag ttgttccggc
     9121 atttccggaa atatcttctt tattttctac tgctgcttca actgcataag tcaaagaaga
     9181 agcaatgtct tctaaagaca tagaaggcgt acccactgga cgatcgatca cttgatcggg
     9241 tgagaatggc acgtggatga acccacctcg aattgccggg aattgtgttt cgatcaaatg
     9301 aagcaaacga tacatgatat cattgcagac aaatgttcct gctgtataag agatgtacgc
     9361 tggtagacca tgatcttgta cattttttgt cattgctttg attggcaatg aggtaaagta
     9421 agcggctgga ccgttttctt ctaaagccgt atcgaatggc tgatttcctt cattatcagg
     9481 gattcttgct tctgctagat tgattgcgac acgttcgaac gagactgctg accgcccacc
     9541 tgcttgtccg acacagatca cgacatctgg ttggtgctgt tggatggctt ctttgactac
     9601 ttgactgctt ttttcgaata cggtcggtac ttccactttg atgatttcag caccgctgat
     9661 ttcatctggc atttttttta ctgcttcata ggctggattc actttgtccc caccaaatgg
     9721 atcaaaacct gttactagta ctttcatctg ttgttcctcc gtccgattga tttaacatat
     9781 ggttatcttt tatcatacca ttttccttga acagcgtaaa taaaggaaac aatagtttaa
     9841 ataacataac agatcaaact taatatattc ttataaaaaa atagtgttcc actacaatgt
     9901 tagaagaggt tgcgacaatt attctgtcac aacccctctc ttctattatt taaatagaat
     9961 catataagca atttggaaaa ctagcataaa taacgcaata aacaactggt ttttgatcac
    10021 tccgtatttg tctttcatct ccagcatcgc tacaggcacg atattgaagt tagcagccat
    10081 cggtgtcagc agggtcccac aaaaaccgca agtcaatgca accattccta ttaacgctgg
    10141 attcgctcca tgactaaata cgaatggtgc accgatccca accgtcatca cggtgatggc
    10201 cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat
    10261 gccgatgttc acatttcctt ctggaacgat cgtacctact gcattggaaa tcactgtccc
    10321 aactccagcg cttgtaaata ccgcacctag agaagctaaa agcattggca acatgcttaa
    10381 cggccctact gtgcccagca tatctgctgc atcatctaaa aatgtacttg gtcgattatc
    10441 tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa
    10501 agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg
    10561 aataaaaatt ttcattccta gtttgtctga cattttttcc atatattctt ttgatggaag
    10621 ccggctttct cctttgctga ctttcttagc aattgctggt atggtcatcg caaagatcaa
    10681 gccaccgttc acgattgccg gaagccatct tcctcctgca ataactaccc ctaagaccgt
    10741 ccagaaaaag gctgacccat aacgatgagg attggtttta tctgttaaat ttcttactcc
    10801 agtgtaaatc aatacgagtc ccatcacgat ataaaggatc tccagtaatt tatcgcctaa
    10861 taaaatatca ggatttgtaa aaaagtccat cttattgccc ctttcctgga taatatttct
    10921 tcgctaattt cttttcttta agatagaagt agacacttgc cacaactaaa gccgtgatag
    10981 ccactggaac ttccactaaa gcaagctgtc ccaatgttac gtcatatcct agatctttca
    11041 gtgttgattg gactagcaac gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc
    11101 agcagacatt ttccatagct gaagccatac cttttagttc ttcttcatgc ttaggatgga
    11161 cttctaaccc ttttgattcg atggtcccca atgccatcgg taaaatgatt ggacgaacaa
    11221 atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa gatcattcga aagatcccat
    11281 aaatatctac aatcatacca gcagatactt tcttgaatcg cttgatcaag gaagccgctg
    11341 attctttcag accatttctt tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg
    11401 ccatcccgcg attgtttaca aaacttgtac ctaaaagttc taacattcct tgaatgccaa
    11461 gaccgccagt caaagctgta acaactaaag caaccattac gatcaatatc gagtctaatt
    11521 taaatgcaaa cccgagaata acaatcaaga cgcctaaaag gctaagataa ttttccatat
    11581 attttcctcc ataaaatgac aatatttttt taattataaa gcaaaacaca taaaaaatat
    11641 agttgttttc tcacgaaata gacactattt ttaaatattt ttcgttattt ttttatcaca
    11701 aaaattatct tacttattgt aaggtaaagc tttttttaat ttattgcccg ataatat
//
LOCUS       DACCQP010000084        11543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCQP010000084 DACCQP010000000
VERSION     DACCQP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.087"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            346..552
                     /locus_tag="IVO87_002358"
     CDS             346..552
                     /locus_tag="IVO87_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7450517.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(754..1755)
                     /locus_tag="IVO87_002359"
     CDS             complement(754..1755)
                     /locus_tag="IVO87_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7450518.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1760..3673)
                     /locus_tag="IVO87_002360"
     CDS             complement(1760..3673)
                     /locus_tag="IVO87_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7450519.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3841..4347)
                     /locus_tag="IVO87_002361"
     CDS             complement(3841..4347)
                     /locus_tag="IVO87_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7450520.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4507..4947
                     /locus_tag="IVO87_002362"
     CDS             4507..4947
                     /locus_tag="IVO87_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7450521.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4973..6130)
                     /locus_tag="IVO87_002363"
     CDS             complement(4973..6130)
                     /locus_tag="IVO87_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450522.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6133..6489)
                     /locus_tag="IVO87_002364"
     CDS             complement(6133..6489)
                     /locus_tag="IVO87_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450523.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6787..7761)
                     /gene="sgrA"
                     /locus_tag="IVO87_002365"
     CDS             complement(6787..7761)
                     /gene="sgrA"
                     /locus_tag="IVO87_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7450524.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7957..8796)
                     /locus_tag="IVO87_002366"
     CDS             complement(7957..8796)
                     /locus_tag="IVO87_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7450525.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8981..9868
                     /locus_tag="IVO87_002367"
     CDS             8981..9868
                     /locus_tag="IVO87_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7450526.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10462..11157)
                     /locus_tag="IVO87_002368"
     CDS             complement(10462..11157)
                     /locus_tag="IVO87_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7450527.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11141..11539)
                     /locus_tag="IVO87_002369"
     CDS             complement(11141..11539)
                     /locus_tag="IVO87_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7450528.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3437 a         2301 c         1730 g         4075 t
ORIGIN      
        1 actggctttt ggcctcattt taggttatca tttgtaattt tatttcacag ttgatatgat
       61 tgttatgtaa aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg
      121 tatcctaaat cttttctatt atattcccat attttctatt attggtctag acgtatgatg
      181 gtaaactttg tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt
      241 ttcaagttcg ctagatgatc attttattca catcctaaca aacttgtcac atttatagat
      301 tcttaatcta tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat
      361 atttctttgc tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag
      421 ccttatatca ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc
      481 tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa
      541 caacaaatct gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt
      601 cccaaatttc gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa
      661 tattcataaa ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt
      721 gacagattac aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt
      781 ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac
      841 agcatttttc tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga
      901 agttacttct tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt
      961 ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc
     1021 aaagtcctta ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt
     1081 atcacttact tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt
     1141 aatctgcttg ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg
     1201 aagtgctaac aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat
     1261 attcgaccaa tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa
     1321 ttggataact ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat
     1381 gatttgctgc gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc
     1441 tcctacatat gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt
     1501 cggtgtgtag atatccatat tattattttt gtactttgaa ggataggata tttctttttt
     1561 tatatcgatt tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt
     1621 ttcacttgtg tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt
     1681 aatcaaaaat acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg
     1741 cttgaaccat ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc
     1801 taacagataa aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg
     1861 gcaacattct tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt
     1921 gcgtatcatc acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct
     1981 ctgtcggatc ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag
     2041 ttggtatcgt ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc
     2101 cttcgaatgt cttttgctct gtcatataat tttctccata agctccgtaa agctggattt
     2161 cgtcctggtc tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat
     2221 catggaacac tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt
     2281 cctgtgcctt tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata
     2341 actcatataa tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa
     2401 tcaatttagg caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat
     2461 gcatctcctg atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt
     2521 gatccccaat gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct
     2581 ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc
     2641 ctttgacatt gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg
     2701 aaccaccata aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt
     2761 aaaggacgtc atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag
     2821 tcgctctgga atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt
     2881 ctgtaacgat cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat
     2941 ctgaaccttg atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta
     3001 gtacagtttg atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg
     3061 tcccatcatc tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat
     3121 cgtacaatag ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat
     3181 taacagcaag ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc
     3241 cattaagtat ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat
     3301 tttcaccttt accgatatca tcattttctg tcccattctc tttgtcactt actttagcag
     3361 aagaggattc gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg
     3421 tggtagcggt cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat
     3481 ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg
     3541 tttctacctc ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg
     3601 ggacgcttgt acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc
     3661 tcaagctatt cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt
     3721 tatacaaaaa ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac
     3781 gtaataaagc agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac
     3841 ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt
     3901 ttcctttgct ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag
     3961 ctggtatcct aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc
     4021 cccacataat tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt
     4081 ataaaatgaa ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc
     4141 cgacaatagc tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa
     4201 aataactggt ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac
     4261 tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac
     4321 tactattgcg tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat
     4381 agtatttctt atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct
     4441 aaatggcact tttcgactat ttcttcatgt tattatttca taaatactta gctattggag
     4501 gaatgtatga tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa
     4561 cgttggctta tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta
     4621 accaaaaatg aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc
     4681 tctactcatt ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt
     4741 gaactatata ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac
     4801 caatctgcaa aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa
     4861 actaaaagaa agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa
     4921 gaaaataatc tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa
     4981 cgaaatccaa tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag
     5041 ctttcagacg cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat
     5101 tcgtttgatc atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact
     5161 gatcagcgga tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg
     5221 cccataattc ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt
     5281 gttttataaa aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt
     5341 cataataagt attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga
     5401 ttggatacat cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag
     5461 catccacctt cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga
     5521 catatagata atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt
     5581 ctacaataga cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca
     5641 aatcttcttc caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg
     5701 ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat
     5761 gcgtaaactc ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat
     5821 acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt
     5881 tctttctatc tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac
     5941 gtttattttt tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca
     6001 tcaagtgaaa aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca
     6061 agctgcaaga taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa
     6121 tcgtacgcaa acttatataa ttttttgact aagattttct aaggattgat aagtaccttt
     6181 gtaaagtgaa tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt
     6241 actcagtaaa aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga
     6301 taatttatat tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat
     6361 cttagccagg gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc
     6421 tctttcataa caatagttca aaaattgata tacttgcctt tcattgatat caataaattc
     6481 ttcaaacaaa gtacatactc ctttttatct attatttaga cttattccga cagctacatg
     6541 aataacgtac aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca
     6601 ttgtcaatta ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa
     6661 tatgttagta aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact
     6721 ctcgaatgaa atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt
     6781 tattctttat ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat
     6841 gagtaacaca cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa
     6901 atcgcttttt ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac
     6961 aaaatcaggc tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt
     7021 agttggtatg ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct
     7081 gctcgtgctt cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt
     7141 actctccgta ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc
     7201 cgtactgctt tcgctgctcg tgttttctgt gctactttca ctgctcgtac tctctgtact
     7261 gctttcaccg ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg
     7321 cggtccaaca actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc
     7381 ataaaaaaca atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc
     7441 agaaatcatt attgtatact caataccagc ggtccattgt acgtctttta aataaacttc
     7501 cccaataact ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac
     7561 atcactagca ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat
     7621 gtcataaaca atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt
     7681 tgcagaagta ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa
     7741 tacggtcgct gttttcttca tttctatcac tctccatata attaccttct atttgataaa
     7801 ggaattatat aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa
     7861 gatgtcgtgt attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa
     7921 aagcaagacc gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt
     7981 cttcatccgc tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa
     8041 taatgtagtt gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg
     8101 cgattggatc agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa
     8161 aatagctcct tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc
     8221 tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag
     8281 aaatgtcatg ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat
     8341 tgctattaac ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc
     8401 ttctgatgcc cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc
     8461 gtttcctgaa tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa
     8521 cactccaatt ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga
     8581 aacaccataa tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa
     8641 aaaaacagct aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat
     8701 aaacatatcc gcaagtgcat tattgacaag aattcctaaa acaaaaccaa ctaagactat
     8761 taaaacagat ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag
     8821 tttaacaacc cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag
     8881 agaatagatt tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc
     8941 tccctaattc ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa
     9001 acaactacta attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa
     9061 gtccaatgaa acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga
     9121 taacccaaaa gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc
     9181 atttgatgat gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga
     9241 acaaatcaaa aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga
     9301 agagacatat aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa
     9361 tgtaaaatta acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag
     9421 tacacagatc attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa
     9481 tgaaggagaa aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga
     9541 aactgatgga aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa
     9601 agcagcattt aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac
     9661 gacttatcag caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa
     9721 taaagacaaa tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac
     9781 ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga
     9841 tccttatgta aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa
     9901 tttgagaagt gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa
     9961 cttcatcggg gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg
    10021 aggatataat ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag
    10081 attctttcat gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa
    10141 actcaaactc gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt
    10201 tcttcttatt ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt
    10261 tgtttttatg aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag
    10321 gttagggaat acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact
    10381 ttaaattaat gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt
    10441 ttattttatg gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt
    10501 ttctgcttgg aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga
    10561 gggcatctct aatacttgat ctagtagctc tttacctaac gttatcgcat gagctccaga
    10621 tcgaatggct gtagtgactt gttcacaatt tttgaaacta gctgctaata tttttgttgg
    10681 tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc actttcatag catcaatgcc
    10741 ttgattttgc atcctgttat aataaggtgc aatataatct gctccattat taatagctaa
    10801 atacccttgt aattctgtgt aaatagctgt accggtaatc aaataacctt cttttttcaa
    10861 ctttttgatc gcttgaaggc ctgcttgatt caccggcacc ttgataaacg tatcttttcc
    10921 cagttcttct atgataactc gagcatcctc tatcatttct tcttccgttc ttcctattgt
    10981 ctgaatatgg ataggaatct gtccgattat ttttttgatt tctttcatat gcgagaagaa
    11041 gtttgacgct ccttcttttt tcagaattgt aggatttgtt gttacgcctg ttaaattcaa
    11101 aaactgttga caagaataaa tactttttgt atttgctgta tcaagcatga tttccatata
    11161 gctttccccc attctgatcc tcttgatcga ttttaatgat tgtccccttt cttaaatgcg
    11221 gcatttcctt atcttctaca tacattgttc ctggaagttc cggctcatga gatccatcaa
    11281 attttatcgt gatatgtcct aattcattca aattagtttg gacaacatct cctactgctg
    11341 tgattacata catttgttta tcaaaacaga gcttcatatc acaagttatt tcttttctta
    11401 cctgattcaa ttcaatctta taacaataat ctgttaatgt ttccggtgct tcatctccaa
    11461 ataaaatcat tattttttct ttcctgaata cttctgcttc tataccaaca tctgttacaa
    11521 ccgtttcaaa gatcgtcatc tct
//
LOCUS       DACCQP010000085        11433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCQP010000085 DACCQP010000000
VERSION     DACCQP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.088"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(24..359)
                     /locus_tag="IVO87_002370"
     CDS             complement(24..359)
                     /locus_tag="IVO87_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7450529.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            645..2525
                     /locus_tag="IVO87_002371"
     CDS             645..2525
                     /locus_tag="IVO87_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7450530.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2604..2831
                     /locus_tag="IVO87_002372"
     CDS             2604..2831
                     /locus_tag="IVO87_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7450531.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2836..3378
                     /locus_tag="IVO87_002373"
     CDS             2836..3378
                     /locus_tag="IVO87_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7450532.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3629..4306
                     /locus_tag="IVO87_002374"
     CDS             3629..4306
                     /locus_tag="IVO87_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7450533.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4627..5868
                     /gene="sstT"
                     /locus_tag="IVO87_002375"
     CDS             4627..5868
                     /gene="sstT"
                     /locus_tag="IVO87_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7450534.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6015..6650
                     /locus_tag="IVO87_002376"
     CDS             6015..6650
                     /locus_tag="IVO87_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7450535.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6841..7584
                     /gene="truA"
                     /locus_tag="IVO87_002377"
     CDS             6841..7584
                     /gene="truA"
                     /locus_tag="IVO87_002377"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7450536.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7818..8651
                     /gene="msrA"
                     /locus_tag="IVO87_002378"
     CDS             7818..8651
                     /gene="msrA"
                     /locus_tag="IVO87_002378"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7450537.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKKIRKNHF
                     D"
     gene            8732..9725
                     /locus_tag="IVO87_002379"
                     /pseudo
     CDS             8732..9725
                     /locus_tag="IVO87_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10014..10712
                     /locus_tag="IVO87_002380"
     CDS             10014..10712
                     /locus_tag="IVO87_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7450538.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10862..11317
                     /locus_tag="IVO87_002381"
                     /pseudo
     CDS             10862..11317
                     /locus_tag="IVO87_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT         3776 a         1849 c         2385 g         3423 t
ORIGIN      
        1 atactcgttt acataaaaaa agattattta gatgcgataa tatcatagat aaagaaacat
       61 tcatcataag aaacagtgat cccaaattct gtttcaatat ttttgaaggc cttttttaca
      121 tgaaaaattg tttcatgaga aggtcttgtg tcatcttctg atataatttt aagttcatga
      181 ccgtttatca atgtacgctc aattagacac gctgtatgaa tcaaaagttt taaaagaatg
      241 gaatgagtga attttttgtg gaaagtacat tccaattctt ggataaaatc cattaagctt
      301 ttttggatct tattcccagc taataagtag agatatttgt ccactgcttc aactcccata
      361 tcaaaaaacg atatttcttg tgaatgagtg gctgctgccc ttttttgatt gttgttcttc
      421 agtcgtaaga tgacaaagca tcttatcttt gaataaaaaa atagaactac ctaaggaagc
      481 gtttcggcgc ttcctttttc tatatgatcg tttaatgact gtaggaattc agtataccaa
      541 aagaaaaccg cctttttgaa taaacttgat agtggtcaat tttttatgat ggataagtat
      601 ctatacttag tctcgtaaga atgaaaagga aaagagggag aaagatgaaa ttacaaaagt
      661 ggatccaaga gcatcgaaat catatcaccg ccttatcagc tgtgttgatt atttttggtt
      721 ttatcgggaa atttactgga tttgtcttgt tctataacat tgctctagcc atagcttcta
      781 ttattgcggt catacctatt ataatccatg cgtatcaggc agtaaaagtc aaagttatct
      841 caattgattt actcgttact attgctgtgc ttggtgcctt tatgatcgga gaatacaagg
      901 agtcagctat cgttacattc ttgtttttgt ttggtagtta tctggaacaa aaaacattga
      961 ccaaaacgcg ggaatcaatc aaggctttga ccgagatggc cccatcgact gccagcgttg
     1021 tccaagaaga tggaacgacc gaagaagtgg aaatcgatga cgtggaagaa ggcgatatcg
     1081 tcttagtaaa aactggtgga gctattcctg tggacggacg agtcattgac gggaaagggt
     1141 atgcggatga agccgcagtg acgggtgaat cacgacaagt cgtcaaaatc agtggagata
     1201 acgtattttc tggaacgatt ttagcagatg gctacgtgac gatcgaagca actaaggtcg
     1261 gagatgatac gacctttgcc aaaattatag aattagtcga agaagcacaa gataccaaat
     1321 cacatgcgga aaaattcatt gatcaatttt ctcagtatta tactccaacc gttttactta
     1381 ttgcgttgct tgttggtctg atcagtcgtg attttcgttt agccatcact gttttggttc
     1441 ttggttgtcc tggcgcttta gtcattggtg cgcctgtttc gaatgtagca ggaatcggta
     1501 atggagcgaa acggggcatc ttagtcaaag gcggtgaagc aatggatact ttttccaaaa
     1561 tcgatacttt tgtctttgat aaaaccggta cattgaccaa aggaactact gctgtatcca
     1621 ctatcaagaa ctatacagag gatgaagggg acagcttagc tttagtggct cgattagaaa
     1681 cgttatccga tcatcctctg gggcgcgccg tgatccatta tgctcaggag aaagacattg
     1741 attttcaaca attagcagtc aaagataatc agacgatcaa gggacaagga atcacagctg
     1801 agattgatgg tcatacagtt tgtgcgggca acaccaaatt gattgccgct cacaaacttt
     1861 ctctcacacc aaagcaagta caagatttac atcaattaca acaaagaggc agctcagtca
     1921 ttattgtagc aatcgacaaa aaaatcactc aaatcattgg tgtgtcagat gtgattcgac
     1981 cagaagtcgc tgaacaatta gctatgttga aacaagcagg agcaaaacat ttagtgatgc
     2041 tgacaggtga taatcagatg actgctgatt atgtagctga catgttaggc attgatgaag
     2101 tccatgctga attattgcca gatgaaaagg ttcaatttgt gaaaaaatac caagaacaag
     2161 gattgcacgt tgctttcatt ggagatggaa tcaatgattc tccctctttg gctgcggctg
     2221 atatcgggat tgctatggga tcaggaacgg atgtcgctat cgaaacgagt gacgttgtct
     2281 tgatgcaatc tagttttgga tcgttggttc atgcttaccg tttggctaag aaaactgttt
     2341 tgaatacccg tgaaaatgta gtgattgcca ttggtgtcgt cttattttta ctcatcggct
     2401 tatttgccgg gtttatttac atggctagtg ggatgttcgt tcacgaagct agtatcttga
     2461 tcgttatctt taacgcgatg cgtttattgt attttggaca cgaaagcaaa gaagaaaaat
     2521 cgtaaaactt gataagaatc aattttttta tggaagctgt aatctagaat aagactatca
     2581 agaaaaagga aggaagaact acgatgaaaa aaatgattat gcaattagat acactagctt
     2641 gtccaagctg tatgcaaaaa atagaacatg cggtttctca acagcctggg gtaagcaata
     2701 cgaaagtttt gtttaatgcg agcaaaatca aagcaaactt tgatccagat gaagtccacc
     2761 ctgaacaatt agctgcagtt gtggaaaaat taggttatga agtccaatca acaaaagtaa
     2821 aagaggcgta aatcagtgaa tatagtggaa gaaaaatttg ctcaagaaac aaaacaagca
     2881 gatatcgatc atcataaacc aactgctggc gccatggtgg ggcatgtatt ggcaaatttt
     2941 aaattagaag aaatgaaatt gatccaagca caattgtatg ttttgggtac atcttcgcaa
     3001 gaattaagta cccttttttc agaatatgct gatcaagaag cgagatggtt caataaaatc
     3061 tctcatgagt tagtgattga aaatgaaatc gttccgtcta ctttagaaga agtcatgcgt
     3121 tatggaaaaa tttacgaaca tggtcaaaac aaatatttac cagcctcaac aatgttggaa
     3181 aactttgcaa aagattttga tttccaaaat gtctttatca cacgggcaat caaattagct
     3241 gaaaaagaag aaaaatatgg cttgaaacaa ttgcttgttg aacttttaag tttcaataaa
     3301 gaaatcatct accaagtaca aagactccaa ggaaaaactg tccgacaaga tttggatgag
     3361 gaagacgatg atgactaaaa gtctatgttt cttcaaatac aaatataaag cgtgaggtaa
     3421 attggaaaat cgcgaagatt aaaataaaag aagcacccgc tgatgtatta cagacaacat
     3481 cagcgggtgc ttcttagttt ttaaaaggtt taaaagatgg atgagaaaat acaaaaaatc
     3541 aataaaatca cattgcttat ttgatagaat aaagaagtgg gagtaatcga aaggagtttt
     3601 ttaatttaaa aaaaggattt tagtaacaat tggtctagct tttttgttag ctggttgtac
     3661 caacgggaat gaatcgaaaa aagaatcaac aaaagaaaca gcgaaagcaa caattgcaac
     3721 tactacaaca actagcacag agagcaagcc aactgcagca agtacggaaa cagcagatac
     3781 ctctacttcg tcagagtctt ccaaaacgac tgaaacaact caaacatcta cttcaagttc
     3841 ttctgtagag gcttcatctt cagaaagcag cgaacaagtc gaacaaacac tgtggaatgc
     3901 agacaaatcc gcacaattag aggcatttat ggctacatgg ggccaaacaa tgggacaaca
     3961 atacaaaagc tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagt
     4021 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     4081 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     4141 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     4201 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttta acgagacagc
     4261 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat gggtaagtaa accagtgaga
     4321 gtgagagaaa gaagagactc agtggaaaat attaattgat tgctatgtct aagaattaag
     4381 atgaaggatg aaatttctag tttcgtcctt catcttagtt acgttttact tggataacta
     4441 taataataac gataaatagt tttctctcga cttctttaga gattgaataa accctccaat
     4501 actaaaattg aatgttgcaa gagcatgaat tgccaattgt attattagaa tttttagaaa
     4561 attgcacctt tagtttcttt agataaaatg atacaatgat tgttaaaatt gtgaaagggt
     4621 ggtcatttgt tagagaagtt tttggatatg tcgttagttc aacgtattgt tgttggtatt
     4681 ttagtcggaa tattgtttgg tatctttttc cccacgtgga catttatttc aatattgggg
     4741 acgttgtttg tcagttgctt gaaggcaatc gcacctattc tggttttttt cttgacgatg
     4801 gcgtcaattg ccaagcataa aattgggaat aagacattcg tgaagcctat tttgatctta
     4861 tatttagtgg gaacattgct ttctgcactt gtagcagtca ttgtaagttc aatttttact
     4921 gttcctatta ctttacaaga aacagtatct gaaaaagcac cgcaaagttt ggaaagtgtt
     4981 ttatctacta tgttgacgaa tgtcacacaa aatcctgttc aatctcttat tgatgcgaat
     5041 taccttggcg ttttattctg ggctgtattg ttgggactag cttttcgagc acgttcagaa
     5101 ccgacaaaag agttggtgga tcagatatct atagcgttgt cacaagtcgt acaagtgatt
     5161 attgcatttg ctccgatagg gattttagga ttggtttatc aatcaattgc tacgactgga
     5221 atcgctggat tggcagagta tctgcagctt ttacttgttt tgatcggtac gatgctgttt
     5281 gtcgcattag ttgtgtaccc attcctcacg tttttgttta ttaaagaaaa tccctatcca
     5341 ttgattttct tttgtttgaa agaaagcgca cttcccgctt tctttacccg aagttctgcc
     5401 gcaaatatcc cgataaacat gatgttggct gaaagattga aattgacaaa agagtcttat
     5461 tccatatcga tcccgttagg tgctacgatc aatatgggcg gtgccgcgat taccatttcc
     5521 ttgatgacgt tgactgctgt acattcttta gggattgaag caccatttgt tttgaaagtc
     5581 cttttatgta ttttgtctgc tttagctgct tgtggagctt caggtgttgc gggcggttca
     5641 ctgttattga ttccattagc atgtagtttg tttggtatct ctaatgatgt cgcgatgcaa
     5701 atcgttggta ttggatttat tatcggtgtt atccaggatt caatggaaac tgccttaaat
     5761 tcgtcaagtg atctgttgtt tactgcaata ggagagctag gcgcgcgtaa aagaagtggg
     5821 gaaaaaatca aattgaaaga ttgtttagat ggattaagtg aacgttgaac gttagatatt
     5881 tagtattttt aaaatgattc gagtttttaa tgagttatat agatgttcgt gtaaatagag
     5941 gtttctgtta aatcaagata aagtcgttta taataacaat taaaagcata ggtaattgtt
     6001 attggaggcg tatcatggat tttttacgta aatttggttt tttcttttta ttattaggcg
     6061 tgctgagtga ttttttgact ccgtacgtat taggattgta ttatcctgaa ttagatcaaa
     6121 tgaagagcgt gatcagtgta tttggagagg tttccagtcc tgtaagaaaa gcgtttctta
     6181 tttggtctgt ggtttcaggg atctttttca tcctgtcatt gcctgcattg tatgctgcat
     6241 ttgtaagtac atctcgtgtg ttagctaccc tagttacagc agccatcgcg ttctatggta
     6301 taggtgattg tatctttaca ggtttgttca gtatcaatac ccatgaatct tcgtggaatg
     6361 tctcgacatg ggtccataat atcggatcag gtttaggtta cagtggtttt ttattgtttc
     6421 cagttcttct tttcttgatc tatcgaaaaa atgggcaaat ggacgtaagc cacttatacc
     6481 ttggactgtc tgtgatcagt ttgctttttg cggcgattta tggactggca agaattcctt
     6541 ctataaattc ttggccatta ctgaataaaa taggtttttg ccaaagaatc agttttttct
     6601 ttaattatgt gcccattgcc tggttgagta tcttgcatct caaacagtaa aacttgagcc
     6661 gtcaaattga acagtcagcg gatacttcat ccggtggctg ttttttgttt ttctgaaata
     6721 tatatttcca cagttttctt gtatagcttc tgtcttagag aagcaaggag ctatattttg
     6781 tgctacaatc ttaaaaaggt agagagaaga ccaaatggaa gacagaggag ccactaaaat
     6841 atgagaaaca tcaagttgac aatcgaatat gacggaaaaa gatattcagg atggcaaaga
     6901 cttggagatg acgataaaac cattcaagga aaaatcgaaa aagtattgca tcagatgaca
     6961 aatgaagaga tagaaattat tggatcagga agaacagatg ctggcaccca tgcgcgagga
     7021 caagtagcaa attttaaaac aaacacagaa attggactgg cagaaatgat cgactttatg
     7081 aatcgttacc ttccgcggga tattgtcatc aaaagaatcg aagaaatgcc tgagcgcttc
     7141 cattcaagat ataatgcggt gggcaaaaaa tacagctatt atgtttggaa caacgtgatt
     7201 ccttcagcat ttgagcgaaa ccacagcttt tatttccctc aagagttgga tatggacaag
     7261 ctgaatgcag cttgcgaaaa attagtgggt acacatgatt tcattggttt ttctgcattg
     7321 aaaaaatcaa aaaaatccac agtgcgtacg attgatgaat tatctatcga acgaaaggga
     7381 gaaatgcttc attttacttt tgtcggagac ggattcttat ataaaatggt gcggattatc
     7441 atggggacat tactagaaat cggcacaggt gccatgccga tagaaacaat cgatgaaata
     7501 tttgaaagca aagtgcggca acatgcagga gaaaccgtcc ctgcacaagg tctgtttttg
     7561 gatgaagttt attatcgctc ataaaaaaga atcatcttta ctcgacttct gagaattgga
     7621 gaacatccct tttttcagag gtcgtttttt agttaaacca aaccatagag tatgcttttt
     7681 aagataaaga tcatcttata tcatctgaag ctgcttatct tctttgaata aatacgattc
     7741 aatcgattat ttcctcactt tatgataaat tacagctaaa gtcactagct tgtcttcttc
     7801 ggaaaggagg agaatctatg gatgaggaac aaatattcat tcaaacactt tataatctga
     7861 ttttgaatcc gaatacacga gattgggaac gaaaagtatt gattcagaca aagaatgaca
     7921 caagagaaaa tatatccgtt aaagaacagt tatcaaaact agaagtaaca ctacgtccat
     7981 tagctatccg aatgaatctg acgcctgatg taatggactt ttatttattg attacggaag
     8041 gatttgataa ggagcaaaaa tatgactttt ccaagcatgc catgcaagat gctgactatc
     8101 aagaacgtgc agtatttgca gggggatgtt tctggtgtat ggtcgagccg tttgaatcga
     8161 aaaaagggat cctatctgtt ttgtcaggat acacgggcgg acatgttgaa aaaccgaatt
     8221 atgatcaagt aagtggtggt tacacaggtc atgtggaagc tgtggagatc atttatgaca
     8281 caagagaaat aagttatagc gagttgctga caatctattg gcaaattact gatcccacag
     8341 atacatttgg acaatttcaa gatagaggga agcaatatcg tccagtgatt ttttatcaag
     8401 acgaacgaca aaaagaatta gcggaacaat caaaacagaa actagacagt tcaggaactt
     8461 tccaccaacc aatcgtcaca aaaatcgaac cagccggtac gttttggcca gcagaaaact
     8521 atcatcagca attttacaaa aaacaaccga aacgctataa aaaaatccag caagcacgta
     8581 atcaatttct gatctatcaa cgagtaaaaa acaaatggca aaaaaaaata cgaaaaaatc
     8641 attttgatta actccttttc tttaaaatgt agtaaactaa aaatagaaaa caagaaagcg
     8701 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
     8761 aaaaatggaa tatcgtaaat ccggtcattc aggtttaaaa ttaccgattt tatctctcct
     8821 ctcggattat ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta
     8881 agaggcgcat tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct
     8941 gctggagctg cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt
     9001 gatgaactga ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa
     9061 tggggctctc gtaaatccat catatcaagt tgtgaccaaa gtttccaaag aatggggcta
     9121 gattatgttg acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca
     9181 gcagaagcgt tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac
     9241 tataatggag aagatacaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca
     9301 ttattcatca gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc
     9361 cagtattaga agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc
     9421 tgacaaatcg ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac
     9481 cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa
     9541 tcgccgtatc acgaggacaa tcattagcgc aaatagccct tgcatggaat ttacgacaaa
     9601 aatctgttac aagtgtactg atcggagcaa gtcggttaag tcagttacaa gaaagtgttc
     9661 gaatgatgga taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag
     9721 aatagaaacg atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc
     9781 aagaatcgaa tgaagatcgt ctgttattga acatcatatt ttttgtcgat caaaataaaa
     9841 atgatcgaag gctaggagtg aattttatcc tagtcttttt tatggtaaat atcttcttcg
     9901 tttgtatgtc tatgaacgga ttaatcgatt ttttatacaa aaaatcatca gatgattcaa
     9961 acgtgttata ataattacga aaaaatacat cagatgaaaa ggaggtatgt ggtttggaag
    10021 aaaataaaac cctagtagaa gtaactactg acaagatcct tagatattta aaggaaatgc
    10081 agttgcaagt aggagataaa ctgccgaatg aatatgaatt gtccgattat ttagaggtgg
    10141 ggcgaagcac ggtgcgagaa gctgtgcggg ctctagcttc caggaatatc cttgaagttc
    10201 ggcaaggagc ggggacattt gtcagtgata agcgagggat tgccaatgac ccactcggtt
    10261 tttctttgat tgaagatcag gatcggatga tacgagactt atttgaactg agatatttat
    10321 tggagcctaa gatggcaggt ttggcagcgg aatatgcaac gaagaatcaa atcgtccaaa
    10381 tgaaaaaatt aacgcaaaag attgaaactt cttttcgaga aggcacacaa gaacatgttc
    10441 aattagacat cgattttcat acgatgattg cagaagctag cggaaacata gccttgctgc
    10501 atattatccc aatcattaat gaatcgattg ctttgtttaa taaaaattat gatatccagc
    10561 atctgaaaat cgagacgatc gatattcata cagagatcgt tcaggccatc gaaaggcgga
    10621 acgtacttgc tgcgtttgac gcaatgactg tccatatggc aataaatcgg agagaattac
    10681 agaaaataat aggagaaaaa gaaactaatt gacagttcat ctgatgtatt ttatgataaa
    10741 tacatcagat gattttttgt tttatatttg gtaaaaaaga ggagtaaata gtttctgttc
    10801 accaaaatgt aagcgctgac taatagacaa aaagagatga cgtcaaggag aagaagcagg
    10861 aatgagggga tctggaatga gttttatgga taaattagcc aaagttttag ggaacgtggc
    10921 aacgagaatc aataatttaa ggtatatcat ggtgataaaa aacgcatttg ctgcattgat
    10981 tccggtaatt attacgggtg cttttggcac attgttttca gcaatggtct ttgactcgga
    11041 aaatggctta gcacagataa gttacttgcg ttttttagaa gaattaaagc ctatcgcatc
    11101 tgccatcagc tatgtcacac taagtttctt gaccatctat gctgtttttc taatagggat
    11161 cgaattagcc aagctgaata aagtggatgg tgtattccct ggtatcatcg cagtgatgtc
    11221 ttatttatct gttaatccaa ctgtttatga attagtgaat gaaaatcaga ctgttatagt
    11281 agaaaacgta ttggctaaac aatatacaga cacgaaagtg gtgttagtat caaatcttag
    11341 acagcttttc gtagagacaa gaataaatta aacttatcta cgagaggatg aatttttatg
    11401 acccgatatg aagaaaattt taaacaaatg att
//
LOCUS       DACCQP010000086        11253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCQP010000086 DACCQP010000000
VERSION     DACCQP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.089"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(83..967)
                     /locus_tag="IVO87_002382"
     CDS             complement(83..967)
                     /locus_tag="IVO87_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7450539.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1062..1499)
                     /locus_tag="IVO87_002383"
     CDS             complement(1062..1499)
                     /locus_tag="IVO87_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450540.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(1716..2627)
                     /locus_tag="IVO87_002384"
     CDS             complement(1716..2627)
                     /locus_tag="IVO87_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7450541.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3084..3512)
                     /locus_tag="IVO87_002385"
     CDS             complement(3084..3512)
                     /locus_tag="IVO87_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450542.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(3540..4178)
                     /locus_tag="IVO87_002386"
     CDS             complement(3540..4178)
                     /locus_tag="IVO87_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450543.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(4182..6218)
                     /locus_tag="IVO87_002387"
     CDS             complement(4182..6218)
                     /locus_tag="IVO87_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7450544.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6344..7009
                     /locus_tag="IVO87_002388"
     CDS             6344..7009
                     /locus_tag="IVO87_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450545.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(8944..>11253)
                     /locus_tag="IVO87_002389"
     CDS             complement(8944..>11253)
                     /locus_tag="IVO87_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7450546.1"
                     /translation="IAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVD
                     RKDLDNQTTTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVII
                     TTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPK
                     STWFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHEN
                     TVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQK
                     VIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFP
                     NQLIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADI
                     ERYNGDINNRLARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLI
                     QAFSRTNRTYPDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKR
                     FKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPT
                     LNNQIQLLEEQIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQL
                     LGTYAANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYF
                     TQASDDLIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEA
                     KPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT         3484 a         2160 c         1732 g         3877 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa
       61 ctcctcggtc acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga
      121 ttgtcacagg taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat
      181 cagctaatga cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat
      241 ctactccttt taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta
      301 aataaaccaa atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac
      361 cgccgactga gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc
      421 catcaacttc tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca
      481 tgaaataagc tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt
      541 cagggataga gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat
      601 ccaatggctt gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta
      661 tccctaatgc tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc
      721 gatgttcttg ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga
      781 caaacacact atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg
      841 ctttttttgt atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta
      901 attgctcaaa ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt
      961 ttttcatcac tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa
     1021 aaaattaggc tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa
     1081 aacatccata ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat
     1141 gataggcatt gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat
     1201 agcaagagaa tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga
     1261 ggaataccaa atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga
     1321 ataataaatg cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga
     1381 tgaatatatt ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa
     1441 acaaatccgt atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt
     1501 tatctacttc tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa
     1561 tatctagggt cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa
     1621 gtagtaatta aactactgtt gatttagttc taataaaaag atttagatga acagttttat
     1681 tgatttaaat agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa
     1741 tctgaagtga atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg
     1801 tctttcaata cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct
     1861 gttgcaattt ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt
     1921 ttcaccattt taaccacata atattccgta gtataagttg aagcattggt agaagtgatc
     1981 acatcagaaa cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc
     2041 gttgtagtag tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt
     2101 gacttgtctt tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct
     2161 tttgcatcat cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat
     2221 tcccaagccg tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga
     2281 aaggctagac tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc
     2341 aattgtgaat caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt
     2401 tctttggttg atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt
     2461 tgcacagcgg aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg
     2521 attgtaccgc ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat
     2581 aagccagtgc ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc
     2641 tacttttcgt tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga
     2701 acacctttac taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa
     2761 gttcgctcag cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg
     2821 ttaccgtttt tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa
     2881 tgttatcgat tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg
     2941 ttcgattttc cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa
     3001 atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct
     3061 attttatata aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat
     3121 gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct
     3181 gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag
     3241 tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca
     3301 gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata
     3361 atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca
     3421 ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc
     3481 cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt
     3541 taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata
     3601 actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt
     3661 ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact
     3721 ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc
     3781 ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca
     3841 tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc
     3901 aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc
     3961 ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca
     4021 ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc
     4081 acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg
     4141 atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc
     4201 aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg
     4261 aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag
     4321 attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca
     4381 aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat
     4441 atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt
     4501 atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac
     4561 ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt
     4621 tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata
     4681 tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg
     4741 atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat
     4801 ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt
     4861 aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt
     4921 atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt
     4981 atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact
     5041 aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa
     5101 cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga
     5161 tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa
     5221 aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca
     5281 aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca
     5341 actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc
     5401 tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct
     5461 gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt
     5521 aatgaaaagt cattattcaa caataaactg atttctttat tcatcaataa gaaaaaggat
     5581 gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata attatatacg
     5641 tctgtatttg aaataaccga atacgaatag ctattttcaa agtcttcaaa aatattagtg
     5701 gcataatcag tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat gacccgttca
     5761 taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt tattttgatc
     5821 gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt ctcgtgagaa
     5881 gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat cttgaagttc
     5941 caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga aagctcagtt
     6001 tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg cccaataaaa
     6061 tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc atttgtccga
     6121 tagagtacct gtttatttat actaaccaag gcaaaaccac tcatgatacc ggtcactatg
     6181 attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta cttaaatgat
     6241 tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaagt gtataggtca
     6301 tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca ccggagaaaa
     6361 aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg aaaaaattgg
     6421 tgtttccgat aaaacgattt ctagttggga aaattctcgt acaatgccag actttgaaat
     6481 gctgtcactt ttacatcata ctttaggtct gccgattgat tttccttcta ttgaatcagc
     6541 taccgaatca atagaaccaa cattgccaga tgaacctctt gttaacaaga aatcttattc
     6601 atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg tcttttgttt
     6661 ggtctatctt tttcttatca attatcaata caaaaatgaa acgatcgatc gattcaatcc
     6721 ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg acaattccat
     6781 cgaacgttgg gtatgtaatg atatctttgg ccatggtaca tggctgtctt ttcacgctgg
     6841 agaggtagaa aaagagaaaa accatgctgt aatcaaacac aaaggaaaac acgtcatgaa
     6901 cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc tcagccaaga
     6961 ctatgacgac gccttaaaaa gaagccctca ggagccagat aggcattaag taggagatga
     7021 acgtagagat agaaaaaatc aatgcacatg atcttatcca aaaaacaggg gtacaaatta
     7081 attgaaaaag ccttgagatg aaagttccta aataaaaaag ttcagaatga taggtatttc
     7141 tggcttatag tataatattt acgccacagc ctcccaatga ttctttttgg aatcataatc
     7201 aaggatggac attcacaaaa atattttgct cattcgatcc gaatagatta acgaccaatc
     7261 tagtaagtaa tatagattgg tacgtagtca agctgcacat aatttagatc aattttagtt
     7321 cttttttata tttctatgat ctgctcattt acttttatat gatggcttac agtaacataa
     7381 ataaatatga taatcgctgg tagtttatcc aagagtactc aataaaatac aaaatggatt
     7441 gttcaagcgc ctatcataat gataaaatga aacaaataaa cgatacaagg aggaagagac
     7501 tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt aatctatggg
     7561 atttcggtag catgggcatt tttttataga ggaatcacac caacctggcc gttttatctc
     7621 ttgttagtgg gaatattagt aggaaatttt gcccctcttc actatcgtac gaactttttt
     7681 ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa cattattgaa
     7741 atgacaatta cctgtataat cattttctta gttatattcc taaacgtctt ctaataagaa
     7801 tctcttaaaa ttagcttgtt acagttagtt acctagttta tataacacag ttatcttatc
     7861 ttccttatca acatcttttg ccaactcttt gtcttaaatt cgaaacatat aagcagagct
     7921 acgaacacca agactgtttc ggctctgttt tttgcatcaa attcactctt atatagctga
     7981 taatctcggc tctttaaaag aaattatttt cgcttcattt tattataaaa aggctaggaa
     8041 ccttgatgtt atcaggttcc tagcctttat ttaagtatta ccccacagac ccttccatat
     8101 agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact taccatttaa
     8161 tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca aaatctggtc
     8221 aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc taaatcattt
     8281 tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa
     8341 ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat
     8401 ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa
     8461 tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg agattacctg
     8521 aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca aatactctcc
     8581 tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa accttgtcat
     8641 tatagcaaaa aatcacttta tatccgtttc atttcagata catttcactt aagcggattc
     8701 caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg gttcctctca
     8761 aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga ggaaacatta
     8821 ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga catctatccc
     8881 tattttctac acttccactt gactactatc tttgaatcgt acaattttct taactcttct
     8941 cgtttacctc aattcattct caagtggaat caactcatca tctaacattt tacgccatgc
     9001 tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg ctttatatcc
     9061 ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt cacctggtag
     9121 atattgcaga ttagaagcat gtaattcttt ctcagaaaca aaccactcat tggaaaagac
     9181 atgaatcaaa tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc
     9241 attactatct aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa cttctttgac
     9301 tccttctgtt ttattcaatt tagccaaagc tttttcaatt tcttctcgtg catctgaact
     9361 attcgcagca taagttccta gtaattgatc aatataagtt gaatcaatat catacaactt
     9421 agtggtattt tcactataaa attcgatcgt tgaaaaatct tctccttctt tttctggttc
     9481 ttcctctatt aatgaaccct tcacggtctc gtacacgcca atttgttctt ccaaaagttg
     9541 aatttgattg ttcaaagttg gagatttcac catgtcatca ttgtaatcgt cataagtcac
     9601 taacgattca taagcacgat tcaattcttg aaatgctttg acgtactcga tacggatttc
     9661 aagtggcgta tgttcatcaa tggattccat ggatgaaacc tcttttaact taccatatgc
     9721 ttgtttgaat ctttttttgg attcctgata acttggataa atcaaacctg attcttcttt
     9781 tgcttttgaa tacagttttg tcgcatcctc gacattcttc tccattgtcg ctggcttacg
     9841 aaacgtcaca atcaatcctt ttgtcttatc aggatacgta cgattcgtac gtgaaaaagc
     9901 ttggatcaat cctgcatact ctagattacg atccacaaat agggtttgaa tcgtaggggc
     9961 gtcaaaacct gttaatagac gatccactac aatcgctaaa tcgatttgtt tcccaaattg
    10021 tttgaattcc gctctttttc gtgccaaacg attattgatg tctccgttat aacgctcaat
    10081 gtctgctaat gaccaggaag tatcataata ctgattatat tcctcaataa ttttttgcat
    10141 ttcttcttgt tgggcacttg aatctcggct attttcttct aatgaatagg taatagcgat
    10201 tcgtggaaaa tcaggatcct ccatcgttcg tccttcacga atgagctgat tgggaaactc
    10261 ttttgttaac caatcaggct ctttcgtcat ctctttgatg gcccgataat actttttggc
    10321 catgtcaatg gaactcgtgg ttaagattgc agatttcgtt ggcctgccat ttcgaaaatc
    10381 aaattttgta taggcattat ctggacggaa cattttatga atgacttttt ggatatgttc
    10441 atctgcttca aaaacagctg ggtccaaata actttctttt ttgacaggtt ccatttgatc
    10501 aatcatacga ttgatctgtt ctgatgaata ctctgcatat gtttctactt cttttaattt
    10561 ccgatagatt ttattctcta gtgaagtcgg ctccacggtg ttttcatgct ctacttgaaa
    10621 tcctaagaca gagccatctt ctagcgcatt cttaatggta taggtatgca agacctctcc
    10681 atactgatca tgggttgtac gtgccaattg ccctttggct tgtttccgat tctcaggaaa
    10741 aatcggtgta cctgtaaagc caaaccaagt ggatttcgga aacatttttt tgatttcttc
    10801 catattttca gcacttaacg ctcgatgaca ctcatctact acaaacacga tatgttggcc
    10861 cattaatttt tggaagcgat tggttccttt tttctcttct tgtttctttg catatttcaa
    10921 cgccgcatct agtttttgac gggttgtaat gattacaaca tttgcattgg catctgccaa
    10981 cagggtttca cttaattctt tggcacttcc cgttccaacg atcaagctat tcgctttggc
    11041 gttcccggaa gaaatcccag tattaaattc cgatgcaaat tttgtaaact ctgtggtggt
    11101 ttgattatct aaatctttgc ggtccactaa catgatcgtt cgatcgatcc ccgattttcg
    11161 tgctaaaagc ttagtagata caaaactagt caacgtcttt cctgaaccag tcgcatgcca
    11221 cacataccct gattgatgtt tatttgcggc aat
//
LOCUS       DACCQP010000087        11098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCQP010000087 DACCQP010000000
VERSION     DACCQP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.090"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVO87_002390"
     CDS             complement(99..1229)
                     /locus_tag="IVO87_002390"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7450547.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1788..2861)
                     /locus_tag="IVO87_002391"
     CDS             complement(1788..2861)
                     /locus_tag="IVO87_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7450548.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRK"
     gene            complement(2858..3685)
                     /locus_tag="IVO87_002392"
     CDS             complement(2858..3685)
                     /locus_tag="IVO87_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450549.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3686..4492)
                     /locus_tag="IVO87_002393"
     CDS             complement(3686..4492)
                     /locus_tag="IVO87_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450550.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4504..5589)
                     /locus_tag="IVO87_002394"
     CDS             complement(4504..5589)
                     /locus_tag="IVO87_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450551.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5606..6148)
                     /locus_tag="IVO87_002395"
     CDS             complement(5606..6148)
                     /locus_tag="IVO87_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450552.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            6406..7395
                     /locus_tag="IVO87_002396"
     CDS             6406..7395
                     /locus_tag="IVO87_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7450553.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7392..8129
                     /locus_tag="IVO87_002397"
     CDS             7392..8129
                     /locus_tag="IVO87_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7450554.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            8126..8677
                     /gene="coaC"
                     /locus_tag="IVO87_002398"
     CDS             8126..8677
                     /gene="coaC"
                     /locus_tag="IVO87_002398"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7450555.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            8693..9325
                     /locus_tag="IVO87_002399"
     CDS             8693..9325
                     /locus_tag="IVO87_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7450556.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            9410..10057
                     /locus_tag="IVO87_002400"
     CDS             9410..10057
                     /locus_tag="IVO87_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7450557.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            10062..11045
                     /locus_tag="IVO87_002401"
     CDS             10062..11045
                     /locus_tag="IVO87_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7450558.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         3410 a         2263 c         1999 g         3426 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaatt ctaagaatag gtcattatag atgcctaggt atttcgatcc tagatctttg
     1861 tagacttcaa gatgggagat cgtgtcatca gatggataga attgttcgtc cgacgaaatg
     1921 tcttttggca ataacgcttt tgcttcttca tttggtgtag aataaccaat atactctgca
     1981 ttttgtgccg cattttccgg ttctagcata aagttgatga aatcataggc accttcttta
     2041 tttttcacgg tttttggcat aacgatatta tcaaaccaaa gattggatcc ttctggtggg
     2101 atcacataat gtaaatgttc attttcccac atcatatcgg cagcttcgcc agaaaatgtc
     2161 actgccacgg aggcttcttc attgatcata tacattttga tctcatctgc cacgattgct
     2221 ttgacatttg gtgttaaatg attcaattta tttgctgctt ctgtcagctg tgccatattc
     2281 ttactattca aagaatagcc taaactattc aaagaaagcc ccataacttc tcgtgcaccg
     2341 tcaatcagca tcaaactgtc ttttaattcc ggcttccaca aatcatccca atgcttaatt
     2401 tgatttgcat caaagaattt atcgttatat acgatcccta aagttcccca aaaatatgga
     2461 atcgaatact gattatctgg atcaaaatcc aagtctaaaa atcgtggatc aatattattt
     2521 aatcctttga tcttcgaatg atctaatgga agtactagtt tttctttgat cattttttgg
     2581 atcatatatt cacttggtat agcgatgtcg tagtttgtcc caccttgctg gatttttgtg
     2641 aacatcgctt cattagaatc aaatgtttca taattcactt tgtatccata ttctttttca
     2701 aatttcgtga tcaaactagg atcgatataa tctccccagt tatagatggt aagtaccttt
     2761 gctccactca tgccacttgt tttttcaagt tgacgaacac caagcagcag caccgcaata
     2821 atgatgagaa tacctaaaaa cagagattgt aattttttca tcgcagccgt gccaccccct
     2881 cgcgtactct tctgtttttc ttcagccgtt ttggacggtt ttctttacta ataaagtagt
     2941 atccaatgac caatatcatg gagaacaaga aaactaacgc acttagcgca ttgatttcta
     3001 aactgattcc ttgtcttgca cgagaataga tctctactga gagcgtcgta aagccatttc
     3061 ctgtgacgaa aaatgtaacc gcaaaatcat caagagaata ggtaaatgcc ataaaatatc
     3121 cagctatgat tcctggagaa agataaggca aaatgatatt tttgactact tggaaattat
     3181 tagcaccaag gtctctagcc gcatcgacca ttgaatcgtt catttcctgc aattttggca
     3241 agaccatcaa gacgacgatt ggaatgctga aagcaatatg ggaaagcaat acactctgaa
     3301 aacctaatcc gaatcctagt agtgtaaaga aaatcaagaa actagcaccg ataataacat
     3361 caggagagac taataagatg ttattcatgc tcaacaacgc tgtccgtgca cgtcgttttt
     3421 tcgtgtaata gattcccata gccccaaatg tcccgatgat cgtggcgatc aatgcagata
     3481 agaaagccag catgaacgta ttcaatacga taatgatcaa acgtgtatct tcaaaaacag
     3541 cttcataatt ttcccaagta aatcctgtaa agctgttcat cgttcctcct tcattgaagg
     3601 aataaaaaat cagatagaag atcggtgcat ataagaataa aaagacgatc cctaaataaa
     3661 gatacgacca tttaaatttt ttcatttatg agcgacctcc ttttttcttc tcgcctgtca
     3721 ggagcatcac gatgaacata gcaatgatta aaatcacacc gattgtagaa cccatccccc
     3781 agttttgtgt aaccaagaaa tgctgttcga tagccgttcc taaagtgatc acccgatttc
     3841 cgccaatcag acgagtcagc atgaataaag aaagagaagg aataaatact gcttggacac
     3901 cacttttcac accatttaat gataacggaa aaatcactcg tcggaaggtc tcaaagctat
     3961 tcgcgcctaa atctctacta gcactgatca aagaaggatt catctcttcc aatgcattga
     4021 aaattggcat aatcataaat ggaatctcga tatacgttgc cacgaacaga aaactgaagt
     4081 ctgtgaataa tatctgtttc ggcccgatcc cgataaaatc aaagaaatga ttcacgctgc
     4141 cgtgcacact gaaaatccca ataaatgcgt aagctttaag cagcagattc acccatgtag
     4201 gcaaaataat cagcatcagc cataattgct tgtgcttcag ttttgttaaa aaataagcag
     4261 tcgggtaact gatgatcagc gtgaaaaacg tgatcaaaaa tgcataccat acagaattga
     4321 tcgtcataga aagcgatgtc cccgacgtaa aataagatag ataattattc agcgtgaatt
     4381 gcccattcat atcaaaaaaa gattgataaa tgatcattaa tacaggcgcg atcacgaata
     4441 aaccgagcca caaaatataa gggacggaat agatcctgcg cattgttttc attcttgtcc
     4501 cccctattct tcgtagctgt caagacgtgc atcgaattct tcttcagact cgttgaaacg
     4561 catgacatgg atatcttctg gctcaaaggc caatcctact ttgcttcctt ctttagcttt
     4621 tttcgttgaa tggaccatcc attcattttg ctgttcatca taacagatga tttcataatg
     4681 gacaccacga aacagctgag tatcgaccgt tacgactagt tttcctttat ctggtgtcgt
     4741 gatcgtcaaa tcctctgggc gtaaaacaac ttcaactggt tcatttagac gcatcccgcc
     4801 atcgacacat tcaaattgct taccaacaaa ctcaactaga ttatcttcga tcattacacc
     4861 gtccacgata ttgctctccc caacaaaatc agcaacgaaa tgattgattg gctcatcata
     4921 aatatctacg ggtgtaccac tttggacgat tttcccttta ttcatgacaa aaatctcatc
     4981 actcattgcc aacgcttctt cttggtcatg agtaacaaaa ataaacgtga ttcctaatcg
     5041 ttgttgcaga tcccgcagtt catactgcat atctgtacgt agtttcaaat caagcgccga
     5101 taacggttca tctaatagca atactttcgg ttcatttaca attgctcgtg caatcgctac
     5161 acgctgtctt tgaccaccag acatttcact aatctcccgt ttttcgtatc caggaagctg
     5221 gaccatacgc aaagcatctt ttaccttctt ttcgatatct gatttggaca ttttcttgat
     5281 cttcaatcca aaagcaacgt tatcaaaaac attcatatga ggaaaaagtg cataatcttg
     5341 gaagactgta tttacttgtc gcttattagc tggcaaatca ttcaaccgtt tcccttcaaa
     5401 atagatgtcc ccttctgtag cttcggtgaa tccagctata atacgtagga tcgttgtttt
     5461 accacagccg gaaggaccaa gcagtgtata aaattttcct tgttcgatct caaaacttac
     5521 tttttttaat actgtttcat catcatagcg tttcactact tgatcaaatg aaatgattgg
     5581 tttccccacg tttttacccc ctatattaca aatatgattg cgttgcaaca atcaatactt
     5641 ttgttcgttt tttgagatga ttccttaatt gatgttgttt tgtcgcttga taatagatcg
     5701 actgtcctgc ttttgccaca tagaccgtct ctcctaagct taactctaat tccccttcta
     5761 atacaaaaat aaacgtttca gataatgaag gttcaaacgt cttatattct cctgcacctt
     5821 caaaagtcag caagacaggc tccatttctt tttcattcga atcagccacg agccattcca
     5881 attcataccc gttttcttca tcgaaataaa ccgtatgatc ttcttttgaa taaaccactt
     5941 gcagattcgc tgcttcttga tgaaaaaatt cttctggagt cacacctaag acttcaagaa
     6001 tagaaaagaa tgtttccatt gatggtgaac ttaaatctcg ttccaactgg gaaatatacc
     6061 cttttgacaa atctgtgcgt tcgcctagtt cttcttgtgt caaattcttt tggatgcgca
     6121 aatttcttaa cttctcacca atttccataa tttcctccga atcgaagttt tctaatagta
     6181 aacttcctgt ttactaccac tcgtattagt atacgaaatt tattgaaaaa ggcaaccctt
     6241 tgtaaaattt tttttgctga atatttcttt atcttttgga ataaattcta gttgtttgat
     6301 tctttatttt tcacctaatt tttgtctaaa tttacggaag aacaagaata aaagagtagg
     6361 ctttatggta tgataaaaga aaattttaaa aggagtgtct cacgaatgaa cgcttttcga
     6421 tcttttcagg taataaagga acctgttttc catacagcaa tcagtgatct gccatttact
     6481 gagttggctg aaaatgaagt cttggtaaaa attgcctatt ctgatgtcaa ttataaagat
     6541 gcattggcaa tgagcgagtc tggtcaggtg atacgaacat atccgatgac tccagggatt
     6601 gatttatccg gaactgtcgt ccaaagtaac aatccacgtt tttcaaaaga agatcttgta
     6661 cttgctactg gttttgggct tggcgtcacc catccaggcg gttatagcca atatcaaaaa
     6721 gtaccaggag actggttagt tcctctacca aaaaatatga cgctgcgtca agcgatgatc
     6781 cttggtacag cagggtttac tgctatgttg tgtgtgaacg ctttaatccg tcaaggtatg
     6841 acctccgata aaaaagttgt cgttactggt gcttctggcg gtgtaggcag tacagcgata
     6901 gccctgttac ataaactagg attccagtca atcattgcct tttccagaaa agaagagtct
     6961 gtcacatggc taaaaagtct aggtgctttg caagtcgtac gacctgatga atttctgcca
     7021 gagacaacaa aagctttagg aaaacagcaa atcgactatg tcatcgacac agtaggtggc
     7081 gagcagctaa gcagtcttct tccattgatc tcttataacg gtgcagtagc tttatgtggc
     7141 aatgctgggg gaatcaaact aaacgcgact gtcttgcctt ttattctgcg aaacatccag
     7201 ttgattggaa ttgattctgt caatgtacca atagatcaac gtctttcttt atggcaacag
     7261 atggcggatt tgcagattgc agatgaactg gtcgtccaag agattactct ggatcaattg
     7321 cctgaaacag catctaaact acttgctgga acacaccaag gaagaacact agtaaatgta
     7381 ggtgaccata aatgaagatt ctaatcacag ctggaggaac gactgaaaaa atcgatcaag
     7441 tacgtgctat tacaaatcat tcgacaggac gattagggca agcattagct gattatttag
     7501 ctgccaatcc ggataccaca atcgattacg tgactacgaa acaagcactg aaaccgaaaa
     7561 gacactccaa tataacgatt tatacgatcg aatctgcact ggacttattt ttacaattag
     7621 aagaactaac gaaaaaagaa cactacgatg cgattatcca cagtatggca gtcagtgatt
     7681 ttactcctgc ttttagtttt tcagaagaac agctggcaaa aaacctaccc acttcttcaa
     7741 cgcaagaaga actggacaat tggtttgcgg aaaatgaaca gacagatacc actgtttcaa
     7801 agatctcttc taacaccgag catcttgtgc tcgttttgaa gaaaacacca aaaatcattt
     7861 cttatctacg tgaatggcag ccaaaagcaa aaatcattgg tttcaaacta ttagtagatg
     7921 taccaaagga atctcttctg gcagtcgcaa aaaatagtct gataaataac aaaactgatt
     7981 ttattttcgc aaatgatttg acggaaatcc atggcgagac acaccacggc tatctcttat
     8041 ctaaagatgg aacagtggaa gaagctcaaa gtaaatctga gattgctgca ttgattactg
     8101 agaaaattag actggaggaa tcaaaatgaa aaagatctta ctaggcgtta ctggaagtat
     8161 ttctgcttac aaaagcgctg atcttacaag tcaattcgtc aaattaggtg cccaggtaga
     8221 tgtgattatg tctaaaagca gcacacattt tatcacgcca ttaaccttgc aatctctgtc
     8281 gaagcgcgca gtccatacag acgtcatgca agaatcaaat ccgcaggtaa tcaatcatat
     8341 cgaactagca aaacaagctg acttgtttct catcgcgcct gctacagcaa acattatcgg
     8401 caagctagca aacggcattg cagacgatct tctttcaaca gtcgcaatgg cgcttccagt
     8461 gggaacacca aaattgattg cccctgccat gaacacgaat atgtatcaaa acccgatcac
     8521 tcagcgtaat ctggaaacat tgagatctgt cggctatcag gaaattgaac caagagaatc
     8581 tcttttagct tgtggagatt ttggaaaagg tgccttagct gatattcaga cgatcatcga
     8641 acaggtcatg ctgactctta atgaaagaaa tgcttagaaa aaaggagtac ttatgaaaaa
     8701 tacaaaaaac tttacattaa ctgcgatgtt tttagctatt ttgattttat tagctgttac
     8761 gcctttaggt tttatcccta tcggtccaat caatgcaaca accatgcata ttcctgtaat
     8821 cattgcttcg atcgttttag gaccaagact aggtgctttt cttggcggta catttggact
     8881 gatcagcatg atccgtagca cagttatcca aacacctttg tcttttgtct tttctccgtt
     8941 tatcccagtc atcggaacag aacatggaag ctggaaagcc ttgctgatcg cgtttattcc
     9001 tcgcatcttg attggggtgg ttccttattt cgtttataaa ggtctgcgca aactaatgaa
     9061 acaaaaagca gattccgttt ctttattctt agctggactt tccggttcat tgaccaatac
     9121 gattttagtg atgaatatga tctacttttt attccagcaa gattatgcca aagtcatcgg
     9181 aacaaattta aatgctgttt attcagctat tctagcggtt atttttacaa gtggtatacc
     9241 tgaaggaatc gtcgcaggtc ttgcgactgc agcagtatgc aatgtcttgt taagatactt
     9301 caagcaccct tctcttacta actgatagtc atcgtagccg atctctgatt tttttctctt
     9361 ttggtgttac tattgaatgg aaagatttga cgtcgaaagg atgatccaaa tgaaaatttt
     9421 tgttgtaggt gcaaacggac aaattggccg acacctgata aaagatttag ctccaagttc
     9481 ccatgaaatt tttgcagggg tgagagatgt tgcttcccaa acattagtaa aaaaagagaa
     9541 tgtttcgtat gtctcttttg atttaacttg gtccgtggaa aaaatgaccg aagcattcaa
     9601 aggcatcgat gtattgattt ttgctgccgg ctctcaagga aagaatcttc tgcaagtaga
     9661 cttggatggc gcaatcaaaa cagtgatcgc agctgaaaat gcgcatgtct ctcgctatct
     9721 gatggtcagt gctgtttatg cagatgaacc agcaaaatgg cctgagtcca tgacagatta
     9781 ttatattact aaacattatg cagatgaatg gctgaaacga actaacttag attttgtcat
     9841 tcttcaacca gtcacattga ctaacgatga agaagttact tccattcaat taactaagcc
     9901 taatgagaag gcatcaaaaa cgatcactcg ctctacagtt gctgctgtac ttgccgctct
     9961 agtggaagaa acggatattt ctcgtacgac tctagtactt tctgaaggta gcaaagagtt
    10021 gaatacagca ttccaagaat gggcaaagga ggaatagatc catgcgtgcc atttcaatca
    10081 agcatcctgg ttctgtagat gtattagaac aaattgaagt gcctgttcct aaaccaaaac
    10141 aaggcgaatt actgatcaaa gtccatacag cagctgtcaa tcgaacagat attatgaaaa
    10201 gagaatcagt ctctttgaaa cctccctacc ctattttggg agtcgaagta gctggagagg
    10261 tagttgagaa taaaagtgat atctccactt ttactcctgg gacaaaagta tacggattag
    10321 tcaatctagg tggttatgcc gaatatgctg tgatgcctgc cgatcgtgca atccttctcc
    10381 ctgatacgct agactatgtt tctgctgctg gaatctcaga agtttttttg actgcttatc
    10441 aaacacttta ctggcttgga aaattacaag aaggcgaaac tgtcttgatc catgcaggag
    10501 caagtggtgt gggaacagca gcgatccaat tagctagaca gctttctcac gcgaaaatca
    10561 tcgttactgc aggatcagaa gaaaaactcg ccttttgtaa agaacttggt gcagatgaat
    10621 tgatcaacta taaaaaacaa gactttcaag aagaggtacg aaaaatcact gaaggtcggg
    10681 gagtcgatgt tattttggat ttcatcggcg cttcttactg ggaaaagaat ttagcttcta
    10741 tagccgttga tggacgttgg gtcttgattg gtatgctagg tgggaccatc gtgcctgaga
    10801 tagaccttgg tgtattgatt gggaaacgtg tccaactgct tggtacatta ttgacaccta
    10861 gaagtgatca gtataaagca gagctcgcac aaacttttat gcaaaaagct gggccttttc
    10921 tagaaaatgg caagattcgg ccaatcattg accgtacatt ccaattcagc gcagtcaaac
    10981 aagcgcatga atatatggaa gcaaacaaaa acatcggaaa aatcattttg aagataactg
    11041 aataaaatca tgctgatcaa aaaagaggct gggacagaag cgtttaactc caagaaat
//
LOCUS       DACCQP010000088        10715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCQP010000088 DACCQP010000000
VERSION     DACCQP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.091"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(258..1106)
                     /locus_tag="IVO87_002402"
     CDS             complement(258..1106)
                     /locus_tag="IVO87_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7450559.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1270..2172
                     /gene="gnd"
                     /locus_tag="IVO87_002403"
     CDS             1270..2172
                     /gene="gnd"
                     /locus_tag="IVO87_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7450560.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2174..3175
                     /locus_tag="IVO87_002404"
     CDS             2174..3175
                     /locus_tag="IVO87_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7450561.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3172..3456
                     /locus_tag="IVO87_002405"
     CDS             3172..3456
                     /locus_tag="IVO87_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7450562.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3489..4286
                     /locus_tag="IVO87_002406"
     CDS             3489..4286
                     /locus_tag="IVO87_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7450563.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4300..5133
                     /locus_tag="IVO87_002407"
     CDS             4300..5133
                     /locus_tag="IVO87_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7450564.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5151..6008
                     /locus_tag="IVO87_002408"
     CDS             5151..6008
                     /locus_tag="IVO87_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7450565.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6030..6539
                     /locus_tag="IVO87_002409"
     CDS             6030..6539
                     /locus_tag="IVO87_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7450566.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6576..6977
                     /locus_tag="IVO87_002410"
     CDS             6576..6977
                     /locus_tag="IVO87_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450567.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7089..7442
                     /locus_tag="IVO87_002411"
     CDS             7089..7442
                     /locus_tag="IVO87_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450568.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7476..7919
                     /locus_tag="IVO87_002412"
     CDS             7476..7919
                     /locus_tag="IVO87_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7450569.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7936..9021
                     /gene="uxuA"
                     /locus_tag="IVO87_002413"
     CDS             7936..9021
                     /gene="uxuA"
                     /locus_tag="IVO87_002413"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7450570.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9014..9727
                     /locus_tag="IVO87_002414"
     CDS             9014..9727
                     /locus_tag="IVO87_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7450571.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10479..10679
                     /locus_tag="IVO87_002415"
     CDS             10479..10679
                     /locus_tag="IVO87_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7450572.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3591 a         1692 c         2316 g         3116 t
ORIGIN      
        1 tttagaaaat aagaaacaaa aataataaaa aaaagaaaag ataaattttt tactactgta
       61 tactaacttt gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca
      121 aagatgcgat gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag
      181 aaccaaaaaa gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg
      241 ctttttctgt tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata
      301 gaacgacgtg attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg
      361 cttagttgag aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata
      421 gaaataaaaa cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg
      481 attgtttttg ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc
      541 cctgtgtgag aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat
      601 tgaatatggt aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa
      661 gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca
      721 ttgataatag tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt
      781 aaagttgaaa tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc
      841 tgaatatttt cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt
      901 ttaaaatctt taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca
      961 gcaatgccca gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt
     1021 tctaaaatat aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg
     1081 atttgtagtc ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt
     1141 ttttccgttc tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat
     1201 atttttgtaa aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga
     1261 ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta
     1321 aacttaaaag agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt
     1381 caggcaagag aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt
     1441 gagcagaaaa aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta
     1501 agaaaacttt ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat
     1561 tataaagatt caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat
     1621 tgtgggactt ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga
     1681 gataaagaaa cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga
     1741 tatctgtatg ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc
     1801 gaatatggaa tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat
     1861 gcttttgact tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg
     1921 ctgatggaaa tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga
     1981 atcgtttctg catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt
     2041 ccagttccag taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt
     2101 ttttcagcaa aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa
     2161 aagggtgagt aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag
     2221 agtctttcca caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt
     2281 tgatcaagag attccaggaa aagaactagc ggagttactt aaaggattca atatcattat
     2341 tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt
     2401 gatttcccga catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca
     2461 gacaatcgta tctatcatac ctgctttagt tgaacgaaat gctgtagcag aaaataatat
     2521 aactaatttg ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga
     2581 tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt
     2641 gattggtgca ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg
     2701 tgatgttcta gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa
     2761 aaaagtagaa cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt
     2821 gacagaagag aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt
     2881 ttatctatct aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct
     2941 gaaaagcgga aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca
     3001 agatcatccg tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac
     3061 aagggaatgt ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca
     3121 gcaccttctt cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag
     3181 aaaatggtga acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt
     3241 ggtatttcaa aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc
     3301 cacggaacgg tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg
     3361 attcaatttt tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata
     3421 gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga
     3481 ccgatctagt gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt
     3541 tagctagtaa ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag
     3601 cagaagggaa aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat
     3661 tagtgattga ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat
     3721 ggtatgaatc tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc
     3781 tagcacaaga tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa
     3841 tccaagatat ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct
     3901 ttgctgttgc tggtttagtt ggaggattag gagataaata cgcaagaaat gtggcagcac
     3961 atgctatgca tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag
     4021 aaaaagtagc gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag
     4081 acgagttata tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat
     4141 tatttccgaa agataaacag ataatagata aaatggctga atttattgat tcgaaagaga
     4201 aagtgcattt gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac
     4261 tggaaggtta tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta
     4321 caaagtttgt tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga
     4381 gccacgttga ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg
     4441 ggggaagttg cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt
     4501 ttttctccag gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca
     4561 gtcgcattat taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga
     4621 ttgctcggaa gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat
     4681 acagacaaag ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat
     4741 ccaacggttg cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg
     4801 gcgccattga tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt
     4861 gaagtagtag gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt
     4921 cgaaaagatt atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct
     4981 ttagatatta cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc
     5041 cagtcgcaaa aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt
     5101 gatgacgaag atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa
     5161 taatggataa tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct
     5221 atctgagatg gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat
     5281 cactactata cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa
     5341 aagcagcata taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg
     5401 tgattactgg cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa
     5461 aagagatctt gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac
     5521 tagcaggaat cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg
     5581 cggtaccttg ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg
     5641 ggttgtatca ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta
     5701 atgctacagg cctaaagcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt
     5761 tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga
     5821 aatttgcgat atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag
     5881 ggatattacc tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga
     5941 aagtcacaca agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta
     6001 ttttataaaa agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg
     6061 agcgtttgat ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca
     6121 tttttgttgt agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg
     6181 caggaagccg tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt
     6241 gggatgaata tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga
     6301 tttacgactt agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc
     6361 aaaaaaatcc tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg
     6421 ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca
     6481 ctccatctgc acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa
     6541 caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg
     6601 attgggaatt tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc
     6661 gttttctttc agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg
     6721 cttacgatac gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag
     6781 agaaagttat ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt
     6841 ttgattggaa agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt
     6901 tgttcagttt aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg
     6961 atctgctatc cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat
     7021 tgggaaaagc agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca
     7081 tattaacgat gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg
     7141 ttgatgaagg ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag
     7201 aggctattct tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa
     7261 attatttttc aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg
     7321 tcaattatat ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag
     7381 atttacgagg gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat
     7441 aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac
     7501 atggtaagtt gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc
     7561 atattgaaac aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca
     7621 aagaaaaatt aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta
     7681 gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag
     7741 cagccactta tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc
     7801 agatgcttca cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata
     7861 atttaactgt ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga
     7921 aggaaggctg aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc
     7981 ccattaaaat atgttcgcca aataccggga ataaatggga taattggaac tttgctgact
     8041 aaaatgcctg gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag
     8101 agccaaggac tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca
     8161 gggacagatg aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct
     8221 cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact
     8281 gatttgttta atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc
     8341 gatcagatgg agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa
     8401 ttgccgggtt gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa
     8461 ggaatgaccg aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca
     8521 gtgtgcgaaa aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt
     8581 tttggttttc ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta
     8641 gattctccat ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac
     8701 gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac
     8761 gtggagtttt taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc
     8821 cttgatatgt atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc
     8881 cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat
     8941 cgtgcaatgg gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca
     9001 ctgggagagc attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt
     9061 agaaactgga cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga
     9121 aatgcgtaag gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca
     9181 aatgggcatt ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca
     9241 atataaagaa aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga
     9301 aattttatct tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg
     9361 ggtaaaagta gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat
     9421 ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc
     9481 aaaaatgaat attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga
     9541 agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc
     9601 cgatcataat gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc
     9661 agactatgaa ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta
     9721 tctctaataa agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg
     9781 aacagatgaa aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa
     9841 tttagaacaa acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag
     9901 aacgagaatc gtccatatga gggtttccac tccttatgca gattttttta attcttgcac
     9961 tgcttgttcg atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac
    10021 aaatagattc aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt
    10081 catagccatt ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa
    10141 attccattta taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc
    10201 aaaagttgca gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt
    10261 caactttttc ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta
    10321 tccttgtgtt attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat
    10381 ttgagcgata tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga
    10441 aaaaacgtac attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat
    10501 ggttcaacgc agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg
    10561 cacatttttc agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt
    10621 cttacgatgt tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat
    10681 aaaaattgac ttttaaccgt cttttcgaaa gaaaa
//
LOCUS       DACCQP010000089        10500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCQP010000089 DACCQP010000000
VERSION     DACCQP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.092"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(210..962)
                     /gene="xth"
                     /locus_tag="IVO87_002416"
     CDS             complement(210..962)
                     /gene="xth"
                     /locus_tag="IVO87_002416"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7450573.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1075..1995
                     /locus_tag="IVO87_002417"
     CDS             1075..1995
                     /locus_tag="IVO87_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7450574.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..3003
                     /gene="murB"
                     /locus_tag="IVO87_002418"
     CDS             2068..3003
                     /gene="murB"
                     /locus_tag="IVO87_002418"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7450575.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3301..4779
                     /locus_tag="IVO87_002419"
     CDS             3301..4779
                     /locus_tag="IVO87_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7450576.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="IVO87_002420"
     CDS             5056..7116
                     /locus_tag="IVO87_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450577.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEVHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="IVO87_002421"
     CDS             7199..7768
                     /locus_tag="IVO87_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450578.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="IVO87_002422"
     CDS             complement(7943..8749)
                     /locus_tag="IVO87_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450579.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="IVO87_002423"
     CDS             8887..10401
                     /locus_tag="IVO87_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7450580.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3724 a         1749 c         2068 g         2959 t
ORIGIN      
        1 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
       61 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
      121 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
      181 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
      241 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
      301 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
      361 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
      421 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
      481 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
      541 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
      601 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
      661 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
      721 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
      781 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
      841 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
      901 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
      961 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
     1021 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
     1081 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
     1141 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
     1201 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
     1261 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
     1321 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
     1381 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
     1441 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
     1501 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
     1561 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
     1621 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
     1681 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
     1741 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agatgctgat
     1801 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
     1861 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
     1921 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
     1981 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
     2041 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
     2101 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
     2161 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
     2221 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
     2281 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
     2341 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
     2401 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
     2461 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
     2521 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
     2581 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
     2641 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
     2701 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
     2761 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
     2821 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
     2881 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
     2941 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
     3001 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
     3061 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
     3121 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
     3181 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
     3241 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
     3301 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
     3361 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
     3421 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
     3481 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
     3541 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
     3601 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
     3661 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
     3721 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
     3781 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
     3841 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
     3901 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
     3961 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
     4021 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
     4081 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     4141 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     4201 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     4261 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     4321 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     4381 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     4441 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     4501 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     4561 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     4621 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     4681 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     4741 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     4801 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagttcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
//
LOCUS       DACCQP010000090        10488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCQP010000090 DACCQP010000000
VERSION     DACCQP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.093"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IVO87_002424"
     CDS             complement(<1..357)
                     /locus_tag="IVO87_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7450581.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IVO87_002425"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IVO87_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7450582.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IVO87_002426"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IVO87_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7450583.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IVO87_002427"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IVO87_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7450584.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2237..2869
                     /locus_tag="IVO87_002428"
     CDS             2237..2869
                     /locus_tag="IVO87_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7450585.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="IVO87_002429"
     CDS             complement(2871..3776)
                     /locus_tag="IVO87_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7450586.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4514)
                     /locus_tag="IVO87_002430"
     CDS             complement(3861..4514)
                     /locus_tag="IVO87_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7450587.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="IVO87_002431"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="IVO87_002431"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7450588.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="IVO87_002432"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="IVO87_002432"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7450589.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="IVO87_002433"
     CDS             6763..7848
                     /locus_tag="IVO87_002433"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7450590.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="IVO87_002434"
     CDS             7845..8909
                     /locus_tag="IVO87_002434"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7450591.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="IVO87_002435"
     CDS             complement(9158..9961)
                     /locus_tag="IVO87_002435"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7450592.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IVO87_002436"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IVO87_002436"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10488)
                     /locus_tag="IVO87_002437"
     CDS             complement(10268..>10488)
                     /locus_tag="IVO87_002437"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7450593.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3100 a         2219 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgccc
//
LOCUS       DACCQP010000091        10145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCQP010000091 DACCQP010000000
VERSION     DACCQP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.094"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            131..451
                     /locus_tag="IVO87_002438"
     CDS             131..451
                     /locus_tag="IVO87_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450594.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            903..1103
                     /locus_tag="IVO87_002439"
     CDS             903..1103
                     /locus_tag="IVO87_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7450595.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1250..4039
                     /locus_tag="IVO87_002440"
     CDS             1250..4039
                     /locus_tag="IVO87_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7450596.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4086..4613
                     /locus_tag="IVO87_002441"
     CDS             4086..4613
                     /locus_tag="IVO87_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7450597.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4634..5824
                     /locus_tag="IVO87_002442"
     CDS             4634..5824
                     /locus_tag="IVO87_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7450598.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5864..7162
                     /gene="asnS"
                     /locus_tag="IVO87_002443"
     CDS             5864..7162
                     /gene="asnS"
                     /locus_tag="IVO87_002443"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7450599.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8338..9012
                     /locus_tag="IVO87_002444"
     CDS             8338..9012
                     /locus_tag="IVO87_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7450600.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9009..9350
                     /locus_tag="IVO87_002445"
     CDS             9009..9350
                     /locus_tag="IVO87_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450601.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9380..9739
                     /locus_tag="IVO87_002446"
     CDS             9380..9739
                     /locus_tag="IVO87_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7450602.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3388 a         1812 c         2170 g         2775 t
ORIGIN      
        1 ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc atttatgaac
       61 aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac ttatcgataa
      121 ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc taagtttaac
      181 aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca cacgtctgta
      241 caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa cagaagaacg
      301 gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt atgaaagttg
      361 ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc agaaatatga
      421 tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa actaatctcg
      481 ttggtcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca gaaataccat
      541 gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac agaaccgata
      601 agtagtcaga aagaataatt tttttagttt aaaaaagaac atcagagcaa aaaaacacca
      661 actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat gtaatcgctg
      721 agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa acggtatgat
      781 atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa taaacatctt
      841 ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg aggattttta
      901 ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga ttcatcactg
      961 cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac ggattcaaaa
     1021 ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc ggacctcaag
     1081 caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc agacatttgc
     1141 gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt tctgcacttt
     1201 gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt tgaaaccaag
     1261 aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag atcggatcat
     1321 ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt cgatcgatat
     1381 tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca cgaatcaacg
     1441 cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc ttttggcaga
     1501 caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc atgaactcat
     1561 gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac ttgcacaaat
     1621 tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc ttggatttat
     1681 tcatgagaat cctcatcagg cagatagtga tgccgaagtg acaggtcaac ttcttttgct
     1741 gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg cgcgattaag
     1801 tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag aaatgaaaga
     1861 aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt tgcagaaaaa
     1921 agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa gaaaaaaaca
     1981 agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga acagactgat
     2041 gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga tcgaagcagc
     2101 tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg ctacacctga
     2161 aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca tagaaaaaga
     2221 cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta tcaaaagcta
     2281 tcgccactat attgatttgc agcgttttaa aggcacactt gtggaaccgc cagaacaaaa
     2341 acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga agactggtga
     2401 tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata tcgcgcacag
     2461 aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg ttcgtttctt
     2521 gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt tagtacaaga
     2581 aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg atgaagtaca
     2641 tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta actatttcca
     2701 taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac aaagctattt
     2761 accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac tttctgcgct
     2821 atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg gcaagcaacc
     2881 taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc agctgccact
     2941 ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc agcagctaca
     3001 gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc agatgcggat
     3061 cgatttagca agtttatata gcctatttga tcaattagac aagcaatact tgctgatgga
     3121 tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg ggaacacagc
     3181 gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt cgactgtttg
     3241 gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg gccctaatcg
     3301 acactatctc tctaagaggc ttggcattga agcagcacct cttaaagtga ttgcctctcc
     3361 ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg ccgtccatca
     3421 agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc tgatcgaaca
     3481 agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga aggtctatca
     3541 gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag ggtttggtgg
     3601 aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat tgtttggcgc
     3661 tgacagcttt tgggaaggtg tagatttgcc tggagatgct ttgcagatcg ttgtagtcac
     3721 acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg cttatttaga
     3781 atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt tgaagttacg
     3841 ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc ttattttgga
     3901 tcgaagacta gtcactgcaa aatatggcgc acggatcatc catgcattac cgaaaaaagt
     3961 accgatcaaa gagctagcta tggaggaaat ctatcaggat atccatgaat tcttaaagaa
     4021 aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg ctataatgaa
     4081 aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag gaaaaaacga
     4141 ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg atcagctcga
     4201 ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca gtagaaatcg
     4261 caaagaaata tgcaaatctt gaggaaaccg atcattttta ttggtttact cgaaagaaga
     4321 catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt atcattccta
     4381 aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa aacgaagcaa
     4441 aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct ttagggatgt
     4501 atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa ctgaattatt
     4561 acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt taaaaggtaa
     4621 aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt ctgtcacttt
     4681 ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc tgagtttgac
     4741 tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa tcgaagcaat
     4801 ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc ttcgacaggc
     4861 aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac aaacagtcgt
     4921 aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg acccgcaaga
     4981 tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa aactggcaga
     5041 aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta ctgttgaaca
     5101 gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc catcgaatcc
     5161 gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg cagtaaaaaa
     5221 tgatattttg atagttgctg atgatattta tggccgttta gtttataatg gcaatgagtt
     5281 tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca tcaacggcgt
     5341 gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa atcctgtgat
     5401 catcaatgga atgatcgctg ttgcttccca atcaactagc aatccgacag cagtcagtca
     5461 atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa tgcggaacgc
     5521 ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg gtgtttctct
     5581 tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc tacgcatgtg
     5641 taagtatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag gggtagcgct
     5701 tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg ccactgatcg
     5761 cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta aaagtcaaaa
     5821 ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac aaattcaaat
     5881 catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg tcgcaaataa
     5941 gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct ttttccaagg
     6001 tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa atctacacca
     6061 agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa aatttggtta
     6121 tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc cgatcacacc
     6181 aaaagaacac ggaacagatt tcttgatgga tcatcgtcat ttatggctgc gttcttcaag
     6241 acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg aattctttaa
     6301 tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttctgcac cagaaggcac
     6361 tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc aaagtggcca
     6421 attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg gtccgacttt
     6481 ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga tcgaaccaga
     6541 aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg tagcattctt
     6601 agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac gagacaaaga
     6661 agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg atgcaatcga
     6721 attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg gttcacctca
     6781 tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact atccaaaagc
     6841 catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa tttgtgcaga
     6901 tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg caacagacta
     6961 tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat attcatggta
     7021 tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag gccttgaacg
     7081 aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat tcccacgttt
     7141 gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca agtaattaga
     7201 acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt accgcagatg
     7261 taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt ttcgagtatt
     7321 aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct taataccgag
     7381 aaactggctt ttgaacaccg tttaaggaag aggttgtgaa atcagtgcct tgacctgagt
     7441 attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt agctaatatc
     7501 gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc gttcagctcc
     7561 gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat tgtgacttgt
     7621 gaccgaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg aactctcgca
     7681 gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa gaaagcaaaa
     7741 gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca aacatataca
     7801 aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga aataaagcag
     7861 catctagtac ctgaaaacaa actaatagta acattcgaag atggagtagg tccgtatttt
     7921 cagcatgcaa tgatccatat gtaagtttaa ttcatccttg aaatcatcgg accagataaa
     7981 gttatgacca atctatccca agcaatctag aagagatatt agtaaaagaa catttactgg
     8041 aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt ctgattgacg
     8101 gaagcttagg tgtgatcgat ttctctggac caaacaacga aggaatcaag gggaatccgg
     8161 cacgttaaga aaaaaagtat aaaaaactag acacttgatg agcgttcatt caattgattg
     8221 aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct tgatagggct
     8281 tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt ttgacaaatg
     8341 aaaaaacgta tattatttat cggtgacagc attacagacg caggaagaga ccgagcagaa
     8401 gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat ctcccaatat
     8461 ccaacgatga aatttgaatg catgaaccgt gggataggag gagacaaaat tgcagatctg
     8521 caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc gattttgatc
     8581 ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac tgagaaatct
     8641 gcgcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac agcggaaatc
     8701 tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg tcatggctgg
     8761 cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga atatggcgca
     8821 ttgctggttc ctttagatgg catattcaat gctttaggga ttgcttatgg tttttcgaaa
     8881 tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat tgcacaaagc
     8941 tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga gcgaaagaaa
     9001 agtgagaaat gatggaaata cggtgggcag taaaagaaga tttacctcaa atcaatcagc
     9061 tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac ctaaatgata
     9121 agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga tttgcgcaag
     9181 cggacgtgca acagtcagaa gaacgggtgg atcagcgacc atcaggaaaa ctaatccaac
     9241 acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca gaatacttga
     9301 tgctggaagc taaaaaaaga agaataaaat caatttttgc ccagttttaa tcaaaaacag
     9361 taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta tattcggcgg
     9421 actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca cgatcatagg
     9481 tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc cttttggcaa
     9541 agaagtaatt tatcaccagt ctggcatgtc attgatcgtt aatatcatct ggctgatcgt
     9601 ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat gtatcacgat
     9661 catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt tgatgccatt
     9721 cggtgcagaa attgtataaa aagagaataa taaaggttag aaaacgcata gagtcaggtg
     9781 ggatacatgt atttcacctg actctatgtg ttttcctgta taagttctcc catgtgactt
     9841 ttacgtcttt tatttatatg ggattactgt ttatggaaag ttgaaataat tttcattaaa
     9901 gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga aggacatttt
     9961 gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga caaattgcca
    10021 gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag tgcgggttgg
    10081 ataagagcag gggcaatgat cgaagagaaa aagaaagaat aagccaaaac atctgtatgg
    10141 aagaa
//
LOCUS       DACCQP010000092         9882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCQP010000092 DACCQP010000000
VERSION     DACCQP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.095"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            3..359
                     /locus_tag="IVO87_002447"
     CDS             3..359
                     /locus_tag="IVO87_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450603.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            375..725
                     /locus_tag="IVO87_002448"
     CDS             375..725
                     /locus_tag="IVO87_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7450604.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            754..1533
                     /locus_tag="IVO87_002449"
     CDS             754..1533
                     /locus_tag="IVO87_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7450605.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1544..2221
                     /locus_tag="IVO87_002450"
     CDS             1544..2221
                     /locus_tag="IVO87_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7450606.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AILPAVN"
     gene            2240..3346
                     /locus_tag="IVO87_002451"
     CDS             2240..3346
                     /locus_tag="IVO87_002451"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7450607.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKANSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFARIAKEQILPIKTIIGGVIYDN"
     gene            3336..4427
                     /locus_tag="IVO87_002452"
     CDS             3336..4427
                     /locus_tag="IVO87_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7450608.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4440..5180
                     /locus_tag="IVO87_002453"
     CDS             4440..5180
                     /locus_tag="IVO87_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7450609.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5468..7408
                     /locus_tag="IVO87_002454"
     CDS             5468..7408
                     /locus_tag="IVO87_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7450610.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IGVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7495..9417
                     /locus_tag="IVO87_002455"
     CDS             7495..9417
                     /locus_tag="IVO87_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7450611.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9697..>9882)
                     /locus_tag="IVO87_002456"
     CDS             complement(9697..>9882)
                     /locus_tag="IVO87_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7450612.1"
                     /translation="LCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTEFRKVR
                     EGIKRKVLDLNQRTSIV"
BASE COUNT         3223 a         1636 c         2200 g         2823 t
ORIGIN      
        1 tcatgattaa agtagttatt gcagaccgta tgggtaaagg acaaaatgtg gccaaagggg
       61 tagaagcagc tggtggaaaa gcagttgtcg taccaggaat gggggcagat atgcgtttgg
      121 gcgatgtgat gcaacaagag catgcagata tgggtatctc tttttgtggt agtggtggtg
      181 caggtgcatt aacagcagca acaaaatatg gctatccaga acgacatggt atgcgttcga
      241 ttgaagaagg gatcacagct atcaaggatg gcaaaactgt tctaggtttc ggctttatgg
      301 atcaagagga attagggaaa cgtctgacag aagaatttat caaaaagcat ggaaaataga
      361 aagagggaca tgaaatgagc gttcaaaaga caattcagca aaaagttatc gtctccggta
      421 aaggggaaac aaagcaacat gcttttgcat cagcgttagc agaaattcaa aacaaaataa
      481 tggaacaatc aaacgtatta ttgcgaatcg aaccagtcaa tgtggatatt ttaaaggcgg
      541 tcgaaaacaa atacacagaa cgctttctat ttttcttctt cccacggcaa cgtattagct
      601 atgaagtcga actctctgtt actgtggctg taacagaaat aaaattggca gacatatcgt
      661 tcagccagca aatgactgaa gatccaaatg ggattgccat tccatttgta actaaaaaaa
      721 tctaattttg aatcataagg gggaaagaat aacgtggatt tcgtaatcat tctagtcaaa
      781 tcggtcatta tcgggggatt acttggtttt gcagcatctg tcggggcagc tcgaatgttt
      841 catgcaccta atacacaagg tttaggagct tttagaacat taggcgaaat gaatgcctgc
      901 atgggtgatc cagcttctca tttttcattt ggtttaggct ttttctttaa tgcatgggct
      961 tcttcagttg gtgctggtgc attcacacaa gatgtgacgc atcgaatcat tcccaattgg
     1021 gcagcggcag cacttctagc aaaaaacaaa gatgtcaccc agaccatgca tgatccgaaa
     1081 aaaatgggga tcattggagc ggttatcggg gcagtggtgg tagccttctt aaacacaaca
     1141 gctgcagcaa ttccagaggc gttacaagtg acggcggttt ccgttttggt accagcagcg
     1201 acgattctga tcaatacagt aatgccagtt attttttggt tggcagcttt agacgcaggg
     1261 aaacgaactg gtttttgggg cactcttttt ggtggtatcg cgcaattgat tatgggaaat
     1321 gcggtacctg gtgttgttct aggaatcttg attggtaaag gcgtcgatga gagcggttgg
     1381 aatcgcgtca cgaagagcat gatgatcgct attgttttat tatttatttt cagtggattt
     1441 ttccgtggat ttgatatgga aatgatcaag agcttccagc ttcaagtgcc taattggctt
     1501 gataacttgc acaacatctt tagtgtgaaa taggaggaga atcatggaga caacagaaga
     1561 acaagcaaaa gtattagaag caaagcggca aaagagcttt tggtttgcag actggtcttt
     1621 tccgctaatc gtaggaatta tgtccgctgc tgtatttgca ggaacacaca tgtatgtgat
     1681 gtatggtgtg ggtgcattca atgaagtttc gatcgtggcg atgttaaaag ccgggctgga
     1741 tggcggatcc tatggcgcag cggcagcgtt tggtgctagt ttcttatttg ccagaatttt
     1801 agaaggctct ttagttggga tcttggactt aggcggttcg atcctaactg ggatcggaat
     1861 tggggtacca gcaattctct taagtatgaa aatagcggca ccaatcgata attttgcact
     1921 ctctttattg actggtttgg ttctaggttt gattgttggt ggcatcattg tcttgattcg
     1981 taaatttacg atcaatcaat caaattccac ttttggtgct gacgtcatga tgggggcagg
     2041 aaattcatct ggacgttttt tgggtccatt aattatatta agcgcgtgtg cagcatctat
     2101 tccaatcggc attggcgcta cattaggagc actaggattc tatgcttgga aaaaaccaat
     2161 tgctggaggt gctattttag gagcaatgat tttaggagca atcttaccgg cagtaaatta
     2221 atagaaagag ggtcattaaa tgtttgatac tttgattaaa aatggaaggt tagttgatgg
     2281 atctaaaata gaggtcgcta tagaaaaagg catgattaaa gcagttgcag cagaaataaa
     2341 tcagccggcg caagaagtag ttgacttgga gggaaagtat tatttatccg ctgggtggat
     2401 cgatgatcat gtccactgct atgaaaagat gaatctttat tatgactatc ctgatcagat
     2461 aggggtcgaa aaaggtgtga cgacggtcat tgatgctgga actacgggag cagaaaacat
     2521 ccgtgaattt tatcaattga cgaaatcggt caagacaaat gtgtatgcgc taatgaatat
     2581 ctctaaatgg ggcattgtca aacaagatga acttgctgat ctaacaaaga ttcaagaaga
     2641 tctagtaagt actgcgttag cagaactgcc ggaatttatt gtcgggatta aagcgagaat
     2701 gagtaagaca gttgttggtg aaaatggcat taagccatta gaattagcta aaaaaatcca
     2761 gacagaaaac aatgatctgc cattgatggt tcatatcggt tctgcacctc ctgagttaga
     2821 agaagtattg tcaagaatgg aaagaggcga tgtattgacc cattgtttta atggaaaacc
     2881 aaacggtatt ttagatccaa agacagatca aattaaaaac tttgtctggg atgcttacaa
     2941 taatggtgtc gtctttgata ttggacatgg gacggatagc tttaatttcc atgtggcaga
     3001 aaccgcacta aaagcaggaa tgaaagctaa ctctattagt acggatatct acatccgcaa
     3061 tcgtacaaat ggcccagttt ttgatctagc aactacttta gaaaaattga gggtagtcgg
     3121 ctattcttgg gaagaaatca ttgaaaaagt tacagcagtt cctgcacgga attttcattt
     3181 gaagaataag ggccagttag ctgtcaatta tgatgcagat ttgacgatat ttgagattgt
     3241 tgaaaatgaa aaggaattga aagattccaa cggatttgca cgtatagcta aagaacaaat
     3301 tttaccgatt aaaacaatta ttggaggcgt catatatgac aattagctat ggaaagtttg
     3361 atttaaaaga agtcattaat gcatcgggaa aaatgacgat cctaggggtt tctaaggtat
     3421 ctgaaagtgt cctagcagca caacgatttg gaggcgagca tttttttgag atgagtgatc
     3481 ttgccatcaa aaccggcgct tatctttctc aattactgaa agtggaagat gcgcaaatcg
     3541 tttcttctgc ttcagcaggg attgctcaat ctgttgctgc tttaatcgga cagggcgaca
     3601 tgtaccatgt ctatcatccc tatacagaaa aaatcgctag acgggaaatc atcataccta
     3661 agggacataa tgtagattat gggacaccgg ttgaagtaat ggttgctcaa ggtggaggaa
     3721 aagtcataga agctggctat gcaaatatgt gtacaccgga acatgtttcc atgatgatta
     3781 cggaaaaaac tgctgcgctt ctatatatta aaagtcatca tgcagttcag aaaagtatgc
     3841 tgacggtggc agaaatggta gaagttgcca aagctcatcg tttgccattg atcgttgatg
     3901 cagctgcgga agaagattta ttcaaatata gcgaaatggg cgttgactta gtgatttact
     3961 ctggggcaaa agcaatcgaa ggtccgagtg ccggactggt cataggaaaa aagaagtata
     4021 ttcaatggat tcggctacag tctaaaggta ttggccgagc aatgaaaatc ggtaaagata
     4081 atattttagg ctttacccag gcactagaag attatttaaa aaatgggagc gaatccggtg
     4141 actccatgaa gcaacgatta gcgccttttg ttaacgcaat caatcaactt cctgaccttt
     4201 cagcgaaaat agttcaagat ggtgccggca gagatattta tcgagcaagt gtaacgatta
     4261 atggcgataa aagtgcgaaa gaagtcattc aggaattaaa agcacgaaat ccagcagtct
     4321 acacccgaga atatcaagca aataacggaa tcattgagtt cgacattcgt tccgtcagcc
     4381 aaaacgaaat ggaaaaaatt ataaaaagat tgagagaaat catgtaggag gcttttaaat
     4441 tgtcagttat cccaaattat tataaaaatc gtgtatgttt aaatgtatta gctcattcag
     4501 ttgaaaatgc aaaagcatgc tatgaagcag cggaagggca tgtcgtacta ggtgtgctat
     4561 cgaaaaatta tgctagcgat gaagcagcaa tcgaagatat gaaaaggtac caagacgcaa
     4621 ccaataatgc gttgtcagtt ggattaggcg ctggagatcc aaaccaaagt cagatggttt
     4681 cgcgtttatc tggggtttta caaccacaac atgtcaatca agtatttact ggcgtgggga
     4741 cttctcgtgc tttattgggc caaaatgata cagtcatcaa tggattggtt tcaccaacag
     4801 gtaagatcgg ctatgtcaat attgctacag gtccgttaag ttcacaagcg ccagcagctg
     4861 aagtgcctat tgaaactgcc attcaaatgc tgcaagacat gggaggtact tcaatcaagt
     4921 atttcccgat gaaaggctta gcacataggg aagaatttaa agcagttgcg gaagcttgcg
     4981 caaaatatga attctattta gagccaacag gaggaattga tctttcaaac tttgaagaaa
     5041 ttctgaaaat tgcaatcgat gcaggtgtaa aaaaagtgat tcctcatgtt tatagttcaa
     5101 ttatagataa agaaacagga gatacgcgta taggagacgt aaagatacta ttaagcatgg
     5161 taaaaaaaat tattcactaa ctcattagaa aataagaagc ttattccaga atgaaattaa
     5221 atggaataag ctttttataa ttaattacgg aaattgttaa tgtagataat aatttagatg
     5281 tacgtaagca gaagtacaga aatgtaagat tttccaatat tattttattc aacataataa
     5341 atcgtatatc aattttttta gcgaaatttc tatttattcc aagaatttca gttattacgg
     5401 aatcattttg tgcttaataa aaagaaaccg tttttatata ctataaatgt agaaaggcgg
     5461 taggaaaatg aaaaaaatta ttgcaatcac atcctgtcca gttggtattg cccatacata
     5521 catggcagca gaaaacttag agaaagcggg gaaagcagtt ggcgccgagg taaaagtcga
     5581 aacacatggt tccatcggta ttgaaaatga attgactgca agagatatag aagaagcagc
     5641 tggtgtaatc atcgcagcag atactaaaat agataaatca cgattcggcg gtaaaccttt
     5701 gataaccgta ggtgtgcaag aaggaatcca ccatgcagat gagttggtga gagatatttt
     5761 agcaggaaaa gcgccagtct ataaaagtac ggaggcaatt atttcttccg aaaataaaag
     5821 tgaatcagaa aatttaggta aaaaaatcta taagagtttg atgaatggcg tttcgtacat
     5881 ggtacctttc gtagttacag gagggttatt gatcgcgatt tctttaacat taggaggaac
     5941 agctacacct gaagggatca agataccaga aggaacgatt tgggcaacga tgaatagtat
     6001 cggaagtatc gctatggggt tgatggttcc aattctatct gcatttattg cgcaaagtat
     6061 tgcagatcgt ccaggacttg ttcctggatt tgttggtggg atgttagcgg caaacggagc
     6121 attatatggt agtgatgcga atgcgggatt cttaggcggg atcatcactg gatttttagc
     6181 aggattcatt gctttaggaa tcaagaaagt gcgagtaccg aaagctttac aatcgatcat
     6241 gccaattatt gttatcccaa ttttaggatc attggcagtt ggttttgtgt ttatctatgt
     6301 tattggagaa ccagtagctc ttctatttag ttcattgact cactttttag cagggatgca
     6361 aggaggaagt gaaattgttc tagctatgat cttaggagcg atgattgctt ttgatatggg
     6421 ggggccgttc aataaagtag cctttttgtt cggatctggc ttgattgcag aaggcaatta
     6481 ttccattatg gggccaattg ctgtagctat ctgtatccct ccattagcat tggggctggc
     6541 atcattgatt ttaaaaacca aatttacgaa aacagaacga gaagctggta aggcgtcctt
     6601 agcaatggga ctgtttggga ttacagaagg cgctattccg tttgcttcaa cagatccact
     6661 acgggtgatt ccaagcattg taatcggggc aatgacagga agtgtgatcg cgatgctttc
     6721 aggagtgaca gatcatgtag ctcatggcgg tccgatcgta gcggttttag gagcagtgga
     6781 tcacgtagcc atgttttttg tagcagtgat tgctggtatt ggtgtaaccg tgctaatgtt
     6841 aagaatcctt aaaccgacat tacaacaaac tgcggttgct gcagcaaatg gtgtgacagt
     6901 agcagatcta tcagagactg aaacaagtga tgtttatacg aaaactgaaa cttttgattc
     6961 tatcacggat attatgtcag atgaagcaat cgttttaggt gaaacagcaa caacaaaaga
     7021 cggagtaatc gaagatttag tccaacgttt agaaaaaaca gcggcgatca cagataaaac
     7081 cagatttaaa gaagtcatct atgatagaga acatgaaagt acaacgggaa tcggtatggg
     7141 gattgctatt ccacatggaa aatcagctta tgttcttaaa ccaacagtgg catttgccag
     7201 aagtcaatct ggggtagatt ggcattcttt agatggaaaa cctgcacata tgatctttat
     7261 gattgctgta cctgaaaaca gccaaggtga tatgcacttg aaaatactgc aacgtctatc
     7321 acgtaaattg atggatgatg acttccgtca agcactgatg gaagcgccag ataaaacaac
     7381 agttcaccaa ttattaagcg aaatgtaaca taagatgaaa aatcgaccgt gatgagacat
     7441 aatagatgtg ttatcatgat cgatttttta tttagaaggg agtttctgca cattatgaat
     7501 cagcgtcagc aatctttgat ctatcaatta agtgagaaca ataattatat aacaacaaaa
     7561 aagctagcag agcatttcca tgtgtcagaa agaacgattc atagcgattt gaataccatt
     7621 gaaacttggt tgagagacaa acatctgcct ttaatgtttg aaagaaaaaa aggtacaggt
     7681 atcttgttga atggtctgcc tcacgaaagg gaacaactga aacgagcgct taatgaagag
     7741 actcaaacag aaatggataa aaaacagctt cgtcagttat tgattttcca tctgctagta
     7801 gcaaaagacg gatttagtct tgaagaactt tcagaaaaac tatatgttgg aaaacgtacg
     7861 attcgtaaag aattgacaga gttgcaaagc ttttttgagt atcaccattt acagctagta
     7921 tccaaaacaa aactaggaac ttttcttaaa ggcgatgaac aagaaaaaag acagttgctt
     7981 gttaagacat tacgaaatat gcagaaagaa gatccacaaa gtccgtcgtt gaaagaattc
     8041 ttcgctaaag atacattaaa agttatccaa catacattac aagatgtttt ttatgaaaac
     8101 catttagaac agccgaatgg actggctagt gtggagattc acatctactt tatgctcgag
     8161 cgaatgaaac agtaccagaa agtgaaatta agtaaagacg aaaacgaagt cgtggaacac
     8221 acccaagcac aaaagttaag tagtcaaata ttagcaaaac tagccaccat ctatccaatt
     8281 gaattttctc ctgatgaaat caattatctg gctttgcgta tcgccaatct ttttacaaat
     8341 tcacaagcag ccacacgttt tcaaaaggaa tctagcgcac ttgctgatca tttgatcgaa
     8401 caagtggaac agtttctagg ctattcatta aaagaagatc agctactaaa acaaaattta
     8461 aggtctcatt tatcttcaac ttattttcga ctccattatg gtttgcagat ttctaatcca
     8521 ttgacgaaaa atgtgtttag tacgtacaca caacttttct tagttttaca gttaatttta
     8581 gaagattatt ttgaaaagga aaatttttat gttccacaag aggagacagc ttatttaacg
     8641 atccatttcc aagcagcgat cgagcgacac aaacatcaga aaagtagaag atatcaaacg
     8701 gttttagtta gtgaatattc aaaagctatg gctacttttc tcgaagcacg gctgaacaga
     8761 gaacttccag aattaacgat tatggatcta gttgaatata agccagaatt aaagagacaa
     8821 gattttccct ctgttgattt tatcttggct acgttaccat ttaaacatat aagcattcca
     8881 gtaatcgaga tctcaccaat gatcacagaa actgatctag cctatttgac aaaatatatg
     8941 ctggagcatg tgcctatcaa gaagaaaaaa acatttgatc tagcaagctt tactcatcca
     9001 tttctgatct ttcctcaact tgaacggaca gacccagccg atattttaaa ctttatggga
     9061 aacgtcttag tggaacatca ttatgtggag ccggaattca tcgattctgt cttggaaaga
     9121 gacagtcact ccagtactag agtagcacca tttgtaacaa tccctcacgg taatccgctg
     9181 tatgtgaagc attcgatgat ttctattgct acaatgaaag aaccagtcct atggcacggg
     9241 gaacagatac gtatcgtact gctattagca ataaagaaaa atgatttgaa acatgcagag
     9301 ttcaaaaaaa tattttctgt tattcactat ctagaaaagc agccagaaca aatggctcag
     9361 ttgatgcaat ccaatcatcc gctagaaatc ttaacgttgt tatctagtta tgaataagta
     9421 gcgattaaaa aactgccttt atatttataa taattctatc tatttatttg gaagtagaaa
     9481 actaaataca aaaaaagagg atgtggccaa agttgtggca catcctttta ttttgagttt
     9541 cttataattt tttatagttg cttacgatgt cttgactact ggtaaacggt gttcaagaga
     9601 caatttgtcg gcaataaggc acaattttgt aaaatatgga aagctatttt tttaacattg
     9661 tttcttagtg cttgaaaaat cattttgaat acttctttat acgatcgatg tacgttgatt
     9721 taagtctaaa acttttcttt tgattccttc tctgactttt ctgaattccg ttaatatttc
     9781 ttcttcgctc ccgacagctt gtgcaggatc ttttaggtcc cagtgaaggt gttcgtgatt
     9841 gtttggaacg gcagggcact tgtctttggc atcaccacaa ag
//
LOCUS       DACCQP010000093         9437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCQP010000093 DACCQP010000000
VERSION     DACCQP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.096"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            75..1379
                     /locus_tag="IVO87_002457"
     CDS             75..1379
                     /locus_tag="IVO87_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7450613.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="IVO87_002458"
     CDS             complement(1471..1836)
                     /locus_tag="IVO87_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7450614.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="IVO87_002459"
     CDS             complement(1833..2198)
                     /locus_tag="IVO87_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7450615.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="IVO87_002460"
     CDS             complement(2390..2638)
                     /locus_tag="IVO87_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450616.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="IVO87_002461"
     CDS             2637..2765
                     /locus_tag="IVO87_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7450617.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="IVO87_002462"
     CDS             2822..4516
                     /locus_tag="IVO87_002462"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7450618.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4598..4789
                     /locus_tag="IVO87_002463"
     CDS             4598..4789
                     /locus_tag="IVO87_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450619.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5167..6300
                     /locus_tag="IVO87_002464"
     CDS             5167..6300
                     /locus_tag="IVO87_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7450620.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6331..6651
                     /locus_tag="IVO87_002465"
     CDS             6331..6651
                     /locus_tag="IVO87_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450621.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6680..7144)
                     /locus_tag="IVO87_002466"
     CDS             complement(6680..7144)
                     /locus_tag="IVO87_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450622.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7273..8522)
                     /locus_tag="IVO87_002467"
                     /pseudo
     CDS             complement(7273..8522)
                     /locus_tag="IVO87_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8551..8868)
                     /locus_tag="IVO87_002468"
     CDS             complement(8551..8868)
                     /locus_tag="IVO87_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7450623.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8894..9214)
                     /locus_tag="IVO87_002469"
     CDS             complement(8894..9214)
                     /locus_tag="IVO87_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7450624.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3044 a         1860 c         1684 g         2849 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc
     4441 acgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 cattttttca acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6421 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6481 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6541 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6601 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agatttgaaa
     6661 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6721 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6781 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6841 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     6901 gatttggtgt caaatcaggg cggttatgga agtcgttttc aataacgccc gctcctccga
     6961 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7021 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7081 acttcgcact ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7141 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7201 aaattagcaa tgacaacaat agtgatgtaa aaaaaacgga aaacaatcac ttgttctccg
     7261 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7321 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7381 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7441 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7501 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7561 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7621 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7681 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7741 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7801 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7861 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     7921 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     7981 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8041 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8101 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8161 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8221 ttttcggtag ccgttagcta aatgataagc aagaaagcaa tcaagacaga aagaatggca
     8281 aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag
     8341 ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct
     8401 aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt
     8461 gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc
     8521 ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat
     8581 gatctctttt gctaaatctg taaaagctat ggatgtcatc agatgatctt gactgtggac
     8641 tgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg
     8701 agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt
     8761 gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc
     8821 aaacatgatc agacccatga tggcttctag atttttttga tcttccattt attgtatcct
     8881 ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat
     8941 tcatcatgcc atagtcagcc atattgatta tatctaaagg aatgccttta ggtgctagct
     9001 tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat
     9061 ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca
     9121 ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag
     9181 ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta
     9241 cacttgtata taagcaagta gcgtgccaac tttgaaagcg ctttaaaaag ttgatatcat
     9301 cacatttaag gtctatacca agttgcatac acactaaaaa agaaagtgca cactaaacac
     9361 acaaattaat ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa
     9421 acttgtcaca acttttt
//
LOCUS       DACCQP010000094         9417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCQP010000094 DACCQP010000000
VERSION     DACCQP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.097"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..368)
                     /locus_tag="IVO87_002470"
     CDS             complement(<1..368)
                     /locus_tag="IVO87_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7450625.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
     gene            complement(365..607)
                     /locus_tag="IVO87_002471"
     CDS             complement(365..607)
                     /locus_tag="IVO87_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP7450626.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1153..1683)
                     /locus_tag="IVO87_002472"
     CDS             complement(1153..1683)
                     /locus_tag="IVO87_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7450627.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1865..2521)
                     /locus_tag="IVO87_002473"
     CDS             complement(1865..2521)
                     /locus_tag="IVO87_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7450628.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2648..3121)
                     /gene="dfrF"
                     /locus_tag="IVO87_002474"
     CDS             complement(2648..3121)
                     /gene="dfrF"
                     /locus_tag="IVO87_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7450629.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3540..3758)
                     /locus_tag="IVO87_002475"
                     /pseudo
     CDS             complement(3540..3758)
                     /locus_tag="IVO87_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3789..5003)
                     /locus_tag="IVO87_002476"
     CDS             complement(3789..5003)
                     /locus_tag="IVO87_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7450630.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5344..5586)
                     /locus_tag="IVO87_002477"
     CDS             complement(5344..5586)
                     /locus_tag="IVO87_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7450631.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5618..6175)
                     /locus_tag="IVO87_002478"
     CDS             complement(5618..6175)
                     /locus_tag="IVO87_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7450632.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6188..6376)
                     /locus_tag="IVO87_002479"
     CDS             complement(6188..6376)
                     /locus_tag="IVO87_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7450633.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6389..6955)
                     /gene="amaP"
                     /locus_tag="IVO87_002480"
     CDS             complement(6389..6955)
                     /gene="amaP"
                     /locus_tag="IVO87_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7450634.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            8185..8658
                     /locus_tag="IVO87_002481"
     CDS             8185..8658
                     /locus_tag="IVO87_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7450635.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            8667..8894
                     /locus_tag="IVO87_002482"
     CDS             8667..8894
                     /locus_tag="IVO87_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450636.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2879 a         1847 c         1368 g         3323 t
ORIGIN      
        1 atatacgatg tactaggaat aatttcttca taaatcttgc cataagtttc atatatttta
       61 tccatagcac caatatgctc cacaaccaaa tatttataag acggtatttc tttatacaga
      121 aagccctctg gggtaatggt tctttgggga atagaacaga aataataaag ttttccattt
      181 cttctttcca taaaagcata ttttacccaa tttcctgatt gtgaaagata cgcttttttc
      241 aaactactgt taaactttct ccaaaactgt gtactaatct gaatattctt tttttgataa
      301 ttagtcagtt ctacttcctg cccaataact gcaaatgcgt ttaattcctt tatagaaaaa
      361 tttatcattt taccttgtga gcagtgataa cggtataact gtacgcattt accgttataa
      421 tacaattatc aatcgttata taccagtttt ttccttttct tgtgatttcc gcattagaaa
      481 ggctaatttt ttctttgcac cattcaatta cattttctac attcaaagat aagttctttt
      541 taattctaat aacgccaagt tcagttgtgt gaagtcttcc taagttttcc agtaattcat
      601 tttccatatt ttgatacctc atttttcagc aaatttattt gtatgtttta taccccaatt
      661 ataacattct catatcagca ctacaataaa aataattaag caccaataat cttattgaac
      721 atctccaacc gtaaatcttt cacaccatca gtgataaaca ctgagctgac agcaattacg
      781 acaactacca taattgcctg cacttaaatt tctgcaaaag aaaacgacaa tcaatttctt
      841 attttttgaa atcaactgtc tttctcatat ttactattca attttcatag tgatataggt
      901 acaatttata tcactactgt aaaaatgggg ttttgcctta taaatacagc gttttccgct
      961 tgttagtgta tagtgtttct tgttttttgt gccccccttg ttagataacg ggggctttga
     1021 acggctcgca agctcgccga accgccgaag cgaaccgccc ccttgcaagc atggcggaaa
     1081 aatcttaagg attttcccgc caacgtggtc ggatcgctcc gtcggtttaa cagcttttct
     1141 gtttctctct agttatctac gcatataaat caaatcggtt attccgttat aagtctgtgt
     1201 tttacaaagc cttaacttta tttccctgtc tatgttgccc aaaagacgaa ccccatttcc
     1261 taacagagta ggaataacag aaatgtaata tttatcaatc atatttttgc ttaccaactg
     1321 ctgaacaaga tttgctccgc cacaaatcca aatatccttt cctgcttctt gttttagccg
     1381 ttccaataat aaaacaggat tttcacttgt aaaatgaatt tgttgggaag aattgcactc
     1441 tttatgtgtt aatacataag ttgtaaattt attatatacc cattcttgcg gagaaagttc
     1501 agtaacaacc tgatgagaag tattccaccc cattaagaca gtatctatat ctttggtaaa
     1561 ttctgtataa gtgtcgatgt tttcattgtc atttccatga ccatttagcc aagctactcc
     1621 accattaccg tccgcaatat atccgtcaag gctcatggca ataaataaaa ctacttttct
     1681 cattttttct cttttataaa tacaaacagg aatttatttc tcttttttac tcataaggga
     1741 acaactgatt gctacaaata aacccattaa aatccatgga aatgctgctc ttataaaatc
     1801 actaaccata ctttattcct ccttaaattc caatttcctt agttctacaa actgaaattt
     1861 ttgcctatga atgtatcttg ttctttatac gcaaacaggc ttctgtgtgc ataatccaat
     1921 gtcttgaaaa cggcatttga attagtccac aaatatcttt attacaccaa taatcaatca
     1981 gccaaattgc tttttcattg tcgtttacta cattcaacga ttctaagtat ccttttcttt
     2041 ctttcggtat ttttcttttc aattcatcat aagataaccg ttcaagcatt ttctcagtgc
     2101 tttcccatac ttcgaaaata tataaatata attcttccag attaagctgt tttgaaaagt
     2161 ctgcaatctg ctgtttcatg agttcatttc ctgttgtaat gataggtgaa ttgatacgct
     2221 cttgataatt gcctgcaaaa aatacttgtt catcttcatt tatcactgta tgcacaacta
     2281 tatcttcaat gcggaaaata tgccaaatag aataagcaat cgtcttacta tgataaccat
     2341 cagcgtttat gaaggggatt gagttaaaat cttctctgca taattcttct ttgaaagata
     2401 ctaaggtttg tattaactga cttcgtaaag taagtaaagt atcaataccc ctcttataag
     2461 tatctttctt ttttatttgt gcctgcattg ttttattaag ttcagaccat tccttattca
     2521 tattcaatcc tctctcaaat tctgatttta tcgccccata caaactggaa tttgtcttac
     2581 aacaatttat atatgcataa aaagcaatat atttatgaaa acaccctaaa tccaaaattt
     2641 actcaattca ttttttcctt acataaaatg ttctcgtata tttcacttcc tcaccaagtg
     2701 tttcccctat caaaacttca aaatcattga tatcaaattc tggaaagaat gtatctccat
     2761 cttcaatatt taaatctact tctgtgatat acattttatc tactatttgc aaagcttcct
     2821 taaatagtcc atatccacca gatatgtata catctcgtcc tttagccaat aataatgcat
     2881 cttctaatga tttaactgaa actaaattat ctccttgata ctctgttgtg gtggaaacaa
     2941 caatattcat tctattaggc aacggatgac cgatttcttc ataagacttt cgccccataa
     3001 taaccacatt acccgttgtt aactctctaa attgcttttg ttctcccttt attttccatg
     3061 gtatattacc attcttgcct ataacattat tctttgacct cgcaacaatc aaacctatca
     3121 tctatttcat cctccataat atgatgtcgt acctgtgatt ccatcaagtg caccacatct
     3181 taacaaactc aaaattatct cgaactaacc agctcgacaa attccttttt tgtcttcatc
     3241 tgcattatat catattgtta taagcattgc catttctttt ctgcttttga aaagttactt
     3301 atcagcctat gttttgtaaa gggtgatatt cagcatttct cacgcaacaa gcccttgaca
     3361 aaccatagac taataagttt taaggtaatc aagcagaaat gaaatagtct gctgtttcac
     3421 ggcaacaaaa aagagatacc aacacactta tgtagtatct cttagtctag tagcaatatt
     3481 cttttgtttg caagcaggta aacatgggct gtttcttaat atctttttgt ttgggaaaca
     3541 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
     3601 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
     3661 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
     3721 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
     3781 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
     3841 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
     3901 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
     3961 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
     4021 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
     4081 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
     4141 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
     4201 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
     4261 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
     4321 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
     4381 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
     4441 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
     4501 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
     4561 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
     4621 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
     4681 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
     4741 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
     4801 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
     4861 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
     4921 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
     4981 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
     5041 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
     5101 gacaattaat aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca
     5161 aaaaataaac ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa
     5221 agatattttt tcataaggaa taaagaatac ttcatataaa atatataaaa aagaaccgca
     5281 gcgagtgtcg ctacggttct caaatttgtt cttcatttca acataagttt ttcttcaatt
     5341 tgcttataat ttacgtgcaa agaatgaaac gactaatact aaaatgattg caccgatcaa
     5401 tgatggaatg atagccattc ctgcaattgt tggtccccaa gttcctagta aagcttgtcc
     5461 aattgctgaa cctactaaac cagcaatgat gtttgcgatg atccccattg atgatccttt
     5521 attcgtgatt gctcctgcaa ttgctccaat gataccgccg acgattaatg cccaaataaa
     5581 tcccatttta aattcctcct aactgacttc ttacttctta ttgtacacgt ggttcgttat
     5641 tttctttgac tttttgtgca cctttgccta cagcagattt tgctttatct gtttggcgtg
     5701 aagcaaattg tcctgtggat tttgctgctt ctgttacttt gtcttgtact gtttcttgat
     5761 ctttttggta ttcttcttgt gttttgatgt ctgcaacatt cacattcact tcgataacat
     5821 ctaaatgtgt catattgttt acttcagtac tgatcaattc tttgatttgg ttgtaaatat
     5881 cttcgatgtc tttaccgtat tctacgatca catctagatc cacagctact tgtttcttac
     5941 ctacttctgt attgatacct gctgttacgt tatctgtgtt gactaatttt tcagccatat
     6001 ttgagaaaaa tccgccatct aatgtcaata gaccatctac atctttcaat gcataaccga
     6061 tgattttttg gatgactttg tcttcataag tcaattcacc attcatcatg cggttttctc
     6121 ctttttcatt tgccaaaact gatccatttg ttgtattcat gtttttgttt tccatctttg
     6181 tttcctccta tgatttaaaa aattgatcaa ctattcctga ctgtttcaaa taaaaaccga
     6241 ttcctgcgcc aattgctgtc atgattatga tcaacaatgt cttgaaaaat ccaatcgtta
     6301 accataaaac agctagcagt agtccaagac ccccaaagat aatagggagc ttgtatgctg
     6361 aaaatagttc tttcattatt tggccctcct attccactcg agagtgcttg tagtctcttg
     6421 tatcttcttg ttcttcataa ccgctgtatg tgactgccac tttgaccgtt tcttttgttc
     6481 cgagtacttt agttacttct tcttcgatat cttgcatcaa tacacctgtt ttccctacta
     6541 atgaagaagt tcgtttcaag tctccgttga catggatatg gattcgattt ttcgttgaac
     6601 gtatccgtac tttaggtgaa tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc
     6661 cttcgatcgc ttttttgtcg attgttagtt tgccatcttc ccgtttcata acgaaagttc
     6721 ctcttgactt tggatagaaa agaacgacta gcaagatgat aatagcaaga acggcaaata
     6781 ctacagctgc ccagaaaata tattgttgca tgtagtaatt cgttaaagta aagaagcgaa
     6841 atcgttccaa ttgttcaggc ataatatacg gcgcattgat caacgcaatc gctagaaata
     6901 ctgaaagtag cagtaaactc atgataacac cgattatttt taacccctta ttcattttcc
     6961 acctcctata aacttatttt taccctgtaa ataactctac cgacaatcac agtatagata
     7021 gcgaacagtt agcatagcat cagtaaataa agcgctacca aacattgctg gatcataacg
     7081 gcgttttatt gccagtctga tactgaaata acaattgctt acttattctc tatttacttt
     7141 tgattgctgg ctatacttaa aggatattat tcaagattaa aaattgcaaa gattatgctc
     7201 agttattatt acaagattct atttatttat taaatttttt cggagaatta aaaaatacaa
     7261 taaataactt tagttacttt aatgcgttct tactttattt ttatattatc atttttctta
     7321 ttcacttctt tggtttatac tattcttata aataaaaacg aggtatgtcc tatgatgaaa
     7381 agattgtact attcattgat cataacgatt ggctatttga ttgtatcaaa tctaggaaat
     7441 atggtatttg gtatcagtaa agaattcagt tggactacta cattatggga atctctattc
     7501 tttttcatct tcgtattttt gttacaaaat tatcgaaaaa aataaacatg tgccatctat
     7561 ctgttggcac atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc
     7621 tacttcttct taatacctct aacacttttg atttataaac taatccaatg catctacttg
     7681 cgcttattaa gctatttcag ctagatagta ccaccccatg cgtcaatgct tgattttcct
     7741 tttgtataac attgtataag caataaagga caaataagtg gtgattacac caaaccattg
     7801 aataagaaaa attctgatac gtaaataaac atgaacttga aaaaattaat gaatcggtta
     7861 gctactacgt agttgtcttt tttctcatac agttctgtta acttacactt ctcttcgcta
     7921 gataataaaa aatcctccaa ctgtattgtc caatataatc ccaggcatct aaactagcat
     7981 tatagaagag acacacatag aatctaaaat aactggtttt aaatgtttct tacaatcaaa
     8041 aaaagctgtt aaatgcactc cacaccttca tactctatca ttttttataa acgatatatt
     8101 tttattgttt ttcgataaaa aatagttgtt ttacaataat tattctgcta tattttagct
     8161 atcaaagata aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt
     8221 ttaggtataa gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca
     8281 agcgaaaaag gattcaattt tgattggcag accacattat ttgtttctgt catttacttt
     8341 gcccttctcc tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa
     8401 gataaccaag ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt
     8461 ttgtttattg gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta
     8521 gacgatgctc caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta
     8581 tacacattaa caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat
     8641 catgatttga cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag
     8701 ctcaaaaaaa aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca
     8761 tctctattct aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct
     8821 gccaagtttt ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta
     8881 taccagaaga ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga
     8941 tgtgtgagac ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat
     9001 accgttttat ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc
     9061 atgtactatc acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt
     9121 ttagtataat actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc
     9181 atgtagtatt acaggccatt ctgtttcata ccttgaagtg cttaaaactt cagctaatgg
     9241 cccattgtgt taactcttac tccattcttt tttagtaagc tcccatatat actgtaaagc
     9301 cttctatata ttatgtcatg ttgcttttta acatttacta tgtagttttg ataaaatgat
     9361 ttgaataaca gtgatggaca attctttttt atttttaaca ttattttgat aataatg
//
LOCUS       DACCQP010000095         9257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCQP010000095 DACCQP010000000
VERSION     DACCQP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.098"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            632..1222
                     /locus_tag="IVO87_002483"
     CDS             632..1222
                     /locus_tag="IVO87_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP7450637.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1249..2559
                     /locus_tag="IVO87_002484"
     CDS             1249..2559
                     /locus_tag="IVO87_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7450638.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2556..3275
                     /locus_tag="IVO87_002485"
     CDS             2556..3275
                     /locus_tag="IVO87_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7450639.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3290..4375
                     /locus_tag="IVO87_002486"
     CDS             3290..4375
                     /locus_tag="IVO87_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7450640.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4511..6043
                     /locus_tag="IVO87_002487"
     CDS             4511..6043
                     /locus_tag="IVO87_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7450641.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6221..6442
                     /locus_tag="IVO87_002488"
     CDS             6221..6442
                     /locus_tag="IVO87_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP7450642.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            7024..7641
                     /locus_tag="IVO87_002489"
     CDS             7024..7641
                     /locus_tag="IVO87_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7450643.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            7638..8168
                     /locus_tag="IVO87_002490"
     CDS             7638..8168
                     /locus_tag="IVO87_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP7450644.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(9034..>9257)
                     /locus_tag="IVO87_002491"
     CDS             complement(9034..>9257)
                     /locus_tag="IVO87_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7450645.1"
                     /translation="PGTPSQRPLNENSNGILRRNGLPKSIDFREVNQTFISSVSNQRN
                     HIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         3283 a         1010 c         1781 g         3183 t
ORIGIN      
        1 ttgaaggcga tttaagctat atattagaaa atgagatatt tgtaaacaaa acaaggagac
       61 ttttgttggg acgctgtcgt acttgtattc aaaaggcagt tagatatgct agttttaata
      121 aagaatttga tttaaactcc tatttgtcta agatacttat gaacgatgag ttgattgcct
      181 gtattcactc atacccatat aaacaaaatc caattaaaca acgaatattt aactattttt
      241 tactaaacca atccattatt ggggttaaaa cattagcata tttaagcgga atgggtagaa
      301 ataggagatt ttaatcataa aaaactgtac tatagtgaat ttttggagat ttatattcca
      361 gttatatatt aatgattgtg atacttgtac tattgtaaaa attcgttagg tttaaataac
      421 tggaaatatc ggaaaatggg cattatacat cacggacatc ccttcttttg gaaaaaatgt
      481 tacgattttt ataagtaata ttgtatccgg tggaatcaac gatgaagata acatatccat
      541 cgttgtagat tcagtagcat tgaatgatgc tcttgttgca agaaatccga ataaaattgt
      601 taagtataaa ttgaattaat aggagatatg aatggaaatt agcagtaaag aatatttaaa
      661 agcggttctt cgtatggaaa agaatctata tatagaatct aaaggacaac acacaaaatt
      721 actaaaaata atgaaatcac atccggacta caacgcttgg cggtatactc gtcgaatgag
      781 aattacatct tattattatt caaagagaaa gaaaaatatc atttatgcga ttatgtatat
      841 tgtgtcttgc agaagaatga atagtttggg aagaaaatgt ggtatagagt gtggtgaaaa
      901 tgtatttgat gaaggtgtaa gattttacca tacacaagga attgttatta atggtaacgc
      961 cataatagga aaaaattgtt acttatatgg gaataactgt attggaaatg atggaatcga
     1021 tcctaagtgt cctgtaattg gaaataatgt acgtgtatgc gtgggtgcaa agattattgg
     1081 taatgtaaaa atagctaaca atgtagttgt tgcggcagga gctattgtta tcaaagattg
     1141 tctagagaat ggtgcaattc ttgcaggtgt accggcaaaa ataatagggt atgttaattt
     1201 aacaaaagta tttttagaat aatttttagc gaaagaaggt gaataacgat gcgtatttca
     1261 ataaaaagat ggtcaggagc agattggaca ttgttcacgt tggcattttt tgcaatctcc
     1321 atagttcttg tatatacttt taacgcccca agagcaattt tatatttggg agatataatt
     1381 aatatctttg tgttattaaa tgcacttagc tttagacgca gaaaaggaat atatgctagc
     1441 aaaaatgaga tgctgatagt tttgttttta ctagtttcta tttgtagctc agttattaat
     1501 ttagaaaata taggattgat tatctggggg atacgaaata acactcgtat gttcatagta
     1561 tatataaatt gtgcaacata tttaacaatt aaagattact ataaagtatt cgctataatt
     1621 gaaaaattat tctggatttc actgccactt tgtttaattg aaagattcat ggtcacctat
     1681 ccaactggaa caattgtagg agatatgata ggtggagttt tttggaatta ttcaggttgt
     1741 aatatgccat tgaatttaat tttgtgtgtg acagttactg ttgttacaat cggatatttc
     1801 aagggagaaa aaaatttcat ccattttatt ttatgtgttt gtgcagcact ttttatgtct
     1861 gcattagctg aattgaaagt ttttattcct gaattattta taatatttgt tgcaatagta
     1921 ttcactttaa aagttgattt taaagtagtg atcaagatat tatcatgcgt aatacttgtg
     1981 tcgatagtat tatccttttt tataacaata tttattacat tgaacgataa tggacaaagc
     2041 tatgctgata atttcacaat tcaaggtctt ttagaatatg cgtcaagaga ttctggatat
     2101 gatggtgttg gtgatttaaa tcgaataact ggtatctcta gagtagtaga tgaaatattt
     2161 aataataatt ttagattagt cctaatggga gtaggcattg gcaatgcaga gtttaccaat
     2221 ttttttgtca gtgagtttta taaatcgttt ggtaatcttc attatcagta ttttcatcat
     2281 atttggttat ttatagaagt tggaatatta ggagtttcga tattcctatt ttttataata
     2341 aagacatttt tcaatgcaaa aagatctttt atgaaggata aagatgcaag tcttgttaaa
     2401 gttattgcaa ttatgtcggt attctttttt gtgtataaca caacacttag gggtgaaaca
     2461 acggggccac tgatttatat gattttagcc atgcctgaag ttgtgaaaag gaaagagcat
     2521 tattatagtg aaaaaaatac aaaaggagag atattatgat tcctaagaag atacattact
     2581 gttggttcgg gggaaatcca cttccgaaaa aagatagaaa gtgcatagaa agctggaaaa
     2641 agtattgccc tgattatgag attattgaat ggaatgaatc aaattatgat ttaaataaga
     2701 accgttatat ggccgatgca tataaagaac gtaaatgggg ttttgttcca gattatgcaa
     2761 gatttgatat tatttataat catggtggat tttatttaga tacagatgtt gagctaatta
     2821 agtcgttaga tatacttcgt gaaaatcatg cctatatggg atttgaaggg gaaatctggg
     2881 ttaatggcgg tattggtttt ggtgcagaag aaggacattc tgttattaaa ggtttaaggg
     2941 atatttacga ttcattaagt ttttatcaaa atgatggtac attgaacttg aaaccgagtc
     3001 cgtattatat tacagattat cttgaatcta ttggattggt tcgcgataac agcgaacaaa
     3061 taataaatgg cctgcatatc tatcctacgg attattttgc tgccaaggat tatgatactg
     3121 gaaaaattta taaaactagt aatacaataa gcattcatca atataatgca tcttggaaaa
     3181 cacctaaaca aaaatttgtt tatagattaa aatcaacaat tggacaaaaa aaatttaaca
     3241 ggttacttgc actgaaaaaa tttttacact tataaaggag aaaagtactt tgaagaaaat
     3301 ttgtttcatt agtggtgtaa tttctcgttc tgggggaact gaaagagtag ggagtattat
     3361 tgccaatagc ttgtctgatt taggttatga tgtcactatt ttaagctttt ggaatagtgg
     3421 agagccttat tttgggttag ataagacgat taaggttgat tatatgttaa atcctaaaac
     3481 ggagggaaaa ttatatagaa cttatttata tccggtttgg aaattgcaca agtatatatg
     3541 caaaaatgaa tttgatatag taattgatat tgatactttg ttgtctttgt atactacaag
     3601 ggcaattaaa ggaacaaagt gcaaattgat ttcttgggaa cattttaatt actggacaat
     3661 ggaaatgctg aaagaaaaga agagattcaa ggctaaaaaa ttgataaaaa aatatgcaaa
     3721 gaggcttgtg gttctcacag aagaagatcg tcaaaaacat attgatgaat atagtttatc
     3781 agaagatttt gttataacta tgccaaatcc gtgtatatct aatatcgagt acgaatatca
     3841 ttatgataaa aagagatttc ttgcagtagg gcgattagtg gaagagaaaa attttgctga
     3901 catgataagt gcttggaaaa ttgtagaaaa aaaagtagat gattgggatc ttatcattgt
     3961 aggaaaaggt gaattagaaa aagatcttcg ccagcagata aaagatttaa atctgcaaag
     4021 agtaactata gcagggcatt cagaccatgt ggaggaatat tataaatctg catcttgtta
     4081 tgtgcttagt tctgtttatg aaggatttcc tatggttata ttagaagcac agtcttatgg
     4141 attaccagtt atttcttatg attgtaaaac tggtccgaga gatttggtac atcataattt
     4201 taatgggatg ttggttgaag ataagaatat agaccaatta gctaagagca tgataatgtt
     4261 tactaagaat actgatttag caatgagaat gtcactgaac gcatataata atgtacaaaa
     4321 atttaatttg aaagaaatta caaaacaatg ggttgctttg attgaaaatg tatagttttt
     4381 tgtggttgtc caaacatgaa aattagcgta attaatcaaa gagctagaag aaacctatta
     4441 catttcaaga ttttagaaat tatatagtat taggtaacaa taaaaaatag aaagtttgga
     4501 gccatatgat atgataatta atcgtaaaca aaatgctaaa aggaatataa ttactggaac
     4561 aatttttaag ttatatcaga ttatcattcc cttttttatg cgtacactta tgatttatca
     4621 gatgggaatg gagtatgctg gattgaacaa cctgttcaca tctatatttc aaatgttaaa
     4681 tttagcggaa ttgggaatag gtgctgcact aactgtaagt atgtataaac ctatagcaga
     4741 agacaataga aaagagattt gtgctttact gaacgcatat aaaaagtact tttcaattat
     4801 tggatggata gtgtttacat taggattgtt atgcattcct tttttgagat ttttagttaa
     4861 aggttcgatt cctggaaatt taaatatgac agttttgtat ttaatgtatt tgataaatac
     4921 tgttttgtcg tattggttat tttcatatag aaagagtcta atatatgcac atcaaagaaa
     4981 tgatgtaatt aacgtgtcta tgctaattac ttttacattc caatttgtaa tgcaggcata
     5041 tgttctgatt ttcttaaaaa attactattt gtatttattg gcttctattt tgggacaaat
     5101 agttcttaac ttaatgtgtg ctgttgtttc tcaaaagatg tatcctgatt atactcctgt
     5161 tggtgagtta gagaaagaaa ctattgacga tattataaaa aaagtaaaag gattagttac
     5221 aaacaaggta ggcggtacaa tattacgatc ttcagattca attattattt catctttctt
     5281 aggtctttca gtacttgcga tttatcagaa ttattacttt attatttctg ccataatgag
     5341 tatatttgca ataatttatg aatcatgtat agcaggtata ggtaacagtc ttattgtaga
     5401 aagtcaagaa aaaaattata tagatttttg tgatattaca acaattatag gttggtttct
     5461 ggcaattagt tgtgcatgct ttattacatt atttcagccg ttcatgatta tctgggtggg
     5521 aagtgataat cttatgagta tttcatttgt tatatttatg atagttcatt ttttcttata
     5581 tgaaattgat cagttaattg ggttatacaa agatgctgct ggaatatggt acgcagacag
     5641 gtttagacca ttaattacag caatgataaa cattgtatta aatatcatcc tcgttaattg
     5701 gttcggatta tatggagttt tggcggcaac cattgtttca gtattgttgg ttgatttacc
     5761 ctggttatta tataatgttt tccaaaatat atttaaattt gaatcggcag gtaaatatgt
     5821 ttggaagctc ataagtttgt gtgtaatagc ttttgttgct tcttatttaa gttattttgc
     5881 ttgttcgtat gttaatatcg gaaatttcaa agtgataagt ttagtgataa atttagttat
     5941 atcggttgtc atatcgattt tagttattct tctgtttaat atccgtaata agaagtttaa
     6001 gtatattttg aaagttctta cgtcggtatt ggtaaaaaga tgagaagaga catcagaatg
     6061 atatataaag taactacaat ttttaggctg ccgaataggc gaacgatgta tagtttttgt
     6121 tccaacgaag actcttgttg aaattggaga tgatatgaag gagttacaat tttgactcat
     6181 gtttatgatt gatcggtttt aaaagtagta tggagaagct ttgggatcat cggagaggat
     6241 caaaataggt aataatgttt ttataggaat gaagtctaga atactaaagg gtgtacatat
     6301 aggaaataat gtagttatag gtgcaaatag tttagtaaac aaggacgtat cagacgattg
     6361 tgttgttgca ggaaatctag caaaagtaat tatgtcccta gatgaatatt ataaaaagat
     6421 aaacaatatg aagaatccgt aaaacttgtg aaactatacc gagaataata tgggaaaaat
     6481 attgatgaaa aagcattata tgagttttta gggctgttta cagatggaga tgacaatttg
     6541 tcgaagagtt gggagcaatg caaaatttag taggggatta taaacagact gacaaggtat
     6601 ttaaagaaca tgcacaagta tttcataata ttgaagaatt tttgaatact gtgagatagg
     6661 agttgctaca ttgagaaaag tatgtatttt tccaatgtaa aagattacga gtttgaaaac
     6721 gacaccataa tattattttt taagaagata ttatagagct taggaacgga agtttattta
     6781 aggatgttga ataaaatgaa aaataagatt aggtggatga aaaaggcaga tgatttttta
     6841 ctataattaa aaatattgtt actatgttat caatcgtata aaaatactta ctcgttatca
     6901 agacgattat ttcgaatatt ttaggaaaaa taaattgaat attagagaat gtgataatac
     6961 actctaatat gatcatgtgt aattggaagc gataagtgta tacttgatat tggagaaata
     7021 attatgataa tagtgattgc agaaattatt acagatatat tatataaatt atatgaaccc
     7081 tttgggtttg ctgttttatt gacaatttta ggactggtct attttatgta tgcaacagat
     7141 ggcattgctg caggtgaggg ctataaacgt atactaatgg aactttatct tcatttaaag
     7201 aaggatagaa aatatagaaa agtttgtgct ttgatattct atactgcgat gattttattt
     7261 acaacattaa tcaatcgaac aatttggtta aatcctttat ctgatgttat gggagactgg
     7321 tggatttata agtataatac aacaactgga acaaaagagt tatctacagc gtgtttagaa
     7381 aatattattc tttttattcc atatgtttat atggtaatgt ctattaaata tgaaaatata
     7441 aagaaaccca aaatatggtc ctcaatagta tatggaggaa aaattgcatt ttatttttca
     7501 gcaataattg aatttttaca actttttttg aggctaggaa cgtttcaaat ttcggatttt
     7561 gtatataata cgttgggcgg tatattgggt gtaatccttt ataaactaat tttgattaca
     7621 aaaagagatg gtgaaagatg agactcggat tgataataaa gaatatatta aatttaccta
     7681 aaacgatata cttcaattat aaatttcttc atatttcaga tgcgtggaga ttacctattt
     7741 tggtgcatcg taaagtacgg attacacgca aatcgagagg gaaaattgag atagtattac
     7801 aatctggaaa aatagtttat ttgggctttc cagtattaaa tactgcggga aatgaagaaa
     7861 cgattataag tattgaagga atactaagaa tacaagggcc agcgtttatc gcacaagggg
     7921 ctcatattga cataaggaaa aatgcagttt tagatattgg gtcaaagttt aattcgacag
     7981 gaaaaatgag tataatttgc tttaaggaaa ttcaattcaa agatggctgt acagtatcgt
     8041 gggatacatt atttatggat accgattcac atacgatttt tgaattgaat actcatagga
     8101 ttttgaataa aaataataaa atagttatag gtaaacattg ttggattgga gcaaaaagcg
     8161 tgatatagaa aggtgttcat ttagcagata atattgttgt tgcttgtaat tccacggtag
     8221 taaaaagtca tgaaaataat aatgagatta tagggggggc tccagttcga acgttgaaaa
     8281 gaaatacaag ttggtgtttg aacgagccaa agtgaaacca aataattata aaactatgga
     8341 agtataataa agtttttgag aaatttgaac gacaagaccc taaaaaaatc catggaccat
     8401 tattatggat ggacttccca aaaggaatag ccacttttga gcacgtaaat cccctaataa
     8461 gtggatcgac atcgcttgtg cagcagatga tactatcaag aaaggtgttt ataatgtaga
     8521 acttgaacta aatagatttg taatcaccca ttaggattgt aaagaatatg aataggatat
     8581 atcttaagat gaaaaaattt gcaaattcta atgtatattt attaagggca ctttgctgtg
     8641 tttttttcag agataagatg aaagaaagat ggaagtcttg gagtatacga aataaagaaa
     8701 aatattatat ctgtttaata agttgatatt gaacaaagga ctagtcggag tcagcaacac
     8761 ataagttgtg agtgatgctg actcgttttt ggcagaaaag aatcgaatag taagtaggtg
     8821 ttttgatgtt gcttgacaat ggagagagaa cccaagaaga ttactagcaa agcttataga
     8881 tgttactgta gatttttaac tccagataca gagtttgatg aatattctga ttatatcaat
     8941 aacactataa aaaaggaaat ttccttttca caggagaaag gtaaaagata ttttcatagg
     9001 agaatcaatg ttaattttaa attatatgat ttgtcaaatt aagctagaat aaaatgcttc
     9061 ttgtacatag ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat
     9121 atgattgcgt tgattgctga cactggaaat aaatgtctga ttcacttctc taaaatctat
     9181 tgatttcggc agtccattac gacgcagaat cccgttagaa ttttcgttta atgggcgttg
     9241 agaaggtgtt ccagggt
//
LOCUS       DACCQP010000096         9078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCQP010000096 DACCQP010000000
VERSION     DACCQP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.099"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            58..414
                     /locus_tag="IVO87_002492"
     CDS             58..414
                     /locus_tag="IVO87_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7450646.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            404..670
                     /locus_tag="IVO87_002493"
     CDS             404..670
                     /locus_tag="IVO87_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450647.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            829..1830
                     /locus_tag="IVO87_002494"
     CDS             829..1830
                     /locus_tag="IVO87_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7450648.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2200..2814)
                     /locus_tag="IVO87_002495"
                     /pseudo
     CDS             complement(2200..2814)
                     /locus_tag="IVO87_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3054..3299
                     /locus_tag="IVO87_002496"
     CDS             3054..3299
                     /locus_tag="IVO87_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450649.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3397..3618
                     /locus_tag="IVO87_002497"
     CDS             3397..3618
                     /locus_tag="IVO87_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450650.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3940..4620
                     /locus_tag="IVO87_002498"
                     /pseudo
     CDS             3940..4620
                     /locus_tag="IVO87_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            4682..5299
                     /locus_tag="IVO87_002499"
     CDS             4682..5299
                     /locus_tag="IVO87_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450651.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            6319..7488
                     /locus_tag="IVO87_002500"
     CDS             6319..7488
                     /locus_tag="IVO87_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7450652.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7961..8515
                     /locus_tag="IVO87_002501"
     CDS             7961..8515
                     /locus_tag="IVO87_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7450653.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            8769..>9078
                     /locus_tag="IVO87_002502"
     CDS             8769..>9078
                     /locus_tag="IVO87_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450654.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         3389 a         1201 c         1580 g         2908 t
ORIGIN      
        1 attcgaattt ttatgttata tactataata taaaatcaat atatgatata attgggtatg
       61 gaaaaaatat cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg
      121 ttggaaacat taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa
      181 aagaatggga tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg
      241 tacgaattaa gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc
      301 aatggaaggt atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt
      361 gaacttaaac atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt
      421 gattgaagaa aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa
      481 actcgaaact gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga
      541 gcttgcacaa aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa
      601 cgtatctttc gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga
      661 atttgcttaa aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaattagtt
      721 tagtgggcct ttttgtacta aatagataaa agttgtccta tagtgtaaaa gtcaataaat
      781 tttatacaaa aattacttta aagagacata aagagaaagg tacaataaat gaaaaccata
      841 aatctaataa aagtatttat agcatcacca ggtgatatag cagcaaagag gaatgaagta
      901 gaagaaacca tatatagatg gaatatagaa aatacagata atacaaaagt aattttgatg
      961 ccaattcgat gggaaacgaa tgcgattcct acttatataa ataatagctc tgggcaaagt
     1021 gttattaata aacaaattgt agaaaatagt gatatattat tagcgatttt tggccataag
     1081 ttgggttctc cagttgataa ttatagatcg ggaactatag cagaaattga ttacttttat
     1141 aaaaatagca aaggcgaggt aggggttttt ttcttagatg aaccagttcc acaagttttg
     1201 gctgaagagt atatagaagt taagaagtac agggactctt tagaagataa gggtttttat
     1261 ggaacatata gcaaagacaa tatcagaagg tttctcacaa aaaaagtgaa ttatttaatt
     1321 gagaaagcac aaaataattt agaatcaaaa cgctttgatt tagattctat ttcttacaag
     1381 acaccttcag taattaacaa aaaagttacc ggaactgata aagattttga tgacaactta
     1441 gatatttttg atccaataga atttgacaat gatgaaagat tgctgctaat atatgcgttt
     1501 atggatgaaa attctatctt ttcaactgat aattcaactt atagaaaagt agttcaatgg
     1561 gaaagtgata acaatttaag gagttacttg agtgatagag tatttgatgt actagaaaaa
     1621 ttaagtaaat cgggaatttt aaaggaagaa tacgattctt cttttgatga gtatccatca
     1681 cgctatattt tgatttcaga ggactataag aaatttaaaa ggtatttttt aaaaaatgaa
     1741 aaaaagatat ctgttattat ggagaaatac aaagctccaa tacaggctga tccattttcc
     1801 aatttggatg aatcagattt accgttttaa attttaaata gataatttca ttttttagta
     1861 gcagaggtat aaactttttt aatgtttgaa ttgaaaagaa atgctaatag gtcgacttta
     1921 tgtttctctt gtgcaatcgt aaatgcgggg gaccataaaa ttgatctatt atcgagtgtg
     1981 tataaaataa aattcaaatc ttattagttt acataagata cattatacta agttgtcaat
     2041 gttaaatgcc tttaaataag gacgatttat gcatcaatta tatttatgtt aactaaattc
     2101 tttttaacat aaatgtaatc aacttagcta gatgaatcta aaatgattgt ctgtattgat
     2161 gacaaagcta attaataatt acgtgattca aaaaagaaat catacaatta gttggtcgat
     2221 ttcttttttt attcggtaaa ttgtttgcct agtcacattg tagtctgttg caatctttga
     2281 gatagcaacc ccattctcaa gatcctgaac gatactccta tagacaaggc gacgttgagg
     2341 atctttggtg tctgcactgt ataacttagg acgaccttta tagacgccgt ttgctttagc
     2401 gattttaatt ccttgtgcct ggcgtcgttt cgattcagta cgttcttgtt cagcaatcat
     2461 ggctgagatt taaatgatta agtctttgat gaatttatct aggagaggat ttccaattgc
     2521 ttcagccatg atcggtaaac tggtgataat aagtcgcaca ttcttcttct ttagataatt
     2581 cactgaatcg ataatctcgt cataattgcg tccaagtcga tcaatggctt ctacaataaa
     2641 aatatcttgc tctctgacaa aatctaaagc ttcctggaaa atcggccgat gactaatcgt
     2701 cgctccagat tgtttttcga taaaaatttt ttcagcgcca aactttttca tttcttcaat
     2761 ttgacgttgt tcattttgat cgatggatga aactctaata taagcaattt tcaaatttat
     2821 tccctacttt tctataacgt ttataataca ccctaatcat acgttagaac tctaaatttt
     2881 tcaatgttat atttttgtct atttagggta aactctaaat agacaaacgg tttttgaatt
     2941 ctaaaagtta aatagtttat aatagaagaa aaagaatggg gtgacgattt cttagattga
     3001 aaaggaaaga taaatattat agattattac ttttgacagc tgaagcagga tggatggatt
     3061 ttccagaagt tgtaaaagaa gttcaagaga taggtgcaac tatttctgat agccgctcct
     3121 ctcctatgaa ctatcaggat gcccatggaa ttaaaataat tgatcaagag agaaatgtca
     3181 gaaaatacac aacaataaat gagtgtgtaa ttaatgaaaa tctttgtagg gcaccgataa
     3241 cgaaacacat taagaataga agcaaagcaa aagacggaag aacttttact accttctaag
     3301 tttttttcat atttgtttgc tatacttaga tctaaggaat tagactttcg ctgcccatta
     3361 tttaagtttg aggtagcgtc ttgcgaggga tcaataatgg aagaagaaaa aatatttgaa
     3421 aaacgctggg aattggcttc agttgaacaa agagcaaggt accataatct aatgtcctct
     3481 taccgtaata ttgattggac ttacaaagaa aagaagtact tgctttggtt atgtcagtta
     3541 gatgtaaata cctttgaaac atttgaagtg attctagata agattaaaaa cagcaatgaa
     3601 aaaagagctg atctataatg gttcgctctt tttattttat taactctttg gttactatat
     3661 gtaaaataca gcttcatatt acaataaatt tcaggataca aatttattta agcatatata
     3721 aatataattg aaatgggaca taaatatatg agtatgagaa aggtgattcc atgtttaata
     3781 aatgggcaaa ccacccaatg aactcgagag gtagagataa tttgaaggag acggacaagt
     3841 tctgggaaga agtatatagt aaaaacggtt ctgttgcaaa gttttccaaa aaatctattt
     3901 tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa tgaatcattt taaaggcaaa
     3961 caattcaaaa aagacgtcat tattgtcgct gttggttact acctgcgtta caatctaagc
     4021 tatcgtgaag ttcaggaatt gttatatgat cgtggaataa atgtttgtca tactacgatt
     4081 tatcgttggg tataagagta cagcaaagtc ctctattatc tttggaagaa gaaaaataga
     4141 caatccttct attcatggaa aatggacgaa acctatatca aaattaaggg acgttggcat
     4201 tatctttatc gtgcaattga tgcggacggc ttaaccttag atatctggtt acgaaagaaa
     4261 cgggatacgc aagcagccta tgctttctta aaacgactcc ataaacagtt tggtgagccg
     4321 aaagcaattg tgaccgataa agcaccttct cttggctccg cctttagaaa gttacagagt
     4381 gtgggtttat atactaagac agagcaccga actgtgaagt atcttaacaa tttaatagaa
     4441 caagaccatc gacctattaa acgacgaaat aaattttatc aaagtctccg tacagcctct
     4501 tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg aagaaatgga
     4561 acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggaat aacagcctaa
     4621 gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag aaccgagaac
     4681 cttgggtttt caagtgcttt ttggttcatt aatcttaacc atagtgtttt taaagtggaa
     4741 aacaagcaac aaaaaaagta tgtggagcct tgtaggaata gttcttatta tttttactgt
     4801 gactgcagca acagaagcaa attattcgca taaaattcag tcgtataaag gtcgtttagt
     4861 tatgaccaaa aaagatgtta cacgttatac atcctcaaga actggaggcc tatatgacta
     4921 tgcaccatta gatgcattga aatataaaga tcatttaaag aaacatgaag taaaagttgg
     4981 tgatcggtgg gagaatgcat ctcataaagc atataattcc aaaatagtct atacagtaga
     5041 agatgccgat gagcaaaata tcacattacc tgtattcaat tattggggaa cagttgtgaa
     5101 agtaaatggg aaaaagagca ctatagaaaa tgacaatggt ttagtgtcta ttaaaggtac
     5161 ggggaaaaga actatagttg aggtttcatc tacgtatcca actacgatga ttctatcttt
     5221 attaattagt tcaataactt ttatattctt atttacacgt tatttaataa gatgctgcgg
     5281 tgaaatgaaa atagattaga tagatattat aaatatgttg attctatttt catagcaatg
     5341 gagaaaatac attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg
     5401 aaaagtgaaa agagcaaata taaaatttaa tgaaaatata gaactaaata ggaaaacgta
     5461 gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa attatataaa
     5521 ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata atttttaggt
     5581 ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc aactctttcg
     5641 tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac gatactagta
     5701 tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata gcattcctaa
     5761 aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac ttgaacatga
     5821 aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg cgcaacattt
     5881 attgattgca aaattatgtg acaaaaaaaa ctagtagaag aaagaataat ggcttgataa
     5941 taaagtacta cctattaaag gtagtacttt atttaattta gtataaatta acatactact
     6001 atattttagg tttcatatta tataaattaa taatatacta ccatttgttg gtagtataat
     6061 ttaaaggtaa taagttattc ttttattact atgtttgggt tttatagcaa agaaagttta
     6121 ataagagttt ggacctagca aacaatatta atgaggtcat taaatcttat tatctttagt
     6181 gatataacga aatatctaca cagacaaaat gatagcgtaa aatattttgt gtaaatagat
     6241 atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt
     6301 ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat
     6361 aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg
     6421 caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca
     6481 ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat
     6541 ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa
     6601 agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg
     6661 tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt
     6721 tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa
     6781 tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt
     6841 tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt
     6901 gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta
     6961 aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac
     7021 gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct
     7081 agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttcaaa
     7141 ttagtttatc aagcaagttc aaaagaagaa gcaaataatc aaataaggtt tatgatagat
     7201 aaatggaaaa agcagtatcc acgagtggtt aaattgctca tgaatcctgc tatattaacc
     7261 ttttataatt tccctccgtc aatcagaaga actatttatt caactaattt gattgaaggt
     7321 tttaacaaac aattaaagaa atataccaaa agaaaagagc aatttcctaa tgaagaatct
     7381 ctggagagat ttctagtatc tcaattcaac aactataatc aaaagtttct atgccgagtt
     7441 cataaaggtt ttaaagaaat acatgataca ttagaatcaa tgttttaact tgatcaacag
     7501 aatgggcttt tccatttaca caaaattatt gacgctcccg acaaaattat gatcttgcgt
     7561 aaagttataa aatattaata aaaatatttt attaatgaaa agcttggtag cacggttatt
     7621 catatgctaa tttatttcac tttatagtaa ggggagttct tagtatattg ttcaaactta
     7681 aatttcatat aatttaatta tgtaaattag ctattctaag aacttctacg gataactgcc
     7741 cgtctaaaat ttaaataaga gcttagaatc ttcatataag taaggttaat ttttttgtac
     7801 aaaaaagtcg gaatattctg agttactgaa atgatataaa atcataaact aaaccccttg
     7861 attttcttca cttttaacag agtatatata taagttcatg attatggtaa aatatatata
     7921 taatcatgaa ctcagaatat ttttaagaag ggaaacaata gtgagctata atgtacaacc
     7981 gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa caagtaatcc
     8041 agaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta tgtcagatat
     8101 tataggatta aaagttggag acatacgagg taaaagtaag cctattattg ttgaacataa
     8161 aacaggtaag cgaaaaccga tttttataga taatttgaga gaagagattt tattatatac
     8221 tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg caacaaggtc gacatataac
     8281 aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggaa gagatgatat
     8341 aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa ctagagacat
     8401 tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa ggtacattgg
     8461 tataactgaa gatgaaatag gggcatcgtt acgtggtttt aaattgggag gctaattgag
     8521 atattaggat atattttttg tgtataatga aaacttagaa ggtgagatta atgaagataa
     8581 ctgaattttc ggaagtattt aaaacgttta gtaacattta caatagaata ccagagatag
     8641 aacaaaatgt tgtagttgat aatgattatt atatagagaa tgattttaat aaaccaagaa
     8701 aatatgacaa cctatacaat tttatacaca atagaacaac aataaataac aacagtaaca
     8761 ctcgagtttt gattaattca gcatattttc atattaaata tttaaagaaa tatgagtatg
     8821 aagaagttgt attagattat gggctaaata gacagtcatt tttaatttat gcaataaata
     8881 acggaaattt gccaagcaat aaaaagaaca taattattga aacatatgcc tatctggagg
     8941 ggggatctaa aaaactaatt aagtcatgta atatagagta tcaattatca gtaggagaaa
     9001 ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa aaaaattttt gccagcattc
     9061 atgattatat aggtttga
//
LOCUS       DACCQP010000097         9078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCQP010000097 DACCQP010000000
VERSION     DACCQP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.100"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(53..316)
                     /locus_tag="IVO87_002503"
     CDS             complement(53..316)
                     /locus_tag="IVO87_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7450655.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(415..1710)
                     /locus_tag="IVO87_002504"
     CDS             complement(415..1710)
                     /locus_tag="IVO87_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7450656.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(1707..2978)
                     /locus_tag="IVO87_002505"
     CDS             complement(1707..2978)
                     /locus_tag="IVO87_002505"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7450657.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIEDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTIGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(3143..4012)
                     /locus_tag="IVO87_002506"
     CDS             complement(3143..4012)
                     /locus_tag="IVO87_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7450658.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(4304..5914)
                     /locus_tag="IVO87_002507"
     CDS             complement(4304..5914)
                     /locus_tag="IVO87_002507"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7450659.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(7107..8447)
                     /gene="glnA"
                     /locus_tag="IVO87_002508"
     CDS             complement(7107..8447)
                     /gene="glnA"
                     /locus_tag="IVO87_002508"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7450660.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(8475..8867)
                     /locus_tag="IVO87_002509"
     CDS             complement(8475..8867)
                     /locus_tag="IVO87_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7450661.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         2544 a         1964 c         1557 g         3013 t
ORIGIN      
        1 cagactatcc ctatcagata gtctgctaaa cgttgagtta tcagcgtttt tattattccg
       61 cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg gtccacacgt ccgtctgctt
      121 gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc agaagttact tcgacacgga
      181 tgactgggta tgtattaccg tcttcccatt caactgtttc ttgtgaagtt tttgttgaac
      241 ctgacaagaa tttgaaacca gttgttgaat ccatgaatac tactgggtgg taatctggat
      301 gaatgttttc tttcatgatt ttttcgctcc tttgccctgc tccatttgct gaaccagagt
      361 tgatttgtcg atttacaaca ttaacatctt aacatgtttc agtcaaggtt gcaattatct
      421 tttcagattt ctggtcggat tttttttacc aaaggagaca tcttgaaatt ctttgaaaaa
      481 ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt tgttccgtat attctaatga
      541 atctccagtc atattttttc gaagtttcca aatttcttct aattcttcac ttggcatcaa
      601 taattcttct tttcgggtac ctgatttttt gatatcgatg gctgggaaga tccgacgttc
      661 agataattca cgtgaaagtt gtaattctag atttccagtt cctttgaatt cttcatagat
      721 cacgtcatcc atacggcttc ctgtatcgac taaagcggtc gctaaaatcg tcaaactgcc
      781 accttcttcg atgtttcttg ctgccccgaa aaatttctta ggtttataca atgcggctgg
      841 gtcaatcccg ccactcaatg tacgtccgct tggtggaacg actaagttat aggcccgagc
      901 aagtctagta atactatcca tcaagataac gacatctcgt ttatcttcga ctaaacgcat
      961 tgcgcgttcc aatactaatt cagaaactcg agtatggttt tgcggctgca gatcaaatgt
     1021 agaagatacc acgtcgcctt tcacgcttcg ttctagatcc gttacttctt ccggtcgttc
     1081 atcaatcagt agaacgatta attctacttc cggatagttt tcggtgatac catttgcgat
     1141 ttcttttaat acagaagttt tccctgcttt tggcggtgcg acgatcaatc cacgctgacc
     1201 aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat agtctttgcg gtgttgtttc
     1261 cagttgaagc tgtctttgcg gataaagagg tgttaaggct ggaaaatgcg gacgttcttt
     1321 tgcttcttcc ggatcttttc cattaacgct ttccacgtgc atcaaaccat aataacgttc
     1381 cgattcttta ggtggacggg cttttcctgc cactttgtcc ccatttctca aactgaatcg
     1441 tctgatttgt gaggcagaaa tatagatatc ttctgcactt ggtccataat tgattggccg
     1501 taaaaaaccg taaccatctt gaccaacgat atccaatatt ccttccatat aataaaatcc
     1561 ctgtttctct gcttgtgcac gaatcacagc tagagataat tcctttttat tcatcttact
     1621 gtaataagga attttaaatt ctttggcata actataaact tcttttaacg ttttgttctc
     1681 tagttctgcc attgtcaaat aatcgctcat tcagctccca cttcctctat catttcaatt
     1741 gatgccccta aggctgttag tttctcgata atatgatcat aaccgcgtaa gatataatct
     1801 acattgtaaa tcgtcgttgt cccttctgcc aatagtccag caatcactaa acaagcacct
     1861 gcccgaagat cagaagccac gacttctgtg ccgtgaagtt tatttggacc attcacgatg
     1921 atcatatttc cttccacggt aatatcagcg cccattcgtg ctagttccgg cacatggttc
     1981 acacgtttcg catagatcgt atcgatgatt tctgaagtgc ctatagtcat caatagtaac
     2041 gcagtaagtg gctgctgcag atctgtggca aatcctggat aaggataggt catgatgttt
     2101 gcgggtttta attctttcga ggggtaaact tcgatttcat cttcgctgat tttcatatgg
     2161 acaccaattt cctgcaattt tgctataaag ctttccaaat gttcaaagat cacatttttc
     2221 actttgatac catttcctac tgcagctgct aatgctagat aggttcctgc ttcgatgcgg
     2281 tcaggaatca tgaaatgccg acagccatgt aatgtttcga ctccttcgat acggatcaca
     2341 tctgtacccg ctcctcgaac ttttgccccc atattattca gtaaagttgc cacatctatg
     2401 atttccggtt ctcgtgcagc gttttcaatt actgtctgtc cttttgcttt tacagcagct
     2461 aacatcacat tgatcgttgc tccgactgaa accacgtcca tgaaaatccg attgccacgc
     2521 aatcctttat tttctgtccg taaatacatg gcaccatgtt catttgtcac ttcagcacct
     2581 agagcttcaa atcctttgac gtgtaggtca atcggtctag ggcctaaata acagccacct
     2641 ggcaggccta ctaccgcttc gccaaattta ccaagcagtg ttcccataaa ataataagaa
     2701 gcacgcaaac tattgatttt tccgtgcggc atcggaatcg atacgacttc tcgaggatcg
     2761 atttctagta tgttgttgga aaaatggaca gttgcgccca tgatctctaa aatttcaatt
     2821 aatgaatgaa catcttctat gtccggcaca ccttccaaga tcactgggga atcagctaag
     2881 attgcagctg gaatcaatgc gactgcacta ttttttgcac cgctgatcgt tacttcacca
     2941 gctaaaggtc ggtttccttt gattacgatt tttttcatct gtttccacct tgtttattaa
     3001 tcttttttca tggatatgct tgtattttat catagataaa aaaaagaaac gagcatggtc
     3061 tgccgtttta agtaaaaaag aatggagagc gacttcaaaa gtcgttctcc attcctatat
     3121 atcgtatctg ttatgtggat gcttacgctt tgttagctga accgaaccag tcgatatgag
     3181 atttagcatc ttcaacgatt gctgctttac ctggagctaa taatttacga gggtcgaatc
     3241 ctttaccttc tttatcttta ccagcttcga tatatttacg agtagctgca gcaaatgata
     3301 attggaattc agtgttaaca tttacttttg agatacccat tgagatagct ttttcgattt
     3361 gatcttgagg aatacctgat ccgccatgta atactaaagg catgtctgga cctacagcat
     3421 cagcaattgc ttgtaagtgg tcaaaagcta atcctttcca gttttctgga tattgaccat
     3481 ggatgttacc gataccgcaa gctaagtagt cgatacctgt agcaaccatt tgtttgcatt
     3541 cttcaatatc agctaattcg ccgttaccga tgattccgtc ttcttctccg ccgattgaac
     3601 caacttcaca ttcaactgag ataccttttg catgtgcttt ttcaacaacg tctttagcta
     3661 atttcaagtt ttcttcaaat ggtagatgtg aaccatcgaa cattactgaa gtatagccta
     3721 attcgataca ttccaaagca gcttcgtaat caccatggtc taagtggata gcaactggaa
     3781 cagtgatgtt cattgcttca actaaatcag tgattaaatc tttacagatt ttgtaaccac
     3841 ccatgtattt agctgcaccc attgatgttt ggataagtac tggtgcttgt tttgcttcag
     3901 ccgcttcaag gattgcttga gtccattcca agttgtttgt gttgaatccg ccgacagcgt
     3961 aaccgccttt acgagcagct tttaaaaatt cagctcctga tactactggc ataataaaat
     4021 tcctcctaag ttttgtaaaa ctaaatttgt taagacaatt cttaaccaac gaccctattt
     4081 taccaaacat ttcggtagtt ttctactaaa atcaatgaaa atgaaaattt tcaagaagtt
     4141 ttttgcaaaa aataaaatat ccaatagcgc tttaatcgaa aaaagtcttt tgctttctat
     4201 cttttttcct acaaataata acaaatcaga aaaagagaaa agtgacaaga aaattgctta
     4261 tccccttctc ttgctacttt tctctttcta tcaagttcaa aaattatttt gtttcgtgat
     4321 ctgctaatga tgctccgaca aatgctttga ttaatttttg cgggcgattt ggacgagaaa
     4381 tcaattctgg gtggaattgg catcccacaa agaatttttt ctctggcagc tctacgattt
     4441 ctactaaacg gttatctggt gagacacctg aaaagactag cccttgttct tcaaacaatt
     4501 gacgatattt gttattgaac tcatagcggt gacggtgacg ttcttgcaca acatcggctc
     4561 catcatatgc ttcagccgtc ttcgttcctt ttttgatttt acatgggtat aatccaaggc
     4621 gtaaagttcc acccaaattc tcgatgtttt cctgatcagc cattaaatca ataatattgt
     4681 ttgctgtatc tggattcgtt tcagctgatg acgcatcttc taatccaacg acgttacgtg
     4741 caaattcgac acaagccatc tgcataccaa gacaaatacc taaaaacgga acgtcatttt
     4801 cgcgagcata acgaatcgct tcgattttcc cttctactcc gcgatcacca aatccaccag
     4861 gaaccaagat tccatctgcg tctttcaaac gttcttctac attttctctc gttaattctt
     4921 gtgctttgat ccaatcgatc tcgatgtctg aattataggc gaaaccagaa tgtttcaatg
     4981 cttcaactac tgagatatat gcatctggta actcgacata tttaccgaca agcgcaatcc
     5041 gtactttttt cttcaagttc aagacacgtt cttctaattc tttccattct gtcatgtctg
     5101 cttctggtgt atctagtttt aaatggtcgc aaacaatttg atccattcct tgtgcttgta
     5161 aaactaatgg gatggagtaa agtgtatcta catctggcga ctcgataact gcttctggtt
     5221 cgacatcaca aaattgtgcc aatttgtttt tcacgctttg agaaacaggt tgctctgtac
     5281 gtaccaccaa aatattcggt tggataccta aaccacgtag ctcttttaca ctgtgctgcg
     5341 ttggtttagt tttcatttct ccagcagctt tcaagtaagg aatcaatgtt gtatggatat
     5401 acataacatt atctgtaccg acatctgctt tcatttgacg aagtgcttca aggaatggca
     5461 aagattctat atcccctact gttcccccta cttctgtaat gatcacatct gaatcagtca
     5521 ttttagctgc tcgcatgatt ttttctttga tctcatttgt aatatgagga ataacttgaa
     5581 cagttgctcc caagtattcg cccttacgtt ctttacgcaa aacttcagaa tagatttttc
     5641 ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat aaaacgttcg tagtggccca
     5701 gatccaaatc tgtttcagca ccatcatctg ttacgaatac ttctccgtgc tgatatgggc
     5761 tcatcgtccc cggatccacg ttgatgtatg gatcaaattt ttggatagtt acttttaagc
     5821 cgcggttttt caataagcgt cctaaagaag ctgcaacaat cccttttccg attgatgaaa
     5881 cgacaccgcc ggtcacaaaa atatattttg tcataagttc aaaaactcct ttttaattta
     5941 tgtgcaggaa aagtctcaga caataaacaa agctccctat tcttcaataa gaatagggag
     6001 ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc caaggtgtat aatacaagct
     6061 gactaaggga aagtcaagta ctcaaatgaa tactatccgc ttacaatttt ttctttttcg
     6121 atatcctttg agaaattatt tcttaagtcc tttatgagtg caaaaaaaat ttaacccaca
     6181 taataagctt gatataaaca tagctaacgt taaacctaag caaatcagaa aatcgttact
     6241 gaaaaatttt aagtgaaata atagataaaa aaataaaaca aacattatga tagataatat
     6301 cccacctaac aaagaactac tttttcctac ttgcagcaac agtttatata gcttatcgta
     6361 gaaacaatct ttttctttca cccttattta cctcctttac gtaaaaaacg tagtccacag
     6421 ttctcggatt tttggcatat cttttctatt aaataatatc aaaaacaaaa caattccact
     6481 aattttttca aagatgacct ttttttcaac aaaagtgcct tgtagggatt cattgaaggt
     6541 aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc cttatttcta
     6601 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca tcaaacatga
     6661 gaagctgttt ttgaaattat ttcttgtgat tcggagttaa atggtaggct cacaacctct
     6721 ggataaacgg tattcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     6781 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacgtcttt ttcaaaacct
     6841 cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agttcagcca attcaaaaac
     6901 aagaaaagct gtttttgctt ggctattctt attacttgtt gcagggcagc tttttcacaa
     6961 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     7021 atatgaggaa ctatttttcc gttagtttct ctaatactca ggtcaaaacg ctgatttcac
     7081 agcctctaat tcacggtatt cctgttttaa taaagttcta gatattgttc tctttcccac
     7141 tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt ttgcttcaac aaaatttgag
     7201 aagatatgtt gtcctaatgc gtcaatcatt acattatcct tacgcaattc tttgatagca
     7261 ttatggatag ttgaaggtaa gtcctggata tgtgcagctt ctctttcttc ctcatccatt
     7321 acataaatgt tacggtcaac agctgctggc ggtgtgatct gattacggat accgtctagt
     7381 cctgcttgca acaatactgc cattgccaag tatgggtttg cagatggatc aactgaccgc
     7441 aattccaaac gtgtggataa accgcgagat tctggtacac gtactaatgg cgaacgatta
     7501 cggccactcc atgcgacata aacaggcgct tcataacctg gaacgagacg tttataagaa
     7561 ttgactgttg gattgcaaac ggctgtgtag gcacgagcat gtttcagcaa accacctaag
     7621 aaatgataag ctgtttcact tagttccaat tcaccgtctt tatcatagaa aacatttcct
     7681 tcatcattga acaaagacat attgcaatgc ataccagaac cattgatccc aaataacggt
     7741 ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa tcgttttgac aacgagcttg
     7801 aaggtttgga tattatcaca agcatctacc acatctgcat atttaaagtc aatttcatgc
     7861 tgtcctggcg ctacttcatg atgtgaagct tccacttcaa agcctagact ttctaattcc
     7921 aacacgatgt cacggcggca attttcacct aaatctgttg gtgcaaaatc aaaataacca
     7981 cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt ctaatttaaa taagaagaat
     8041 tctggttcag gtcctaaatt gaaagaggta aaaccaaggt cgcgcatatc tttcaatgct
     8101 cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc catccggatt atagatatca
     8161 caaattaaac gagcaacttt tccatgagca ctttcccacg ggaagatcat ccaagtggag
     8221 agatctggat acaaatacat atcgctttct tcaattcgaa caaatccttc aatcgaagaa
     8281 ccgtcaaaca tcattttatt ttctaatact ttgtcgatct ggctcactgg aacttctaca
     8341 tttttgatcg tacctaagat gtctgtgaac atcaatcgta aaaatcgtac gttttcttcc
     8401 tcaatgattc gtttgatttc ttcacctgtt atctgtttct ttaccatggt atccctcttc
     8461 cttttttaat ttattcagtc aaagaattgt ctttgcaatc cttgcgactg ataatgacct
     8521 gttttgctta acccaccttg cgccaaaatc tcatcacgaa aaatttgtcg aacgtcatga
     8581 tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg cctgtgccat ttcgtataca
     8641 cgtttgatcc cagccatatt caatccctct gaaagatagt ctttaatctc aagcaacata
     8701 tcaatatcat tcagcgaata catacgtcgg tttccttcgc ttcgttccgg atgaactaaa
     8761 tcttgttctt cataatagcg aatttgccga gcagacaaat cagtcagctt cataactgta
     8821 ccaattggaa atacggacat agatcggcgc aattcctttt ctcccatcag acgtcccctt
     8881 tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa tattgtcaac tgcatatgtt
     8941 aggttttcta acatgagatc tctatttttt ataaaataac aaaaaaataa gaacacaaaa
     9001 aaggtatcta gttgattttc gtaccagata ccttttattt tttattatac tgtactatcc
     9061 tttattagtg tttttatt
//
LOCUS       DACCQP010000098         8892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCQP010000098 DACCQP010000000
VERSION     DACCQP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.101"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(360..857)
                     /locus_tag="IVO87_002510"
     CDS             complement(360..857)
                     /locus_tag="IVO87_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7450662.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(897..1706)
                     /locus_tag="IVO87_002511"
     CDS             complement(897..1706)
                     /locus_tag="IVO87_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7450663.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2040..2897
                     /locus_tag="IVO87_002512"
     CDS             2040..2897
                     /locus_tag="IVO87_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7450664.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2890..3792
                     /locus_tag="IVO87_002513"
     CDS             2890..3792
                     /locus_tag="IVO87_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7450665.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3825..4175
                     /locus_tag="IVO87_002514"
     CDS             3825..4175
                     /locus_tag="IVO87_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7450666.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4209..5147
                     /locus_tag="IVO87_002515"
     CDS             4209..5147
                     /locus_tag="IVO87_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7450667.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5225..6118
                     /locus_tag="IVO87_002516"
     CDS             5225..6118
                     /locus_tag="IVO87_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7450668.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6156..6719
                     /gene="amaP"
                     /locus_tag="IVO87_002517"
     CDS             6156..6719
                     /gene="amaP"
                     /locus_tag="IVO87_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7450669.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6733..6921
                     /locus_tag="IVO87_002518"
     CDS             6733..6921
                     /locus_tag="IVO87_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7450670.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6934..7836
                     /locus_tag="IVO87_002519"
     CDS             6934..7836
                     /locus_tag="IVO87_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7450671.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQERVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7868..8110
                     /locus_tag="IVO87_002520"
     CDS             7868..8110
                     /locus_tag="IVO87_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7450672.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8366..8737)
                     /locus_tag="IVO87_002521"
     CDS             complement(8366..8737)
                     /locus_tag="IVO87_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450673.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3101 a         1535 c         1719 g         2537 t
ORIGIN      
        1 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac ttggctgttc
       61 ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt
      121 tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
      181 aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc
      241 gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa
      301 aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt
      361 tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt
      421 tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc
      481 aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct
      541 ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga
      601 atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc
      661 aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg
      721 aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc
      781 aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata
      841 tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa
      901 tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt
      961 gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc
     1021 aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca
     1081 aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct
     1141 aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc
     1201 ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga
     1261 tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa
     1321 taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct
     1381 cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta
     1441 tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca
     1501 atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct
     1561 ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat
     1621 ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg
     1681 tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt
     1741 tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg ctttgtcatt
     1801 atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta gtttttggtc
     1861 aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt
     1921 gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa
     1981 tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga
     2041 tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt
     2101 acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat
     2161 tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa
     2221 ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag
     2281 acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact
     2341 tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag
     2401 acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg
     2461 atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca
     2521 attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa
     2581 ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga
     2641 atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc
     2701 tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa
     2761 atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag
     2821 atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag
     2881 gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta
     2941 ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt
     3001 cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca
     3061 gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt
     3121 acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg
     3181 gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct
     3241 ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt
     3301 gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt
     3361 tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg
     3421 caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat
     3481 gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa
     3541 ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa
     3601 gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag
     3661 gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt
     3721 tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta
     3781 gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt
     3841 ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg
     3901 cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct
     3961 tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc
     4021 cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca
     4081 tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt
     4141 ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga
     4201 ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa
     4261 aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg
     4321 aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc
     4381 ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc
     4441 tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg
     4501 ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa
     4561 agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt
     4621 acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag
     4681 cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca
     4741 tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt
     4801 ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac
     4861 agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc
     4921 tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg
     4981 cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat
     5041 taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag
     5101 cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa
     5161 aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac
     5221 agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc
     5281 taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg
     5341 agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg
     5401 agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc
     5461 tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt
     5521 tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga
     5581 aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg
     5641 acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt
     5701 tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc
     5761 aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt
     5821 agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct
     5881 tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca
     5941 actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga
     6001 acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc
     6061 aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga
     6121 agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg
     6181 gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa
     6241 cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg
     6301 tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac
     6361 ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg
     6421 agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg
     6481 aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg
     6541 taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag
     6601 aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga
     6661 aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg
     6721 gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta
     6781 cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc
     6841 ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta
     6901 gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac
     6961 aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa
     7021 tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca
     7081 gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca
     7141 cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct
     7201 tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt
     7261 agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga
     7321 aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt
     7381 gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt
     7441 ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc
     7501 gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc
     7561 aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat
     7621 atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa
     7681 gaatttgaaa aagatcaaga aagggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc
     7741 ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa
     7801 aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga
     7861 acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg
     7921 agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt
     7981 tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc
     8041 aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg
     8101 aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata
     8161 agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat attatcgaag
     8221 atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac ttttgtcaca
     8281 gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc cttaaatgtg
     8341 tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat tttcttgaga
     8401 taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg atccacaaat
     8461 ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag agattgaaaa
     8521 agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa taatagctcg
     8581 taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca gcaatcgtat
     8641 catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga atatagatca
     8701 cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta attgtgtgag
     8761 ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat
     8821 gttatctaat gtatttttaa ataaataatc attattatca aaataatgtc agactgtaga
     8881 caaagtccat tt
//
LOCUS       DACCQP010000099         8490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCQP010000099 DACCQP010000000
VERSION     DACCQP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.103"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IVO87_002522"
     CDS             complement(<1..173)
                     /locus_tag="IVO87_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7450674.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVO87_002523"
     CDS             complement(593..1714)
                     /locus_tag="IVO87_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP7450675.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVO87_002524"
     CDS             2042..3451
                     /locus_tag="IVO87_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7450676.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVO87_002525"
     CDS             3448..4176
                     /locus_tag="IVO87_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450677.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVO87_002526"
     CDS             complement(4304..5215)
                     /locus_tag="IVO87_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450678.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVO87_002527"
     CDS             complement(5232..6239)
                     /locus_tag="IVO87_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7450679.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVO87_002528"
     CDS             complement(6236..8365)
                     /locus_tag="IVO87_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7450680.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVO87_002529"
     CDS             complement(8370..8477)
                     /locus_tag="IVO87_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450681.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACCQP010000100         8357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCQP010000100 DACCQP010000000
VERSION     DACCQP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.104"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(65..370)
                     /locus_tag="IVO87_002530"
     CDS             complement(65..370)
                     /locus_tag="IVO87_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450682.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(367..528)
                     /locus_tag="IVO87_002531"
     CDS             complement(367..528)
                     /locus_tag="IVO87_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7450683.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(543..902)
                     /locus_tag="IVO87_002532"
     CDS             complement(543..902)
                     /locus_tag="IVO87_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7450684.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(899..1750)
                     /locus_tag="IVO87_002533"
     CDS             complement(899..1750)
                     /locus_tag="IVO87_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450685.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1767..2597)
                     /locus_tag="IVO87_002534"
     CDS             complement(1767..2597)
                     /locus_tag="IVO87_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7450686.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2600..3286)
                     /locus_tag="IVO87_002535"
     CDS             complement(2600..3286)
                     /locus_tag="IVO87_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450687.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3292..3531)
                     /locus_tag="IVO87_002536"
     CDS             complement(3292..3531)
                     /locus_tag="IVO87_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450688.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3857..4045)
                     /locus_tag="IVO87_002537"
     CDS             complement(3857..4045)
                     /locus_tag="IVO87_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450689.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(4066..4350)
                     /locus_tag="IVO87_002538"
     CDS             complement(4066..4350)
                     /locus_tag="IVO87_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450690.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(4352..4546)
                     /locus_tag="IVO87_002539"
     CDS             complement(4352..4546)
                     /locus_tag="IVO87_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450691.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(4543..5319)
                     /locus_tag="IVO87_002540"
     CDS             complement(4543..5319)
                     /locus_tag="IVO87_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7450692.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(5446..5667)
                     /locus_tag="IVO87_002541"
     CDS             complement(5446..5667)
                     /locus_tag="IVO87_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450693.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            5968..6312
                     /locus_tag="IVO87_002542"
     CDS             5968..6312
                     /locus_tag="IVO87_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7450694.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            6348..6743
                     /locus_tag="IVO87_002543"
     CDS             6348..6743
                     /locus_tag="IVO87_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7450695.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            6796..7107
                     /locus_tag="IVO87_002544"
     CDS             6796..7107
                     /locus_tag="IVO87_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450696.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            7119..8267
                     /locus_tag="IVO87_002545"
     CDS             7119..8267
                     /locus_tag="IVO87_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7450697.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         2518 a         1568 c         1327 g         2944 t
ORIGIN      
        1 tctttctgaa tcttcatcat gattttcttg aggatgatgt cactgtattg tgtaatgaga
       61 tccattatct ctcctccaca tacctaaact gtcgtccttt tgaatcaatc cataagctcc
      121 tagctctatc ccagataatg tttttgctta atccagtaat ttcagataac tgttcagctg
      181 tacctgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc gttttccgtt
      241 taggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact atttctttgt
      301 cttcctgcca agattctgac ttggttaatt cagcaattcg tttcattgtc gctttcttat
      361 ccacgctcat tcctccaatc tacgaatttc ccttcttaag ttctctatgt gcaaatcgat
      421 tgcctttctc gccgtttcat tgaccatcac tgcctttgtt cgttctagat cgtcaatttc
      481 acgctgaatg tttcgaatac gcatttgaat cacttcttct gttgtcatga tggaccacct
      541 cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat caacggcgct
      601 tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg cattcctttg
      661 acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg aatatactcg
      721 atcttgcgat acttctttcc gtctagttca aatttcggca tcagctcaaa tctttcctga
      781 agttttactt tccagccatt tgcttcagct tgccacaagg ctagatcgta atactctgct
      841 tctgcgatag aatcaaactt gatacctcga tgaacagttt ttttattacg gtatttattc
      901 atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg aatttcagcg
      961 cataaccttc tgaattctta aatatcctag aaacaattct ttcaccgtag gcttctctta
     1021 gttcaggacc agataagttt gttgtgatga tcgttgcctt gttctgtctg gcttctaaga
     1081 gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccacta cctaattcag
     1141 ctccaatatc gtcaaaaacc accaaatcag ttgttttaat atcggctata agcgatcctt
     1201 caatttcttt tctcagttca gtattgttat aagaaaactt tatttgctct aataattctt
     1261 gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt tcccaagctg
     1321 tggccattga taagtggctt tttccgcttc ctgatttccc tgatagaatg aaatgtgcag
     1381 gatggttcag cagaacctca ttcgtatagc tttttgcttt ttctaaagcg atttttgttt
     1441 cttgatccac tacgtgataa ttttccattt tgcatttaaa caaagtttta tctgttaata
     1501 ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctatcgtta tatatccgtt
     1561 cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg catgttggtt
     1621 tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt tcgctacatt
     1681 ctggacattt tccgctttgc actaatactc ttcttattag tttctccata gcatttgcta
     1741 ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc actaggattg
     1801 ctgtactgta gtttttgact ttgcttttta tgattcttct tgtctgcttt gatttcgaat
     1861 ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc aaaccaaaat
     1921 tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagatgc tttatctctt
     1981 tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt catttctttt
     2041 ggaaaatctt cagttaaatt actttgcaac tttttagcaa ggcgtaagtg ttcgtcagaa
     2101 tacttacctt tcttttcttc tttatctata tctttatctt cttctatatc tttatctgta
     2161 ccgtcacgtg acgtcacgct aacgtcattt tccaatttga gacgttcctg tctctttctt
     2221 tccctgtatt tacggtttct ttcagcattt tttagcctta ctttatccat accctcgata
     2281 ttttgatgtt tttcccaatt actgatggca attagtccat cactgcttag atcaatcatg
     2341 ttgaaatttg ccaatgtagt tagcgctaag cgaaccgtat ttacgttttt gccaaacaat
     2401 gtagcaagca tttcttcggt ataaggcatg ttcctctgga tatatatcag accatcgtcg
     2461 ttagtctttc ctgctaaaac tagtaatcga atccatataa cgatgatggc atccgactca
     2521 ggaacagctt ggattaatcg tattttttca tcgtcaaaca tagtagtttt aagtttgatc
     2581 caacttatct cagccaaatt tatcctccta tccttaactt tttaattgtt tcctggttta
     2641 acttgatccc tttgatttga tatttatttt tgaaattgat cacacctatt ttgtgtttct
     2701 ctgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgaatgatca acttctttgc
     2761 gctttcgtct tcccagcgct ttgtcaaagt gatcgatatc agctcctgtt ttgccacaga
     2821 tgcagcagac tctttttgta atgcatttgt agaagtaata ctcttgattc gctggtaaaa
     2881 tctcatatcc ttctttgaaa ggaatatgat gttcaaagat gaaatctagg atgatattcg
     2941 ctaagatatt ggcatcactc acggttgtat tcgattcgtc tttgaggctt atttcgcgcc
     3001 ctgtgacacc ttcaaaacgg aagtagaaga attccttcca gaagtccgtt ggcatgcctg
     3061 tatcgatgaa aatatcgcct atgagcgcat agatgaagtt tctttgctgt actgtgaacc
     3121 gtcgtggatc aataaagcga atttcaatga ctcgatcgcc atcatatccg tcatacatcg
     3181 ttttcagccg ttcgatattt acttcttcgt tgattgttgc accaatgtcg ttacctttaa
     3241 atttctttag taccgccgag tatgaatcta ttagtggttt aaacactcat atcacttctc
     3301 ttttgtttct tctctgtact gatcttcaag ccaattaacg cctcgtttta gaatgcccaa
     3361 gtctctcttg gtccatttac tgtcatcagc ggttatggaa gctgcatcag tcaatgcaac
     3421 aattgcttca tcaattgatt tttcgtactt gttagcaacc agttgtaaat tcgatttgcc
     3481 ccaatcagga tcggccaaat attgatctag agtggaaagt tcattttcca tatgttaaaa
     3541 tctccttagt tatgattttt tgagtgactc attgctttgg tcggcggagt cactttttta
     3601 tttgttgcca tgcttttttc ttttcgatat gttgcttgct taaaataata gaacggctat
     3661 ttgcccacca attatcagca atcactttac cgatttttag cgcttcttct cgtgccatag
     3721 ttgctccttt cttttgagtc aagcagattg attaaaacca tcaatgctgc gaacaagctt
     3781 cctccgataa tactttggtg cgctacaatc actaataacc ccaaaatgaa tcctataaaa
     3841 agtgtgtctg tcttcttcat aatctaatct ccctattttt tatttctagc attctcaaat
     3901 cctcaagttc agaagcaatt agttcagctt gtctatctga tagctcatcg gattttctaa
     3961 gcgctgcacg atcatcttgt aattgttttc tacgctgttt aatcagacct agaatttgat
     4021 gttcttgttg caatgtgtag gacataaaat cattctcctt tgcctttaga actcaaagtt
     4081 ttctttcaaa aatctttgga gttccgatcg ttcaattctg atgtctaact tgctccactg
     4141 ctgtgttttt aagcctaggc ttatccaatg tgttaatttg tcatcaccaa tgcctaaaac
     4201 tttttttacc tctgatttgt ttggatatgg aggaagctct actgatttgt tcataaggtg
     4261 taatcgttcg tccaatgaat taagaacagc attcgtaatc tgtgtagtta attcggaaac
     4321 tactaaatta tctggaattg ttatttgcat gttatttctc cttctctaac tctgctagtg
     4381 ctgcctcaat tggcttgatt tgtttatctg gttttctacg cccattcata atgtccgaca
     4441 tatatggtct agaaattccg atagtatcag ctaaccagtt ctgagacttt ccgtattttg
     4501 ctaattgaat tctgactttc aaaataaatt cttgtgacat cgtcattcct cctctaataa
     4561 atcgatttct ggaatatatc cttctttttt cagcgactca taaatgaaca gacgcccttt
     4621 ttgagtccat tttgtattca tcacaacttt tgttccgcca tcagatttcg gaatctcagt
     4681 tgtatgagat tttgtgtaac cctgtctcat gtgttttttg cataataacc attggttgcc
     4741 tacttttttc tgaataccta gcttatgaag tagtttgttc atctgttgtg gagacatccc
     4801 ataatcagcc gcgatttgac taatagttac tgaatctgta gaagataaga tactatccaa
     4861 atacgaaatt ttgggttcgt attctgcaat tttttgttct gcgattagtc ttccagtacg
     4921 ttcttctttc aacttagttg ctaattggat gattgtatct ggattaagca aagcttcttc
     4981 tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca cttcgctagt
     5041 cacccaacgt ttaaatttct tagcatttgg ttgtttgctt cctaaaatta aggagtacaa
     5101 acctgattcg ttaataatag ttaaccctct aggcgattca aaagtaccgt tttggtagtt
     5161 ttgccgatct tctacatcga cgtgtctgtt aatatctcta ctaccatttt ggtaccctag
     5221 aactttagcc acatcttttc caacaaaaaa tggttcatca tgtattgtta ctgttcttac
     5281 ttcatgttgt tcaaagttaa aaatttgcgg tgtgttcatt tttgctcatt cctttctttg
     5341 atataatttt gaatagaaag cgaggtgaaa atagtatgga agaatttaat atggatgtcg
     5401 acgccttatt caaacaaacc gtattcaaga ctgttaataa agaattcaaa atagacttcc
     5461 aaaatgacga atcttttcca acagaaattg aattattcga agaactttct cagagcgttt
     5521 ctgagtcttt ttctcgtcag ttgcaaaaga atttttttga tgctcttgtg gatgaatttc
     5581 atcaacaaca gcactaaact tttggtcttt aaaatttaaa gttattaaag ctccttttgc
     5641 ggaaggagtt tttttatttt gtttcatgat gtttcctcct tttctttaaa tatgtaagct
     5701 aataaaatta gctaattttt attgacattt ctatccaact gtcttatatt tatatacagt
     5761 taaataagcc tagaaaaacc ctaaaaatta acattcttag tttgccgacc tcgaattcgt
     5821 ttattttatt aggtgtattt cttgttgctt gttagcttat taaattagct tacgagttaa
     5881 atataagaca attggataga tttgtcaact acctttctat ctttttgtat aaattattat
     5941 cgtacgctct aggaaggttg atttgacatg cttttcgaaa gggttaaaga gctctgtaaa
     6001 aaaagaggta tttctatttc tgaattagaa aatagaatag gatttggaaa aaactctatt
     6061 tataaatgga agacacaaag tcctaaaata gaaactttgc aaaaagttgc tgactacttc
     6121 catgtatcaa cagattattt gctaggacgc acagatgatc ctaacgcggg agttgcacca
     6181 gaggaaagaa aactaaccgt ggaagaagct ttagcatctg ttatgagtag cgacggaaaa
     6241 ccgctcaccg ataatgatag ggaaatacta tcagctatga ttgaagcata tttagagaaa
     6301 aaagataact aaataagtag gtgagtcatt tggacagtca aattgaaatg ataattaatg
     6361 aactcggcgt taaggtagaa gagcgtgaaa accttgatgc cgatggccat tatgttgctt
     6421 gtatgaatac catagtaata aaagctaatt tatctaagta tagaagacaa agaaccttat
     6481 tacatgaatt aggacacgct tctaaacatc atgataatta ttttttatat aacttagcat
     6541 tctctctcca ttcaaaaatg gaatatgagg ctgatcgctt catgattgaa aaattattag
     6601 atagatatat tgcaaagtct gaattagaac cacacaatat caattacatg aaatttatag
     6661 aagataacaa tttaagcgtt cgcttcgaac cacttgtgaa agaattatta aaagctcgca
     6721 tctattgtta tgcagctctc taaaattttt taagcaaaaa agaacatacg ttcaaaaata
     6781 gaaaggtgaa caaaaatgat atatacagaa ttcaaagaat ggttagaaaa aagcacaacc
     6841 ggatacgaaa catttatcat caaagctact aattatcaaa ttgaaaaaaa caaaaataga
     6901 ccccaaaaaa aacgctggga tgataagaaa atagataaag ctgtattaga aatgtggaaa
     6961 caagtcgtga ctaacttgta tcaaacaatt cgtaaagaaa aaggagttcc attaattaac
     7021 gggaaggaaa tatggcttga atttatagag gaacaaggac tgatcgaatt tttcaatgat
     7081 agcatggcag aattagaatt tgaatagggg taatattgat ggcaatgata aaacaatata
     7141 aaaagaaaaa tggcgaaaaa gcatggtact ttaaaactta tctcggtatt gatccgctaa
     7201 ctggaaagaa aaaatatact actaaacgag gatttagaac acaaaaagaa gcaaaaacag
     7261 cactttctag gttagaacta gaattacaaa aaacaggaat gcccacaagt acaaatacta
     7321 ctttcaaaga agcagcagaa ttatggctag aaagctacaa aaaaactgta aaagaaagtt
     7381 catattcaag gactaaaata atctttaata aacatatata tcccaaattt ggaaatatta
     7441 agctttctaa aattaatacg gcatattgtc aaaaggtagt aaatgattgg agtgaaaaag
     7501 gaacttcaaa gcagtaccct cttttcataa actatatgaa taaagttttt aagtatgcta
     7561 taaatattgg tttaacatct gataatccaa cattaaattt acttattcca aagccacaaa
     7621 ttaaaacaga aaagaaatta aaattatata caaaagaaca gttggaatta tttctaaatg
     7681 aagtatctca agaacagaat ccatatttta aaaacagaga ctatacgctc tttagactat
     7741 tagcattcag cggatgtaga atcggcgaaa tattagcact cacttgggac aatattaatt
     7801 ttaaaacaaa tgaaatggcc attaaaaaaa ctgtagctcg ttcagataaa tattatatat
     7861 ctgaaactcc taaaaccaaa aaatcaaatc gaataattta tttagatgaa aaaactataa
     7921 agcaactaaa attttggaag ctcgaacaaa gaaagtactt atttcaatta ggatttacta
     7981 aagctaatta tttgtttacc aatgacgaaa ataatttcac aattaatcag tcagtggcag
     8041 aaagatacaa tatatatcgt gagcgtgccg gcttacctta tatcggtctg catggtttta
     8101 gacatacaca tgcatcaatg ctatatgagg caggcgcaga tcacaaagaa gtccaagaaa
     8161 gaatgggcca cgcaaatata aaaactacta tggacacata tacacacatt actaacagca
     8221 aaaaagaaga aacaacacaa aaactaacaa attatattaa cttctaagaa agtatggtca
     8281 aaagtatggt caaaaaaaat cccataattt acaaaaaagc tccatccctt gctacacaag
     8341 agatagagca gtttatt
//
LOCUS       DACCQP010000101         8320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCQP010000101 DACCQP010000000
VERSION     DACCQP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.105"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(31..2565)
                     /gene="secA"
                     /locus_tag="IVO87_002546"
     CDS             complement(31..2565)
                     /gene="secA"
                     /locus_tag="IVO87_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7450698.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(2787..3338)
                     /gene="raiA"
                     /locus_tag="IVO87_002547"
     CDS             complement(2787..3338)
                     /gene="raiA"
                     /locus_tag="IVO87_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7450699.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(3466..4185)
                     /locus_tag="IVO87_002548"
     CDS             complement(3466..4185)
                     /locus_tag="IVO87_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7450700.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(4182..5495)
                     /locus_tag="IVO87_002549"
     CDS             complement(4182..5495)
                     /locus_tag="IVO87_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7450701.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(5576..5896)
                     /locus_tag="IVO87_002550"
     CDS             complement(5576..5896)
                     /locus_tag="IVO87_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7450702.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(6004..6291)
                     /locus_tag="IVO87_002551"
     CDS             complement(6004..6291)
                     /locus_tag="IVO87_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450703.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6418..7062
                     /locus_tag="IVO87_002552"
     CDS             6418..7062
                     /locus_tag="IVO87_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7450704.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(7121..7828)
                     /locus_tag="IVO87_002553"
     CDS             complement(7121..7828)
                     /locus_tag="IVO87_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7450705.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         2283 a         1775 c         1452 g         2810 t
ORIGIN      
        1 aatttgtctc accaatcctg ctgtcagaaa ttagtttgca ttttttccat gacagttctt
       61 atattttttc ccgcttccac aaggacaagg atcatttctt ccgaccttgt tcacatggat
      121 tggctgtttt ttcgcgctag tattactatt ttctttggca tctccgtctt ctgagtgaga
      181 agctgttcct tgagaaacct gttcacgctg tacgttttga cggatctcag ctttcataaa
      241 gattcgagtg acttcatact cgatggcgcc taccatatct tcaaacatct tatagccttc
      301 tgtttgatat tcgactaatg gattgttctg tccataagca cgtaagccga ttgactgacg
      361 taattgatcc attgcatcaa tatggtctgt ccatttagag tccacaacac gcaagatcac
      421 gactttttca aattccaaca gttgctctgg accatttagt tgagccgctt tggtattaaa
      481 tacttcttgt gctcgttcgt ttagatactc tttgatctcc tgagatgttt ttccttcgat
      541 atctgcaaga gtaatactat cttcatggac aagagtactg ccggcaaaat cgatgatact
      601 ttccaaattc caattttctt tctctaattg tgtatgcgca tctactacac gactgattgt
      661 ccgtttgacc atgttcatta gtggcttgga taagcttttt tcttccatga ttacttcttg
      721 tcgctgttta tagattactt cacgttgttc acgcatcaca tcatcgtatt gtaagacgtt
      781 tttacgtgtg tcatagttat ttccttctac acgtttttgt gcagattcta cttgtttcgt
      841 cagcatcttg ctttggataa cagcatcttc ctcaccgatc ttcatccgat ctaagaaagc
      901 tttgattcgt tctgaaccga atcgtttcat caaatcatct tccaatgaca agtagaattg
      961 tgacatacca gggtctcctt gacgtcctgc acgtcctcga agctggttat cgatacgacg
     1021 agattcatgt cgttctgtcc cgataactgc tagaccacca agttctctta ccccaagtcc
     1081 taacttgata tctgttcccc gaccagccat gttggtagca atcgtcacag cacctttttg
     1141 accagcattc atgatgatct ctgcttcttt aaagtgattt ttcgcattca gcacttcatg
     1201 aggaatacgt tcttgattca gcatatctga taataactca gatgtttcaa ccgcaacagt
     1261 tccgactaac acaggttgac ctttgcggta gcgatctttg atatcttgca cgacagcgtg
     1321 gaacttgctt tgtaatgttg ggtaaagtaa atctggacga tcatcacgga taaccggacg
     1381 atttgtcgga atctggtata cttggatgtt ataaatttct ctgaattctt cttcttctgt
     1441 tttagctgta ccagtcattc ccgcaagttt cttgtacata cggaaatagt tctggaaagt
     1501 gatcgtcgcc atggttttcg tttcgtcttc gatctctacg ccttctttag cttcgattgc
     1561 ttggtgaagt ccatctgaat aacggcgtcc atccatgatc cgaccagtga actggtcaac
     1621 aatcagtact tttccgtctt ggacaacata atcgatatct aaaagcatga tatagttggc
     1681 tctcaatgct tgatccaagt gatgggtcaa tgcagtattt tcaatgtcgt ataagttatc
     1741 caaaccgaat gtttcttctg ctttttcgat ccctgcttcg gttaaactga ttgtttttga
     1801 ttgtacatcg attttataat cttcttcttc tttcaaacgt ttcacgaagt tgtctgctct
     1861 tgtatataaa gctgtggatt tttctgcttg ccctgaaatg atcagcggcg ttctcgcttc
     1921 atcaatcaag attgagtcaa cttcatcgac aatggcataa ttcagcggac gttgtaccat
     1981 ttgatggcgg taaacgacca tattgtctct caagtagtca aatcctaatt cattattcgt
     2041 actataagtg atatcacaat tataagcctc gcgtttttca tccgaagatt ttgagttgat
     2101 attcaacccc actgttaatc ctaagaaatt ataaagttcg cccatttccg tcgagtcacg
     2161 ggttgctaaa tattcattga cggtaactac gtgtacccct tcgccagtca gagcatttag
     2221 atatactggc atcgttgcgg tcaaggtttt accttcacca gtacgcattt cagggatatt
     2281 tccatcgtga agcacgattc cacccatcaa ttgtacgtgg tatggatata accccaatac
     2341 acgttttgct gcttctcgga cgactgcaaa agcttccgga agtaattgat ctaatgtttc
     2401 tcctttttgg tatctgccct tgaattcagc agtttttgcc tgtaattgtt catcagttaa
     2461 tgctgccatt tggtcagcgt atgcttcaac tttttttgca atgctatcta aacgttttag
     2521 ttctttctta tcgttttcga taagtgaacg aatcaaattg gccatgcgtt gtgtaatctc
     2581 cttttccttc tgtgtattat ccagactatg tagactttac ttttcatttt atcattagtt
     2641 atagataatt gaaatatctt aagcgtatta aagtaagaac ttcataattt attcatattt
     2701 catctaaaag aggaggaaac tcaaaaaaac ttgcggcagc tgtcagtagc tgctgcaagt
     2761 tttcgttgtt atttaagtaa ggctgatcag tctgtttcaa tcagtccgta ttttccatct
     2821 ttacggcggt aaacgatact tgtaccattt gtttctgcat cttcaaaaat aaagaagtta
     2881 tgtcccagca tgttcatttg taagactgct tcttcactat ccattggttt caatgaaagg
     2941 cgtttcgtac gtacgatatc taattcagaa tcattttcgt tttcgtcctc ttcattgaat
     3001 aaagcagcag catctgcagt attcatgccc gtttcacggg atttacgatt aatttttgtt
     3061 ttgaatttgc ggatttggcg ttccaattta tcaacaacca aatcgatact tgcgtataaa
     3121 tctggagaag tttcttcggc acgtaataca agataaggta atgggatcgt tacttccact
     3181 tttgctgttt tttctgtata aacttttaag ttcacatgcg ccgttgcatc gggtgcgtca
     3241 ctgaaatagc gttctagttt gcctactttt ttctcaacgt agtcgcgaat cgcttcagtt
     3301 acttcgatat tttcgccgcg tacattatat ctaaacataa aaattacccc tttcgtctac
     3361 cacaatcaag aaggagaagg accactcttt ccctcttctt aattctatta tatcgaactc
     3421 taggttattt gcaaattaaa tcgctatcaa atttccacat ctaattcatc tcgccaaaga
     3481 aatggttcgg atgattttcg gctgtgcgac ctgcaaacaa tctgctgcgt ggaaaagagt
     3541 ctgtcctgtt gtatagacat catcaataat caatattttt ttattttgta tcttagaaaa
     3601 ggtgactgca agttcaaaag gttgaggcat ctccaatctt tcctttcgcg tgttttttgc
     3661 ttgagccagc agatgcttct ctcttttgag tagcatctga aaaggaattc ttgctgcttt
     3721 caacatctcg cttacttgat tgaatcctct ttctgttcgc cttttttcag ataaaggtat
     3781 tggacaatag atgtatgtcg ggtattcttt ttttatctgc gtccatttat atgcaaaggt
     3841 gcctgctagt cggatatctc ccataaactt gtattgattg agccaggcct tcatattttc
     3901 atcgtacaca aaaaaggcac ggtgctgaaa atcataggtc ggatatttct tcttccattc
     3961 ttgacagtca ctgcagtaaa cgggaataga ctttactttc tttttcgtac tctctagatt
     4021 gaaatggatt ggtttttgac agccagaaca gcctcttgtt tccaataaat gaaattgtgc
     4081 gcgacaaact gaacaagtct gaggtaatct gtatttcgaa acaccaataa tctcgctgaa
     4141 tgttaaattt tggaagatat ctttactgca gcaactgcac ctcacagcat cttcttccct
     4201 tcttcattca tttctttgat ttcctgacaa gcttttcgaa tagctgcagt tttttcttca
     4261 tagaaaaagt atacttgact gttcgtataa ggccctttac gatcggctct tcctgcgatt
     4321 tgtaccaaag aagatttagt aaaaaccgga tgatccgctc ctaaaacgat aacggatatt
     4381 ctttcaaatg tgactccacg ttcaaggatc atcgtggtta acagtatatc atattttgct
     4441 tctcgcattt tctggacttt ttccgcccgc tgattgtctc ttgaagaaac atctgtgatc
     4501 tcatatctcg gaagagcctc ttgaagtgtg gctttcaaac gatgcatcat cgtgatactt
     4561 ggacaaaata aaaggatatg gttttcttta attaattgat gaatgatccg caccaaagat
     4621 tttatcttct tcttgttctt gcatttttgt cgccaattat tcatgaaaac caagacgggt
     4681 tcaggaagca aacgccgatg aaagcgtaaa gcaagtttgt ggatgtcaaa cgtctgcttg
     4741 atttccttta gtaacttttt atcaggtgtg gcacttaagt agatgaatcg tccgtctggt
     4801 ttaatcgctg tttgcacagc atgagctaat cctgcatccc cgttataagg aaacgcatcg
     4861 atctcatcga tcactattaa atcaaaggac cgataaaaac ggtatagctg atgaatcgta
     4921 cagataacaa aagaagtaaa ggtataagcc gttgctgtat tcccatggag caacgtgatt
     4981 ttttcttctg gaaaaacata acgatagcgt aaaaacaatt cattacaaac atcgactcgc
     5041 ggtgtacaaa tcgctactct tcctccagct tttaaagttt ccactaaaat aggataaagc
     5101 atctctgttt tccctgcacc tgttacagcc caaataagat gatgtctttt ttctgaatgg
     5161 caaagtattc tctttgagat ttccgtttgt ttttcagtaa gctgtcctgt ccaaaaaaag
     5221 acgacttctc gaggttttgc ttcaggctga tcaaacaaat ataaatcttg ttgagtgtcg
     5281 catcgcccta aatatagaca atgtgtacaa aaataaccgt aatctgcaag ctgtccatca
     5341 atgacggacg tcctcattcc acagcgatga caaagaatcc agttgcccaa acgttccact
     5401 gctgcgatct tctcataatt tggtaaattt acctcctcac agtctattgc ttcaatatct
     5461 ctcgtcagaa gccttctacc ccaaaaatga ttcatattct tcgccctaaa aaaatatacg
     5521 aaaaaacaag cgatctattt tttggatcgc ttgttcgata gttatactgt tctatttatt
     5581 cacctatgag cgtgattgct tgatctaaca ctttttcgcc attcatcata ccataatcag
     5641 ccatattgat gacatctaaa ggaatgcctt ttggttccag acgtttctca aaatcagctt
     5701 tcataaagcg aacttgtggg cctaataaca acacatccac gtctttattt gcaaggttgt
     5761 tatctgcgtc agaagcggac acagcgaaaa tatccgagtc gattcctttt gcttcagcag
     5821 ctttttgcat ttttgttact agtaaacttg tgctcatacc agccgaacat acaagcatga
     5881 ttgttttttt agccatactt ttctctcctt tttaaaacaa actatgcaaa cgttttcttc
     5941 gcgtctgtta ctctattata taaaaagaaa gaaatttgtc aacgttttct aaacgcggtt
     6001 tatttaatca aatagccaac tagatttcac taatttattg aacacgacat attctttgtc
     6061 caaattgatg acaactactt catagcctgc atttatccag ccataggcac ctttttcagg
     6121 gtgtttataa tcatttgccc accaatctgt actttccctt gctgttttgg gaagtccgtt
     6181 actgggaaac agcagttcgt caaattgggt aaaggtcaac gttacttgag agccgccatt
     6241 gttcttcaaa taatgcgtaa ttgcatcgta ctttttcatt cgttcctgca tccccagatc
     6301 cacctgcctt tgtctttatt atcgcatact acagcaattg cgcaaccgtt acagtgttga
     6361 aacaacctag aactttattt atactgaata aagaaaatga aaaaaagagg tgacaagatg
     6421 atcgagcgct atcgtacaat aaaagaaaat ggacaaagtg agattgagat caaaaaatca
     6481 cgctttatct gttctcttag acgaatagaa tcagaggacc aagcaaaaag tttcattcaa
     6541 gaaataaaaa aagaacattg gaaagcgaac cataactgca gcgccttcat cattggagaa
     6601 aaaaatgaaa tccagcgaag cagcgatgat ggagaaccaa gtggaacggc gggcgtaccg
     6661 atgttggaag tcctcaaaaa aaatcaactg atcaatgtag ctgcagtagt cactcgatat
     6721 tttggaggaa cgaaactcgg cacaggcgga ttaatacgag catacactca tgcggtctct
     6781 caagcattgt ctgacattgg aatcgtagaa ggtaccttgc aacaggagat ctctgtgatg
     6841 atcacgtatc cgcaactagg aaaattacag aattatcttg aactccaaca tttttttata
     6901 aaagaaatcc aatatactga tcaagttagc gtcatctgtg ctgtagatga aaaaagtgct
     6961 gaccacttta aagaatccat cattgagctg ctgaacggac aagtccaatt tgaagagggt
     7021 tctgtttctt atcacgagca actgataact gatcatatat aaaaacaggt gattctgatt
     7081 ctcttttatt tctaataagg aatcagggat cacctgtttt ttatttttcc aaataaaact
     7141 caaaacgatt tcctacgtat tgcgtacgca catactcaaa cggcgtaccg tcttcaaaat
     7201 aagaaatctg gcgcaaacga agaactgcat ctcctcgttt tacctctaaa tagtcagcaa
     7261 ttttctcaga agccaacaca gcagtaatcg tttgattcgc tggaccgatc tttttcccgc
     7321 ctttttcttc tagcgtgcga tacaacgatt cagtaatctc tgctttgctg tagccatcga
     7381 tcagcttttg tggaacactg gcaacttcaa aacaaatagg gacatcatct gcgaaacgaa
     7441 tacgttccat tcgcaagatc gtgtcatctt tactcaagtt tagtttttcc atttcacttg
     7501 agcttggcga agtcaagaaa taagagattg tccgactaga cggtactcga ttttgtgatt
     7561 ccataatttc tgtaaaactt gttgcaccag acattttttc ttgtactttt tctcgcgcaa
     7621 catacgtacc cgaaccgatc tttctttcta agattccttc atctgccaag ttttgaattg
     7681 cttgtctcaa cgtcatacgg ctgacattga atttcactgc taattctctt tctgaaggaa
     7741 gacggtctcc gactttccat ataccctgtt cgatttcatt ttttaattgg tcatgaatct
     7801 gtatatatac aggtaattga ttcgacataa cgtacacccg tcctttcgtt tcttctatta
     7861 taactggtat atacctgatt cacaactgaa aaagataaag gtttctatcc atcaacagag
     7921 gatttagaca cacatcgttc tccctactcc tattgtcaga aacaattttt tagatatgtt
     7981 caacctagaa aataacaaaa gaccctaaaa tcgatgaaaa tgccgactta tggatctttt
     8041 gttattatta gcggttttta gccatccatt tttttgtatg attcgttggg cgttttgatt
     8101 ttacctggac cgcttttccg ccttttttcg gaatattcac ttcaatattg tatttttctg
     8161 atttgaccaa atcatataac ttttcagctg cttcgtctac tttttctttc catttcatgt
     8221 ggatccctcc aattcttgta gttaacttaa ctctatctta attcagcaga atcttcaagg
     8281 aacaaggtag caaagcaaaa aaaagagctc tcgaactaac
//
LOCUS       DACCQP010000102         8253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCQP010000102 DACCQP010000000
VERSION     DACCQP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.106"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IVO87_002554"
     tRNA            8..81
                     /locus_tag="IVO87_002554"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IVO87_002555"
     tRNA            101..173
                     /locus_tag="IVO87_002555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IVO87_002556"
     tRNA            201..274
                     /locus_tag="IVO87_002556"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IVO87_002557"
     tRNA            288..361
                     /locus_tag="IVO87_002557"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IVO87_002558"
     tRNA            385..474
                     /locus_tag="IVO87_002558"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IVO87_002559"
     tRNA            487..560
                     /locus_tag="IVO87_002559"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IVO87_002560"
     tRNA            579..654
                     /locus_tag="IVO87_002560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IVO87_002561"
     tRNA            656..726
                     /locus_tag="IVO87_002561"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IVO87_002562"
     tRNA            740..813
                     /locus_tag="IVO87_002562"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IVO87_002563"
     tRNA            838..925
                     /locus_tag="IVO87_002563"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IVO87_002564"
     tRNA            947..1018
                     /locus_tag="IVO87_002564"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IVO87_002565"
     tRNA            1041..1130
                     /locus_tag="IVO87_002565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IVO87_002566"
     tRNA            1143..1216
                     /locus_tag="IVO87_002566"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IVO87_002567"
     tRNA            1235..1307
                     /locus_tag="IVO87_002567"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IVO87_002568"
     tRNA            1311..1383
                     /locus_tag="IVO87_002568"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IVO87_002569"
     tRNA            1388..1458
                     /locus_tag="IVO87_002569"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IVO87_002570"
     tRNA            1471..1544
                     /locus_tag="IVO87_002570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IVO87_002571"
     tRNA            1556..1627
                     /locus_tag="IVO87_002571"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IVO87_002572"
     CDS             1893..3086
                     /locus_tag="IVO87_002572"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7450706.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IVO87_002573"
     CDS             3216..4688
                     /locus_tag="IVO87_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7450707.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IVO87_002574"
     CDS             4771..5559
                     /locus_tag="IVO87_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7450708.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IVO87_002575"
     CDS             complement(5626..6294)
                     /locus_tag="IVO87_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7450709.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IVO87_002576"
     CDS             6409..8121
                     /locus_tag="IVO87_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450710.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
BASE COUNT         2344 a         1502 c         1901 g         2506 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcg
//
LOCUS       DACCQP010000103         8067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCQP010000103 DACCQP010000000
VERSION     DACCQP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.107"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IVO87_002577"
     CDS             <1..883
                     /locus_tag="IVO87_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7450711.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IVO87_002578"
     CDS             931..3060
                     /locus_tag="IVO87_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7450712.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IVO87_002579"
     CDS             3035..3724
                     /locus_tag="IVO87_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450713.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IVO87_002580"
     CDS             complement(4132..4818)
                     /locus_tag="IVO87_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7450714.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IVO87_002581"
     CDS             complement(4954..5148)
                     /locus_tag="IVO87_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450715.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IVO87_002582"
     CDS             complement(5198..5638)
                     /locus_tag="IVO87_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7450716.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IVO87_002583"
     CDS             complement(5642..6487)
                     /locus_tag="IVO87_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7450717.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IVO87_002584"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IVO87_002584"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7450718.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DACCQP010000104         8027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCQP010000104 DACCQP010000000
VERSION     DACCQP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.108"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IVO87_002585"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IVO87_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7450719.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="IVO87_002586"
     CDS             1229..2332
                     /locus_tag="IVO87_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7450720.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IVO87_002587"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IVO87_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7450721.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="IVO87_002588"
     CDS             complement(3822..6269)
                     /locus_tag="IVO87_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7450722.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGESNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="IVO87_002589"
     CDS             complement(6458..7894)
                     /locus_tag="IVO87_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7450723.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2231 a         1739 c         1371 g         2686 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttgattcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
     7981 ggaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaatttt
//
LOCUS       DACCQP010000105         7614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCQP010000105 DACCQP010000000
VERSION     DACCQP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.109"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IVO87_002590"
     CDS             complement(14..706)
                     /locus_tag="IVO87_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7450724.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVO87_002591"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVO87_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVO87_002592"
     CDS             complement(1143..1712)
                     /locus_tag="IVO87_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7450725.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVO87_002593"
     CDS             complement(1786..2109)
                     /locus_tag="IVO87_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450726.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVO87_002594"
     CDS             complement(2263..3075)
                     /locus_tag="IVO87_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450727.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVO87_002595"
     CDS             3429..4277
                     /locus_tag="IVO87_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7450728.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVO87_002596"
     CDS             4422..5363
                     /locus_tag="IVO87_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7450729.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVO87_002597"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVO87_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2267 a         1508 c         1373 g         2466 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 tcccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttt
//
LOCUS       DACCQP010000106         7506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCQP010000106 DACCQP010000000
VERSION     DACCQP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.110"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..144
                     /locus_tag="IVO87_002598"
     CDS             <1..144
                     /locus_tag="IVO87_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7450730.1"
                     /translation="YRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
     gene            complement(760..1800)
                     /locus_tag="IVO87_002599"
     CDS             complement(760..1800)
                     /locus_tag="IVO87_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7450731.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2149..2475)
                     /locus_tag="IVO87_002600"
     CDS             complement(2149..2475)
                     /locus_tag="IVO87_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450732.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2462..3250)
                     /locus_tag="IVO87_002601"
     CDS             complement(2462..3250)
                     /locus_tag="IVO87_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7450733.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3618..3908)
                     /locus_tag="IVO87_002602"
     CDS             complement(3618..3908)
                     /locus_tag="IVO87_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7450734.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4044..4223)
                     /locus_tag="IVO87_002603"
     CDS             complement(4044..4223)
                     /locus_tag="IVO87_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450735.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4220..4570)
                     /locus_tag="IVO87_002604"
     CDS             complement(4220..4570)
                     /locus_tag="IVO87_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450736.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4563..5888)
                     /locus_tag="IVO87_002605"
     CDS             complement(4563..5888)
                     /locus_tag="IVO87_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7450737.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6338..6952
                     /locus_tag="IVO87_002606"
     CDS             6338..6952
                     /locus_tag="IVO87_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7450738.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7117..>7506)
                     /locus_tag="IVO87_002607"
     CDS             complement(7117..>7506)
                     /locus_tag="IVO87_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450739.1"
                     /translation="KEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNF
                     FYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYK
                     ELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2300 a         1352 c         1113 g         2741 t
ORIGIN      
        1 tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat tcgaggatta
       61 tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac tgaaatcaag
      121 gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat tctttattta
      181 aaactttgca acagaaccgt ccgttgcggg taataccgta acactgaagg gcacgcatta
      241 aggcgtcatc ttaatgcttt cctgtaggac actgacggag attaccagtc tcccgagcct
      301 tacaggggcc tttctttttt ttgtattttc aattatcaca atttatcgtc caaaagtgca
      361 gaacgaaaat caattcatca caagcatatc agacaattaa atttaaaaca actaaaaata
      421 tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat atcaggcttt
      481 tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact caaaaaagca
      541 catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat atctaacgat
      601 tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta aaaactccgg
      661 aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa aagttctaat
      721 ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc aattttccgt
      781 actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct ttgccgcaat
      841 agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca aattgtttac
      901 tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc ctttgtcagc
      961 atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta attcttcagc
     1021 ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt aagtactaga
     1081 aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac tgtcctcttt
     1141 catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag tttcagtgtc
     1201 tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg cgattttcgc
     1261 aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt tcgcaattac
     1321 ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa ttacggtggt
     1381 atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta cttctggctt
     1441 gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat ttactcttcc
     1501 tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag atactttacc
     1561 ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa atatattatc
     1621 atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa gtagcatata
     1681 agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa ataccttagg
     1741 catttgataa tatttttcac gataaatttc ttttgatttg taataattaa aatcattcat
     1801 agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa tgaattaaac
     1861 aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac tttgttaaag
     1921 cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa gcaaaccgct
     1981 ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat attatatcac
     2041 gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac tactgatttt
     2101 tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt
     2161 ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg actttaatag
     2221 cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt
     2281 gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta cactacttcc
     2341 ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt
     2401 tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag cttgactgcg
     2461 attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat
     2521 agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg
     2581 cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca ccaatgactc
     2641 tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc ttattatact
     2701 tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa
     2761 tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca actggaatga
     2821 taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat
     2881 caattataat ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt
     2941 cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa
     3001 catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt
     3061 cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc
     3121 cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg
     3181 ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata
     3241 ttcttgccat taaaatatcc cccattcatc ttatattata atataattat atcataatat
     3301 tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta ttttgtttta
     3361 aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat taaaataatc
     3421 actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc gagcagcaac
     3481 tagaataatt taaacattac taaactgtct atattgttac taataattcc cttcttatag
     3541 tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta
     3601 tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag
     3661 gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta
     3721 gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg attttattct acgttgtcgg
     3781 gttatccagc ttgcaaccgt tccttgtggg taggcatata actcacaaaa ttgttctaca
     3841 gtgattccta aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg
     3901 atagacatgg tgcgtaagcc tactttcttt tattattgcg tttgcaataa tagtttaaca
     3961 cacgcattat tctagtatct actcctattt gtaaaaacaa aaaaggattc atagagataa
     4021 ctctatagaa tccgtaaaat accttatata tataacggga agatataaaa cctaaaatta
     4081 tgataacctg ctgcagcttc tacatagaaa gagaagcgct caacaccaac ctcatttatc
     4141 aaacgttggc cataacccca aatctcttct gatagctgat caaaggttat atactttagc
     4201 tcatattgct ggataatgtt cattctaatc gactccattt attatcctgg taaaattcga
     4261 tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac
     4321 tacctaagat cttgccaatg atatcttctg gatagtatcc ttcagcgttt cgttcttctt
     4381 tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt
     4441 cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa
     4501 tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt
     4561 aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta
     4621 gtaatgcttc tagcgccttc taacattgaa gaagcatgta caatcgacgt aaaaccatat
     4681 ttttgccgaa tcttgtcaat aattttatct aaattttctt cgtcaatttg cttttgtgga
     4741 tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca
     4801 acatgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc
     4861 aattcctttg tattatctgt gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt
     4921 tctaagatcg acgtgccaat gtgtaaatgg acacaacctg ttttacagtt atgctgccta
     4981 atgcgtatcg ctacttgttc cgccatttct tttaccacca gctcgatttc atttcttcta
     5041 gtataatctc gtggtaacac ttgtgaattt ccatatgatt tttcctttgt cggttctggt
     5101 gggattgcta tatctgttcg atcgatccca ttagcatgga agtacagctg taagccaatc
     5161 acacctagtc tattcttaat cgtataagga ttccagttag caagatctct aatgctcata
     5221 atccccatct gattgagtct ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt
     5281 tcaggaatat tccatacttt ttcaggaacg ttttcgtacg tccattccgc aacaaatcct
     5341 tcattatgct ttgcttcgtt gtctaaggct agtttagcca aaaggggatt atctccgaca
     5401 cctactgtag caatcaatcc tagctcttct ttaatattct cctggatcat ttgagccagc
     5461 ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa gattcagtga tcgggtcact
     5521 tttaagattg attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg
     5581 aaaatattat ttacctgcat atttcgcttg atatataggt tcatacgtgg tggaacaacg
     5641 tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc tactcacatt tgtaatacca
     5701 taccgctttt ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attatcagct
     5761 cgactcataa caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca
     5821 cttgcgtaaa agcttttcat atcgattaaa aagtaatcat ttactggctc ttttgaataa
     5881 tccatcataa ataacactcc attctttcac attatacaaa catatgttcg atattgtaaa
     5941 gaagaaatag ctaaatacat aaaaaaagaa gcataggtat gctatcaccc tgcttcttct
     6001 tttgatttgt atgatcaatt tattctatca ctttactaat taatttgaaa atctaataaa
     6061 aactctcact ctaaaagagt gagagactgc aaattagagt acggactttt ttggagttcc
     6121 gttctttaat attaacaaat gaataaaatc cttacaacat ttgagaataa gttatcagta
     6181 gacacttaca aaaaattctc atactatttc ctccatcatt agtttttgtc tatataaggt
     6241 ttaccctaaa tagacactgg tataacattg aaaaatatgg cttcctcgtg tatgattagg
     6301 gtgtatttta aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt
     6361 ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga
     6421 gagaattttc atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga
     6481 agcattagat tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg
     6541 aagaaattat gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtaaagct
     6601 gatcattact agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt
     6661 cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc
     6721 gaaacgtcgc caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg
     6781 gcccaaatta tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat
     6841 cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac
     6901 gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat
     6961 gctgctttta ttatcttctc atctttaatt tcctacatat aattactatc tataaaatta
     7021 agtcttactc agaacagata aagcataaaa ttccaacaaa ccatttttta cttaactaaa
     7081 taaagagtct gttaatataa tcttcactta tttacgttaa tcccctactg tatttgttgg
     7141 ttccttatct atattttcta caaaatcctt attatcttgt gatttagctt cgtataattc
     7201 tttgtatgtc gacacattgg ttatactaat tcctatagct agttcttgag actctacttt
     7261 tctctctagc tcctttgaca aagtgatttg agatttattt ggttccgttg gagtcaattc
     7321 tatagattct tcataaaaat ctctatacat aaagctatca ttaattttat aaaaaaaatt
     7381 aaatgttttt agcactgcca tataacattc aaaacattct tctatagttg gtttacttcc
     7441 ttgaggagaa tgaaccaatt tattacgttt ttctatgaaa tttttaattt cctttcttat
     7501 ttcttt
//
LOCUS       DACCQP010000107         7235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCQP010000107 DACCQP010000000
VERSION     DACCQP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.112"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(181..441)
                     /locus_tag="IVO87_002608"
     CDS             complement(181..441)
                     /locus_tag="IVO87_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450740.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(578..991)
                     /locus_tag="IVO87_002609"
     CDS             complement(578..991)
                     /locus_tag="IVO87_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7450741.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1447..1932)
                     /locus_tag="IVO87_002610"
     CDS             complement(1447..1932)
                     /locus_tag="IVO87_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450742.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2071..2286)
                     /locus_tag="IVO87_002611"
     CDS             complement(2071..2286)
                     /locus_tag="IVO87_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7450743.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2510..3310
                     /locus_tag="IVO87_002612"
     CDS             2510..3310
                     /locus_tag="IVO87_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7450744.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3329..5197
                     /locus_tag="IVO87_002613"
     CDS             3329..5197
                     /locus_tag="IVO87_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7450745.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYNILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5190..5681
                     /locus_tag="IVO87_002614"
     CDS             5190..5681
                     /locus_tag="IVO87_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7450746.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5669..6223
                     /gene="srlA"
                     /locus_tag="IVO87_002615"
     CDS             5669..6223
                     /gene="srlA"
                     /locus_tag="IVO87_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7450747.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6241..>7235
                     /locus_tag="IVO87_002616"
     CDS             6241..>7235
                     /locus_tag="IVO87_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7450748.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2552 a         1161 c         1385 g         2137 t
ORIGIN      
        1 taaaaacaaa gctttactca taactaacaa aataccagct ccttttattt ttttctcccc
       61 cacattcaaa gagtaacaat ggtatgtact taataataga aaaatggatc aacttgtatg
      121 aataacagct gaaaagatct gcaccaaata aaaaacttcc agccaaaact gactggaagt
      181 ttattttttt ctgaaatatt ctaacacaaa tatgaaaaag atggccgaag ctaataagcc
      241 gatttcgtag caaaacaatg agataccaga gtcgtaataa taaaatcctc taatcttatg
      301 gataaaatag atcatcggac aagcagataa gagtaagtat ttgttaggcc ttttgaataa
      361 gtaaaagaaa gcactggtca ttatgataaa tggtatccaa aacaaaagtt tacttaagac
      421 aatcaaatga tcaactccca tattttttac caagagagat cataacatct ttatcgtaaa
      481 aacaaaatat ttttttgctc attttgaaaa tttaaacata ctcgttctta tccaaatata
      541 taaaaatcgc ccctcattca tcgagaggcg ataagttcta atttaactca attttttttg
      601 tttcttttgg ctgataagct gttttaggta ataccaattt cagcacaccg tttttaaagg
      661 ttccagtgat tttttcttca tcaacattag gaagatagaa tgaacgtttg taagagaccg
      721 aatgtctttc tttccgtaca tagttgccat cttctttatc ttctgtatgg gactcatgat
      781 tggcggagat cgtcaaagta ttatctgaat attcaactgt cgtgtcttct ttgtcacagc
      841 caggcatatc agcagttagt ttgtattctt tttcattttc gactaaatct acttttggat
      901 aacttgaaac acctaaaaaa tcattgaatg ctggactaaa cacgtcattg aaatcaggga
      961 acatgtcgcg aatgctaggt acatttgcca tttttgatca tcctttcaat aagtattttt
     1021 tatttatccc tatttacgta ataactctat tttgaaaaaa atgcaagtat aatggttagc
     1081 tttgtgaata tttcatctaa tacgatacaa aggctatctt tctaatttga aaaagggatc
     1141 attaaaataa taagaagaaa atatgtcaaa aggtaaaaaa gtaaattctt ctatccaaat
     1201 atagaaaaat tgaatctaga aaaccgcccg acaataaatt tcattaactc agttgaccga
     1261 aaatgaagta gataccaaaa atttatcaag agagcaagta gagaagtgag tatattcata
     1321 agagaaatag ctgtaacgaa ttattaggtt aatgcaaatg cttatttata ctccgtataa
     1381 ctttctcaga aaaaatgata ccaataatga cttgctagta ctgtaaatcc gtataatatc
     1441 atctaatcaa aatggtgttc catttgcatc tgtcatcgta atcgatcctt cgggtgacac
     1501 tcgataaaaa ccaacgcttc cagatcctcc ttgggaacgg atttgtttgc tggaagctgt
     1561 gaacaaataa tctcgatcaa ttttttgata aaagctaata acaatatcgt cactttgtaa
     1621 ttcggaacgg tgggcaataa gaaatagaag tgctttgtca gagttttcta atggaagaga
     1681 aggtacagtg ctttcttgca tttcagatgt tgaagaaatg ctaaacgact gaatacttga
     1741 ttggctatgt tgcgaatcta cattaccagt agaagtatta gtaatggaag tagtagaagt
     1801 acttttagtt gaaccaacct cagttgttga aatggctgct gaagattctt taacagaatt
     1861 ctttgtttta tatccacagg cagaaaaaag tattacagtt cccaacaagc ctgcgtaaat
     1921 ccctttatgc atgatagctc ctcctaaaat agattatctt tattcagtat atcacttaaa
     1981 caaagaaaag cagatggttt aaaatgaaaa aaccttccca ataaagggaa ggttcatgaa
     2041 gataataact tttttaataa ttatagcttt ctaatatctt tcatcaactt ttctagtaat
     2101 cattgggaaa aatttcaagc agtagatcgc acaaatacct acgacgatat aaatgatctt
     2161 cgcaaaaata gttgttgaac caccagcaat catagctact aaatcaagtt cgaacaagcc
     2221 taccaacagc cagttcaacc ctcccacaat caataaagtt aatgcaatag catctaaagt
     2281 tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat
     2341 tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc
     2401 tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta
     2461 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2521 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2581 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     2641 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     2701 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     2761 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     2821 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     2881 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     2941 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3001 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3061 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3121 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3181 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3241 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3301 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3361 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3421 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3481 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3541 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3601 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     3661 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     3721 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     3781 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     3841 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     3901 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     3961 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4021 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4081 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4141 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4201 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4261 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4321 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4381 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4441 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4501 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4561 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4621 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     4681 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     4741 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     4801 agaatattta aacaaattag tcgctccttt gatcagaggc tattatgtgg atgaagaatt
     4861 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     4921 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     4981 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5041 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacaatatcc tatttgaact
     5101 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5161 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5221 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5281 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5341 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5401 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5461 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5521 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5581 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     5641 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     5701 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     5761 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     5821 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     5881 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     5941 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6001 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6061 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6121 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6181 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6241 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6301 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6361 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6421 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6481 atttatccta aaaaagggat accaaccatc aatatcgttt ccacaggaaa aagtggccca
     6541 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6601 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     6661 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     6721 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     6781 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     6841 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     6901 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     6961 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7021 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7081 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7141 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7201 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DACCQP010000108         6975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCQP010000108 DACCQP010000000
VERSION     DACCQP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.113"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(361..1479)
                     /locus_tag="IVO87_002617"
     CDS             complement(361..1479)
                     /locus_tag="IVO87_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450749.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1950..3257
                     /locus_tag="IVO87_002618"
     CDS             1950..3257
                     /locus_tag="IVO87_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7450750.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3376..4575
                     /locus_tag="IVO87_002619"
     CDS             3376..4575
                     /locus_tag="IVO87_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7450751.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4602..5870)
                     /locus_tag="IVO87_002620"
     CDS             complement(4602..5870)
                     /locus_tag="IVO87_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7450752.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6033..6679)
                     /locus_tag="IVO87_002621"
                     /pseudo
     CDS             complement(6033..6679)
                     /locus_tag="IVO87_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2112 a         1457 c         1242 g         2164 t
ORIGIN      
        1 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
       61 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
      121 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
      181 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
      241 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
      301 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
      421 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcagca cttgacgccg
      481 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
      541 taaatgtgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
      601 tttcttatct aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
      661 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
      721 aacgtctgca caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg
      781 taatgcaaat aaataaatcg gccattgttc aggattttcg aaagaatcct tcaagtacgt
      841 atcaacagct tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac
      901 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
      961 gccacctgtc agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag
     1021 agatacttct tttacttgtt tgttgctgac ctcatacaat ttcatggagt cgcggttcaa
     1081 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
     1141 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtctg
     1201 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc
     1261 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc
     1321 aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc
     1381 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga
     1441 agtcaattgt gtcaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
     1501 atcattcctt tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg
     1561 tatctaaaaa aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct
     1621 tcataatttc ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa
     1681 actaagaaaa agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt
     1741 aagtattatc agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc
     1801 tattcctgta aaagtacgta aaaacgaccg ctaaacttcc ataacgcgcg ttcttatcct
     1861 aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc
     1921 gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat
     1981 tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg
     2041 aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa agcggtaaga
     2101 ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg
     2161 gtctttacat cgtccttgat caagtaaaag aaggaaaact gtcatggggt gataaagttt
     2221 cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat gttcctttgc
     2281 ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc cagtcagcca
     2341 atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaagctaaa ttcactgaac
     2401 gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacaatcgtt aatgcatcag
     2461 gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg cactggagaa aacgacgaaa
     2521 atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg gatttcccag
     2581 aaatactgga tgtatcaagt actacgaccc aaatgtttgg cgaaaatacg caatcaccag
     2641 tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag gaaggcgtgg
     2701 acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt acgatcacta
     2761 aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg gaaaacccga
     2821 gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat tggtcagaaa
     2881 aagagctcgg aaaagctaac gcaagtattc ctagtctaaa aaccattgat gtaaaagacg
     2941 gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg gtacgaaacg
     3001 atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa gtcactgaca
     3061 atgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg atcaccttgg
     3121 cagacgataa attaggctat ttagaagaca acaatgcacc atcaacagag atcattacgg
     3181 ataaaacagt ggaaaaagcg aatatattcg tgattggctg gcgacgcgtc gcagaatttt
     3241 tcggcaactt gttttaattg aacaataaga aaaacatctt tccatcggaa agatgttttt
     3301 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     3361 aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct ctatcttatt
     3421 ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt gttcttttac
     3481 ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga aattttttat
     3541 tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt ttcttatttc
     3601 acagaaaaac gaaggctact gaatggtctt ttattcaatg ttttactcat cagtttcggc
     3661 atctacttgt ttgtcttgct gtatgaaacg caaaatattt ttcttggcgg actgatcgcc
     3721 ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt gattgttttt
     3781 cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc taatctttta
     3841 acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt cttgttaaaa
     3901 tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt gatctatcta
     3961 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccggcctcga
     4021 tacaatcaag acttcatcgt tgtgttaggc gctggattga tcaatggaga aacagtttct
     4081 cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca aagccgtgct
     4141 acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga aaaagtacca
     4201 gaggccattg cgatgaaaca atatgccatg gaacaaggaa tccctgaaag agacattctt
     4261 gtcgaaacca attcgacaac cactttggaa aacatgctct attcaaagga aatcatggat
     4321 cagcaaatga aagggccata ccgcgcgatc tttagttcca acaattacca catttttaga
     4381 gccggtcttt atgcgcggca agccaaattg aaagcaaatg gtattggtgc aaaaacagct
     4441 ttctactact tgcctaatgc gtttttaaga gaatacctcg ccatcttgat gatgaacaaa
     4501 aaaagacata tgatcatttg cggattgctt ttcgcaggct ccgcattctt atcgctgatt
     4561 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     4621 ttcattctct taactattgg caaaaagagt atcccttttt tgaccgctac ttgctgagca
     4681 gaataaatac cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat
     4741 actgccgctt gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt
     4801 gtcgcaccag aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg
     4861 agcaaaggcg ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct
     4921 cccccttgat atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca
     4981 tagacatgtg cctgtccatt aaatgattct tgaagtaacg gaagactcaa tccgagataa
     5041 cgttgtgttt gcaaaatcaa tgctgctgcc acaactacag ctccacctat cgtatttcga
     5101 caaacaggat gaggaatcca ttgtgcatat cgcgctttta aaaagacgat cgaacggctg
     5161 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     5221 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     5281 gtcatgaaat ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact
     5341 ttcccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca
     5401 gaactaaaac ccgcactgat tccactgata atcaagattt cccgttctga tttatccaat
     5461 cggaacaaac gtgccgtatt atctgctaac gctcccccca tttggactgc tgttccttca
     5521 cggccaacag atcccccaaa taaatgggta gttatggttc caaacagtgt aagtggaata
     5581 agccgcaacg ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca
     5641 cgactagaaa gtccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt
     5701 agtagacaaa ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt
     5761 aagaaaaaag cagacaagct ccccatgaat ataccgagaa tcgctgtgat caccagccac
     5821 ttcagcatat acactaaaat gcgtactgga ctcggtaatt gtttttccat aaaaaattcc
     5881 tcctcaataa aaaaacgcct actaaagaaa ggggctgtcc ccagacttta gtaggcgtca
     5941 ttagctatct ttcgttagca tttaaaggcg aacaccatca cctattcact tccattaaaa
     6001 gatagcatat ttttctgtaa aaaacaataa aattatcgtt ctttctttac acgttcagca
     6061 aattcataga aaggagccaa tttcgttaac tgttgattac attggtcact atcatgacaa
     6121 atataattcc cctttttcgt atacgtgcca tcagaacctg ctttcgttgt agaaagaaaa
     6181 agcgaaacat tcgatgtttt ctgacagatt gcacagattc ccttatttat cgttggtgat
     6241 aaagtaccgc taatcccttt taatttttca tcttcgtaat agaccatgaa ttttttctgc
     6301 gtacctggat cattccagcc gatgtacgta tgttcttgca agtcgatttc attccaagct
     6361 ggttgtttga gttttttcac ttttcggaat aatttctcga tttgtggttt gctaggctgc
     6421 ataaacggct gaacgatttg cttcagctcc tccaatgctc tttctgcttg cgcttttgtc
     6481 agttttacgt ctaaaactgt cgttaaaaat gtatcgattc tggcagttcg tgatctaata
     6541 tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt ggatcattga
     6601 cagatgagta cgcatttaat aactgttcta cttctcttat tataaaaaaa tattggtatg
     6661 gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
     6721 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
     6781 agatacgttt tgcatcttat catcaccccc tttaatgctt attattctat gtacatgatt
     6841 cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa acagaaaaaa
     6901 ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctg
     6961 gcatctgcgg taata
//
LOCUS       DACCQP010000109         6569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCQP010000109 DACCQP010000000
VERSION     DACCQP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.114"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..636)
                     /locus_tag="IVO87_002622"
     CDS             complement(<1..636)
                     /locus_tag="IVO87_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450753.1"
                     /translation="MQAPIKKICVHFETKQWRNKMVATVTLYLTFTLILDLGIYLFFS
                     KQLLVKNSFGCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFF
                     AITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPT
                     EHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLSIVISFFSLFFSIMLI"
     gene            637..1377
                     /locus_tag="IVO87_002623"
     CDS             637..1377
                     /locus_tag="IVO87_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450754.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1463..2221)
                     /locus_tag="IVO87_002624"
     CDS             complement(1463..2221)
                     /locus_tag="IVO87_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7450755.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2218..3246)
                     /locus_tag="IVO87_002625"
                     /pseudo
     CDS             complement(2218..3246)
                     /locus_tag="IVO87_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3266..6433)
                     /locus_tag="IVO87_002626"
     CDS             complement(3266..6433)
                     /locus_tag="IVO87_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450756.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
BASE COUNT         1854 a         1390 c         1157 g         2168 t
ORIGIN      
        1 gatcaacata atggaaaaaa acaaagaaaa aaaactgatg actatcgaca aaagagatag
       61 aagcagataa ttagatgagc ggaagtgaac acttcccaat accaagtaaa tcacgcccca
      121 gcttaataaa atacaaatac ctccaaaata gtgttcagtt ggataagtaa aaagcaagtt
      181 gagaaatctc gtctgtttgt ctatactttt tactaataga tagaaaataa atgcgataag
      241 tagtgcaaaa aaacttgctg ttccaagatg tctctttcct aaataatcca tcagatcaaa
      301 aaccagaaaa aagctgagtc cagtgatagc aaaaaaaaga ttactgatca aacagctgat
      361 aaacaggttt ttcgtagtaa aataaaaaag aaacatcaat aataagccaa ctaggtaagg
      421 aaagctcact ttagggatag gaagtaaaag tgtcaccacc atcaaagcta tacaaccaaa
      481 gctgtttttt actagcagtt gtttcgaaaa gaataaatag atacctaaat ctaaaatcaa
      541 agtaaaggtc aaataaagtg tcactgttgc gaccatctta ttcctccatt gttttgtttc
      601 aaaatgtaca catatttttt taataggagc ctgcatatga aaaagtattt tatcatagaa
      661 gataaccagt ttcacagcga atggatcatc agagaattgg tcaatcatca tatcgagaat
      721 atcgtgcagc tcacttctat ctctcaaatc aatcagttga tccagttggg cactattgat
      781 aatcacgatg ttttctttat cgatatcgac ttgaagcaat cactcacagg tattgatgtc
      841 gccgaacaaa tacgtaaaaa aaatgcacat agtaatattg tttttttcac cgcttatacg
      901 aattttgcag tagagatcat caatcggaaa atccttccga ttggatatat cagaaaagac
      961 aggaatttat ctgaacaaat tcgtcaagtg ctagaacaga tcaattggaa agaacagcag
     1021 atcgtaaatg tcgataaaat ctttattgat caaaaaggtg gaagtctcgt cctgtatcct
     1081 cgggaaattt tatatattga aagtatcaaa ggtttgaaaa accaagttat ggtcaaaaca
     1141 actacagaag ataaattagt gaatacttct ttaaaacaat tgaagacaga gttagaaaag
     1201 tatgcttact ttttactttt aaaatcttac tgtatcaact tatcgcaatt gcagaaaatt
     1261 gattctgctc attatgtgtt agaatttttc aacggtgact ccttactcgt tggtcgaaag
     1321 atctttgaaa agacaaagga acgtttccat gacttccaaa aaactgcttc gagttgatga
     1381 ataagagctg agacgtaagc atagcagatt tcagggagtg ggccttgaat cggctccact
     1441 ccctgaaatc gttgatatga ttttacgctt cctcttctgt aggagcatct gttaacgacc
     1501 atgtgaatgt tgaaacgtag ttgtctacag cataattgga tacaggaaca aataattcca
     1561 ctgattgtct cccttgttcc acattctctc ctaatgaata aatccatgtg ccaatccctt
     1621 catttgctcc tgcagttact agatctactg cctgatctgc tgggatttcg attccttcag
     1681 cgccagttcc agaaacggta gatggcattc cactgctatt agttgcgcta tttcgaatca
     1741 cggcgttttt gaatttcacc tttgaaccag ttagtttatt cgtatcagat gttgtaccaa
     1801 taaattctgt tcgtttgact gataatttcc aaccggcata atttccgctt ttatccgtta
     1861 cttgtacgaa attctgtact tgtgtttgtg ctccagtact gtccgttccg ctttgtaatg
     1921 cagcatatac tgtttttgct gtacctaact tgattgttcc aaaatcaaga tctggagctt
     1981 ggtcaatcgt cagtaatccg gatgttccct gatttggttc gtcaggtgta aaaggagtat
     2041 caggatcatt tggatcaaca ggaggaactg gaacaacgtc ttcaggattt gcagcatctg
     2101 gtttggtcac agaaatcgtt ccttctgtag tggctgagct cttattctca gcaaaaacac
     2161 tttgtgtaca taataataga cttccagcta gaacggtact taatgcaatt gtttttttca
     2221 tttctttttc cttctttctt ttaaaataaa taatccagca aataagccaa taagaatgac
     2281 tccaacaagt atcagccatc gccagtcaaa tgtttctggc tgatttttta aatcaatatc
     2341 cttttcattc aattggtccg ctgcatcatc agttatttca aaatcggtat ggagattcca
     2401 gcgtttttct ttccaagcac ccttcacaac cgcttgataa tgtcccggag ctaatttttt
     2461 tccttctaat gggacaagta aatcaaaatt tgtattgggt gctacatcta ttttttcttt
     2521 ttttattgaa aggtaaggct cttttgtttc ttttttgtag atttcaaccg atgtagtcaa
     2581 gccgaacata taagaagcat ttggattttg aagattcgcc ttaatcacat ttcttccatc
     2641 gctttgatcc gcataagctt tttttaattt caaatctggg gaagactctt tttcgctatt
     2701 tcaaatcaag attgctttca cttgagaaaa ctgattgacg acaccaactg tacttttgtt
     2761 agaaacgttc tctttttttt cttgaaacgt gatccctcct gcgaccacac cagccagctc
     2821 tttttctggc gctttcagtg aaagtgtctc agtgaccgca ctttgaggag gaacagttat
     2881 ttctcccact gtttttgcta agtttttcat atcataactt aatgtttcat caaatttact
     2941 ttttgattct ccatattcga tcacaccgtt tgaattcgtc gtagcggagt ggatcaatgt
     3001 ctcaactgtt actgatttat ctgttcggtt aaagaccgtg taggggattt cctgtgtttt
     3061 cccaggctcc atccttacat ttatatatgt tttgttctta tcaatctgat tagccggtaa
     3121 ttcaaaatct accgcaaaat tgaattcgga ggcatggaca ggtgtctgcc aaaggatgaa
     3181 tccaaggaga aggatcataa gtaaagagca gttgtttcga tgacttttat tccatattcg
     3241 attcatcaat catcctcctt gatctttatt ctgtcggcgc atctgctaat gtccatgtta
     3301 tctctgctga gtaattacca gctatctgat tttctttggt taagacaagt ttaaacgccc
     3361 gaccgttatt taaatattct gaataatctg cttgcacatt cgttgaattt tgtcctctga
     3421 caatttccag ttcttcttca ttcactaatt gatactcctc tcctttcttg aaatatagga
     3481 catcctgcat agaaaatcct gcatcatttg tgaaaggctg agataatttt atcgttaaga
     3541 tccacggatt tttcgtttct cgctgatcat aaatccataa tttttcctgt atatccattt
     3601 ggccaaatgc ttcttgtgtc aaaccagttg ctttgatctg accaaaatcg agtttggaag
     3661 gggcaagaaa tgagagacca ccttctacta cgatcttcgt agcaggtgca aaaggcgggg
     3721 catcatgata aacaactggc gtatcacttg gattgtgatt cccaatttca tcgttggtac
     3781 ttggcttgtt ctgcacaccc ttagcagtag cttctcctgc ttggtcgttt aatacaattt
     3841 ctaatacatc tcctgcaaaa agttgatttt ctgggataga aagaacaaat tcccctgtat
     3901 catcaatagt tgtttctgac aaaacagctt gatttttttt caatgaaacc aaggctcctg
     3961 cttcacctgt acctgtgatc gtgcgagaag ctgcattgat caatgtggta cttaaattag
     4021 caggttcagg aggaatcaca tcgactgtcg ttttggcaga gtccagttct gtcacttctt
     4081 ggccacctct tactttttta gcagcaacaa cttgcacttg atcctccgtt tttaatctct
     4141 catcattggg tacttgaact tcgaacaagc cgccttgttc gccttctccc catgggttat
     4201 atcctgcccc ttcattttca tctgtttgtc cttgcgtgag agcagtaatt tctacagatg
     4261 atttcgtcgt atctgcgtaa gtaatacgta aagtcacttc tacttcgcct gtccaagcat
     4321 ctcttggctt tgcctcgaca ccttcgggaa caacgacatg cccatagatt tttttatctg
     4381 cattcgttgg cactcttaag gattcaatca tcgggagctg attattggca ctgattcgat
     4441 tataattttt cattccggca cctgtctgag caaattcatt tgttaaacct gcactagggc
     4501 ttgatacgaa attgcctaag tcggtcccat tgatcgataa gtctgtcata tcagaaaaat
     4561 aatccgggtc tttttcaaag ttatcgaatg ttgcgcctgt ttttcgccat aaagcgattt
     4621 ctgaatgggc gatctttaaa gaagaatctc catttccttg gaatacccag ccgcctatgc
     4681 cattggtctc gcttccaatc cgtttgtttt ggaaatcaaa ataggtagga gattctaagt
     4741 ccacattcaa cacatcccca ctgatgatgc cggtcttatc ttcccctgtt ttcccgacca
     4801 tgactaaata agttttttca cctgccgtaa aatttaaatc aatatctcca gttgtatcta
     4861 cagttgctcc ggaatctgcg cgtatatcga tatttgattt ttctccagtg actgtaaaat
     4921 cagctgaacc tggactttct agaaaaccag aagcgtcata aaattggatc gcttgatttc
     4981 cttgattgag tctgttcacg taagatttgc catcacctgt atttcttacc ctcaatgagc
     5041 ctccattggt tactgtcagc ttttgcgtac cattctcaaa acgaactgcg ctggaacgcc
     5101 cactctgctt atcgatgtcg ataactgatc cggctccttc tacgatcaaa gaaaggcctt
     5161 ttcctacaaa acgcatccca ttgttcccgg gttgatcgtc atctccagcc attttgaccg
     5221 agatttgtcc accacttgat acatggattt gactagaatc tccttgcaag cgaaatccgc
     5281 ccgtagttgc tgtattgatg gtgattttcc caccattcga tacagtgact ttcgatcctg
     5341 aacctctcat ttggatgatt ccgcgattgt tgtcattggt aatattgatc tttgtattta
     5401 ccgaaaattc gcttcccgaa ccactgactt caaacatgac attaccaggt agcgtatcga
     5461 ctgcctctcc ttcatcgcta acgccatctg cccgatcata atcaagagag acctttgaac
     5521 cgcttaagat atggattcct ggcgctgttt ttccactacc agtctcatct cctgaatcta
     5581 gaaaaccgta gaatttccct agatacggtc cataaagttg actaccattg atcacattca
     5641 attgatagct tctacaaaaa taggctttac ccggagcggc attcagtcct tccttcactg
     5701 gttggacatc caacacaccg gaaacaccat ctaatgtgac agttgcacga ggagcgtaaa
     5761 tcagtcctaa tttggaccca tcatacaaat caagtgtccc ggtaaatttt atttctcccg
     5821 taaaatgata tctgcttccc caaaaatcgg tcatataagc aatgataatg gcatttccat
     5881 cgatatttgg tgtcaatcca cctggatgtg cagctgttcc tttaaaattc tggaactcga
     5941 tcgactgagg tatctcattg atataaatat ggtgtgtgcc gaaattgtga gcgaatccac
     6001 caaaatcaat aacgatatct cgtttgatct ctaattccct gtctaaagtc aaaggctgaa
     6061 tcagtcggat cttagttact gattcgtctg cgagaagctg cgccagttct tctgttgtgg
     6121 cagcttcgct gtacccatcc gctgaaacag ctcttgccag tgccgcttca ctgctatcag
     6181 acgtttgttc agctacttct gaggtctcac ttgtcttatt ttcttcttgc cttttattag
     6241 tagaagcctc actttgagat tctgtagttt cagaagtaat cgattcttct gaaaaagtag
     6301 tcttgtcaaa actttgattc tctgattgcg ttgattcgtt tgttttctct acatcggcaa
     6361 aaacggatgt caccatcaag agagggaaga caaacaaaat cgacgcaatc agctttttta
     6421 cacattgatt cattctccac catcctctct gacgttttga caattttatt ttatcgtttt
     6481 aatctatttc caattgtact ttcatattta aaatatgtat ttttatggaa aaatttttct
     6541 attaggtgta aatttctttt ttgtacaaa
//
LOCUS       DACCQP010000110         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCQP010000110 DACCQP010000000
VERSION     DACCQP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.116"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IVO87_002627"
     CDS             complement(543..2048)
                     /locus_tag="IVO87_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7450757.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVO87_002628"
     CDS             complement(2045..2731)
                     /locus_tag="IVO87_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7450758.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO87_002629"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO87_002629"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7450759.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO87_002630"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO87_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7450760.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IVO87_002631"
     CDS             complement(4752..5321)
                     /locus_tag="IVO87_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7450761.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IVO87_002632"
     CDS             5507..6409
                     /locus_tag="IVO87_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7450762.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT         1833 a         1342 c         1063 g         2194 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt tt
//
LOCUS       DACCQP010000111         6286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCQP010000111 DACCQP010000000
VERSION     DACCQP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.117"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IVO87_002633"
     CDS             <1..177
                     /locus_tag="IVO87_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450763.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IVO87_002634"
     CDS             190..690
                     /locus_tag="IVO87_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7450764.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IVO87_002635"
     CDS             787..2634
                     /locus_tag="IVO87_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP7450765.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IVO87_002636"
     CDS             2637..3533
                     /locus_tag="IVO87_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7450766.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IVO87_002637"
     CDS             3583..3972
                     /locus_tag="IVO87_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7450767.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="IVO87_002638"
     CDS             3959..>6286
                     /locus_tag="IVO87_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450768.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DACCQP010000112         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCQP010000112 DACCQP010000000
VERSION     DACCQP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.118"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IVO87_002639"
     CDS             725..811
                     /locus_tag="IVO87_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7450769.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVO87_002640"
     CDS             complement(932..1621)
                     /locus_tag="IVO87_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450770.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVO87_002641"
     CDS             complement(1628..1906)
                     /locus_tag="IVO87_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450771.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVO87_002642"
     CDS             complement(1866..2780)
                     /locus_tag="IVO87_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7450772.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVO87_002643"
     CDS             complement(2762..3142)
                     /locus_tag="IVO87_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7450773.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVO87_002644"
     CDS             complement(3592..3879)
                     /locus_tag="IVO87_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7450774.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVO87_002645"
     CDS             complement(3899..4123)
                     /locus_tag="IVO87_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7450775.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVO87_002646"
     CDS             complement(4763..5296)
                     /locus_tag="IVO87_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7450776.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVO87_002647"
     CDS             complement(5317..6159)
                     /locus_tag="IVO87_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7450777.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCQP010000113         6090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCQP010000113 DACCQP010000000
VERSION     DACCQP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.119"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..390)
                     /locus_tag="IVO87_002648"
     CDS             complement(<1..390)
                     /locus_tag="IVO87_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7450778.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKD"
     gene            599..1318
                     /locus_tag="IVO87_002649"
     CDS             599..1318
                     /locus_tag="IVO87_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7450779.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            1344..3935
                     /gene="mprF"
                     /locus_tag="IVO87_002650"
     CDS             1344..3935
                     /gene="mprF"
                     /locus_tag="IVO87_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7450780.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(4123..5310)
                     /gene="tuf"
                     /locus_tag="IVO87_002651"
     CDS             complement(4123..5310)
                     /gene="tuf"
                     /locus_tag="IVO87_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7450781.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            5678..6034
                     /locus_tag="IVO87_002652"
     CDS             5678..6034
                     /locus_tag="IVO87_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7450782.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         1763 a         1155 c         1149 g         2023 t
ORIGIN      
        1 atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt gtttctttgc
       61 ttctactatc atttccgcca aatatttgac accttgatca atactgtcag ctggattatc
      121 aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg tcccttttcc
      181 ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca tatgataatt
      241 ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg aacgataagt
      301 tcttcctcct aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat
      361 acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg acatcttttt
      421 ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt gtacgcctta
      481 agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt tgtttttcag
      541 taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag gtgattatgt
      601 gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact atgaaaagag
      661 cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc gctttttttc
      721 agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata gccgaggaca
      781 tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg aggatctgaa
      841 tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta gtgatggagc
      901 aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa
      961 ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca gtaatattca
     1021 ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa atcttttagt
     1081 tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa aaatcaactc
     1141 ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat
     1201 agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac atgaattagc
     1261 tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg aaacataact
     1321 attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg aaaaaccacc
     1381 taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc ggtgaattga
     1441 tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc actatacctg
     1501 tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct atggttggtt
     1561 atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga
     1621 caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt gttagtgtgg
     1681 ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc caagcattat
     1741 cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt tcttttttac
     1801 ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg ttgatcggcg
     1861 gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa ggattccttg
     1921 gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg gaatggaccg
     1981 gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt gatctgtggc
     2041 aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg attccaggag
     2101 aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt gtgccaagag
     2161 aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt ccgtttttga
     2221 ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt tattctggta
     2281 tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta ttatactttt
     2341 ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag tttcgctggc
     2401 ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt atactattgg
     2461 gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc
     2521 ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat tttagtttca
     2581 caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa aacgaattat
     2641 ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc attattggtg
     2701 cactgtcctt gctttacatc gttattgggg tatacaatct gccagatttc cctcatcggc
     2761 gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt tcgggattgt
     2821 tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt caaggagaaa
     2881 agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt acaacttatg
     2941 gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt gcctatgaaa
     3001 aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa tgtattgtaa
     3061 tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct tttatcgaag
     3121 aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag attgttatga
     3181 ttttgcatga attcggctac gatttcatta agatggggga agaagcttat gtagacctga
     3241 atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg aatcggatcg
     3301 aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag caaatgagta
     3361 ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg ttttctttag
     3421 gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa aatacccaag
     3481 aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa gtaggaacga
     3541 ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat ttcttgttct
     3601 tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta gggatggccc
     3661 ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt gcatacttgg
     3721 tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat aaaagcaagt
     3781 acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg attgcatatg
     3841 ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa ggtagaataa
     3901 gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat agatgaacac
     3961 tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta caatctcttc
     4021 ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac cgctctttta
     4081 ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt cgatctctgt
     4141 aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc
     4201 aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat
     4261 ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga agtagaactg
     4321 tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg tcaacacgta
     4381 cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa gtacttgtcc
     4441 acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat ccccagcttc
     4501 cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt tttgtgtttc
     4561 aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac gatcaatacg
     4621 acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg gcaatagtaa
     4681 tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat ccatcaattc
     4741 cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat
     4801 cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa cttccatttc
     4861 taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga aaacaatcag
     4921 gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag gcatcggacc
     4981 gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg taatcatgtt
     5041 tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc
     5101 atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg gagctgcatc
     5161 aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg tagtgatcgc
     5221 agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg
     5281 tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat aatatagatc
     5341 actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa atcaaaaaga
     5401 atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc aatattggtc
     5461 aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt tataaataaa
     5521 aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg aagcaataag
     5581 aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat agacgaaatc
     5641 ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact
     5701 tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt tttacggcgg
     5761 cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc aagcagataa
     5821 cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag atggtgaaaa
     5881 aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg cgtatcttaa
     5941 actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg cttctgaagt
     6001 gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat tttattagat
     6061 aaaaagaggc tgggacagaa gcgtttaact
//
LOCUS       DACCQP010000114         6077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCQP010000114 DACCQP010000000
VERSION     DACCQP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.120"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..124
                     /locus_tag="IVO87_002653"
     CDS             <1..124
                     /locus_tag="IVO87_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7450783.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(210..1304)
                     /locus_tag="IVO87_002654"
     CDS             complement(210..1304)
                     /locus_tag="IVO87_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7450784.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1493..2254
                     /locus_tag="IVO87_002655"
     CDS             1493..2254
                     /locus_tag="IVO87_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7450785.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2369..2809)
                     /gene="fabZ"
                     /locus_tag="IVO87_002656"
     CDS             complement(2369..2809)
                     /gene="fabZ"
                     /locus_tag="IVO87_002656"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7450786.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3029..3784
                     /gene="fabI"
                     /locus_tag="IVO87_002657"
     CDS             3029..3784
                     /gene="fabI"
                     /locus_tag="IVO87_002657"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7450787.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3894..4907)
                     /locus_tag="IVO87_002658"
     CDS             complement(3894..4907)
                     /locus_tag="IVO87_002658"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7450788.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5072..5992
                     /locus_tag="IVO87_002659"
     CDS             5072..5992
                     /locus_tag="IVO87_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7450789.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1864 a         1211 c         1081 g         1921 t
ORIGIN      
        1 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
       61 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      121 ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact ggctatttct
      181 agcggattat ttctgcaata actaattatc tattttttat tttcaattaa tttaaagctt
      241 gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct tcctacaaca
      301 ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc tttgtattga
      361 ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt attatgaggc
      421 gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat atattctgag
      481 cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt ttctgtaata
      541 ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc tgccatttct
      601 ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat caatcctgta
      661 aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac
      721 aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac gaatgccatc
      781 gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa
      841 ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt gtttggcgaa
      901 taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc gattttgata
      961 ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat atctaagcgg
     1021 accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat ctccaactgt
     1081 tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt cgcgtaatca
     1141 actacttctt taaattctga aagaactttt tctttatcat cattgatctg cagcaaactg
     1201 gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt gtccttttca
     1261 aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc atttcctcct
     1321 gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa tggtctacac
     1381 cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg tattttccct
     1441 ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa
     1501 aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc
     1561 tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc
     1621 ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa
     1681 agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat
     1741 caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga
     1801 cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg
     1861 cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga
     1921 gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc
     1981 taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct
     2041 tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc
     2101 actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc
     2161 tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa
     2221 aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag
     2281 atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc
     2341 ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact
     2401 cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc
     2461 ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac
     2521 ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt
     2581 ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca
     2641 ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg
     2701 caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac
     2761 gattaggaat caattccatt acttcagtag cagataatac gtaagacata attaaactcc
     2821 ttctttcagg caattgccat tttcattact ttgactatca aagtatcata ttcctaaata
     2881 tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa
     2941 taattaagaa atcaagaagt tattttagct attattctct atttttttac gactatcaaa
     3001 tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat
     3061 tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca
     3121 aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt
     3181 ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa
     3241 agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc atgcagttgc
     3301 ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag acggctatca
     3361 attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg cacaaccgtt
     3421 tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac gcgcaatccc
     3481 gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc
     3541 agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg caatcaaaac
     3601 attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg aatctcgtac
     3661 gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag
     3721 cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg tccatctttc
     3781 ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa aactttttaa
     3841 agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg aaattattcc
     3901 acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc tgtcaattca
     3961 tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac atgaagattg
     4021 ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca tgcaccagct
     4081 tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg cagtcctagt
     4141 cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat agagggcgat
     4201 tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc acctacttct
     4261 tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc cagttctttt
     4321 gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc tgcaccagtc
     4381 cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt tagtccacct
     4441 ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg agctggatgg
     4501 acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt agcgcctgtt
     4561 ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc atcaacgaac
     4621 atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat ccgtcgacta
     4681 ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc ttttagacct
     4741 tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt caggtaatta
     4801 tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat aaaacgccaa
     4861 ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata cttaccacct
     4921 tcgttatttt ctccacattt tatcattatc catattattt gtcattttat tttcagcttt
     4981 tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg gtacaataca
     5041 gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa ctgccggtac
     5101 gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa atgcgtttca
     5161 gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc aaaaaagttt
     5221 atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa aatatctgat
     5281 tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc gcttaaagac
     5341 tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt ttatctatgc
     5401 aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg gaattgcagg
     5461 tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg gattttttat
     5521 tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact taacgattga
     5581 aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg atggtactgt
     5641 ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg caaatatgca
     5701 agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct atgagatcat
     5761 tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc
     5821 tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg tctgctacgc
     5881 attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg tcaaagaatt
     5941 gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat aatttggaca
     6001 aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaagaac acttttgtcc
     6061 cacgctctaa acacgta
//
LOCUS       DACCQP010000115         6068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCQP010000115 DACCQP010000000
VERSION     DACCQP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.121"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IVO87_002660"
     CDS             832..1566
                     /locus_tag="IVO87_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7450790.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IVO87_002661"
     CDS             1582..2751
                     /locus_tag="IVO87_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7450791.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IVO87_002662"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IVO87_002662"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7450792.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IVO87_002663"
     CDS             3841..4767
                     /locus_tag="IVO87_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7450793.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IVO87_002664"
     CDS             4786..6036
                     /locus_tag="IVO87_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7450794.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACCQP010000116         6029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCQP010000116 DACCQP010000000
VERSION     DACCQP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.122"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(25..1497)
                     /locus_tag="IVO87_002665"
     CDS             complement(25..1497)
                     /locus_tag="IVO87_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7450795.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFIY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVMEGKVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQEKIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(1511..2359)
                     /locus_tag="IVO87_002666"
     CDS             complement(1511..2359)
                     /locus_tag="IVO87_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7450796.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LTSFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2356..3222)
                     /locus_tag="IVO87_002667"
     CDS             complement(2356..3222)
                     /locus_tag="IVO87_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7450797.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(3265..4578)
                     /locus_tag="IVO87_002668"
     CDS             complement(3265..4578)
                     /locus_tag="IVO87_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7450798.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMESGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDGSA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQVIDTSLK"
     gene            complement(4720..5691)
                     /locus_tag="IVO87_002669"
     CDS             complement(4720..5691)
                     /locus_tag="IVO87_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7450799.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGFSPYPNETNNRKAGFYNICEKNKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            complement(5817..6029)
                     /locus_tag="IVO87_002670"
                     /pseudo
     CDS             complement(5817..6029)
                     /locus_tag="IVO87_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1823 a         1304 c         1021 g         1881 t
ORIGIN      
        1 taaatcatca ctgagctctc ccttctatag attaattaag ccgctttcca atgaaaaggt
       61 tacttcactg tctgccagca aaagaaaatc ctgtcctttt tctgtctcat aaaaagttgt
      121 actcatagtc agacgatcat ttagaaaaag ctcaatagct gaagtatcac ggaaaatttc
      181 gatggttaga caattatttt ccaaaggaat cgtcacactg cgctttttca acacttcctt
      241 ttcagctcct gataaatcat acggcatgtg ttccctggaa gcagtaaagc actcccgttc
      301 aacatcatag ttaagcagta atgtatggtg gggtcccttc gcatagtgaa gttctagttt
      361 cgttgcctgt tttaaattga tttccaagcg taaatagtgc tggtcatata ctaagtcctt
      421 cagtaagaat cgattctctt cagtcttcgc ccattcaatc ttttcctgcg gatcaattac
      481 ttgataaatt gatgaaactg gccgttgcaa caattgatta tcagccaccc tcagctctct
      541 aggcaaggtc atggaacctg accaaccatg gcccagatca ttcgtaggta ttttacgctg
      601 ccacatttgc atccaagcag tcattattct ttggcctgct tcgttttcac aggtctgggg
      661 ggcataaaaa tctaaaccgc catcgatttc atgaaaattc tctactagaa atttgcctgt
      721 ttgccaatcg acagtaccaa tgaaagcaac agttgagttc gtattttggt aagcattgcc
      781 ttgtggagtc atttgaatag gagaaagaat caagacttct ttgccatcta gttcaaacaa
      841 gtctgggcat tcccatacaa tgccttgttc tggagttccc tccagcaata cggaataaaa
      901 ctcccaagtt agtaaatctt tggaatggaa catcacaatt tgtccacgag catctgcagt
      961 tttagtagcg actacagtaa aatagtcttc ttttcgctga aacactttgg gatcgcggaa
     1021 atcttcggtc gttgcaacag aagctaagtg atcttctgcg atgattggat ttccggcata
     1081 tttttcaaag tgaatgccat cttctgaaac tgccaaacac tgaacttgtc gaactttccc
     1141 ttccataaca ttgtgtcctg tataaatcaa atacagcttg ccatcctttt ctatagcact
     1201 gccggaaaaa cagccttcct tgtcatagct ttcacctggt gccagtgcta ctggcaaatg
     1261 ctcccagtga attagatcct tgcttttagc atggccccaa tgcattggtc cccagatgct
     1321 gtcgtaagga taatattgat aaaacaagtg atactcctct tgataataaa taaaaccatt
     1381 tggatcattg atccagccaa tcggagccat caaatgaaat ttattccggt attcttgatt
     1441 gactgtttga gcatttttct caataaactc attggccttt aaaagttttt gcaacattca
     1501 ctattcactc ctattttata cccgttccag aaccgccaag tccctgcaga atatattttt
     1561 gtgcaacgat ataaacaata atcagtggaa tcgttgcaat ggttaaaaca gccatcactt
     1621 gattgatata aactggctgt gtagtattga tcgtagtaat tgccacttgc attgaaaatt
     1681 tgctgctgtc agttaagacc attaacggcc aaatataatc attccagctg gagataaagg
     1741 ataagacacc tactgtggct atggctggtt tagacattgg cagcatgatg tgaaagaaaa
     1801 ttttaacaat atttgcacca tctaatttcg ctgattcaat gatttcttct ggaatcgcga
     1861 taaagaaatt tctaaacaaa tagatattaa acgcgctggc catcgctggt aaaatcactg
     1921 ccaagcgagt attcactaag cctagtgaat taacgatggt aaattgcgga attaaaaccg
     1981 tctccatcgg gacaatcagc agagctaaca aagctccaaa gataattttc ttgcctgaaa
     2041 aattaacctt cgcaaaggca aaacccgcca aagagtttac aatgattgac ccacaggcaa
     2101 aggttaaacc ataaaaaata ctgtttaata aataagttcc cacactgaac cgggaaataa
     2161 cttcttgata agttttaaac caattagctg gattcaaact aggtaaaaaa gctttgaagg
     2221 aagttaaatt ttgatagaca tctgcttcag gcttcataga ggaaactacc atccacagca
     2281 ttgggaaaat gaataatgcc gctaaaagga acagcaaaca atattctacg atcattagcg
     2341 gtccaaattt cttagtcatg ctggatcatc ctcctttacc aattttcttt gagcaattgt
     2401 aattaaacca atcatggtgg taaagattaa cgcaatggag ctggagtagc ctaccatacg
     2461 gtctgtaaat ccagtttggt aaatgtaata aaccattgtc atagtagaat tcattggtcc
     2521 accttgagtc attaccattg gctgaatgat tagtttaaag gccgaaatca atgtggtaag
     2581 taaaataaaa atcgccgtgg gctttaataa tggcatggtg atatagcgaa attgctgaaa
     2641 cttagaaaaa ccatcaattt cggctgcctc ataaacgtct tgcggaatgt tttgcattcc
     2701 ccctaaaaac agcagcatct gatatcctgc accttgccat gccgatacga aaacaagcgt
     2761 atacattgct tgtttgggac tagtcaaaaa aggctgcgaa gcaattccaa tctttgcaaa
     2821 tagcgtatta accagacctt cattaggatt taacaagtac aaccataaaa tcgaaataac
     2881 gactaaggac atcacgactg gcgcaaaaaa ggctacttta aaaaagatat ttcctttacg
     2941 atttttgttg ataataagtg ccattcctaa agctgcccct aattggaccg gaataatcca
     3001 aacaacaaat ttagccgtgt tccaaatact ttttacaaaa attggatcag cgaacagtcg
     3061 cttaaaattt tctaagccga taaattgtct agcatctggc gttaacaaat aataatcagt
     3121 aaaggcataa taaattacca tgattacggg tatgatcaga aaaatcgtca gcagcagcaa
     3181 agctggcgct agaaatccgt aacctatcaa attgtctttc attgtcggtt tttttaacac
     3241 ttgattggcc ccctttagaa acttctattt tagtgaagta tcaattacct gttgcatttc
     3301 agcagcgcga ctatctgcta ctttttgtac atctgggttt tcctcgaagt agctaatatc
     3361 ttgaactgct tgttggaatg ctctagatac ttgcggataa gctacaacca ctgggcgggc
     3421 atgtccagaa atttggtttt gcttaattaa tacctgcatt ccttccgaca cttcatcttt
     3481 aattaaatca actgttgaat ttctgatagg tagaacactg ttccctaaac tcataatacg
     3541 actagattct gtactggttg caaacttaat aaaggccgca gaaccatcct ttttctctgt
     3601 tttctcagtc attgctaact gccagctgcc ggttggtgaa actaactccc cagttgcatc
     3661 tgaaacgggg taaggcaaaa taccataatc aatctcttga tagttttcct ctaaatctgc
     3721 cattgtccaa gaaccgctca gcagcatggc atatttgcca gtttcaaatc ctttttcaat
     3781 aggactgatt gtggtgtaac ctttttcaac caattcttga ataaattcca acgcttcaac
     3841 ggatttttta tcgttaaaat agtcttcggc tttggttcca tcagcattaa cgacatcgcc
     3901 gccattcgac catacaaatg gtgtatatgc gtaggtcagc atttcgtctc ccgactccat
     3961 ttgcatatca attgcaggcg ttttgtattt atcagtcagc ttttggcaaa ttgcttgaaa
     4021 ttccgtccaa gtccaaggat cttctaacgt cggcaattga tcttctgtaa ttccagcatc
     4081 tttgaacaac gacttattgt aatagacacc cacactggac tctgagaaac caaacgcata
     4141 cattttgcca tcgtaggttc cttgctgaat aatgctgtct aatacatcat ccatgtccgc
     4201 atctttcaaa taatcatcta atggagcaat gactcctgaa tgagcataag ctgcagtatt
     4261 cggtccatct aacgtaataa catcaggaag ggtattggtt gtaattgcag catttacttt
     4321 atcctcatat ccaccgccgc ttccgctacg aggaatatac tcagtaacag ctttatattc
     4381 accctcgtat tcatcattaa aattgttcac aatctctgcc caagcttttc cttcaggcgt
     4441 ttcatcagaa aactgaaccc aaatcttaat ttctcctgaa tcactgctgc ctgaactttt
     4501 ctccttactg ccacatcctg caagtcctcc agctgttaat gctaaaattg ctgcaattgc
     4561 taacattctc cttttcatcg atgttcctcc tattttttga aacgtttccg ttttattctc
     4621 caattaattg gtaacgtttc caattaattg gttttaagat atcattgaaa ccactttctg
     4681 tcaacctatc tcaaaaaaaa atcgttataa accggttttt tactctttag tcgtttctcc
     4741 agcgtgaaag aaaaccggta ctaccacacg cttaggcggt tcttcattta aaatcatcgc
     4801 catgagtgtt tctactgaaa ccttggccat ttcctctatc ggctgtacaa tcgtcgatag
     4861 taaatagtct ttttgcaacg atagcttgac tccatcaaaa ccaataattt gaaaatcctt
     4921 aggtgcttcc ttccctagtt ttttttgggc ttttatcaca tccaaagcca tgaaatcatt
     4981 gatggtaaaa atgctatcaa tttcaggatt atccgaaaaa aaagcgagca attgttcctc
     5041 caaattgcta attggttcag gcatatccaa taccttcaca gctattttat ttttctcaca
     5101 aatattgtaa aaaccagctt tacgattgtt tgtttcgtta gggtaaggtg aaaaaccccc
     5161 aacatagcac aagtttttac agttacgagt aagtagttct tgagcggcaa tttgcccacc
     5221 taaataatta tctgccgtta cataagaaac ctgttccgaa aaatggcggt ctatactgac
     5281 aaatggtaaa tttgatgata catatttatc aatgtctgaa taggtaatac ctataattcc
     5341 gtctacttta ctctgcttga ccatttggat atattctttt tctttttccg cactgccgtt
     5401 cgcattacac aaaaaaagct tataattttt ttggctgaga gcttcctcta catagtaggc
     5461 aaactcggaa aagaagggat gccaaatcgt aggtaaaata agagctattg tattgctgct
     5521 attcgttttc atacctctgg catattcatc aggctcataa ttaagctcac taattgcagc
     5581 cattactttt tctttggtaa cctctttcac ctttactcca tttattactc tagataccgt
     5641 acccacacca actcctgcta acgcagcaac atccttcata gtagttttca cttcacgaac
     5701 aactcctctc aaaagaattt caggtattat tataccaaaa gaagaaaacg attccaaatt
     5761 agcaggaata tttttatttt aaaatagata ttagacgtga agcttttcgt aaaaaaattt
     5821 tcgtattcag atttaccgga ttgactctgt cgccaatata ggccggcata ctttgtcaat
     5881 tttatttcat cagaaaaacg ttcaattgga ccaatttcag cgagtattcc agcagcctaa
     5941 acaggaccaa tacctgggat actggttaaa cattgatact ccggtagcac cacgactaag
     6001 tcgtcaatgg ctttatctaa gtctttaat
//
LOCUS       DACCQP010000117         5932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCQP010000117 DACCQP010000000
VERSION     DACCQP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.123"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            15..533
                     /locus_tag="IVO87_002671"
     CDS             15..533
                     /locus_tag="IVO87_002671"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7450800.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            661..1344
                     /locus_tag="IVO87_002672"
     CDS             661..1344
                     /locus_tag="IVO87_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7450801.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1653..1726
                     /locus_tag="IVO87_002673"
     tRNA            1653..1726
                     /locus_tag="IVO87_002673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Arg,seq:tct)
     gene            1885..2244
                     /locus_tag="IVO87_002674"
     CDS             1885..2244
                     /locus_tag="IVO87_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7450802.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2367..3998
                     /locus_tag="IVO87_002675"
     CDS             2367..3998
                     /locus_tag="IVO87_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7450803.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4060..4701)
                     /locus_tag="IVO87_002676"
     CDS             complement(4060..4701)
                     /locus_tag="IVO87_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7450804.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4983..5291
                     /gene="rplU"
                     /locus_tag="IVO87_002677"
     CDS             4983..5291
                     /gene="rplU"
                     /locus_tag="IVO87_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7450805.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5318..5662
                     /locus_tag="IVO87_002678"
     CDS             5318..5662
                     /locus_tag="IVO87_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7450806.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5675..>5932
                     /gene="rpmA"
                     /locus_tag="IVO87_002679"
     CDS             5675..>5932
                     /gene="rpmA"
                     /locus_tag="IVO87_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7450807.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         1844 a         1000 c         1268 g         1820 t
ORIGIN      
        1 agaagttgaa aacgatacct gattacaaaa aaataacttt tataagttct ataacgttga
       61 ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc
      121 cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg
      181 tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt
      241 caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt
      301 atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta
      361 tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa acggcatttg
      421 catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga
      481 atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt
      541 aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac
      601 actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt
      661 atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt
      721 acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca
      781 gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt
      841 cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc
      901 acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag
      961 aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt
     1021 caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt
     1081 gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc
     1141 gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt
     1201 ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat
     1261 catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag
     1321 gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa
     1381 tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat
     1441 acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga
     1501 taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc
     1561 caaaataatt cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat
     1621 tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat
     1681 ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta
     1741 gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa
     1801 ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa
     1861 ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt
     1921 gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca
     1981 agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt
     2041 aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt
     2101 tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta
     2161 tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg
     2221 gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact
     2281 ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa
     2341 aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag
     2401 aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag
     2461 ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg
     2521 caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt
     2581 atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa
     2641 agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag
     2701 gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta
     2761 cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact
     2821 cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa
     2881 acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa
     2941 aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc
     3001 tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta
     3061 atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta
     3121 ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa
     3181 tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt
     3241 tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca
     3301 tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct
     3361 ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt
     3421 tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata
     3481 aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt
     3541 taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg
     3601 ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg
     3661 aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag
     3721 aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc
     3781 gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag
     3841 cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac
     3901 tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga
     3961 cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa
     4021 gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga
     4081 agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc
     4141 tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt
     4201 ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt
     4261 atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg
     4321 ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt
     4381 aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt
     4441 cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc
     4501 agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt
     4561 atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc
     4621 ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat
     4681 cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga
     4741 aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct
     4801 tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta
     4861 agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc
     4921 acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata
     4981 gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa
     5041 tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct
     5101 tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag
     5161 gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa
     5221 aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga
     5281 tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg
     5341 aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta
     5401 tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat
     5461 tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct
     5521 ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt
     5581 agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat
     5641 taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt
     5701 atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg
     5761 tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca
     5821 acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt
     5881 tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aa
//
LOCUS       DACCQP010000118         5832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCQP010000118 DACCQP010000000
VERSION     DACCQP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.124"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            165..2330
                     /gene="acm"
                     /locus_tag="IVO87_002680"
     CDS             165..2330
                     /gene="acm"
                     /locus_tag="IVO87_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7450808.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2602..3087)
                     /locus_tag="IVO87_002681"
     CDS             complement(2602..3087)
                     /locus_tag="IVO87_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7450809.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3567..4208
                     /locus_tag="IVO87_002682"
     CDS             3567..4208
                     /locus_tag="IVO87_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7450810.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4227..4862
                     /locus_tag="IVO87_002683"
     CDS             4227..4862
                     /locus_tag="IVO87_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7450811.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4880..5695
                     /locus_tag="IVO87_002684"
     CDS             4880..5695
                     /locus_tag="IVO87_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7450812.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2062 a          923 c         1196 g         1651 t
ORIGIN      
        1 aaaaacagtt ttttggcata tttatttttt ataaatattt gtaaaataac tatcaatttt
       61 ttagttaaaa gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga
      121 tatagtaggt tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa
      181 atattatttt atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg
      241 tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa
      301 cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa
      361 atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg
      421 aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg
      481 tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag
      541 aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata
      601 cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag
      661 cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata
      721 ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag
      781 ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta
      841 atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt
      901 tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa
      961 ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag
     1021 aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata
     1081 aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg
     1141 gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga
     1201 ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac
     1261 agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag
     1321 taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa
     1381 gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta
     1441 tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca
     1501 ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag
     1561 ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata
     1621 tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa
     1681 acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa
     1741 atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc
     1801 aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc
     1861 aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat
     1921 acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta
     1981 cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta
     2041 taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa
     2101 ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac
     2161 aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa
     2221 gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga
     2281 tagtaactat ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata
     2341 atattttttg ttaaatatga ctgaaatcaa aagaataaaa ttccgattta taatcgaaaa
     2401 aagaagcatt tttatatatg cttctttttt cgtagaatca ataaatatgg atttggctaa
     2461 ctatggaaga atactcgtat tagttttcag cgttcatact agttgttaca ttgtttgatt
     2521 ggccaaaaga atttctgata tattttgtgc taaataaaga aaaagacgct tgaattggga
     2581 aataagaaaa ctactctatc attacaaatt ttcataaact ggcagatgtt ctaagtaaac
     2641 aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta gtggtattga tatttggatg
     2701 aaagcctacg gtatcttgac gatcaatagc agcatcgatc acgacttgga tttttttctc
     2761 tttgtcgtgc atcaatgcta atggagtcac tgtacctgct ggaacgccta ggaggtcagg
     2821 aagctgttct tcagaaacaa aagataagcg cttttctttt aattgttcag ctagttgttt
     2881 taaatcggcg tgcttttcat caggcaagat gacaagatag atttgttttc cttttcttga
     2941 tttcaataat agatttttta cttgcggacc aggtagggaa aaagggacat ttttgactga
     3001 cgtaatcgca ggatgatcga ctttttgaaa agagataccc agttttttta gtagttcata
     3061 agcttcttgt tcggttgcac gactcatcgc gcatcaccct ccttttttta ttagtgtatc
     3121 ataaaaaaga gttgacaaat gaaaaataat catgtaaatt acacgtaaag ttaaaacaaa
     3181 tgacatcgaa aagaagagta gccgttaggt gtttttagag agtctctggt tggtggaaag
     3241 aggcagcagt taaccgtgaa acacatcttg gagtcgattt tctgaagcgt atagtaggaa
     3301 aatccgggga gcccgttaca gctgcggtcg ttgctagatg accagataag gctggctttt
     3361 ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg atcttcggat
     3421 caaaggactt tttgttttca tctgaacggc aacgactgtg tgaggttttt tctgcaagga
     3481 aaaaataaac agaggtggaa ccacgtataa tacgtcctct tggcatttgc caagaggatt
     3541 tttttatttt aaaggaggaa aagaagatgg agtatttgat tgaagttatg ccgcaattat
     3601 tagtaggagc actcacaaca ttgaaattat ttttatttac cttgattggc tcattaccat
     3661 tagggattgt tttagcatta ggattgtctt tgcattttaa gccgctacgc tacttgcttc
     3721 aaacatatgt atgggttatg cggggaacac cgctgttgtt gcaactgatt tttatttttt
     3781 atgggttgcc gacgattggg atcgtttttg atcgtttcga agcagcactc attgccttta
     3841 tcttaaacta tgcagcttat tttgcagaga tctttcgtgg agggatacaa tcgattcctc
     3901 aaggtcaata tgaagcagcg caagttttgc aattaagtcc ctttcaaacg gtgacgaaaa
     3961 tcgtcatccc acaagtgaca aagattgttt taccttctgt agggaatgaa gttatcaact
     4021 tggtcaaaga ttcttccttg atctatatat taggtttagg tgacgtaatg cgtgctggga
     4081 aaatcgcgat ggaacgcgac gcgactttac tgcctttgct aggcgtggca atcatctatc
     4141 ttacattgac tggtattcta acaatcataa tgaaacaagt tgaaggaaaa ttaaattatt
     4201 accgctaact gataggagga agaaatatga ttgaattgaa gaaagtgaac aaagcatttg
     4261 gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc agatgggaaa acattagcta
     4321 tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg tatcttggct ggcttagaaa
     4381 gttctgacag cggagaattt tatttagacg gtgtaccgtt caacccattc gagcttaaaa
     4441 tgaaagaaca agttgtagga gtcgtattcc aagacttcca gctatttcca catctgaata
     4501 tttttgaaaa cattacctta gcaccaaaaa tggtattgaa acagacaaaa gaagaataca
     4561 ccaaaaaagc agaagaatta gcggcttcat tagggattag tgatttattg aatatctatc
     4621 cttatcagct ttctggcgga caaaaacagc gggtagcgat tgccagagca ttggctatga
     4681 atccacgggt attggcttat gatgaaccaa caagtgcttt agatcctgaa ttacgccagc
     4741 aagtcgagcg tttgatccta tcattgaaag aagatggggt aacccaaatt attgtcaccc
     4801 atgatttagt atttgcagaa aatgtcggtg atcaattact aaaagtttcc ccaattcatt
     4861 aagaaaggag aagatagaga tgaaaaaaac cagttttcta ttcgctttga tcgcaggtct
     4921 tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca gataattggg cgactatcaa
     4981 cgaagagaaa aaaatcgtta taggtttaga cgatacattt gttcctatgg gatttagaga
     5041 taaagacggg aagctcacag gatttgatat tgatttagcc aaagcagtct ttgaacaata
     5101 tgggattacg gtagactttc agcccattga ctggtcaatg aaggaatttg aactgaacaa
     5161 tggaacgatc gatttgatat ggaacggcta ttccaaaacg ccggcaagag aaaagaaagt
     5221 gcaatttaca aaaccttata tggaaaacga tcaagtgctg atcacaccta aagaatcagg
     5281 aatcacaaac tttggacaaa tgaataataa gagattaggt gcgcaaaatg ggtcatctgg
     5341 ttatgatgta tttacgaaac aaccagaagt attaaaagag atagtcaaaa accaagatgc
     5401 tgttttgtat gatagtttta atgaagcatt gatcgatctg aaaagcagac gggtcgatgg
     5461 tctgctgatg gataaagtat acgcagacta ttatttgaaa caaagaaacg aactagaaga
     5521 cttcaacata acaaaaggtt cttatgagag tgaggatttt gcagttggtg cacgcaagtc
     5581 agatagtaca ttagtgaaaa aaatcaatga agccctagat caactagaga aaacaggtga
     5641 attccaaaaa atatccgata aatggtttgg cgaagatgtt acgccgagaa attaacaaat
     5701 aggggactgt gacaaaagac gtgtcacagt ccctttttcc gaataaacag tgttctcctg
     5761 ccaactcctt gtgactcgga gctgaatggc aggctcacaa cctcttggta aacggtgttc
     5821 aaaagccagt tt
//
LOCUS       DACCQP010000119         5787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCQP010000119 DACCQP010000000
VERSION     DACCQP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.125"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(26..2197)
                     /gene="copB"
                     /locus_tag="IVO87_002685"
     CDS             complement(26..2197)
                     /gene="copB"
                     /locus_tag="IVO87_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7450813.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2217..4403)
                     /locus_tag="IVO87_002686"
     CDS             complement(2217..4403)
                     /locus_tag="IVO87_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7450814.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="IVO87_002687"
     CDS             complement(4403..4612)
                     /gene="copZ"
                     /locus_tag="IVO87_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7450815.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4625..5065)
                     /locus_tag="IVO87_002688"
     CDS             complement(4625..5065)
                     /locus_tag="IVO87_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7450816.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5139..5678)
                     /locus_tag="IVO87_002689"
     CDS             complement(5139..5678)
                     /locus_tag="IVO87_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7450817.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1495 a         1302 c         1009 g         1981 t
ORIGIN      
        1 tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta
       61 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      121 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      181 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      241 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      301 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      361 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      421 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      481 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      541 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      601 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      661 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      721 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      781 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      841 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
      901 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
      961 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1021 tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1081 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1141 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1201 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1261 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1321 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1381 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1441 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1501 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1561 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1621 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1681 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1741 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1801 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1861 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     1921 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     1981 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2041 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2101 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2161 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2221 tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc
     2281 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2341 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2401 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2461 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2521 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2581 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2641 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2701 tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca
     2761 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2821 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     2881 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     2941 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3001 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3061 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3121 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3181 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
     3241 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3301 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3361 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3421 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta
     3481 acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga
     3541 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3601 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3661 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3721 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3781 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3841 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     3901 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     3961 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4021 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4081 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4141 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4201 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4261 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4321 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4381 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4441 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4501 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4561 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4621 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4681 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4741 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4801 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga
     4861 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
     4921 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     4981 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5041 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5101 atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
     5161 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5221 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     5281 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc
     5341 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5401 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5461 tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat
     5521 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     5581 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     5641 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
     5701 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
     5761 tcgtgtcaaa gtcccttgtt tcgaata
//
LOCUS       DACCQP010000120         5572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCQP010000120 DACCQP010000000
VERSION     DACCQP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.126"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(118..432)
                     /locus_tag="IVO87_002690"
     CDS             complement(118..432)
                     /locus_tag="IVO87_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7450818.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IVO87_002691"
     CDS             complement(446..706)
                     /locus_tag="IVO87_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7450819.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IVO87_002692"
     CDS             876..1190
                     /locus_tag="IVO87_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7450820.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IVO87_002693"
     CDS             1192..2847
                     /locus_tag="IVO87_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7450821.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IVO87_002694"
     CDS             2930..5065
                     /locus_tag="IVO87_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7450822.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..>5572
                     /locus_tag="IVO87_002695"
     CDS             5240..>5572
                     /locus_tag="IVO87_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450823.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         1890 a          963 c         1133 g         1586 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aa
//
LOCUS       DACCQP010000121         5500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCQP010000121 DACCQP010000000
VERSION     DACCQP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.127"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            277..1194
                     /gene="rbsK"
                     /locus_tag="IVO87_002696"
     CDS             277..1194
                     /gene="rbsK"
                     /locus_tag="IVO87_002696"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7450824.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            1222..1617
                     /gene="rbsD"
                     /locus_tag="IVO87_002697"
     CDS             1222..1617
                     /gene="rbsD"
                     /locus_tag="IVO87_002697"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7450825.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            1617..2990
                     /locus_tag="IVO87_002698"
     CDS             1617..2990
                     /locus_tag="IVO87_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7450826.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            3347..5491
                     /locus_tag="IVO87_002699"
     CDS             3347..5491
                     /locus_tag="IVO87_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7450827.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         1786 a         1082 c         1092 g         1540 t
ORIGIN      
        1 gcattttttt gatagaatca ttcatatagc atatcgtcct ctcagttgtt tattttgtcg
       61 tgatttaatc atactagaga acgatatgtt ttttaatacc taaaataaaa atggggctga
      121 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaatagct ttcaaggtgt
      181 gctatatttt tatcgaacta ttaaaacgtt ttactaaaac gttttaaaaa ataatttttc
      241 ccatcaaaat aaatgagata gttggaggtt tgtaaaatga acacggttac agtaattgga
      301 agtattaata tggacactac ccttcgttta accaacatgc ccaagccagg cgaaacaatg
      361 catgcgcatg agatttttca cgctggcgga ggtaaaggtg ccaatcaagc agtggcagcc
      421 aaacgttcag gcgcacggac atcttttatt ggaggcgttg gggctgacag cgaaggacaa
      481 cagttattag atctactgac aaaagaaaac atcgatacat ctgggatagc tgaaatccaa
      541 ggcgccacaa ccggacaagc aatgattatg gtagacgcag caggagaaaa tagtatcttg
      601 atccatgcag gtgcaaataa tgctttccat gaacaagaag tcctaaaaaa caagcaactg
      661 attacaaaca gtgactttat tattgctcag tttgaaagta gcttagatgc gacgatccta
      721 gctttttcta ttgcaaaaga cgcaggaaaa acaacgatcc tgaatcctgc accagctcgt
      781 gaaacgatcc ctactgaact acttgaaaaa acagatatca ttataccaaa tgaaacagaa
      841 acagaaatca ttacaggaat cagagtgaca gatcataaca gtttggtcgc agcagccgaa
      901 aaactccatg aattaggtat tggaacagta atcattacac taggcagtgc cggcgctttc
      961 tatcatacag aaaaagaaca cggtatcgtc cctgctttta aagtggatgc cgttgacaca
     1021 acagccgcag gtgatacatt tattggcgca ttaagcagca cacttcagcc agaccttagt
     1081 aacttgaaag aagcgatcct ttatggaaat ctagcctctt ctgttgctgt gcagtcttat
     1141 ggtgcgcaac cttctatccc ttaccgcgaa gctttagaac aatcagcagt ctaaaattaa
     1201 caatgagagg aagaaacaaa catgaaaaaa ggaaaagtaa tcaattcaga tatttcacga
     1261 gtaatcgcac aaatggggca ctttgataaa ttaagtatcg gggatgcagg catgcctgtc
     1321 ccctttggta gtgaaaaaat agatttggct gtctcaaacg gtcttccaag ctttttatct
     1381 gttttagaaa acgttttaga agaacttgag gtacaatcga ttgaattggc ggaagaaata
     1441 aagacaatga acccggaaat attggaaaaa atcaaattat tgttaccaga tacaccgatt
     1501 agctttatcc cacatgaaga aatgaaaaaa gagttgaata actgtcatgc atttattcgt
     1561 acaggcgaaa tgacacctta cgccaatatt atattagtga gtggagtggt cttctaatgg
     1621 aaacgacaat gaatcctgaa cagatggtac tgtccgagca gattcatatg ctgattgaac
     1681 agttgggagg aaaagacaat attgattctg tccttccatg tgcgactcgg cttcgtatca
     1741 aattgaaaaa acagccagta gccaatgcag aaaaattaaa acagcaaatc ccttccatta
     1801 agtatttcat tttactcgat agatatgcag aaataatcat ggatcaggga ttggctcctg
     1861 cttacaagga attaacgcgt caaatcgatc attcagctcc taaagaaaag ctaaatccga
     1921 tcgaaaaact atttggtttg atatcggaaa tctttacgcc gatcattccg ctgtttgccg
     1981 gttctggtat attaaaaggt ctggttgttt tagcgactac gattggctgg cttagtgaaa
     2041 caagcggaac ttatcatatt ttgtctgctg cgtcgaatgc cgtcttttat tttctgccaa
     2101 tctttcttgc tttttctgcc gccaaaacgt ttaaagtgaa tggatatatt gcggctgtca
     2161 ttgctgcagc actgattgaa ccgaatctga cctctttact ttcagacagt ggcaaagcag
     2221 ctactgattt tctggggatt cctgtagtac tgatgcagta tacgtctact gtcatgccgg
     2281 cgattttagg tattttcttt tattcttttg ttgaaaaatt cctcaaaaaa tatattcgcg
     2341 aaaatatgca attagtattt gtacctttac tttcattagt tatcacggtt cctttgacgt
     2401 taatggtatt cgggcctgcg ggtgtttacc ttggcgaagg tttagctgcc gcaattactt
     2461 ggatgatgga tattaacgga ccgatcagcg gcgcaatcat cgctggtggt tggaatattt
     2521 tggtaatatt tggtatccaa tgggctgtaa accctatcat gatcagtaat atctctgctt
     2581 atgggttcga ccgaatcgtt ccgttgacag gagcagccaa ttttggaatg gctggtgcag
     2641 ctttaggtgt atttctacga agtaaacgtt caaaaactcg ttcgatctcc ggttctgctt
     2701 ttgcttcgat tttacttgcg ggagtgaccg aaccaactgt ttacggaatt gcgatcccat
     2761 tgaaaaaacc atttgttgct gcttgcattg gtgcagctgc tggtggagct gtaatgggat
     2821 ttgcccaagt gaaagcgatc gcttttgtat ttggtagtct gaccacattg cctgcattca
     2881 tttccggtac ttttttctgg tacttagcag gactggctgt tagtctagtc gtagcaatga
     2941 ttactactct cgttagcggg tttgatgaag acctaatgtc ttatgaatag ttaatgaaaa
     3001 agactgaagt atgagatgtt tatcacatat ttcagtcttt ttctttttag gatcattatt
     3061 tttataaatt tttgtgatac tttccaaact ttggtataat ttacatatgc ataaccattt
     3121 ttatccccac ttgtttatcc ccaaacaaag gtggtctatg caataaaatg aacgtcaatg
     3181 aaaaacgaat gaatgctcgt tgcttattta caaattgttt tatttagaat acacagacag
     3241 atttatgaaa agaaaaagct tttcgacacg attatctgtt ccaaaaaatg tctaatgaat
     3301 gctgaataag acagaaaatc aatgaaagga aaaggactag tttattatga aaaataaaaa
     3361 aacgatacgt ttgctagctg ctacttggct gattgctccg tctgttttgt cgaccaatac
     3421 cgtatttgcc gtagataatc aaacagctga tcaaacagtc acaacagata cgactacatc
     3481 cagctcagct actagtgaaa caacggattc ttttacagaa gacccaactg gaacaactaa
     3541 ctcaacaaca ggatcgaccg agccaactga aattgatccg gctgatccat cagaaaatga
     3601 aaagccagcc gagacatatt cccttacaat tgctccttct ttgattggta agatccaact
     3661 gactgcttcg acagcggatt cccctgaaca gcaacctttg gatattacgg aaagtggaac
     3721 ggtagaaggt ttattagcgg aaacgaaggt aacttacact attacgccag ctgaaggtga
     3781 acaaattgct tcagtcgctg ttaatggtgc caaagaagta aattatgatg caaagacctt
     3841 tacagtaggt acgaacaatt tatctattac tgcagaattt gaacctgtac cggatacttc
     3901 aacagagaaa ccaactgatc ctagtgaaga agtccctagt acaccagatc cggaaacgcc
     3961 aaacaaacca gatgtgggta ataacaacaa taataatggt tcaaataatt caaacaataa
     4021 taacaataat tcgaacagcg ggaattcttc gaatcaaaca aacccatctg gatcgaataa
     4081 caatggaaat actcagagag aaccaaacca tccaaacaat ggttcatcaa atggcggaac
     4141 gaacaatgct tcatctcgtc caaatacagc aaatcaaagc ggtatagaga atccatcttc
     4201 tgcttcttct gattttgttg taaaatcacc aattgatgct gtattgccta caaatacaac
     4261 gaatgtccaa caagcaatcg ttcgagaagc attcaagcat cttggaaaac catatgtatg
     4321 gggagcaaaa ggtccaaata cttttgactg ttctggtttg acttattacg tatatatgaa
     4381 agcaaccggt cattatatcg gtggctggac aggcgaacag caatatgcag gtacacagat
     4441 tccagtaagt caggcccaac ctggtgatct cgtcttctgg ggaccttctt ctggtgttac
     4501 ccaccacgta ggtatctata ttgggaacgg acaatttatc catgcgcctc aaccaggaga
     4561 taaagttcga gtaacttcta tttcagattt cacgcctgat tttgctgtcc gagtaaactt
     4621 agcaggttta cctgctgctt caggttcgct gacaggcggc tcatctattt tagatggatt
     4681 agatggctcg ttccatttca gtcaaaacca aacaactgat cagttcttga aaaagattgc
     4741 agatgatgca caggaaatcg ggcaaaaaga aggaatctac gcatcagtca tgatggctca
     4801 agctatcctt gaaagtggat ctggaaacag cttattatca agcgaaccaa atcataacct
     4861 ttttggtatc aaaggatcat ataaaggaag cagcgtgacc ttcaatacac ttgaacaaga
     4921 tagttccgga caaagctacc aaatccgcgc acaattccgt aaatatcctt cttacaagga
     4981 aagtttggaa gactatgccg acttgatcaa aaatggactg actggcaatc ctgatttcta
     5041 caaaccaact tggaagtcgg aaacaaaaga ctacaaagaa gcaaccaaat atttggaagg
     5101 acgttatgct acagatcgcc aatattcaca aaaattgaac gcgatcatcg aagcgtacga
     5161 cctgacaaaa tatgatgaac cgaaaaaaga agcagcaaca actgaagaag caaacgctga
     5221 aatcctcaat atgtctaagc gattcgatgt gccaatcaaa tggacaacag atggcgtgcc
     5281 aatcacagga ttagaatttt caacgataaa acgcgtcaac aacgcttctt ccatcttccg
     5341 tgtcgcttca atctgggatt tgtggaacaa ttttacggca agacagatac cagaaacaac
     5401 tactcgtaca gttaaagctg caactgttcc tgtactaacc ttgttaagtt tagacaaatc
     5461 attctggctt gatctgcctt taaagaaata gaataaatta
//
LOCUS       DACCQP010000122         5330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCQP010000122 DACCQP010000000
VERSION     DACCQP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.128"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(229..504)
                     /locus_tag="IVO87_002700"
     CDS             complement(229..504)
                     /locus_tag="IVO87_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7450828.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(476..1429)
                     /locus_tag="IVO87_002701"
     CDS             complement(476..1429)
                     /locus_tag="IVO87_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7450829.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2041..3534
                     /locus_tag="IVO87_002702"
     CDS             2041..3534
                     /locus_tag="IVO87_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7450830.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3669..3965
                     /locus_tag="IVO87_002703"
     CDS             3669..3965
                     /locus_tag="IVO87_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7450831.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3989..4948)
                     /locus_tag="IVO87_002704"
     CDS             complement(3989..4948)
                     /locus_tag="IVO87_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7450832.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5131..>5330
                     /locus_tag="IVO87_002705"
     CDS             5131..>5330
                     /locus_tag="IVO87_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450833.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIA"
BASE COUNT         1735 a          895 c          958 g         1742 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccaaatgt
      121 tgtatacccc caaacaatac acatattttg aaggattagg attaataaag ctaaaaatat
      181 agtaagtata ctaaatatat ttactatatc tagtatactt actatatttt aatcttttga
      241 tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg cgtccattgc
      301 aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc gaatggtttt
      361 tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact ttcgttcccc
      421 taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt tcttcttatc
      481 tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt aggatatgat
      541 tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca ccatgttgtt
      601 catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata gcccaccatc
      661 ttcgatctac ataatcattt aattggatac cattctcacc atacatttct aaccgatccg
      721 agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt gttttttgat
      781 agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt ccaatgattt
      841 gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta ataaaatctt
      901 ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa tctgcagcag
      961 ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag atataatcga
     1021 aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta cgacgatcag
     1081 gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca gccataatat
     1141 ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg attgctgcag
     1201 acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa gtagtatttc
     1261 tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta gaaacaatcg
     1321 ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta agcattacaa
     1381 cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata gttccctcca
     1441 atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg atatatctaa
     1501 tatactccaa attgataaga attcagactt tataaggaat atagtggata tactaaatat
     1561 atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt gtgcgttttg
     1621 cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac agataattca
     1681 ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac ataaaaaaaa
     1741 gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg gttaggacat
     1801 gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat taaaagttga
     1861 tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac tcaacagaag
     1921 aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag aacacatact
     1981 atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac gttacaatcg
     2041 atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata caaatatcga
     2101 aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc tatctttgcg
     2161 taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc ggaagaagct
     2221 attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg ctttggtact
     2281 tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt aagtcaaatc
     2341 aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat tattttagcc
     2401 tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca tggttatcaa
     2461 gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt caaggtgatc
     2521 gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa attgtccgga
     2581 gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa taatattgtt
     2641 tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc gatgaagctt
     2701 gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt tcctgagaaa
     2761 aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa agcaaacatc
     2821 gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc tttagcttat
     2881 tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt caacgaacgg
     2941 ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc ttattctggt
     3001 aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg ggtttcacca
     3061 accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt taaaaagcca
     3121 cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt ggcttatcta
     3181 agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga attggcttca
     3241 caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg agaggagaat
     3301 aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc ttcagtcaaa
     3361 acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta ttcaacaagg
     3421 atcacagagg tatttggact caacgagagc ttagtagaag agacatttag acaattatca
     3481 acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg ttgaaaaata
     3541 aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt tgctcccttt
     3601 gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg tatcatggag
     3661 gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg tttatcattg
     3721 gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag caggggactt
     3781 tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag tttcttcatg
     3841 ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg tgtgtgttag
     3901 agcaggagta tattgagtat aaaatagcta acgagttaag aaaaaggaag aagtatatcc
     3961 agtagtagtt tttatttact cttaattgtc aaattaagtt agacaaatca tcaatactta
     4021 cgtaactcaa aaatacttcc aaaggtgttc gataattcag tgattttcta ggaatattat
     4081 ttcgtttcga tgcgacagat tgaataaaag attcatctac tgaattgaaa tccatagatt
     4141 tcaataaacc atctctacgt aacaatccgt tagaattctc gtttaggcct ctttgagacg
     4201 gcgttcctgg atcagcgaaa taaatggcaa tatcattggc attactgatc tgtttccaat
     4261 ttgaaaattc ctttccacaa tcaaaagtga tggatttgaa tagatttttc ggtacagatt
     4321 caaaccaatg atttaatttg ttttcaatat caatcgcttg tctaccacac ggtttcaatg
     4381 tgatgataac ttttgataat cgctcaacta aggtaattac agcactttta tgtttcaaac
     4441 ctacgatagt gtcaccttca aggtgaccaa actcatttga gaattggcta taatcttttt
     4501 cacgttcatg aatagagcga cggaaagctt gttttccacg tctttcttga tgtccatttg
     4561 gtttacgctt gcctttcatc ggtaagtcag aaggattaaa tagccccttt ttgaacatac
     4621 gataaatggt acgagcagaa caacgaatag gaaacgctgc acgaccaaca atcacatcgg
     4681 gtgtccatcc ttgaacaacc tttctttgaa tatattctga ttgttcctca ggtaaaacaa
     4741 gcgggcgtct accacagttt gatttgtttt tcttatactg ttgatagtac tctaaggctg
     4801 attttccttg cttgaagaac aggtatactt tatggatcgt ttgtcttgaa cgattcaagc
     4861 aatgcgcagt tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt
     4921 catccgttgt aagatgggta taggtcattt gtactcactc cttataattc tttggacgga
     4981 actattttga gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc
     5041 gtacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     5101 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     5161 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     5221 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     5281 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc
//
LOCUS       DACCQP010000123         5029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCQP010000123 DACCQP010000000
VERSION     DACCQP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.129"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            23..982
                     /locus_tag="IVO87_002706"
     CDS             23..982
                     /locus_tag="IVO87_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7450834.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1846..2058
                     /locus_tag="IVO87_002707"
     CDS             1846..2058
                     /locus_tag="IVO87_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450835.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(2336..4135)
                     /locus_tag="IVO87_002708"
     CDS             complement(2336..4135)
                     /locus_tag="IVO87_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7450836.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(4259..4855)
                     /locus_tag="IVO87_002709"
     CDS             complement(4259..4855)
                     /locus_tag="IVO87_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7450837.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1790 a          878 c          874 g         1487 t
ORIGIN      
        1 gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat
       61 aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg
      121 ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga
      181 atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc
      241 cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat
      301 tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa
      361 aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca
      421 tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta
      481 tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca
      541 taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc
      601 gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc
      661 gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca
      721 gatcagtaat gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag
      781 aggcctaaac gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga
      841 tttcaattca gtagatgaat tttttattca atctgccgca tctaaacgaa ataatattcc
      901 tagaaaatca ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga
      961 tgatctgtct aacttaattt gacaattaag attatattaa atattataca gataataacg
     1021 gaatattatc atcgaaacag ctaacaaaat tgtcataaaa aacgagatgg ttgatattag
     1081 aacttgtgtt tttgtttagg tctagagata aagagagaca atttccaaat atatgtaatg
     1141 aatacaaaaa gacgtttgtc gcggattacc gttacaaacg tcttttattg attttccgag
     1201 atctcatcag tcgccatttg gaatggttca ctccttatct agattcgttt aatgctcgga
     1261 ctgcttgttc gattgtaata ccaaacgcaa cgttattttc gttttgtgca tcaatcgcca
     1321 taaaacggtt agaagttgta tcaaaagcta cgcggtattg catatttgtt tttgtaaacg
     1381 tcataaaatt catctccttt gtaatgtaat ttagttcttg aaagtttcct ttaaactttc
     1441 gaacttatac caagtttagc atcgtacgtt ttttgatgca attaaaaagc atttatcatt
     1501 ttccttattt attttttctt aaatggttta gctattttct tttaatccga taagtagccc
     1561 aatcatcaag gttacaaata ctattgcttg aatataaatc atatgctcac ctctttataa
     1621 ataacttagc aatataaaga aaaaaacgca cgaacaacat aggaatataa acaaacgttt
     1681 ttgtcacaac gtctaagatc gtgttaaaaa atagttatct aaaaagcaga aagatactca
     1741 aaataaaaaa taaaaaaatg gataaaaacg ttttcaaata caaggtaatt tgttattata
     1801 aaataaagaa aaaacaaaat aaaaataaag aaaagaggga atgaaatggt aaaagcagaa
     1861 gtaggaagat cttatatatg caaaccgatc ggagtatctc atgaactaat cggctgtatt
     1921 gaacgtacat atatcaacac ggctgtcgtg tatgttgaaa gttatgacca acgagactat
     1981 caacttatca atgaaagcaa gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct
     2041 gctgaattgg tttcttaaac agtatttttg agaattcgca agaactccct tataatataa
     2101 ataaagacag aagggagcga gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa
     2161 ttattcttgc agtgatctct gcaattcctg ctattttagc tattgttcgt gcgatgaagg
     2221 aatcaagaga aagttaagca cacattatga gcgtcaaaca aaaaacgcat atacctgcgg
     2281 tggcaggtta tgcgtttttt gttgtataaa gacttttttc attaacgatt tttgattagc
     2341 cgactcccaa aacaaaattt agcagtttat ctgaataagt gatttcttca tctgtttgtt
     2401 tcacactttc atttagtagt cccatctcat cggttatgat accatccaca ccataaaaca
     2461 tcatgcgact catagcatct gaatcgttga ttgtccatac atatacttct tttccgtctt
     2521 ggtgggcaga attaatgaag ttacggttga tcgtactata ttccattgtc agaaaatcag
     2581 caggcgtcac agggggccca acaatattga aaggtaagat gtagcccaca taaaagtttg
     2641 gttctgcttc ttttaaatct gaaacggttt ggtacgtcaa actttgcaaa atatgatggt
     2701 tctctaagat aactttgcgg tatttatgga caaatcgttc gacaaggtca tccgaatctt
     2761 ttttagaggt cttgatctca ataagtaatt tttggtttag cttatgtgct ttttccaaat
     2821 aagaatcaaa agaacaaaca ggggcttgca ctccgttttc agtaactgta agatttgtca
     2881 attctttcaa tgtaagatcc tgtggtgttc catttacccc tgttaagtga cgaagatttg
     2941 catcatgcat cactacgaaa tgaccatctt ttgtttcctg aacatccatc tctacataat
     3001 caggatgata gtctctgctt gttttttcaa gtgcttctaa agtattttgc gccccatttt
     3061 gggtactcac acctctgtga gagacagtca ctggttttgt ctgaacagct gaagtcagat
     3121 aatccatatt gtaaagacag acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag
     3181 tgtttttcag cgcagagaaa cgcaagttgg gggcttgtgg gataaaccac ttagggatct
     3241 caggcaagaa gccttcgtca tccatgaaat caacaatgat ataaaaaatc cctacggtag
     3301 acatcacgat attgaataat aaaatccctt gtaaaagcgt catagcaaac acagcgctga
     3361 tcaatgcata gtctgggaaa aattgttcaa ccatactttg tgccaaaata acaacgatat
     3421 atcctaaaga agaaaggagt aaaatcgtac cgccaatcac aataaattgg cccgtgatgg
     3481 ccaaaagacg cgatttggtc aatgaccagc tttcccgaat ggctgctcga aaaggacgat
     3541 ctcgtagaat catttccggt aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa
     3601 tataaacgag taaaaaggaa ctgacgatga tccaacgatt agcaaaaata aaatccataa
     3661 tgaaagcggg gatttttatt tttgacagta gatcagaatt aaaacttaat ccgctgatcg
     3721 gtagaatcaa taaaaaataa gcaaggaaaa acaaaaaagt aatgggtctc actttcttca
     3781 gctgaagtat cgtcaagtgt aagagttgct tcaaagagat tggttctttc tttttaataa
     3841 aaaaaacact catcaaaaga aaagtaaatt cgaaataaac taacagtaaa atcgtcatca
     3901 aaactacgat taacgagagt agaaccccag gatgttgaga aaaaattgaa ggaatattgt
     3961 cataagacaa atagtcaatt gcccctcggt gtaaaataaa acgggtcgta ctgctcaata
     4021 agggtaaaca aataaataaa ataaagccat gcatcagcaa gacatcacgg aaataagctt
     4081 gtgttccttt taaaaagtcc caagcattac ctaagctacg tttcaaataa ttcatcctat
     4141 cacctgctct tcacattgta gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt
     4201 gcagaccctt tcccagattt aacattgtca atcaatgact aattttacaa aataaatatt
     4261 atccaagaag accacttaga tattgacctc cataaatcaa ggccatacta tataagaaaa
     4321 tcgacagtgg tttagcaatc aaaatgatta gtgtatatcg tttcactgac atgtttgtca
     4381 atccagccat caaacataac gcatcgtcag gagcaactgg aagaaaaata gctagagcaa
     4441 acagcttctc aaaacggttt tgattatcca gccagccaat atatttgtta tatgtcttat
     4501 cgctaatcat ggaaagtatg aatggctttc cataacgacg ccctagcaga aaaatgataa
     4561 tcgaaccaat gcagattcca atatagttat agataaatcc agcataaggg ccaaatatca
     4621 ggacaccagc tgcagtactg atccctccag gaatgattgg aataactact tgcaggattt
     4681 ggatgaagac aaaaataatc ggtcctaaaa tgatggaatc acctactaat cctcttaacg
     4741 cattcaaatc tttgaaaaca cctaaattaa taaaataaat cgtcagcgcg attgataggc
     4801 ctaatccgat aatggatatg aaatttatca gttttcgaga ggcagcaata ttcattattc
     4861 atcaactcct tagaggttct ttgttaacta tactttacta gaaaagcaac taactgcgcc
     4921 attttttgat atgacagaaa acaaacaatt ttgtaagttc atcataagta taaaagattg
     4981 atctcaaaaa aacatccaac taaagactta tttaataact tgttttttt
//
LOCUS       DACCQP010000124         5022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCQP010000124 DACCQP010000000
VERSION     DACCQP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.130"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            283..522
                     /locus_tag="IVO87_002710"
     CDS             283..522
                     /locus_tag="IVO87_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450838.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IVO87_002711"
     CDS             complement(884..1144)
                     /locus_tag="IVO87_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450839.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IVO87_002712"
     CDS             complement(1329..1826)
                     /locus_tag="IVO87_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7450840.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IVO87_002713"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IVO87_002713"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7450841.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IVO87_002714"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IVO87_002714"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7450842.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..>5022)
                     /locus_tag="IVO87_002715"
     CDS             complement(4548..>5022)
                     /locus_tag="IVO87_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450843.1"
                     /translation="IVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVS
                     VIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFY
                     YTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1527 a         1042 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tg
//
LOCUS       DACCQP010000125         4998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCQP010000125 DACCQP010000000
VERSION     DACCQP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.131"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            192..290
                     /locus_tag="IVO87_002716"
                     /pseudo
     CDS             192..290
                     /locus_tag="IVO87_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IVO87_002717"
     CDS             complement(513..743)
                     /locus_tag="IVO87_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7450844.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="IVO87_002718"
     CDS             complement(973..1791)
                     /locus_tag="IVO87_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7450845.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1952..2641
                     /locus_tag="IVO87_002719"
     CDS             1952..2641
                     /locus_tag="IVO87_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7450846.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2655..4157
                     /locus_tag="IVO87_002720"
     CDS             2655..4157
                     /locus_tag="IVO87_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7450847.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4170..4646
                     /locus_tag="IVO87_002721"
     CDS             4170..4646
                     /locus_tag="IVO87_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7450848.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4743..4853)
                     /locus_tag="IVO87_002722"
                     /pseudo
     CDS             complement(4743..4853)
                     /locus_tag="IVO87_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1687 a          723 c          884 g         1704 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1921 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1981 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     2041 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     2101 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     2161 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     2221 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     2281 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2341 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2401 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2461 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2521 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2581 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2641 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2701 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2761 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2821 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2881 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2941 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     3001 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     3061 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     3121 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     3181 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     3241 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3301 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3361 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3421 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3481 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3541 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3601 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3661 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3721 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3781 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3841 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3901 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3961 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     4021 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     4081 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     4141 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     4201 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     4261 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4321 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4381 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4441 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4501 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4561 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4621 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4681 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4741 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4801 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4861 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4921 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4981 tatataccaa atatagta
//
LOCUS       DACCQP010000126         4939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCQP010000126 DACCQP010000000
VERSION     DACCQP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.132"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IVO87_002723"
     CDS             complement(207..1169)
                     /locus_tag="IVO87_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7450849.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVO87_002724"
     CDS             complement(1171..2610)
                     /locus_tag="IVO87_002724"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7450850.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELFLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVO87_002725"
     CDS             2795..4741
                     /locus_tag="IVO87_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7450851.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGVNQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1596 a          828 c          803 g         1712 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaaataac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagtaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttgtctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtctattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatgtatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaat
//
LOCUS       DACCQP010000127         4924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCQP010000127 DACCQP010000000
VERSION     DACCQP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.133"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..916
                     /locus_tag="IVO87_002726"
     CDS             <1..916
                     /locus_tag="IVO87_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7450852.1"
                     /translation="LSIRVQISLGKYQDQLLKLEKKLESGLYCELVEKELKDSYVEYT
                     LLYDMIANRIGIDEVVAENGTLRLMKNQVWAYDSLPHMLIAGGTGGGKTYFLLTIIEA
                     LLKSDAELFILDPKNADLADLGTVMPHVYSQKEEISACVEDFYERMMARSKAMKEMSN
                     YKTGENYAYLGLPPNFLIFDEYVAYMEMLTTKESAVILNKLKQIVMLGRQSGFFLILA
                     CQRPDAKYLGDGIRDQFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGS
                     VISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            994..1668
                     /locus_tag="IVO87_002727"
     CDS             994..1668
                     /locus_tag="IVO87_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7450853.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1901..2335
                     /gene="dcm"
                     /locus_tag="IVO87_002728"
     CDS             1901..2335
                     /gene="dcm"
                     /locus_tag="IVO87_002728"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7450854.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            2336..3043
                     /locus_tag="IVO87_002729"
     CDS             2336..3043
                     /locus_tag="IVO87_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7450855.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            3033..3323
                     /locus_tag="IVO87_002730"
     CDS             3033..3323
                     /locus_tag="IVO87_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450856.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            3582..4766
                     /locus_tag="IVO87_002731"
     CDS             3582..4766
                     /locus_tag="IVO87_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7450857.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4781..4900
                     /locus_tag="IVO87_002732"
     CDS             4781..4900
                     /locus_tag="IVO87_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7450858.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1582 a          809 c         1143 g         1390 t
ORIGIN      
        1 actgtctatt cgcgttcaaa tttcacttgg gaaataccaa gatcagcttt taaaattgga
       61 aaagaagtta gaaagcggac tgtattgtga gttagtagaa aaggaactca aagactccta
      121 tgtggagtat actttactgt atgatatgat cgccaatcga attgggatag acgaagtagt
      181 ggcagaaaat gggactctgc gattgatgaa aaatcaagta tgggcttatg attctttacc
      241 ccatatgtta atcgctggtg gcacgggtgg agggaagacc tatttcctcc tcaccattat
      301 cgaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa atgcagattt
      361 agctgattta ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa tttctgcttg
      421 tgtggaagat ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag aaatgtctaa
      481 ctacaaaaca ggagagaatt atgcctatct tggacttcca cccaactttt taatctttga
      541 tgaatacgtg gcttatatgg agatgttaac gacgaaagaa agtgcggtga ttttgaataa
      601 gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc tttctgattt tggcttgcca
      661 aaggccggat gcgaaatatt taggagacgg aatccgcgat caattcaact ttcgcgtggc
      721 tttgggtcgt atgagtgaac tcggatattc catgatgttt ggggaggtcg aaaaaaactt
      781 ctttatgaag cacatcaaag gtcgaggtta tgtggataca ggtggcagtg tgattagtga
      841 gttttatacg ccacttgtac caaaagggta tgatttctta gattcaatta gtaaattaga
      901 aaagatgaat gattgagtga tcttcaatac tgactttata cttcaagtaa cgtataatac
      961 ttttatatag taggaagaaa ggggataatt aatatggaaa gtaatgattc ttatttagag
     1021 tatgcaaaag caaataagga taagtttatt tcttatatta tcaaagggaa atcccccgaa
     1081 gttggacgag acgcagtttt tatggcaggg agtcctgggg ctggaaaatc agaggttgcc
     1141 attggattag ctgagaatta tgataaccat gtgattattg atgcggatta ctttcgcacc
     1201 cagtttcctg attataatgg aaaaaactct agtctctttc aaaaagcatc ttcttggttg
     1261 gtagagcagg catttagata tgtcttagat catggctatt cttttattct ggatgcaact
     1321 tttgcaatat taagtgctga aaaaaatatc tcgcgggcgt tgaaaaaaca ttttcgagtg
     1381 acgattttct atgtttatca agaccctaaa atcgcttgga aatttacaca agaacgtgaa
     1441 attgcagagg gacgtcatgt gccgaaaaag gcatttatta acgctttttt caaatcaaga
     1501 gagaacattg caaaagtaaa gagtaaacat ccagatgtat tgcttcatat cataataaaa
     1561 gattatacga ataatatttc cgaagttcat tatgctacgg ataatctaga attattttta
     1621 ccactagggt atagtaggaa agatttggag gttcaattac atgactgaga aagaacaagt
     1681 aacgaaaatt gtaaagaaat acaataagag cattgcggat ttatcggaaa atgctactgc
     1741 aaaagagttc aaaacggtga ttaaatatgt agccgatcag gccaatgaaa aacaacggaa
     1801 attagttgga ttaaataaaa aatagtagga caaattaata atgtatgagc caagagcaaa
     1861 atgttcttgg cttttttctt tgcaatgaat gaggtaatgt atgagatttt tagatttatt
     1921 cgccggtatt ggtggctttc gtttaggtat ggaacaggcg agccatcatt gtatcggttt
     1981 ttgtgaaatt gataaatttg ctagacgtag ttataaagca attcatgata caagtaagga
     2041 ggtggaaatg catgacatca caagtgtatc agatgagttt attcaatccc tcggaccagt
     2101 ggacatcctt tgcggcggat ttccgtgcca agctttttca attgcgggaa agcggcaaag
     2161 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcacca ctcttctccg
     2221 acctaagctt ttattccttg agaacgtccg aggattgctc aatcacgaag gaggggctac
     2281 gttcgagacg atcctccgaa cgctggatgg attggggtat gacgtggagt ggtaggtgct
     2341 taactcaaag gcctacattc cccaaagccg ggagcggata ttccttatcg gacattctcg
     2401 agacgcatgt accgaacaag tatttcctat cattggaccg tcttcaacat ctgatcaaaa
     2461 catcagaaat ttggtaaata tcaatccttc caatcgagga atgggtggac aagtctatgg
     2521 ttcagatggc gtagcgccaa ccttaacagg tgatgaagga atcaaaatcg cactgccagc
     2581 aagggatgga atctctgtag caggactatt accgggaaag tttgaacaag gtaatcgagt
     2641 ttatgaagtt actggaaccg cccccacctt gtcaacgaaa caaggtggta caaaaatcat
     2701 gattcgaaaa aatgaaacag aatatcaaga ggtgaagcca gaagacagtg tgaatctggc
     2761 ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa cagagtgttc ataccttact
     2821 aacaggagac caacaagcga tcgtcacgga tcagtatcaa attcggaagc taacacctag
     2881 agagtgttgg cgacttcagg gctttcccga ttgggctttt gatcgagatt ctcaagtcaa
     2941 ttcagatagt cagttgtata agcaagccgg aaactctgta acagttccgg tcatttttga
     3001 tattgccaga agattaaaag agttggatgt acatggttta tgatgagttg attggagaaa
     3061 tttattgggt gattggaaag attcagtcag acccagaatt agaagaacaa ttgcgacggt
     3121 taaaatttga tattcgcaag aatggtgtca aagtcgctgg cgatccatat ttaatgaatg
     3181 aggagacgga tgctcgaatt gaaataaatc aagtaattgc agaatttgag cggattgcgg
     3241 accaagcaaa agaaccagat attcgtcaat acttgttaga aatgaaagca gagttggaag
     3301 taaatggtat tacagatgag taacctaaac caacacaact gactgttagg cgctgaccgc
     3361 ggaggattcg cgaagcagaa atcctccgtg gtgctgtgcc tgttcatgga cggcgttagc
     3421 cgaccatgat gtctcacccc cgtatctaac aggggggtac aaaaacacaa aaaataagta
     3481 agcaaccatt aagaatgctt gttgtatcaa agatagtaag taatttgtat aggtgttaac
     3541 tcatttgaaa aagttaacac cttgaatttg gaggtgagaa cttggcacaa cggaatttag
     3601 attatcggct attaaaagac cgacgcaacg aatacggcgt ttcacaaaat aaattggcta
     3661 cggcttgtgg acttagtcgg ccgtacttaa atcagataga aaatggtggc gtaaccgcat
     3721 ctataaaaac catgaggaaa atttttgacc aactggaaag ctttaatccc gatttgcctt
     3781 tgacgttact gtttgactat gtaaggattc gctttcccac aacggatgca cgaaaaatta
     3841 ttcaagaaat tctccatttg aaatttgagt atatgctcca tgaagattat gccttttact
     3901 cctatcaaga acagtatgtc atgggtgata ttgtggtgat gttgtctcat gaagaagata
     3961 aaggtgtgct tttagaatta aaggggcgag gttgccgaca atttgaaact tttttactcg
     4021 ctcaaaagcg tagctggtac gactttttcg aggattgtct aaaggccggt ggcgtgatga
     4081 aacgcttgga cttggcaatc aatgatcgag tggggctatt ggatatacct gagctaacga
     4141 aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac ctttaaaagt tatcgttccg
     4201 gggaactttt aaaggctgat gaaaaggacg gcatgggcaa taccttatat atcggcagtc
     4261 tcaagagtga ggtttatttc tgtttgtatg aaaaagacta tgaacaatat atcaagttag
     4321 ggattccatt agaccaaact gagacgaaaa atcgttttga gattcgactg aaaaatgacc
     4381 gtgcttttca tgcgattcaa gatttgttaa aaggtcgtag cattgagagc accacgtttt
     4441 ccattatcaa tcgttacttg cgatttgcgg ataaagtaga aggtaaaagg cgaaccaact
     4501 ggcccttgaa tgaacaatgg gggcgtttta ttggacggaa tcggaaagag attcaattaa
     4561 cttccgagcc aaaaccgtat accattgaac gaaccttgaa ttggctaggc cgacaagtcg
     4621 cccctacgtg gaaaatggct aaggagttgg atcgattgaa tcggacaacc tatatccagg
     4681 atatggtaca gaatgcacgc ttatcagacc gacataaaaa gattttggag caacaaagta
     4741 tggcaattga aaatttgatt atatgaaaag aggaatcgtc atgggaattg taaaagatat
     4801 ttttttggta tttggtggca gtatgcttgg tgttactgtg atgtgcttga tgaatgcaag
     4861 tgcagcagcg gatcgagcaa tggaaaagaa aggaaagtga caagatgaat ttcggacaaa
     4921 attt
//
LOCUS       DACCQP010000128         4809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCQP010000128 DACCQP010000000
VERSION     DACCQP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.134"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IVO87_002733"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IVO87_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7450859.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="IVO87_002734"
     CDS             complement(3252..3770)
                     /locus_tag="IVO87_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7450860.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DACCQP010000129         4792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCQP010000129 DACCQP010000000
VERSION     DACCQP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.135"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..221
                     /locus_tag="IVO87_002735"
     CDS             <1..221
                     /locus_tag="IVO87_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7450861.1"
                     /translation="NTGLTFSIENGADEVAALELETKVMQDEFGKFYHEAIVAELEED
                     LTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            241..603
                     /locus_tag="IVO87_002736"
     CDS             241..603
                     /locus_tag="IVO87_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450862.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            606..788
                     /locus_tag="IVO87_002737"
     CDS             606..788
                     /locus_tag="IVO87_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450863.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            805..4224
                     /locus_tag="IVO87_002738"
     CDS             805..4224
                     /locus_tag="IVO87_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7450864.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4275..>4792
                     /locus_tag="IVO87_002739"
     CDS             4275..>4792
                     /locus_tag="IVO87_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7450865.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFI"
BASE COUNT         1680 a          734 c         1022 g         1356 t
ORIGIN      
        1 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
       61 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
      121 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
      181 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
      241 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
      301 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
      361 aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc aaaggctgtg
      421 tatattgctt accgccaagc gaacatgact gattatattt cattcgatga atttatcgat
      481 aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat gttcaaacaa
      541 gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa gcttcaaaag
      601 taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg ttgaccgacg
      661 ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac attttgcaaa
      721 tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac caatcccaga
      781 aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga agtaacattc
      841 atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga attaactaaa
      901 aatagagcag aattaaaact agaacaagct caattacaac agactggttc tgaatcagac
      961 aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag tcaaaaagtt
     1021 gaagtaacta gccaacgttt agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa
     1081 gttcagaaac ttgagagaga actgattaac caacaaacag cgcaacaacg tttgtcaaac
     1141 gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca gacgtacgag
     1201 tctacaatgc aacagttgga tagtgaacaa aaaaatgttc aagctagtgc ttctctgatt
     1261 gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga atccgagaaa
     1321 ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcagagaa aacgatagat
     1381 atcctgagac gacagttaga agctacacag tctgagtttg gcgctacatc cacagaagca
     1441 atgcagatgg aggcgaagct taatgatgct gaacgtgaat ttgaagagtt aggacaagct
     1501 gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga tatgaataat
     1561 ctaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga attagggaaa
     1621 caagcagtgg actctgctaa tagtgtaggt agttcccaga gtaaaataca agctaatttt
     1681 ggtttgacta aacaagaggc tgaagaatta acgaatgtag ccagagacat ttattataaa
     1741 ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa gcgtaattta
     1801 ggcgatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt cctagaaaac
     1861 accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat ggtcaatttc
     1921 ggcttgagcg ctcaagatgc aatggattta atggtttcgg gtactcaaaa cggtttagat
     1981 aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag tcaaatggga
     2041 tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg tgcttataac
     2101 cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga tggtcgattt
     2161 gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga gtggaaaaat
     2221 ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag caatatggat
     2281 ggtcaatacg accaattaaa taaagcttct acaatttggt ctgcacttgg cgaagataac
     2341 gcgatgaaag ttgtccaatc tttgactgat gttaaccata catttgatga tgttagtgga
     2401 tctgcacaaa aaatgaatga agattctact actccgttac aagaattaaa cggaaaaata
     2461 gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc actcgaacca
     2521 gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca accagtacag
     2581 gattatgccg tagcgattgg cggattgact gctgcattta ctttattaat gccaataata
     2641 gttggcttca tggctctagg tggtcctact acattaataa taggagcagt tattactgtt
     2701 attgctggag ttatagcaat tataaaaaac tggggcgcaa ttactgactg gtttaaggga
     2761 atatggagta aattcactga ttggttgggt ggtacttggg aaagtataaa agaaggtgcc
     2821 tcatcagttt gggatggagt taaagaaacc tggtctggat ttgtagattg ggttcaagat
     2881 atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt tgaaggagct
     2941 tccaacatct ggcaaggagt ccaagagact tggcaagcat tcgttgattg ggtttcaaat
     3001 atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt aggaattgtt
     3061 caaataccat ggaccttaat aacgtcattg attcaagccg gtattaatat tatcgtgggt
     3121 atttttgatg tagctggaca gttattaggc gcagcttggc aagctgtttg gacacctatt
     3181 tctgatttcc ttaaaaacac ttgggatact atgacacaat gggtaagcat cgcttggaat
     3241 ggaattgtaa ctacattcca tactatattt gatccagtag tggcatggtg gaatggtata
     3301 tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc aacagcttct
     3361 agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc tgctacagta
     3421 attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa ttcaatcagc
     3481 tctacagcat caaatatctg gaatagcgtg actagtagta taagcaatgc tataaacgca
     3541 gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggatcag cggaatttgg
     3601 aacggtatca aaaacactgc tttgaatctt tggaatggaa ttacaagcac tattagctct
     3661 aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac aggtattgtt
     3721 tccgacatat tcaataatgt taaaagtaca attgctaaca tttgggaagg tatcaaaaag
     3781 actgttagcg ctccaattga ttggatcaga gataaaatca gtagtatctt tgataatttg
     3841 aatatttcta taccacatat tccgttacca cattttaaat tgagcggaga attcaatcca
     3901 ttgaagggga aaatcccaac gttgggtgtt gattggtatg cgaaaggtag tgtgtttaat
     3961 tctccgaata ttatcggtgt cggcgaagca ggacctgaag cagttttacc tttgaaaaga
     4021 tctgtgctgc aagaaattgg tgatcgtatc ttgagtagca catcagtttc atctagggca
     4081 caaacgattc aacctgtgaa taactacgaa ttcaatttca caattgatgg taacgcagat
     4141 gaggttacta tgaagcaaac aactcaacaa atcattgata gcattacaaa agttcaaaat
     4201 gataatgctt cggcatggcg ttaaacagga gagtatttct cctgtttttt tagtattaaa
     4261 aaggatgtga aaaaatgact gattgtatac attctataat cgatggattt cctgattatt
     4321 tgcataaatt ggctttagct gaaagaccaa ccataccttc tccaaaaaga cagagagttg
     4381 aaacttctgt tttaggaagg ttaggtggct tagtacaaga ttactcgttt gaagacatgt
     4441 cgtttacatt gcactataac tatttagagg atgtggaaga ccatcaagcg ttcaagcaat
     4501 cgttttatat catgcgtcat tggttaaatt atgcaaagaa attagaattc tctgatgatc
     4561 ccaacgtcta ttatgttatc cagactatcg atattgggga tgcagaaaac gatattgttg
     4621 aatggggaga gttcgatgta aatattactg cgaaaccatt cgcaagagtt caagaagatg
     4681 tacctataac cgtagataaa ccacagtcat ttaacttgct gaataatagt ttagaagaaa
     4741 gttttccaaa gattatcatc actccttcag ctacttcatg ccagttcatc tt
//
LOCUS       DACCQP010000130         4727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCQP010000130 DACCQP010000000
VERSION     DACCQP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.136"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..427
                     /locus_tag="IVO87_002740"
     CDS             <1..427
                     /locus_tag="IVO87_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7450866.1"
                     /translation="DMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQS
                     LIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATD
                     ELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            439..1443
                     /gene="neuB"
                     /locus_tag="IVO87_002741"
     CDS             439..1443
                     /gene="neuB"
                     /locus_tag="IVO87_002741"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7450867.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1440..2588
                     /gene="neuC"
                     /locus_tag="IVO87_002742"
     CDS             1440..2588
                     /gene="neuC"
                     /locus_tag="IVO87_002742"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7450868.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2561..3238
                     /locus_tag="IVO87_002743"
     CDS             2561..3238
                     /locus_tag="IVO87_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7450869.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3228..4370
                     /locus_tag="IVO87_002744"
     CDS             3228..4370
                     /locus_tag="IVO87_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7450870.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4383..>4727
                     /locus_tag="IVO87_002745"
     CDS             4383..>4727
                     /locus_tag="IVO87_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7450871.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYL"
BASE COUNT         1752 a          609 c          924 g         1442 t
ORIGIN      
        1 tgatatgaca cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc
       61 tatgaattca aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac
      121 tttagctcaa tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga
      181 aataaaagtt gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga
      241 cgaaattgaa cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc
      301 tactgacgaa ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc
      361 aacagaatta atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac
      421 ttggtaagga gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac
      481 cataatggaa gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct
      541 aacgcggtta aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg
      601 gcaaaatatc agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa
      661 ttagaattat ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt
      721 gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta
      781 caaggattta aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat
      841 atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag
      901 gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc aaattatccg
      961 gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata
     1021 cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg
     1081 ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg tccagatcat
     1141 aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa
     1201 atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta
     1261 tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa
     1321 tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt
     1381 ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa
     1441 tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg
     1501 ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat
     1561 cccataaata tggatttact aaagaattga tcttagctga cggatttaga attagtggag
     1621 aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag
     1681 agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag
     1741 gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag
     1801 cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa
     1861 cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa
     1921 tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa
     1981 ataatagatt acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga
     2041 aaaaatacgt tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac
     2101 aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac
     2161 ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg
     2221 atattattta tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca
     2281 aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag
     2341 taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa
     2401 atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa
     2461 aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa
     2521 aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag
     2581 cttattaaaa tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt
     2641 cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg atgataagca
     2701 aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga
     2761 tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa
     2821 gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc
     2881 tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat
     2941 taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca
     3001 tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt
     3061 acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat
     3121 tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc
     3181 cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa
     3241 ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta
     3301 aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa
     3361 aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc
     3421 ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt
     3481 ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag
     3541 aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa
     3601 gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt
     3661 atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg
     3721 aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct
     3781 attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg
     3841 ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt
     3901 attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg
     3961 gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc
     4021 gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca
     4081 tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag
     4141 tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac
     4201 cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta
     4261 gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag
     4321 atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt
     4381 ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc
     4441 aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg
     4501 aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt
     4561 ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag
     4621 aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg
     4681 atgaaataat cgaagtagct aaaaagaaag atagattact ttatcta
//
LOCUS       DACCQP010000131         4695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCQP010000131 DACCQP010000000
VERSION     DACCQP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.137"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..111
                     /locus_tag="IVO87_002746"
     CDS             <1..111
                     /locus_tag="IVO87_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7450872.1"
                     /translation="GRYFRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            188..517
                     /locus_tag="IVO87_002747"
     CDS             188..517
                     /locus_tag="IVO87_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7450873.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            507..794
                     /locus_tag="IVO87_002748"
     CDS             507..794
                     /locus_tag="IVO87_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7450874.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1080..1937
                     /locus_tag="IVO87_002749"
     CDS             1080..1937
                     /locus_tag="IVO87_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7450875.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1938..2486
                     /locus_tag="IVO87_002750"
     CDS             1938..2486
                     /locus_tag="IVO87_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450876.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2746..3474
                     /locus_tag="IVO87_002751"
     CDS             2746..3474
                     /locus_tag="IVO87_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7450877.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3504..4631
                     /locus_tag="IVO87_002752"
     CDS             3504..4631
                     /locus_tag="IVO87_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAP7450878.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFSKKNLDI"
     gene            4595..4695
                     /locus_tag="IVO87_002753"
                     /pseudo
     CDS             4595..4695
                     /locus_tag="IVO87_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1699 a          627 c          972 g         1397 t
ORIGIN      
        1 ggtaggtatt ttagaagatt gaatagacaa caccgggttg tttataaggt agatgaagat
       61 gaacacgtgg ttgaaattta ttcagcaagg acgcattacg agtcacagta attacttgta
      121 aatacattat ctcgtggtat aatgtattta ctaagtaatt acatactgtt agaaatgagg
      181 gatttaaatg attacaaaaa caagaaaaca aggcaattca atcatgttga cagtacctaa
      241 agattttaat gttcctaatg gagtcgaagt tgaagcaaag ttggtagaga atggtattct
      301 ttatgaattt gttgaaccac aaaaagaatt ttatgatttt agcgaagata ttttgtctga
      361 tataattgca gaaggctacg ataaagatga gattcttgta gaatttaaga atagaaaaaa
      421 caaaatgcat tcttccttta gagatattgc tgaagataca cttactaatt ctaaagtaat
      481 gactaaagag gagttggcaa cagaaattgg cttatgaaat cgttccttca aaacatgtca
      541 ttaagtattt aaaaaagcta aaagagaagc cattgaaaga aaaatttttg aatttaattt
      601 atgatgaaat tgcgattgat ccatataagg gtgaacaaaa aactggggat ttatcaggta
      661 tttggtctag tgggttcaaa tatgcgggca caacatatag agtagcttac gagattatag
      721 aaaacaaggt tattccagta ttgttatgtg gaacgcatga aaacttttat gaacagttga
      781 aaaaattaat ctagtgattt gttgtcgttc ttaagaagga cggcttcttt tttatcgaaa
      841 atctgattcg tctacatgac taaagtcgcg agtattctct gcttctcgat aaattaaata
      901 acgtcattac tttgaggaaa gctataaatc aatatatttc agcttttcct tatttatgta
      961 ctacctttta cttattaatt ataaggtagt acatatatat cttttttttg gtagtaacta
     1021 aaaataaata tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg
     1081 tggtagcgaa gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg
     1141 cgcaaatgtt agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact
     1201 tggatatgca aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg
     1261 aagaaggagg cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga
     1321 taaacaatct tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc
     1381 cagcagacat gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat
     1441 tatcccaatt caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca
     1501 tctatttaga tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg
     1561 actgggcaat cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc
     1621 ttgagtatat ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta
     1681 ttcctttcat gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag
     1741 agatttatgg ggaacatttg attaaaaatg ttgtactcaa aaatgcacgc ttaaaacgat
     1801 acgacggatc aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc
     1861 gcaaaagcca cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt
     1921 atgcggaggt ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag
     1981 gactcgccaa aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat
     2041 ctgaaaataa gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg
     2101 aaagacagag tagaggaaga acaaaaaaag ttggcgcacc aatcagaaag tttgataagg
     2161 tttattccac caagcatcct ttaaaactgt cgactttgtt aaatgcgact tccagagttt
     2221 tagtagaaaa gtatgagact aatatgacaa gagatgaatt gcttagaaaa gctttagatg
     2281 agtatattaa acagaatctt acgcaagaag ataagcaaga tttgtttaat gacgtggtga
     2341 aagaactaga cttgttcaga gaaaaatatc caattgttcc agaattagat gatcgtggag
     2401 aaataataag aactaccagt cagataaaac aagataccga agactcactg cgcaaagggt
     2461 ggggaatgaa taataaagtt agataataat caaagaagaa ctaagtaact acctttaaac
     2521 ggtagttact tagttcttct ttgatttgcg tgaaatagag gggatttatt tttctggtta
     2581 cgtgattaaa aaatccaaaa aatgagcata ggaacggtta tgattttagg gtgtgtttta
     2641 atgagatatt gtgatttcat taacaagtgt atgataatct gttgttgagt tagtaaataa
     2701 gtaatagagc taactctaaa ataaaactat taggaggaga tgtaggtgaa aaagtttaaa
     2761 aagaaaatat atttaggatt ggttgttggg gtgagtatgt ttgggttcat ggctcatact
     2821 aaacaagtaa ctgcagatac taaccaaaaa gaaaggatta tgagccaatt agatgactct
     2881 caaatagagt atgtacccaa tgagctaatt gtcagtttaa aggctacaca aagaatttca
     2941 acagagaaaa ataaaccgca aaatttattg ggtgtaaaag ttatggtgga aaaagatttg
     3001 actcttagga caaatgaaag cgattataat aagcgagatc aagtattatt agtgaaattg
     3061 aacgaagaaa ttagtgtgga agaggcagta agaacattta atgcttctga tatggtcaat
     3121 tatgcagagc caaactatgt ggttgaacta gagccgttga atgatgttga ggagccaaat
     3181 acaactgatt ctttaggaaa tcgatcaatt attcttaacg atcctaattg gagtaaacaa
     3241 gctaatttaa gagagattag catgccatcc gcttgggaag aaaccaaggg ttctaaggat
     3301 gtgaaggtag cggtcattga ttcaggaatt gattatcaac atgaagatct ttctggaaat
     3361 gtagatgaaa gtttgagata cgattttttt gacgatgata cagatcctat ggatactatg
     3421 ggacatggac acatattgcc gggattattg gtgctcaaac aaacaacgaa ttaggtgttg
     3481 ctggaataaa ttgggatgtt acgatggtac ctatacgtgt atttggtagt agtggaggta
     3541 ccagtttggc aatcttaact gaagctatta attatgcatc ccaaaaaggg tttccaatta
     3601 tcaattttag tataggtagt gttgtttata gcagtacaga agaagcaatt aaaaactatc
     3661 ctggattatt cgtagcagca gctggaaata atgggataga tatgactgat aaaccttttt
     3721 acccagcttc tttggatata cctaatatta ttactgtagg aaattctagt ggttatgaac
     3781 gaagttctga ttcaaattat agtaaaacag gtgttgattt attcgctaga gggtttcgtg
     3841 tttttagtac atttctagga agtaaataca cgtatatgtc aggtacctcg atggcaacac
     3901 ctcatgtcac tggtagtgct gcattagcgt tgtcactaga tcctgatcta tctactgatg
     3961 aattaaaaaa aatcatttta aatagtgttg attcatttta tgaactatct gatttatgtc
     4021 ttacaggggg aagactaaat acgaataatg tcgtacgaca agtaacgggt aaacttactg
     4081 catctaatat cacattaaga ttaaatagtg aatggtcaga tgaaattgct aaagagatgt
     4141 gtcacgttca ctggattgac cagtcaggaa atgatttgac ggatcaagtg gttgttctaa
     4201 aaaacgaagt ggatacatct aactttggcg attacgaagt agtatttgca tcacctgatt
     4261 taacagatca aatctcagtt acagtgaccg tacctcgatt acgtaaactt acgctaggaa
     4321 aagaaacagc aaccattgat attggttccg catgggatag tgtcatagcc attgatactt
     4381 ttcaagtgaa agctatagat gattttggga atgatgttac aaatcaagta caggttacta
     4441 ttaatgctga tatagataca attaattcaa ttcaagcagt gattaagttt gaagttccag
     4501 aattaggctt agcccaaatt ggtagactga atattactgc aaaaaatagt acaatcaatg
     4561 gattaaatga agttagcatc gagcacgtaa aaaagattat gtcgttttcg aagaagaatt
     4621 tggacattta gagggtgata cgattgtcgg catccaccat aaaagtgtcg tcatcacact
     4681 cgttgagcga ctttc
//
LOCUS       DACCQP010000132         4634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCQP010000132 DACCQP010000000
VERSION     DACCQP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.138"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IVO87_002754"
     CDS             174..518
                     /locus_tag="IVO87_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7450879.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IVO87_002755"
     CDS             519..938
                     /locus_tag="IVO87_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7450880.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IVO87_002756"
     CDS             complement(1030..1380)
                     /locus_tag="IVO87_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450881.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="IVO87_002757"
     CDS             complement(1346..2677)
                     /locus_tag="IVO87_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7450882.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="IVO87_002758"
     CDS             2933..3499
                     /locus_tag="IVO87_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7450883.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="IVO87_002759"
     tRNA            3644..3731
                     /locus_tag="IVO87_002759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4634
                     /locus_tag="IVO87_002760"
     CDS             4065..>4634
                     /locus_tag="IVO87_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7450884.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgga
//
LOCUS       DACCQP010000133         4491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCQP010000133 DACCQP010000000
VERSION     DACCQP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.139"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..392)
                     /locus_tag="IVO87_002761"
     CDS             complement(<1..392)
                     /locus_tag="IVO87_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7450885.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIA"
     gene            complement(405..1622)
                     /locus_tag="IVO87_002762"
     CDS             complement(405..1622)
                     /locus_tag="IVO87_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7450886.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(1612..3207)
                     /locus_tag="IVO87_002763"
     CDS             complement(1612..3207)
                     /locus_tag="IVO87_002763"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7450887.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLSSTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            3333..4118
                     /locus_tag="IVO87_002764"
     CDS             3333..4118
                     /locus_tag="IVO87_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP7450888.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVVNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKPTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1395 a          872 c          726 g         1498 t
ORIGIN      
        1 tttgcaattt gatggaccac ttcataagta gagattcctc caccaatgtc ttgattggaa
       61 taaaggataa gtgagacaga acccaattgc gaatcttccc gttcaatcgt gatccgagcc
      121 atcccatttt caccttttaa ccatttcgca gcatcaaatg gggttttcgt tcgcagcagt
      181 aattccgttt tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt cagttctgct
      241 tgattatttg agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc tgattttaga
      301 tccggacgat acgtccactg attatgccct tctccaagta cacgaatcaa ctgttcttcg
      361 acttctgctt cctcacggtg actaattttc attcttcttc cctcctagac aaacatcttt
      421 tgtaagaagc tttttttcgt ttcttttagt aaatctaact tacgttgatg aagagcgatc
      481 gtatcatcta gctgtttgaa gaaagtaccg attttttgtt gttcatttat atccggtgga
      541 aactgaaatg tcctttgttc tataccacct ttagtcacat gtaccattgt cgttccgtta
      601 gttgattctt taattttctc tttatcagtc aatagccaag tatatagata ctgtttgtcc
      661 aattccttag ataaaaattt aagtttccaa atatgataat ggtagattac acgttcttca
      721 ttccacattt caggaccgaa gcttgttgcc cataaatata agagatctcc tttattagca
      781 tacttgtctt cctctaattc taagtttgaa taataccatc tatcattagt attaaaatta
      841 cctactctta aaaccctata ttttccagaa tcaagtaatt cttgttgttt aaaagctcta
      901 ccattcaaaa aaactaccac ttcccccaac ttccgctctt cccaatctcc cgtaaatcct
      961 ggaaaacgaa cttctggcac ttttgctccg tttttaggga acattttttg taagaatcct
     1021 tttttcgttt ccttcagcaa atctaattcg tgctggtgaa gcatgatggt gtgatcaagt
     1081 tgtttgaaaa atgaaccgat cttgatctgt tcgtcataag ttggaagtga gataggtgtc
     1141 tttttaccac tatcaattgt ataggttccg acggttccaa tgcctgcaac gtcacggata
     1201 tacttctgaa ttttgttatt tatgaacgag tagtaaaaga agtttccgtc aattttatgc
     1261 tcattcttga accaaatgat gtttccatcc ttaaagtaaa ttgggttatc atcagtgatt
     1321 aataagggga cacctattga tccgacacca gttacaagca agtctccttg agaaacttta
     1381 cctgaatttt tcgaatattc attatatttt tcttctgaaa tgtaaagata atcggatggt
     1441 tcctcatttt ttgcagctga gacaatatca cgagcacgta aaaatctaat acctgagtta
     1501 gtccaatctg attgatgaat tcgcttcaca gaagtaacat ccatcatatc tcctaactta
     1561 cgctcttccc aatcttccgt aaaaccagga aaccggattt ctggtcgtgt atcattgctc
     1621 atgtttgaac acctccagcg caccttgtaa ccacgcttca ttttcaggtg ttgtttgcag
     1681 ggaagagata gcttctaaaa gttctttttc tagcacttgc tgttcttgtc gtatcttctt
     1741 gatgtcattt ccgacatgaa ccatatcaat tggttcttct tcttcgaaag tatctacata
     1801 gcgtgggata tttaagttat agtcattttc tttgatttca tcaaaggttg ccaaatgagc
     1861 gtatttctca acatcttttc tttcagcgta ggtttcgagg attttttgaa tgttttcttc
     1921 cgatagttta ttttgatttt tccctttgac aaattcacga ctagcatcaa tgaataatac
     1981 atctcgagtt tgacgattct tcttcaatac aatcaccgtc gttggaatcg atgtcccaaa
     2041 gaacaagttc gcaggcatac cgatcacagc ataaatactg ccgtcttcta ataatttttg
     2101 acgaatgacc ccttctgcag cgccacggaa taaaactccg tgtggcaaga cgatagccat
     2161 ggtacccgtt tccttcaaat gatagaaacc atgaagaaga aacgcaaaat ctgctttgga
     2221 ttttggcgct aattttccat agcgattaaa ccgagaatca tctaagaagg tcgtatctgc
     2281 tgaccaatta gccgaataag gtgggttcat cactactgca tcaaatgtgt aaggctcatc
     2341 tgttggccag tccttattca acgtatcccc attgcggaga ttcatttctt cggcgtcgac
     2401 accatgtaaa atcaagttca tttttgctag attgtatgtc gttgtattta attcttgccc
     2461 atgatattta acgttatctg ggtgagttaa gtagttacgt acgtttaaca tcaatgaacc
     2521 tgaacccatc gttggatcaa aaacactaaa gaatcgtcgt tctttttgat ccaacgtcac
     2581 gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa aattctccgg ctttcttccc
     2641 tgcttctgaa gcaaattgac tgatcaaaaa ttcatacgca tccccgatca cgtccccatc
     2701 gtgacctaaa acttccacgt cattcaattt cttaatgact tcggtaatcg tcacgtttcg
     2761 ttgttgctca tctgttccta gtttttttga ttggagatcc acatcatcaa ataacccatt
     2821 aaattggtta taagtagaag acaattgcac aaaggcttta tttaaatcat tcaattggaa
     2881 cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggtg agatcgaata
     2941 ccccaaaata tcaacgattg tggcaatcag gtcttctttt gattcttcat ctgaaagtaa
     3001 ttcacgatac aacattgttt gtttgcttac tgtgtcatat tcctctaagg attcatctgc
     3061 taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagtcccaa
     3121 taaataattt ttatattctg acgcatccat tttactacgg agattatctg ccgcactaaa
     3181 taatttttga tttaattctg cacccaaaaa gatacactcc tatctcttat ttaaagttgc
     3241 tatataccct cttattttag catactccct tttagaaaaa gcaaaaaaaa aaagaaaaaa
     3301 tcttattctt gataacaagc tacgtgacgt atatgcctaa caaaaataga acttttgaat
     3361 caatcttaaa tgaaggttta accacagaac cggcatccat tatttttgat gaagttatcg
     3421 atgacgttga agatagtaag tatcataaaa atttaaaaga acttttttat tttgcactca
     3481 acaatggacc aagtttgttc actggtatat cagtacctga tagtgataat ccattaatta
     3541 tggcaaaaat ttacttaaca aaatggtgta ataaatacat cagggatagg aataacccag
     3601 cattaaaaaa acctcttaaa acatttggcg aaaaagatgc tgctttaact actagagtag
     3661 cagcaaatgc taatattgat gatcaaaaag tagtgaatga ctacttgcgt ggacattttt
     3721 tatatatgag cgctgagaac atgaacggaa gtattttaga ggaatattta gccgaagtgt
     3781 tagaacctga aggttggatt tggtgcgccg gctctgtata tcgagctgtt gatttctgtt
     3841 atttaggaac ctcaccaatt cttttacaag tgaaaaataa atacaataca gaaagcagtt
     3901 cgagttcagc tataagagta ggaacaacaa ttagaaaatg gaatcgttta aataagccga
     3961 caaaaatttc tggtttggat agccctatcc ccaattggaa agcattaata gaaatgactg
     4021 aagctagtaa ggaactggca gctaagttaa ctgaaaatag ttatttagct tatattaatg
     4081 aaaaatccac acgagaatta tggacactcg atgactaaca atatccttat tagtatctat
     4141 ccacgataca tggaaaaaat tataaacaaa caaaaaaact ttgaatttag aaatttctat
     4201 cttacctcag atccttataa tatcttttgg atttacgaaa caatccccac caaagcttta
     4261 aagtataaat tgattgtaaa aaatccaatt actaaactta agaaaaatca acactacttt
     4321 ttaggagacg atgagtttga acaattagtt taaaagggaa aatatgctta cgaaattatc
     4381 cacctagaag aaatactttt accaataagt ctgtctaatt taaaaaattt aggagttact
     4441 gcaccacaag gatatgcata tataaaaaaa tatcctagtc ttgtcgatat t
//
LOCUS       DACCQP010000134         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCQP010000134 DACCQP010000000
VERSION     DACCQP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.140"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IVO87_002765"
     CDS             complement(83..1051)
                     /locus_tag="IVO87_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7450889.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IVO87_002766"
     CDS             complement(1498..2904)
                     /locus_tag="IVO87_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7450890.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IVO87_002767"
     CDS             complement(3126..3488)
                     /locus_tag="IVO87_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450891.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3492..3800)
                     /locus_tag="IVO87_002768"
     CDS             complement(3492..3800)
                     /locus_tag="IVO87_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450892.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IVO87_002769"
     CDS             complement(3809..4228)
                     /locus_tag="IVO87_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450893.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IVO87_002770"
     CDS             complement(4221..4361)
                     /locus_tag="IVO87_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450894.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1233 a          995 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tataaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcta tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 agtgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaactcac gtcattaaac attgacgtaa atttactctc actcatctga
     3181 ctttttttac tcggtatcac tccgagtaag ccgtcagatt cattgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACCQP010000135         4271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCQP010000135 DACCQP010000000
VERSION     DACCQP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.141"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(26..529)
                     /locus_tag="IVO87_002771"
     CDS             complement(26..529)
                     /locus_tag="IVO87_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7450895.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(652..1416)
                     /locus_tag="IVO87_002772"
     CDS             complement(652..1416)
                     /locus_tag="IVO87_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7450896.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1524..1799
                     /locus_tag="IVO87_002773"
     CDS             1524..1799
                     /locus_tag="IVO87_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7450897.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2237..3187
                     /gene="yidC"
                     /locus_tag="IVO87_002774"
     CDS             2237..3187
                     /gene="yidC"
                     /locus_tag="IVO87_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7450898.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(3366..3566)
                     /locus_tag="IVO87_002775"
     CDS             complement(3366..3566)
                     /locus_tag="IVO87_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450899.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1258 a          909 c          750 g         1354 t
ORIGIN      
        1 tatggtctat tattggtttt atctattaaa tcgaggattc tttcccaaag taacgttgcc
       61 atctcttacg catacttgct gacataggct gagcggcttc tttgactgcg cagtatttgt
      121 ctacgaatgt tacgatatat ccttctttgt acttaggcgg tgcaatcgtt gcgccccaca
      181 tgtgcttcaa aatgatatct cgctcaagat ccgacaattc ggtgatcttt tctgcattct
      241 ttacagcaat tcttggatgg atatacgcat gtgtgccttc atcaaatttc gtcgtacgcc
      301 aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta gcattgccac
      361 cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc tccaaacgag
      421 tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt tctaaaagat
      481 cttctacata agacatatat tcttcgtctt cacgccattt ttctgtcata attatcgatc
      541 tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca ccagtgttcg
      601 gattattcta gtcgccttgg ataaaatagc caaagcttaa tagttcctta atcataaaga
      661 aaaatgataa agaacaatcc ctgccgcaac cccaacattc aacgattccg catttccttt
      721 catcgggata tatacgttct gatcagtcaa agacaagatt tcctgactta ccccctgacc
      781 ttcgttcccc atgattattg cgaagttatc tgatttttct actttgtcta atgcgtgtgc
      841 ttcttcattt aattctgtgc catacactgt aaaccctttg tctttcatta aagaaatcac
      901 ttctgtcaaa tctttttgca acacaggcaa atgataattg cttccctgca tgctgcgcag
      961 cacctttgta ctataaatat ctgccgttcc tttgcctaat aaaacaccag ccatatcaaa
     1021 tgcatctgcc gtacggatca tcgtccctac atttccagga tcttgtacat tatctaacaa
     1081 cagccatcct cctgaaaaat ctggcgtgcc ttcagccgtt aacgccatca ctgctaagat
     1141 tccttgtggt gtaggtagat ccgatacagc atccatgacc tcatccgaaa ctagaaaagt
     1201 agaaatctct ttgtttgcgg ccagccagtc tccccattct ttcatgcctc gctgtgtcac
     1261 aaaaagttct tttatttctg catcagacga cgcagcttct tcaatcagat ggaacccttc
     1321 caaaagatat tcttcctttt cttcccgata tttcttttta tgtagttttt ttaattcttt
     1381 gattcttgga ttttttgctg attggatctc tttcacattc ctcacctccc catgattata
     1441 tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat taatttataa
     1501 aataggaaag aaaggtgacg aacatgcgca aagttaaaat gaacgtccaa ggacgcgtcc
     1561 aaggcgtagg atttcgctac atgaccaaga tggtcgctga tcaactaggc gtgactggaa
     1621 cggtaaaaaa cgaagatgac ggctcagtca cgatcgaagc aatcggcaat gacgacatca
     1681 tccagaaatt tattgaagaa gtcaagaaat ctccgagccc aagtggacga gtgcagtatg
     1741 tggatgtcca agaagacccg atgattgagg aacgaaagaa atttgatgtg gtgggataag
     1801 gttgtgaaaa agctggtctg catcaagcat taaggacagg ttgtgaaaaa tagctctcca
     1861 tatttttcaa caggctggac ttaatgccgt agatgcaagc ttttgaacac cgtcgataag
     1921 gttattcttg aggcgctttg cctcaaaaat taaggacagc agtgaaaaag ctgatctatc
     1981 acttgcagtg agttcatttg actccgcttt ttcgggcaga aaaaaagttt ttagtttatt
     2041 aaagtaagaa agaaacactt ggaatcattc tactttttcc tagtatttct ttcttgattt
     2101 tttgtctttt tttcaaaatt tttcgctacg taaaagaacg aatatttata tgaaatttct
     2161 atgaagttga ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt aaaaattata
     2221 gaagaggaag tattctatga ataaatggaa aaactggtta ttgggatccg gacttgtcag
     2281 tatcctgctc tttttatctg gctgtgtgcg gatggattca aacggaaacc ctgacacttc
     2341 gggaatcgtt tatcgagtac tcgtccatcc tatgggccaa gcgattacat atctagttga
     2401 aaatttcaat tggtcttatg gctgggcagt tattgcaatg actgtgatcg tccgtatcat
     2461 catcttgcca ctaggtatta gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca
     2521 agcgttaaaa ccacaagttg aagcagccca acaaaaattg aaagctgcaa caacacgtga
     2581 agaacaaatg gcagcacaag cagaaatgca gcaagtatac cgcgaaaacg gattgagtat
     2641 gaccggcgga atcggctgtc tgcctctatt gatccaaatg ccgatcttct ctgccttgta
     2701 ctttacggca cgttatacag aaggtatccg ggaatcatct ttctacggca tcgatttagg
     2761 ctcaccaagt atggtacttg ttgccattgc aggggtcgct tatctgcttc aaggatacat
     2821 ctcaacgatc ggtattcctg aagaacagaa aaagacgatg cgtacgatgc tgatcgtctc
     2881 tcctgctatg atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac tttactgggt
     2941 agtcggtggt gtgttcagct gtctgcaaac attcatcaca aacgttatga tgaaaccacg
     3001 tttgaaagca caagtggcag aagagttgaa acaaaaccca ccaaaacaag tcgtcactcc
     3061 aagaattgat gtgacagaat cagctaaacc agctgacagc aaacaagcaa ccaaacaaat
     3121 caagcaaccc aaaaacagta caaacaataa aggccgcaac gcaggaaagc agcagaagcg
     3181 gaagtagata gtaaataaga gtgtaataaa aattcattaa cagttttagc aattaaaaat
     3241 atcgaccaag atttttgaaa aagaaatctg gtcgatattt ttatttaagt gaatgtttaa
     3301 tttgaatcat cgattgatcg ctatctaatt gcagcagcat ctttttatga agaaacaact
     3361 ttttcttatc tatcaacgta gacaggtgtt gctgtttctt ctaatagtgt acttaattcc
     3421 ttaatatttt gttttccttc ttctaacatc caactactcg ctttttcttc gccttcggta
     3481 acaaaaggcg ccactccctt cgcccatgtt gctcgcccac ataagacacc attctccata
     3541 aaggacaacg cggagatacc acccatctaa ataacatcca taaacctttc tagtagaaga
     3601 atagtgatag tatccatccc tttttctact gggaatacaa ttacacgatt caaagcttga
     3661 tacacaacat ttcccttctt ataaaccgct attgtaaccg aattgttttg aatcaccttt
     3721 ttgggataga aactggctcc tttccgcaac ttcttaaatg ttggtttctt ttatactttc
     3781 tttgtagcaa ctacaaattt cttcaaatct ctaaatgttt gcataacaac cgcctttctg
     3841 acttttactc aggatggtat ttaaaaatca gcaaaataag gcttctctcc atctttttta
     3901 tttcctagct gctttaaaga cactcttgct gttttttaat ctctatcaag ggggctctga
     3961 atctattttg atgaacttat cttattaatt tctcaaatga gctgtcagac acaactgtat
     4021 tttttcaatc tcataccgga caactatgcc agcttaaaaa aatttcctct ggcaaaactt
     4081 ctttattgaa aaatgacaat gaaaaaagac cattcttttt gttgaatgct cattgtgtaa
     4141 acatatttcc tgcgactgct cttcgaacca ttctcagtgc aatccctaat ttatctatta
     4201 aatcttcttt tagttcctca tctttcatat tcaaaatttc ctttgtatgt tacacaagtt
     4261 tgttctctaa a
//
LOCUS       DACCQP010000136         4052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCQP010000136 DACCQP010000000
VERSION     DACCQP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.142"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(96..1562)
                     /locus_tag="IVO87_002776"
     CDS             complement(96..1562)
                     /locus_tag="IVO87_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7450900.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(1853..3937)
                     /gene="cadA"
                     /locus_tag="IVO87_002777"
     CDS             complement(1853..3937)
                     /gene="cadA"
                     /locus_tag="IVO87_002777"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7450901.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1222 a          865 c          643 g         1322 t
ORIGIN      
        1 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
       61 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
      121 tcagtgattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
      181 atcaagacga ttggtgtgat gatattcgta aataattgga accaccatgc tttaggatca
      241 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
      301 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
      361 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
      421 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
      481 ccaaaaatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
      541 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
      601 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
      661 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
      721 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttaggaat attatcacta
      781 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
      841 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
      901 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
      961 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
     1021 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
     1081 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
     1141 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
     1201 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
     1261 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
     1321 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
     1381 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
     1441 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
     1501 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
     1561 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
     1621 acaaacttac aagacagtgt tattttatgt gtgtattctt ttatttaata cacatcgttt
     1681 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
     1741 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
     1801 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
     1861 cctctttttt tacatgcaag cttcgcatgg cattcaaaac agctaatact gtcaccccaa
     1921 catctgcaaa tactgcatct cccattgatg caaaaccaaa agcacctaag gcaagtacca
     1981 aaatttttat cccaatagca aagacaatgt tttgtttgac gattttcaat gttttctttg
     2041 ctaaatggat tgcttctgca atcttcgatg gttgatcatt catgatgact acatctgctg
     2101 cttcaattgc agcatcgctt cccaatccac ccattgcgat tcctacatct gctcttgcta
     2161 gcaccggtgc atcgttgatt ccatctccga caaatgcggt ctttttattg tttctttgta
     2221 agatttcctc taaccgctgc actttatctt caggaagcaa ttcgctatat atcttgtcca
     2281 cgcctacttg cttcccgata tgatcagcaa tttttttcga gtcgcccgtc aacatgactg
     2341 tgttcttcac accagcctgt tttaattgaa ccaatgcgtc tacggcatct tctttcaagg
     2401 tatctgcaat aacaagataa ccactgtaac tttgatcgat cgctagataa agcaatgtac
     2461 cgatttcttg acttaaagga aaagagattt caaatctttc catcaatttg tgattacctg
     2521 ctaatacttg tttgccatca acttcagcta agatcccttc acctgcaaat tcctggatat
     2581 tagttgttgg taaaagcgga cctgcgtagc cttctaaaac agattgagca atcggatgtg
     2641 tagaaaactg ttctgcactc gcaatatatt gcatgaattt ctttggatcg atttttgtat
     2701 cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc ggttttatcg aataccactg
     2761 tctcggtttg cgcaagcaac tccaagtaat ttgaaccttt tactaataca ccgattttac
     2821 ttgcgcctcc gataccgcca aagaaactaa gtggcacgga aatcactaat gcacatggac
     2881 atgagatcac taaaaatgtc aatgctcgat agatccattc agaaaacgcc gctccagaaa
     2941 caacaagtgg aggtaaaaca gctaataaaa cggccaatcc tacaacgact ggtgtgtaat
     3001 agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt tttactgctg gcattttcta
     3061 ctaattctaa taatttgctg atagtagaat tttcataaga tttttctact tgaactaaaa
     3121 atggtttatt tttattgata aatccgctta atactgtttc accaacatat acagatttag
     3181 ggactgattc tcctgtaagt gctgacgtat cgactaatga ttctccttcg atgatcgttc
     3241 catctaacgg tacacgttca ccaggcttca caagtacaaa ctgaccgatc tgcacgtctt
     3301 cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg agctgtttct ggacggatcg
     3361 ccatcaattc gcgaatcgat tttcttgaat gactgactgc caatccttgg aaatactcgc
     3421 cgatttgata aaatagcatc actgctacgg cttctggata ttcaccgatc aacatagcgc
     3481 caatcgtagc aacagacata agaaaattct catcaaagat ttttcctttg acgatattca
     3541 tcactgcctt cttcactaca tcatatccta tcagcagata aatcaataaa taccctatca
     3601 agcgaattgg ttcattaaaa tcaaataata gcaataaagc gatacctgca agggaaaata
     3661 aaatccggaa cagcgtattt ctctcttttt catttccatg ttcagatact ttttccttat
     3721 ctatttctgt tactttgaca tctggttcta attccttcac tacttttttt gtttcttgtt
     3781 caatcgtagc caaatgatct tcctctgcgt caatcgttag ttttccagaa gtgaagttta
     3841 ccgttgcttc cttgactcca ttgatcttct gaactccttt ttcgattttc attgcacagt
     3901 tcgcacaatc taaaccttct aatctgtaac ttttcatttt aatgacctcc tcgcctcaac
     3961 atatgaacat ctcttcataa ctaattataa atgaatggtt attcatatgt caaggtatcc
     4021 gaaagaaaaa atgaaaaaca tctgcatcgg aa
//
LOCUS       DACCQP010000137         4025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCQP010000137 DACCQP010000000
VERSION     DACCQP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.143"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(32..2503)
                     /locus_tag="IVO87_002778"
     CDS             complement(32..2503)
                     /locus_tag="IVO87_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7450902.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2776..>4025
                     /locus_tag="IVO87_002779"
     CDS             2776..>4025
                     /locus_tag="IVO87_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7450903.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYL"
BASE COUNT         1192 a          827 c          726 g         1280 t
ORIGIN      
        1 tcttttttac tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa
       61 attttttagc tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat
      121 attctaccaa tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca
      181 acagcaaaag tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga
      241 ccgaaaaaaa gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg
      301 gtgaacagat tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg
      361 tagaaacatc tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta
      421 cgatcatatc tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc
      481 ctctgattcg ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa
      541 acaaactaat caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg
      601 aaaacagtga gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct
      661 caatattgtt cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag
      721 aatcaagtaa gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta
      781 tactgcagtc tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta
      841 ctgtattgtt acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc
      901 caaaaactgt atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat
      961 caacgtactg atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg
     1021 gattatctcc agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg
     1081 ctcttgcttc ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt
     1141 ctgtcaatac tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc
     1201 ctttttcagc gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca
     1261 tttcgggcgc tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga
     1321 cagaagaaaa aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt
     1381 taacagcttc cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg
     1441 cttccaattg atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa
     1501 cagacatttt attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg
     1561 ccaatgtctc aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg
     1621 cacttaatgc taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca
     1681 atgctgcttc cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt
     1741 gagaaaaaag caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat
     1801 taagagaagc aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca
     1861 gctgggaaat atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa
     1921 caatgaaact gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc
     1981 ctgtcaataa agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa
     2041 tttgatatcc agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg
     2101 tttgttctat cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt
     2161 ccaagatctt tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa
     2221 ctggtaaaaa agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa
     2281 gaaagatcaa attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga
     2341 atgtgggatc gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg
     2401 ccgttaatcc ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg
     2461 ttactttctt ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga
     2521 tttcgttttc tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg
     2581 ttcctcacat taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt
     2641 ctataatatt ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg
     2701 tttcaactca aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac
     2761 caaaggagtc tctcaatgga taatcatact agaaaattac tcggattaac agataaacat
     2821 ttattttttg atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt
     2881 attaaaggga gattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt
     2941 atccgcaacg gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact
     3001 attttagaat taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg
     3061 aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc
     3121 atggaactgt acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat
     3181 gggacggtga cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc
     3241 cttccgacgg tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg
     3301 agttttattt gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga
     3361 ctttacaaac taactcaata cttcctgcgc tttcctagaa aggcccgttt aaaggtcaag
     3421 tacctagtga tggacatgaa tgccagctat tgtcagttgc ttaaaacagt ctttccaaat
     3481 gcggagatcg taactgaccg ctttcatatt gtccaacaca tcaatcgcag cttcaatcaa
     3541 ttacgtgtcc agatcatgaa tcgttttcga acctcacact ctgaaaatca gaagaagtac
     3601 cgtcggctca agcgatattg gaaactctta ctcaaagatt ccacgacatt ggagccactc
     3661 aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat cgttgatgag
     3721 ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt attacgctat
     3781 tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat ttcatctacg
     3841 ttacctcagt cgtttaaaaa gaaattcacg atcttccatc gctaccaatc gggtatttca
     3901 aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa caagatcaag
     3961 ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc acggatctat
     4021 ttaca
//
LOCUS       DACCQP010000138         4016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCQP010000138 DACCQP010000000
VERSION     DACCQP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.144"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IVO87_002780"
     CDS             <1..156
                     /locus_tag="IVO87_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP7450904.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVO87_002781"
     CDS             175..1452
                     /locus_tag="IVO87_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450905.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVO87_002782"
     CDS             1462..2118
                     /locus_tag="IVO87_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7450906.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVO87_002783"
     CDS             2118..3494
                     /locus_tag="IVO87_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7450907.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..3956
                     /locus_tag="IVO87_002784"
                     /pseudo
     CDS             3624..3956
                     /locus_tag="IVO87_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1612 a          437 c          705 g         1262 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatc
//
LOCUS       DACCQP010000139         3962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCQP010000139 DACCQP010000000
VERSION     DACCQP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.146"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..549
                     /locus_tag="IVO87_002785"
     CDS             <1..549
                     /locus_tag="IVO87_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450908.1"
                     /translation="IEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR
                     FEGVTGREISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKC
                     ITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFK
                     NKYQIKGIKLNQETIKKLRIGG"
     gene            552..779
                     /locus_tag="IVO87_002786"
     CDS             552..779
                     /locus_tag="IVO87_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450909.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            782..1609
                     /locus_tag="IVO87_002787"
     CDS             782..1609
                     /locus_tag="IVO87_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HAP7450910.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1621..1890
                     /locus_tag="IVO87_002788"
     CDS             1621..1890
                     /locus_tag="IVO87_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450911.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            2052..2354
                     /locus_tag="IVO87_002789"
     CDS             2052..2354
                     /locus_tag="IVO87_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450912.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2351..2662
                     /locus_tag="IVO87_002790"
     CDS             2351..2662
                     /locus_tag="IVO87_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450913.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2659..2877
                     /locus_tag="IVO87_002791"
     CDS             2659..2877
                     /locus_tag="IVO87_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP7450914.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2888..3703
                     /locus_tag="IVO87_002792"
     CDS             2888..3703
                     /locus_tag="IVO87_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP7450915.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3842..>3962
                     /locus_tag="IVO87_002793"
     CDS             3842..>3962
                     /locus_tag="IVO87_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7450916.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETG"
BASE COUNT         1486 a          615 c          879 g          982 t
ORIGIN      
        1 attgaaattc gttttattga tcctagacgt ttcacagtac agcaacgaaa cttcatctat
       61 gcactcatag gcgatatttt catcgataca ggcatgccaa cggacttctg gaaggaattc
      121 ttttacttcc gtttcgaagg tgtcacaggg cgcgaaataa gcctcaaaga tgaatcgaat
      181 acgactgtga gtgatgccaa tatcttagca aatatcatct tagattttat ctttgaacat
      241 catattcctt tcaaagaagg ctatgagatt ttaccagcga atcaagaata ttacttctac
      301 aaatgcatca caaaaagagt ctgctgtatc tgtggcaaaa caggagctga tatcgatcac
      361 tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcattcaga gtacacattt
      421 gcagcactct gtagaattca tcacacggag aaacacaaaa taggtgtgac caatttcaaa
      481 aataagtatc aaatcaaagg gattaagtta aaccaggaga caatcaagaa attaagaatc
      541 gggggctaat gatgattaat ttaaacgcag tagcgtttga acaaacatgg cgcactaagt
      601 ataaaaaaat gagtccaaga gacaaattgt tcttagaaat catgacattt gctttcattg
      661 gtacacaagc tgaacaaagc gatattagtg ttgaaaaaat taagacgaat agattagtta
      721 atggaattac agagacttgt taccagtaca cgattatcgt cgtggatgag gaggaataat
      781 tttggcagaa agaagaatgt tcgcaaagac aattattgat tctgatgcat tcctagatat
      841 gcctttatcg acacaggcat tatatttcca tctatcaatg cgcgcagatg atgatggatt
      901 tattaataat cccaaaaaaa tacaacgtat ggttggttgt ggtgatgatg atttaaaact
      961 gcttatggca aaaagattca tcttagtttt cgagagcggt gtgatcgtca ttaagcattg
     1021 gaaaatacac aactatattc gtaatgatcg ttacaaaccc acgttatatc aggatgaaaa
     1081 agctttacta gcagacaaag ataacaaagc ttatacattt gcagaagagc tttctaaaca
     1141 tgatgaaaaa cttggtatac cagatgacaa ccaagcggta taccaaatgg atacacaggt
     1201 taggttaggt aaggataggt taggtaagga tagtaaagag ataaaagatt taacgccttc
     1261 gaaaaaatcg aaggctaagc ccatccgtca taaatacgga gagtataaaa atgttctttt
     1321 gtcagatgag cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat
     1381 cgaacgactg tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac
     1441 tattcgaagt tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa
     1501 gcgcacagga agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa
     1561 gaaaaaagcg ctagaacgag ctaatagaca atcaaaagca ccattttaag aggtggaaaa
     1621 ttgaaaatcg attatctaga actaattaat gaaatagcaa agtataaaac tggtgaggaa
     1681 atagaaatcc tgagagacgt atatgatcaa ctcgaagaag ctggaatcga aggaattaag
     1741 aatgatcgtt cgagttggag taaactcaga tactatttcg cactttatat cgatacaaca
     1801 caattaagaa atttagcata taccaaatta ctatttgttg attgcgttaa aggattgcaa
     1861 aaacatctta gtgaacttga gcaggtgtaa tcagatggat ctaaaaacat ttacagcaca
     1921 gatcgaacta atgcatcaag aagctttaag acaaagcgcc tcgtacgaag acaagtggct
     1981 caacacgttc catggtggac gtgagagcgc acttgatcaa gtactcaaat tattgaaagg
     2041 ggaacgtcgg gatggataag aaagcggcaa tgaaacgaat catcgaactg acacattctg
     2101 agaattggca agaagacaaa gaaatagtta cagaagtcca aaagctcggt aaatcaatgt
     2161 ggactgaaaa gcccaaacgg agaacgccga gaaagattgc aatttggcat ggtgatcgaa
     2221 ttctagtaac aggtactgct gaacagttat ctgaaattac tggtctgagc aaaaacatca
     2281 tctgggatag agcaaggagc ttatggattg attcaaaagg acgacaattt aggtatgtgg
     2341 aggagaaaaa atgaacgaac taatcacaaa agtagagcag tgggctaaag acaagggact
     2401 ggatcaagca gatccaaaag cacagttttt gaaagtagct gaggaattcg gggaaatcgc
     2461 ttcagcaatg gcaagaagta atgatgagct atttaaagat agcgtaggag acgtaatcgt
     2521 cacgttgatt atcctttcca tgcaaaaagg gacaaacgta caagagtgtt tagaaatggc
     2581 atacaacgaa atcaaagggc gcacaggaaa aatggtagat ggtgtattcg tgaagtcgag
     2641 tgatttggag gacagcaaat gataggttta acaatatttt ggtgtgcgat tggtggttta
     2701 ctagcatttc taggatacta cctcatctgt gaaagtagaa aaggaagaaa atatacagtg
     2761 acaggtattg tcctgttaat tatcggaatt ctagttgcag ttggagcaga actgaatctt
     2821 tatgtaaatg gtacacaaga agatttggtg cgatttatgt tttggatgaa gaattaggag
     2881 gaaaaaaatg aacgaaaaca aattaatcaa attaggtgta gcaggagcag taatcgtagg
     2941 tattggagtt atcggaggat ttaagttctt cgaaaaaatc gataatggat atgtgggtgt
     3001 gcgctattca atgaacggcg gtatcaaaga tgaagcactg acgcaaggtg tgaaatttgt
     3061 agggattgac aaagtgatcc aatatccaat tcgcttgcaa actatccaat caaaaaatat
     3121 ttcagtatct acaagcgacg gcaaaaagac aacgattgat atcaaatatg actacaaagt
     3181 tgattcaact aaagcagcaa aaatgtacaa ggaatttggg aatatcactt cggaagatat
     3241 cgaaagtgga tggttaaaat ctaagcttca aaaggtcgct cgtgaagttt atgcgaaata
     3301 tagtctgctt gatgtccttt caggagattc ctctaaagtt gaagctgagg tattaacgaa
     3361 ctttgctaaa tcagttgaat ctaaagggtt tgaagtcgaa gacgtaacac ttggtgttcc
     3421 agatgtcgat aaagaaacac aaaaatcaat cgatgcgatc attcgagctg gtcaagaaaa
     3481 tgaaaaagcg aagctagatg cagaaactgc aaaaactcaa gctgatagtg aagcttacaa
     3541 gaaaacaaaa gctgcagaag cagaggcaga atctaatcgc aaagtcgccg aatcagtaac
     3601 agacaatttg attcgttatg aagaagctca agctcgcaaa aagcatggat gggtaacagt
     3661 aaatggagca gatactgtag ttacggatga agcaggcaaa taatatggga ttctttatgg
     3721 ctaaaattct cttgttctta ggtttagtag gagcagcata tctcgtgtat gcgctccttt
     3781 cccaaactga tgacaaagaa gatgacaaca acgatgaaat gaaattttag gaggagaaat
     3841 aatggatctc attacacaat acagtgatat catcctcaag aaaatcatgg caaagattca
     3901 gaaagataaa aaagcaaaag aacgagcgga attagttaag ttggaaatgg ctgaaacagg
     3961 ag
//
LOCUS       DACCQP010000140         3898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCQP010000140 DACCQP010000000
VERSION     DACCQP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.147"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IVO87_002794"
     CDS             <1..182
                     /locus_tag="IVO87_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450917.1"
                     /translation="ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRN
                     SSYGSDYTFAITYTI"
     gene            345..791
                     /locus_tag="IVO87_002795"
     CDS             345..791
                     /locus_tag="IVO87_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450918.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            793..930
                     /locus_tag="IVO87_002796"
     CDS             793..930
                     /locus_tag="IVO87_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7450919.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            968..1261
                     /locus_tag="IVO87_002797"
     CDS             968..1261
                     /locus_tag="IVO87_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450920.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1258..1482
                     /locus_tag="IVO87_002798"
     CDS             1258..1482
                     /locus_tag="IVO87_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7450921.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1479..2504
                     /locus_tag="IVO87_002799"
     CDS             1479..2504
                     /locus_tag="IVO87_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7450922.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            2694..2767
                     /locus_tag="IVO87_002800"
     tRNA            2694..2767
                     /locus_tag="IVO87_002800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2728..2730,aa:Ile,seq:cat)
     gene            3444..3898
                     /locus_tag="IVO87_002801"
                     /pseudo
     CDS             3444..3898
                     /locus_tag="IVO87_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1353 a          665 c          776 g         1104 t
ORIGIN      
        1 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
       61 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
      121 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
      181 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
      241 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
      301 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
      361 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
      421 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
      481 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
      541 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
      601 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
      661 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
      721 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
      781 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
      841 actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa
      901 atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag
      961 ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt
     1021 ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac
     1081 tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc
     1141 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc
     1201 agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg
     1261 aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct
     1321 actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc
     1381 atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac
     1441 aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta
     1501 ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta
     1561 ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca
     1621 aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga
     1681 aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg
     1741 tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg
     1801 cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc
     1861 gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg
     1921 atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg
     1981 tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa
     2041 agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca
     2101 agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa
     2161 atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat
     2221 ggcacattgc taactataaa tacgagtata ttttcattat ggactacaat actgggaaag
     2281 aattagctcg agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata
     2341 cttctggaaa tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag
     2401 tctatgtaat gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag
     2461 atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg
     2521 aggagcagta cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta
     2581 tcgcatatct tcactatcac ccataaatag tcacactcca agctatgcga taacaggttt
     2641 gttgccacac attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct
     2701 tagctcagtt ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     2761 gggtacatta acgtagccat ttgaatcgtt ctgtgttaga atttttttga agagtattat
     2821 acaagctaaa gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta
     2881 tggattaatg aaaggatgtt tcacatagtt atacttctgt atatttgaaa agttttactt
     2941 tgatttttaa atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta
     3001 aagcgcaccc caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga
     3061 agccattaaa aagcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga
     3121 gtggtagtta attagtgact tatttttgat tttatagcac tgatactata aaatatagat
     3181 atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt
     3241 gataaaattc gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg
     3301 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca
     3361 cgtcagtcat gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata
     3421 aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg aagaacctat tcaaaagaat
     3481 tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa attattcgag
     3541 agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct
     3601 cattcaaaga aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga
     3661 aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac
     3721 gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat
     3781 tctaaatatt tctcgtcaaa cctactatta tcaagcgaaa ccgatcgaaa atgagtccga
     3841 cttagaagag atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccga
//
LOCUS       DACCQP010000141         3862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCQP010000141 DACCQP010000000
VERSION     DACCQP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.149"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IVO87_002802"
     CDS             <1..391
                     /locus_tag="IVO87_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP7450923.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="IVO87_002803"
     CDS             388..945
                     /locus_tag="IVO87_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7450924.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            942..1238
                     /locus_tag="IVO87_002804"
     CDS             942..1238
                     /locus_tag="IVO87_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450925.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="IVO87_002805"
     CDS             1315..1728
                     /locus_tag="IVO87_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7450926.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(2186..2461)
                     /locus_tag="IVO87_002806"
     CDS             complement(2186..2461)
                     /locus_tag="IVO87_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450927.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="IVO87_002807"
     CDS             2915..3121
                     /locus_tag="IVO87_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450928.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="IVO87_002808"
     CDS             3317..3484
                     /locus_tag="IVO87_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7450929.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..3854
                     /locus_tag="IVO87_002809"
     CDS             3510..3854
                     /locus_tag="IVO87_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7450930.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1336 a          599 c          828 g         1099 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     3841 gtgaaaggtg gtaagtgcat ga
//
LOCUS       DACCQP010000142         3779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCQP010000142 DACCQP010000000
VERSION     DACCQP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.150"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..236
                     /locus_tag="IVO87_002810"
     CDS             <1..236
                     /locus_tag="IVO87_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450931.1"
                     /translation="MGKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDME
                     VAVAVYSTMMFKQARDAYQKEFEKANKEKKLQK"
     gene            239..421
                     /locus_tag="IVO87_002811"
     CDS             239..421
                     /locus_tag="IVO87_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450932.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            439..3771
                     /locus_tag="IVO87_002812"
     CDS             439..3771
                     /locus_tag="IVO87_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7450933.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
BASE COUNT         1334 a          574 c          836 g         1035 t
ORIGIN      
        1 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
       61 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
      121 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
      181 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
      421 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
      481 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      541 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      601 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      661 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
      721 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
      781 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
      841 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
      901 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
      961 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
     1021 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
     1081 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
     1141 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
     1201 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
     1261 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
     1321 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
     1381 aatacgatca agcctgtttt tacacaatat acgtcaatgt ttaaaggact tggatatgat
     1441 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
     1501 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
     1561 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
     1621 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
     1681 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
     1741 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
     1801 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
     1861 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
     1921 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
     1981 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
     2041 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
     2101 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
     2161 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
     2221 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
     2281 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
     2341 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
     2401 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
     2461 ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt
     2521 gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac
     2581 attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt
     2641 aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct
     2701 gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac acaatggata
     2761 agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca
     2821 tggtggaatg gtatatggac atctattagt actacggctt caaatatttg gaattcaatt
     2881 agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa
     2941 gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt
     3001 tggaattcaa tcagctctac agcatcaaat atctggaata gtgtgactag tagtataagc
     3061 aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg
     3121 atcagcggaa tttggaacgg tatcaaaaat actgctttga atctttggaa tggaattaca
     3181 agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat
     3241 ctaacaggta ttgtttccga catattcaat aatgttaaaa gtacaattgc taacatatgg
     3301 gaaggcatca aaaagactgt tagcgctcca attgattgga ttagagacaa aatcagtggc
     3361 atttttgata atttgaatat ttctatacca catattccgt taccggaatt tatcatggaa
     3421 ggcagcttta acccgctaaa aggtcaaatc ccccatttgc gtgtaaaatg gcatgctaaa
     3481 ggaggtatct ttactaaacc aactttacta ggtggaatga acggtgtcgg tgaagcagga
     3541 cctgaagcag ttttaccttt gaaaagatct gttttgcaag aaattggtga tcgtatcttg
     3601 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
     3661 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
     3721 attgatagca ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta aacaggaga
//
LOCUS       DACCQP010000143         3679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCQP010000143 DACCQP010000000
VERSION     DACCQP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.151"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(183..1502)
                     /locus_tag="IVO87_002813"
     CDS             complement(183..1502)
                     /locus_tag="IVO87_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7450934.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1759..2229
                     /locus_tag="IVO87_002814"
     CDS             1759..2229
                     /locus_tag="IVO87_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7450935.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2451..3554
                     /locus_tag="IVO87_002815"
     CDS             2451..3554
                     /locus_tag="IVO87_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7450936.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1181 a          630 c          789 g         1079 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaattaaa agtatgaatt attcaggtag aatctctgaa gaataaatca
     1681 cgtgatcgtg acgataaaat acacttacgt caggtgataa aaaatacata agtagaaaac
     1741 taagaggagg aacagaaaat gaaagaagtt caattgatca gaaaaagcga actatcagaa
     1801 ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct atacattaaa gcttggtgcc
     1861 aacaaagcga taatctctga gttgacagta ggcggtcttg tggacagtct ggcgcttgct
     1921 gaaggtttta ttggagaaga tatttatgag atgttttcag aaatccgtca gttaaaaaaa
     1981 ggggaaaact gcatagaggt tcatcatgag agcccgaatg tgagattcaa acgtggtgat
     2041 aatgagatga tcttcaataa tcatgtgtcc gatcacaccg agctatatgg aatcgtgaat
     2101 caaatactaa ctgagctttt tggattggga ccttatgcgt tcaaggaaga aaacggtaat
     2161 ccaaaattaa acgaagaatg gcaagaaacg attgaaatac aaagaaacaa cccgcactta
     2221 tttcaatagg catccacagt atttcgtaaa agggactttg acacgacttt tgtcaaaatc
     2281 ccatcgtttt ttatctattt gtgttctaaa catttctttc agagtagtaa caactggaat
     2341 gactgaaatg taaacagatt atttgcctgt atcaatacgg aagagtaata taaagtgtgt
     2401 aaacgctatc atattattca ttcaattttt tatagaggga aggcattgaa atggaaaaaa
     2461 cgtatcaagc aggtacaaat gaaggaatag tggattttat caatatggaa gatttagaaa
     2521 tagccgcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
     2581 acttatttac ttatcaagaa aatgaacggg cattcaatca tcagctgatt attcctcatg
     2641 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacaaagaa acgctgactg
     2701 caccaatcat catggcacca gttgctgctc atggcttagc tcatgtgaag gcggagaaag
     2761 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
     2821 gtacattaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
     2881 atatgagcaa agatgatggg atcaatttgg atattcttga agtggccaag cgtaatggag
     2941 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
     3001 gaaatgggtt taccttccca ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
     3061 gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa gcttagtcca aaagatgtag
     3121 aatttattgc tgctcattct gatttgccag tctatgtgaa aggtgtccag tcagaagaag
     3181 atgtctatcg ttcattagaa tctggtgctg gcggaatctg ggtatctaat catggtggcc
     3241 gtcaactaga tggtggtcca gcagcgtttg attcgttgca atatgtggca gaagcagtag
     3301 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg cggacagcat gttttcaaag
     3361 caatcgcatc aggtgcagat cttgttgcta ttggcagacc cgttatttat ggattgtctt
     3421 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
     3481 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
     3541 atcgttttat ttaagaaaca aatgacgaaa aaaataggag tgcggcagat gatgccgtac
     3601 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
     3661 tgaaatattt tttcatact
//
LOCUS       DACCQP010000144         3610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCQP010000144 DACCQP010000000
VERSION     DACCQP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.152"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            488..814
                     /locus_tag="IVO87_002816"
     CDS             488..814
                     /locus_tag="IVO87_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450937.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(931..1275)
                     /locus_tag="IVO87_002817"
     CDS             complement(931..1275)
                     /locus_tag="IVO87_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450938.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1351..2598)
                     /locus_tag="IVO87_002818"
     CDS             complement(1351..2598)
                     /locus_tag="IVO87_002818"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7450939.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2595..3401)
                     /gene="proB"
                     /locus_tag="IVO87_002819"
     CDS             complement(2595..3401)
                     /gene="proB"
                     /locus_tag="IVO87_002819"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7450940.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT         1065 a          704 c          719 g         1122 t
ORIGIN      
        1 caggaggttg taaaatcagc gttttgtcct gagtattaga gaaacaaacg gaaaaatagt
       61 tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acaccgttta
      121 acagaggttg taaaatcagc gttttatcct aagtattaga gaaacaaaac ggaaaaatag
      181 cttctcatat tttaccgttt tttgagctaa taccgagggt cagctgattg aacgccgttt
      241 accaagaggt tgtgagactg tcgtttaact ctgaatcaca aggaacaatt tcaaaaaaca
      301 gtttctcatg tttgttgaat attgtgactt atgattgagg agttggcaga agaacaccat
      361 ttattcggaa aaaggggatg tgacacgatt tttgtcacat tccctttttt tctcaaaata
      421 atgaaactgg aaaattttca gtaggtaagc taaaattcat ttaagaaaaa gaatgggggg
      481 ataatagatg ctggaagaat attttcagga agtcgggttg acactcgttg aattacccat
      541 tgaattttca gatgatcggt tgatttatcg tggctatatc aaagaattgc caataatcaa
      601 tgcggaaggt gaaactagaa aagcgatgca gcaaaaaata gctggtatgt atcaggtgta
      661 tcgggaacag ctactggctg aatcagcgga agaagaaaaa gaaaaaacga tgaacttgtc
      721 catggatgag ctgcttcgtt attatgatgg cgaaacattt gacggatttt cgcttcttaa
      781 cgaagaggaa acagatttac caggcagctg gtaggcgaac gccgtttata tggaaaaaga
      841 ggctgtgaca aaatctttgt cacagcctct aaaaagtccg gcagtttttt gccgatcttc
      901 aaggcactct acttatcgta cggatatttt ttagcggaaa gtccgattca agtggttttt
      961 attcagctga tatttataag ataagtgaaa aatcccagca atcagtagcg taatcgtata
     1021 agcgattgaa tgatcgaaaa aagaatagta attgattgtc gctaataagc ataatgcaac
     1081 catgtaacta atgtgcaagc gtgtaaccag ccctttttgc ccgattttgt caaaatactg
     1141 cgaccatttt tcccgttgac tccctatgct tcgttttttg aattgaaaga aaagggataa
     1201 aataccgact cccagaaaag cagcgatatt cagagaaata aggtaaataa aaaaaatcat
     1261 taaaatttca aacatattga cacctttcta gttggataga acctttacag aaaaaaggca
     1321 aaaggcggga ataatgtttc acacgaaaca ttattcgcgg atttgtccat cgccataaat
     1381 gatatatttt gtggaagtta attcatttaa ccccattgga ccacgggcat gcaacttttg
     1441 agtagagata ccaatctctg caccaaaacc aaataccgat ccgtctgtaa aacgagtgga
     1501 ggcattgaca tagacagccg ccgcgtccac ttcatttaag aaacgttgac tagccgcata
     1561 actatctgtc acgatcgttt ctgaatgttt agtattatgc cgattgatat gtgcaattgc
     1621 ttcatccaaa gaatcgacca ctttgacagc aaggatataa tctagatatt cagtatccca
     1681 atctgtgtct tctgctaaag cagccgtctc tagatattct aacgcacgag catccgcacg
     1741 taaagagacc ccgtgttcta ccagttcttt ttcaatagct ggcaagaaaa aaggtgcgat
     1801 ctcagaatgg atcagcaatg tttctgccgc gttacagaca gatggacggg aagcttttgc
     1861 attgacgatg atgttcactg ccatagataa ctgtgcttct ttatcgatat atacgtgatt
     1921 atttcctgta cctgtttcaa tgataggtac cgttgcctgt tctttgactc gttggattaa
     1981 ttttgctccg cctcgaggaa tcagcacatc taagtattct gtcaatcgca tcaactgtcc
     2041 tgcagtttca tgagaagtat catcgacaaa ctggatggca taaggagatt cgccagcact
     2101 gaccagtact tgctgcaata attggactaa taattgattc gagaagaatg cttctttgcc
     2161 accgcgaagg ataacggcat tccctgattt gaatgctaga ctagctgcat ctgtcgtgac
     2221 atttggacga gattcgtaaa tgatgccgat cacgcctagt ggcacgcgct ttttgccgat
     2281 tctcaatccg tcggtgttcg tccacatctc atctactttt ccaataggat cgtctaacga
     2341 agcaattttt tcgatctctg tagccatggc attgattcgc tcaggtgtca gctgcaagcg
     2401 gtcttgcatc gtttcactga tattgttttc gactgctttt tctaagtctt tatgatttgc
     2461 ttcaatgatc gcttgttgat tctttttgat ggtttgtgcc atttgaacaa gtagctcatt
     2521 tttgcggttt gtaggcataa gtgctagttg ttgcgcgcta tcttttgcct gttgtcctaa
     2581 ttgcatcaag tcagtcattt caagtcctcc ttaaataaag tacctacttc ttttcctgca
     2641 agaatatcaa aaataattgc cggatttttt ccatttgcta aaatcattgc gctgtttgct
     2701 tcaaatatcc tagaagcggc ttttaattta ctctgcatac cgcctgttcc aaatgtgcta
     2761 ccagcaccac ctgcttgtgc cagaatcttt tcatcgattt tatcgatata gcgatacatc
     2821 tgagcctcgg gatttgtagt cggattatct gaataaaacc cgtcgatatc agaaaggacg
     2881 atcaaaaggt ctgccgcgac aagttttgcc atgatggctg acagctgatc attatcgcca
     2941 aattttgtca gatgatctaa ttcatccact gctacagtat cgttttcgtt cacgataggg
     3001 atcgtgtcca taatcaaaag ctgttccaat gtattcctca catttttacg actttcagga
     3061 taatctacca cgtctcttgt cacgaggatc tgtgccgtct gttgattata tacactgaat
     3121 ctttgattat agatattcat cagttcggct tgaccaacag ccgccgcagc ttgctgttca
     3181 ggaatcaaag tcggacgttc tgacaaatgt aatttatgca taccaacgcc tacagctcct
     3241 gatgagacta agatcacttc ctttccccga tttctaagat cagaaaggac aaaagctaac
     3301 tgatcgattg ccgcaagatt gatatttcca ttgggataaa tcaatgtact tgttcctact
     3361 ttgacaataa ttcgttttgc atttttaatt tctttacgca tgtgacacct caatcaaata
     3421 tccactcatt aatttcgttc tattctactt gttttgacga gtaaagtcat gtaaaaaata
     3481 gtttttttgt acaacttgca gtcgtttgga aaaagaacga attaatggtt tactttaaga
     3541 tagcagaatt tcttgaagaa tttgtaaaga aagtctttta cagcctcgga tatgttagaa
     3601 taggagtagt
//
LOCUS       DACCQP010000145         3610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCQP010000145 DACCQP010000000
VERSION     DACCQP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.153"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..214
                     /locus_tag="IVO87_002820"
     CDS             <1..214
                     /locus_tag="IVO87_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7450941.1"
                     /translation="WKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEK
                     YKEILEEYLRRTANDKKTRSIQHGFI"
     gene            174..419
                     /locus_tag="IVO87_002821"
     CDS             174..419
                     /locus_tag="IVO87_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450942.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            440..529
                     /locus_tag="IVO87_002822"
                     /pseudo
     CDS             440..529
                     /locus_tag="IVO87_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            604..1071
                     /locus_tag="IVO87_002823"
     CDS             604..1071
                     /locus_tag="IVO87_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7450943.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            1346..1582
                     /locus_tag="IVO87_002824"
     CDS             1346..1582
                     /locus_tag="IVO87_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450944.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1606..1947
                     /locus_tag="IVO87_002825"
     CDS             1606..1947
                     /locus_tag="IVO87_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450945.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1940..2119)
                     /locus_tag="IVO87_002826"
     CDS             complement(1940..2119)
                     /locus_tag="IVO87_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP7450946.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            2341..2988
                     /locus_tag="IVO87_002827"
     CDS             2341..2988
                     /locus_tag="IVO87_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450947.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            3248..3592
                     /locus_tag="IVO87_002828"
     CDS             3248..3592
                     /locus_tag="IVO87_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7450948.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1329 a          492 c          725 g         1064 t
ORIGIN      
        1 ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag ggttcgatca
       61 gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa
      121 atttgtagag aagtataaag aaatattaga agagtatttg aggaggacag caaatgataa
      181 aaaaactcgt tcaattcagc atggatttat atgatatcga atcaggagca acactatctg
      241 tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt ttgtgggtag
      301 ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa gcggttgagt
      361 ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac agcgaatgat
      421 accgaagttt agagcgtggg atagagaaaa agacagaatg aaggcagtag ctttggttga
      481 atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga
      541 ttcctccgac tatgaataat atttccgaca taagtattat atcataattg ggggaatcag
      601 aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg
      661 gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga
      721 gtagggcaac ctcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca
      781 gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa
      841 tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag
      901 cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc
      961 atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac
     1021 agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt
     1081 acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa
     1141 tcacttacct attcaacgtt gtattatgat agtgtcgaaa gattaggaaa caggatcgac
     1201 aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca
     1261 gcggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct
     1321 atcatccgtt gctgtctatt aatttatgta ttggagggta tataaaatga agatcgcttc
     1381 cgaaaaaatt gatcaactaa caattttagt aaaccaaaga atcgaagata ttatcaaaga
     1441 attcgaaggt gaaaagatta agaacataaa gcttacttta atggatcata agttcgaatt
     1501 aaattcgaca aaaaggaaga tcgttgagct tatagaagaa gaaatagctc tccagatcaa
     1561 tgagcaacaa ttagctaatt aatttatgta ttggagggga aatgaatgga agtcgaacaa
     1621 ataaagtatc aaggtatgga tcgggaacgg ttgattgata ttattgagca acaacgttta
     1681 gaattgatcc aaaaagaagc tgtatgtaaa gagtacaaaa aacatttgga acaagtaatt
     1741 gagtatcatt cagtagaaaa gtacagatca gtggtgcaaa aaaatagagc aacgagtcct
     1801 gattctcgtc aagatgtatc gattagtatc aagacgccag tatttaattc tgttaaatca
     1861 gaaaagctag aaagtatctc aactgctttg gtagaaaaag cagctgaaat gaactcccta
     1921 gcaaatgaag ttagtcgtct tatttagtca tatcgataac ttgagagtct ttggtgattc
     1981 cgttttttat agaattaatg gtttgttcag cggaatattt agtcaagtat gtttcgctag
     2041 ttgctactac ttcattgtta ttggacttaa taacgaagta atattggcca ttagttgctt
     2101 ctcttataac aaagtacaaa tttttcacca cctataattt atttcagcgg accactcgct
     2161 gataattaaa attataagct atgtataaaa tttcacaata tcaatttgtc gctgtggcgg
     2221 aaagggtaga cgcaataaag gatgaatggg aaaagtcaat gagtaattat ctgaaattta
     2281 caagtcccct gtttagttat ctaagattat ttgataatat tatttctgag gtgatagaaa
     2341 atggtgggcg tagttaaatt tcttgggttt acatttgatg gatatggtac gttaggcgaa
     2401 tgggtaggta ttattttgat agtgattaca attcaacaaa catcaaagta ctactggtat
     2461 tctaatagtt ctaaaattgt atcaagaata actgaaggaa taaccaaaaa agatattact
     2521 gtcgtcggta aatatgggtt tactataact ttattaaatg aagggaaatc tgaaaccgca
     2581 ataaagtttt gcgggattat aggtaaatat aacattttcc aacgttttat cagaaagcat
     2641 tggtacaaat atatttacaa ataccctgta gttaaaaagc ttattgaaaa atatgtggac
     2701 cacgaaatat cttatctcaa tccaacagac cttattaatc aagaattcca gaatattgga
     2761 tacttagaaa aaggtctaaa aataccaatt gtaaatgaaa gtttagttac tgcaatggta
     2821 gctaacataa agaacagcaa gaaattagaa aagagatata aaaaatgtaa aaaattgaaa
     2881 ttttcctttt tattcttaga atataatggc aaagaatatt atcaagatat ttatgttttg
     2941 cgaggctcga acttatataa agacattaaa aataagattt cagtatgaga ttttttaaaa
     3001 ttcgtagatc actctttgag tggtcttttt attttgcgta aaggaggcac gacgatgtat
     3061 agaccacaat acttagaaca gaagcatgaa gtaatcactg tgcaaaacgg taacggtgag
     3121 atagtacgaa agtatagaag accaataaag agcgatacat ataaacggaa ggaaagcaat
     3181 gaagttattc cattgtatgg caaaagaata gctaagtatt aaataagatt gcgaaaggag
     3241 atgggacatg accgaggaat tctatagatg gttattacag ttgataagag aagatcgttt
     3301 ggttaagttc tatcagtctc ctaaatggcg caggcttaga gagaaagcga tgaaacgaga
     3361 tcactatgaa tgccaagagt gtagaagact aggtaagtat catagagtag agaacgttca
     3421 tcatataaag gaagtcaagg atagacctga cttagcttta gatttagata atcttatttg
     3481 tttatgtgtt gaacatcata atgaagttca tggcagatat cttacagcgt tagataaaca
     3541 agagaagaag atagaaagct tcgctaactt cgatgcaagt gaaaggtggt aagtgcatga
     3601 tcatcaatga
//
LOCUS       DACCQP010000146         3540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCQP010000146 DACCQP010000000
VERSION     DACCQP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.154"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..226
                     /locus_tag="IVO87_002829"
     CDS             <1..226
                     /locus_tag="IVO87_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450949.1"
                     /translation="TGNFLRWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYF
                     APNGPGWTMNIGIYYTYNPVAGTYYVVQNI"
     gene            351..2702
                     /locus_tag="IVO87_002830"
     CDS             351..2702
                     /locus_tag="IVO87_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7450950.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2782..3177
                     /locus_tag="IVO87_002831"
     CDS             2782..3177
                     /locus_tag="IVO87_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450951.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1237 a          442 c          703 g         1158 t
ORIGIN      
        1 tacaggcaac tttttacgtt ggaaatccta cattgacgat ggaagagtag gaaatgtaaa
       61 tcaagccaac gcaactaaat ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg
      121 attcaaatac tttgcaccaa acgggcctgg ttggacaatg aatataggta tttattacac
      181 ttataatcct gttgcaggta cgtattatgt tgttcaaaat atttaataaa acagcacaaa
      241 gagatttctt tgtgctgttt tattattttt agttgttgac aaacactaaa atataaaata
      301 taatcaacac ataaacgaac gtatgttcgg caagaaagca ggggattttt atggatgaga
      361 tggttttaag tactcagaaa tggctaaaca agaaatacag taatgttaca gggtttgata
      421 aggttccaga aaatgggcga acagggtggc ctacaatcta tggacttatt gaaggactac
      481 aagtagaact tggtattact aatctagtag ctaattttgg acctaccact gaaaaaatgt
      541 acgataatca ggttactcct aaatggggca aaaatctacc taaaaatatt gtatttctta
      601 ttcaaggtgc cttttggtgt aaggggatta atccaggtgg cttcgatggg gtatatactc
      661 catatttaga tactgctgta aaagagttgc agactgatgc agggtttaat ggaagcgctg
      721 taactggagt tttggatgcg aaatgggcca aagcgttatt tgatatgagt gcttttgttc
      781 ttgttcccgg cggagatagt aaaattagaa aaatgcagca atggttaaat gtaaattatt
      841 tagaatacac gggaataatg ccttgtgatg gtatttatca aagaaattca aatcaagcat
      901 taatttttgc attacaagct gaactaggat attctcccag tgaagcaaca gggtcttatg
      961 gtaatgggac aactagtaaa acttatcctg tgtctgaagg taactcggga aactatgtaa
     1021 gaataattca atatggatta tatgttaatg gttattttga agacggtaca ttcgatggta
     1081 ttttcactaa atatatgggt ttagaagtac tggcattccg aaaatttatg gtattgcctg
     1141 aatatactga aatagcagat gttgttgtaa taaaaggact attgtctagc gctggagata
     1201 ttagaagatc tgcagatggc gcagatactt ctacgcaatt aactagatct caaatccaaa
     1261 cattggttga taatgatata aaaatagttg gtagatatct tactggtact gttggaattg
     1321 gaaaagatga acgaaataag tatctaacta cagaggaatt gaataatatt ttttctaaaa
     1381 atttatctgt gtttcctatc tatcaagatg gaggtgcagc attagcttat tttacttatg
     1441 atcgtggctt atcagatggt aagaaggcta tagatgcagc aaaaaatcta aatataccat
     1501 taacaacagt tatttatttt gctgttgatc tagacatgtt aggggaagaa attcttgcat
     1561 atgctggaga atattttaag ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg
     1621 gtgtttacgg aacaagaaat gtttgttctc aaataatcaa taacgggtat gcttcgtttg
     1681 catttgtttc agatatgtcg actggttata gtgggaattt aggttttcct atgcctagac
     1741 aatggtcttt tgatcaattt gttgaattta ccataggaag tggcaacgga gctgttggta
     1801 tcgatttaat cgctacatcg ggtagagata gcggatttaa tgagttatca aatgatacta
     1861 ataatgataa ttacattgcc aaatacaatc agaaggttat aaatcagatg gtacgtgcgt
     1921 atcaatatct atcacaagct ggtcttgata atccttggaa ccctcatttg acattttata
     1981 gatatgtaaa ttattctggt ttgtcatggg acatcatttc ttctcctgta acagagcatg
     2041 atagaaaaat ttatgatgaa tataggaata aattaactaa tagcgagggc ctgtataatt
     2101 attttataga tcctaatact ggttcaataa taggtttgcc tcacttaatt gtgacgttac
     2161 aatcacagat gtttctagcc gacacaatca atgatagtgt ttctgacttt gcaggttggt
     2221 taggagattt aatgacttgc tggggtgaag ttaagaaatt aggaatacaa atggagcaag
     2281 gtgtatttgc attagtaggt acggctgcag gtggaacatt tagtctagaa gatttatacc
     2341 aagatgttga cgcggtaaat gtttatacct tagcaaaaag aaattctgat tcaacagctt
     2401 tatcggcttt aaaaacgtac tattgtgata agggtgctat tgatagattt aatcaatttg
     2461 ttaagaacag atttggttct tcagataaaa tcgcttcagt aacaaatagt ttattagaaa
     2521 atgacgaaac agttgatatc ttaggtgtag ggataatttc atttttcatt gataaaatag
     2581 ttaataattc acttgggaca gattttactt atcttgattt tattggagat gaggggaaag
     2641 ttgcaaatgg attttctgaa aaaattattc aatttcaaga aaatagaagt aaacttaatt
     2701 aagaatcgtt tactatttaa acagtgtcct tttccttttt aaatatatac gataaaataa
     2761 gtatatgaat tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact
     2821 caaaataata aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga
     2881 atagtagtac ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa
     2941 caaatacaga gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat
     3001 tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt
     3061 gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta
     3121 tcaaaaagat ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag
     3181 tgttaaaaaa attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc
     3241 atggaagaca taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc
     3301 tgccattttt atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt
     3361 ttcaaaaagt atactttttg acatgccttg attttctttg tttatgtatg gcataataac
     3421 cacataatac aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg
     3481 aaaaaagggt agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat
//
LOCUS       DACCQP010000147         3531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCQP010000147 DACCQP010000000
VERSION     DACCQP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.155"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IVO87_002832"
     CDS             complement(25..144)
                     /locus_tag="IVO87_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7450952.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IVO87_002833"
     CDS             complement(159..1343)
                     /locus_tag="IVO87_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7450953.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IVO87_002834"
     CDS             complement(1599..1853)
                     /locus_tag="IVO87_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450954.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IVO87_002835"
     CDS             complement(1878..3020)
                     /locus_tag="IVO87_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP7450955.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..>3531)
                     /locus_tag="IVO87_002836"
     CDS             complement(3080..>3531)
                     /locus_tag="IVO87_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7450956.1"
                     /translation="NYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTKESAVILNKLKQ
                     IVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYSMMFGEVDKKFF
                     MKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG"
BASE COUNT          974 a          874 c          608 g         1075 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg g
//
LOCUS       DACCQP010000148         3395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCQP010000148 DACCQP010000000
VERSION     DACCQP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.156"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(263..1210)
                     /locus_tag="IVO87_002837"
     CDS             complement(263..1210)
                     /locus_tag="IVO87_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7450957.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1215..1967)
                     /locus_tag="IVO87_002838"
     CDS             complement(1215..1967)
                     /locus_tag="IVO87_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7450958.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1968..2876)
                     /locus_tag="IVO87_002839"
     CDS             complement(1968..2876)
                     /locus_tag="IVO87_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7450959.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2876..>3395)
                     /locus_tag="IVO87_002840"
     CDS             complement(2876..>3395)
                     /locus_tag="IVO87_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7450960.1"
                     /translation="WEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQK
                     LSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLF
                     SWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDK
                     YIINLINDIEVN"
BASE COUNT         1115 a          644 c          416 g         1220 t
ORIGIN      
        1 tctatggggc gcttacctta cttcgcaact tttacaattt caaactacga attatgattc
       61 gtcatggcaa aacattttta accaaataaa cagaacagaa agaggttttc ttctttctgt
      121 tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag agcccttcta
      181 tcccagattt gattttacaa aactcatcag tccttattga caaagagcct gaatttaccc
      241 tctaatctta tcttagtatg ttttaattga tttcagtttt caaataatta gtcattccac
      301 ttaaaatacc atatttcggt tgatagccaa ttgactggat cttagtaata tctgctagag
      361 agtcatggat atcacccgaa cgctcttctt tgtattcaag tgtcaattct acaccgagaa
      421 tttcattgat tgcatgaatc aattccaaaa gtgtggttgc tttacccgtt ccaacattga
      481 attgtttccc cagtgcagct tcctcattcg caactaaaag taatgcttgg attacatcat
      541 ctatataaac aaagtctcta gactggcttc catcaccaaa taatgtaaat gaagttgctt
      601 ctcctacgat ttgtttctta tagcgatcta cgagaataga aatcacacca ctgtatggag
      661 aattcggatt ttgatttgga ccatagacat tgaaaaaacg aactgcgcta ccgggtacat
      721 catataaatg acaataatct agcacatatt gttctgcagc gaatttatca accgcataag
      781 gcgtcaatgg gcgaatcacg gactcttctt tttttggtaa agtcggttca tccccataaa
      841 cagccgctga agaactaaac acgatccgtt ttaaatcagg ttgatacttg cggatcaatt
      901 ctaaaatcat taatacactt tcaaaattca cacgatgtgt ttctactggt cgggcaactg
      961 aatctgccac actagcaact gctgctaaat gaaagatata atcaaattta ttagctttca
     1021 tgatctcttc catcaaggat ttctcagcga catctccctc aataaaagtg atatttttcg
     1081 tcatatcaag attttctact tttcccattg ataagtcatc gacgacgatt accacatgtt
     1141 cttctcctaa atgattggct aaggtagaac caatgaagcc agccccaccg gtgataagat
     1201 aacagctcat ccttttacct ccgatataaa taattataga tatcttgaag ctccgaagaa
     1261 tcttctaaaa atttaggaaa caaaaattct aaccatttaa aatcttcttc actatcaata
     1321 tctaaaatta gataatctct catttttatt atcccacatt gcccatcaaa aatataatta
     1381 ctttttttta gaaattcagg tttaaaagaa tacattgagg cattcatttc aaaaacttca
     1441 ggtgtctgtt gcctagatgt gtatcttgat ttattaacta tagtaacttc attttcaatt
     1501 ctttttacca tattaaaata gggattacgt cttgcagaaa caactgaaaa gtagacgtct
     1561 ttatctaaat tttttagatg ttcatctatt aattcttcaa gattagaaac ttcacgtaaa
     1621 ggtgaagtca aatctaaatc tataattaaa tcaaatatat tatcttctct ttctagttgt
     1681 aagaatgaat cacgtatcac ttcaattttc cctacagtat ctgtagctaa ttcaggttta
     1741 cgttctacaa ttttcacaga cggatttaac ttagtcgtca attctactaa ctcattactg
     1801 tccgtattca aacaagtcgt gcaattatgt tcaggatgtt tttgttgata gatatctatc
     1861 attgctaaag cgaaatggac taggggcttt cctgccaaat atttcaaatt tttattttta
     1921 aaccccttag acccagctct accacaaata gtaaataaaa tttccattta tttctcctca
     1981 gctattttca atacttcgtt tgcatgatat aaatcattcc agttttcttt gcctgttact
     2041 acgttttcaa agaaatattt caactccttc tcatacatat cattagcaga ttcaggcatt
     2101 tctttaacaa tgttttcttc aaaacttatt ttattattta ataagtcaac attgatacat
     2161 tcatctaaag taaacaattg aaattttctt gtaggaactc ttccaaaata atctaaatga
     2221 atttctataa ttttatcatc gtatctcaat atatatgttg ctaaatcatt actgttaatt
     2281 tcaagattcg aatatttacc aaactgtttt tttactgatt tagggaaacc aaataggttt
     2341 ataatataat ccaattcatg tatacagtct aattctactc ctccccccat agatttaaag
     2401 gcagaataat tatttctata atcagtacct tttctccaat caggaagata agtagaacaa
     2461 atagctctta cagaatatac ttttttactt tgaatactat tacgaacaac ttccatgata
     2521 tttttatacc tcagtggagc agcaacataa atatttttgt aatcataatt atattgagta
     2581 agatcaatac tctcagaaaa agcaggtttt tctaaaaata tgtaatcaac tttttcaaca
     2641 attacattta gtgtttccag atgcttaaaa gtcggatttg taataaaagc tatatcataa
     2701 tgtgtatcca aatcgtctct attttgaata tacgttattt cttcaaactc tttttttcca
     2761 ctgctactat aagctgttat atcaaaatta atagaattct tttttccata gcttattaga
     2821 ttttttagat gtctctttcc aattgaccct aacccaaaaa aaattacttt catcattaat
     2881 ttacctcaat gtcgttaatt aagttaataa tatacttatc tttttcaaaa gtatattggg
     2941 gttccatttt cttttccaaa agcgtgaaaa attctttaat ctcctctaaa tatgcatctt
     3001 caataatagt atgtgaataa ttattatcat gcataacctc atcgtacaat tgaatatctg
     3061 tcatactaga agtttcttca ttccatgaaa atagagaatc cgggttacct tcccattcta
     3121 ttaaaatctt ttcaccaatt atagttaaat ttcttttagc tactcttgaa acaacatcta
     3181 cacttaaagt tccaactata ccagtttcat gctcaataat aagagtgaaa ctgtccgaat
     3241 aatcaatatc aagattggat aatttctggg tcattacagt aaacgattta actttcccaa
     3301 atgtatctat aatccaaggt aattcaatag cgaatatttc tctacaccca ttagtttttt
     3361 tgtttgaaac aaaaaaattg cgataatctt cccaa
//
LOCUS       DACCQP010000149         3382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCQP010000149 DACCQP010000000
VERSION     DACCQP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.157"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..246
                     /locus_tag="IVO87_002841"
     CDS             <1..246
                     /locus_tag="IVO87_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450961.1"
                     /translation="EKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDNCCSKYKVNQVRE
                     ALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            349..663
                     /locus_tag="IVO87_002842"
     CDS             349..663
                     /locus_tag="IVO87_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7450962.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            641..2335
                     /locus_tag="IVO87_002843"
     CDS             641..2335
                     /locus_tag="IVO87_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7450963.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2355..>3382
                     /locus_tag="IVO87_002844"
     CDS             2355..>3382
                     /locus_tag="IVO87_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7450964.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         1212 a          467 c          686 g         1017 t
ORIGIN      
        1 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
       61 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
      121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
      181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
      241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
      301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
      361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
      421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
      481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
      541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
      601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
      661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
      721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
      781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
      841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
      901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
      961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     1021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     1081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     1141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     1201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     1261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     1321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     1381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     1441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     1501 aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     1561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     1621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     1681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     1741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     1801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     1861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     1921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     1981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     2041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     2101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     2161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     2221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     2281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     2341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     2401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     2461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     2521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     2581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     2641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     2701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     2761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     2821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     2881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     2941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     3001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     3061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     3121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     3181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     3241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     3301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     3361 taggcattag aaatggagta tt
//
LOCUS       DACCQP010000150         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCQP010000150 DACCQP010000000
VERSION     DACCQP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.158"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IVO87_002845"
     CDS             301..585
                     /locus_tag="IVO87_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450965.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IVO87_002846"
     CDS             588..1004
                     /locus_tag="IVO87_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450966.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IVO87_002847"
     CDS             complement(1141..1860)
                     /locus_tag="IVO87_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7450967.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IVO87_002848"
     CDS             2513..2608
                     /locus_tag="IVO87_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7450968.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IVO87_002849"
     CDS             complement(2910..3155)
                     /locus_tag="IVO87_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450969.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1064 a          598 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atccaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACCQP010000151         3161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCQP010000151 DACCQP010000000
VERSION     DACCQP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.159"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1538
                     /gene="fss3"
                     /locus_tag="IVO87_002850"
     CDS             <1..1538
                     /gene="fss3"
                     /locus_tag="IVO87_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7450970.1"
                     /translation="APEGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFD
                     FFKFAGSAAGTTETGFNDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENL
                     PYGDYLLEEVEAPEGFQKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTV
                     PYEKLTNKAFSVSLNRLMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNL
                     HEIKEDWYVVAQAIDVDATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTA
                     EQVLNKTIVLFNYVYENKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLE
                     NGSQTFTHGDVVDMFDDVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEV
                     NDKEFTKTVLAKKVDTGKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEV
                     PTILSTPKQPETPTVPSGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWN
                     RRN"
     gene            1651..1965
                     /locus_tag="IVO87_002851"
     CDS             1651..1965
                     /locus_tag="IVO87_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7450971.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            1978..2352
                     /locus_tag="IVO87_002852"
     CDS             1978..2352
                     /locus_tag="IVO87_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7450972.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            2362..2697
                     /locus_tag="IVO87_002853"
     CDS             2362..2697
                     /locus_tag="IVO87_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7450973.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            2780..>3161
                     /locus_tag="IVO87_002854"
     CDS             2780..>3161
                     /locus_tag="IVO87_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450974.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRET"
BASE COUNT         1061 a          531 c          713 g          856 t
ORIGIN      
        1 aagcgcccga aggatatagc cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg
       61 atgatagcga aaaaaatgcc gtgattaccc gaaatgttac ggcaaaagaa caagtgattc
      121 gctttggctt tgatttcttt aaatttgctg gatcagctgc gggcactacc gaaactggct
      181 tcaatgatct gacctttaaa gtgtcgccat tggaagggac caatgaaatc acaggtgccg
      241 aagatgaagc gaccacagct tataacgagc aattagggtt tgatggttat ggcaagtttg
      301 aaaatcttcc ttatggggat tatttacttg aagaagtaga agccccagaa ggttttcaaa
      361 agattacgcc attagaaatc cgttctactt ttaaggaaaa caaagaagac tttgtgaaga
      421 gtgagtatgt ctttaccatt accgaacaag accaaaaaca accaattaaa acggtgaccg
      481 ttccttacga gaagctgacg aacaaagcat tttctgttag tttgaaccgt ttaatgctct
      541 atgatttgcc tgaggaagaa gatagtttaa cttctcttgc gacttggaaa gacggaaaca
      601 aagaattaac ttctctggat tctaccgagc tagttgataa attgagttac aatctacatg
      661 aaatcaaaga agactggtat gtggtagcgc aagccattga tgtggatgcg acaaaagctg
      721 cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc agaaacaagc gcaacgttgg
      781 cgaacaaaga gaaaacagga acttggaaaa ttcagcataa attaaccgcc gaacaagttt
      841 taaataaaac tattgtctta ttcaactatg tgtatgaaaa taaggaagct tttgaagcag
      901 gcgataagcc agtagcgaag gatgttagct tgaataacca agcccaaact gtcagttgta
      961 cagtggaaca ccatgtttct atccaaacaa aggcccacct agaaaacggt tctcagacct
     1021 ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc gattactcat gatgtactgg
     1081 atggctcaaa agaagctttc gaaacaattc tgtatgcttt attaccggat ggtacaaaca
     1141 aagaaatttg gaaatctggc aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa
     1201 ctgtgcttgc gaaaaaagta gataccggaa aatatccaga aggaaccaag tttacttttg
     1261 cggaaatcaa ttacgataaa gatgggaata tcaatggaaa acacaatgaa gatttgaaag
     1321 aaaaatctca aaccttaaca ccaaaagaag ttccaaccat actaagtacg ccgaaacaac
     1381 cggaaacacc aactgttcca agtggctctc aagaatctag tcccacagtg aagaccttcc
     1441 ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat tggttttact ttgatttttg
     1501 cgacggcggg ctattatttc tggaatcgcc ggaactaagg tgatgagatg atacaagtaa
     1561 atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc
     1621 caaaactaac aatgaaaaga ggaaaagaag atggaattga aatttgtcgt gccagatatg
     1681 gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag aaattataac agaaggatat
     1741 ggacggaata ctacggtgat tggtcgcagt tatcatttgt attccagtaa gcaacgggcc
     1801 gatgatattg aggtggtagt agctgcggaa gccggtgaaa aggattttga tcaagaccaa
     1861 ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg
     1921 tttactgatt acgtgttgta tgttgatgat ttagtgaaag cataggagga aaagaagatg
     1981 agattaacag aaggtattgt cgtagattca ggtttaacat ttgggaagtt gcgtttttcc
     2041 gcattacgcc gagaagtacg gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa
     2101 gaacgaacct ataatttaaa atcctctgca caagggcgaa tgattcaagt tagtatccca
     2161 gctaatgtac ccttacgcga gtttgcttat gatgcagagg tagagttggt caatccaatc
     2221 gtggataccg ttgccaatta tgtttttcga gaaggaacta ccgttaattg gttcattaag
     2281 gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa
     2341 agaaagaaat aggtgaagat tatggaaatt aatgttgaac atattttaga ttgtctcgat
     2401 caatatggaa aaggcgagct gactgaggaa caactcgtta aagcattaac ctctgatgaa
     2461 aaaatgtttc tgattataca tcaaggttta cttggagaaa atagcgacag tgaagaagac
     2521 tctgatgtac tagactggtt aggagaagaa gagtctttct ttatagttgt cgaagtaaat
     2581 gaaaacttat gtcggcaggc agaatccatt ttggaagaaa ttggtgtgga gatgccggat
     2641 gctattgaag gctttttaaa tcaattggtt gaaacgaaac aactcccctc ctcgtagcag
     2701 taaatgacta gcataacttt ttgaaattgc ctaatgaaat cctattgggc tttttcgatt
     2761 ggaggtggaa accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcagcactt
     2821 ggtttatcac tttgttcttg gctggctact cgctctgttc atcggttgga tgagcgtctt
     2881 ttattttcaa gagtttagac aatttgatat ttctaagcta tcactttcta ctattgaaat
     2941 cgtttggtct ataaaagatt tagtatgtct gcttggaagt ctcgcttttt caggagctat
     3001 gatattgctt tatattcact tttttcttga tcattggcga agcttgtggc atcgacaaaa
     3061 gctagctcgg atgattctag agaaccattg gtatgaagtg aaacaaactc aaagtgaagg
     3121 ctttttcaaa gatttgaata gtagccgaac cagagagact a
//
LOCUS       DACCQP010000152         2969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCQP010000152 DACCQP010000000
VERSION     DACCQP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.160"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..620)
                     /locus_tag="IVO87_002855"
     CDS             complement(<1..620)
                     /locus_tag="IVO87_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450975.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPK"
     gene            complement(1079..1336)
                     /locus_tag="IVO87_002856"
     CDS             complement(1079..1336)
                     /locus_tag="IVO87_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7450976.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1329..1598)
                     /locus_tag="IVO87_002857"
     CDS             complement(1329..1598)
                     /locus_tag="IVO87_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7450977.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1729..1908)
                     /locus_tag="IVO87_002858"
     CDS             complement(1729..1908)
                     /locus_tag="IVO87_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450978.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2289..>2969)
                     /locus_tag="IVO87_002859"
     CDS             complement(2289..>2969)
                     /locus_tag="IVO87_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450979.1"
                     /translation="TEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYL
                     ESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSY
                     TICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLM
                     GMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQ
                     RFERKKRQ"
BASE COUNT          897 a          565 c          397 g         1110 t
ORIGIN      
        1 tctttcggaa actcaatatt aaagtaccct ttaaacatgc tccctaataa tttgtctaca
       61 gggggagatt gtaaattctc aaatattaga gttaaatcag gtttctcttg ttgataaaat
      121 tcctttaccc ctatttcaca ggctgtaatt atcgaaataa acctatctct agtatttgat
      181 atatttttac ttcttttaag gagtataaga gaaaagggga tactttcgtg tccatgcatg
      241 taattaacta attgttgagc ttcagaagtt atatctctaa ttatatagtt aacggaatta
      301 ggaagtgtct ttaactctaa tgaaatttct atccaattac tatcttctaa tctgtatatg
      361 ctcgaagtat aatttgattc gtcagcaagc atcgctacat cccaaacact gatcataaaa
      421 tcttttaaac gttcaatcat ttcagcaata ttgctaaaat cagctaattt ttctttagaa
      481 taaaaaaaaa catttatatc agaatcaata aattctattt tttttattac tatatcgtca
      541 tcattatctt gaccatttat gtttattgtt ccctgagtgg ggagtacttc tttcaaattc
      601 aagttaaatt tatattccat tacttctcct ccaatatata aaaattactt aactgtatcc
      661 aaatttaata tgtaaataaa tcattaattt ttagctaaat tctatgagaa gtttattttt
      721 gttgtttttg ctaattaact aaaactagta caaaaaattt aaaatagatt attaaaccta
      781 ttgcttattc tattcagtca tatatataag cgtcatcctt tagtaactaa catcccacac
      841 tacaatagta ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata
      901 tttacttttc ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac
      961 aaacacattc attaataaca atcaacacta agctatatta gctttttaaa tgactagggt
     1021 aaataaaata aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt
     1081 aatagtgatc ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc
     1141 tatgttcatc tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag
     1201 gttttcctaa tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt
     1261 ttatattgct tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag
     1321 accaagcctt aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg
     1381 gagaattgtt catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc
     1441 aacgtttctt gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca
     1501 tctttacttg ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt
     1561 aaattttggc ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt
     1621 ctataaaaca attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct
     1681 tataattaaa agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag
     1741 tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat
     1801 aatcctggat tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt
     1861 ttcatccaac gtaacgggta accgtttatt tcctattgca attggcatta gtactctctc
     1921 ctttcatcga aataacccta tgaaaaatat ggctgcaatc atcttgaata gtagactcac
     1981 aagggctaat acaaagcgcc aaaagatcaa acaaaatacc agtattgcca aaaatactac
     2041 gaagtttaaa tgcacagctg cagcatggcg cataagtatt tcgccaacaa ataggactaa
     2101 caatagagga atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggtgtt
     2161 gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa
     2221 gaaccattcc cttagttact aggttttttg tccttatttc acacattctc acttttcggt
     2281 ataaatgatt attgcctttt tttcctttca aatcgttgat atggcattcg tttacggtca
     2341 agttcatttt ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa
     2401 gtcgtgtacc agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct
     2461 tcgggtacag cgagttcata atccattccc attaacacga tagattccgt tccacacgtc
     2521 aaaaatctag taaatacaag gtaataatcg tctatcgtcg catctttgct gtttagctct
     2581 tccatttctt cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag
     2641 taatagtgtt tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct
     2701 aggtaaacat tcatgatgta tttttctgtt tctagtcgta gaagctccgt tccttttttt
     2761 accgtttcag gacgacgttt atacacgtct aaacgattat ggctgactgt gaccttcgtt
     2821 tgactcgttt cactttccaa atacaattgt tcttcgactc tttgaataaa gtacttcatt
     2881 tcttccatac aactaaagtc tttttgaatc cgtctgatta cttcattcaa atgttctttg
     2941 tttgttaaaa agtgtgacac atcttccgt
//
LOCUS       DACCQP010000153         2954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCQP010000153 DACCQP010000000
VERSION     DACCQP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.161"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            89..406
                     /locus_tag="IVO87_002860"
     CDS             89..406
                     /locus_tag="IVO87_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7450980.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="IVO87_002861"
     CDS             complement(497..862)
                     /locus_tag="IVO87_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450981.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="IVO87_002862"
     CDS             1070..1552
                     /locus_tag="IVO87_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450982.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /gene="pstS"
                     /locus_tag="IVO87_002863"
     CDS             1749..2639
                     /gene="pstS"
                     /locus_tag="IVO87_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7450983.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1009 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tgta
//
LOCUS       DACCQP010000154         2886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCQP010000154 DACCQP010000000
VERSION     DACCQP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.162"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IVO87_002864"
     CDS             287..694
                     /locus_tag="IVO87_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450984.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVO87_002865"
     CDS             708..1109
                     /locus_tag="IVO87_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450985.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVO87_002866"
     CDS             1111..1482
                     /locus_tag="IVO87_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450986.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVO87_002867"
     CDS             complement(1518..1820)
                     /locus_tag="IVO87_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450987.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="IVO87_002868"
     CDS             complement(1916..2710)
                     /locus_tag="IVO87_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP7450988.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1022 a          411 c          439 g         1014 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataatt
//
LOCUS       DACCQP010000155         2820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCQP010000155 DACCQP010000000
VERSION     DACCQP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.163"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1648)
                     /locus_tag="IVO87_002869"
     CDS             complement(<1..1648)
                     /locus_tag="IVO87_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450989.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKV"
     gene            complement(1672..>2820)
                     /locus_tag="IVO87_002870"
     CDS             complement(1672..>2820)
                     /locus_tag="IVO87_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450990.1"
                     /translation="GGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTP
                     DKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLI
                     SDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQ
                     GNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRT
                     MKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLL
                     NAYETPEYYFADYGEAVTGDDGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHE
                     DIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          809 a          599 c          440 g          972 t
ORIGIN      
        1 atactttccc atcggtataa ttgtttgctt taccagtaat ttcagaaatt ttaatatctg
       61 tggccaaatt atcttcgaca tattctggtg ctagatccca aacataatct tttggattgt
      121 ttgaatcacg cataccagta ccacgatatt tatactcact aatattcggg gttcgagtat
      181 tgccttcctc tagttttaac cacttaattg tacattgtcc tacacttgtg cttggtactt
      241 gataaatctg cacttgtggg gtagtagggt gtgagtcatc tgctgctgta aatgttttag
      301 accacacatt agttaagcct tctactggtt gtaagtcacc aacctcccat gcatctcctg
      361 ttgctcgtgt gaaaaatggt ctaaaaacct gtgttgctgg cttagttcct tcaagtgtga
      421 tggtatactt cttacctttt accattggtt taatgttata ggtgttaata agatagttac
      481 tattagtaac tggttgttgt gattctggtt taattagatt ctcacccaaa gccaccttac
      541 tcaaataata cggtgcatca agtagattag gctggtacgg tgtggctgtt gagccttctt
      601 cgattttgat gtcgtacaac ttaaatccgc catttatttt atccctgtca acaaagctaa
      661 tactcatata aaatcgatct aaatttgtga tttgataatt aacgttagct gtacctttga
      721 ttgttatttc tttccctaca tcatctgttg taatatttgt actatttgct tccaataata
      781 tcttttctcc tggtgatgta cgatacacca aacgtaattt atcaatagct cctgtagttc
      841 cttcatcaaa tcgaactttc gcactcagag tataggtttt cccactagta agctgaggtg
      901 tattaatacg cgtaaacata ataatgcttc ctgtaccatc agaagtaaaa tgtaactttt
      961 cgttatccaa agttagtgac ccgtggtaac caacgttaaa atcgctcgat tttagtttgg
     1021 acattaaatt cggcttaccg ctataatcat atcccccgaa gtcgatgctg ttactgtaca
     1081 tcacttgtaa gttacctaac ttagaaattt cttctttcag agcatctaac ttgtcttgta
     1141 gcgttttagc ttgaccagtt aaatcagtaa tctgttgatt taagctatcc actctacctt
     1201 tgatttcagc cataaaagca tcaaaagttt cgttgtactt tcgaatcaac tcttctaatt
     1261 gcgaaacata ttcatcggct tggccttgcg aaatgtcaga cactcctagt gagaaaaaaa
     1321 tgatatcttg cgttgttaaa atttgattgt cttttctata ttctacgtag cagtgtttat
     1381 aatatcctgc ttcactcata aatgtgccat caagagaaaa cgtgacttct tcactagtta
     1441 cactagttgc aacattatct acgtaacggt tagatggtgt tgttcctttt aaagtaaatg
     1501 ttccgccact cgtatccatc tgcaagccat ttagaaacgg tttaaccgtc accgtaatcc
     1561 ctttatcacc ttgacgagcc ataatagctt tggtgtagtt taattctttg ctgaaatcta
     1621 aagccaaatt atataaactg ctagccattt atatacctcc ttgtcttcgt tttaaaaacg
     1681 tttttggtca agcactgtgc tatcatatgc tgtatcctct tttaatctaa tatcttcata
     1741 ccctagacgg tgtgccacta aattccatct aactaatacg tttggcttac tagtttcaat
     1801 gatgaaatgg tcaatatctt catgagtaac agcacacaaa actagttctg taggtgtcac
     1861 atgtgtcata taccgactta gatttactgt ttcagcaaac atggggtcaa tatcaacacg
     1921 aactttacca tcgtcacctg taacggcttc cccataatca gcgaaataat attctggagt
     1981 ttcataagcg ttcaatagtc gttgtccata atgttctgtt ggtacagttg agtttttagt
     2041 acctctaaca gtaaaatctt tatatacttg taccgttgat tgttcaaacc tagcaagttt
     2101 cccatcttcc catgaaccaa aaaaacaacc tggtaacgtt agcataccat cactagtaaa
     2161 tttcatagtc ctaccagcta ccttaaattc ccatgagtta cccgcactac cattaatgct
     2221 taaagaacta ccgtcgccag aagttacata actagcattg ctatacctga aattgggcgc
     2281 accaaaagat agaaacggtc tgttattacc attatcccac gtactaaaaa ccaagttacc
     2341 ctgtggattt ctaatcatga aaccaccacc agttttcatg gtgtatgata caataccggc
     2401 atcagcactt acataatcac gtgcttctag ctccataata tctttgttaa ctttttttga
     2461 gtaccaagtc attttgccat tagcaatact tgttctataa tcagcaccat cactaattaa
     2521 tgtagtacct ctaatagtaa ttcctactat ttcaccagcc gtaataaacg aggcattgaa
     2581 tccgccatct aacgtccatg ccgtttcata tgttccatta atgccagttt tagaaaaacc
     2641 aataccagca ttgttgattt gtaaaacatt ccttgcggta ttcttatctg gtgtgtccat
     2701 aatcaaaata cgactaggcg cttctttagg atctaataaa acataaccac cattttgacc
     2761 agtaatcata tcagtttgat gatctacaat atcattgagt aaatcactga tttcgccacc
//
LOCUS       DACCQP010000156         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCQP010000156 DACCQP010000000
VERSION     DACCQP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.164"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVO87_002871"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVO87_002871"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7450991.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACCQP010000157         2607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCQP010000157 DACCQP010000000
VERSION     DACCQP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.165"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1166
                     /locus_tag="IVO87_002872"
     CDS             <1..1166
                     /locus_tag="IVO87_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7450992.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGI
                     KNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKY
                     NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDG
                     SKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADK
                     NGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGS
                     NSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1300..>2607
                     /locus_tag="IVO87_002873"
     CDS             1300..>2607
                     /locus_tag="IVO87_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7450993.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1025 a          345 c          502 g          735 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggcaattatg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct aatgcagata
      241 aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca gatgcatctg
      301 agggaattaa gaacaaagaa gatttaccaa aaggaactac ttatgcttgg aaagagaaag
      361 tagatgttag cacagctgga aataaaaaag gtactattgt agtgacatat ccagatggat
      421 catctgatga agttgaagta gacgttacag tgacagatac tcgttccgat gcagataaat
      481 ataatcctga aggacaaaaa gtgactacag acttaaataa agagccagat gcatctgagg
      541 gaattaagaa caaagaagat ctaccaaaag gaactactta tacttggaaa gaaaaagtag
      601 atgttagtac agctggaaac aaaaaaggta ctgtagtagt aacatatcca gatggatcta
      661 aagaagaagt agaagttact atttctgtag tagataaaaa agcaccgaat aaacctcaag
      721 ttgatcctat tacagagggt gaccaaattg ttactggtaa aactgaacca aatgcagagg
      781 tgacagttac attaccagac ggaagtcaac accatgggac agctgataaa aatggtaatt
      841 ttacagttaa agttcctaaa ttagaggcag ggacaaaagt gatagtaact gcaactgatg
      901 aatctggtaa tactagtgaa cctactaatg tagttgtttc ttccaacaaa aaagacagtg
      961 gaaaaaacgg tagtaaaggt agtaagactg acaatcaagg tagcaacagc aatcaagaca
     1021 aaaatagagg caaaagccag tcctcaaaag tttttcctaa gacgggagag agtgacagta
     1081 atatctttac tattagcggt ggtttaatac tgttaggaac gttaggatta ttagggtata
     1141 aaaatcgaaa aaaagagaat gaatagaaaa tcaatttctt aagtcttctc tgagaagagg
     1201 aagaatcttt ttcttcctct ttttgctttt ttatgatggg aaataagtgt tagttaaaga
     1261 tgaatatact ttttatagaa aggaaaagaa tgtaattaag tggaaaaaat agtaattatt
     1321 ggttcaaacc attcagggct agccgcagct cgatttctaa acttaagcga ttcaaattat
     1381 gaagttattc taatcgataa aaaatcagaa attagttact tggaatgtgg gacatcgtta
     1441 tttataaaag gagaagtaag taatttagaa gatctttttt atcaaaatag aatgacacta
     1501 aattctgaga taaaaaagtt atttctagag acagaagttg acaaaattga tattcaaaat
     1561 aaaaagatta gttgtgtaaa aaaagatggt acaagattct ctcaagaata tgacaaagtt
     1621 atattagcta caggttcaaa gcaaattatg ctagatatac cggggaataa tttagctaat
     1681 attttttcga taaagaattt tccaaatgca aaagagattt ttaaagcttt ggatgatagt
     1741 actattaaaa aagtaggaat tattggagca ggatatattg gagttgaaat tgctgaagcc
     1801 ataagaaaaa gagggaaaga agtatatctt ttcgatgttg cagatagagt gttgtcgact
     1861 tattacgacc gtagtttttc agataaagtt gaagaaatac tatcaaaaaa tggaatacat
     1921 ctttgtctat cagaaagtcc taaaaaatat ttaggaagaa aaaagattga aaaaatagtc
     1981 actacaagtg gtacccaata ctctatggat atggtcgtac aagctatagg atttctatct
     2041 aatagtccaa ttggggaaaa ggaactcttg agagatgtta gtggagcata tctaactaat
     2101 gaataccagc aaacaaatat aaaagatata tatgctattg gggattgtgc tacaactttt
     2161 tttacgtcca ttaatagaag tagtgttgat ttttctattt ctaatgctct aagaacagct
     2221 tatattgcaa ctcaacatat taacaatcaa gattttacac cttctggttc ccaatttaca
     2281 aatggctttt ctatatttaa ttacaatttt tacgcagcag gtttgaattt aaaaacagca
     2341 cgattattaa gtgagtctat agattatata gaaatagaag atttcattag acctgctttt
     2401 ttgagaaaaa atagtcgtgt aaaattaaga attagctttg aaaaaaatac aaagagaata
     2461 gtaggtgtac aactttgtag tcaagaagat ttatctggac tattatcaat gttttctctt
     2521 gctatagaag aacaaattac attagacaaa ttgaaaatgt tagattattt atttcatcct
     2581 agttttagcc aaccctataa ttttatt
//
LOCUS       DACCQP010000158         2593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCQP010000158 DACCQP010000000
VERSION     DACCQP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.166"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1152
                     /locus_tag="IVO87_002874"
     CDS             <1..1152
                     /locus_tag="IVO87_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450994.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IVO87_002875"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IVO87_002875"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7450995.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          859 a          491 c          620 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca act
//
LOCUS       DACCQP010000159         2570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCQP010000159 DACCQP010000000
VERSION     DACCQP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.167"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..549
                     /locus_tag="IVO87_002876"
     CDS             <1..549
                     /locus_tag="IVO87_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450996.1"
                     /translation="IEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR
                     FEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKC
                     ITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFK
                     NKYQIKGIKLNQKTIKKLNIGG"
     gene            552..1301
                     /locus_tag="IVO87_002877"
     CDS             552..1301
                     /locus_tag="IVO87_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7450997.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1313..1582
                     /locus_tag="IVO87_002878"
     CDS             1313..1582
                     /locus_tag="IVO87_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450998.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1744..2046
                     /locus_tag="IVO87_002879"
     CDS             1744..2046
                     /locus_tag="IVO87_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7450999.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2043..2204
                     /locus_tag="IVO87_002880"
     CDS             2043..2204
                     /locus_tag="IVO87_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7451000.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2201..2506
                     /locus_tag="IVO87_002881"
     CDS             2201..2506
                     /locus_tag="IVO87_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451001.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          980 a          424 c          571 g          595 t
ORIGIN      
        1 attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa cttcatctat
       61 gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg gaaggaattc
      121 ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga cgaatcgaat
      181 acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat ctttgaacat
      241 catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata ttacttctac
      301 aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga catcgatcac
      361 tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga gtacacattt
      421 gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat taatttcaaa
      481 aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt
      541 ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc aacgtaaggt
      601 acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg gccttgtgta
      661 atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat ggtgtgaata
      721 aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg aatcgggaaa
      781 tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt aatcaatacc
      841 ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat aatacatcta
      901 ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct tcgaaaaaat
      961 cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt ttgtcagatg
     1021 agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga atcgaacgac
     1081 tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca actattcgaa
     1141 gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac aagcgcacag
     1201 gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc aagaaaaaag
     1261 cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa aaatgaagat
     1321 cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg aattagacgt
     1381 cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta agaatgatcg
     1441 ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa cacaattaag
     1501 aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc aaaaacatct
     1561 taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca cagattgaac
     1621 taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg ctcaacacgt
     1681 tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa ggagaatgtc
     1741 gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc agaagattgg
     1801 caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat gtgggcagac
     1861 aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa acttctagtg
     1921 atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat cgtgaggaaa
     1981 agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt ggaggaatca
     2041 tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt gagattgacg
     2101 atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca atcgatttgc
     2161 acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg ataagaaagc
     2221 agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag acaaagaaat
     2281 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     2341 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
     2401 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
     2461 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
     2521 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
//
LOCUS       DACCQP010000160         2520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCQP010000160 DACCQP010000000
VERSION     DACCQP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.168"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IVO87_002882"
     CDS             5..1150
                     /locus_tag="IVO87_002882"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7451002.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVO87_002883"
     CDS             1321..1779
                     /locus_tag="IVO87_002883"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7451003.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVO87_002884"
     CDS             1938..2408
                     /locus_tag="IVO87_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7451004.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          837 a          482 c          474 g          727 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
//
LOCUS       DACCQP010000161         2507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCQP010000161 DACCQP010000000
VERSION     DACCQP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.169"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(3..233)
                     /locus_tag="IVO87_002885"
     CDS             complement(3..233)
                     /locus_tag="IVO87_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451005.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            850..1512
                     /locus_tag="IVO87_002886"
     CDS             850..1512
                     /locus_tag="IVO87_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451006.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1591..>2507
                     /gene="ecbA"
                     /locus_tag="IVO87_002887"
     CDS             1591..>2507
                     /gene="ecbA"
                     /locus_tag="IVO87_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HAP7451007.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKT"
BASE COUNT          877 a          404 c          474 g          752 t
ORIGIN      
        1 cattactccg atctgttttt aaaattagtt tctacctcta atatatatcg agtttttatt
       61 ttgccttcag agaatactgt ttcttttttt gcagttacag gttgtttatt ttcggaaaaa
      121 gctaatatag ctaagattga aacatccatc tggaatttat cttttttact gctttgatca
      181 taaaagtctg catattcatc actgatattt tttctaataa attcttccat cataaaaatc
      241 acctcaaaac gattatacga acttacgttc ttgttgtaaa gcgatatttg aggggcaaaa
      301 agggggcaaa aatgcgttta cctttgatat atctcagatt gaacatagtg tttattttat
      361 gtcggtattg ttgatttata gctgttttga gggtgttaga tacataatag aatatagtgg
      421 ttttgtttac gtgcattaca cggaaaagct gctcgtatca aagaaatcag aagataaaca
      481 cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt
      541 cttttttctc tatcacagtt ttaagccatt tttggctgtt ttggaccatt tttcaatcat
      601 attcccttaa aaattgtcct agattgtccg acattgacta tcagtgtcgt caaaataggg
      661 caaaatttag tagtcagctt gacgacaaat ataatacgaa aaataccaaa acatttaccg
      721 aatttagcaa cttttgaacc caagtttctc aaagggtcag ctattagcaa aaaagacaaa
      781 acatgtactt ttctactaaa aagttgatgt gataggcttc tatctaaata actattagga
      841 gtttttatcg tgaagcaaaa tgatggacgt atcatttgga aaaatcaaag tgagttaaaa
      901 ctgattttaa caattaatga attcatcgaa aaacacggaa ttacatctag ccgtcaatac
      961 cagaaaaagc tatcagaaaa tccaaattct gcaccaagta tgtggttcat taacaaaaaa
     1021 tatggatcat gggagaattt attgattagt ataggacgag aaaatactgg gtatggaaaa
     1081 tgggctagaa tgtcagaaca agaattattg gaaatagtag aagcctttat taagtgcgag
     1141 aagataacct ctcaacgaat gtacgaacaa aaatcagtag ggaagaatat tccttcactt
     1201 agtaccatta aaaagatgtt aggagatata cgtccacttt ttaaagaaaa aaatgatgga
     1261 tctcgattta ctgatttcga actgctgtta gaactaaaaa atgaaataat ccgattaaaa
     1321 ctacaggatg atttatcaat gacaaagttt cgaaaactag ttcaatcccc aaaactacca
     1381 tcagttgata caattatgaa aagaacgaac aaaaattggg aggaattgat ggcagagatt
     1441 ggatttgatt atcgaagaat caaaatttat aagcagagaa acaacctatc caagacaaag
     1501 aaaactaaat agtatcactc taaatcagac gttcaaaatt aaaatttgga cgtctttttt
     1561 attacccaaa aacgaaagga gatttatttt atgaaaaagc caagatttaa gaattggcgt
     1621 ttgctcgcaa cactgagtct attatgccaa acaattggtg gatcgcttgg cccaacgatt
     1681 gcctttgctg atgaaattac tcatccacaa acggtaactg tggaattgga tttggctcac
     1741 cagtatgctg tcgaaggtac atttagcgat ggtcgtccca tgtcagaagt taccgttccg
     1801 cactatgcag tttacaatgg tgtgaagcaa gatattttct gtattgagcc aggggtgccg
     1861 atttacaatg agttcactcc tggttatgag aaaaatccat tgcctgatat gtcggagaaa
     1921 gcgaaattag tttccgtttt atggaaggaa gctggtacgg atgtggatac acatattgtg
     1981 gcccagaaga tgatttggca agaagttaat ggctacacgc ttcactcaat taaacgttct
     2041 gatggtagtg cagtaaatat cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat
     2101 tatcaaaaga aaccaagctt ccataactcc acagtgaaaa ccgtgttagg tcaatcgact
     2161 actgtgacag atacgaacgg cttgaattta tcggagtttg atgaagttgt cgagaatatc
     2221 gcaaatattg attatcgtgt gaacggcaat caactagtga ttactccaaa tgccaattca
     2281 aaagagagtg gtgaattaac cctaaagaag tcagctaata caggaacacc ggtagcttat
     2341 aaaatggctg gtcaacaaac tttgatggcg ggggcaattg ataagcccaa tacctacaca
     2401 atcaaaattg atgtggaaac tgagggttct ttgaagatta aaaaagtcga taaagaaaca
     2461 ggaaatgttg taccgggaac cgttttccat ttagactttg gaaaaac
//
LOCUS       DACCQP010000162         2489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCQP010000162 DACCQP010000000
VERSION     DACCQP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.170"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..531
                     /locus_tag="IVO87_002888"
     CDS             <1..531
                     /locus_tag="IVO87_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451008.1"
                     /translation="DENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDI
                     FAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIS
                     TISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILL
                     PENWAELRKLVEYGII"
     gene            518..1939
                     /locus_tag="IVO87_002889"
     CDS             518..1939
                     /locus_tag="IVO87_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7451009.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1952..>2489
                     /locus_tag="IVO87_002890"
     CDS             1952..>2489
                     /locus_tag="IVO87_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7451010.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLF"
BASE COUNT          847 a          315 c          410 g          917 t
ORIGIN      
        1 gatgaaaaca gtaaattgat gaaaaacata caatcaatct ttgggataga agaagttttt
       61 gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta tggcatagat
      121 gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga aactttaatt
      181 aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt gaattccagt
      241 ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt gattagtcct
      301 atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa ctctattatg
      361 ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa agaaacattg
      421 acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa aattttactc
      481 ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta acacactttc
      541 caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt ttattaaggg
      601 aatttcattc ataactattc ctatttttgt aaatattact actcaagcag aatatggttt
      661 gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc taagcctaaa
      721 ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat ttatgtcatc
      781 ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga acatatattt
      841 tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga tgttattaca
      901 aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta actttaacca
      961 ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa tctctgtagc
     1021 tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg gcggaacact
     1081 gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg gtgctcattt
     1141 ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc
     1201 cttagcaaat atagttttag cacaattcga tagaattatg attaattctt atttaggtcc
     1261 tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg
     1321 gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga
     1381 aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat
     1441 tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg
     1501 gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc
     1561 agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta cagtagcaag
     1621 tgctatagtt aatatactat taaacatgat tttcatacct aggttaggaa taattgctgc
     1681 tggatacaca accgtattta cgtatttttt actttttatt tttcattata atatcgctaa
     1741 aaaaattacg aataaacaat tatttgacac aaaaaaaata tgtatcataa taatttttgt
     1801 atcaattttt tctttgatac ttcatatttt aatcaatcaa atgttgattc gttacgtttt
     1861 gacatcatta ttgataatta gtttagttat atatttttta aagattaaga atcgaattat
     1921 agatgactat aattcatagg aggagatata agtgtttaaa ggaaaaacaa tattagtaac
     1981 tggcggaact ggttccattg gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa
     2041 agctatcaga atttatagta gaggtgaaga aaaacaattt tatatgcaac aagagctctc
     2101 aaatcacact aatttgagat ttttaattgg tgatatcaga gactctcaaa ggattgatta
     2161 tgcatgtcgt gatgtggata ttatttttca tgctgccgca atgaaacacg ttccagcaag
     2221 tgaatataat cctatggaag ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga
     2281 tgcagcgatc aaaaataatg taaaacattt tgtaggtatc agtacagata aagtagtgag
     2341 tcctactaac accatgggtg cgacaaagtt attgagtgaa aaattaataa tagctgccaa
     2401 tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta aggtttggaa atgtcatggg
     2461 gtcaagtgga tctgttattc ctcttttta
//
LOCUS       DACCQP010000163         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCQP010000163 DACCQP010000000
VERSION     DACCQP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.171"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1013..1303)
                     /locus_tag="IVO87_002891"
     CDS             complement(1013..1303)
                     /locus_tag="IVO87_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451011.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1511..2203)
                     /locus_tag="IVO87_002892"
     CDS             complement(1511..2203)
                     /locus_tag="IVO87_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7451012.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          733 a          458 c          349 g          943 t
ORIGIN      
        1 tacttgcaaa gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc
       61 ataaagtgtt tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc
      121 ttataatgtt ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact
      181 acgtcatccg ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc
      241 cctattagta acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt
      301 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
      361 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
      421 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
      481 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      541 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      601 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      661 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      721 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      781 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      841 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
      901 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
      961 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
     1021 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
     1081 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
     1141 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
     1201 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
     1261 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
     1321 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
     1381 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     1441 taaaaaaaag aggcagcagt atagattact gttgcttctt tactaattta ggtttacttg
     1501 ttaagaaaga ttatttacgc aaatgttctt caacaatttg ctgagattta tcagctgtat
     1561 cttccacttc atcttttgct ttttctaact tttgaatata ttgtttcaag cttgagtttt
     1621 cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa ctgagtttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACCQP010000164         2481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCQP010000164 DACCQP010000000
VERSION     DACCQP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.172"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            81..1253
                     /locus_tag="IVO87_002893"
     CDS             81..1253
                     /locus_tag="IVO87_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP7451013.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1289..2065
                     /locus_tag="IVO87_002894"
                     /pseudo
     CDS             1289..2065
                     /locus_tag="IVO87_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            2137..>2481
                     /locus_tag="IVO87_002895"
     CDS             2137..>2481
                     /locus_tag="IVO87_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451014.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT         1004 a          374 c          426 g          677 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcgatc aaccaaaata ttcaaaagct tgcgcatcat
     1021 tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc cacaatcgac
     1081 taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga gaaaagatta
     1141 ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt cttatggacc
     1201 tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag tagaaatggt
     1261 aaaaacattg tatagcattt tacacaggag tctggacttg actgaaaaaa tcataaaaat
     1321 atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga agggtctttt
     1381 agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa ttaccgaaga
     1441 aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag ttcgtagaga
     1501 aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt ctgaaatttc
     1561 caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat tatatgcaaa
     1621 taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg aaaatgtatt
     1681 acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata ttgggtcttt
     1741 tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg atagaataaa
     1801 agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga ttttttcaga
     1861 ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag aagttaataa
     1921 aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga aagttgtttt
     1981 agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac aatatagacg
     2041 atctttagaa ttgtcaaaag attaaatata taaaaagcct tcgggctttt tcttttaaca
     2101 attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa aataaatgac
     2161 aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa gagaaaagtg
     2221 tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt acttttgaat
     2281 gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt tggagaatta
     2341 aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta tcgaacaact
     2401 caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt aaaaaacgtt
     2461 aatagtaatc ttatacaaga t
//
LOCUS       DACCQP010000165         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCQP010000165 DACCQP010000000
VERSION     DACCQP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.173"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IVO87_002896"
     CDS             206..2068
                     /locus_tag="IVO87_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7451015.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IVO87_002897"
     CDS             2068..>2397
                     /locus_tag="IVO87_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7451016.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACCQP010000166         2341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCQP010000166 DACCQP010000000
VERSION     DACCQP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.174"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(366..1658)
                     /locus_tag="IVO87_002898"
     CDS             complement(366..1658)
                     /locus_tag="IVO87_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7451017.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="IVO87_002899"
     CDS             complement(1932..2192)
                     /locus_tag="IVO87_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451018.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          356 g          763 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 t
//
LOCUS       DACCQP010000167         2293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCQP010000167 DACCQP010000000
VERSION     DACCQP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.175"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IVO87_002900"
     CDS             complement(51..1598)
                     /locus_tag="IVO87_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7451019.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVO87_002901"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IVO87_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7451020.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IVO87_002902"
     CDS             complement(2043..2237)
                     /locus_tag="IVO87_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451021.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACCQP010000168         2193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCQP010000168 DACCQP010000000
VERSION     DACCQP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.176"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..267)
                     /locus_tag="IVO87_002903"
     CDS             complement(<1..267)
                     /locus_tag="IVO87_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451022.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDD"
     gene            complement(277..1437)
                     /locus_tag="IVO87_002904"
     CDS             complement(277..1437)
                     /locus_tag="IVO87_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7451023.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1437..2123)
                     /locus_tag="IVO87_002905"
     CDS             complement(1437..2123)
                     /locus_tag="IVO87_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7451024.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
BASE COUNT          605 a          477 c          331 g          780 t
ORIGIN      
        1 gtcgtcctca cgcgaatgat cttcttctaa atatttccac cataaatatg gtttaatcga
       61 aggaatatac tctttgtcgt cccgcagatc atcgtacaca tgaacttcaa caccatattc
      121 cttctcaaaa aaaataggaa tctgatcatc gtgtctgctg aaaagcaata cgatatcttt
      181 gattcctagt ttcttcagat tagttaaaca aacttcaagc tcccatttaa accgattgat
      241 tgccggctga caaagaatat acttcattct gatcacctac gcttgtgttg tagttgttgt
      301 ggttgttggt tttgtagttg tagtagtagt tcccaaagcg ctaatatcta atacaatgaa
      361 actatcgtta cgtttaggtt gaccgtttgc atattgttta gctagataaa tgcgttcgtc
      421 ttcaacaaaa tggtattcat ctgaagcttc aatttttagt gtagatccta cacccatgaa
      481 gtaatctgag gctaccccaa taactgcttt tccttctggc acagccgttg actgcaaatc
      541 tgaaactggt actggcaata cttgtacgta ttctccatta gcagttagta cagtcttagc
      601 tgggaatact ttagaccagt aatcagttgg attcacaatt aggaccacat cagaaggatt
      661 cacattacga taaatcggat cattcacacc ttcgatattg aattttgata gtcgcgccat
      721 caaaccgccc atagttacag catctaaagc tgtaataggt tctgcttttt tttcagcata
      781 ttctccgcta gtttgtttgc tcatgtcacg catcattccg actggcatat ctttaccagt
      841 accatcaaca attgcttgtt ctaatgcaat tctcaatgat tctactaaaa cagtacggac
      901 ataacgatct aaccatactg gacctaaatc aagcattgcc ttacatacag gaatataacc
      961 tgatagcttg aactgcttca tgttaattac atcaaagcca ttatctaaaa cttttttaac
     1021 agcttcgcaa agtttacccc accatgctgg attgactcca cgtgacacaa tccattctgt
     1081 tacaccagtt gtgttaacaa aagtaatttt ttgcaatagt ggatgagatt gttctaaatc
     1141 ttcaaataca cgttcaaata cagtagctgg cactaattct tcgacccctg caaaaccttc
     1201 gtttttcact acttcgttat agaattttgt ttcttgtgta gttaatacac gctgaccacg
     1261 gttcattaat actaattgat cttgattttt tgctgttgct tcttctaaaa ttttatcctg
     1321 aatttcctta gataagctta ccatagctgc gctaaaagat tcttcgttac catctttaaa
     1381 agctttcatc aattggtcgc ttgcagctgt tacacctttt aaatttttaa ctgtcattat
     1441 tttacatctc cttgtccaaa tgttttattt aatgctgctg taaatgcagc aattttttct
     1501 gctctttttt ctttaacgtc attcaaaatt tcttcaacgc tttgttcttt tttagcttca
     1561 gtacctgagc tattttctgc atcgataatt tcatcgacca atccataact caaagctgtt
     1621 tctgcatcca taaacgattc tttttcaaga agttcttgca atgcttcatc tgtgccattg
     1681 aatcgtgttt tatatgaagc ctttaccgat ttatcaattg attccagttg gtcagcaatc
     1741 gtacggaagt catcgacatt tccttctccg tatgtggaag cgcggtgaat catcaattgt
     1801 gcattgttgt agatttttat agtatcgcca gccattgcga taattgaagc agcactagcg
     1861 gctaagccgt taatcacaac gttaactttt gctttatttg acttaagtaa gttcccaata
     1921 gcaatccctt gaaatacgtc tccaccgttt gaattaatta ctacttcaat ttcttcttga
     1981 tcacctagac tatccaaaat atttttgatt cccttgtcag tattcccttc aaagaaccaa
     2041 ctagaaccaa taaatccctg aataaaaatt tgcggtactg cgccttcatt ctttactgct
     2101 agaaatgttt tcattgtcgt cattcgcctc acctcctttc gatacttgtt gattgttttt
     2161 agttataaat atttcatctg ccatcgcctt atc
//
LOCUS       DACCQP010000169         2121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCQP010000169 DACCQP010000000
VERSION     DACCQP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.177"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            178..501
                     /locus_tag="IVO87_002906"
     CDS             178..501
                     /locus_tag="IVO87_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451025.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            665..919
                     /locus_tag="IVO87_002907"
     CDS             665..919
                     /locus_tag="IVO87_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451026.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            989..1234
                     /locus_tag="IVO87_002908"
     CDS             989..1234
                     /locus_tag="IVO87_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7451027.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1310..1675
                     /locus_tag="IVO87_002909"
     CDS             1310..1675
                     /locus_tag="IVO87_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451028.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT          595 a          368 c          356 g          802 t
ORIGIN      
        1 aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca caagtttcaa
       61 aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata actattttgt
      121 tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt attctcaatg
      181 ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc ggcagcagct
      241 tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat gatttttcaa
      301 ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa aagatatgcc
      361 cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat catcattctt
      421 tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt agatatcaat
      481 ccattgattt tttctcgata gataagacac attcagcgga acagttcctg cttatcagca
      541 gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt tcctagcatt
      601 tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt gaggagtccg
      661 ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac tggtcgcaag
      721 tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt tgactcttcc
      781 ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg atagtgtttt
      841 tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt atttgatacg
      901 tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata gtcattataa
      961 ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt tactggatac
     1021 tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag tagctgtttc
     1081 tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag aagaaattcc
     1141 aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg attacctaat
     1201 tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg tgctggtttt
     1261 ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta tgcgattatg
     1321 gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac atcgggaatg
     1381 ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt atgtttttca
     1441 ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa tggaaaaacg
     1501 tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat catgcgaagc
     1561 attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac acgatgaagt
     1621 ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta cctaatataa
     1681 ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc ttagatcaag
     1741 ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta aaagcgtact
     1801 ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg acgtggttta
     1861 aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt cttttgatct
     1921 ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat cgatgcttac
     1981 aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag tcactgtgat
     2041 cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc agttcagttg
     2101 ctaagccaga gtcagaacaa c
//
LOCUS       DACCQP010000170         1901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCQP010000170 DACCQP010000000
VERSION     DACCQP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.178"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1..673)
                     /locus_tag="IVO87_002910"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="IVO87_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            751..1790
                     /locus_tag="IVO87_002911"
                     /pseudo
     CDS             751..1790
                     /locus_tag="IVO87_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          553 a          403 c          398 g          547 t
ORIGIN      
        1 cgggaattct aaattcagta atatctgtta accatttttc atttggcttc tttgctttaa
       61 aattccgatt taataaattg gggtgtgcag gagaaatttc cccagcatac gaagaatact
      121 tttttcgtct agaaaattta acaaatagat tttcctcagt catcaatcta cgtacaactt
      181 tttcagaaag ctttttaccc atatttttta aagcttggtg aattctacgg taaccatacg
      241 cacagaagtt atgattgaaa atcgtgataa ttaattgacg ttctttgtaa tacttatctg
      301 gtttctcaag tgcttgtttt tgataaaaat agctgctttt aggcaactgg agctgttgaa
      361 gaagctcttt caaggtgaac tgatttcgta aggcatcaat caatatcgtt ttttcttgat
      421 ttgatagaca aagaagattg acgccttttt ctttttttag cagttcagcc gctccttcga
      481 gtaatgcttt ttcgagctgt aattggtgta cttgtttttg taagtcttct acttccttta
      541 aaagtagctc tttttgtttt tcctgatttt ttaaagtcat acgcaatgga aaatcctttc
      601 caagaaccgt gtttttccat tgatatagag taactctact aattccttca gattcggcta
      661 aatttttcct attatataaa ttgagttaaa ttgtattcgt ttttcctcct ataggcgtat
      721 actaaagaaa acacttgagg agtggggaac atggagacag aacgccaaat ccgtgaattg
      781 gaaaaagcga tgaatacgac agacaaccgc cggttgtacg agcgtttcct ggcagtcaag
      841 ttggttcttg aaggacacac ctggaaagaa gccgggcaca ccatcggccg cgacgagcat
      901 actgtcagcc actatgtgaa aaactactgc cataatggaa tccaaggttt agtctccaaa
      961 aaacaaagcg gacgaccttc acggctgaca gatgaacagg aagcggaact ggtccaaata
     1021 gtcgcctacc acactccgga ggaagtaggt ttcaaatccc gtgccaattg gacattggcg
     1081 attgtgtgtg acctgattct ccgggaatgg agttatggat atacgcaaaa aggcgtggcg
     1141 gacctccttc accggctagg actaagctgg acccgtccga cgtataccct caaaaaagcg
     1201 gattccaaga aacaacaggt atttttggaa gaaaccttcc cgagcctaaa aaaaaaattg
     1261 ctgaatagcg atattgacca cctcctattc gaggacgaaa ccatgatccg ggattaccaa
     1321 gccattggct gcacctggtt tctccgcggc caccagcgaa aaatccccac ttatggacaa
     1381 cacaaagggg taaaattgat aggcaccttg gattatgaag ccggtcggat catttgtaca
     1441 gaagaagggg catacgacgc agcgaccttc ctccgttttc tggagcggac gttgcgggaa
     1501 taccccaccg gaaaaatcgt gatggtattg gataatgccc ttatccacca cgctaagctg
     1561 atccagtctt ttcttgaaga aaacgctagg cgcctggcac tcatcttcct ccctccctac
     1621 agccctcagt tgaacctaat ggaaggcgtt tggaagtggc tcaaggaatc ggtcatcaac
     1681 aatgtttttt tgaccacgtc cagaaaatca aacagtcggt ccgcggtttc ttagccgatg
     1741 taaacgagcg tccccttgag gtgattgacc gactgtgcgt gcggatgtaa agtatttaat
     1801 tcaacctata tagttaattg tagcctatcc aaatcggaat tttaaagcaa agaagccaaa
     1861 tgaaaaatgg ttaacagata ttactgaatt tagaattccc g
//
LOCUS       DACCQP010000171         1878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCQP010000171 DACCQP010000000
VERSION     DACCQP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.179"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1604)
                     /locus_tag="IVO87_002912"
     CDS             complement(<1..1604)
                     /locus_tag="IVO87_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451029.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLAEPYNMCREYPNENIANPTVKFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRILQSPKETASACQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEIT"
     gene            complement(1628..>1878)
                     /locus_tag="IVO87_002913"
     CDS             complement(1628..>1878)
                     /locus_tag="IVO87_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451030.1"
                     /translation="RVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETS
                     KPNVLVRWNLVAHRLGYEDVRLKEDTAYDSTVLDQKRF"
BASE COUNT          512 a          382 c          323 g          661 t
ORIGIN      
        1 gtgatttcag caaccttgat atctgtagct aggttattct caacatagct aggttctaga
       61 tcccatacat aatcttttgg attgtttgag tctcgcatgc cgattcctcg gtatttatat
      121 tgctcaatat tcggggttcg tgtgtcgcct ttttcaatct tgagccagtc aatttggcat
      181 gcgctggccg tttctttagg cgactgcaaa atgcgaaggt ctttaggcaa acccggttca
      241 agtttcgttg gtgtgaatgt tagagaccat acgtctgtca atccttcaac tggttttagg
      301 tctccaaaat taacgttcca ataattatat gctacaaagg tttgacttgc gggttttgtt
      361 cctttaagcg tgatagtata cgtttgacct attataagct cttcttccgt gttaccttta
      421 tatatttcgt acgcgctaga tttaattggg aacttaactg tagggttggc aatattctcg
      481 ttaggatatt cgcgacacat gttgtaaggc tctgctaata agtttggctg atatggggtg
      541 gctgttgagc cttcttcgat tttgatgtcg tacaacttaa atccgccatt tattttatcc
      601 ctgtcaacaa agctaatact catataaaat cgatctaaat ttgtgatttg ataattaacg
      661 ttagctgtac ctttgattgt tatttctttc cctacatcat ctgttgtaat atttgtacta
      721 tttgcttcca ataatatctt ttctcctggt gatgtacgat acaccaaacg taatttatca
      781 atagctcctg tagttccttc atcaaatcga actttcgcac tcagagtata ggttttccca
      841 ctagcaagct gaggtgtatt aatacgcgta aacataataa tgcttcctgt accatcagaa
      901 gtaaaatgta acttttcgtt atccaaagtt agtgacccgt ggtaaccaac gttaaaatcg
      961 ctcgatttta gtttggacat taaattcgga ttcccactat aatcatagcc cccgaagtcg
     1021 atgctgttgg agtacatcac atgtaagtta cctaatttag aaatttcttc tttcagagca
     1081 tctaacttgt cttgtagtgt tttagcttga ccagttaaat cagtaatctg tttatttaag
     1141 ctatccattc tacctttaat ttcagccaga aaagcatcaa aagtttcgtt atactttcga
     1201 atcaattctt ctaattgcga aacatattca tcggcttggc cttgcgaaat gtcagacact
     1261 cctagtgaga aaaaaatgat atcttgcgtc gttaaaattt gatcgtcttt tctatattct
     1321 acatagcagt gtttataata ccctgcttca ctcataaatg tgccatcaag agaaaatgtg
     1381 acttcttcac tagttacact agtagcgaca ctatctacgt aacggttaga tggcgtagtt
     1441 ccttttaaag taaatgttcc gccgctcgta tccatctgca agccatttaa aaacggttta
     1501 acagtcaccg tgatcccttt atcaccttga cgagccataa tagctttggt gtagtttaat
     1561 tctttgctga aatctaaagc caaattatat aaactgctag ccatttatat acctccttgt
     1621 cttcgtttta aaaacgtttt tggtcaagca ctgtgctatc atatgctgta tcctctttta
     1681 atctaacatc ttcataccct agtcgatgtg ccactaaatt ccatctaacc aatacgtttg
     1741 gcttactagt ttcaatgatg aaatggtcaa catcttcatg agtaacagca cacaaaacta
     1801 gttctgtagg tgtcacatgt gtcatatacc gacttagatt tactgtctca gcaaacatgg
     1861 ggtcaatatc aacacgaa
//
LOCUS       DACCQP010000172         1764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCQP010000172 DACCQP010000000
VERSION     DACCQP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.180"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(430..1002)
                     /locus_tag="IVO87_002914"
     CDS             complement(430..1002)
                     /locus_tag="IVO87_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7451031.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1018..1623)
                     /locus_tag="IVO87_002915"
     CDS             complement(1018..1623)
                     /locus_tag="IVO87_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7451032.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          516 a          361 c          260 g          627 t
ORIGIN      
        1 taatatcaca ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt
       61 tatttggttc ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa
      121 atcaatagag tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac
      181 gtgacttacg ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata
      241 tatgatggta tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta
      301 cagttaccat acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac
      361 aacatgctaa tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag
      421 acttcttttt taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa
      481 accggttata gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg
      541 cctcttcttc ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt
      601 tcttgcaaac atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc
      661 tacactcaac aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag
      721 attcaaaacc ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc
      781 tctcgtgtta cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag
      841 accagccaac agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga
      901 aaaaatattc ttcttctcta cgccgttctc aagcaactgt tgaatttgtt cttttgtact
      961 ttgagaagcg gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta
     1021 caaatcatct atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt
     1081 catatataac tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat
     1141 ttccaaatcg tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa
     1201 gttccaatct acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt
     1261 acttcggcca tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat
     1321 aatctcttca aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata
     1381 cataactggt gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc
     1441 aaaatcgtaa atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc
     1501 aacttcgatt tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag
     1561 tttttcttca accttagcca actcaacttg attggttaat cccaatttat tctctaaaat
     1621 catgcgaaat ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata
     1681 tcgcgatttt gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa
     1741 gtgtctctat tttgcacagt ctag
//
LOCUS       DACCQP010000173         1717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCQP010000173 DACCQP010000000
VERSION     DACCQP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.181"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1649
                     /locus_tag="IVO87_002916"
     CDS             <1..1649
                     /locus_tag="IVO87_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7451033.1"
                     /translation="IGEAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANF
                     IYSILFTQVVGKSTNRMRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQ
                     ALLQVITNAVLLVGILIMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDE
                     VGKLNGYMDEKISGQRVIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQG
                     MSLVNTAIVIFFGGWLALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLA
                     ITGARRLNEIFDEKDEVKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKG
                     EMVALVGPTGSGKTTIMNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESV
                     LFSGTIKDNITFGKPDASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQK
                     QLISIARTIITNPSLLILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILN
                     ADRIVVLRDGEVIEEGSHHELLAENGFYAELYHNQFVFES"
BASE COUNT          558 a          281 c          378 g          500 t
ORIGIN      
        1 acatcgggga agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag
       61 acgataaaag caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta
      121 gtgcagccaa ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc
      181 gaatgcgtat cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc
      241 atcaagacgg tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt
      301 tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca
      361 tgatgttccg ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg
      421 ttttgatagc tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg
      481 aagtggggaa actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca
      541 caaatggctt acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg
      601 aagcaacctt caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt
      661 cactggtcaa tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt
      721 tggaacgttc ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt
      781 accaaccact gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg
      841 caagacgatt gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag
      901 catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag
      961 aaaaactagt cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg
     1021 ttggtccaac aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg
     1081 tcaatgatgg ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat
     1141 tacgtagaca tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag
     1201 acaatatcac attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac
     1261 aagcgaatat tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg
     1321 aagaaaataa cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca
     1381 tcacaaatcc atctttattg attttggatg aagcaacaag taatgtagac acggtaacag
     1441 aagcaaggat ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg
     1501 ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag
     1561 tcattgaaga aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt
     1621 atcataatca atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt
     1681 tttatatatt ttatgttgtt tgtttttata aggggca
//
LOCUS       DACCQP010000174         1717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCQP010000174 DACCQP010000000
VERSION     DACCQP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.182"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(201..599)
                     /locus_tag="IVO87_002917"
     CDS             complement(201..599)
                     /locus_tag="IVO87_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7451034.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(596..832)
                     /locus_tag="IVO87_002918"
     CDS             complement(596..832)
                     /locus_tag="IVO87_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7451035.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(992..1309)
                     /locus_tag="IVO87_002919"
     CDS             complement(992..1309)
                     /locus_tag="IVO87_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451036.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            complement(1447..>1717)
                     /locus_tag="IVO87_002920"
     CDS             complement(1447..>1717)
                     /locus_tag="IVO87_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7451037.1"
                     /translation="LNPECDFKLWPTVAKKKLTKTNLRDLLSKGKTSKVVKGFTGKKG
                     KFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENEVE"
BASE COUNT          521 a          316 c          235 g          645 t
ORIGIN      
        1 taaaaaatca gcccactagt tctaaaccac tatttcctac tattcactct tttgtattcc
       61 ttttaaatca attatttcaa aattaatcca tattttccat atttttatga aaaaactatt
      121 ttgtatgatt aaacataaaa aaatcagcat attccttaat gacatgctga tctaattgag
      181 aaataaagtt tttaattcgt tcattttatt gcggtttgtc gtaaataact ggttatcttg
      241 gactttagtg aatcgaaatc caatgaacta gtggtaatca ttatgataaa atcaacagct
      301 tcttgacgag aaaaagcaac tggacaatta ttcaattgaa gaaacaacaa catggcaaca
      361 agagcagttc gtttattgcc attatgaaaa ggatgttttt tagctaaatt aattgctaaa
      421 atcgccgctt tttcatagat ttcaggatag agttcttgat tgaatactgc ttgtgaaggc
      481 tgtttgacgg ccatatctaa tgcatttgca tcttttacac cgatcatttc gcctggcgag
      541 tgacgtaaaa taactttcgc gttgattttg ataatttctt tagtcgaaag atacctcata
      601 aatcaaccaa atcttttaac gcgtcttcat attgattcat gatcgaatca actaatgaat
      661 caatatcagg ttcttcttgt gctttgatca ttcgtacaga accatcagat tgaaaaacat
      721 aactgattgc gtcacctgtc tgcacaccca tgaattcttt tacttctggt gggatagttg
      781 ttacatcgct attgccagat tttctaattt tatacttttt catttttttc atatttcgcc
      841 accctctata ccattctcat tctatagtaa gtacattata tccaagtata tacttttaat
      901 caatgtatat acttttattt tgctttttct tacgaaaaaa tgacaaagaa aaaagtttgt
      961 gacatgactt ttgtcacaaa ctctcgtatt cttaaccaaa atattgatga aatacttgtg
     1021 taataactgg tagaaaataa ataatgatca tagccgctac caccaccaac aattgttttc
     1081 gctgtttctt tcgctttagc atgcttgtcg ttttatgaaa aaaataagta gcgcttttag
     1141 ctcctgcagt caaagtcgat aaaatcaacg ttggtgaata tgctgtttga ttgtgcgtca
     1201 atgacaacaa cttcgtcccg taatcaggat actttagaaa tgcttggtac acagccacaa
     1261 tcgtgattgc tcccataaga taatttaaaa atgaatgcac ttttttcaaa taaaaaaact
     1321 ccttgctatc tatataaaat cagcctaccc caacgttttt gcttgattca ctgatatttt
     1381 ttagttttat tggtatttat attctcactt ttcggaataa atattccgct atttcattgt
     1441 tttcatttac tctacttcgt tttcattatt ttttcttgat gtttctttgt ttccatcaac
     1501 tgcttttgtt ccactgtttt cgatccatgg aaaagaaaaa ccgattgtca tatcttcttt
     1561 taatactaaa actgcatcga atttcccttt tttaccagta aaacctttga caactttact
     1621 tgtctttcct tttgaaagca aatctctgag attcgtttta gtcagcttct ttttagctac
     1681 tgttggccac aatttgaagt cgcattccgg attcaag
//
LOCUS       DACCQP010000175         1608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCQP010000175 DACCQP010000000
VERSION     DACCQP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.183"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVO87_002921"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IVO87_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7451038.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IVO87_002922"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IVO87_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          307 c          305 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACCQP010000176         1552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCQP010000176 DACCQP010000000
VERSION     DACCQP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.184"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="IVO87_002923"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="IVO87_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP7451039.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            146..880
                     /locus_tag="IVO87_002924"
     CDS             146..880
                     /locus_tag="IVO87_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP7451040.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          581 a          243 c          211 g          517 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aaggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      181 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      241 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      301 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      361 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      421 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      481 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      541 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      601 atccatatta agtaaaatcc ctagagaata ctttcatcca aaacctagag ttaatagctc
      661 gttaattgta ttaaaaagac acccttcaaa aatatcactc aaagatagaa aacaatatga
      721 aaattttgtt atgaaatggg ttaacaaaga atacataaaa ctattttcca aaaaccaatt
      781 ttatcaagcc ttaaaatatg caagaattga cgatttaaac aatattagct ttgaacaatt
      841 cttgtctctt ttcaatagct ataaattatt taatagataa gttaaggggt gcataaactg
      901 catcccttaa cttatcttat tttgcttaaa caatcttggc ccgtaaactg cataactcat
      961 atgtaaatcg ctccttttta ggtggcacaa atgtgaggca ttttcactat ttccggcaat
     1021 tacttccatg taaagtataa cccactatac tttgcaagca aagtgtgggc tcgccgaggg
     1081 gtcttgcaga cggctctttc ggctcttttg agccgagtat cagcgcaccc ttatggtgca
     1141 attagctgat acctgtttga cgatttgctg ccctaccttt tcggtaggta tgcaaacctc
     1201 aaaccaaaat aaaaaccccc ttcgacttcg tcagggggtt tttatttata taaatctccg
     1261 taaataatcg cgatttaata acatcttcaa ttttattttt ttgcgtattt tagaaatgtt
     1321 ttcactaaaa aatatattat acaaaaaata cagtaatcga ttataattct ttgccagtta
     1381 aatatcatat cgttattaaa atatcggaaa aaaatatcaa caacaataaa tgcaaaagaa
     1441 gaccataaaa attgtttatt actcatacat actctccatt tctatcattt ttaaatttac
     1501 cagtcatctc tcaaaaatct cctagctatt tttctttttt aaccacatac cg
//
LOCUS       DACCQP010000177         1492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCQP010000177 DACCQP010000000
VERSION     DACCQP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.185"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            95..505
                     /locus_tag="IVO87_002925"
     CDS             95..505
                     /locus_tag="IVO87_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7451041.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            519..875
                     /locus_tag="IVO87_002926"
     CDS             519..875
                     /locus_tag="IVO87_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7451042.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          546 a          219 c          274 g          453 t
ORIGIN      
        1 tattttgatt tttttttata ttttattagc taataattgg tattttaaaa gaaattactg
       61 tagaattgga ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag
      121 ctgtacttcc tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga
      181 gaagaatatt tttgccgaac cgttaacaga aagtgagatc aaaaagacct tggcagcgac
      241 tgaaggagga gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat
      301 agattttaac gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt
      361 attgagacga cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga
      421 gatccggtca tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca
      481 aaacaataac atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat
      541 cagcgcactg tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt
      601 atcaatttgt tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca
      661 gttttgaaga agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca
      721 ttgcgattga cctttcctgt gtagaaaaaa aacaactgat ttttttatgc acacaacata
      781 ccaacctcct cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg
      841 agcagttgat ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac
      901 tgagcattcc atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt
      961 tttttgactt tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat
     1021 ctaggtgggg atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca
     1081 tttctgttgt tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt
     1141 tgttgacatg ttagaaatga catgataaga taactgagtc gttaaaaaag gaccgcttgc
     1201 gaagcttgat tctatcgaaa aaacttcaaa aaagttcttg acaaataaca aacgacaagt
     1261 tatgataaat gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag
     1321 agttgacaat tacttgtctc tctgatatga tataaaagtt gctgcggcag ccaaaaacga
     1381 atcgggaaac actcctgatg aagtagacct ttgaaaaatg aacaaagtaa gacaaaccaa
     1441 atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag ca
//
LOCUS       DACCQP010000178         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCQP010000178 DACCQP010000000
VERSION     DACCQP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.186"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IVO87_002927"
     CDS             complement(150..1337)
                     /locus_tag="IVO87_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7451043.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCQP010000179         1389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCQP010000179 DACCQP010000000
VERSION     DACCQP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.187"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IVO87_002928"
     CDS             123..1292
                     /locus_tag="IVO87_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7451044.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          501 a          226 c          242 g          420 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacacataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgag
//
LOCUS       DACCQP010000180         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCQP010000180 DACCQP010000000
VERSION     DACCQP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.188"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            20..>1371
                     /locus_tag="IVO87_002929"
     CDS             20..>1371
                     /locus_tag="IVO87_002929"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7451045.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     "
BASE COUNT          458 a          234 c          293 g          386 t
ORIGIN      
        1 gagataggag tgtatctttt tgggtgcaga attaaatcaa aaattattta gtgcggcaga
       61 taatctccgt agtaaaatgg atgcgtcaga atataaaaat tatttattgg gactgatttt
      121 ctataaatat ttatccgatc gattgttgga acaagtggtt cttttagcag atgaatcctt
      181 agaggaatat gacacagtaa gcaaacaaac aatgttgtat cgtgaattac tttcagatga
      241 agaatcaaaa gaagatctga ttgccacaat cgttgatatt ttgggatatg caattgcacc
      301 agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaacga
      361 tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga
      421 tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat
      481 taccgaagtc atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat
      541 cggggatgcg tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg
      601 agaattttac acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca
      661 aaaggaacga cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt
      721 aaacgtacgt aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa
      781 tacaacgaca tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga
      841 aatgaatctc cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac
      901 atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt
      961 cttagatgat tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt
     1021 tgcgtttctt cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt
     1081 accacacgga gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga
     1141 agatggcagt atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat
     1201 tccaacgacg gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga
     1261 tgctagtcgt gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca
     1321 aaaaatcctc gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc a
//
LOCUS       DACCQP010000181         1370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCQP010000181 DACCQP010000000
VERSION     DACCQP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.189"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..843
                     /locus_tag="IVO87_002930"
     CDS             <1..843
                     /locus_tag="IVO87_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7451046.1"
                     /translation="TKFVCAIGDEEMTIKERVSFPTTTPEETMPLVIDFFKQYQADLA
                     GIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAY
                     GEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGS
                     CPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVI
                     IFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREA
                     VLHS"
     gene            complement(1077..1370)
                     /locus_tag="IVO87_002931"
                     /pseudo
     CDS             complement(1077..1370)
                     /locus_tag="IVO87_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          402 a          265 c          304 g          399 t
ORIGIN      
        1 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
       61 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
      121 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
      181 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
      241 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
      301 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
      361 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
      421 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
      481 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaacgc
      541 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
      601 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
      661 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
      721 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
      781 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
      841 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
      901 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
      961 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggctacttg
     1021 tattgtgcaa tagtttttta caaaaaaaag gggtgcggat atttttttgg acctttttaa
     1081 caaatatatc tttgcttctt ccgatattga agagggctta gccctcctaa agataactta
     1141 attcgttgat tattatacca atgaatataa ttatccaatt tgtttataaa ctgattgatc
     1201 gttgtattct tccagtcacg ttgataaaac atctcgtttt ttaatcgtcc aaagaaacct
     1261 tcgcacgctg aattatctgg tgaataaccc tttttagaca tagagtgaag caagttgtct
     1321 ttgttccaat agtccaacta acaatagcac catcgtaaca atcgacaagt
//
LOCUS       DACCQP010000182         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCQP010000182 DACCQP010000000
VERSION     DACCQP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.190"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            21..1322
                     /locus_tag="IVO87_002932"
     CDS             21..1322
                     /locus_tag="IVO87_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7451047.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          439 a          270 c          252 g          395 t
ORIGIN      
        1 caatcaaagg agcgttctgt atgcctgatc ttcatcatat caaactacta cttggaatca
       61 aagataaaaa cattttcatc acaaatgtgg aatcgaagag cattaagaat atcaagagtc
      121 ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg tcctttgtgt aaacagatga
      181 atcatgaagg catgatcgtt aagaacggaa agaaaaaatc gctcattcaa cttaataaat
      241 gtgcgaacca actgacttat cttgctttag cgaaacaacg ctaccactgt cgtggctgcc
      301 acacctattt tacggcgaat acctacattg tcgatagaaa ctgctttatc gctaaacaag
      361 tgcgctataa aattttagaa gagctaactg aaaaacaagc aatgacgaca attgctaagc
      421 attgcggcgt ttcttggtcg accgtttctc ggacactcgc ttctctgatc cccatgacaa
      481 aagtaaaaag aaattggtta ccgcgttgtt tattaatgga tgaatttcgt tcactgaaaa
      541 atcaagtcgg tccctactcc ttttcttgta tggatggcga tactggaaaa ttactggata
      601 ttcttccttc acggaagaaa aaggctctcg tttcttattt tatgcaattt gaacgacgcg
      661 cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt ttcttatgcc tctctgataa
      721 aggaatgttt tccaaaggca aaactcgttg tggatcgttt tcacattgtc aaacatctca
      781 ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag ctttgatcga aacgatccaa
      841 cacaagccaa atattatcga caattaaagg cgttgtctcg gcttctgatc aaacgacagg
      901 acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt tggttgggcg tatctcaccg
      961 aaagtgaagt ggtggatcgt ctgctttcga tctctgacga attacgaatc gcctattttt
     1021 actatcaaga aattcttcaa gcgtttcacg acaaagaggc ggacactttc tttaaacttg
     1081 taagaacgat gcccaattcc gttccaaaag aactgcattc tataaaaaaa gcttttataa
     1141 actatgaatc cggcattcgt ttagccctcg aattgcctta ttcaaatgcc aaaatagaaa
     1201 atctgcatac ccacatcaaa gcattgaaac gagtcgctta tggctttcga agctttcgga
     1261 aaatgaagac cagaattttc ttactaaata atctgattac ctatgaatca aaaaacatct
     1321 aaagtagaaa aggaattcta ccttagatgt tcttat
//
LOCUS       DACCQP010000183         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCQP010000183 DACCQP010000000
VERSION     DACCQP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.191"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1121)
                     /locus_tag="IVO87_002933"
     CDS             complement(<1..1121)
                     /locus_tag="IVO87_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451048.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLIN"
     gene            complement(1131..>1328)
                     /locus_tag="IVO87_002934"
     CDS             complement(1131..>1328)
                     /locus_tag="IVO87_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7451049.1"
                     /translation="EGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQYPTLQV
                     DINYFNCTGLSKIQIYRNGLR"
BASE COUNT          386 a          281 c          185 g          476 t
ORIGIN      
        1 tcattaatca aattgctgac attattctct gtgttttcta agaagtttgt tttgacgttt
       61 cctacaacta atttatcgta tgaattggtt agaacattaa acgtatattc cacaattctc
      121 gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc catcgaaact
      181 ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac taattcaatc
      241 gaaccactca cttgtggttt ttgtttatct atgtttgttt tcaaccagtc tttagcagct
      301 tcccttaatg tggctacatc agtcgctttg tctttaaaat caacaaaaga aacatatcca
      361 gcaggataat catccacgta atccgtgaaa ataacttctt ctggtagagt gatctcgtct
      421 tctccttctg aagagctgct aatgaatgga taaactccaa ctaaaacact ttgagcatca
      481 atctctaagt caagaccagt taagttttta gtataaatcg ctttgatttt atgatccgtg
      541 cctagccttt tttcatgacg taatgtgtta ttatctttta gaaattcccc atgaaatcga
      601 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      661 acattagcaa gcgtatctac taatgacgag aaagaaaact gtgaaggaat aactggtttc
      721 gctaaagttt ttgcgttttg ccatgcctga ctagcagtga tcttttctgt tccactgtca
      781 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      841 gtattgccta ttacagaagt ctttgcataa taaatccgta gacgctgttt tgctcgattt
      901 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgctt
      961 attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac agtagctgtt
     1021 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
     1081 gcgtcgtttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
     1141 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaataatt aatatccact
     1201 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
     1261 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacgtcagt tcctactact
     1321 aagccttc
//
LOCUS       DACCQP010000184         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCQP010000184 DACCQP010000000
VERSION     DACCQP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.192"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            102..>1316
                     /locus_tag="IVO87_002935"
     CDS             102..>1316
                     /locus_tag="IVO87_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7451050.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKR"
BASE COUNT          414 a          260 c          253 g          389 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgc
//
LOCUS       DACCQP010000185         1264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCQP010000185 DACCQP010000000
VERSION     DACCQP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.193"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            153..1201
                     /locus_tag="IVO87_002936"
                     /pseudo
     CDS             153..1201
                     /locus_tag="IVO87_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          507 a          143 c          245 g          369 t
ORIGIN      
        1 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
       61 accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat aaaaaattat
      121 atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct ataaaatgct
      181 attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga ggagcatgcc
      241 tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca
      301 ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca attaatgcaa
      361 atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag aaagaatatg
      421 tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat tatatagctg
      481 ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta aagtatgcta
      541 ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa aagatgggcg
      601 atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt atatttgaaa
      661 aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata ctaaaatagg
      721 agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag ctgaaagtaa
      781 tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag aacgtcctaa
      841 aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat ttaacaaaga
      901 atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg attttacaag
      961 taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt ggtgtacagc
     1021 ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa gcaagggtgt
     1081 aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca aggaagcaat
     1141 tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct taatgccaga
     1201 tggaatggca acactttttt tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat
     1261 ggct
//
LOCUS       DACCQP010000186         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCQP010000186 DACCQP010000000
VERSION     DACCQP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.194"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /locus_tag="IVO87_002937"
     rRNA            <1..>1231
                     /locus_tag="IVO87_002937"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACCQP010000187         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCQP010000187 DACCQP010000000
VERSION     DACCQP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.195"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..345
                     /locus_tag="IVO87_002938"
     CDS             <1..345
                     /locus_tag="IVO87_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451051.1"
                     /translation="KVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGPKVH
                     EELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKKCS
                     YAYVKAIKAVVR"
     gene            342..653
                     /locus_tag="IVO87_002939"
     CDS             342..653
                     /locus_tag="IVO87_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451052.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            643..996
                     /locus_tag="IVO87_002940"
     CDS             643..996
                     /locus_tag="IVO87_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7451053.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            986..>1215
                     /locus_tag="IVO87_002941"
     CDS             986..>1215
                     /locus_tag="IVO87_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451054.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSE"
BASE COUNT          442 a          177 c          235 g          361 t
ORIGIN      
        1 aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata
       61 caaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt
      121 cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa
      181 gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa
      241 gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa
      301 tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga
      361 ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg
      421 agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa
      481 cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg
      541 actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca
      601 ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga
      661 ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat
      721 gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat
      781 ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag
      841 aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc
      901 gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat
      961 ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt
     1021 aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt
     1081 tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac
     1141 aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac
     1201 tttctattca gaaaa
//
LOCUS       DACCQP010000188         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCQP010000188 DACCQP010000000
VERSION     DACCQP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.196"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IVO87_002942"
     CDS             <1..1076
                     /locus_tag="IVO87_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7451055.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACCQP010000189         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCQP010000189 DACCQP010000000
VERSION     DACCQP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.197"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            82..>1176
                     /locus_tag="IVO87_002943"
     CDS             82..>1176
                     /locus_tag="IVO87_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7451056.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVK"
BASE COUNT          404 a          187 c          238 g          347 t
ORIGIN      
        1 aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg tggttacaag
       61 gtgcgctgga ggtgttcaaa catgagcaat gatacacaac cagaaatccg tttcccagga
      121 tttacagatg attgggaaca gaggaagttg ggggacttcg ttgtagatta tgttgaaaag
      181 acaagtgtac aaaatcaatt tcctatgcta acttcatctc aacaaaaagg gatagttttg
      241 caagaagact attttgcaaa tcggcaagta actacagaaa ataatattgg ttactttgta
      301 ctacctagag gatatttcac gttccgtagt cgaagtgata atgatgtgtt tgtgtttaac
      361 cgtaatgaca ttattgatag agggattata agctattttt atcctgtatt tactttaaaa
      421 tctgctgatt ccgacttctt cttaagacgt attaacaatg gaatccaacg acaattatct
      481 attcaagcag agggaacagg tcaacatgtt ctgtctctaa aaaagtttaa aaatatagta
      541 gcaatgtttc ctagtgagga agaacaacaa aaaatcggca ccttcttcaa acaacttgac
      601 gacacgatcg ctcttcatca gcgtaagtta gatttgctga aggaaacgaa aaaaggattc
      661 ttacaaaaaa tgttccctga aaacggagca aaagtgccag aaatccggtt tcctggtttt
      721 acggaagatt gggaagagcg taagttggga gaattaggta aaactcaatc gggaattgga
      781 tttcctgatg ccgagcaagg tggttctgaa ggtactccat tttttaaagt ttccgacatg
      841 aataacattg gtaatgagta tgaaatgaga aatgcaaatc attatgtatc taatgagcaa
      901 attgagcgta aaaaatggaa gccaataaaa gatgtgccag cgattatatt tgctaaagtt
      961 ggcgcagcaa taatgcttaa tagaaaacgt ttggtcacaa gccctttctt aatagataac
     1021 aataccatgg cttatttatt taataatact tgggatatct attttggtaa aatattgttt
     1081 gaaactataa atttgccaag atactcccaa gtaggggcat tgccttctta taacagttct
     1141 gatattgaga atatcagtgt taaggttcct gtaaag
//
LOCUS       DACCQP010000190         1133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCQP010000190 DACCQP010000000
VERSION     DACCQP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.198"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(176..>1133)
                     /locus_tag="IVO87_002944"
     CDS             complement(176..>1133)
                     /locus_tag="IVO87_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7451057.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT          332 a          242 c          174 g          385 t
ORIGIN      
        1 cgaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
       61 taagatgttg tttttacttt gctattctta ttacttgatg caggacagct ttttcacaac
      121 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
      181 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
      241 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
      301 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
      361 cgcaatttgc gccttcttct gttgctttga tcaaataacg caaatgctgt ccgataaatt
      421 cgatacggta atcatcttgc acgattcctt tctcatccat ataccgttct tcatgttctc
      481 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
      541 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
      601 tcccaggtaa atcaaagact tcataatatc gttctggatg gaaaggtgtc tcttcattcc
      661 attgataacg aggagcgcga acgcgtttag gataatattg attgattcca aggaaatcta
      721 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
      781 tttgacacaa ctccaggaac tcttctgtgt agtacccttt taccatcgga tcaaaaaata
      841 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattagatc
      901 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
      961 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
     1021 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
     1081 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tca
//
LOCUS       DACCQP010000191         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCQP010000191 DACCQP010000000
VERSION     DACCQP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.199"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IVO87_002945"
     CDS             complement(36..992)
                     /locus_tag="IVO87_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451058.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACCQP010000192         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCQP010000192 DACCQP010000000
VERSION     DACCQP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.200"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IVO87_002946"
     CDS             6..980
                     /locus_tag="IVO87_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7451059.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACCQP010000193         1022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCQP010000193 DACCQP010000000
VERSION     DACCQP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.202"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            265..798
                     /locus_tag="IVO87_002947"
     CDS             265..798
                     /locus_tag="IVO87_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7451060.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          334 a          189 c          185 g          314 t
ORIGIN      
        1 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
       61 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
      121 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
      181 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
      241 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
      301 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
      361 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
      421 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
      481 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
      541 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
      601 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
      661 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
      721 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
      781 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
      841 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
      901 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
      961 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     1021 gg
//
LOCUS       DACCQP010000194         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCQP010000194 DACCQP010000000
VERSION     DACCQP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.203"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..563)
                     /locus_tag="IVO87_002948"
     CDS             complement(<1..563)
                     /locus_tag="IVO87_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7451061.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
     gene            complement(574..780)
                     /locus_tag="IVO87_002949"
     CDS             complement(574..780)
                     /locus_tag="IVO87_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7451062.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(913..>1018)
                     /locus_tag="IVO87_002950"
     CDS             complement(913..>1018)
                     /locus_tag="IVO87_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7451063.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          286 a          219 c          162 g          351 t
ORIGIN      
        1 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
       61 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      121 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      181 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      241 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      301 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      361 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      421 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      481 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      541 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      601 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      661 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      721 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      781 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      841 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      901 ctttcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac tcaaaaatac
      961 ttccaaaggt gttcgataat ccagtgattt tctaggaata ttatttcgtt tagatgcg
//
LOCUS       DACCQP010000195         1005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCQP010000195 DACCQP010000000
VERSION     DACCQP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.204"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            271..972
                     /locus_tag="IVO87_002951"
     CDS             271..972
                     /locus_tag="IVO87_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7451064.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          365 a          165 c          156 g          319 t
ORIGIN      
        1 ttattcattg aaaaaatttt tttgaataaa ttaaaaaaat aaattatatt atcgttgtta
       61 gcgtttacta tatgtattat tgttgttttt tatacacaaa tcattcgctt caaaacaaat
      121 tattcaatac cattgtatat tttttacata ataacaaatt atatttgtaa agtattaaaa
      181 cttgtttcta atgttggtat gttaaaactt tacaaattct ttaaaaaaag tgacaataaa
      241 tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga ttttcacttt
      301 tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt ccaacagatc
      361 aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga cgatggtgat
      421 cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg ttatgatgaa
      481 agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc tatgtcagga
      541 atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat tgaaacattc
      601 taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca gttgatctat
      661 atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca aatcggattg
      721 acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga agaattgggc
      781 gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa agaattagca
      841 gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact acgtgattcc
      901 gggctgttag attatgacaa aaagcgtttt actttctgta agacagctac tactccagct
      961 atgagagaat aacgcgaaca aaaataagca gtgaccctga taaat
//
LOCUS       DACCQP010000196         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCQP010000196 DACCQP010000000
VERSION     DACCQP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.205"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            369..1000
                     /locus_tag="IVO87_002952"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IVO87_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACCQP010000197          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCQP010000197 DACCQP010000000
VERSION     DACCQP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.206"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IVO87_002953"
     rRNA            2..>943
                     /locus_tag="IVO87_002953"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACCQP010000198          916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCQP010000198 DACCQP010000000
VERSION     DACCQP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.207"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            1..916
                     /locus_tag="IVO87_002954"
                     /pseudo
     CDS             1..916
                     /locus_tag="IVO87_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          338 a          157 c          174 g          247 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacac
//
LOCUS       DACCQP010000199          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCQP010000199 DACCQP010000000
VERSION     DACCQP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.208"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IVO87_002955"
     CDS             <1..888
                     /locus_tag="IVO87_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7451065.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACCQP010000200          874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCQP010000200 DACCQP010000000
VERSION     DACCQP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.209"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>874
                     /locus_tag="IVO87_002956"
     CDS             <1..>874
                     /locus_tag="IVO87_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7451066.1"
                     /translation="KLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAII
                     SLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTID
                     DLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINEC
                     IEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYI
                     LDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDK
                     IFDVKFDLNKENSNG"
BASE COUNT          388 a           71 c          118 g          297 t
ORIGIN      
        1 aagcttaatg atggtaaaaa aacaaatcta caatttgttt ctacagctga tatgcaaaaa
       61 gatgcgaaag atttaagcct taaattttta ccatattctt tattaatatc aattttattt
      121 tctgctatta tttctttaat ttatgcaaaa tcaataaaaa ataatataca agaaataaaa
      181 attgttactg ataaaatgat gaaacttgat aaaaaaatga gtttaaaagt tagctctaat
      241 gatgaagttg gagaattaaa acaacaaatt aatgatttgt attctacttt attaagaaca
      301 attgacgatt tggaatttaa aaataaagaa attttaaaat tagaaaagtt aaaatatgac
      361 ttttttaaag gtgcatccca tgaacttaaa acccctcttg ctagtcttaa aataatactg
      421 gaaaacatga aatacaatat tggaaaatat aaagaaagag atatttatat taatgaatgt
      481 attgaaatcg ttgatagttt aacaaaaaat atttctcaaa tattatcagt tcattctata
      541 gaaaatttga ataatgatga agaatatttg aaaataaatg atacattaga agacatatta
      601 aaaaagtatg aaatattagc aaatcaaaag aaaataactg tcaataatta tatattagac
      661 gagaatgttt atataggaaa aacagcttta aagattattc tatctaattt gattagtaat
      721 gcggtaaaat atactgatgt aaatggggta attaatattg ggatagttaa tgattggtta
      781 tatatagaga attcatacgg taacaataaa atttcgaata tggataaaat atttgatgtt
      841 aaatttgatt taaataagga aaatagtaat ggat
//
LOCUS       DACCQP010000201          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCQP010000201 DACCQP010000000
VERSION     DACCQP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.210"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            143..258
                     /gene="rrf"
                     /locus_tag="IVO87_002957"
     rRNA            143..258
                     /gene="rrf"
                     /locus_tag="IVO87_002957"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            272..344
                     /locus_tag="IVO87_002958"
     tRNA            272..344
                     /locus_tag="IVO87_002958"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Val,seq:tac)
     gene            348..420
                     /locus_tag="IVO87_002959"
     tRNA            348..420
                     /locus_tag="IVO87_002959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Lys,seq:ttt)
     gene            428..509
                     /locus_tag="IVO87_002960"
     tRNA            428..509
                     /locus_tag="IVO87_002960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:462..464,aa:Leu,seq:tag)
     gene            527..599
                     /locus_tag="IVO87_002961"
     tRNA            527..599
                     /locus_tag="IVO87_002961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Thr,seq:tgt)
     gene            611..682
                     /locus_tag="IVO87_002962"
     tRNA            611..682
                     /locus_tag="IVO87_002962"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Gly,seq:gcc)
     gene            699..784
                     /locus_tag="IVO87_002963"
     tRNA            699..784
                     /locus_tag="IVO87_002963"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:733..735,aa:Leu,seq:taa)
BASE COUNT          209 a          183 c          219 g          199 t
ORIGIN      
        1 agtgatgtat ggagcggact gatactaatc gatcgaggac ttaaccaata ggttaaaaga
       61 aaagaactcg gaaaaaaact tatgtgactt caaatccagt tttgagtgaa caaaatttac
      121 tcaataaaag ataacaccac agtgtggtgg cgatagcgag aaggatacac ctgtaaccat
      181 gccgaacaca gaagttaagc ttcttagcgc cgattgtagt gaggggttgc cccttgtgag
      241 agtaggacgt cgccacgctt aagttatcca tggaggttta gctcagctgg gagagcatct
      301 gccttacaag cagagggtca gcggttcgat cccgttaacc tccatatgag ccgttagctc
      361 agttggtaga gcatctgact tttaatcaga gggtcacagg ttcgagccct gtacggctca
      421 tatttttgcg ggtgtggcgg aattggcaga cgcactagat ttaggatcta gcgccttacg
      481 gcgtgggggt tcaagtccct tcacccgcat aatttaagtt ttacatgccg gcttagctca
      541 gttggtagag catctgattt gtaatcagag ggtcgagggt tcaagtcctt tagccggcat
      601 ctttaaacac gcggaaatag ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg
      661 ggttcgaacc ccgttttccg ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag
      721 acgcacagga cttaaaatcc tgcggtgagt gatcaccgta ccggttcgat tccggtcctc
      781 ggcatttcaa atatgcgccc atagctcaac
//
LOCUS       DACCQP010000202          794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCQP010000202 DACCQP010000000
VERSION     DACCQP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.211"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>794)
                     /locus_tag="IVO87_002964"
     CDS             complement(<1..>794)
                     /locus_tag="IVO87_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451067.1"
                     /translation="GGEISDLINDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTP
                     DKNTARNVLQINNAGIGFSKTGINGTYDTAWTLDGGFNASFITAGEIVGITIRGTTLI
                     SDGTDYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQ
                     GNLVFSTWDNGNNRPFLSFGAPNFRYSNASYINDGDGNSLGIDGSAGNSWEFKVAGRT
                     MKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNST"
BASE COUNT          247 a          184 c          110 g          253 t
ORIGIN      
        1 gttgagtttt tagtacctct aacagtaaaa tctttatata cttgtaccgt tgattgttca
       61 aatctagcaa gtttcccatc ttcccatgaa ccaaaaaaac aacctggcaa cgttagcata
      121 ccgtcactag taaatttcat agtcctacca gctactttaa attcccatga attacctgca
      181 ctaccatcaa tacctaaaga attaccgtca ccatcattta tataactagc attactgtat
      241 ctgaaatttg gcgcaccaaa agataaaaac ggtctgttat taccattatc ccacgtacta
      301 aaaaccaagt taccctgtgg atttctaatc atgaaaccac caccagtttt catggtgtat
      361 gatacaatgc cggcatcagc acttacataa tcacgtgctt ctagctccat aatatctttg
      421 ttaacttttt ttgagtacca agtcattttg ccattagcta tacttgttct ataatcagtg
      481 ccatcactaa ttaatgtagt acctctaata gtaatcccta ctatctcacc agctgtaata
      541 aacgaggcat tgaatccgcc atctaacgtc catgcggtgt catacgttcc attaatgcca
      601 gttttagaga aaccaatacc agcgttgttg atttgcaaaa cgttccgtgc agtattctta
      661 tctggtgtgt ccataatcaa aatacgacta ggggcttctt taggatctaa taaaacgtaa
      721 ccaccatttt gaccagtaat catatcagtt tgatgatcta caatatcatt gattaaatca
      781 ctgatttcgc cacc
//
LOCUS       DACCQP010000203          794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCQP010000203 DACCQP010000000
VERSION     DACCQP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.212"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            65..>794
                     /locus_tag="IVO87_002965"
     CDS             65..>794
                     /locus_tag="IVO87_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP7451068.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          290 a          148 c          132 g          224 t
ORIGIN      
        1 gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact
       61 acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga
      121 aattatgaga actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg
      181 aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg
      241 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
      301 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      361 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      421 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      481 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      541 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      601 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      661 cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat
      721 tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag accacagctt
      781 acttggaaaa caaa
//
LOCUS       DACCQP010000204          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCQP010000204 DACCQP010000000
VERSION     DACCQP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.213"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IVO87_002966"
     CDS             <1..782
                     /locus_tag="IVO87_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7451069.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DACCQP010000205          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCQP010000205 DACCQP010000000
VERSION     DACCQP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.214"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..581)
                     /locus_tag="IVO87_002967"
     CDS             complement(<1..581)
                     /locus_tag="IVO87_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451070.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
     gene            complement(591..>788)
                     /locus_tag="IVO87_002968"
     CDS             complement(591..>788)
                     /locus_tag="IVO87_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7451071.1"
                     /translation="EGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQYPTLQV
                     DINHFNCTGLSKIQIYRNGLR"
BASE COUNT          230 a          167 c          111 g          280 t
ORIGIN      
        1 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
      181 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgttt
      421 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaatgatt aatatccact
      661 tgcaacgttg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacatcagt tcctgctact
      781 aagccttc
//
LOCUS       DACCQP010000206          763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCQP010000206 DACCQP010000000
VERSION     DACCQP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.215"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(237..761)
                     /locus_tag="IVO87_002969"
     CDS             complement(237..761)
                     /locus_tag="IVO87_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451072.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          260 a          102 c           85 g          316 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DACCQP010000207          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCQP010000207 DACCQP010000000
VERSION     DACCQP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.216"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(397..711)
                     /locus_tag="IVO87_002970"
     CDS             complement(397..711)
                     /locus_tag="IVO87_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451073.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          279 a          102 c           94 g          243 t
ORIGIN      
        1 ttgttaaatc aacgtttttt tattttggtc aaggagagaa aagtagagaa atacaaaatt
       61 ttttgcattg attttgcatt caaaaaaaca ttcaaacatt tagaaatccc caatacgtat
      121 tcaggaaaat ctattttaag gtttacaaaa agaacttttg ttcgtacact ctttttgagg
      181 tgaactttta tgctgatgga aggaaaaaca caattatggt ttaagcatgt tccttcaaat
      241 aagtttttta attataaact ctagagaaat taattcatcc taacataaaa taattgttca
      301 ctcaaaagtt tttctagaac aattaaaaca gcaggcttca cattcgctat acatttgtaa
      361 gaagtaataa ttcctgtact aaaaggcata attgctttaa ttattttttg acttctcttc
      421 aaacacaaga agtagttgtg taataattgc agaaataagg attccaataa tagatataaa
      481 atttgacatg gaaacgtcta taacattaaa agaataaagt gttgaagaaa gtaaactgat
      541 tgaaataacc cctccgtaaa aaatagtctg tgaatatacc cacttttttt cagaagacgt
      601 acttcgtttg gttctaaaac ctatccctgc atttctactc ttatcaaaaa accaaatatt
      661 tagaagtaat aatattgcta ttattaagat aaaattactg taaaaatcca aaagctat
//
LOCUS       DACCQP010000208          604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCQP010000208 DACCQP010000000
VERSION     DACCQP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.217"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(14..427)
                     /locus_tag="IVO87_002971"
     CDS             complement(14..427)
                     /locus_tag="IVO87_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7451074.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          213 a           76 c           69 g          246 t
ORIGIN      
        1 attaaataaa tttttagtta ttaacttgat taaaatcaga gataatatca tttaattcag
       61 tttgaagggt tgtaacgatt aaatggaaat tttcttcatc tgttttatta tatggggatg
      121 ttttcgttat atataacaac ttttcttttt caattttcat taataactct gtcgaagtct
      181 gtactttatc ttgataatta aatgcagaca taccactttg tataattgta attattgctg
      241 aagcaattga aacaatcaat agtaaagacc tgtgttcgga agcctgatca attaatattg
      301 ggatactagc tgataagact atttttataa tatttccgat accaattact ctattataaa
      361 atcgaatttc tttttttaat ctatcgattg tagaatcaat agattcgata aacgcttttt
      421 gatccatcat tttctctcca atcattttat taaaatcatt aacaaaaata ttataatagg
      481 atttaagtaa gttctaacta atcacatata aaaataaaaa aacatttgta ttttatcaaa
      541 gttttattat acttgtcaat ttgtgtacat cctaaaatat aagctatttg tttacttctg
      601 taga
//
LOCUS       DACCQP010000209          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCQP010000209 DACCQP010000000
VERSION     DACCQP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.218"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            80..>576
                     /locus_tag="IVO87_002972"
     CDS             80..>576
                     /locus_tag="IVO87_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7451075.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          217 a          106 c           93 g          160 t
ORIGIN      
        1 aaaaatccat acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca
       61 cagaaaagag gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac
      121 taaaggcgat ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct
      181 acggactgaa ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc
      241 aggtaattcg agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac
      301 attggaaata cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa
      361 aagaacaaac gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat
      421 gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat
      481 gtccaacatg actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa
      541 tgataagtat gtcgctattt ttatggacgc tactta
//
LOCUS       DACCQP010000210          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCQP010000210 DACCQP010000000
VERSION     DACCQP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.219"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DACCQP010000211          535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCQP010000211 DACCQP010000000
VERSION     DACCQP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.220"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>535
                     /locus_tag="IVO87_002973"
     rRNA            <1..>535
                     /locus_tag="IVO87_002973"
                     /product="16S ribosomal RNA"
BASE COUNT          130 a          127 c          160 g          118 t
ORIGIN      
        1 tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag
       61 gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat
      121 gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcactc
      181 cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag
      241 cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc
      301 tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt ggtgcatggt
      361 tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt
      421 gttagttgcc atcattcagt tgggcactct agcaagactg ccggtgacaa accggaggaa
      481 ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgct
//
LOCUS       DACCQP010000212          535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCQP010000212 DACCQP010000000
VERSION     DACCQP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.221"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>535
                     /locus_tag="IVO87_002974"
     CDS             <1..>535
                     /locus_tag="IVO87_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP7451076.1"
                     /translation="DDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLP
                     EDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTT
                     ELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDV
                     TVTDNRSDADKYEPTVEG"
BASE COUNT          212 a           82 c          125 g          116 t
ORIGIN      
        1 tggatgatat cattgaaaac ccaggggata acattccggc tggctatcat aaagtaacct
       61 ttactgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
      121 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
      181 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      241 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      301 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      361 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      421 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      481 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DACCQP010000213          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCQP010000213 DACCQP010000000
VERSION     DACCQP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.222"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IVO87_002975"
     CDS             complement(<1..223)
                     /locus_tag="IVO87_002975"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7451077.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIM"
     gene            complement(334..>530)
                     /locus_tag="IVO87_002976"
     CDS             complement(334..>530)
                     /locus_tag="IVO87_002976"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7451078.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT          156 a           96 c           77 g          201 t
ORIGIN      
        1 ccatgatcgg tttattagac ctttgaggtc tatatttttt aactacaatc gaccttaaat
       61 ttaattgttt cattagcttt tggacatgtt taagagatac tgaaatccct tcttttaata
      121 aaacttgatg gatctttgtg gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa
      181 tcttcttttt caacttatta tttctgattt ctgtagctgt caacggttta ttcttataaa
      241 aataatagac acttctaggg atttctaata accgacacaa caaagaaaca gtgtaatcct
      301 tacaccattt ttcaataaat tgaacaagcg aatttaggtt ttccgagaga atatggctgc
      361 ggcttttttt aagatttcaa gttcctcatt taaacgagca ttttcttttc tcagttcagc
      421 tacttctttt ccagtcagtc ctgtggactg atcaggtaaa tataaattct tccatttgta
      481 aatcgttgct tcagataagc catattcttt cgctaaccct cgaacagaac
//
LOCUS       DACCQP010000214          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCQP010000214 DACCQP010000000
VERSION     DACCQP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.223"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            217..>517
                     /locus_tag="IVO87_002977"
     CDS             217..>517
                     /locus_tag="IVO87_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451079.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKI"
BASE COUNT          156 a           78 c           73 g          210 t
ORIGIN      
        1 tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat taattttgaa
       61 acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac tctatatcct
      121 ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt tctttatatt
      181 gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac caaaaaacat
      241 gttactagct cctttttcgc tattatatat acaacatctt cttttaggag tgtagaaata
      301 tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct tattttactc
      361 gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc gattagcatg
      421 actttattaa tttacactca tgttctttct gtagctatga ctatggtaat gattataatt
      481 gctttaatta ttcaatttta tcggaaaaag aaaatag
//
LOCUS       DACCQP010000215          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCQP010000215 DACCQP010000000
VERSION     DACCQP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.224"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVO87_002978"
     CDS             complement(<1..132)
                     /locus_tag="IVO87_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7451080.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCQP010000216          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCQP010000216 DACCQP010000000
VERSION     DACCQP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.225"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            122..>506
                     /locus_tag="IVO87_002979"
     CDS             122..>506
                     /locus_tag="IVO87_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451081.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQ"
BASE COUNT          179 a           79 c          105 g          143 t
ORIGIN      
        1 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
       61 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
      121 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
      181 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
      241 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
      301 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
      361 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
      421 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
      481 aattacagaa cttctgattc gtcaat
//
LOCUS       DACCQP010000217          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCQP010000217 DACCQP010000000
VERSION     DACCQP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.226"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>487)
                     /locus_tag="IVO87_002980"
     CDS             complement(<1..>487)
                     /locus_tag="IVO87_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7451082.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACCQP010000218          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCQP010000218 DACCQP010000000
VERSION     DACCQP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.227"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(72..>479)
                     /locus_tag="IVO87_002981"
     CDS             complement(72..>479)
                     /locus_tag="IVO87_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7451083.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          151 a           96 c           72 g          160 t
ORIGIN      
        1 acacgaccta aagatcgtgt ctaattatga ctgtatcaga aaatatgata ccaaacggat
       61 ctattcatta attaattgac atagtttatc tgttgctctt ttatgactgg ataactgacc
      121 aattcatgac gatccaagtc ttctttattc atatttacag cagtaatttg actgtcttca
      181 tacgtacgat aataataaat ccctttatca acgttacagc aagaagaata aatcgtatat
      241 tcgtattttc cttctccagt atcgcatact cctttttgtt gctcgactga gcctagtata
      301 tgaaaaaatt gactgatact ttcagattct gaatcacctg atgcagcatt cattttagta
      361 aatgtggctt ttacaaatcg agaaacagaa gagagatctc caggtaagcc caatcctccc
      421 atcccacgac tataaacatt caagctgatc tggtttgaaa aatgattttc tggtgttcc
//
LOCUS       DACCQP010000219          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCQP010000219 DACCQP010000000
VERSION     DACCQP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.228"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IVO87_002982"
     CDS             complement(<1..430)
                     /locus_tag="IVO87_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7451084.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCQP010000220          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCQP010000220 DACCQP010000000
VERSION     DACCQP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.229"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>457)
                     /locus_tag="IVO87_002983"
     rRNA            complement(<1..>457)
                     /locus_tag="IVO87_002983"
                     /product="16S ribosomal RNA"
BASE COUNT           92 a          147 c          102 g          116 t
ORIGIN      
        1 actcaagtct cccagtttcc aatgaccctc cccggttgag ccgggggctt tcacatcaga
       61 cttaagaaac cgcctgcgct cgctttacgc ccaataaatc cggacaacgc ttgccaccta
      121 cgtattaccg cggctgctgg cacgtagtta gccgtggctt tctggttaga taccgtcaag
      181 ggatgaacag ttactctcat ccttgttctt ctctaacaac agagttttac gatccgaaaa
      241 ccttcttcac tcacgcggcg ttgctcggtc agactttcgt ccattgccga agattcccta
      301 ctgctgcctc ccgtaggagt ttgggccgtg tctcagtccc aatgtggccg atcaccctct
      361 caggtcggct atgcatcgtg gccttggtga gccgttacct caccaactag ctaatgcacc
      421 gcgggtccat ccatcagcga cacccgaaag cgccttt
//
LOCUS       DACCQP010000221          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCQP010000221 DACCQP010000000
VERSION     DACCQP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.230"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..421
                     /locus_tag="IVO87_002984"
     CDS             <1..421
                     /locus_tag="IVO87_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7451085.1"
                     /translation="SRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWK
                     KNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEES
                     LERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          172 a           82 c           70 g          128 t
ORIGIN      
        1 atctcgtaac atcgctcata aagtacgtgt caaagaccga aaagaaatct gtgatgactt
       61 taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat ccggcatgat
      121 tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa accaagactt
      181 attaactttt tatgattttc cacctagcat tcgtagaacc atttactcaa ccaatctaat
      241 cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt ttcaaaatga
      301 agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa aatttctaaa
      361 cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt ttactgagta
      421 actaattatt ttgcaggagg acaatttatt ta
//
LOCUS       DACCQP010000222          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCQP010000222 DACCQP010000000
VERSION     DACCQP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.231"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           87 c           52 g          169 t
ORIGIN      
        1 ataaaaaaat tattacgtta ttaaaaaaac agatgaaaga tcaggtttta ttagatctca
       61 ttctttcatc acttgaaggg atttaatgaa ctactttttc ctctttgtcg tttttatata
      121 ttcgtattcc ttcaaacagt aggatagcct ctttcaatgc ttgatatttg aactgtccgt
      181 tacttaaaaa tttagatgtg ccgccctctt cctagaacat attctttttt actttttcat
      241 taattgacag accaatcgaa cgaaactgta aatttcgata ggataatgat tggtcaaact
      301 cattcatttt caagacatct cgattcatat tcaagatagg aatcttttta tcaacatcac
      361 tttattcgta ttttcctcta ccataaaagc ttcacgctcc acaaaattgt tttatacact
      421 actattcagc ccattcaacg accaatgaat t
//
LOCUS       DACCQP010000223          436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCQP010000223 DACCQP010000000
VERSION     DACCQP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.232"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>436
                     /locus_tag="IVO87_002985"
     CDS             <1..>436
                     /locus_tag="IVO87_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAP7451086.1"
                     /translation="TDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTTPMTTTIKAGE
                     TIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPTGEIVQEMTTD
                     ENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELK"
BASE COUNT          154 a           88 c           96 g           98 t
ORIGIN      
        1 actgacaaag aaggcattgc tacattggat gagattcctc atggtacaaa agtaaccatt
       61 accgaaaaat cagtaccggc gccttatacg attgatacca cccccatgac taccaccatt
      121 aaagcaggcg aaaccattta cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt
      181 ctggataaaa gcggagttga aacagggagt gatctttgga atgacaatta ttccttagct
      241 ggtaatacgt ttgccattcg caaagacagt ccaaccggtg aaatcgttca agaaatgacc
      301 acggatgaaa acggtcatgc ggaaacacca aaagagattg ctaatgcgtt ggaattggga
      361 acctattatg tgaccgaaac taaagctagt catgggttcg tgaatacgtt caaacctgta
      421 aaagtcgaat tgaagt
//
LOCUS       DACCQP010000224          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCQP010000224 DACCQP010000000
VERSION     DACCQP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.233"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IVO87_002986"
     CDS             <1..>404
                     /locus_tag="IVO87_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7451087.1"
                     /translation="RDRRFDVTLLINGLPIVQIELKQVTAKDGVYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNEKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT          136 a           72 c           76 g          120 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
       61 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
      121 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
      241 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
      301 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagt
//
LOCUS       DACCQP010000225          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCQP010000225 DACCQP010000000
VERSION     DACCQP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.234"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IVO87_002987"
     CDS             <1..>404
                     /locus_tag="IVO87_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7451088.1"
                     /translation="RDRRFDVTLLINGLPIIQIELKQVTAKDGFYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNKKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT          133 a           72 c           77 g          122 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttattg atcaatggct taccgattat ccaaatcgag
       61 ctaaaacaag taactgcaaa agatggtttt tatcaggcgt ttaatcaaat caaaaaatat
      121 gctgaagaag gcatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa cgactcggta ttttgcaaat gccttaccga aggatttgca tcctaagttt
      241 ttatttagtt ggcgaacgaa agataataaa aaggtagaaa acttatatga gttttgcaaa
      301 caagtgctaa atatcccaga tgcccatcgc ttgattgcgg attatacgat tgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagt
//
LOCUS       DACCQP010000226          397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCQP010000226 DACCQP010000000
VERSION     DACCQP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.235"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IVO87_002988"
     CDS             <1..382
                     /locus_tag="IVO87_002988"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7451089.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          159 a           75 c           68 g           95 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaagaa aaaatggctt aaccgtctct actaaaa
//
LOCUS       DACCQP010000227          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCQP010000227 DACCQP010000000
VERSION     DACCQP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.237"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            10..>375
                     /locus_tag="IVO87_002989"
     CDS             10..>375
                     /locus_tag="IVO87_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7451090.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKL"
BASE COUNT          144 a           61 c           80 g           90 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa
       61 cgaattgctt ttaattttaa gaacgcagga aatgcaatcg caacgaattg taatggacaa
      121 ttagatggcg aaacagaaat gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa
      181 tcaaaatcta aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt
      241 tatcgacgtt tcaatgggtt gaaacaagat ggacgtatta aaccaggcat ttactcttac
      301 ggtcctgatt ccgtaggcga agatttctca cttgctgcag agatcgtgga tgacttcgaa
      361 gaaaatagaa agtta
//
LOCUS       DACCQP010000228          372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCQP010000228 DACCQP010000000
VERSION     DACCQP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.denovo.238"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            10..>372
                     /locus_tag="IVO87_002990"
     CDS             10..>372
                     /locus_tag="IVO87_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7451091.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKL"
BASE COUNT          146 a           55 c           72 g           99 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aacaacagta acaacttttg acaatataag tattaaaaga
       61 atttctttta attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa tggacaatta
      121 gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg gttcaacaga agtaaaatcg
      181 aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat
      241 cgacgcttca atgggttgaa acaagatgaa cgtattaaat cgggaattta ctcttatggt
      301 cctgattccg taggggaaga tttctctctt gctgcagaga tcgtggatga tttcgaagaa
      361 aaaaataagc tg
//
LOCUS       DACCQP010000229        18720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.046, whole genome shotgun sequence.
ACCESSION   DACCQP010000229 DACCQP010000000
VERSION     DACCQP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.046"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..2603
                     /locus_tag="IVO87_002991"
     CDS             <1..2603
                     /locus_tag="IVO87_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7451092.1"
                     /translation="AIHEIAHAKLHAIDREAPAEEQAARPDRSTREVQAESVAYTVCQ
                     YYGLDTSDYSFGYVADWSSGRELSELKASLETIRATASELITKIDGHFAQLQQEREAR
                     QEQEAVTVDVTPEAAAPAQAEEEQAPALDPAAEPLVTVLWSESPQLKDGQQMPLHEAD
                     ALFARLDAAYPAEAGYDKTAFRIDCIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFY
                     AQDENWKKHVIATTGIEGWEQDKAHRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGE
                     TLTPEETAYLGALLSYVQECRPMLNQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQE
                     AAAAGMTVEEYAAAGYEAPQQDTFSIYQLRGGEETRDYRFEPYERLQAAGLAVDSANY
                     DLVYTAPLDKSTSLEDIYRTFNIDHPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGY
                     MEVPEFLRENPLRTAELSTEQNENMIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQ
                     EEPPEAAPPQTAVRYYPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQ
                     KKRVEPEYHEKIDSLLDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNA
                     ASDRNMEEFQYIQGLLDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQELMKAVNAY
                     YRKHGTLDGCPHLTERGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEEL
                     TRQKEAAYVGWEFDGGTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQ
                     RQLNDTTIRVADRIKCIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTEQAKQPPA
                     REKSKGLEVE"
     gene            2604..3548
                     /locus_tag="IVO87_002992"
     CDS             2604..3548
                     /locus_tag="IVO87_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451093.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            3552..3755
                     /locus_tag="IVO87_002993"
     CDS             3552..3755
                     /locus_tag="IVO87_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451094.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(4055..4291)
                     /locus_tag="IVO87_002994"
     CDS             complement(4055..4291)
                     /locus_tag="IVO87_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7451095.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(4474..4845)
                     /locus_tag="IVO87_002995"
     CDS             complement(4474..4845)
                     /locus_tag="IVO87_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451096.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(4842..6170)
                     /locus_tag="IVO87_002996"
     CDS             complement(4842..6170)
                     /locus_tag="IVO87_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7451097.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(6131..6460)
                     /locus_tag="IVO87_002997"
     CDS             complement(6131..6460)
                     /locus_tag="IVO87_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7451098.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(6718..7089)
                     /locus_tag="IVO87_002998"
     CDS             complement(6718..7089)
                     /locus_tag="IVO87_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7451099.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            7234..7419
                     /locus_tag="IVO87_002999"
     CDS             7234..7419
                     /locus_tag="IVO87_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7451100.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            7931..8149
                     /locus_tag="IVO87_003000"
     CDS             7931..8149
                     /locus_tag="IVO87_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451101.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            8419..9225
                     /locus_tag="IVO87_003001"
     CDS             8419..9225
                     /locus_tag="IVO87_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7451102.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9288..10259
                     /locus_tag="IVO87_003002"
     CDS             9288..10259
                     /locus_tag="IVO87_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7451103.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10252..11280
                     /gene="vanB"
                     /locus_tag="IVO87_003003"
     CDS             10252..11280
                     /gene="vanB"
                     /locus_tag="IVO87_003003"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7451104.1"
                     /translation="MNRIKVAIIFGGCSEEHDVAVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAVCMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            11286..11894
                     /locus_tag="IVO87_003004"
     CDS             11286..11894
                     /locus_tag="IVO87_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7451105.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            12482..12913
                     /locus_tag="IVO87_003005"
     CDS             12482..12913
                     /locus_tag="IVO87_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7451106.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            12920..13153
                     /locus_tag="IVO87_003006"
     CDS             12920..13153
                     /locus_tag="IVO87_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7451107.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13075..13317)
                     /locus_tag="IVO87_003007"
     CDS             complement(13075..13317)
                     /locus_tag="IVO87_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451108.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            13574..13777
                     /locus_tag="IVO87_003008"
     CDS             13574..13777
                     /locus_tag="IVO87_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7451109.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            13861..15054
                     /locus_tag="IVO87_003009"
     CDS             13861..15054
                     /locus_tag="IVO87_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7451110.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            15494..16465
                     /locus_tag="IVO87_003010"
     CDS             15494..16465
                     /locus_tag="IVO87_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7451111.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            16497..17894
                     /locus_tag="IVO87_003011"
     CDS             16497..17894
                     /locus_tag="IVO87_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7451112.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            18053..18676
                     /locus_tag="IVO87_003012"
     CDS             18053..18676
                     /locus_tag="IVO87_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451113.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDSDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5160 a         4301 c         4849 g         4410 t
ORIGIN      
        1 ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag gcccccgccg
       61 aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa agcgtggcct
      121 ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc tatgtggccg
      181 attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc atccgggcca
      241 cggccagcga attgataacc aagatcgacg gccattttgc ccagcttcag caggagcgcg
      301 aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca gcggccccgg
      361 cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg cttgtgactg
      421 tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg catgaggccg
      481 acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat gacaaaaccg
      541 cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg cggcaggatt
      601 ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa ttttacgcgc
      661 aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc tgggagcagg
      721 acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag ctgcattgca
      781 gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa accctgacgc
      841 cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc
      901 tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc gaccagagct
      961 tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc gcagccggta
     1021 tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac accttttcca
     1081 tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt
     1141 tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat acggcaccgc
     1201 tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac cacccggcag
     1261 attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag aatggacagg
     1321 acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt ttgcgggaaa
     1381 atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt gacggcatta
     1441 tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct tttgaggatg
     1501 tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc ccgcaaacgg
     1561 ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa gccatgagct
     1621 tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc gatcaggccg
     1681 tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa aagatagaca
     1741 gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc tatgagatta
     1801 ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta cgggcgaaag
     1861 aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt cagggcttgc
     1921 ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg caggccgttt
     1981 ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca
     2041 atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgacc gaaagaggca
     2101 ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt
     2161 cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc attgaggaat
     2221 tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc actgtggaga
     2281 tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat gctgctgtac
     2341 gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca tggcagcggc
     2401 agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg
     2461 gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc
     2521 gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat
     2581 caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc
     2641 tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag
     2701 acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt
     2761 cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac
     2821 agtacaacac atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg
     2881 tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc
     2941 cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt
     3001 atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg
     3061 tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg
     3121 cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg
     3181 tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga
     3241 aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc
     3301 actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata
     3361 aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg
     3421 acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg
     3481 acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg
     3541 aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca
     3601 atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg
     3661 atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact
     3721 atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg
     3781 gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac
     3841 ccggtattgt cggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc
     3901 cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct
     3961 gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc
     4021 aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg
     4081 gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct
     4141 tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat
     4201 aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt
     4261 catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc
     4321 tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt
     4381 ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga
     4441 ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca
     4501 gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc
     4561 gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt
     4621 cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc
     4681 ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc
     4741 gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt
     4801 ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat
     4861 tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc
     4921 tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat
     4981 cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt
     5041 agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt
     5101 gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa
     5161 atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg
     5221 gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg
     5281 tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc
     5341 ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc
     5401 cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc
     5461 tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc
     5521 tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc
     5581 atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct
     5641 ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg
     5701 cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg
     5761 gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata
     5821 tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc
     5881 acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc
     5941 tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg
     6001 cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag
     6061 caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca
     6121 agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca
     6181 gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct
     6241 ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt
     6301 cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa
     6361 agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca
     6421 cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt
     6481 caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag
     6541 gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa
     6601 caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc
     6661 aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta
     6721 cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc
     6781 ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg
     6841 cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca
     6901 ggctttaata agctgtatga caaccggcag actgggaatg gccccgtcgt tttcgatgtt
     6961 ggcaagatag cgccagtcaa tatttatttt ctctgccatt gctttgcgcg aaacgcgctt
     7021 tgcttgccgg gccgctttca catccgcgcc gaaagtttca aagccgggac aatcttcaat
     7081 attcgccata tcgcacctcc cacttacatt gtatatttca tactctgccc gtggaatgtt
     7141 ggtatatgca cctttcttaa agtttatatt tcacattttg tttcttttat tcttcgagca
     7201 aaagaattat aatgtaccct gtggaggtgt actatggact acatgacgtt gaaagaggca
     7261 ggcgagaaat ggggtgtgcc cccacgatgg ataaactact attgtgctgc gggacgcatc
     7321 cccggcgctg taaagatggc gacgatctgg ctcattccca aaactgcgga aaagccgatt
     7381 gacaggcgta caaagaaagg aaaggcggtt actgaatagt ataatgaaac cggcgcattt
     7441 tggatatggt tttgaagaga gagaaagcat ggtattttgt cacggaaaaa ctataagatg
     7501 atagatggca ggctgctcca gaccgataaa aagtattctc atctgaaaca gaaagaaaag
     7561 atttacacat ggatgtatga agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg
     7621 aaacaaagac gaggaagctg ctgctgtatc agccgtatat ggtagaattg aaaaggccgg
     7681 catctggatt ctttatggtg aagtctccag acattggata actgctggtc taatgcgcct
     7741 ttgagtatgg ggtgattgat tttaataccg gggaggtacg gctttttgag gaaaatattc
     7801 tgggatttac atttggcgat tattacattt cctgtaactg caaggacacg gatgatgaag
     7861 atatgaggaa tatagacttg ctgattttct actgtcttga acggtattag gactgaatct
     7921 gctgcgcgga gtgcggcaag agcgaccgga gggagtacac acaggccctg cctgtgtgaa
     7981 aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg
     8041 tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc agaatatccg
     8101 taaaggatat tctgatccat tgcttgccag tattatcggg atgttttgac aaccgctgta
     8161 atcgaaacat cccaaacatt aagaaggtgt taattattat tatcttgatt taattcgttt
     8221 aaaccaataa tatagataca tacaccatga aaagaagcga gtatcccaat gacggatgat
     8281 taaaatccga aaagaaatcg ctttgaaact acagggaaac tacagactgg ctacagggaa
     8341 agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca
     8401 aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa
     8461 cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc
     8521 acagtctgca cgctgttttt ggggtggaga ctggcttccg tattggaggc aacacagata
     8581 ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa
     8641 aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg
     8701 aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt
     8761 gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact
     8821 gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt
     8881 atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg
     8941 gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg
     9001 gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa
     9061 aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt
     9121 gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac
     9181 aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg
     9241 ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg agaaaaagtg
     9301 tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc
     9361 cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat
     9421 tggccgctgg caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa
     9481 ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc
     9541 acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc
     9601 cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac
     9661 ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac
     9721 tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa
     9781 ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag
     9841 attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc
     9901 tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg
     9961 catttttaat caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt
    10021 tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg
    10081 tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga
    10141 tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata
    10201 ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga
    10261 ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtggc ggtaaaatcc
    10321 gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt
    10381 acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt
    10441 ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc
    10501 gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag
    10561 gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt
    10621 caaagctccg cagtttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc
    10681 atcgccgttc ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt
    10741 acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta
    10801 aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc
    10861 ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat
    10921 gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat
    10981 caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt
    11041 ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga
    11101 tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat
    11161 gaggtcaata ccctgcccgg ttttacatcg tacagccgct acccacgtat ggtggccgcc
    11221 gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga
    11281 taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg
    11341 ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc
    11401 gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg
    11461 cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct
    11521 tcctgcgttg ggcagcgcaa ccggaggaca acctcacaaa agaaaaattt taccccaata
    11581 tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg
    11641 gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa
    11701 gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag
    11761 aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc
    11821 gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt
    11881 ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat
    11941 gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca
    12001 aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct
    12061 ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct
    12121 tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt
    12181 gccatgctct aatccacttc caccactcaa aaaaagcctt gcccctccac agggctattc
    12241 cggttatgag tatgtactat accatgtaaa tcagcagcat agcaaagcat tttctcgccc
    12301 ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt gcggcccggc tgtccccaaa
    12361 aaaatttttt gagaaatttt caaaaaggag gtatttagcg ggtagctggc ggccttgtca
    12421 aagtcttgtt agcggaaagg gagagaacca cctaagtccc ccacagaaag ggggtgagaa
    12481 gatggatgct atcccccgcg actatgaagc gcggtgcatg ttcgatgctt tttgcaagac
    12541 ggtactgcgt aacgaggcca aaagctactt ggctgaaatg aagcgccgcc gtgaccgtga
    12601 agtgtcacta agctctttgt cacaggcaga tttggacaag ctctgtacag tagatcacta
    12661 ccccagcgac agctttacgt tttcgtcgca tggctatgac ctacatatca acaatgagct
    12721 tgttgccgaa gcctttgccg cgctgcccag catggagcag agtattttaa tcctgcactg
    12781 tgttctggat atggcagatg gtgagatcgg cggccttgtg ggaatgtccc ggagtgccgt
    12841 tcagcggcac aggacaaata ccttatcgga gctgcggaaa cagctaaagg cgttgatgcc
    12901 gaagggaggt tagacgcgga tgaagaaccc gaactacaag ggcattgagc ttttgcccct
    12961 ttcggtgatc gaggccgccc gcgctgggga tgccggagcc gtagagcgcg ttttgcagta
    13021 ttacagcggt tacatgaaca agctgtgtac ccggacgctc tatgacgaaa acggctatcc
    13081 gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa atcaagctca tccacgctat
    13141 cgtcacgaac tgacgaaccg gccccggcag catgaaacgg cttacccttt ccccgtttcg
    13201 gtgttttccc ggtggcctga tcgcaccttg ataaatatgc ccgcaagata acggcggcgc
    13261 atcatagggc atacggacac attctgtctg tgccgagccg gacggctggt gcgccatgat
    13321 acttttccct acgggaaaag cggagcgagg aacaccacgc cgcaatgtga gcgtggcggc
    13381 ttgcgggcga cgacgtacag gcaggactat gatactccct tatagccaca gtccgagcgt
    13441 taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc acgcgggggt gaaagtcccg
    13501 tggagctgtg ccagcagccg tccgtctatg cccctaaatc acacaaacct ttgaatttgt
    13561 gaaagggggc tgcattatga accatgatgt gcctatttgg gaaaaataca cgctgacgat
    13621 tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa ttgcgtaagc tggccgagga
    13681 aaatccggcc tctggctggg tcattatgaa tggcaaccgc atccagatca aacgcaagca
    13741 gtttgagaaa ataattgaca ctttggacac aatctaatag tgaaaaaggg ccgcagatat
    13801 gttataataa tttataagca tatatgcggc tctttccgtc atggaaagga gcatgacgag
    13861 atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc ggtctggaga gagccagaga
    13921 aaagacggac gatatgctta caaatatgtt gatacctttg gaaaaccgca gtttgtttac
    13981 tcatggaagt tagtgcctac ggataagacc ccggcaggta aacgggagga tatttcactc
    14041 cgggaaaagg agaaagaaat ccagaaagac cttgacgacg ggatagacac catcggcaag
    14101 aaaatgaccg tctgccagct ctacgccaag cagatcaggc accggggcaa tgtgaagcac
    14161 aacaccaaac tgggccggga acggctgatg cgtattctgg aacaagatcg gctggggagc
    14221 tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat gggcgcttcg gatgaaagaa
    14281 aagggccttt cttataagac catcaataac gacaagcgtt ccctgaaagc ggctttttac
    14341 accgccatac aggacgattg catccgaaaa aacccgtttg acttccagct tagtgacgtt
    14401 ctggacgacg atacggaacc aaaggtgcct cttacaccag cacaggaaga aagttttctg
    14461 tcctttattc aaggtgataa ggtttaccaa aagcattatg acgcaatcgt tattctgctg
    14521 gggacagggc ttcgcatttc ggaactgtgc ggactgacgg acaaagattt ggattttgaa
    14581 aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata ccggcgtcgg ttactatatt
    14641 gacgaaccca agacccaaag cggcgtcaga aaaatcccca tgaatgagga agtgtatcag
    14701 gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc cgttcattat tgacgggtat
    14761 gccaatttcc tgtttctcaa acagaacggc tatcccatga cggcggtaga ttatggcggg
    14821 atgtttggca ggcttgtgaa gaagtacaac aagagccatg aagaagcgtt gccgaaaacg
    14881 acgacgcccc atgctatgcg tcatacattc tgcacccggt tagccaatgc gggaatgaac
    14941 cccaaagcct tgcagtacat tatgggacat tccaatatca ccatgacgct gaactattat
    15001 gcacacgcca cattcgattc cgcaagggca gaaatggaac ggctggcagc ctgacacaag
    15061 gccggtttta ctactccatt tactactttt gagggggaaa tggtgagagg taataagaac
    15121 atttgtaagt atctaacaat atcaaaaatg ccggaaaagc ccggaaacac gggctatacc
    15181 gacattcaag aacttctaaa aagataatct aaaatcccat ataattttag aataaaattt
    15241 taaaaaggca aaaaattcgc cattttagga aagcgtttta aaaaagtgtt gcaatctttt
    15301 aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt
    15361 gagaggaagt tgctctactg aagactttag ttatatggat attgatgaca agcaacacta
    15421 tgcaaagaaa gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca
    15481 ggagtgagaa tgtatggcat cgatccgtga cgtggctaag atggctggtg tttcagtggg
    15541 aacagtttcc cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc
    15601 agaagcgatt tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa
    15661 tcgaagcttc tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt
    15721 tgtctcaggg atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt
    15781 tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga
    15841 tgggctgatt gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat
    15901 gagtattcca gttatcgcat tgaattgccc agaaggacca gcaggtattg atacaatatt
    15961 agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa
    16021 aaaaatcgga tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg
    16081 agcaaaagaa gcggtacaaa atgatccatc gatccaatta gaggtcttta caggagacta
    16141 ttctagaaaa agtggctatt atggggcaaa agaattatta gaaaaaggag taacagcgat
    16201 ttttgtcagc aattataata tgtcgatcgg tgctatcgaa tgtttcaatc aagaaggtat
    16261 cagaatcggg aaggatattg cgtttagtca ttatgattat ttggataaaa acgatttgag
    16321 catccttccg aaaatcacga ttacaccacc aacagataaa atcggggaac aagccgcaga
    16381 acagctgttg aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtaa ttgtgatgga
    16441 aaatatcata catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga
    16501 aaaaatatca atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag
    16561 aaggaaggat ttcaggtgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag
    16621 aaccgcaaaa gttctttgac caagtaggac ctgaaaaaac ttcacaagtt tatacgaagt
    16681 atcaagagga tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc
    16741 aatggagccg cttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct
    16801 atcatcgtta ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc
    16861 atttcgatat gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg
    16921 aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt cggttcgaaa gtaaaaaaat
    16981 ggtttactca taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt
    17041 atccagaaga gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggcct
    17101 cagcgttagc catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct
    17161 tgaatctgac accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc
    17221 aaatagcaga tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc
    17281 caagtgaatt gattgagata gtaaaagaac ttgatctcat gcctgtgatc catccagagg
    17341 atttgacggt catcaaagaa aatacagtag atcttttagg tatcaattat taccagccac
    17401 gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt
    17461 atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct
    17521 atgaacaagg aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact
    17581 gctttatttc tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg
    17641 gcatgatcca cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc
    17701 aagcaattca agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact
    17761 ggtcatggac caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag
    17821 atggaaaaag aacaatcaaa aaatcgggtc actggttcaa agacgtatca gataaaaacg
    17881 gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca
    17941 ttttgtgaaa aagaagttca attatttacg atctttttca acttttttgt ctttctattt
    18001 cattttttgt atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa
    18061 aaaaagaccg tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc
    18121 agcattttca ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc
    18181 aagcaaagaa tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta
    18241 taaaataact gccaattacg aaaaaggcga attggacatc tatttgccaa aaggaaaaga
    18301 agaaaatcaa tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca
    18361 atttaaagac aagcatttga ccgttttgct tttgactcat gatagcgaca aagtagcccg
    18421 tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc
    18481 cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc
    18541 tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac
    18601 gggaagtgaa gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca
    18661 atcctatgga caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag
//
LOCUS       DACCQP010000230        18720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.047, whole genome shotgun sequence.
ACCESSION   DACCQP010000230 DACCQP010000000
VERSION     DACCQP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.047"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..2603
                     /locus_tag="IVO87_003013"
     CDS             <1..2603
                     /locus_tag="IVO87_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7451114.1"
                     /translation="AIHEIAHAKLHAIDREAPAEEQAARPDRSTREVQAESVAYTVCQ
                     YYGLDTSDYSFGYVADWSSGRELSELKASLETIRATASELITKIDGHFAQLQQEREAR
                     QEQEAVTVDVTPEAAAPAQAEEEQAPALDPAAEPLVTVLWSESPQLKDGQQMPLHEAD
                     ALFARLDAAYPAEAGYDKTAFRIDCIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFY
                     AQDENWKKHVIATTGIEGWEQDKAHRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGE
                     TLTPEETAYLGALLSYVQECRPMLNQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQE
                     AAAAGMTVEEYAAAGYEAPQQDTFSIYQLRGGEETRDYRFEPYERLQAAGLAVDSANY
                     DLVYTAPLDKSTSLEDIYRTFNIDHPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGY
                     MEVPEFLRENPLRTAELSTEQNENMIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQ
                     EEPPEAAPPQTAVRYYPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQ
                     KKRVEPEYHEKIDSLLDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNA
                     ASDRNMEEFQYIQGLLDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQELMKAVNAY
                     YRKHGTLDGCPHLTERGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEEL
                     TRQKEAAYVGWEFDGGTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQ
                     RQLNDTTIRVADRIKCIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTEQAKQPPA
                     REKSKGLEVE"
     gene            2604..3548
                     /locus_tag="IVO87_003014"
     CDS             2604..3548
                     /locus_tag="IVO87_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451115.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            3552..3755
                     /locus_tag="IVO87_003015"
     CDS             3552..3755
                     /locus_tag="IVO87_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451116.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(4055..4291)
                     /locus_tag="IVO87_003016"
     CDS             complement(4055..4291)
                     /locus_tag="IVO87_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7451117.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(4474..4845)
                     /locus_tag="IVO87_003017"
     CDS             complement(4474..4845)
                     /locus_tag="IVO87_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451118.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(4842..6170)
                     /locus_tag="IVO87_003018"
     CDS             complement(4842..6170)
                     /locus_tag="IVO87_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7451119.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(6131..6460)
                     /locus_tag="IVO87_003019"
     CDS             complement(6131..6460)
                     /locus_tag="IVO87_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7451120.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(6718..7089)
                     /locus_tag="IVO87_003020"
     CDS             complement(6718..7089)
                     /locus_tag="IVO87_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7451121.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            7234..7419
                     /locus_tag="IVO87_003021"
     CDS             7234..7419
                     /locus_tag="IVO87_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7451122.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            7931..8149
                     /locus_tag="IVO87_003022"
     CDS             7931..8149
                     /locus_tag="IVO87_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451123.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            8419..9225
                     /locus_tag="IVO87_003023"
     CDS             8419..9225
                     /locus_tag="IVO87_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7451124.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9288..10259
                     /locus_tag="IVO87_003024"
     CDS             9288..10259
                     /locus_tag="IVO87_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7451125.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10252..11280
                     /gene="vanB"
                     /locus_tag="IVO87_003025"
     CDS             10252..11280
                     /gene="vanB"
                     /locus_tag="IVO87_003025"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7451126.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAVCMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            11286..11894
                     /locus_tag="IVO87_003026"
     CDS             11286..11894
                     /locus_tag="IVO87_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7451127.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            12482..12913
                     /locus_tag="IVO87_003027"
     CDS             12482..12913
                     /locus_tag="IVO87_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7451128.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            12920..13153
                     /locus_tag="IVO87_003028"
     CDS             12920..13153
                     /locus_tag="IVO87_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7451129.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13075..13317)
                     /locus_tag="IVO87_003029"
     CDS             complement(13075..13317)
                     /locus_tag="IVO87_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451130.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            13574..13777
                     /locus_tag="IVO87_003030"
     CDS             13574..13777
                     /locus_tag="IVO87_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7451131.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            13861..15054
                     /locus_tag="IVO87_003031"
     CDS             13861..15054
                     /locus_tag="IVO87_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7451132.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            15494..16465
                     /locus_tag="IVO87_003032"
     CDS             15494..16465
                     /locus_tag="IVO87_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7451133.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            16497..17894
                     /locus_tag="IVO87_003033"
     CDS             16497..17894
                     /locus_tag="IVO87_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7451134.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            18053..18676
                     /locus_tag="IVO87_003034"
     CDS             18053..18676
                     /locus_tag="IVO87_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451135.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDSDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5160 a         4301 c         4848 g         4411 t
ORIGIN      
        1 ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag gcccccgccg
       61 aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa agcgtggcct
      121 ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc tatgtggccg
      181 attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc atccgggcca
      241 cggccagcga attgataacc aagatcgacg gccattttgc ccagcttcag caggagcgcg
      301 aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca gcggccccgg
      361 cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg cttgtgactg
      421 tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg catgaggccg
      481 acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat gacaaaaccg
      541 cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg cggcaggatt
      601 ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa ttttacgcgc
      661 aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc tgggagcagg
      721 acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag ctgcattgca
      781 gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa accctgacgc
      841 cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc
      901 tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc gaccagagct
      961 tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc gcagccggta
     1021 tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac accttttcca
     1081 tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt
     1141 tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat acggcaccgc
     1201 tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac cacccggcag
     1261 attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag aatggacagg
     1321 acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt ttgcgggaaa
     1381 atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt gacggcatta
     1441 tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct tttgaggatg
     1501 tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc ccgcaaacgg
     1561 ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa gccatgagct
     1621 tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc gatcaggccg
     1681 tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa aagatagaca
     1741 gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc tatgagatta
     1801 ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta cgggcgaaag
     1861 aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt cagggcttgc
     1921 ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg caggccgttt
     1981 ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca
     2041 atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgacc gaaagaggca
     2101 ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt
     2161 cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc attgaggaat
     2221 tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc actgtggaga
     2281 tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat gctgctgtac
     2341 gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca tggcagcggc
     2401 agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg
     2461 gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc
     2521 gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat
     2581 caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc
     2641 tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag
     2701 acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt
     2761 cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac
     2821 agtacaacac atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg
     2881 tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc
     2941 cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt
     3001 atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg
     3061 tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg
     3121 cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg
     3181 tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga
     3241 aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc
     3301 actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata
     3361 aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg
     3421 acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg
     3481 acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg
     3541 aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca
     3601 atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg
     3661 atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact
     3721 atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg
     3781 gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac
     3841 ccggtattgt cggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc
     3901 cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct
     3961 gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc
     4021 aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg
     4081 gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct
     4141 tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat
     4201 aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt
     4261 catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc
     4321 tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt
     4381 ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga
     4441 ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca
     4501 gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc
     4561 gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt
     4621 cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc
     4681 ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc
     4741 gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt
     4801 ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat
     4861 tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc
     4921 tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat
     4981 cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt
     5041 agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt
     5101 gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa
     5161 atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg
     5221 gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg
     5281 tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc
     5341 ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc
     5401 cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc
     5461 tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc
     5521 tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc
     5581 atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct
     5641 ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg
     5701 cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg
     5761 gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata
     5821 tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc
     5881 acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc
     5941 tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg
     6001 cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag
     6061 caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca
     6121 agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca
     6181 gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct
     6241 ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt
     6301 cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa
     6361 agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca
     6421 cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt
     6481 caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag
     6541 gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa
     6601 caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc
     6661 aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta
     6721 cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc
     6781 ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg
     6841 cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca
     6901 ggctttaata agctgtatga caaccggcag actgggaatg gccccgtcgt tttcgatgtt
     6961 ggcaagatag cgccagtcaa tatttatttt ctctgccatt gctttgcgcg aaacgcgctt
     7021 tgcttgccgg gccgctttca catccgcgcc gaaagtttca aagccgggac aatcttcaat
     7081 attcgccata tcgcacctcc cacttacatt gtatatttca tactctgccc gtggaatgtt
     7141 ggtatatgca cctttcttaa agtttatatt tcacattttg tttcttttat tcttcgagca
     7201 aaagaattat aatgtaccct gtggaggtgt actatggact acatgacgtt gaaagaggca
     7261 ggcgagaaat ggggtgtgcc cccacgatgg ataaactact attgtgctgc gggacgcatc
     7321 cccggcgctg taaagatggc gacgatctgg ctcattccca aaactgcgga aaagccgatt
     7381 gacaggcgta caaagaaagg aaaggcggtt actgaatagt ataatgaaac cggcgcattt
     7441 tggatatggt tttgaagaga gagaaagcat ggtattttgt cacggaaaaa ctataagatg
     7501 atagatggca ggctgctcca gaccgataaa aagtattctc atctgaaaca gaaagaaaag
     7561 atttacacat ggatgtatga agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg
     7621 aaacaaagac gaggaagctg ctgctgtatc agccgtatat ggtagaattg aaaaggccgg
     7681 catctggatt ctttatggtg aagtctccag acattggata actgctggtc taatgcgcct
     7741 ttgagtatgg ggtgattgat tttaataccg gggaggtacg gctttttgag gaaaatattc
     7801 tgggatttac atttggcgat tattacattt cctgtaactg caaggacacg gatgatgaag
     7861 atatgaggaa tatagacttg ctgattttct actgtcttga acggtattag gactgaatct
     7921 gctgcgcgga gtgcggcaag agcgaccgga gggagtacac acaggccctg cctgtgtgaa
     7981 aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg
     8041 tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc agaatatccg
     8101 taaaggatat tctgatccat tgcttgccag tattatcggg atgttttgac aaccgctgta
     8161 atcgaaacat cccaaacatt aagaaggtgt taattattat tatcttgatt taattcgttt
     8221 aaaccaataa tatagataca tacaccatga aaagaagcga gtatcccaat gacggatgat
     8281 taaaatccga aaagaaatcg ctttgaaact acagggaaac tacagactgg ctacagggaa
     8341 agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca
     8401 aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa
     8461 cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc
     8521 acagtctgca cgctgttttt ggggtggaga ctggcttccg tattggaggc aacacagata
     8581 ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa
     8641 aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg
     8701 aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt
     8761 gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact
     8821 gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt
     8881 atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg
     8941 gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg
     9001 gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa
     9061 aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt
     9121 gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac
     9181 aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg
     9241 ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg agaaaaagtg
     9301 tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc
     9361 cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat
     9421 tggccgctgg caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa
     9481 ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc
     9541 acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc
     9601 cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac
     9661 ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac
     9721 tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa
     9781 ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag
     9841 attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc
     9901 tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg
     9961 catttttaat caacaccggg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt
    10021 tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg
    10081 tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga
    10141 tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata
    10201 ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga
    10261 ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc
    10321 gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt
    10381 acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt
    10441 ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc
    10501 gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag
    10561 gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt
    10621 caaagctccg cagtttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc
    10681 atcgccgttc ccgaatttca aatgattgat aaaggtgaca agccggaggc gggtgcgctt
    10741 acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta
    10801 aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc
    10861 ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat
    10921 gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat
    10981 caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt
    11041 ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga
    11101 tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat
    11161 gaggtcaata ccctgcccgg ttttacatcg tacagccgct acccacgtat ggtggccgcc
    11221 gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga
    11281 taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg
    11341 ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc
    11401 gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg
    11461 cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct
    11521 tcctgcgttg ggcagcgcaa ccggaggaca acctcacaaa agaaaaattt taccccaata
    11581 tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg
    11641 gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa
    11701 gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag
    11761 aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc
    11821 gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt
    11881 ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat
    11941 gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca
    12001 aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct
    12061 ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct
    12121 tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt
    12181 gccatgctct aatccacttc caccactcaa aaaaagcctt gcccctccac agggctattc
    12241 cggttatgag tatgtactat accatgtaaa tcagcagcat agcaaagcat tttctcgccc
    12301 ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt gcggcccggc tgtccccaaa
    12361 aaaatttttt gagaaatttt caaaaaggag gtatttagcg ggtagctggc ggccttgtca
    12421 aagtcttgtt agcggaaagg gagagaacca cctaagtccc ccacagaaag ggggtgagaa
    12481 gatggatgct atcccccgcg actatgaagc gcggtgcatg ttcgatgctt tttgcaagac
    12541 ggtactgcgt aacgaggcca aaagctactt ggctgaaatg aagcgccgcc gtgaccgtga
    12601 agtgtcacta agctctttgt cacaggcaga tttggacaag ctctgtacag tagatcacta
    12661 ccccagcgac agctttacgt tttcgtcgca tggctatgac ctacatatca acaatgagct
    12721 tgttgccgaa gcctttgccg cgctgcccag catggagcag agtattttaa tcctgcactg
    12781 tgttctggat atggcagatg gtgagatcgg cggccttgtg ggaatgtccc ggagtgccgt
    12841 tcagcggcac aggacaaata ccttatcgga gctgcggaaa cagctaaagg cgttgatgcc
    12901 gaagggaggt tagacgcgga tgaagaaccc gaactacaag ggcattgagc ttttgcccct
    12961 ttcggtgatc gaggccgccc gcgctgggga tgccggagcc gtagagcgcg ttttgcagta
    13021 ttacagcggt tacatgaaca agctgtgtac ccggacgctc tatgacgaaa acggctatcc
    13081 gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa atcaagctca tccacgctat
    13141 cgtcacgaac tgacgaaccg gccccggcag catgaaacgg cttacccttt ccccgtttcg
    13201 gtgttttccc ggtggcctga tcgcaccttg ataaatatgc ccgcaagata acggcggcgc
    13261 atcatagggc atacggacac attctgtctg tgccgagccg gacggctggt gcgccatgat
    13321 acttttccct acgggaaaag cggagcgagg aacaccacgc cgcaatgtga gcgtggcggc
    13381 ttgcgggcga cgacgtacag gcaggactat gatactccct tatagccaca gtccgagcgt
    13441 taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc acgcgggggt gaaagtcccg
    13501 tggagctgtg ccagcagccg tccgtctatg cccctaaatc acacaaacct ttgaatttgt
    13561 gaaagggggc tgcattatga accatgatgt gcctatttgg gaaaaataca cgctgacgat
    13621 tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa ttgcgtaagc tggccgagga
    13681 aaatccggcc tctggctggg tcattatgaa tggcaaccgc atccagatca aacgcaagca
    13741 gtttgagaaa ataattgaca ctttggacac aatctaatag tgaaaaaggg ccgcagatat
    13801 gttataataa tttataagca tatatgcggc tctttccgtc atggaaagga gcatgacgag
    13861 atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc ggtctggaga gagccagaga
    13921 aaagacggac gatatgctta caaatatgtt gatacctttg gaaaaccgca gtttgtttac
    13981 tcatggaagt tagtgcctac ggataagacc ccggcaggta aacgggagga tatttcactc
    14041 cgggaaaagg agaaagaaat ccagaaagac cttgacgacg ggatagacac catcggcaag
    14101 aaaatgaccg tctgccagct ctacgccaag cagatcaggc accggggcaa tgtgaagcac
    14161 aacaccaaac tgggccggga acggctgatg cgtattctgg aacaagatcg gctggggagc
    14221 tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat gggcgcttcg gatgaaagaa
    14281 aagggccttt cttataagac catcaataac gacaagcgtt ccctgaaagc ggctttttac
    14341 accgccatac aggacgattg catccgaaaa aacccgtttg acttccagct tagtgacgtt
    14401 ctggacgacg atacggaacc aaaggtgcct cttacaccag cacaggaaga aagttttctg
    14461 tcctttattc aaggtgataa ggtttaccaa aagcattatg acgcaatcgt tattctgctg
    14521 gggacagggc ttcgcatttc ggaactgtgc ggactgacgg acaaagattt ggattttgaa
    14581 aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata ccggcgtcgg ttactatatt
    14641 gacgaaccca agacccaaag cggcgtcaga aaaatcccca tgaatgagga agtgtatcag
    14701 gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc cgttcattat tgacgggtat
    14761 gccaatttcc tgtttctcaa acagaacggc tatcccatga cggcggtaga ttatggcggg
    14821 atgtttggca ggcttgtgaa gaagtacaac aagagccatg aagaagcgtt gccgaaaacg
    14881 acgacgcccc atgctatgcg tcatacattc tgcacccggt tagccaatgc gggaatgaac
    14941 cccaaagcct tgcagtacat tatgggacat tccaatatca ccatgacgct gaactattat
    15001 gcacacgcca cattcgattc cgcaagggca gaaatggaac ggctggcagc ctgacacaag
    15061 gccggtttta ctactccatt tactactttt gagggggaaa tggtgagagg taataagaac
    15121 atttgtaagt atctaacaat atcaaaaatg ccggaaaagc ccggaaacac gggctatacc
    15181 gacattcaag aacttctaaa aagataatct aaaatcccat ataattttag aataaaattt
    15241 taaaaaggca aaaaattcgc cattttagga aagcgtttta aaaaagtgtt gcaatctttt
    15301 aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt
    15361 gagaggaagt tgctctactg aagactttag ttatatggat attgatgaca agcaacacta
    15421 tgcaaagaaa gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca
    15481 ggagtgagaa tgtatggcat cgatccgtga cgtggctaag atggctggtg tttcagtggg
    15541 aacagtttcc cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc
    15601 agaagcgatt tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa
    15661 tcgaagcttc tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt
    15721 tgtctcaggg atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt
    15781 tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga
    15841 tgggctgatt gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat
    15901 gagtattcca gttatcgcat tgaattgccc agaaggacca gcaggtattg atacaatatt
    15961 agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa
    16021 aaaaatcgga tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg
    16081 agcaaaagaa gcggtacaaa atgatccatc gatccaatta gaggtcttta caggagacta
    16141 ttctagaaaa agtggctatt atggggcaaa agaattatta gaaaaaggag taacagcgat
    16201 ttttgtcagc aattataata tgtcgatcgg tgctatcgaa tgtttcaatc aagaaggtat
    16261 cagaatcggg aaggatattg cgtttagtca ttatgattat ttggataaaa acgatttgag
    16321 catccttccg aaaatcacga ttacaccacc aacagataaa atcggggaac aagccgcaga
    16381 acagctgttg aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtaa ttgtgatgga
    16441 aaatatcata catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga
    16501 aaaaatatca atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag
    16561 aaggaaggat ttcaggtgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag
    16621 aaccgcaaaa gttctttgac caagtaggac ctgaaaaaac ttcacaagtt tatacgaagt
    16681 atcaagagga tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc
    16741 aatggagccg cttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct
    16801 atcatcgtta ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc
    16861 atttcgatat gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg
    16921 aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt cggttcgaaa gtaaaaaaat
    16981 ggtttactca taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt
    17041 atccagaaga gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggcct
    17101 cagcgttagc catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct
    17161 tgaatctgac accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc
    17221 aaatagcaga tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc
    17281 caagtgaatt gattgagata gtaaaagaac ttgatctcat gcctgtgatc catccagagg
    17341 atttgacggt catcaaagaa aatacagtag atcttttagg tatcaattat taccagccac
    17401 gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt
    17461 atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct
    17521 atgaacaagg aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact
    17581 gctttatttc tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg
    17641 gcatgatcca cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc
    17701 aagcaattca agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact
    17761 ggtcatggac caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag
    17821 atggaaaaag aacaatcaaa aaatcgggtc actggttcaa agacgtatca gataaaaacg
    17881 gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca
    17941 ttttgtgaaa aagaagttca attatttacg atctttttca acttttttgt ctttctattt
    18001 cattttttgt atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa
    18061 aaaaagaccg tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc
    18121 agcattttca ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc
    18181 aagcaaagaa tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta
    18241 taaaataact gccaattacg aaaaaggcga attggacatc tatttgccaa aaggaaaaga
    18301 agaaaatcaa tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca
    18361 atttaaagac aagcatttga ccgttttgct tttgactcat gatagcgaca aagtagcccg
    18421 tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc
    18481 cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc
    18541 tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac
    18601 gggaagtgaa gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca
    18661 atcctatgga caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag
//
LOCUS       DACCQP010000231        12144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.083, whole genome shotgun sequence.
ACCESSION   DACCQP010000231 DACCQP010000000
VERSION     DACCQP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.083"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1093)
                     /locus_tag="IVO87_003035"
     CDS             complement(<1..1093)
                     /locus_tag="IVO87_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002831727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7451136.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYF"
     gene            complement(1090..1962)
                     /locus_tag="IVO87_003036"
     CDS             complement(1090..1962)
                     /locus_tag="IVO87_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7451137.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(2304..3176)
                     /locus_tag="IVO87_003037"
     CDS             complement(2304..3176)
                     /locus_tag="IVO87_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7451138.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            3304..3873
                     /locus_tag="IVO87_003038"
     CDS             3304..3873
                     /locus_tag="IVO87_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7451139.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3940..4677
                     /locus_tag="IVO87_003039"
     CDS             3940..4677
                     /locus_tag="IVO87_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7451140.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            4908..6176
                     /locus_tag="IVO87_003040"
     CDS             4908..6176
                     /locus_tag="IVO87_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7451141.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            6663..7127
                     /locus_tag="IVO87_003041"
     CDS             6663..7127
                     /locus_tag="IVO87_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7451142.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            7111..7740
                     /locus_tag="IVO87_003042"
     CDS             7111..7740
                     /locus_tag="IVO87_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7451143.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            8094..9572
                     /gene="msr"
                     /locus_tag="IVO87_003043"
     CDS             8094..9572
                     /gene="msr"
                     /locus_tag="IVO87_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7451144.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            9619..10065
                     /locus_tag="IVO87_003044"
     CDS             9619..10065
                     /locus_tag="IVO87_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7451145.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(10264..10779)
                     /locus_tag="IVO87_003045"
     CDS             complement(10264..10779)
                     /locus_tag="IVO87_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7451146.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            10896..12098
                     /locus_tag="IVO87_003046"
     CDS             10896..12098
                     /locus_tag="IVO87_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7451147.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3848 a         2211 c         2473 g         3612 t
ORIGIN      
        1 caaagtagta aatcactgct gttactaatc cttggacgat caacatcaat ggctgattcg
       61 caatcggatt tttcaaagat aggaagtaat cgacaaatcc ggcactaaag gcaaatcctg
      121 ctgtccaatg gaagaatgca ctcactgcta aagaaatccc agtgaaaatt gcatgcaaga
      181 catataatgg ccaagcaacg aacataaatg agaattcaag tggttctgtt acaccagtaa
      241 agaatgaagc aaaagctgct gctaacatca aggaagctgt ttttgctttg ttttctggac
      301 gtgcatttct gtaaatagca tatgctcctg ctggaagccc gaacatcatg attgggaaaa
      361 atccagcttg atacattcct gttacgcctt ttgttccttc agaagccaaa aatttaccaa
      421 tatcattgat tcctgctacg tcaaaccaga acacggagtt cattgcttga tgcaatcctg
      481 tagggatcag taaacggttg aagaagccat aaagacctgc accaccccag cctaaactca
      541 aaatgaattt agcaaaagaa actaaagcag agtaaaccgg aggccatacg agcagtagca
      601 ctgcactgat cagcaacatg acaaaggcag ccaagattgg cactagacgc ttgccgctaa
      661 agaaagataa agccatcggt aattttgctt cgtggaaacg attataaaga gctgccgcaa
      721 tcaatccggc aataatacca ataaatacgt tactgatctg agcaaaagat ggatcaactg
      781 attctggttt gatattcaat aaagtagcta ctgaatcagt ttttaaaaca tttactggaa
      841 cttcaaatgc gaccaaacct gccaaggcag cagcacctga tttgtccact gacatcccta
      901 gtgctaaccc aacggcaaac agcaaaggta attgacctaa aactgcgtta ccgccagctt
      961 gtaaaaaatg agcgacaatg tcgttactat aagatgccca ccagtttccg atccctacta
     1021 gtaaagcagc agcaggtaag accgcaactg gaagttgaag ggaacgtccc attcgttgta
     1081 gatatgcttt caattttttt cctcctcatg attgttaaca tttgtttgta cgattgcgct
     1141 tcttaaacgc tcgatatgga tggccaagta acccagttct tctttcgtga tcgcgagctg
     1201 gtattctttg gtcagcaaca cacggatttt tttagaaatc ttatagcttt ctgggtattt
     1261 tcgtttgacc atttctacga tttccgtgtc taaaacagca tattgttgcg catgggttcg
     1321 ttgtagaagc agtcgtaaat gattcaccag tcttgaataa ttcagtgaaa atggttctga
     1381 atggatatct gtttccaatt ctgattccac taaatggatg atgtcagata caatcgtcac
     1441 ttcacggata ctgcgatggt tattgttccg accgcttcgt ccgctgtgaa tatggattgc
     1501 gatatatcct gcttcgtcat aagtatatgg aatcccaagc tcttttgtca gatactctac
     1561 tgcccattga gcaatactga attcatcact gtaaagcact tcgatttctc ttaaaagttt
     1621 gttgcggatg acgataccgt tattaatgtt ctccgcagaa aaagcaatgt ggtcggtcaa
     1681 agcgatcaat acatgttcat tcaacttttc catcaaaaca gtttccgcat gatcaatgat
     1741 cgtttctgct gcaaataaaa agcgttcatc aatctgatta agtaaatttt gcaacttgat
     1801 ttgaccttct ggttccatga caaatatccg ttcgacatca cgatcaaaaa tcaaatcatt
     1861 tctttttctg tcaaatccaa tcccttttcc aatagctact ttttcctgtc caccgtcatc
     1921 taccaatact gcattttgat taaagatctt tttgattttc atgcgcgcac cctttttgaa
     1981 gatttggttt tcacgtttgc tagaccagtt gattgcggcc aaacaaaaat agacacgaaa
     2041 aacctcctag ggaaaccatg ttcctcagag atttttcatg cctagtatga tctagtcaca
     2101 tgaaacgaat tattgttaac gtaatcaatt ttatatagac agttatgttt tgtcaattct
     2161 ttttttataa ttatagtaat ttactggaat tatcagaaat aaaaggaagg aacaaattaa
     2221 gaaacttatg ttatttaatc attggatctt ttgcttaact tattcgttgt taaaaagctg
     2281 aaagcgaagg aaaaaaactc tacttaactg tatttctccg ttaggacatc taaaaattta
     2341 cgagcagcag gagaaagagg ctggtctttt ttccaaatga gattcagtga agcttgtaat
     2401 ggcggatcga gtgggatgaa tactttttcc gtcccataag tattgatgat cccatctaga
     2461 gctaatgcac caaaagtccc gttatcgacc atcaaagaag cattgtacaa taagttatat
     2521 gttccgacga tcgataactg atctatgctg aaacctagcc attcagctag ttgactgtcc
     2581 actgacgttt gccgggaaat gatcaaagga acagttttca gttcctgcgg atggatgact
     2641 gtttgctttg ctaaaggatg attccgatgt atcaaaagtc cccaacgatc ttgggctggc
     2701 agtcgaaatg actggtattt ttgtttgttt gtcggttcga ttacgagtcc aaaatcaagt
     2761 aatccgctat ccagtttgtc tatgatatca tctgcattcc cgctatataa atgaaactgg
     2821 atatcaggat gcgccttgat caacgaatga aggctatcag aaatgaaaga gattgcttta
     2881 gtttcgcctc cgccaatgta gatttctcca gagatgattg cgtcatttgt taaattgttc
     2941 gccgtttttt ctaccaacga caagatttct tttccttttg aataaagata tcgaccagaa
     3001 tccgtcaact gtatctctcg ggagcctcga gtgaaaagtg tcacaccaag ttcttcttct
     3061 aaatctttca gttgttttga gagagtaggt tgcgtgatat gcagagattt tgctgctcgg
     3121 ctgatatttt tttctctagc tactgctaaa aaatacgtca gtatacgaat atccattttt
     3181 tccctcctgt ttttaaaaaa gcataccact aagtatagtt aaaaggaata gttaagtatg
     3241 ttttataagt attagacatc ataagccgtc cgttataaag taaggataaa aggagggagc
     3301 aaaatgacaa gcctcatcga aaaacaaatc gtcaataaag agattcgagt aacggatccg
     3361 ctctttgaag tgatccatca gatccaagcg gaaaacgaag agcctttagc agcgctgaat
     3421 actgggtacc atgaaccagc agatatccgc aaacggctag aaaatatcat tagtgacaaa
     3481 gtagatgata cggtgactgt tctgctgcct ttttatacag atttcggcaa acatattagt
     3541 atagggaaaa atgtctttat caatcgtcaa gtgatgttcg ttgacttagg tggcatctgt
     3601 ttagaagatt cagtattgat tggtcctcgt gtcaacctca tcacggtgaa ccatctaacc
     3661 gatccaaaag aacgaagagg cttgtcagta aagccaatcc acatcaagaa aaatgcttgg
     3721 atcggagcag gagcaactat tctgccaggc gtaacgattg gcgagaatgc cattgttgca
     3781 gcgaatgcaa ctgttacgaa agatgtccca gacaatacca tcgttgcagg aatccctgcc
     3841 aaaatcgtca aacctgtcga acggattatt tgaacagtaa gatgaaataa gagatagtga
     3901 gtataagcaa gagatttttc aaaacaggag gagaatagaa tgaatgattt atcagaaaaa
     3961 gtgattgtca tcatgggcgc ttcaagcgga atcggtgaag caacaacgaa gctattagca
     4021 gaaaaagggg ctaaactagt tatagctgca cgccgagaag atcgcctaaa agcaataaaa
     4081 gaatctttac cagaagctga gctatatatt caaacagcag atgtaagaga ttttgcacaa
     4141 gtccaagcag ttatcgattt agcgatggaa aagttcggtc gaatcgatgt attgtacaat
     4201 aatgcaggaa tcatgccaac cgctccatta gttgaaggtc accgtgatga atggcaaaat
     4261 atgcttgata tcaacatcat gggtgtgtta aatggaattt ccgccgtact tccaatcatg
     4321 gaaaaacaaa aatctgggca tatcatttca actgattctg tggcgggaca tgttgtttat
     4381 cctgattccg ccgtttattg cggaacaaaa tttgctgtcc gtgcaatcat ggaaggatta
     4441 cgtcaagagc aacggcaaaa taacatcaaa tctacgatca tctctccagg agctgttcaa
     4501 actgaattat atcaaaccat cagcgacaaa aaggctgctc ttgaacttca tgaagcgcaa
     4561 aaagaatggg gattaacatc tgaagatata gcttctgctg tagcatttgc gatagaaacg
     4621 ccagaccgga tgtctgtcag caacatgatt atccgtccga cgacacagga agtataatcc
     4681 ataacggata atatccagag agaattactt gttaaatagt tattcaaacg accatcaatt
     4741 taaggcgtga atggagaaga atattcttca tttacgtctt tttatgcaga aaaagcaaat
     4801 ttattaaaaa gaaacaaatg ttatttaaat atggacattg acttgctttt acttttttag
     4861 ttacactgtt atttaggaaa agtttcataa ttcaaaggag aaaaaagatg aaagcaaaga
     4921 atttattatt tagcgttatt cttttaggaa atctagcagt tgccaacaca acagtgctag
     4981 ctgaagaatc gacagaaact gtcgcaactc agacatcgag ttcttcagaa acaggaattg
     5041 aaaacgatac agaaagtact ttgcagtcag ctactgtctc agaaaataca atttcaaccg
     5101 aagaaccaac aactgaaaca acagaacaaa cacaaaccga agacagtaca tcagcagaag
     5161 ctcagaacaa tccagagaat gcagaagaaa tcacgaacca attgacagat gggcaaacat
     5221 tgacacatac ccatcaagca gatttgtctg aattcgaaaa ctatactggc tatgcttttt
     5281 atgctcgctc atctgctaat tctcaacaag cgttcattga tagtattgcc tcaactgcac
     5341 aaagtttggc aagtgccaat gacttatatg catcagtcat gattgctcaa gccatcgttg
     5401 aaagtggctg ggggaacagt actttggctt ctgcccctaa ttacaatcta tttgggatta
     5461 aaggaagcta taatggccaa tctgttacga tgcctacttc ggaatatgtg aatgggcaat
     5521 ggattacggt gaatgccgct tttagaaaat atccttcgta caaagaatca ttgcaagata
     5581 acgtaacggt attgaagacg acttcttttc agccaggcgt ttactactat tccggtgcat
     5641 ggaaaagcaa tacgaactct tacaaagatg ctacggcttg gctaacaggt cgttatgcca
     5701 ctgcgcctaa ttatggttct acattgaaca atgtaatcga aacctataat ctgacacagt
     5761 atgatacagc accaacttca acttctccga tgtatcgtct gtataaccgt catacaggag
     5821 aacatttata tacattgaat gccggagaaa aaaattatct gccaactgta ggctgggaat
     5881 atgaagggat tgcttggcaa gcaccaaaca gcggacaacc tgtctaccgt ttatacaatc
     5941 cttacagtgg agaccatcat tacacaatgg cacaatctga gatcaactct ttaacaaaaa
     6001 tcggttggcg ttatgaaggt ctaagttttt attctggtgg ttccaagcca atttatcgac
     6061 tatttaatcc aaatgaaaaa actggtaccc atcactatac cttgagtgct aaggaaagag
     6121 attggctaac accgatgggc tggagatatg aaggaatagg attttatggt tattaaaaag
     6181 aggtagtgaa aaagctgccc tgcatcaagt agtaagaaca gccaaacaaa aacagatttt
     6241 catgtttttt gtttggttga acttattacc gcagatgcaa gtttttgaac accgtttact
     6301 caaaaaaggt tgtgacaaca tcttgtcata acttttttat tactgtttaa gcatactgat
     6361 ccaatttcta atattcgacg ggattcttgc aaaaggcgat ttcggaaatt tttggcggtg
     6421 taaaattatc tactgcagaa gagctatctt ctatagtttg ataagaatca ataatcttgg
     6481 ttttagcgtt gtgctttctt tatcattttt tccgttcatt ctacaaaaaa tttcttttct
     6541 ctatactttt ctattgagag cgtttttttt attacacagc tgattctttg acttttttct
     6601 ttttttattc tattattgac aagtcaatcg ttgacgagtc aattattaga aaggaggaaa
     6661 ttatgtcttt agaagagatt agtcaccttt tatatcaaat caaattagcc gatcaaaaaa
     6721 tcgtccagat ctttgaagaa aatatcggta taagtcttac tagatacgag atcatgatga
     6781 gactgaaaga agaaaatcca ttgctacaaa gcagtttgca agaaaagttg cagattgatc
     6841 aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa tggctatgtt tcgcgtcaga
     6901 gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac agaaaaagcc cagaagaaaa
     6961 tcaagcattg tgaaatggac cataaagata caggttccgt gctatccccg gctttcagcg
     7021 atgtagaaat gaaacaattt ctaacacttt tagataaatt gaatacccaa ctttgcaata
     7081 taacaacaga aacgaaagag gttaataata atggaacaaa aaaatgattt ttcaacaatt
     7141 atgactggta gaagatccgt tcgcgtatat gatgaaacat acaaaatccc acacgaagaa
     7201 atgctggaaa tgatcaaaga agcgacgact gcaccttctt ctgtcaatat gaaaccatgg
     7261 cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct tgcgtccttt gatccgtttc
     7321 aatacgagac aaaatgatac ttcttctgca atggtcatga tttttggcga tatgcaatgt
     7381 tacgaatatg gcgaagaaat ctacaaccaa gcttacgaat cagggaagat gccaaaagaa
     7441 gtcagagatc aacaattagc tgcgatcatt ccttattaca agagtttttc aagagaagaa
     7501 atgaacgatg tagtcaaagt cgattctagc ttagcggcta tgcaattcat gttagttgcg
     7561 cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca gttggcagag
     7621 gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat aggaaaagcg
     7681 gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactat gttcgaataa
     7741 aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt ggtaacctaa
     7801 taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa cttttgaata
     7861 acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg agtatgtcca
     7921 ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat ttagtcatag
     7981 ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag atttttctgc
     8041 tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa
     8101 atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta
     8161 ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg
     8221 gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc
     8281 aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc
     8341 taaatttgga attagccagt catttccagt taagactgga agaaacttca gagcggaaat
     8401 ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag
     8461 ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg
     8521 aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag
     8581 atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga
     8641 attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag
     8701 agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag
     8761 cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ctggatcggt
     8821 tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat
     8881 tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc
     8941 gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg
     9001 tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag
     9061 gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata
     9121 tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc
     9181 aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag
     9241 agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt
     9301 tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc
     9361 tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg
     9421 caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt
     9481 ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga
     9541 aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat
     9601 ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg
     9661 atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt
     9721 catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt cattttggat
     9781 gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt aaatccagaa
     9841 agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat ggtgacatgt
     9901 atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt ctctgcttat
     9961 atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat catcgttggc
    10021 ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa cactgttatt
    10081 tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc tagaaaaata
    10141 gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt ccaactgtat
    10201 tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg ttctgtttat
    10261 cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt ggagttcctg
    10321 ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt gtttcttgtc
    10381 tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca gctgttgttc
    10441 tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag catagatttt
    10501 tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc attcctctac
    10561 aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct cgtctttgtc
    10621 caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga tctgactgtg
    10681 atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac gtggtttgga
    10741 taaaagcacg cataataata tataggctaa agtattcata aaaatcctac ctctcgtctt
    10801 attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat tgacatgtaa
    10861 aataaatcat gtatgatgct aatagagaga aagggatgga aatattggag aatcaaatag
    10921 atatacaaga acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag
    10981 ctacctgtat gatcgctttt atgggagtgg gtctagtaga cccaatctta aaaacgattg
    11041 ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt atttactagt tacatgttag
    11101 tcactggtgt ggtgatgcta tttacaggtt tcttatctag tcggatcggt ttgaaaaaaa
    11161 cattgatgac aggattgttt atcatcgttg tattcgctgg tcttggcgga ttatctggga
    11221 atatcggaat gctgattggg ttacgtgccg gctggggtgt cgggaatgct ttatttgtct
    11281 ctactgcttt agcaacgatc gtcagtattt tagttggtaa cacagaaaaa gcaatcatga
    11341 tgtatgaagc agcacttggc tgcggaatgg ctgttggccc tctagtcggt ggggtcttgg
    11401 gcagtggttc atggcgttat ccattttttg gggtagcatt tttgatgttt tgcggtttta
    11461 tagccctagc ggtactttta cctgaaactg agaaaccaaa gagaaaagta ggcctttggg
    11521 aaggtgtcaa agcgttaagc aatcataaac ttcgttcagt tggactaatc gcattgttat
    11581 ataatttcgg attctttact ttacttgcgt atgcgccatt tttattgatt ggctattctg
    11641 aattagaagt cggttttgta ttttttggct ggggtgtgct tctggctatt gcttcgattt
    11701 tcgttgcacc ggctcttgag cggaagttta cgacttatcg gacaatttta gcagcatttg
    11761 tcttattcat tatttgtttg ctgttattag gagctggaac ttctatgcca gtccttgtac
    11821 cgatcagcat tgtgttagca ggatttttcc aaggaattat caatacactg ttgacgacga
    11881 ttgctatgga aataccaggt ctgcaacgaa acgttgcttc gtccagctat agttttgtcc
    11941 gctttttcgg tggagcttta gcaccattta ttgcaggtgt gattggtgaa catattggtg
    12001 aaagctatgc gttttatttt gcaggtgcca ttgttcttat cagtgtatgt tgtatcattt
    12061 atcaccgtaa ttattttgct acaaatgaag aagaataaga aatcagaggt tgtgaaaaag
    12121 ctgtcctgca tcaagtcata agaa
//
LOCUS       DACCQP010000232         8624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.102, whole genome shotgun sequence.
ACCESSION   DACCQP010000232 DACCQP010000000
VERSION     DACCQP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.102"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..304
                     /locus_tag="IVO87_003047"
     CDS             <1..304
                     /locus_tag="IVO87_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7451148.1"
                     /translation="KKIEQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAIC
                     GSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            562..2064
                     /gene="lsa"
                     /locus_tag="IVO87_003048"
     CDS             562..2064
                     /gene="lsa"
                     /locus_tag="IVO87_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7451149.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2131..2658
                     /locus_tag="IVO87_003049"
     CDS             2131..2658
                     /locus_tag="IVO87_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7451150.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2869..3930
                     /locus_tag="IVO87_003050"
     CDS             2869..3930
                     /locus_tag="IVO87_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7451151.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3933..4829
                     /gene="murQ"
                     /locus_tag="IVO87_003051"
     CDS             3933..4829
                     /gene="murQ"
                     /locus_tag="IVO87_003051"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7451152.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4843..6825
                     /locus_tag="IVO87_003052"
     CDS             4843..6825
                     /locus_tag="IVO87_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7451153.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6890..7336)
                     /gene="nrdI"
                     /locus_tag="IVO87_003053"
     CDS             complement(6890..7336)
                     /gene="nrdI"
                     /locus_tag="IVO87_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7451154.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7547..>8624
                     /locus_tag="IVO87_003054"
     CDS             7547..>8624
                     /locus_tag="IVO87_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7451155.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDS"
BASE COUNT         2789 a         1503 c         1939 g         2393 t
ORIGIN      
        1 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
       61 gatccacgga aaagttccac acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
      121 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctctctgatt ttctcccaat
      181 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
      241 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
      301 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
      361 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
      421 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
      481 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
      541 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
      601 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
      661 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
      721 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
      781 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
      841 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
      901 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
      961 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     1021 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     1081 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     1141 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     1201 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     1261 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     1321 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     1381 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     1441 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     1501 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     1561 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     1621 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaatcccag
     1681 ccaaaacatc tgagcattag ttatgtacgc caaaattatg aagacaatcg aggaacgttg
     1741 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     1801 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     1861 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     1921 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     1981 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     2041 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     2101 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     2161 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     2221 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     2281 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     2341 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     2401 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     2461 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     2521 gaagacgaag aaaatgtcta tctgttagtt tgtcataatg ccttgataag agtgatcaat
     2581 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     2641 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     2701 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     2761 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
     2821 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
     2881 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
     2941 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
     3001 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
     3061 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
     3121 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
     3181 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
     3241 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
     3301 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
     3361 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
     3421 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
     3481 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
     3541 aatgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
     3601 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
     3661 gcaagagatg tcatacgaag cgcagatgca cgttttcgag agattccgcc tatcccagca
     3721 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
     3781 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
     3841 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
     3901 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
     3961 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
     4021 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
     4081 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
     4141 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
     4201 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
     4261 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
     4321 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
     4381 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
     4441 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
     4501 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
     4561 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
     4621 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
     4681 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
     4741 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
     4801 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
     4861 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
     4921 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
     4981 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
     5041 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
     5101 gtgaaattag gagaaaaact ttcttctgta agtgcagcta cagcaaattc atcagcatct
     5161 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
     5221 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
     5281 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
     5341 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
     5401 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
     5461 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
     5521 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
     5581 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
     5641 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt
     5701 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
     5761 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
     5821 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
     5881 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
     5941 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
     6001 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
     6061 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
     6121 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
     6181 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
     6241 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
     6301 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
     6361 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
     6421 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
     6481 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
     6541 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
     6601 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
     6661 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
     6721 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
     6781 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
     6841 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
     6901 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
     6961 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
     7021 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
     7081 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
     7141 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
     7201 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
     7261 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
     7321 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
     7381 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
     7441 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
     7501 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
     7561 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
     7621 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
     7681 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
     7741 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
     7801 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
     7861 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
     7921 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
     7981 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
     8041 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
     8101 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
     8161 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
     8221 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
     8281 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
     8341 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
     8401 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
     8461 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
     8521 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
     8581 atgtacgaat gtctttttac tttcattatt attcactgat agtg
//
LOCUS       DACCQP010000233         7272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.111, whole genome shotgun sequence.
ACCESSION   DACCQP010000233 DACCQP010000000
VERSION     DACCQP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.111"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1020
                     /locus_tag="IVO87_003055"
     CDS             <1..1020
                     /locus_tag="IVO87_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7451156.1"
                     /translation="TLIITTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDL
                     MSMFSLQSNDSQSVGSRMMRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKG
                     QSGSGSSASTQADHSRPNGQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLP
                     TNARYAVYQGKSKVTDNVHDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVK
                     QKKQPASSVHERPATKQEQSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTE
                     RQSNTVQERTVQKPVTSTAPTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKK
                     GSKKP"
     gene            1017..2018
                     /locus_tag="IVO87_003056"
     CDS             1017..2018
                     /locus_tag="IVO87_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7451157.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            2033..2947
                     /locus_tag="IVO87_003057"
     CDS             2033..2947
                     /locus_tag="IVO87_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7451158.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            3192..3308
                     /locus_tag="IVO87_003058"
     CDS             3192..3308
                     /locus_tag="IVO87_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7451159.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3324..5243
                     /gene="tet(M)"
                     /locus_tag="IVO87_003059"
     CDS             3324..5243
                     /gene="tet(M)"
                     /locus_tag="IVO87_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7451160.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            5341..6714
                     /gene="tet(L)"
                     /locus_tag="IVO87_003060"
     CDS             5341..6714
                     /gene="tet(L)"
                     /locus_tag="IVO87_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7451161.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2337 a         1243 c         1515 g         2177 t
ORIGIN      
        1 acattgatta ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt
       61 tatccgttct ttttgattgc ttttctacag attgtgacct ttgcaggaat ctactttaag
      121 ctgggcgatt taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt
      181 cgtatgatga gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa
      241 cttggacgct ccgtgactgc tttaggggct gggtctgcca ttgccagtgc tacgggtaaa
      301 aaaggacagt cgggttcggg aagttctgcg agtacacaag cagatcactc tcgaccaaac
      361 gggcaggaaa aatcaacact aggaaagcgt atcggtcaaa ccatcggtac agtagctgat
      421 accaaagaca gaatggtaga tactgctggt aatttgaaag aacaggttaa ggatttgccg
      481 accaatgcaa gatatgcagt atatcaagga aaatctaaag tgacggataa tgtccatgat
      541 ttaaccagaa gtatttctca aaccaaagcg gataaagcca gtggacgcaa gaaacagcag
      601 gaacaaagac ggaaaaccat tgcggagcgt cgctctgaaa tggaacaggt caaacagaaa
      661 aaacagccag cttcttccgt tcatgaaaga ccagccacta agcaagaaca atctcatgat
      721 ggacagactt caaggcaaac aactgtacag gcttcctata gggaatctca acaagccaaa
      781 caagagcgtc caacagttaa gtccgattct tcaagtttaa aaacggaacg tcaaagtaat
      841 acagttcaag aaagaacggt tcaaaagcca gtaacttcaa ctgcaccaac agatagagct
      901 tcacaacgtt caatcacaaa agaacgtcag tctactgttc aaagagtacc actacaaaat
      961 acaaaaagta gaccaccaat caaaaccgcc accattaaga aaggcagtaa gaaaccatga
     1021 agctgaaaac tttagtgatt ggtggttcgg gattattctt gatggtcttc tcactgcttc
     1081 tgtttgtcgc cattttattt tcagatgaac aagatggcgg tttttccaac attcactatg
     1141 gcggtgtgga tgtttccgca gaagtgctgg ctcataagcc tatggtagaa aaagttgcca
     1201 aagaatatgg aattgaagaa tacgtcaata tacttcttgc gattatacag gtggaatcgg
     1261 gtggtacagc agaagatgtt atgcagtcct cggaatccct cggtcttcca cctaattcat
     1321 tgagtacaga agaatccatt aagcaaggtg tgaagtattt cagtgagtta ttagccagta
     1381 gcgaaaggct tggtgtagat ttagaatcgg ttatccagtc ctacaattat ggtggtggtt
     1441 tcttaggata tgtggctaat cgtgggaata aatatacctt tgaactggct caaagtttct
     1501 caaaagagta ttcgggtgac gaaaaagtgt cttaccccaa tcccatagcc atacctatca
     1561 atgggggctg gcgatacaat tacgggaata tgttttatgt gcaactggta acgcagtatc
     1621 ttgtcacaac agagtttgat gatgatacag tacaagccat catggacgaa gctctgaaat
     1681 ttgaaggctg gaagtatgtc tatggcggag cttccccgac tacctctttt gattgtagcg
     1741 gactgacaca atggacgtat ggaaaagctg gaatcaactt accacgaaca gcacaacagc
     1801 aatatgatgt aacccggcat atcccattat tggaagcaaa agccggcgat ttggttttct
     1861 ttcattctac ctataacgct ggctcttata ttactcatat cgggatttat ttaggcgata
     1921 accgtatgtt tcatgcaggc gaccctatcg gttatgccga cttaacaagc tcttactggc
     1981 aacagcattt agtgggagca ggacgaatca aacaataaga aaggacgatt caatgatgaa
     2041 attcggaaaa aatcagaata cagaaaaaca gacaccaaag gaaaagaaac ctcgtgtcta
     2101 taaggtcaat cctcgtaaaa aggttgtgat tgtcctatgg gtacttttag gacttagttt
     2161 cagctttgcg atattcaagc actttacagc cattgatacc cataccattc atgaaaccac
     2221 catcatagaa aaggaatatg tcgatactca tcatgtggaa aattttgtag agaactttgc
     2281 gaaagtctac tattcatggg aactaaacga caagtccatt gataatcgca tagaaaacct
     2341 gaaagcatat ctgacagagg aacttcaagc tctgaatgtc gatacagttc gtaaagatat
     2401 tcctgtatcg tcttctgtaa aaggatttca gatatggacg gtagaagcag atggcgacaa
     2461 tcaatttgat gtaacctaca gtgtagacca actcattaca gagggggaaa atacaaagac
     2521 cgtccactct gcttatatag tgagtgtcta tgtagacagt actggaaata tggtcctcat
     2581 taagaatccg accattacta gcataccaaa gaaatcagac tataaaccaa aagctattga
     2641 aagtgagggt acggttgatt ccattacaac caatgaaatc aatgagtttt taacgacgtt
     2701 cttcaagctc tatcctacag cgacagccag tgaactttcc tactatgtga atgacgggat
     2761 attaaaacca atcggaaaag agtacatctt tcaagaactg gtaaatccta tttacaatcg
     2821 taaggataat caagtcacgg tatcgctgac agtggagtat atcgaccagc agaccaaagc
     2881 aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat
     2941 agaataacaa atattggtac attattacag ctattttgta atcacgtact ctctttgata
     3001 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tatctattta
     3061 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg
     3121 aacaaataat tggatgtcct tttttaggag ggcttagttt tttgcaccca gtttaagaat
     3181 acctttatca tgtgattcta aagtatccgg agaatatctg tatgctttgt atgcctatgg
     3241 ttatgcataa aaatcccagt gataagagta tttatcactg ggatttttat gcccttttgg
     3301 gcttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg
     3361 atgcgggaaa aactacctta acagaaagct tattatataa cagtggagcg attacagaat
     3421 taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag
     3481 gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca
     3541 tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag
     3601 atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat
     3661 ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa
     3721 atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg
     3781 taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg
     3841 aacaatggga tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta
     3901 aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgctcct
     3961 tgtaccctgt ttatcatgga agcgcaaaaa gcaacatagg gattgagcag cttatagaag
     4021 tgataacgaa taaattttat tcatcaacat acagaaagaa gtctgaactt tgcggaaatg
     4081 tcttcaaaat tgaatattcg gaagaaagac aacgtcttgc atatgtacgc ctttatggcg
     4141 gaatcctgca tttgcgggat tcggttagaa tatcggaaaa ggaaaaaata aaaattacag
     4201 aaatgtatac ttcaataaat ggtgaattat gtaaaattga taaggcttat tccggggaaa
     4261 ttgttatttt gcaaaatgag tttttgaagc taaatagtgt tcttggagat acaaagctat
     4321 tgccacagag agagagaatt gaaaatccgc tccctctgct gcaaacaact gttgaaccga
     4381 gcaaacctca acaaagggaa atgttacttg atgcactttt agaaatctcc gacagtgacc
     4441 cgcttctaca atattatgtg gattctacga cacatgaaat tatactttct ttcttaggga
     4501 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     4561 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     4621 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     4681 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat
     4741 ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga
     4801 atgtgacgga ttgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
     4861 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     4921 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     4981 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     5041 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     5101 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     5161 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     5221 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     5281 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggaattaatt
     5341 atgaagtgta atgaatgtaa cagggttaaa tacaaccaaa ttttaatttg gctttgcgtt
     5401 ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca
     5461 aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt tatgttaacc
     5521 ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta
     5581 ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt tggccattct
     5641 ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca
     5701 gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg taaagcattt
     5761 ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg
     5821 atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac aattatcact
     5881 gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc
     5941 aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat
     6001 agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg
     6061 aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga
     6121 gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat
     6181 atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat tattttccct
     6241 ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt
     6301 cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc
     6361 tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg
     6421 ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa
     6481 gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt
     6541 gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa
     6601 gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc
     6661 attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt
     6721 aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa
     6781 tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt
     6841 atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat
     6901 tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg
     6961 caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt
     7021 gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg
     7081 tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg
     7141 tcggcactgc cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta
     7201 tactttactt ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa
     7261 aaaggagcga tt
//
LOCUS       DACCQP010000234         6550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.115, whole genome shotgun sequence.
ACCESSION   DACCQP010000234 DACCQP010000000
VERSION     DACCQP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.115"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            241..2688
                     /locus_tag="IVO87_003061"
     CDS             241..2688
                     /locus_tag="IVO87_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7451162.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGESNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2918..3985
                     /gene="rlmN"
                     /locus_tag="IVO87_003062"
     CDS             2918..3985
                     /gene="rlmN"
                     /locus_tag="IVO87_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7451163.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4178..5281)
                     /locus_tag="IVO87_003063"
     CDS             complement(4178..5281)
                     /locus_tag="IVO87_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7451164.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5834..6382
                     /gene="aac(6')"
                     /locus_tag="IVO87_003064"
     CDS             5834..6382
                     /gene="aac(6')"
                     /locus_tag="IVO87_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7451165.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2193 a         1119 c         1403 g         1835 t
ORIGIN      
        1 cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata
       61 cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga
      121 aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca
      181 aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa
      241 atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa
      301 aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct
      361 gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc
      421 attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg attttggatt
      481 gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc
      541 ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg
      601 gaatcaaata tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa
      661 gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc
      721 ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc
      781 ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga
      841 gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa
      901 gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct
      961 gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat
     1021 actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca
     1081 atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca
     1141 ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc
     1201 tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt
     1261 gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa
     1321 aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa
     1381 gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta
     1441 agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg
     1501 atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc
     1561 agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta
     1621 gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg
     1681 cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt
     1741 atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg
     1801 aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg
     1861 aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat
     1921 gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag
     1981 aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat
     2041 gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta
     2101 gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg
     2161 gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca
     2221 agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga
     2281 ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc
     2341 atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat
     2401 tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca
     2461 caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt
     2521 tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa
     2581 ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt
     2641 gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat
     2701 gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa
     2761 atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag
     2821 attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag
     2881 tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg
     2941 cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc
     3001 tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa
     3061 tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca
     3121 gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa
     3181 aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac
     3241 acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg
     3301 tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga
     3361 gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga
     3421 taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat
     3481 tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca aggaattcgc
     3541 taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg
     3601 aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga
     3661 tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt
     3721 aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt
     3781 agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag
     3841 tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt
     3901 gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca
     3961 aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag
     4021 gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga
     4081 aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt
     4141 tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt
     4201 gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt
     4261 ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga
     4321 ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt
     4381 tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata
     4441 aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa
     4501 cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa
     4561 cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg
     4621 gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc
     4681 acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag
     4741 aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat
     4801 caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa
     4861 agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg
     4921 aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga
     4981 cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga
     5041 cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga
     5101 gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg
     5161 aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg acgattccat
     5221 ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca
     5281 tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga
     5341 ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc
     5401 atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa
     5461 aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg
     5521 taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa
     5581 ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt
     5641 ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata
     5701 atttttttat tttataagta agataaataa tgaaagaata gagatactga ttggtagcta
     5761 aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca
     5821 agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca
     5881 gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga
     5941 agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt
     6001 agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt
     6061 agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca attacttaga
     6121 aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca
     6181 tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat
     6241 ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg
     6301 tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat
     6361 tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa
     6421 ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg
     6481 aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag
     6541 tagtaagaaa
//
LOCUS       DACCQP010000235         4005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.145, whole genome shotgun sequence.
ACCESSION   DACCQP010000235 DACCQP010000000
VERSION     DACCQP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.145"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IVO87_003065"
     CDS             <1..177
                     /locus_tag="IVO87_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7451166.1"
                     /translation="SSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQ
                     EEGLDFFFFGNKLN"
     gene            208..450
                     /locus_tag="IVO87_003066"
     CDS             208..450
                     /locus_tag="IVO87_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451167.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGVSQTKRY"
     gene            845..1318
                     /gene="dfrF"
                     /locus_tag="IVO87_003067"
     CDS             845..1318
                     /gene="dfrF"
                     /locus_tag="IVO87_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP7451168.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1445..2101
                     /locus_tag="IVO87_003068"
     CDS             1445..2101
                     /locus_tag="IVO87_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP7451169.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            2283..2813
                     /locus_tag="IVO87_003069"
     CDS             2283..2813
                     /locus_tag="IVO87_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7451170.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            3359..3601
                     /locus_tag="IVO87_003070"
     CDS             3359..3601
                     /locus_tag="IVO87_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP7451171.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            3598..3957
                     /locus_tag="IVO87_003071"
                     /pseudo
     CDS             3598..3957
                     /locus_tag="IVO87_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT         1439 a          527 c          822 g         1217 t
ORIGIN      
        1 agcagctggt acttcttagg gattcaagtg attggtgtca tcattactgc cgtgtatgcc
       61 tatgtgctga caacagtgat tttaaaatcg gcgatgcact ttacaacgat cactactaca
      121 aaagaagaac aagaagaagg attggacttt ttcttctttg gcaacaaact caattgaaac
      181 aaattcaaat agaaaagagg aagattcatg caacaaagca aaacgaaaat tcctgcaaaa
      241 aaattaacgt tctttggctt tttggctatg accatctcaa tggtcgtttc attatatgaa
      301 tatcccacat tcgcgacctc aggcttcagc ttagtatttt tcttgttatt aggcggtttg
      361 ctatggttta tccctgtggc attgtgcgca gcagagatgg caacggtgaa aggatgggaa
      421 aaaggtgttt cccaaacaaa aagatattaa gaaacagccc atgtttacct gcttgcaaac
      481 aaaagaatat tgctactaga ctaagagata ctacataagt gtgttggtat ctcttttttg
      541 ttgccgtgaa acagcagact atttcatttc tgcttgatta ccttaaaact tattagtcta
      601 tggtttgtca agggcttgtt gcgtgagaaa tgctgaatat caccctttac aaaacatagg
      661 ctgataagta acttttcaaa agcagaaaag aaatggcaat gcttataaca atatgatata
      721 atgcagatga agacaaaaaa ggaatttgtc gagctggtta gttcgagata attttgagtt
      781 tgttaagatg tggtgcactt gatggaatca caggtacgac atcatattat ggaggatgaa
      841 atagatgata ggtttgattg ttgcgaggtc aaagaataat gttataggca agaatggtaa
      901 tataccatgg aaaataaagg gagaacaaaa gcaatttaga gagttaacaa cgggtaatgt
      961 ggttattatg gggcgaaagt cttatgaaga aatcggtcat ccgttgccta atagaatgaa
     1021 tattgttgtt tccaccacaa cagagtatca aggagataat ttagtttcag ttaaatcatt
     1081 agaagatgca ttattattgg ctaaaggacg agatgtatac atatctggtg gatatggact
     1141 atttaaggaa gctttgcaaa tagtagataa aatgtatatc acagaagtag atttaaatat
     1201 tgaagatgga gatacattct ttccagaatt tgatatcaat gattttgaag ttttgatagg
     1261 ggaaacactt ggtgaggaag tgaaatatac gagaacattt tatgtaagga aaaaatgaat
     1321 tgagtaaatt ttggatttag ggtgttttca taaatatatt gctttttatg catatataaa
     1381 ttgttgtaag acaaattcca gtttgtatgg ggcgataaaa tcagaatttg agagaggatt
     1441 gaatatgaat aaggaatggt ctgaacttaa taaaacaatg caggcacaaa taaaaaagaa
     1501 agatacttat aagaggggta ttgatacttt acttacttta cgaagtcagt taatacaaac
     1561 cttagtatct ttcaaagaag aattatgcag agaagatttt aactcaatcc ccttcataaa
     1621 cgctgatggt tatcatagta agacgattgc ttattctatt tggcatattt tccgcattga
     1681 agatatagtt gtgcatacag tgataaatga agatgaacaa gtattttttg caggcaatta
     1741 tcaagagcgt atcaattcac ctatcattac aacaggaaat gaactcatga aacagcagat
     1801 tgcagacttt tcaaaacagc ttaatctgga agaattatat ttatatattt tcgaagtatg
     1861 ggaaagcact gagaaaatgc ttgaacggtt atcttatgat gaattgaaaa gaaaaatacc
     1921 gaaagaaaga aaaggatact tagaatcgtt gaatgtagta aacgacaatg aaaaagcaat
     1981 ttggctgatt gattattggt gtaataaaga tatttgtgga ctaattcaaa tgccgttttc
     2041 aagacattgg attatgcaca cagaagcctg tttgcgtata aagaacaaga tacattcata
     2101 ggcaaaaatt tcagtttgta gaactaagga aattggaatt taaggaggaa taaagtatgg
     2161 ttagtgattt tataagagca gcatttccat ggattttaat gggtttattt gtagcaatca
     2221 gttgttccct tatgagtaaa aaagagaaat aaattcctgt ttgtatttat aaaagagaaa
     2281 aaatgagaaa agtagtttta tttattgcca tgagccttga cggatatatt gcggacggta
     2341 atggtggagt agcttggcta aatggtcatg gaaatgacaa tgaaaacatc gacacttata
     2401 cagaatttac caaagatata gatactgtct taatggggtg gaatacttct catcaggttg
     2461 ttactgaact ttctccgcaa gaatgggtat ataataaatt tacaacttat gtattaacac
     2521 ataaagagtg caattcttcc caacaaattc attttacaag tgaaaatcct gttttattat
     2581 tggaacggct aaaacaagaa gcaggaaagg atatttggat ttgtggcgga gcaaatcttg
     2641 ttcagcagtt ggtaagcaaa aatatgattg ataaatatta catttctgtt attcctactc
     2701 tgttaggaaa tggggttcgt cttttgggca acatagacag ggaaataaag ttaaggcttt
     2761 gtaaaacaca gacttataac ggaataaccg atttgattta tatgcgtaga taactagaga
     2821 gaaacagaaa agctgttaaa ccgacggagc gatccgacca cgttggcggg aaaatcctta
     2881 agatttttcc gccatgcttg caagggggcg gttcgcttcg gcggttcggc gagcttgcga
     2941 gccgttcaaa gcccccgtta tctaacaagg ggggcacaaa aaacaagaaa cactatacac
     3001 taacaagcgg aaaacgctgt atttataagg caaaacccca tttttacagt agtgatataa
     3061 attgtaccta tatcactatg aaaattgaat agtaaatatg agaaagacag ttgatttcaa
     3121 aaaataagaa attgattgtc gttttctttt gcagaaattt aagtgcaggc aattatggta
     3181 gttgtcgtaa ttgctgtcag ctcagtgttt atcactgatg gtgtgaaaga tttacggttg
     3241 gagatgttca ataagattat tggtgcttaa ttatttttat tgtagtgctg atatgagaat
     3301 gttataattg gggtataaaa catacaaata aatttgctga aaaatgaggt atcaaaatat
     3361 ggaaaatgaa ttactggaaa acttaggaag acttcacaca actgaacttg gcgttattag
     3421 aattaaaaag aacttatctt tgaatgtaga aaatgtaatt gaatggtgca aagaaaaaat
     3481 tagcctttct aatgcggaaa tcacaagaaa aggaaaaaac tggtatataa cgattgataa
     3541 ttgtattata acggtaaatg cgtacagtta taccgttatc actgctcaca aggtaaaatg
     3601 ataaattttt ctataaagga attaaacgca tttgcagtta ttgggcagga agtagaactg
     3661 actaattatc aaaaaaagaa tattcagatt agtacacagt tttggagaaa gtttaacagt
     3721 agtttgaaaa aagcgtatct ttcacaatca ggaaattggg taaaatatgc ttttatggaa
     3781 agaagaaatg gaaaacttta ttatttctgt tctattcccc aaagaaccat taccccagag
     3841 ggctttctgt ataaagaaat accgtcttat aaatatttgg ttgtggagca tattggtgct
     3901 atggataaaa tatatgaaac ttatggcaag atttatgaag aaattattcc tagtacatcg
     3961 tatattggct ctttgtcaaa tgatgttggt aaagagaaat cttag
//
LOCUS       DACCQP010000236         3868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.148, whole genome shotgun sequence.
ACCESSION   DACCQP010000236 DACCQP010000000
VERSION     DACCQP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.148"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..128
                     /locus_tag="IVO87_003072"
     CDS             <1..128
                     /locus_tag="IVO87_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7451172.1"
                     /translation="MNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            140..1087
                     /locus_tag="IVO87_003073"
     CDS             140..1087
                     /locus_tag="IVO87_003073"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7451173.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1107..1634
                     /locus_tag="IVO87_003074"
     CDS             1107..1634
                     /locus_tag="IVO87_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7451174.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1697..2350)
                     /locus_tag="IVO87_003075"
     CDS             complement(1697..2350)
                     /locus_tag="IVO87_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7451175.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2482..3324
                     /locus_tag="IVO87_003076"
     CDS             2482..3324
                     /locus_tag="IVO87_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7451176.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3482..>3868
                     /locus_tag="IVO87_003077"
     CDS             3482..>3868
                     /locus_tag="IVO87_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7451177.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLT"
BASE COUNT         1383 a          689 c          793 g         1003 t
ORIGIN      
        1 agatgaatca tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg
       61 aaacagaagc acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag
      121 aagattagga ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga
      181 agaagggcat cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca
      241 aatgcggttt gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg
      301 attgatcaaa agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt
      361 acaaaataaa aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga
      421 ctataatgga ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa
      481 tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt
      541 tgctgaaaca tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac
      601 aaaagacggt ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc
      661 tgattctaga agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt
      721 gcctccttgt catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt
      781 gtatcaacgc agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt
      841 gctgactcac ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt
      901 aggagatgct catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga
      961 aatacgcgca tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga
     1021 gatggaagat atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc
     1081 tgtttagaat cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg
     1141 gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca
     1201 aacaaatgac agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta
     1261 gccgtccgtt gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag
     1321 aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg
     1381 gtatcttttt catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga
     1441 tattataccg tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg
     1501 actggtctga ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt
     1561 atccgcatca atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg
     1621 aattgacgat ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat
     1681 agctttattt tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta
     1741 acataacaaa caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga
     1801 atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa
     1861 gagagtgata aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga
     1921 tcgtagatac ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc
     1981 caagaatcgc aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac
     2041 tcagcaaaca aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga
     2101 atacttggaa cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta
     2161 aaaataaaag aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat
     2221 gcaagtgggc accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa
     2281 ttccatgggt gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg
     2341 ttttttccat gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct
     2401 gttatactaa aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta
     2461 aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg
     2521 atggaaaaaa atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc
     2581 gacggaacag tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg
     2641 gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa ttggagaatt catctcgatg
     2701 tatgatgagc taggagaaga cggcagtgaa gtgatttcga tccatatgac aaaaggcttg
     2761 agtggaacag tagaagcagc aagacaagcc agtaatctaa caaaaacgaa agtaacggtt
     2821 atcgacagtg ataccactga ccaaggatta tcttttcagg taattcgagc agctgaatta
     2881 gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag tcaaaaaagt acaagacaat
     2941 acaaaattat acatcggaat atccacacta gataacttgg taaaaggtgg aagaatcagc
     3001 cgaacaactg gtttgctgtc tagtgttttg aacatacgcg tggtgatgaa tttcgcccat
     3061 tctgaattaa tccctgtaac taaggggcga ggaaagaaaa cgttcgataa atggtttaat
     3121 aatttgaaaa atgaactgaa agaattgcca aatgtgcgac aaatcggtat ttcacatgcg
     3181 gatgcaaaag aattggcgca agaattcaaa gagggtttgc aagaattatt cccggacatg
     3241 catatccctg tactgcatac gaatccggtt atcgctacgc atactggtaa agacgcgttt
     3301 gcaatcatgt attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg
     3361 cagcttattc tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag
     3421 actacctttt tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa
     3481 aatgaaatta acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat
     3541 catctatgca ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac
     3601 tactgtgaga gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc
     3661 gctgactgaa ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa
     3721 tgatctaaaa gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg
     3781 tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat
     3841 caaaacagct gattttatca cactgact
//
LOCUS       DACCQP010000237         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_089
            SAMN12916546-rid10366793.guided.201, whole genome shotgun sequence.
ACCESSION   DACCQP010000237 DACCQP010000000
VERSION     DACCQP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916546
            Sequence Read Archive: SRR10233592
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820147.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,946
            CDSs (with protein)               :: 2,946
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 29 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 7 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916546-rid10366793.guided.201"
                     /isolate="RPH_EFM_089"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="IVO87_003078"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="IVO87_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP7451178.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            187..921
                     /locus_tag="IVO87_003079"
     CDS             187..921
                     /locus_tag="IVO87_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP7451179.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          413 a          133 c          140 g          339 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaaggagtgg
      181 gttataatga acaaaaaaaa tataaaagat agtcaaaact ttattacttc aaagcatcat
      241 ataaatgaaa ttttgagaaa tgtacattta aatacaaatg ataatattat tgagattggt
      301 tcagggaaag gtcatttcac gtttgaatta gctaaaaggt gtaattatgt aaccgccatt
      361 gaaatagatc ctaaattatg taggataact aaaaacaaac ttattgaata tgagaacttc
      421 caggttatca ataaagatat tttacaattt aagtttccta aaaataagtc atataagata
      481 tatggaaata taccctacaa tataagtaca gatataattc gaaaaattgt ttttgaaagc
      541 acagctacag aaagttattt aatagtggaa tatggatttg ctaaaaggtt gctaaataca
      601 aatcgttcac tagcactatt tttaatgaca gaagttgata tatccatatt aagtaaaatc
      661 cctagagaat actttcatcc aaaacctaga gttaatagct cgttaattgt attaaaaaga
      721 cacccttcaa aaatatcact caaagataga aaacaatatg aaaattttgt tatgaaatgg
      781 gttaacaaag aatacataaa actattttcc aaaaaccaat tttatcaagc cttaaaatat
      841 gcaagaattg acgatttaaa caatattagc tttgaacaat tcttgtctct tttcaatagc
      901 tataaattat ttaatagata agttaagggg tgcataaact gcatccctta acttatctta
      961 ttttgcttaa acaatcttgg cccgtaaact gcataactca tatgtaaatc gctccttttt
     1021 aggtg
//
